Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and...Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.展开更多
The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging...The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging technique that allows in vivo evaluation of the skin.Traditional RCM was wide-probe-based,which limited its application on uneven and covered skin.In this study,we report the development of a portable reflectance confocal microscope(PRCM)in which all components were assembled in a handheld shell.Although the size and weight of the PRCM were reduced based on the use of a microelectromechanical system,the resolution was kept at 0.91μm,and the field of view of the system was 343μm×532μm.When used in vivo,the PRCM was able to visualize cellular and nuclear morphology for both mouse and human skin.PRCM evaluations were then performed on wounds after topically applied mesenchymal stem cells(MSCs)or saline treatment.The PRCM allowed visualization of the formation of collagen bundles,re-epithelization from the wound edge to the wound bed,and hair follicle regeneration,which were consistent with histological evaluations.Therefore,we offer new insights into monitoring the effects of topically applied MSCs on the process of wound healing by using PRCM.This study illustrates that the newly developed PRCM represents a promising device for real-time,noninvasive monitoring of the dynamic process of wound healing,which demonstrates its potential to diagnose,monitor,or predict disease in clinical wound therapy.展开更多
A thermo-responsive rewritable plasmonic bio-memory chip has been successfully fabricated on an indium tin oxide(ITO)glass slide by assembling core-satellite gold nanoclusters with different size of gold nanoparticles...A thermo-responsive rewritable plasmonic bio-memory chip has been successfully fabricated on an indium tin oxide(ITO)glass slide by assembling core-satellite gold nanoclusters with different size of gold nanoparticles(AuNPs)using double-strand DNA(dsDNA)linker.And the prepared 70@DNA20@13 gold nanoclusters(AuNCs)exhibited more stable and greater photothermal conversion ability.With short time irradiation by 633 nm microbeam laser,every individual AuNCs could be excited and remove the satellite AuNPs on its surface.Especially,in the dissociation process of AuNCs with 3−5 satellite,its color would change from yellow to green,which showed more significant reduction in the red channel of the dark-field microscopy(DFM)images and could be defined to state“0”and“1”respectively.Besides,this plasmonic nano bio-memory could transform cyclically its state between 0 and 1 which exhibited excellent rewritable ability.展开更多
文摘Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system’s performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.
基金the National Key Research andDevelopment Program of China(No.2021YFA1101100)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807)+3 种基金the Major Innovative Research Team of Suzhou,China(No.ZXT2019007)Suzhou Institute of Biomedical Engineering and Technology(SIBET)Jilin City Science and Technology Cooperation Project(No.E0550104)Science and Technology Innovation Talents in Universities of Henan Province and Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.
文摘The process of wound healing is routinely evaluated by histological evaluation in the clinic,which may cause scarring and secondary injury.Reflectance confocal microscopy(RCM)represents a noninvasive,real-time imaging technique that allows in vivo evaluation of the skin.Traditional RCM was wide-probe-based,which limited its application on uneven and covered skin.In this study,we report the development of a portable reflectance confocal microscope(PRCM)in which all components were assembled in a handheld shell.Although the size and weight of the PRCM were reduced based on the use of a microelectromechanical system,the resolution was kept at 0.91μm,and the field of view of the system was 343μm×532μm.When used in vivo,the PRCM was able to visualize cellular and nuclear morphology for both mouse and human skin.PRCM evaluations were then performed on wounds after topically applied mesenchymal stem cells(MSCs)or saline treatment.The PRCM allowed visualization of the formation of collagen bundles,re-epithelization from the wound edge to the wound bed,and hair follicle regeneration,which were consistent with histological evaluations.Therefore,we offer new insights into monitoring the effects of topically applied MSCs on the process of wound healing by using PRCM.This study illustrates that the newly developed PRCM represents a promising device for real-time,noninvasive monitoring of the dynamic process of wound healing,which demonstrates its potential to diagnose,monitor,or predict disease in clinical wound therapy.
基金the National Natural Science Foundation of China(NSFC,Nos.22204081,62075103,and 22374081)Project of State Key Laboratory of Organic Electronics and Information Displays,Nanjing University of Posts and Telecommunications(No.20230112)+1 种基金Natural Science Foundation of Jiangsu Province(No.BK20211271)State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2210).
文摘A thermo-responsive rewritable plasmonic bio-memory chip has been successfully fabricated on an indium tin oxide(ITO)glass slide by assembling core-satellite gold nanoclusters with different size of gold nanoparticles(AuNPs)using double-strand DNA(dsDNA)linker.And the prepared 70@DNA20@13 gold nanoclusters(AuNCs)exhibited more stable and greater photothermal conversion ability.With short time irradiation by 633 nm microbeam laser,every individual AuNCs could be excited and remove the satellite AuNPs on its surface.Especially,in the dissociation process of AuNCs with 3−5 satellite,its color would change from yellow to green,which showed more significant reduction in the red channel of the dark-field microscopy(DFM)images and could be defined to state“0”and“1”respectively.Besides,this plasmonic nano bio-memory could transform cyclically its state between 0 and 1 which exhibited excellent rewritable ability.
