For veterinary medications administered per os, animal health companies strive to develop highly palatable dosage forms that are voluntarily accepted by animals to improve compliance and convenience. Achieving high pa...For veterinary medications administered per os, animal health companies strive to develop highly palatable dosage forms that are voluntarily accepted by animals to improve compliance and convenience. Achieving high palatability is often complex and difficult even without the presence of an active ingredient. This work compared acceptance and preference studies, as standardized methods are not established for informing formulation development or for more routine testing. Formulation development was followed by an acceptance study completed with laboratory Beagle dogs. One acceptance study and one preference study were completed in mixed breed dogs, also laboratory-housed, to gain wider representation of dog breed and age. Through these studies, we have evaluated both formulation parameters and palatability study conduct. In general, more complex palatants that have appealing taste, smell, and mouth feel enhance voluntary uptake. However, dosage forms that are too chewy may not be freely consumed even with complex palatants. The addition of aroma can entice dogs to prehend the tablet, as observed in one preference study. Preference studies in the veterinary pharmaceutical field identify the preferred first choice or first prehend, but not which product will be routinely voluntarily and fully consumed. Acceptance studies with cross-over treatment groups are used to quantify the full consumption of a dosage form when a dog is not given two choices at once. Since all dogs in acceptance studies are offered all treatment groups throughout the study, a comparison between degrees of consumption could suggest that one formulation might be preferred over another.展开更多
In Africa, sustainable livestock production must address food security, as well as animal disease concerns simultaneously in addition to social aspects. Livestock disease challenges seem to be increasing with one of t...In Africa, sustainable livestock production must address food security, as well as animal disease concerns simultaneously in addition to social aspects. Livestock disease challenges seem to be increasing with one of them being lack of good animal diagnostics services with notable emerging zoonotic diseases which if not correctly diagnosed in animals pose risks to humans. The major livestock hubs in sub-Sahara Africa (SSA) include Uganda, Ethiopia, Tanzania and Nigeria and utilization of diagnostic services by veterinarians in these countries could inform further action by different stakeholders in the SSA region. A cross sectional survey with veterinarians was conducted between January 2022 and February 2022 to determine the number of veterinarians who have submitted samples for veterinary diagnosis, laboratory utilization rates and challenges faced by veterinarians using veterinary labs in sub-Sahara Africa between the period of 2018 and 2022. Methods: This was a cross sectional study, where an online questionnaire was shared with veterinarians through their online social group networks in different countries and data was captured and analyzed. Results: 74% (64/87) of veterinarians reported having submitted a sample to a lab, Government labs were the most utilized at 54%, followed by private labs at 37% and the human health labs recorded 9% utilization rate. The most faced challenge by veterinarians was failing to get samples to the lab because the labs where far (52%) with the least challenge reported being failure to understand the lab results at 3%. Conclusion: Government labs were the most utilized in this period, however notable challenges like failing to get samples to the lab still exist. Veterinary diagnosis is still an underutilized service in sub-Sahara Africa and more measures need to be worked on in terms of sample logistics, capacity building and trainings of both lab personnel and veterinarians in interpretation of results to ensure improved utilization of veterinary diagnostics services.展开更多
This research paper explores the significance of the “A360 Bot Framework” in Automation 360 (A360) platform. A360 is Automation Anywhere’s cloud-based automation platform designed to make business processes more ef...This research paper explores the significance of the “A360 Bot Framework” in Automation 360 (A360) platform. A360 is Automation Anywhere’s cloud-based automation platform designed to make business processes more efficient. It’s known for its user-friendly interface, which allows both technical and non-technical users to use it effectively. Automation 360 is versatile, offering a range of tools to automate tasks, manage complex workflows, and integrate various applications. It empowers users to create customized solutions for their specific needs. Being cloud-based it ensures scalability, security, and real-time updates, making it a top choice in the fast-paced digital world. As demand for A360 rises, having a structured way to develop bots becomes crucial. The paper introduces the “A360 Bot Framework” as a guiding approach for bot developments. This framework ensures consistency and scalability, especially when working with both professional developers and non-technical users. It outlines key elements like setting up work folders, managing logs, dealing with errors, and ensuring secure bot execution. Ultimately, the “A360 Bot Framework” is presented as a foundational structure that enhances consistency, resiliency, and development efficiency. By following predefined practices and templates, bot developers can mitigate risks and streamline debugging processes. This framework accelerates the bot development lifecycle, allowing developers to focus on specific functionalities and value-added features. The research paper aims to provide insights into the benefits of adopting the A360 Bot Framework and its potential to revolutionize A360 bot development practices, leading to more efficient and effective automation solutions.展开更多
I present a solution that explores the use of A360 subtasks as a comparable concept to functions in programming. By leveraging subtasks as reusable and maintainable functions, users can efficiently develop customized ...I present a solution that explores the use of A360 subtasks as a comparable concept to functions in programming. By leveraging subtasks as reusable and maintainable functions, users can efficiently develop customized high-quality automation solutions. Additionally, the paper introduces the retry framework, which allows for the automatic retrying of subtasks in the event of system or unknown exceptions. This framework enhances efficiency and reduces the manual effort required to retrigger bots. The A360 Subtask and Retry Framework templates provide valuable assistance to both professional and citizen developers, improving code quality, maintainability, and the overall efficiency and resiliency of automation solutions.展开更多
The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competen...The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competence interchangeably. Oocyte quality can be defined as the overall health of an oocyte whereas oocyte competence refers to the ability of an oocyte to be fertilized and develop into a healthy embryo. Diminished oocyte quality is believed to be a result of alterations in oocyte growth and maturation processes that stem from several pelvic and systemic factors before and after oocyte retrieval. In this review, we focus on the morphological and nonmorphological markers of oocyte quality. Strict restrictions that limit the number of oocytes fertilized in various countries have triggered researchers around the world to come up with the most appropriate and noninvasive markers that enhance oocyte selection and optimize IVF outcomes. PubMed, Google Scholar, and the Cochrane Library were used to search for peer-reviewed, original articles about oocyte quality markers. The review was written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Morphological markers are commonly used, but they are subjective, and no single marker can be used exclusively to predict oocyte competence and subsequent embryonic development potential. Furthermore, transcriptomics of differentially expressed genes in cumulus cells and assessment of metabolomics and other contents of follicular fluid have shown greater precision. However, their specificity to the different quality determinants needs further research.展开更多
Acquired resistance to chemotherapy is a major limitation in clinical treatment for breast cancer.Accumulating evidence from in vitro,in vivo and clinical studies suggest that acquired chemoresistance is progressive,m...Acquired resistance to chemotherapy is a major limitation in clinical treatment for breast cancer.Accumulating evidence from in vitro,in vivo and clinical studies suggest that acquired chemoresistance is progressive,multifactorial and involve genetic and epigenetic aberrations.Among various mechanisms that contribute to chemoresistance,cellular reprogramming has extensively been implicated in breast cancer resistance lately.Cellular reprogramming events such as acquisition of epithelial to mesenchymal transition(EMT)and cancer stemness(CSCs)not only provide cancer cells with reversible phenotypic plasticity and survival advantage against cytotoxicity but also leads to aggressiveness,metastasis,clinical resistance,tumor recurrence and poor survival.The transient and reversible nature of cellular reprogramming processes and their controlled interaction with epigenetic regulatory complexes strongly support the involvement of dynamic epigenetic regulatory network in governing the cellular reprogramming and associated acquired chemoresistance.Further,epigenetic modulations are also gaining interest as promising interventions addressing the cancer cell reprogramming machinery to overcome acquired chemoresistance.This review discusses the previous reports and our recent findings that lead to current understanding of epigenetic dysregulation dictating the cellular reprogramming processes such as acquisition of EMT and CSCs phenotype and how they co-ordinate to establish acquired drug resistance in breast cancer.展开更多
The performance of antimicrobial susceptibility testing(AST)of bacteria and the interpretation of AST results for bacteria isolated from animals are complex tasks which must be performed using standard published metho...The performance of antimicrobial susceptibility testing(AST)of bacteria and the interpretation of AST results for bacteria isolated from animals are complex tasks which must be performed using standard published methodology and overseen by experts in clinical microbiology and in consultation with clinical pharmacologists.Otherwise,AST has significant potential for errors and mistakes.In this review,we provide guidance on how to correctly perform AST of bacteria isolated from animals and interpret the AST results.Particular emphasis is placed on the various approved or published methodologies for the different bacteria as well as the application of interpretive criteria,including clinical breakpoints and epidemiological cut-off values(ECVs/ECOFFs).Application of approved interpretive criteria and definitions of susceptible,susceptible dose-dependent,nonsusceptible,intermediate,and resistant for clinical breakpoints as well as wild-type and non-wildtype for ECVs,are explained and the difficulties resulting from the lack of approved clinical breakpoints for other bacteria,indications,and animal species is discussed.The requirement of quality controls in any AST approach is also emphasized.In addition,important parameters,often used in monitoring and surveillance studies,such as MIC50,MIC90,and testing range,are explained and criteria for the classification of bacteria as multidrug-resistant,extensively drug-resistant or pandrug-resistant are provided.Common mistakes are presented and the means to avoid them are described.To provide the most accurate AST,one must strictly adhere to approved standards or validated methodologies,like those of the Clinical and Laboratory Standards Institute or other internationally accepted AST documents and the detailed information provided therein.展开更多
文摘For veterinary medications administered per os, animal health companies strive to develop highly palatable dosage forms that are voluntarily accepted by animals to improve compliance and convenience. Achieving high palatability is often complex and difficult even without the presence of an active ingredient. This work compared acceptance and preference studies, as standardized methods are not established for informing formulation development or for more routine testing. Formulation development was followed by an acceptance study completed with laboratory Beagle dogs. One acceptance study and one preference study were completed in mixed breed dogs, also laboratory-housed, to gain wider representation of dog breed and age. Through these studies, we have evaluated both formulation parameters and palatability study conduct. In general, more complex palatants that have appealing taste, smell, and mouth feel enhance voluntary uptake. However, dosage forms that are too chewy may not be freely consumed even with complex palatants. The addition of aroma can entice dogs to prehend the tablet, as observed in one preference study. Preference studies in the veterinary pharmaceutical field identify the preferred first choice or first prehend, but not which product will be routinely voluntarily and fully consumed. Acceptance studies with cross-over treatment groups are used to quantify the full consumption of a dosage form when a dog is not given two choices at once. Since all dogs in acceptance studies are offered all treatment groups throughout the study, a comparison between degrees of consumption could suggest that one formulation might be preferred over another.
文摘In Africa, sustainable livestock production must address food security, as well as animal disease concerns simultaneously in addition to social aspects. Livestock disease challenges seem to be increasing with one of them being lack of good animal diagnostics services with notable emerging zoonotic diseases which if not correctly diagnosed in animals pose risks to humans. The major livestock hubs in sub-Sahara Africa (SSA) include Uganda, Ethiopia, Tanzania and Nigeria and utilization of diagnostic services by veterinarians in these countries could inform further action by different stakeholders in the SSA region. A cross sectional survey with veterinarians was conducted between January 2022 and February 2022 to determine the number of veterinarians who have submitted samples for veterinary diagnosis, laboratory utilization rates and challenges faced by veterinarians using veterinary labs in sub-Sahara Africa between the period of 2018 and 2022. Methods: This was a cross sectional study, where an online questionnaire was shared with veterinarians through their online social group networks in different countries and data was captured and analyzed. Results: 74% (64/87) of veterinarians reported having submitted a sample to a lab, Government labs were the most utilized at 54%, followed by private labs at 37% and the human health labs recorded 9% utilization rate. The most faced challenge by veterinarians was failing to get samples to the lab because the labs where far (52%) with the least challenge reported being failure to understand the lab results at 3%. Conclusion: Government labs were the most utilized in this period, however notable challenges like failing to get samples to the lab still exist. Veterinary diagnosis is still an underutilized service in sub-Sahara Africa and more measures need to be worked on in terms of sample logistics, capacity building and trainings of both lab personnel and veterinarians in interpretation of results to ensure improved utilization of veterinary diagnostics services.
文摘This research paper explores the significance of the “A360 Bot Framework” in Automation 360 (A360) platform. A360 is Automation Anywhere’s cloud-based automation platform designed to make business processes more efficient. It’s known for its user-friendly interface, which allows both technical and non-technical users to use it effectively. Automation 360 is versatile, offering a range of tools to automate tasks, manage complex workflows, and integrate various applications. It empowers users to create customized solutions for their specific needs. Being cloud-based it ensures scalability, security, and real-time updates, making it a top choice in the fast-paced digital world. As demand for A360 rises, having a structured way to develop bots becomes crucial. The paper introduces the “A360 Bot Framework” as a guiding approach for bot developments. This framework ensures consistency and scalability, especially when working with both professional developers and non-technical users. It outlines key elements like setting up work folders, managing logs, dealing with errors, and ensuring secure bot execution. Ultimately, the “A360 Bot Framework” is presented as a foundational structure that enhances consistency, resiliency, and development efficiency. By following predefined practices and templates, bot developers can mitigate risks and streamline debugging processes. This framework accelerates the bot development lifecycle, allowing developers to focus on specific functionalities and value-added features. The research paper aims to provide insights into the benefits of adopting the A360 Bot Framework and its potential to revolutionize A360 bot development practices, leading to more efficient and effective automation solutions.
文摘I present a solution that explores the use of A360 subtasks as a comparable concept to functions in programming. By leveraging subtasks as reusable and maintainable functions, users can efficiently develop customized high-quality automation solutions. Additionally, the paper introduces the retry framework, which allows for the automatic retrying of subtasks in the event of system or unknown exceptions. This framework enhances efficiency and reduces the manual effort required to retrigger bots. The A360 Subtask and Retry Framework templates provide valuable assistance to both professional and citizen developers, improving code quality, maintainability, and the overall efficiency and resiliency of automation solutions.
文摘The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competence interchangeably. Oocyte quality can be defined as the overall health of an oocyte whereas oocyte competence refers to the ability of an oocyte to be fertilized and develop into a healthy embryo. Diminished oocyte quality is believed to be a result of alterations in oocyte growth and maturation processes that stem from several pelvic and systemic factors before and after oocyte retrieval. In this review, we focus on the morphological and nonmorphological markers of oocyte quality. Strict restrictions that limit the number of oocytes fertilized in various countries have triggered researchers around the world to come up with the most appropriate and noninvasive markers that enhance oocyte selection and optimize IVF outcomes. PubMed, Google Scholar, and the Cochrane Library were used to search for peer-reviewed, original articles about oocyte quality markers. The review was written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Morphological markers are commonly used, but they are subjective, and no single marker can be used exclusively to predict oocyte competence and subsequent embryonic development potential. Furthermore, transcriptomics of differentially expressed genes in cumulus cells and assessment of metabolomics and other contents of follicular fluid have shown greater precision. However, their specificity to the different quality determinants needs further research.
文摘Acquired resistance to chemotherapy is a major limitation in clinical treatment for breast cancer.Accumulating evidence from in vitro,in vivo and clinical studies suggest that acquired chemoresistance is progressive,multifactorial and involve genetic and epigenetic aberrations.Among various mechanisms that contribute to chemoresistance,cellular reprogramming has extensively been implicated in breast cancer resistance lately.Cellular reprogramming events such as acquisition of epithelial to mesenchymal transition(EMT)and cancer stemness(CSCs)not only provide cancer cells with reversible phenotypic plasticity and survival advantage against cytotoxicity but also leads to aggressiveness,metastasis,clinical resistance,tumor recurrence and poor survival.The transient and reversible nature of cellular reprogramming processes and their controlled interaction with epigenetic regulatory complexes strongly support the involvement of dynamic epigenetic regulatory network in governing the cellular reprogramming and associated acquired chemoresistance.Further,epigenetic modulations are also gaining interest as promising interventions addressing the cancer cell reprogramming machinery to overcome acquired chemoresistance.This review discusses the previous reports and our recent findings that lead to current understanding of epigenetic dysregulation dictating the cellular reprogramming processes such as acquisition of EMT and CSCs phenotype and how they co-ordinate to establish acquired drug resistance in breast cancer.
文摘The performance of antimicrobial susceptibility testing(AST)of bacteria and the interpretation of AST results for bacteria isolated from animals are complex tasks which must be performed using standard published methodology and overseen by experts in clinical microbiology and in consultation with clinical pharmacologists.Otherwise,AST has significant potential for errors and mistakes.In this review,we provide guidance on how to correctly perform AST of bacteria isolated from animals and interpret the AST results.Particular emphasis is placed on the various approved or published methodologies for the different bacteria as well as the application of interpretive criteria,including clinical breakpoints and epidemiological cut-off values(ECVs/ECOFFs).Application of approved interpretive criteria and definitions of susceptible,susceptible dose-dependent,nonsusceptible,intermediate,and resistant for clinical breakpoints as well as wild-type and non-wildtype for ECVs,are explained and the difficulties resulting from the lack of approved clinical breakpoints for other bacteria,indications,and animal species is discussed.The requirement of quality controls in any AST approach is also emphasized.In addition,important parameters,often used in monitoring and surveillance studies,such as MIC50,MIC90,and testing range,are explained and criteria for the classification of bacteria as multidrug-resistant,extensively drug-resistant or pandrug-resistant are provided.Common mistakes are presented and the means to avoid them are described.To provide the most accurate AST,one must strictly adhere to approved standards or validated methodologies,like those of the Clinical and Laboratory Standards Institute or other internationally accepted AST documents and the detailed information provided therein.
