摘要
为探讨基于油水混溶剂的复方精油对空气中沉降菌的抑制作用,本研究测定了复方精油A的抑菌效果、精油型抑菌制剂的抑菌效果和油水混溶剂对精油抑菌效果的影响。通过平板沉降法测定复方精油A的抑菌效果,并选取PEG-40氢化蓖麻油(PEG-40)、吐温80、二甲基亚砜(DMSO)、司盘85、双脱乙醇5种油水混溶剂,在复方精油A和油水混溶剂比例分别介于1:1~1:6时配制精油抑菌制剂,并验证该均匀水溶液的抑菌效果,同时采用棋盘稀释法测定油水混溶剂对复方精油A抑菌效果的影响。结果表明,复方精油A的抑菌率达到80%以上,精油抑菌制剂在平板暴露6 h的抑菌率最佳,为48.92%。油水混溶剂中PEG-40的增溶效果最佳,复方精油浓度为1%时,精油、PEG-40、双脱乙醇的最佳比例为1:2:2;浓度为3%时,精油、PEG-40、双脱乙醇的最佳比例为1:3:3。PEG-40对空气中沉降菌无抑制效果,双脱乙醇和复方精油对空气中沉降菌的抑菌效果表现为相加作用。研究对植物精油应用于产业化生产时,油水混溶剂的种类和比例对精油类产品实际效果的影响有一定的参考价值。
To investigate the antibacterial effect of compound essential oil based on oil-water mixed solvent on settled bacteria in air, the antibacterial effect of the compound essential oil A and essential oil antibacterial preparations were studied, and the effect of oil-water mixed solvent on antibacterial effect of essential oil were determined. The inhibition effect of compound essential oil A was measured by plate sedimentation. Essential oil antibacterial preparations were made with the ratio of compound essential oil A and oil-water mixed solvent between 1:1 to 1:6, and five kinds of oil-water mixed solvents were chosen, such as PEG-40 hydrogenated castor oil, tween 80, dimethyl sulfoxide(DMSO), span 85 and double de-ethanol. Checkerboard dilution method was used to measure the inhibition effect of these preparations. And the results showed that, the bacteriostatic rate of compound essential oil A was larger than 80%, and the best bacteriostatic rate was48.92% when the essential oil antibacterial preparation has exposed on flat for six hours. When PEG-40 in oilwater mixed solvent got the best solubilization effect, the ratio of essential oil, PEG-40 and double-de-ethanol was 1:2:2 when the concentration of compound essential oil was 1%, and was 1:3:3 when the concentration was3%. PEG-40 showed no inhibition effect on settled bacteria in air, and the inhibition effect of double-deethanol and compound essential oil was additive. Based on the research above, when the plant essential oil is applied to industrial production, the type and proportion of the oil-water mixed solvent have certain actual effect on the essential oil product.
作者
马奕奕
王文翠
黄海燕
姚雷
Ma Yiyi;Wang Wencui;Huang Haiyan;Yao Lei(Shanghai Jiaotong University,Shanghai 200240;Hangzhou Blackgreen Workshop Trade Co.,Ltd,Hangzhou 310000)
出处
《中国农学通报》
2020年第7期135-142,共8页
Chinese Agricultural Science Bulletin
基金
杭州墨绿工坊贸易有限公司“净化空气精油产品开发研究”(18H2H22000047).
关键词
油水混溶剂
复方精油
沉降菌
精油抑菌制剂
抑菌效果
oil-water mixed solvent
compound essential oil
settling bacteria
essential oil antibacterial preparations
antibacterial effect
