Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between th...Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between the results of AO/P1 staining and sperm apoptosis. Methods A prospective study was carried out, 235 infertile couples remedied using traditional in vitro fertilization （IVF） were included. Semen collected from 235 patients were stained by fluorescence dye, AO and PL at the same time the spermatozoa apoptosis rate was calculated by using flow cytometry to detect the rate of Annexin V＋/ PI- . The result of fluorescence was divided into 3 groups as green （G）, yellow （Y） and red （R） according to the color of fluorescence. The correlation between the percent of the 3 colors and clinical outcomes and spermatozoa apoptosis rate was evaluated. Results Significant negative correlation was observed between the percentage of Y and fertilization rate （r= -0.42, P=0.04）, no significant correlation was observed between the percentage of G, R and fertilization rate. The percentage of G, Y, R was not significantly different between pregnant patients and non-pregnant patients, respectiw,ly, while the percentage of Y was significant different between miscarriage patients and liveborn patients （r=0.6L P=0.01） and no significant difference exist in the percentage of G and R between liveborn patients and miscarriage patients. There was a significant positive correlation between the percentage of Y and the rate of Annexin V＋/ PI （r=0.53, P=0.04）, and no significant correlation was shown between the percentage of G, R and the rate of Annexin V＋/ PI-.Conclusion The percentage of yellow group of double fluorescence （AO/PI） staining will affect the fertilization rate and the miscarriage rate, and this group of spermatozoa may be connected with the spermatozoa apoptosis.展开更多
Objective To analyze the effect of spermatozoa apoptosis on the clinical outcomes during in vitro Fertilization （IVF-ET）. Methods A prospective analysis was carried out, 519 infertile couples were divided into 3 gro...Objective To analyze the effect of spermatozoa apoptosis on the clinical outcomes during in vitro Fertilization （IVF-ET）. Methods A prospective analysis was carried out, 519 infertile couples were divided into 3 groups according to the clinical outcomes with IVF, including pregnancy and birth （liveborn group）, pregnancy but abortion （miscarriage group）, failure to pregnancy （failure group）. Spermatozoa collected from 519 male partners were processed through density gradient centrifugation （DGC） and swim-up and the apoptosis of the spermatozoa, in ejaculated/processed semen, were evaluated using flow cytometry by determining the levels of disruption of mitochondrial membrane potential （d-MMP） and activation of caspase-3（CP3）Results For ejaculated semen, apoptosis was significantly different among .4 groups individually, the result was as follows： liveborn group 〈 failure group 〈 miscarriage group （P〈0.05）. For processed semen, miscarriage had the highest apoptosis level （P〈0.05）, and there was no significant difference between liveborn group and failure group （P〉0.05）. Compared with the d-MMP, the activation of CP3, either in ejaculated or processed semen, showed more powerful predictive value for miscarriage other than liveborn and failure. The cutoff value of CP3 in ejaculated semen was 42.0%, with sensitivity： 0.931, specificity： 0.804.Conclusion The level of apoptosis of spermatozoa played a strongly impact on the clinical outcomes with IVF and the activation of CP3 in ejaculated semen possessed a high predictive value for miscarriage.展开更多
基金supported by a grant from STCSM (Subject No.:074207002)
文摘Objective To estimate the predictive value of double fluorescence [acridine orange （AO）/propidium iodide （PI）] staining results for fertilization rate and clinical outcomes and analyze the correlation between the results of AO/P1 staining and sperm apoptosis. Methods A prospective study was carried out, 235 infertile couples remedied using traditional in vitro fertilization （IVF） were included. Semen collected from 235 patients were stained by fluorescence dye, AO and PL at the same time the spermatozoa apoptosis rate was calculated by using flow cytometry to detect the rate of Annexin V＋/ PI- . The result of fluorescence was divided into 3 groups as green （G）, yellow （Y） and red （R） according to the color of fluorescence. The correlation between the percent of the 3 colors and clinical outcomes and spermatozoa apoptosis rate was evaluated. Results Significant negative correlation was observed between the percentage of Y and fertilization rate （r= -0.42, P=0.04）, no significant correlation was observed between the percentage of G, R and fertilization rate. The percentage of G, Y, R was not significantly different between pregnant patients and non-pregnant patients, respectiw,ly, while the percentage of Y was significant different between miscarriage patients and liveborn patients （r=0.6L P=0.01） and no significant difference exist in the percentage of G and R between liveborn patients and miscarriage patients. There was a significant positive correlation between the percentage of Y and the rate of Annexin V＋/ PI （r=0.53, P=0.04）, and no significant correlation was shown between the percentage of G, R and the rate of Annexin V＋/ PI-.Conclusion The percentage of yellow group of double fluorescence （AO/PI） staining will affect the fertilization rate and the miscarriage rate, and this group of spermatozoa may be connected with the spermatozoa apoptosis.
基金supported by a grant from STCSM(Subject No:074207002)supported by research funds with the approval of the Ethical Committee of the Faculty of Medicine,Jiaotong University,Shanghai,China
文摘Objective To analyze the effect of spermatozoa apoptosis on the clinical outcomes during in vitro Fertilization （IVF-ET）. Methods A prospective analysis was carried out, 519 infertile couples were divided into 3 groups according to the clinical outcomes with IVF, including pregnancy and birth （liveborn group）, pregnancy but abortion （miscarriage group）, failure to pregnancy （failure group）. Spermatozoa collected from 519 male partners were processed through density gradient centrifugation （DGC） and swim-up and the apoptosis of the spermatozoa, in ejaculated/processed semen, were evaluated using flow cytometry by determining the levels of disruption of mitochondrial membrane potential （d-MMP） and activation of caspase-3（CP3）Results For ejaculated semen, apoptosis was significantly different among .4 groups individually, the result was as follows： liveborn group 〈 failure group 〈 miscarriage group （P〈0.05）. For processed semen, miscarriage had the highest apoptosis level （P〈0.05）, and there was no significant difference between liveborn group and failure group （P〉0.05）. Compared with the d-MMP, the activation of CP3, either in ejaculated or processed semen, showed more powerful predictive value for miscarriage other than liveborn and failure. The cutoff value of CP3 in ejaculated semen was 42.0%, with sensitivity： 0.931, specificity： 0.804.Conclusion The level of apoptosis of spermatozoa played a strongly impact on the clinical outcomes with IVF and the activation of CP3 in ejaculated semen possessed a high predictive value for miscarriage.
