Iron-based Prussian white(PW)materials have attracted considerable attention as promising cathodes for potassium-ion batteries(PIBs)due to their high capacity,easy preparation,and economic merits.However,the intrinsic...Iron-based Prussian white(PW)materials have attracted considerable attention as promising cathodes for potassium-ion batteries(PIBs)due to their high capacity,easy preparation,and economic merits.However,the intrinsic iron dissolution and uncontrollable cathode-electrolyte interface(CEI)formation in conventional organic electrolytes severely hinder their long-term cycling stability.Herein,we employ succinonitrile(SN),a bifunctional electrolyte additive,to suppress the iron dissolution and promote thin,uniform,and stable CEI formation of the PW cathode,thus improving its structural stability.Benefited from the coordination between the cyano groups in SN and iron atoms,this molecule can preferentially adsorb on the surface of PW to mitigate iron dissolution.SN also facilitates the decomposition of anions in potassium salt rather than organic solvents in electrolyte due to the attractive reaction between SN and anions.Consequently,the PW cathode with SN additive provides better electrochemical reversibility,showing capacity retention of 93.6%after 3000 cycles at 5C.In comparison,without SN,the capacity retention is only 87.4%after 1000 cycles under the same conditions.Moreover,the full cells of PW matched with commercial graphite(Gr)achieve stable cycling for 3500 cycles at a high rate of 20C,with an exceptional capacity decay of only 0.005%per cycle,surpassing the majority of recently reported results in literature.展开更多
The treatment of severe diabetic foot remains a clinical challenge.While it is established that bone can exert systemic effects through the secretion of osteokines on other organs,whether this endocrine function can b...The treatment of severe diabetic foot remains a clinical challenge.While it is established that bone can exert systemic effects through the secretion of osteokines on other organs,whether this endocrine function can be harnessed to promote diabetic wound healing remains unexplored.Here,we investigate the impact of a bone injury strategy on diabetic wound healing,leveraging the body’s innate regenerative capacity to stimulate osteokine release and influence remote skin wound repair.This study demonstrates that the tibial defect significantly accelerates ipsilateral diabetic foot skin wound healing.Mechanistically,we identify osteokines,platelet-derived growth factor-BB(PDGF-BB),as the key to initiating this process.Bone defect triggers a substantial release of PDGF-BB,which reaches the skin wound site via peripheral circulation.At the skin wound site,PDGF-BB mediates the secretion of keratinocyte growth factor(KGF)from fibroblasts via the PDGFRβsignaling pathway,thereby promoting the rapid re-epithelialization of epidermal cells through a paracrine pathway.Additionally,elevated PDGF-BB levels enhance the regeneration of CD31hi Emcnhi blood vessels within the wound.展开更多
BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can pre...BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can predict all-cause mortality and major adverse CV events(MACE)in patients with coronary artery disease(CAD)and to determine the prognostic value of adiponectin in predicting all-cause mortality and MACE in patients with stable CAD.METHODS We conducted a systematic search on PubMed,Scopus,and Google Scholar to find relevant studies published through June 2023 evaluating the long-term prognostic role of adiponectin in patients with stable CAD.Using a random effects model with 95%CI,we estimated the odds ratio(OR)while assessing heterogeneity through I^(2)statistics.To ensure robustness,we performed a sensitivity analysis using the leave-one-out approach.RESULTS After screening,we included five prospective studies involving 3225 patients who were followed up for a median duration of 3.8 years.Within the study population,prevalent risk factors included hypertension,diabetes,hyperlipidemia,and smoking.The commonly prescribed medications were angiotensin-converting enzyme inhibitors,beta blockers,and statins.The combined adjusted OR for all-cause mortality was found to be 2.51(95%CI:1.36–4.62),showing heterogeneity(I^(2)=65.51%,P=0.03).On the other hand,the combined adjusted OR for MACE was determined to be 1.04(95%CI:1.02–1.06)with no significant heterogeneity observed(I^(2)=0%,P=0.68).Through a sensitivity analysis,it was discovered that none of the studies significantly impacted the overall results of the meta-analysis,thus indicating their robustness.CONCLUSION Higher levels of adiponectin were found to be associated with an increased risk of long-term mortality and MACE in patients with CAD,which highlights its potential as a cost-effective marker for risk assessment and guiding treatment strategies.Further research on the role of adiponectin could greatly influence decision-making and resource allocation in CV care.展开更多
BACKGROUND Knowledge-based systems(KBS)are software applications based on a knowledge database and an inference engine.Various experimental KBS for computerassisted medical diagnosis and treatment were started to be u...BACKGROUND Knowledge-based systems(KBS)are software applications based on a knowledge database and an inference engine.Various experimental KBS for computerassisted medical diagnosis and treatment were started to be used since 70s(VisualDx,GIDEON,DXPlain,CADUCEUS,Internist-I,Mycin etc.).AIM To present in detail the“Electronic Pediatrician(EPed)”,a medical non-machine learning artificial intelligence(nml-AI)KBS in its prototype version created by the corresponding author(with database written in Romanian)that offers a physiopathology-based differential and positive diagnosis and treatment of ill children.METHODS EPed specifically focuses on the physiopathological reasoning of pediatric clinical cases.EPed has currently reached its prototype version 2.0,being able to diagnose 302 physiopathological macro-links(briefly named“clusters”)and 269 pediatric diseases:Some examples of diagnosis and a previous testing of EPed on a group of 34 patients are also presented in this paper.RESULTS The prototype EPed can currently diagnose 269 pediatric infectious and noninfectious diseases(based on 302 clusters),including the most frequent respiratory/digestive/renal/central nervous system infections,but also many other noninfectious pediatric diseases like autoimmune,oncological,genetical diseases and even intoxications,plus some important surgical pathologies.CONCLUSION EPed is the first and only physiopathology-based nml-AI KBS focused on general pediatrics and is the first and only pediatric Romanian KBS addressed to medical professionals.Furthermore,EPed is the first and only nml-AI KBS that offers not only both a physiopathology-based differential and positive disease diagnosis,but also identifies possible physiopathological“clusters”that may explain the signs and symptoms of any child-patient and may help treating that patient physiopathologically(until a final diagnosis is found),thus encouraging and developing the physiopathological reasoning of any clinician.展开更多
Decision support systems(DSS)based on physically based numerical models are standard tools used by water services and utilities.However,few DSS based on holistic approaches combining distributed hydrological,hydraulic...Decision support systems(DSS)based on physically based numerical models are standard tools used by water services and utilities.However,few DSS based on holistic approaches combining distributed hydrological,hydraulic,and hydrogeological models are operationally exploited.This holistic approach was adopted for the development of the AquaVar DSS,used for water resource management in the French Mediterranean Var watershed.The year 2019 marked the initial use of the DSS in its operational environment.Over the next 5 years,multiple hydrological events allowed to test the performance of the DSS.The results show that the tool is capable of simulating peak flows associated with two extreme rainfall events(storms Alex and Aline).For a moderate flood,the real-time functionality was able to simulate forecast discharges 26 h before the flood peak,with a maximum local error of 30%.Finally,simulations for the drought period 2022-2023 highlighted the essential need for DSS to evolve in line with changing climatic conditions,which give rise to unprecedented hydrological processes.The lessons learned from these first 5 years of AquaVar use under operational conditions are synthesized,addressing various topics such as DSS modularity,evolution,data positioning,technology,and governance.展开更多
BACKGROUND Steroids remain the primary treatment for severe alcohol-associated hepatitis(AAH),though there is little available tools to predict patient response to steroids.It was hypothesized that phosphatidylethanol...BACKGROUND Steroids remain the primary treatment for severe alcohol-associated hepatitis(AAH),though there is little available tools to predict patient response to steroids.It was hypothesized that phosphatidylethanol(PEth)value will inversely corre-late with response to steroid therapy based on Lille score in AAH.AIM To assess the relationship of patient factors,focusing on pre-steroid therapy PEth value,to steroid therapy response in AAH.METHODS A retrospective case control study was performed on patients who received≥4 days of steroid therapy for AAH at our hospital between July 1,2019 and June 30,2022.A total of 2087 patients were screened for AAH and those treated with steroids were included for statistical analysis utilizing independent sample t-test and for categorical variables using the χ^(2) test.RESULTS No correlation was found between PEth value,pre-steroids abstinence length,or number of drinks per week pre-steroids and response to steroids.Non-responder status significantly correlated with older age(P=0.024),lower albumin(P=0.003),and higher bilirubin(P=0.010)pre-steroids.Our study suggests that age,pre-steroid albumin,and pre-steroid bilirubin levels may predict nonresponse to steroid therapy.Non-responders have increased incidence of death and higher medical costs.CONCLUSION Identifying non-responders through these identified factors should prompt early referral for liver transplantation.Future prospective studies with larger population size are needed to assess the efficacy of combined pre-steroid age,albumin,bilirubin and other biochemical markers as predictors of steroid response.展开更多
BACKGROUND The use of intravascular ultrasound(iUS)has been shown in multiple singlecenter retrospective studies to decrease procedure time,radiation exposure,and needle passes compared to conventional fluoroscopic gu...BACKGROUND The use of intravascular ultrasound(iUS)has been shown in multiple singlecenter retrospective studies to decrease procedure time,radiation exposure,and needle passes compared to conventional fluoroscopic guidance in the creation of a transjugular intrahepatic portosystemic shunt(TIPS).However,there are few data regarding the impact of imaging guidance modality choice on clinical outcomes.AIM To determine the impact of iUS vs fluoroscopic guidance during creation of a TIPS on procedural metrics,liver injury,shunt patency and mortality.METHODS The retrospective study cohort consisted of 66 patients who underwent TIPS creation using iUS[“iUS-guided TIPS(iTIPS)group”]and 135 patients who underwent TIPS creation using fluoroscopic guidance[“conventional fluoroscopic-guided TIPS(cTIPS)group”]at 2 tertiary academic medical centers from 2015-2019.TIPS that required variceal embolization or portal vein recanalization were excluded.RESULTS The technical success rate was 100%in the iTIPS group and 96%in the cTIPS group(P=0.17).The iTIPS group had an air kerma(266±254 mGy vs 1235±1049 mGy,P<0.00001),dose area product(5728±6518 uGy×m^(2) vs 28969±19067 uGy×m^(2),P<0.00001),fluoroscopy time(18.7±9.6 minutes vs 32.3±19.0 minutes,P<0.00001),and total procedure time(93±40 minutes vs 110±51 minutes,P=0.01)which were significantly lower than the cTIPS group.There was no significant difference in liver function test adverse event grade at 1 month.With a median follow-up of 26 months(inter quartile range:6-61 months),there was no difference between the two groups in terms of thrombosis-free survival(P=0.23),intervention-free survival(P=0.29),or patient mortality(P=0.61).CONCLUSION The use of iUS in the creation of TIPS reduces radiation exposure and procedure time compared with fluoroscopic guidance.At midterm follow-up,the imaging guidance modality did not affect shunt patency or mortality.展开更多
BACKGROUND Parental presence in neonatal units(NUs)is essential for infant development and family well-being.A deeper understanding of the factors influencing parental presence is vital and will contribute to the deve...BACKGROUND Parental presence in neonatal units(NUs)is essential for infant development and family well-being.A deeper understanding of the factors influencing parental presence is vital and will contribute to the development of targeted interventions and policies that enhance parental engagement in neonatal care,thereby improving outcomes for infants and their families.AIM To identify and analyze primary factors influencing parental involvement in their child’s care in a neonatal intensive care unit(NICU).METHODS A literature search was conducted using the PubMed,MEDLINE,and Cochrane Library for systematic reviews databases,with the following search terms:“parental presence neonatology”,“couplet care”,“zero separation neonatal care”,“family integrated care”,“couplet care intervention”,“mother-child separation”,“parents newborn togetherness”,“mother-baby care”,“closeness and separation NICU”,“mother-infant interaction NICU”,“kangaroo care”,“dyad mother-infant”,and“newborn integrated care”.The database search for this literature review began on December 10,2024,with the final search conducted on April 10,2025.RESULTS The literature search yielded 281 articles,out of which 23 were selected for a detailed review.The factors associated with parental presence in NUs were grouped into five main categories:Parents’socio-demographic and cultural traits;the physical layout and care model of the NUs;the quality of parents’relationships with the healthcare staff;their active involvement in neonatal care;and the newborn’s health status.CONCLUSION The identification of factors that affect parental presence in NUs is critical for developing effective strategies aimed at encouraging increased parental involvement and ultimately improving neonatal and family outcomes.展开更多
The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate ne...The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.展开更多
Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribun...Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.展开更多
The rapid development of rare earth metal elements in the field of photocatalysis is due to their excellent optical and physicochemical properties.Benefiting from the unique external electronic structure of 4f_15d_16S...The rapid development of rare earth metal elements in the field of photocatalysis is due to their excellent optical and physicochemical properties.Benefiting from the unique external electronic structure of 4f_15d_16S_2,superior electronegativity of the 4f orbitals,and strong oxygen storage-release ability in Ce^(4+)/Ce^(3+)reversible pairs,cerium dioxide(CeO_(2))has attracted increasing interest from scientists.Nevertheless,the fast recombination of photoinduced electron-hole pairs and wide energy band gap of bare CeO_(2)significantly limit its practical applications.To overcome the above drawbacks,the construction of heterojunctions has been developed to broaden the absorption spectrum and accelerate the charge transfer.This review presents a mini-review of the synthesis of CeO_(2)-based heterojunctions including typeⅡ,Z-scheme,and S-scheme photocatalysts,as well as the corresponding applications in photocatalytic bactericidal and antitumor therapy.Finally,the latest advancements and potential perspectives on their future development are also discussed.展开更多
The Sine and Wormhole Energy Whale Optimization Algorithm(SWEWOA)represents an advanced solution method for resolving Optimal Power Flow(OPF)problems in power systems equipped with Flexible AC Transmission System(FACT...The Sine and Wormhole Energy Whale Optimization Algorithm(SWEWOA)represents an advanced solution method for resolving Optimal Power Flow(OPF)problems in power systems equipped with Flexible AC Transmission System(FACTS)devices which include Thyristor-Controlled Series Compensator(TCSC),Thyristor-Controlled Phase Shifter(TCPS),and Static Var Compensator(SVC).SWEWOA expands Whale Optimization Algorithm(WOA)through the integration of sine and wormhole energy features thus improving exploration and exploitation capabilities for efficient convergence in complex non-linear OPF problems.A performance evaluation of SWEWOA takes place on the IEEE-30 bus test system through static and dynamic loading scenarios where it demonstrates better results than five contemporary algorithms:Adaptive Chaotic WOA(ACWOA),WOA,Chaotic WOA(CWOA),Sine Cosine Algorithm Differential Evolution(SCADE),and Hybrid Grey Wolf Optimization(HGWO).The research shows that SWEWOA delivers superior generation cost reduction than other algorithms by reaching a minimum of 0.9%better performance.SWEWOA demonstrates superior power loss performance by achieving(P_(loss,min))at the lowest level compared to all other tested algorithms which leads to better system energy efficiency.The dynamic loading performance of SWEWOA leads to a 4.38%reduction in gross costs which proves its capability to handle different operating conditions.The algorithm achieves top performance in Friedman Rank Test(FRT)assessments through multiple performance metrics which verifies its consistent reliability and strong stability during changing power demands.The repeated simulations show that SWEWOA generates mean costs(C_(gen,min))and mean power loss values(P_(loss,min))with small deviations which indicate its capability to maintain cost-effective solutions in each simulation run.SWEWOA demonstrates great potential as an advanced optimization solution for power system operations through the results presented in this study.展开更多
Constructing new Brönsted acid sites within zeolitic materials holds paramount importance for the advancement of solid-acid catalysis.Zeo-type germanosilicates,a class of metallosilicates with a neutral framework...Constructing new Brönsted acid sites within zeolitic materials holds paramount importance for the advancement of solid-acid catalysis.Zeo-type germanosilicates,a class of metallosilicates with a neutral framework composed of tetravalent Ge and Si oxygen tetrahedrons,are conventionally considered not to generate Brönsted acid sites.Herein,we disclose an abnormal phenomenon with Ge-rich IWW-type germanosilicate(IWW-A)as an example that Ge-enriched germanosilicates are featured by mild Brönsted acidity.Using the art-of-state density functional theory calculation,19F magic angle spinning nuclear magnetic resonance,microcalorimetric and ammonia infrared mass spectrometry-temperature-programmed desorption characterizations,the nature of germanosilicate's Brönsted acidity has been demonstrated to be closely related to the neighboring framework Ge-hydroxyl pairs.Besides,the contribution of Ge-OH groups to Brönsted acidity and the role of Ge-pair structure for maintaining mild acid strength have been elucidated.In catalytic cracking of n-hexane and methanol-to-olefins reaction,the IWW-A germanosilicate exhibit high light olefins selectivity,good recyclability and low carbon deposition,outperforming the benchmark zeolite catalyst,ZSM-5 aluminosilicate.展开更多
After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact...After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact are not well understood.In this work,we aimed to study the correlation between angiogenesis and neurogenesis after a telencephalic stab wound injury.To this end,we used zebrafish as a relevant model of neuroplasticity and brain repair mechanisms.First,using the Tg(fli1:EGFP×mpeg1.1:mCherry)zebrafish line,which enables visualization of blood vessels and microglia respectively,we analyzed regenerative angiogenesis from 1 to 21 days post-lesion.In parallel,we monitored brain cell proliferation in neurogenic niches localized in the ventricular zone by using immunohistochemistry.We found that after brain damage,the blood vessel area and width as well as expression of the fli1 transgene and vascular endothelial growth factor(vegfaa and vegfbb)were increased.At the same time,neural stem cell proliferation was also increased,peaking between 3 and 5 days post-lesion in a manner similar to angiogenesis,along with the recruitment of microglia.Then,through pharmacological manipulation by injecting an anti-angiogenic drug(Tivozanib)or Vegf at the lesion site,we demonstrated that blocking or activating Vegf signaling modulated both angiogenic and neurogenic processes,as well as microglial recruitment.Finally,we showed that inhibition of microglia by clodronate-containing liposome injection or dexamethasone treatment impairs regenerative neurogenesis,as previously described,as well as injury-induced angiogenesis.In conclusion,we have described regenerative angiogenesis in zebrafish for the first time and have highlighted the role of inflammation in this process.In addition,we have shown that both angiogenesis and neurogenesis are involved in brain repair and that microglia and inflammation-dependent mechanisms activated by Vegf signaling are important contributors to these processes.This study paves the way for a better understanding of the effect of Vegf on microglia and for studies aimed at promoting angiogenesis to improve brain plasticity after brain injury.展开更多
GaN diodes for high energy(64.8 MeV)proton detection were fabricated and investigated.A comparison of the performance of GaN diodes with different structures is presented,with a focus on sapphire and on GaN substrates...GaN diodes for high energy(64.8 MeV)proton detection were fabricated and investigated.A comparison of the performance of GaN diodes with different structures is presented,with a focus on sapphire and on GaN substrates,Schottky and pin diodes,and different active layer thicknesses.Pin diodes fabricated on a sapphire substrate are the best choice for a GaN proton detector working at 0 V bias.They are sensitive(minimum detectable proton beam<1 pA/cm^(2)),linear as a function of proton current and fast(<1 s).High proton current sensitivity and high spatial resolution of GaN diodes can be exploited in the future for proton imaging of patients in proton therapy.展开更多
Soil organic carbon(SOC)plays a crucial role in mangrove blue carbon formation,yet the differences in microbemediated underlying SOC sequestration between introduced and native mangroves remain unclear.Here,we compare...Soil organic carbon(SOC)plays a crucial role in mangrove blue carbon formation,yet the differences in microbemediated underlying SOC sequestration between introduced and native mangroves remain unclear.Here,we compared the SOC pool,including recalcitrant organic carbon(ROC)and labile carbon pools,as well as three residual carbon sources(amino sugars,lignin phenols,and lipids)in sediments between mangroves of introduced Sonneratia apetala and native Kandelia obovata,and further connected them with microbial life strategies and C metabolism capability.The results showed that SOC accumulation in S.apetala(SA)sediment was about 30%-50% of that in K.obovata(KO)sediment.ROC was the dominant form of SOC in long-term sequestration(76%-83%),while lignin phenols,amino sugars,and lipids were important sources of ROC.In S.apetala sediments,the ROC content was positively correlated with amino sugars,resulting from the more r-strategist microbes that can rapidly convert plant-derived carbon into microbial biomass,which is subsequently transformed into microbial necromass.In contrast,in K.obovata sediments,ROC content showed a stronger positive correlation with the concentrations of lignin phenols and lipids.More K-strategist fungi in the topsoil of K.obovata increased enzyme activities,while more K-strategist bacteria in the subsoil enhanced carbon utilization capacity,thereby increasing lignin phenols and lipids from plant residues in both soil layers.Meanwhile,higher Ca^(2+)concentrations in K.obovata sediments protected three residual carbons from further microbe decomposition.This study provides valuable insights into the molecular mechanisms of SOC sequestration mediated by microbial life strategies in mangrove ecosystems.展开更多
Seed germination plays a pivotal role in plant growth and undergoes many intricate biochemical changes including lipid metabolism.Nevertheless,little is known about lipid changes and distributions in different structu...Seed germination plays a pivotal role in plant growth and undergoes many intricate biochemical changes including lipid metabolism.Nevertheless,little is known about lipid changes and distributions in different structures of soybean seeds during germination.Here,we applied mass spectrometry imaging(MSI)in conjunction with MS-based lipidomics to examine the lipid alterations in the embryo and cotyledon of soybean seeds during germination.To expand the coverage of lipid detection in soybean seeds,we used the novel techniques of matrix-assisted laser desorption/ionization(MALDI)and MALDI coupled with laser-postionization(MALDI-2).The results revealed that compared to MALDI,MALDI-2 enhanced the detected numbers and intensities of lipid species in various lipid classes,except for a few classes(e.g.,sphingomyelin and phosphatidylcholine).Lipidomic data showed that compared to the embryo,the cotyledon demonstrated slower but similar lipid changes during germination.These changes included the reduced levels of glycerolipids,phospholipids,and sterols,as well as the increased levels of lysophospholipids.Data from MALDI&MALDI-2 MSI supported and complemented these lipidomic findings.Our work highlights the significance of integrating lipid profiles and distributions to enhance our understanding of the metabolic pathways involved in seed germination.展开更多
A promising solution to combat harmful cyanobacterial blooms(CyanoHABs)is the use of naturally-derived cyanocidal compound.This study found that the crude extracts of the herbaceous plant Macleaya cordata can inhibit ...A promising solution to combat harmful cyanobacterial blooms(CyanoHABs)is the use of naturally-derived cyanocidal compound.This study found that the crude extracts of the herbaceous plant Macleaya cordata can inhibit the growth of the bloom-forming cyanobacterium Microcystis aeruginosa.Growth inhibition test suggested that the inhibitory effect was significant at a concentration as low as 5μg/L of the crude extracts.To comprehensively elucidate inhibitory mechanisms,we examined the responses in anti-oxidative systems,changes in triggering signals in cells,and hallmarks of programmed cell death(PCD)under the two levels of exposure to the crude extracts of M.cordata.A notable observation across all treatment groups was the significant increase in the superoxide dismutase activity of M.aeruginosa at 72 h.However,distinct patterns were observed in malondialdehyde(MDA),with no significant difference observed between the low concentration treatment group(5μg/L)and the control group.In contrast,Microcystis cells subjected to high concentration(10μg/L)exhibited a significant difference in MDA content at both 24 and 72 h.Furthermore,we observed that the levels of potential signaling molecules,e.g.,intracellular reactive oxygen species,nitric oxide,and Ca^(2+)were altered in the Microcystis cells after exposure to the crude extracts.This results in alterations to the levels of signaling molecules,which in turn stimulate the upregulation of orthocaspase,leading to PCD and population collapse eventually.This study examines the mechanisms of Microcystis suppression through naturally-derived substances,providing theoretical and technical foundations for the sustainable management of CyanoHABs.展开更多
The rapid expansion of Internet of Things(IoT)networks has introduced challenges in network management,primarily in maintaining energy efficiency and robust connectivity across an increasing array of devices.This pape...The rapid expansion of Internet of Things(IoT)networks has introduced challenges in network management,primarily in maintaining energy efficiency and robust connectivity across an increasing array of devices.This paper introduces the Adaptive Blended Marine Predators Algorithm(AB-MPA),a novel optimization technique designed to enhance Quality of Service(QoS)in IoT systems by dynamically optimizing network configurations for improved energy efficiency and stability.Our results represent significant improvements in network performance metrics such as energy consumption,throughput,and operational stability,indicating that AB-MPA effectively addresses the pressing needs ofmodern IoT environments.Nodes are initiated with 100 J of stored energy,and energy is consumed at 0.01 J per square meter in each node to emphasize energy-efficient networks.The algorithm also provides sufficient network lifetime extension to a resourceful 7000 cycles for up to 200 nodes with a maximum Packet Delivery Ratio(PDR)of 99% and a robust network throughput of up to 1800 kbps in more compact node configurations.This study proposes a viable solution to a critical problem and opens avenues for further research into scalable network management for diverse applications.展开更多
In this study,we employed Bayesian inversion coupled with the summation-by-parts and simultaneousapproximation-term(SBP-SAT)forward simulation method to elucidate the mechanisms behind mininginduced seismic events cau...In this study,we employed Bayesian inversion coupled with the summation-by-parts and simultaneousapproximation-term(SBP-SAT)forward simulation method to elucidate the mechanisms behind mininginduced seismic events caused by fault slip and their potential effects on rockbursts.Through Bayesian inversion,it is determined that the sources near fault FQ14 have a significant shear component.Additionally,we analyzed the stress and displacement fields of high-energy events,along with the hypocenter distribution of aftershocks,which aided in identifying the slip direction of the critically stressed fault FQ14.We also performed forward modeling to capture the complex dynamics of fault slip under varying friction laws and shear fracture modes.The selection of specific friction laws for fault slip models was based on their ability to accurately replicate observed slip behavior under various external loading conditions,thereby enhancing the applicability of our findings.Our results suggest that the slip behavior of fault FQ14 can be effectively understood by comparing different scenarios.展开更多
基金funding support from the Macao Science and Technology Development Fund(0013/2021/AMJ and 0082/2022/A2)support from the Multi-Year Research Grants(MYRG2022-00266-IAPME,and MYRG-GRG2023-00224-IAPME)provided by the Research&Development Office at the University of Macao+2 种基金the National Natural Science Foundation of China(52202328)the Shanghai Sailing Program(22YF1455500)the Shanghai Magnolia Talent Plan Pujiang Project(24PJD128)for their financial support。
文摘Iron-based Prussian white(PW)materials have attracted considerable attention as promising cathodes for potassium-ion batteries(PIBs)due to their high capacity,easy preparation,and economic merits.However,the intrinsic iron dissolution and uncontrollable cathode-electrolyte interface(CEI)formation in conventional organic electrolytes severely hinder their long-term cycling stability.Herein,we employ succinonitrile(SN),a bifunctional electrolyte additive,to suppress the iron dissolution and promote thin,uniform,and stable CEI formation of the PW cathode,thus improving its structural stability.Benefited from the coordination between the cyano groups in SN and iron atoms,this molecule can preferentially adsorb on the surface of PW to mitigate iron dissolution.SN also facilitates the decomposition of anions in potassium salt rather than organic solvents in electrolyte due to the attractive reaction between SN and anions.Consequently,the PW cathode with SN additive provides better electrochemical reversibility,showing capacity retention of 93.6%after 3000 cycles at 5C.In comparison,without SN,the capacity retention is only 87.4%after 1000 cycles under the same conditions.Moreover,the full cells of PW matched with commercial graphite(Gr)achieve stable cycling for 3500 cycles at a high rate of 20C,with an exceptional capacity decay of only 0.005%per cycle,surpassing the majority of recently reported results in literature.
基金supported by the Key Program of the National Natural Science Foundation of China grants 32230059the Basic Science Center Program T2288102+1 种基金the National Natural Science Foundation of China grants 32301123the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry JKVD1211002.
文摘The treatment of severe diabetic foot remains a clinical challenge.While it is established that bone can exert systemic effects through the secretion of osteokines on other organs,whether this endocrine function can be harnessed to promote diabetic wound healing remains unexplored.Here,we investigate the impact of a bone injury strategy on diabetic wound healing,leveraging the body’s innate regenerative capacity to stimulate osteokine release and influence remote skin wound repair.This study demonstrates that the tibial defect significantly accelerates ipsilateral diabetic foot skin wound healing.Mechanistically,we identify osteokines,platelet-derived growth factor-BB(PDGF-BB),as the key to initiating this process.Bone defect triggers a substantial release of PDGF-BB,which reaches the skin wound site via peripheral circulation.At the skin wound site,PDGF-BB mediates the secretion of keratinocyte growth factor(KGF)from fibroblasts via the PDGFRβsignaling pathway,thereby promoting the rapid re-epithelialization of epidermal cells through a paracrine pathway.Additionally,elevated PDGF-BB levels enhance the regeneration of CD31hi Emcnhi blood vessels within the wound.
文摘BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can predict all-cause mortality and major adverse CV events(MACE)in patients with coronary artery disease(CAD)and to determine the prognostic value of adiponectin in predicting all-cause mortality and MACE in patients with stable CAD.METHODS We conducted a systematic search on PubMed,Scopus,and Google Scholar to find relevant studies published through June 2023 evaluating the long-term prognostic role of adiponectin in patients with stable CAD.Using a random effects model with 95%CI,we estimated the odds ratio(OR)while assessing heterogeneity through I^(2)statistics.To ensure robustness,we performed a sensitivity analysis using the leave-one-out approach.RESULTS After screening,we included five prospective studies involving 3225 patients who were followed up for a median duration of 3.8 years.Within the study population,prevalent risk factors included hypertension,diabetes,hyperlipidemia,and smoking.The commonly prescribed medications were angiotensin-converting enzyme inhibitors,beta blockers,and statins.The combined adjusted OR for all-cause mortality was found to be 2.51(95%CI:1.36–4.62),showing heterogeneity(I^(2)=65.51%,P=0.03).On the other hand,the combined adjusted OR for MACE was determined to be 1.04(95%CI:1.02–1.06)with no significant heterogeneity observed(I^(2)=0%,P=0.68).Through a sensitivity analysis,it was discovered that none of the studies significantly impacted the overall results of the meta-analysis,thus indicating their robustness.CONCLUSION Higher levels of adiponectin were found to be associated with an increased risk of long-term mortality and MACE in patients with CAD,which highlights its potential as a cost-effective marker for risk assessment and guiding treatment strategies.Further research on the role of adiponectin could greatly influence decision-making and resource allocation in CV care.
文摘BACKGROUND Knowledge-based systems(KBS)are software applications based on a knowledge database and an inference engine.Various experimental KBS for computerassisted medical diagnosis and treatment were started to be used since 70s(VisualDx,GIDEON,DXPlain,CADUCEUS,Internist-I,Mycin etc.).AIM To present in detail the“Electronic Pediatrician(EPed)”,a medical non-machine learning artificial intelligence(nml-AI)KBS in its prototype version created by the corresponding author(with database written in Romanian)that offers a physiopathology-based differential and positive diagnosis and treatment of ill children.METHODS EPed specifically focuses on the physiopathological reasoning of pediatric clinical cases.EPed has currently reached its prototype version 2.0,being able to diagnose 302 physiopathological macro-links(briefly named“clusters”)and 269 pediatric diseases:Some examples of diagnosis and a previous testing of EPed on a group of 34 patients are also presented in this paper.RESULTS The prototype EPed can currently diagnose 269 pediatric infectious and noninfectious diseases(based on 302 clusters),including the most frequent respiratory/digestive/renal/central nervous system infections,but also many other noninfectious pediatric diseases like autoimmune,oncological,genetical diseases and even intoxications,plus some important surgical pathologies.CONCLUSION EPed is the first and only physiopathology-based nml-AI KBS focused on general pediatrics and is the first and only pediatric Romanian KBS addressed to medical professionals.Furthermore,EPed is the first and only nml-AI KBS that offers not only both a physiopathology-based differential and positive disease diagnosis,but also identifies possible physiopathological“clusters”that may explain the signs and symptoms of any child-patient and may help treating that patient physiopathologically(until a final diagnosis is found),thus encouraging and developing the physiopathological reasoning of any clinician.
文摘Decision support systems(DSS)based on physically based numerical models are standard tools used by water services and utilities.However,few DSS based on holistic approaches combining distributed hydrological,hydraulic,and hydrogeological models are operationally exploited.This holistic approach was adopted for the development of the AquaVar DSS,used for water resource management in the French Mediterranean Var watershed.The year 2019 marked the initial use of the DSS in its operational environment.Over the next 5 years,multiple hydrological events allowed to test the performance of the DSS.The results show that the tool is capable of simulating peak flows associated with two extreme rainfall events(storms Alex and Aline).For a moderate flood,the real-time functionality was able to simulate forecast discharges 26 h before the flood peak,with a maximum local error of 30%.Finally,simulations for the drought period 2022-2023 highlighted the essential need for DSS to evolve in line with changing climatic conditions,which give rise to unprecedented hydrological processes.The lessons learned from these first 5 years of AquaVar use under operational conditions are synthesized,addressing various topics such as DSS modularity,evolution,data positioning,technology,and governance.
文摘BACKGROUND Steroids remain the primary treatment for severe alcohol-associated hepatitis(AAH),though there is little available tools to predict patient response to steroids.It was hypothesized that phosphatidylethanol(PEth)value will inversely corre-late with response to steroid therapy based on Lille score in AAH.AIM To assess the relationship of patient factors,focusing on pre-steroid therapy PEth value,to steroid therapy response in AAH.METHODS A retrospective case control study was performed on patients who received≥4 days of steroid therapy for AAH at our hospital between July 1,2019 and June 30,2022.A total of 2087 patients were screened for AAH and those treated with steroids were included for statistical analysis utilizing independent sample t-test and for categorical variables using the χ^(2) test.RESULTS No correlation was found between PEth value,pre-steroids abstinence length,or number of drinks per week pre-steroids and response to steroids.Non-responder status significantly correlated with older age(P=0.024),lower albumin(P=0.003),and higher bilirubin(P=0.010)pre-steroids.Our study suggests that age,pre-steroid albumin,and pre-steroid bilirubin levels may predict nonresponse to steroid therapy.Non-responders have increased incidence of death and higher medical costs.CONCLUSION Identifying non-responders through these identified factors should prompt early referral for liver transplantation.Future prospective studies with larger population size are needed to assess the efficacy of combined pre-steroid age,albumin,bilirubin and other biochemical markers as predictors of steroid response.
文摘BACKGROUND The use of intravascular ultrasound(iUS)has been shown in multiple singlecenter retrospective studies to decrease procedure time,radiation exposure,and needle passes compared to conventional fluoroscopic guidance in the creation of a transjugular intrahepatic portosystemic shunt(TIPS).However,there are few data regarding the impact of imaging guidance modality choice on clinical outcomes.AIM To determine the impact of iUS vs fluoroscopic guidance during creation of a TIPS on procedural metrics,liver injury,shunt patency and mortality.METHODS The retrospective study cohort consisted of 66 patients who underwent TIPS creation using iUS[“iUS-guided TIPS(iTIPS)group”]and 135 patients who underwent TIPS creation using fluoroscopic guidance[“conventional fluoroscopic-guided TIPS(cTIPS)group”]at 2 tertiary academic medical centers from 2015-2019.TIPS that required variceal embolization or portal vein recanalization were excluded.RESULTS The technical success rate was 100%in the iTIPS group and 96%in the cTIPS group(P=0.17).The iTIPS group had an air kerma(266±254 mGy vs 1235±1049 mGy,P<0.00001),dose area product(5728±6518 uGy×m^(2) vs 28969±19067 uGy×m^(2),P<0.00001),fluoroscopy time(18.7±9.6 minutes vs 32.3±19.0 minutes,P<0.00001),and total procedure time(93±40 minutes vs 110±51 minutes,P=0.01)which were significantly lower than the cTIPS group.There was no significant difference in liver function test adverse event grade at 1 month.With a median follow-up of 26 months(inter quartile range:6-61 months),there was no difference between the two groups in terms of thrombosis-free survival(P=0.23),intervention-free survival(P=0.29),or patient mortality(P=0.61).CONCLUSION The use of iUS in the creation of TIPS reduces radiation exposure and procedure time compared with fluoroscopic guidance.At midterm follow-up,the imaging guidance modality did not affect shunt patency or mortality.
文摘BACKGROUND Parental presence in neonatal units(NUs)is essential for infant development and family well-being.A deeper understanding of the factors influencing parental presence is vital and will contribute to the development of targeted interventions and policies that enhance parental engagement in neonatal care,thereby improving outcomes for infants and their families.AIM To identify and analyze primary factors influencing parental involvement in their child’s care in a neonatal intensive care unit(NICU).METHODS A literature search was conducted using the PubMed,MEDLINE,and Cochrane Library for systematic reviews databases,with the following search terms:“parental presence neonatology”,“couplet care”,“zero separation neonatal care”,“family integrated care”,“couplet care intervention”,“mother-child separation”,“parents newborn togetherness”,“mother-baby care”,“closeness and separation NICU”,“mother-infant interaction NICU”,“kangaroo care”,“dyad mother-infant”,and“newborn integrated care”.The database search for this literature review began on December 10,2024,with the final search conducted on April 10,2025.RESULTS The literature search yielded 281 articles,out of which 23 were selected for a detailed review.The factors associated with parental presence in NUs were grouped into five main categories:Parents’socio-demographic and cultural traits;the physical layout and care model of the NUs;the quality of parents’relationships with the healthcare staff;their active involvement in neonatal care;and the newborn’s health status.CONCLUSION The identification of factors that affect parental presence in NUs is critical for developing effective strategies aimed at encouraging increased parental involvement and ultimately improving neonatal and family outcomes.
基金supported by the National Natural Science Foundation of China,Nos.82272171(to ZY),82271403(to XL),81941011(to XL),31971279(to ZY),31730030(to XL)the Natural Science Foundation of Beijing,No.7222004(to HD).
文摘The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after injury.However,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains unknown.In this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic injury.Our findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated neurons.Importantly,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain function.These findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.
基金supported by the 2024 scientific promotion program funded by Jeju National University.
文摘Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.
基金Project supported by the National Key Research and Development Program of China(2022YFB3504100,2021YFB3500600)Chunhui Project Foundation of the Education Department of China(202200554)。
文摘The rapid development of rare earth metal elements in the field of photocatalysis is due to their excellent optical and physicochemical properties.Benefiting from the unique external electronic structure of 4f_15d_16S_2,superior electronegativity of the 4f orbitals,and strong oxygen storage-release ability in Ce^(4+)/Ce^(3+)reversible pairs,cerium dioxide(CeO_(2))has attracted increasing interest from scientists.Nevertheless,the fast recombination of photoinduced electron-hole pairs and wide energy band gap of bare CeO_(2)significantly limit its practical applications.To overcome the above drawbacks,the construction of heterojunctions has been developed to broaden the absorption spectrum and accelerate the charge transfer.This review presents a mini-review of the synthesis of CeO_(2)-based heterojunctions including typeⅡ,Z-scheme,and S-scheme photocatalysts,as well as the corresponding applications in photocatalytic bactericidal and antitumor therapy.Finally,the latest advancements and potential perspectives on their future development are also discussed.
文摘The Sine and Wormhole Energy Whale Optimization Algorithm(SWEWOA)represents an advanced solution method for resolving Optimal Power Flow(OPF)problems in power systems equipped with Flexible AC Transmission System(FACTS)devices which include Thyristor-Controlled Series Compensator(TCSC),Thyristor-Controlled Phase Shifter(TCPS),and Static Var Compensator(SVC).SWEWOA expands Whale Optimization Algorithm(WOA)through the integration of sine and wormhole energy features thus improving exploration and exploitation capabilities for efficient convergence in complex non-linear OPF problems.A performance evaluation of SWEWOA takes place on the IEEE-30 bus test system through static and dynamic loading scenarios where it demonstrates better results than five contemporary algorithms:Adaptive Chaotic WOA(ACWOA),WOA,Chaotic WOA(CWOA),Sine Cosine Algorithm Differential Evolution(SCADE),and Hybrid Grey Wolf Optimization(HGWO).The research shows that SWEWOA delivers superior generation cost reduction than other algorithms by reaching a minimum of 0.9%better performance.SWEWOA demonstrates superior power loss performance by achieving(P_(loss,min))at the lowest level compared to all other tested algorithms which leads to better system energy efficiency.The dynamic loading performance of SWEWOA leads to a 4.38%reduction in gross costs which proves its capability to handle different operating conditions.The algorithm achieves top performance in Friedman Rank Test(FRT)assessments through multiple performance metrics which verifies its consistent reliability and strong stability during changing power demands.The repeated simulations show that SWEWOA generates mean costs(C_(gen,min))and mean power loss values(P_(loss,min))with small deviations which indicate its capability to maintain cost-effective solutions in each simulation run.SWEWOA demonstrates great potential as an advanced optimization solution for power system operations through the results presented in this study.
文摘Constructing new Brönsted acid sites within zeolitic materials holds paramount importance for the advancement of solid-acid catalysis.Zeo-type germanosilicates,a class of metallosilicates with a neutral framework composed of tetravalent Ge and Si oxygen tetrahedrons,are conventionally considered not to generate Brönsted acid sites.Herein,we disclose an abnormal phenomenon with Ge-rich IWW-type germanosilicate(IWW-A)as an example that Ge-enriched germanosilicates are featured by mild Brönsted acidity.Using the art-of-state density functional theory calculation,19F magic angle spinning nuclear magnetic resonance,microcalorimetric and ammonia infrared mass spectrometry-temperature-programmed desorption characterizations,the nature of germanosilicate's Brönsted acidity has been demonstrated to be closely related to the neighboring framework Ge-hydroxyl pairs.Besides,the contribution of Ge-OH groups to Brönsted acidity and the role of Ge-pair structure for maintaining mild acid strength have been elucidated.In catalytic cracking of n-hexane and methanol-to-olefins reaction,the IWW-A germanosilicate exhibit high light olefins selectivity,good recyclability and low carbon deposition,outperforming the benchmark zeolite catalyst,ZSM-5 aluminosilicate.
基金supported by European Regional Development Funds RE0022527 ZEBRATOX(EU-Région Réunion-French State national counterpart,to Nicolas Diotel and Jean-Loup Bascands).
文摘After brain damage,regenerative angiogenesis and neurogenesis have been shown to occur simultaneously in mammals,suggesting a close link between these processes.However,the mechanisms by which these processes interact are not well understood.In this work,we aimed to study the correlation between angiogenesis and neurogenesis after a telencephalic stab wound injury.To this end,we used zebrafish as a relevant model of neuroplasticity and brain repair mechanisms.First,using the Tg(fli1:EGFP×mpeg1.1:mCherry)zebrafish line,which enables visualization of blood vessels and microglia respectively,we analyzed regenerative angiogenesis from 1 to 21 days post-lesion.In parallel,we monitored brain cell proliferation in neurogenic niches localized in the ventricular zone by using immunohistochemistry.We found that after brain damage,the blood vessel area and width as well as expression of the fli1 transgene and vascular endothelial growth factor(vegfaa and vegfbb)were increased.At the same time,neural stem cell proliferation was also increased,peaking between 3 and 5 days post-lesion in a manner similar to angiogenesis,along with the recruitment of microglia.Then,through pharmacological manipulation by injecting an anti-angiogenic drug(Tivozanib)or Vegf at the lesion site,we demonstrated that blocking or activating Vegf signaling modulated both angiogenic and neurogenic processes,as well as microglial recruitment.Finally,we showed that inhibition of microglia by clodronate-containing liposome injection or dexamethasone treatment impairs regenerative neurogenesis,as previously described,as well as injury-induced angiogenesis.In conclusion,we have described regenerative angiogenesis in zebrafish for the first time and have highlighted the role of inflammation in this process.In addition,we have shown that both angiogenesis and neurogenesis are involved in brain repair and that microglia and inflammation-dependent mechanisms activated by Vegf signaling are important contributors to these processes.This study paves the way for a better understanding of the effect of Vegf on microglia and for studies aimed at promoting angiogenesis to improve brain plasticity after brain injury.
基金support from MATRIX(ANR-22-CE92-0047)with financial support from ITMI Cancer of Aviesan within the framework of the 2021−2030 Cancer Control Strategy,on funds administrated by INSERM through the project NECTAR.Matilde Siviero acknowledges funding from the French−German University/Saarbrücken(contract CDOC-06-2022).
文摘GaN diodes for high energy(64.8 MeV)proton detection were fabricated and investigated.A comparison of the performance of GaN diodes with different structures is presented,with a focus on sapphire and on GaN substrates,Schottky and pin diodes,and different active layer thicknesses.Pin diodes fabricated on a sapphire substrate are the best choice for a GaN proton detector working at 0 V bias.They are sensitive(minimum detectable proton beam<1 pA/cm^(2)),linear as a function of proton current and fast(<1 s).High proton current sensitivity and high spatial resolution of GaN diodes can be exploited in the future for proton imaging of patients in proton therapy.
基金supported by grants from the National Natural Science Foundation of China(Nos.42076117 and 32160051)the Guangdong Basic and Applied Basic Research Foundation(Nos.2023A1515012772,2024A1515011721,and 2024A1515012249).
文摘Soil organic carbon(SOC)plays a crucial role in mangrove blue carbon formation,yet the differences in microbemediated underlying SOC sequestration between introduced and native mangroves remain unclear.Here,we compared the SOC pool,including recalcitrant organic carbon(ROC)and labile carbon pools,as well as three residual carbon sources(amino sugars,lignin phenols,and lipids)in sediments between mangroves of introduced Sonneratia apetala and native Kandelia obovata,and further connected them with microbial life strategies and C metabolism capability.The results showed that SOC accumulation in S.apetala(SA)sediment was about 30%-50% of that in K.obovata(KO)sediment.ROC was the dominant form of SOC in long-term sequestration(76%-83%),while lignin phenols,amino sugars,and lipids were important sources of ROC.In S.apetala sediments,the ROC content was positively correlated with amino sugars,resulting from the more r-strategist microbes that can rapidly convert plant-derived carbon into microbial biomass,which is subsequently transformed into microbial necromass.In contrast,in K.obovata sediments,ROC content showed a stronger positive correlation with the concentrations of lignin phenols and lipids.More K-strategist fungi in the topsoil of K.obovata increased enzyme activities,while more K-strategist bacteria in the subsoil enhanced carbon utilization capacity,thereby increasing lignin phenols and lipids from plant residues in both soil layers.Meanwhile,higher Ca^(2+)concentrations in K.obovata sediments protected three residual carbons from further microbe decomposition.This study provides valuable insights into the molecular mechanisms of SOC sequestration mediated by microbial life strategies in mangrove ecosystems.
基金supported by National Natural Science Foundation of China(No.22036001)。
文摘Seed germination plays a pivotal role in plant growth and undergoes many intricate biochemical changes including lipid metabolism.Nevertheless,little is known about lipid changes and distributions in different structures of soybean seeds during germination.Here,we applied mass spectrometry imaging(MSI)in conjunction with MS-based lipidomics to examine the lipid alterations in the embryo and cotyledon of soybean seeds during germination.To expand the coverage of lipid detection in soybean seeds,we used the novel techniques of matrix-assisted laser desorption/ionization(MALDI)and MALDI coupled with laser-postionization(MALDI-2).The results revealed that compared to MALDI,MALDI-2 enhanced the detected numbers and intensities of lipid species in various lipid classes,except for a few classes(e.g.,sphingomyelin and phosphatidylcholine).Lipidomic data showed that compared to the embryo,the cotyledon demonstrated slower but similar lipid changes during germination.These changes included the reduced levels of glycerolipids,phospholipids,and sterols,as well as the increased levels of lysophospholipids.Data from MALDI&MALDI-2 MSI supported and complemented these lipidomic findings.Our work highlights the significance of integrating lipid profiles and distributions to enhance our understanding of the metabolic pathways involved in seed germination.
基金supported by Technology Innovation Guidance Program of Jiangxi Province“Science and Technology&Water Conservancy”Joint Plan(No.2022KSG01004)the National Natural Science Foundation of China(Nos.42107150,32061143029,and 42307100)Kunming Dianchi and Plateau Lakes Institute(No.KMZC2023-C3–01268-YNYG-0015)。
文摘A promising solution to combat harmful cyanobacterial blooms(CyanoHABs)is the use of naturally-derived cyanocidal compound.This study found that the crude extracts of the herbaceous plant Macleaya cordata can inhibit the growth of the bloom-forming cyanobacterium Microcystis aeruginosa.Growth inhibition test suggested that the inhibitory effect was significant at a concentration as low as 5μg/L of the crude extracts.To comprehensively elucidate inhibitory mechanisms,we examined the responses in anti-oxidative systems,changes in triggering signals in cells,and hallmarks of programmed cell death(PCD)under the two levels of exposure to the crude extracts of M.cordata.A notable observation across all treatment groups was the significant increase in the superoxide dismutase activity of M.aeruginosa at 72 h.However,distinct patterns were observed in malondialdehyde(MDA),with no significant difference observed between the low concentration treatment group(5μg/L)and the control group.In contrast,Microcystis cells subjected to high concentration(10μg/L)exhibited a significant difference in MDA content at both 24 and 72 h.Furthermore,we observed that the levels of potential signaling molecules,e.g.,intracellular reactive oxygen species,nitric oxide,and Ca^(2+)were altered in the Microcystis cells after exposure to the crude extracts.This results in alterations to the levels of signaling molecules,which in turn stimulate the upregulation of orthocaspase,leading to PCD and population collapse eventually.This study examines the mechanisms of Microcystis suppression through naturally-derived substances,providing theoretical and technical foundations for the sustainable management of CyanoHABs.
文摘The rapid expansion of Internet of Things(IoT)networks has introduced challenges in network management,primarily in maintaining energy efficiency and robust connectivity across an increasing array of devices.This paper introduces the Adaptive Blended Marine Predators Algorithm(AB-MPA),a novel optimization technique designed to enhance Quality of Service(QoS)in IoT systems by dynamically optimizing network configurations for improved energy efficiency and stability.Our results represent significant improvements in network performance metrics such as energy consumption,throughput,and operational stability,indicating that AB-MPA effectively addresses the pressing needs ofmodern IoT environments.Nodes are initiated with 100 J of stored energy,and energy is consumed at 0.01 J per square meter in each node to emphasize energy-efficient networks.The algorithm also provides sufficient network lifetime extension to a resourceful 7000 cycles for up to 200 nodes with a maximum Packet Delivery Ratio(PDR)of 99% and a robust network throughput of up to 1800 kbps in more compact node configurations.This study proposes a viable solution to a critical problem and opens avenues for further research into scalable network management for diverse applications.
基金the Graduate Innovation Program of China University of Mining and Technology,the Fundamental Research Funds for the Central Universities(Grant No.2023WLKXJ017)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX23_2776)the Shandong Energy Group(Grant No.SNKJ2022BJ03-R28)。
文摘In this study,we employed Bayesian inversion coupled with the summation-by-parts and simultaneousapproximation-term(SBP-SAT)forward simulation method to elucidate the mechanisms behind mininginduced seismic events caused by fault slip and their potential effects on rockbursts.Through Bayesian inversion,it is determined that the sources near fault FQ14 have a significant shear component.Additionally,we analyzed the stress and displacement fields of high-energy events,along with the hypocenter distribution of aftershocks,which aided in identifying the slip direction of the critically stressed fault FQ14.We also performed forward modeling to capture the complex dynamics of fault slip under varying friction laws and shear fracture modes.The selection of specific friction laws for fault slip models was based on their ability to accurately replicate observed slip behavior under various external loading conditions,thereby enhancing the applicability of our findings.Our results suggest that the slip behavior of fault FQ14 can be effectively understood by comparing different scenarios.
