摘要
目的:探讨长链非编码核糖核酸生长组织特异性转录体5(LncRNA-GAS5)在食管鳞癌组织中的表达及其对食管鳞癌细胞株EC9706细胞增殖、凋亡的影响。方法:选取2016年2月至2019年1月于中国人民解放军陆军第958医院诊治的食管鳞癌患者78例,比较LncRNA-GAS5在食管鳞癌组织及癌旁组织中的表达差异;过表达质粒(pEGFP-C1-GAS5)转染EC9706后通过CCK-8试剂盒以及平板克隆试验检测LncRNAGAS5过表达对细胞增殖能力的影响,采用Annexin V/PI双染流式细胞术检测LncRNA-GAS5过表达对细胞凋亡的影响,荧光实时定量聚合酶链反应(qRT-PCR)及蛋白质免疫印迹法检测抑癌基因PTEN表达变化。结果:与癌旁组相比,食管鳞癌组LncRNA-GAS5相对表达量降低(P<0.05);与空白组、阴性对照组相比,pEGFP-C1-GAS5组LncRNA-GAS5相对表达量升高(P<0.05);与空白组、阴性对照组相比,pEGFP-C1-GAS5组转染后48、72、96 h OD值、细胞克隆数降低(P<0.05),p EGFP-C1-GAS5组细胞凋亡率升高(P<0.05),PTEN mRNA、PTEN蛋白表达升高(P<0.05)。结论:食管鳞癌患者癌组织内LncRNA-GAS5低表达,LncRNA-GAS5过表达抑制癌细胞增殖,促进癌细胞凋亡,可能是通过促进抑癌基因PTEN表达实现的。
Objective:To investigate the expression of long non-coding ribonucleic acid growth tissue-specific transcript 5(LncRNA-GAS5)in esophageal squamous cell carcinoma and its effects on the proliferation and apoptosis of EC9706 cells.Methods:78 patients with esophageal squamous cell carcinoma were selected from February2016 to January 2019 in 958 Hospital of the Chinese People’s Liberation Army,the expression of LncRNA-GAS5 in esophageal squamous cell carcinoma and adjacent tissues was compared;after transfection of EC9706 with p EGFP-C1-GAS5,the effect of over expression of LncRNA-GAS5 on cell proliferation was detected by CCK-8 kit and plate cloning assay,Annexin V/PI double staining flow cytometry was used to detect the effect of overexpression of LncRNA-GAS5 on cell apoptosis,the expression of PTEN was detected by quantitative real time polymerase chain reaction(qRT-PCR)and western blotting.Results:Compared with the adjacent group,the relative expression of LncRNA-GAS5 in esophageal squamous cell carcinoma group was lower(P<0.05);compared with blank group and negative control group,the relative expression of LncRNA-GAS5 in p EGFP-C1-GAS5 group increased(P<0.05);compared with blank group and negative control group,OD value and cell clone number in pEGFP-C1-GAS5 group decreased at 48,72,96 hours after transfection(P<0.05),the apoptotic rate of pEGFP-C1-GAS5 group was increased(P<0.05),the expressions of PTEN mRNA and PTEN protein increased(P<0.05).Conclusion:The expression of LncRNA-GAS5 in esophageal squamous cell carcinoma is low,LncRNA-GAS5 overexpression inhibits the proliferation of cancer cells and promotes the apoptosis of cancer cells,probably by promoting the expression of tumor suppressor gene PTEN.
作者
裴家强
游波
游凯
吴蔚
PEI Jiaqiang;YOU Bo;YOU Kai;WU Wei(Department of Cardiothoracic Surgery,958 Hospital of the Chinese People’s Liberation Army,Chongqing 400020,China;Department of Thoracic Surgery,Southwest Hospital Affiliated to Land Force Military Medical University,Chongqing 400038,China)
出处
《现代医学》
2020年第7期850-855,共6页
Modern Medical Journal
关键词
长链非编码核糖核酸生长组织特异性转录体5
PTEN基因
食管鳞癌
增殖
凋亡
long non-coding ribonucleic acid tissue-specific transcript 5
PTEN gene
esophageal squamous cell carcinoma
proliferation
apoptosis
