摘要
目的:了解在种植窗期中的子宫内膜中的雌激素受体α(ER-α)、孕激素受体(PR)及端粒酶催化亚基(TERT)的表达情况,探讨发生反复种植失败(RIF)的可能原因。方法:选择2014年11月至2019年5月在暨南大学附属深圳市宝安区妇幼保健院生殖健康科及妇科因试管婴儿RIF者45例(RIF组)及正常妊娠女性30例(正常妊娠组)作为研究对象,检测两组患者在种植窗期子宫内膜的厚度、血清雌二醇(E_(2))、孕酮(P)的水平以及子宫内膜中TERT、ER-α、PR表达情况。结果:RIF组与正常妊娠组在种植窗期的血清E_(2)、P水平比较,差异无统计学意义(P>0.05),但RIF患者子宫内膜厚度偏薄,差异有统计学意义(P<0.05);RIF组种植窗期子宫内膜中的TERT表达较正常妊娠组明显升高,ER-α表达低于正常妊娠组,PR表达高于正常妊娠组,差异均有统计学意义(P<0.05);TERT表达与ER-α(r=-0.6964,P<0.001)、PR(r=0.3471,P=0.0195)的表达均有显著相关性;在种植窗期血清中E_(2)/P值在两组比较中差异无统计学意义,但RIF组中子宫内膜中的ER-α/PR值在种植窗期表达下降,差异有统计学意义(P<0.001)。结论:子宫内膜偏薄,子宫内膜中ER-α、PR的表达失调以及TERT表达增强是引起种植失败的可能原因。
Objective:To investigate the expression of estrogen receptorα(ER-α),progesterone receptor(PR)and telomerase catalytic subunit(TERT)in endometrium during implantation window,and to explore the possible causes of repeated implantation failure(RIF).Methods:From November 2014 to May 2019,45 RIF patients(RIF group)and 30 normal pregnant women(normal pregnancy group)in reproductive health and gynecology department of Shenzhen Baoan Women’s and Children’s Hospital,Jinan University were selected as the research objects.The endometrial thickness,serum estradiol(E_(2))and progesterone(P)levels,and the expression of TERT,ER-αand PR in endometrium of the two groups were detected.Results:There was no significant difference in serum E_(2)and P levels between two groups during implantation window(P>0.05),but there was significant difference in endometrial thickness between RIF group and normal pregnancy group(P<0.05).TERT expression in endometrium of RIF group during implantation window was significantly higher than that of normal pregnancy group,while ER-αexpression was lower and PR expression was higher than those of normal pregnancy group(P<0.05).The expression of TERT was significantly correlated with the expression of ER-α(r=-0.6964,P<0.001)and PR(r=0.3471,P=0.0195).There was no significant difference in the value of E_(2)/P between the two groups,but compared with normal pregnancy group,the expression of ER-α/PR in endometrium of RIF group decreased during the implantation window,and the difference was statistically significant(P<0.001).Conclusion:Thin endometrium,unbalanced expression of ER-α,PR and increased expression of TERT in endometrium are the possible causes of implant failure.
作者
龙娜
孙汝亮
麦慧芬
李发科
张丽燕
郭宽萍
LONG Na;SUN Ruliang;MAI Huifen;LI Fake;ZHANG Liyan;GUO Kuanping(Department of Reproductive Health,Shenzhen Baoan Women’s and Children’s Hospital,Jinan University,Shenzhen 518100,China;Department of Pathology,Shenzhen Baoan Women’s and Children’s Hospital,Jinan University,Shenzhen 518100,China;Department of Gynaecology,Shenzhen Baoan Women’s and Children’s Hospital,Jinan University,Shenzhen 518100,China;Central Laboratory,Shenzhen Baoan Women’s and Children’s Hospital,Jinan University,Shenzhen 518100,China)
出处
《现代医学》
2021年第7期756-760,共5页
Modern Medical Journal
基金
深圳市宝安区科技计划项目(2016CX248)
深圳市科技创新委员会自然科学基金研究面上项目(JCYJ20190809144805528)
关键词
反复种植失败
种植窗
端粒酶催化亚基
雌激素受体Α
孕激素受体
recurrent implantation failure
implantation window
telomerase catalytic subunit
estrogen receptorα
progesterone receptor
