Early correction of childhood malocclusion is timely managing morphological,structural,and functional abnormalities at different dentomaxillofacial developmental stages.The selection of appropriate imaging examination...Early correction of childhood malocclusion is timely managing morphological,structural,and functional abnormalities at different dentomaxillofacial developmental stages.The selection of appropriate imaging examination and comprehensive radiological diagnosis and analysis play an important role in early correction of childhood malocclusion.This expert consensus is a collaborative effort by multidisciplinary experts in dentistry across the nation based on the current clinical evidence,aiming to provide general guidance on appropriate imaging examination selection,comprehensive and accurate imaging assessment for early orthodontic treatment patients.展开更多
OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular d...OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular docking.A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi(Radix Notoginseng)at different doses for 30 d.The alveolar bone structure was observed by micro-CT,the periodontal tissue structure was observed by hematoxylin-eosin staining,and the related proteins changes was detected by immunohistochemical staining.RESULTS:Sanqi(Radix Notoginseng)and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17(IL-17),tumor necrosis factor(TNF),and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways.The active compound quercetin had good binding activity with interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),matrix metallopeptidase 9(MMP9),tumor necrosis factorα(TNF-α),Jun proto-oncogene(JUN),and C-X-C motif chemokine ligand 8(CXCL8)in periodontitis.Compared with normal group,the distance from the cementoenamel junction(CEJ)to the alveolar bone(AB)was increased,alveolar bone absorption was obvious,the periodontal tissue structure was disorganized,and IL-6 and TNF-αwere upregulated in periodontitis group;meanwhile,the distance from CEJ to AB was significantly decreased,alveolar bone resorption was reduced,periodontal tissue structure was improved,the expression of IL-6,TNF-α,IL-17 and retinoid-ralated orphan receptorγt(RORγt)were decreased,Forkhead Box P3(FOXP3)and IL-10 were increased after Sanqi(Radix Notoginseng)treatment.CONCLUSIONS:Sanqi(Radix Notoginseng)improves the structure of alveolar bone and gum,and reduces inflammation;the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.展开更多
BACKGROUND Extraction of impacted third molars often leads to severe complications caused by damage to the inferior alveolar nerve(IAN).AIM To proposes a method for the partial grinding of an impacted mandibular third...BACKGROUND Extraction of impacted third molars often leads to severe complications caused by damage to the inferior alveolar nerve(IAN).AIM To proposes a method for the partial grinding of an impacted mandibular third molar(IMM3)near the IAN to prevent IAN injury during IMM3 extraction.METHODS Between January 1996 and March 2022,25 patients with IMM3 roots near the IAN were enrolled.The first stage of the operation consisted of grinding a major part of the IMM3 crown with a high-speed turbine dental drill to achieve sufficient space between the mandibular second molar and IMM3.After 6 months,when the root tips were observed to be away from the IAN on X-ray examination,the remaining part of the IMM3 was completely removed.RESULTS All IMM3s were extracted easily without symptoms of IAN injury after extraction.CONCLUSION Partial IMM3 grinding may be a good alternative treatment option to avoid IAN injury in high-risk cases.展开更多
PTH-related peptide(PTHr P) improves the bone marrow micro-environment to activate the bone-remodelling, but the coordinated regulation of PTHr P and transforming growth factor-β(TGFβ) signalling in TMJ-OA remains i...PTH-related peptide(PTHr P) improves the bone marrow micro-environment to activate the bone-remodelling, but the coordinated regulation of PTHr P and transforming growth factor-β(TGFβ) signalling in TMJ-OA remains incompletely understood. We used disordered occlusion to establish model animals that recapitulate the ordinary clinical aetiology of TMJ-OA. Immunohistochemical and histological analyses revealed condylar fibrocartilage degeneration in model animals following disordered occlusion. TMJ-OA model animals administered intermittent PTHr P(i PTH) exhibited significantly decreased condylar cartilage degeneration. Micro-CT,histomorphometry, and Western Blot analyses disclosed that i PTH promoted subchondral bone formation in the TMJ-OA model animals. In addition, i PTH increased the number of osterix(OSX)-positive cells and osteocalcin(OCN)-positive cells in the subchondral bone marrow cavity. However, the number of osteoclasts was also increased by i PTH, indicating that subchondral bone volume increase was mainly due to the i PTH-mediated increase in the bone-formation ability of condylar subchondral bone.In vitro, PTHr P treatment increased condylar subchondral bone marrow-derived mesenchymal stem cell(SMSC) osteoblastic differentiation potential and upregulated the gene and protein expression of key regulators of osteogenesis. Furthermore, we found that PTHr P-PTH1R signalling inhibits TGFβ signalling during osteoblastic differentiation. Collectively, these data suggested that i PTH improves OA lesions by enhancing osteoblastic differentiation in subchondral bone and suppressing aberrant active TGFβsignalling. These findings indicated that PTHr P, which targets the TGFβ signalling pathway, may be an effective biological reagent to prevent and treat TMJ-OA in the clinic.展开更多
Xenogenic organ transplantation has been considered the most promising strategy in providing possible substitutes with the physiological function of the failing organs as well as solving the problem of insufficient do...Xenogenic organ transplantation has been considered the most promising strategy in providing possible substitutes with the physiological function of the failing organs as well as solving the problem of insufficient donor sources.However,the xenograft,suffered from immune rejection and ischemia-reperfusion injury(IRI),causes massive reactive oxygen species(ROS)expression and the subsequent cell apoptosis,leading to the xenograft failure.Our previous study found a positive role of PPAR-γ in antiinflammation through its immunomodulation effects,which inspires us to apply PPAR-γ agonist rosiglitazone(RSG)to address survival issue of xenograft with the potential to eliminate the excessive ROS.In this study,xenogenic bioroot was constructed by wrapping the dental follicle cells(DFC)with porcine extracellular matrix(p ECM).The hydrogen peroxide(H_(2)O_(2))-induced DFC was pretreated with RSG to observe its protection on the damaged biological function.Immunoflourescence staining and transmission electron microscope were used to detect the intracellular ROS level.SD rat orthotopic transplantation model and superoxide dismutase 1(SOD1)knockout mice subcutaneous transplantation model were applied to explore the regenerative outcome of the xenograft.It showed that RSG pretreatment significantly reduced the adverse effects of H2O2on DFC with decreased intracellular ROS expression and alleviated mitochondrial damage.In vivo results confirmed RSG administration substantially enhanced the host’s antioxidant capacity with reduced osteoclasts formation and increased periodontal ligament-like tissue regeneration efficiency,maximumly maintaining the xenograft function.We considered that RSG preconditioning could preserve the biological properties of the transplanted stem cells under oxidative stress(OS)microenvironment and promote organ regeneration by attenuating the inflammatory reaction and OS injury.展开更多
The rejuvenation of bone tissue remains a formidable challenge for osteoporosis(OP)patients who suffer severe bone degeneration or structural deterioration.To reverse the bone loss associated with OP,a dual-enzyme cas...The rejuvenation of bone tissue remains a formidable challenge for osteoporosis(OP)patients who suffer severe bone degeneration or structural deterioration.To reverse the bone loss associated with OP,a dual-enzyme cascade system(PCF@EA)was engineered as a biomimetic engine to regulate bone metabolism imbalance.The bienzyme-driven system was constructed by integrating functionalized polymeric composite fibers with two mineralization-promoting hydrolases:recombinant human ectonucleotide pyrophosphatase/phosphodiesterase 1(rhENPP1)and recombinant human alkaline phosphatase(rhALP).The bienzyme-driven engine efficaciously navigates mitochondria-activated mineralization through an autophagic process,thereby promoting osteogenic differentiation,increasing intracellular inorganic phosphate(Pi),and facilitating calcium influx.Concurrently,metabolic regulation mediated by the bienzyme-driven engine involves the PI3K–Akt pathway,tricarboxylic acid(TCA)cycle and glycerophosphate metabolism,significantly down-regulating the mineralization inhibitor pyrophosphate(PPi)while accelerating the formation of phosphate-related metabolites.Moreover,the enzyme-loaded substrates inhibited bone resorption by reducing expression of osteoclast activity markers,including Trap and Cath-K.This bienzymatic cascade strategy has demonstrated efficacy in restoring bone homeostasis in osteoporotic rats,significantly improving defect maturation and catalyzing the recovery of bone mineral density from severe loss back to baseline levels.The unique features of the bienzyme-driven engine provide a promising approach for the therapeutic treatment of degenerative skeletal diseases.展开更多
基金supports by the National Natural Science Foundation of China(Nos.82201135)"2015"Cultivation Program for Reserve Talents for Academic Leaders of Nanjing Stomatological School,Medical School of Nanjing University(No.0223A204).
文摘Early correction of childhood malocclusion is timely managing morphological,structural,and functional abnormalities at different dentomaxillofacial developmental stages.The selection of appropriate imaging examination and comprehensive radiological diagnosis and analysis play an important role in early correction of childhood malocclusion.This expert consensus is a collaborative effort by multidisciplinary experts in dentistry across the nation based on the current clinical evidence,aiming to provide general guidance on appropriate imaging examination selection,comprehensive and accurate imaging assessment for early orthodontic treatment patients.
基金Scientific Research Fund of Education Department of Yunnan Province Project:Potential Targets and Molecular Mechanisms of Sanqi(Radix Notoginseng),an Active Component of Yunnan Baiyao,in the Treatment of Periodontitis(No.2022Y204)Special Project for The Selection of High-level Scientific and Technological Talents and Innovation Teams-technical Innovation Talents Training Object Project:Technical Innovation Personnel Training Object Project(202405AD350005)。
文摘OBJECTIVE:To investigate the effect and mechanism of Sanqi(Radix Notoginseng)in treating periodontitis.METHODS:The active components and periodontitis targets were analyzed through network pharmacology and molecular docking.A rat model of periodontitis was established and rats were treated by continuous intragastric administration of Sanqi(Radix Notoginseng)at different doses for 30 d.The alveolar bone structure was observed by micro-CT,the periodontal tissue structure was observed by hematoxylin-eosin staining,and the related proteins changes was detected by immunohistochemical staining.RESULTS:Sanqi(Radix Notoginseng)and periodontitis had a total of 96 coincident targets that were significantly enriched in the interleukin 17(IL-17),tumor necrosis factor(TNF),and advanced glycation endproducts and the receptor of advanced glycation endproducts signaling pathways.The active compound quercetin had good binding activity with interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),matrix metallopeptidase 9(MMP9),tumor necrosis factorα(TNF-α),Jun proto-oncogene(JUN),and C-X-C motif chemokine ligand 8(CXCL8)in periodontitis.Compared with normal group,the distance from the cementoenamel junction(CEJ)to the alveolar bone(AB)was increased,alveolar bone absorption was obvious,the periodontal tissue structure was disorganized,and IL-6 and TNF-αwere upregulated in periodontitis group;meanwhile,the distance from CEJ to AB was significantly decreased,alveolar bone resorption was reduced,periodontal tissue structure was improved,the expression of IL-6,TNF-α,IL-17 and retinoid-ralated orphan receptorγt(RORγt)were decreased,Forkhead Box P3(FOXP3)and IL-10 were increased after Sanqi(Radix Notoginseng)treatment.CONCLUSIONS:Sanqi(Radix Notoginseng)improves the structure of alveolar bone and gum,and reduces inflammation;the mechanism involve in inhibiting IL-17 signaling pathway to suppress Th17 and promote Treg cells differentiation.
文摘BACKGROUND Extraction of impacted third molars often leads to severe complications caused by damage to the inferior alveolar nerve(IAN).AIM To proposes a method for the partial grinding of an impacted mandibular third molar(IMM3)near the IAN to prevent IAN injury during IMM3 extraction.METHODS Between January 1996 and March 2022,25 patients with IMM3 roots near the IAN were enrolled.The first stage of the operation consisted of grinding a major part of the IMM3 crown with a high-speed turbine dental drill to achieve sufficient space between the mandibular second molar and IMM3.After 6 months,when the root tips were observed to be away from the IAN on X-ray examination,the remaining part of the IMM3 was completely removed.RESULTS All IMM3s were extracted easily without symptoms of IAN injury after extraction.CONCLUSION Partial IMM3 grinding may be a good alternative treatment option to avoid IAN injury in high-risk cases.
基金NSFC grant(82170921,81771033)the Department of Science and Technology of Sichuan Province(2016JQ0054)to L.Z.The Yunnan Provincial Department of Science and Technology-Kunming Medical University granted a basic research joint special fund project(202001AY070001-151)to J.Z.
文摘PTH-related peptide(PTHr P) improves the bone marrow micro-environment to activate the bone-remodelling, but the coordinated regulation of PTHr P and transforming growth factor-β(TGFβ) signalling in TMJ-OA remains incompletely understood. We used disordered occlusion to establish model animals that recapitulate the ordinary clinical aetiology of TMJ-OA. Immunohistochemical and histological analyses revealed condylar fibrocartilage degeneration in model animals following disordered occlusion. TMJ-OA model animals administered intermittent PTHr P(i PTH) exhibited significantly decreased condylar cartilage degeneration. Micro-CT,histomorphometry, and Western Blot analyses disclosed that i PTH promoted subchondral bone formation in the TMJ-OA model animals. In addition, i PTH increased the number of osterix(OSX)-positive cells and osteocalcin(OCN)-positive cells in the subchondral bone marrow cavity. However, the number of osteoclasts was also increased by i PTH, indicating that subchondral bone volume increase was mainly due to the i PTH-mediated increase in the bone-formation ability of condylar subchondral bone.In vitro, PTHr P treatment increased condylar subchondral bone marrow-derived mesenchymal stem cell(SMSC) osteoblastic differentiation potential and upregulated the gene and protein expression of key regulators of osteogenesis. Furthermore, we found that PTHr P-PTH1R signalling inhibits TGFβ signalling during osteoblastic differentiation. Collectively, these data suggested that i PTH improves OA lesions by enhancing osteoblastic differentiation in subchondral bone and suppressing aberrant active TGFβsignalling. These findings indicated that PTHr P, which targets the TGFβ signalling pathway, may be an effective biological reagent to prevent and treat TMJ-OA in the clinic.
基金supported by the Nature Science Foundation of China(31971281,32201073,82270958)Innovative Talents Program of Sichuan Province(2022JDRC0043)Research and Develop Program,West China Hospital of Stomatology Sichuan University(RD-03-202106)。
文摘Xenogenic organ transplantation has been considered the most promising strategy in providing possible substitutes with the physiological function of the failing organs as well as solving the problem of insufficient donor sources.However,the xenograft,suffered from immune rejection and ischemia-reperfusion injury(IRI),causes massive reactive oxygen species(ROS)expression and the subsequent cell apoptosis,leading to the xenograft failure.Our previous study found a positive role of PPAR-γ in antiinflammation through its immunomodulation effects,which inspires us to apply PPAR-γ agonist rosiglitazone(RSG)to address survival issue of xenograft with the potential to eliminate the excessive ROS.In this study,xenogenic bioroot was constructed by wrapping the dental follicle cells(DFC)with porcine extracellular matrix(p ECM).The hydrogen peroxide(H_(2)O_(2))-induced DFC was pretreated with RSG to observe its protection on the damaged biological function.Immunoflourescence staining and transmission electron microscope were used to detect the intracellular ROS level.SD rat orthotopic transplantation model and superoxide dismutase 1(SOD1)knockout mice subcutaneous transplantation model were applied to explore the regenerative outcome of the xenograft.It showed that RSG pretreatment significantly reduced the adverse effects of H2O2on DFC with decreased intracellular ROS expression and alleviated mitochondrial damage.In vivo results confirmed RSG administration substantially enhanced the host’s antioxidant capacity with reduced osteoclasts formation and increased periodontal ligament-like tissue regeneration efficiency,maximumly maintaining the xenograft function.We considered that RSG preconditioning could preserve the biological properties of the transplanted stem cells under oxidative stress(OS)microenvironment and promote organ regeneration by attenuating the inflammatory reaction and OS injury.
基金supported by the National Natural Science Foundation of China(No.32171338)Sichuan International Science and Technology Innovation Cooperation Project(No.2024YFHZ0308)the Major Science and Technology Projects in Yunnan Province(202302AA310038).
文摘The rejuvenation of bone tissue remains a formidable challenge for osteoporosis(OP)patients who suffer severe bone degeneration or structural deterioration.To reverse the bone loss associated with OP,a dual-enzyme cascade system(PCF@EA)was engineered as a biomimetic engine to regulate bone metabolism imbalance.The bienzyme-driven system was constructed by integrating functionalized polymeric composite fibers with two mineralization-promoting hydrolases:recombinant human ectonucleotide pyrophosphatase/phosphodiesterase 1(rhENPP1)and recombinant human alkaline phosphatase(rhALP).The bienzyme-driven engine efficaciously navigates mitochondria-activated mineralization through an autophagic process,thereby promoting osteogenic differentiation,increasing intracellular inorganic phosphate(Pi),and facilitating calcium influx.Concurrently,metabolic regulation mediated by the bienzyme-driven engine involves the PI3K–Akt pathway,tricarboxylic acid(TCA)cycle and glycerophosphate metabolism,significantly down-regulating the mineralization inhibitor pyrophosphate(PPi)while accelerating the formation of phosphate-related metabolites.Moreover,the enzyme-loaded substrates inhibited bone resorption by reducing expression of osteoclast activity markers,including Trap and Cath-K.This bienzymatic cascade strategy has demonstrated efficacy in restoring bone homeostasis in osteoporotic rats,significantly improving defect maturation and catalyzing the recovery of bone mineral density from severe loss back to baseline levels.The unique features of the bienzyme-driven engine provide a promising approach for the therapeutic treatment of degenerative skeletal diseases.
