Studying tiie urban landscape pattern plays a crucial role in scientific land use and management and in improving the urban ecological environment In this paper, AutoCAD, ArcGIS, Fragstats, and other software were u...Studying tiie urban landscape pattern plays a crucial role in scientific land use and management and in improving the urban ecological environment In this paper, AutoCAD, ArcGIS, Fragstats, and other software were used to analyse the data of the fourth phase of land use in the core atea of Yangling Demonstration Zone. The results showed that： ① in the core area, the percentage of construction land incteased from 18.22% to 61.72%, and the percentage of agricultufal land decreased from 58.36% to 11.14%. And the fafm land was fragmented, and traffic connectivily was strengthened. The afea of garden land was reduced from 251.89 hm2 to 50.38 hm^2, and the landscape metric of forest land showed an inverted V-shaped curve. ②The year 2009 in four phases witnessed the greatest landscape fragmentation, both Edge Density （ED） and Ingest Patch Index （LPI） increased, and human interference enhanced the overall landscape complexity. Measures were fotmulated in terms of deaf development goals, optimized allocation of land resoutces, effective protection of ecological ted lines, and definite ecological responsibility, so as to optimize the urban landscape pattern.展开更多
Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultura...Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultural reformation inside and outside the college and reform of off-campus teaching experiment farm in view of outstanding agricultural characteristics of the bioengineering branch, with environmental health preservation as the fundamental goal and "health-preserving agriculture" as the link. In addition, four major characteristic landscape frameworks of "point, line, surface, and body" were established, and the effective construction approaches of health-preserving environmental cultural landscapes in vocational colleges were explored.展开更多
[Objectives]To evaluate the pollution status of phthalate esters(PAEs)in the soil of facility vegetable base in Yangling District of Xianyang City.[Methods]A total of 15 kinds of PAEs in soil samples were detected and...[Objectives]To evaluate the pollution status of phthalate esters(PAEs)in the soil of facility vegetable base in Yangling District of Xianyang City.[Methods]A total of 15 kinds of PAEs in soil samples were detected and analyzed by gas chromatography-mass spectrometry.[Results]A total of 12 kinds of PAEs were detected in analyzed soil samples,with a total content of 53.4-3524.1μg/kg and an average of 602.7μg/kg.Specifically,DEHP,DBP,DIBP,DMEP,BBP and DNOP were the main PAEs pollutants in the soil,with the detection rates of 100%,100%,100%,73.3%,63.3%and 53.3%,and the average content were 286.3,167.3,123.1,157.6,121.3,and 130.5μg/kg,respectively.[Conclusions]Compared with the soil in other regions,the pollution level of PAEs in the soil of facility vegetable base in Yangling District is lower,but such compounds are widespread in the facility vegetable base,and their potential environmental risks should attract close attention.展开更多
[Objectives]To evaluate the pollution status of antibiotics in the soil of vegetable bases in Yangling District.[Methods]The contents of 19 antibiotics in 4 categories of quinolones,tetracyclines,sulfonamides and macr...[Objectives]To evaluate the pollution status of antibiotics in the soil of vegetable bases in Yangling District.[Methods]The contents of 19 antibiotics in 4 categories of quinolones,tetracyclines,sulfonamides and macrolides in soil samples from 20 bases were detected and analyzed using HPLC-MS/MS.[Results]The quinolones,sulfonamides and tetracyclines were all detected in 100%of soil samples,and the detection rate of macrolides was 62%.The average contents of the four antibiotics from high to low were quinolones(51.76μg/kg)>tetracyclines(12.77μg/kg)>sulfonamides(1.14μg/kg)>macrolides(0.28μg/kg),and norfloxacin,oxytetracycline,sulfamethazine and erythromycin were the main contents,respectively.According to the trigger value of ecological toxic effect of antibiotics in soil(100μg/kg)proposed by the Steering Committee of the International Coordinating Committee for Veterinary Medicine(VICH),the contents of the four classes of antibiotics in the soil of the study area were all lower than the trigger value,indicating a small ecological risk.[Conclusions]Compared with other domestic research reports,the vegetable bases in this study area are also at a relatively low level of antibiotic pollution.展开更多
Zanthoxylum bungeanum is an economically important crop worldwide due to its high content of aroma-producing monoterpenoids,and development of varieties with enhanced flavor and overall quality is a crucial research a...Zanthoxylum bungeanum is an economically important crop worldwide due to its high content of aroma-producing monoterpenoids,and development of varieties with enhanced flavor and overall quality is a crucial research area.However,the transcriptional regulatory mechanisms underlying monoterpenoid synthesis in Z.bungeanum remain unclear,hindering these breeding efforts.In this study,RNA sequencing,gas chromatography–mass spectrometry,and other molecular biology techniques were used to identify the underlying transcriptional regulation mechanisms.Two transcription factors,ZbbHLH2 and ZbERF6,were identified as key regulators of monoterpenoid synthesis in Z.bungeanum that upregulate various monoterpenoid synthesis-associated genes and are novel transcriptional activators of ZbIDI,which encodes the rate-limiting enzyme in plant monoterpenoid synthesis.Functional analysis revealed that the expression of three genes[1]modulates monoterpenoid accumulation in Z.bungeanum peel.These findings provide novel insights into the metabolic regulatory network of monoterpenoid synthesis in Z.bungeanum peel,offer potential strategies for the biofortification of specific monoterpenoids,and will promote the development of Z.bungeanum germplasm for targeted breeding and quality improvement.展开更多
[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the ...[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.展开更多
[Objective]Leaf diseases significantly affect both the yield and quality of tea throughout the year.To address the issue of inadequate segmentation finesse in the current tea spot segmentation models,a novel diagnosis...[Objective]Leaf diseases significantly affect both the yield and quality of tea throughout the year.To address the issue of inadequate segmentation finesse in the current tea spot segmentation models,a novel diagnosis of the severity of tea spots was proposed in this research,designated as MDC-U-Net3+,to enhance segmentation accuracy on the base framework of U-Net3+.[Methods]Multi-scale feature fusion module(MSFFM)was incorporated into the backbone network of U-Net3+to obtain feature information across multiple receptive fields of diseased spots,thereby reducing the loss of features within the encoder.Dual multi-scale attention(DMSA)was incorporated into the skip connection process to mitigate the segmentation boundary ambiguity issue.This integration facilitates the comprehensive fusion of fine-grained and coarse-grained semantic information at full scale.Furthermore,the segmented mask image was subjected to conditional random fields(CRF)to enhance the optimization of the segmentation results[Results and Discussions]The improved model MDC-U-Net3+achieved a mean pixel accuracy(mPA)of 94.92%,accompanied by a mean Intersection over Union(mIoU)ratio of 90.9%.When compared to the mPA and mIoU of U-Net3+,MDC-U-Net3+model showed improvements of 1.85 and 2.12 percentage points,respectively.These results illustrated a more effective segmentation performance than that achieved by other classical semantic segmentation models.[Conclusions]The methodology presented herein could provide data support for automated disease detection and precise medication,consequently reducing the losses associated with tea diseases.展开更多
Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development...Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.展开更多
Although both abscisic acid(ABA)and methyl jasmonate(MeJA)play significant roles in regulating the development and quality of grape(Vitis vinifera L.)berries,the regulatory effects and mechanisms of the combined appli...Although both abscisic acid(ABA)and methyl jasmonate(MeJA)play significant roles in regulating the development and quality of grape(Vitis vinifera L.)berries,the regulatory effects and mechanisms of the combined application of ABA and MeJA remain unclear.To further explore the optimal combination of these hormones for regulating the development of grape quality,combined ABA and MeJA treatments were carried out in this study,with‘Jumeigui’grape used as the material.The results indicated that the combined treatment of high-ABA and low-MeJA(HA+LM)increased the sugar-acid ratio,promoted the accumulation of phenolic substances in grape skins,and resulted in anthocyanin content 168.9%higher than that of the control,significantly enhancing coloration.Additionally,the combined treatment of low-ABA and low-MeJA(LA+LM)was more conducive to the accumulation of phenols in grape,especially phenolic acid and resveratrol,as the total phenolic content increased by 38.96%relative to that of the control.Moreover,the expressions of aroma-related genes were upregulated by the combined high-MeJA treatments.The combined treatment of high-ABA and high-MeJA(HA+HM)markedly increased terpene biosynthesis,followed by the LA+HM treatment,increasing the intensity of the rose flavor characteristics of the‘Jumeigui’grape.Therefore,the combination of MeJA and ABA at different concentrations had distinct effects on fruit quality and appropriate combinations can be selected according to the specific needs for the targeted metabolites.展开更多
The Grassland Ecological Compensation Policy(GECP)is a large-scale project that has been investing in China since 2011.Despite the significant investment and long duration of the GECP,its impact on carbon sequestratio...The Grassland Ecological Compensation Policy(GECP)is a large-scale project that has been investing in China since 2011.Despite the significant investment and long duration of the GECP,its impact on carbon sequestration in grasslands remains unclear.Based on panel data from prefecture-level cities in the Yellow River Basin(YRB)from 2000 to 2020,this study explored the effects of the GECP on grassland carbon sequestration in the YRB and its heterogeneity using a time-varying difference-in-differences(TV-DID)model.Subsequently,we predicted changes in carbon sequestration in grasslands under the influence of the GECP from 2021 to 2040 using a simulation.The main conclusions are as follows:First,the implementation of the GECP promoted carbon sequestration in grasslands.This conclusion was validated after conducting a series of robustness tests.Moreover,the effects of the GECP were heterogeneous across different geographical locations,highway densities,and grassland areas.Finally,the simulation results indicated that over the next 20 years,grassland carbon sequestration will generally increase but exhibit cyclical changes.The government should align the principles and objectives of the GECP with local geographic conditions,resource availability,and socio-economic development.It must formulate and implement tailored policies that maximize the ecological protection benefits of the GECP and promote carbon sequestration in the grasslands of the YRB.展开更多
In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions....In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.展开更多
Proanthocyanidins(PAs)are a group of oligomeric flavonoids also known as condensed tannins.PAs in human diet have antioxidant and health-promoting effects.BSISTER(BS)promotes the accumulation of PAs,but its direct tar...Proanthocyanidins(PAs)are a group of oligomeric flavonoids also known as condensed tannins.PAs in human diet have antioxidant and health-promoting effects.BSISTER(BS)promotes the accumulation of PAs,but its direct target gene and regulation mechanism in PAs biosynthesis are not clear.Here,we reported VviBS1 and VviBS2 genes,which bind directly to the promoter of PA synthesis key genes VviANR(ANTHOCYANIDIN REDUCTASE)and VviLAR(LEUCOANTHOCYANIDIN REDUCTASE)and up-regulate their expression to promote the PAs biosynthesis in grapevine.VviBS1 and VviBS2 partially rescued the transparent testa phenotype of an Arabidopsis tt16 mutant.Overexpression of BS1 and BS2 in grapevine callus increased the content of PAs,as well as the expression of specific genes related to PA synthesis.VviBS1 and VviBS2 proteins bound directly to the promoter regions of the key PA synthesis genes VviANR1,VviANR2,and VviLAR1.Overexpression of VviBS1 and VviBS2 promotes VviANR1,VviANR2 and VviLAR1 expression and PAs biosynthesis.Some BS1-and BS2-interacting MADS-box proteins have an effect on PAs biosynthesis.This study provides insight into the regulatory mechanisms of PAs biosynthesis in grapevine,which could be effectively employed for metabolic engineering to increase PA content.展开更多
Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenes...Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenesis efficiency through allosteric regulation,redox state,and signal transduction pathways.However,the liver-enriched metabolites that regulate hepatic gluconeogenesis in dairy cows and their specific regulatory mechanisms remain incompletely characterized.Results Six Holstein dairy cows and six Duroc×(Landrace×Yorkshire)(DLY)crossbred pigs served as research subjects.Employing non-targeted and targeted metabolomics,we discovered that three bile acids—taurodeoxycholic acid(TDCA),taurocholic acid(TCA),and glycocholic acid(GCA)—were highly enriched in Holstein dairy cows'livers.In bovine hepatocytes,individual or combined stimulation of these bile acids significantly upregulated the expression of gluconeogenesis genes(FBP1,PCK1 and G6PC)and enhanced glucose production.In fasting mice with induced gluconeogenesis,TDCA,TCA,and GCA increased fasting blood glucose levels,and pyruvate tolerance tests further revealed their capacity to enhance hepatic gluconeogenesis,enabling more efficient glucose synthesis from pyruvate.Mechanistically,these bile acids activated Takeda G protein-coupled receptor 5(TGR5),elevated intracellular cAMP levels,and ultimately enhanced gluconeogenesis via the transcription factor cAMP-response element binding protein(CREB).Notably,a TGR5 inhibitor abrogated the stimulatory effects of TDCA,TCA,and GCA on hepatic gluconeogenesis in fasting mice.Conclusion TDCA,TCA,and GCA are key metabolites promoting hepatic gluconeogenesis in dairy cows,with TGR5 as the pivotal receptor and the cAMP/PKA/CREB pathway as the critical downstream mechanism.展开更多
Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to bioti...Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.展开更多
Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hor...Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hormone derived from carotenoid,strigolactone(SL)is produced in the roots of plants.It was first reported that SL can induce seed germination of root-parasitic plants.In recent years,it has been shown that strigolactone plays a regulatory role in plant response to abiotic stresses.By eliminating oxidative stress caused by reactive oxygen species,it can potentially increase photosynthetic rate,chlorophyll content,and thus enhance plant drought resistance.Transcriptome studies have explored signal transduction,antioxidant enzyme activity,transcription factors,and expression of stress-and metabolism-related genes induced by extrinsic strigolactone in plants,the effects of strigolactone on plant growth and development have been preliminarily determined,but the studies on inducing crop tolerance to abiotic stresses are still unknown.In this review,the physiological and molecular aspects of the induction of the response to stress in horticultural crops by strigolactone were reviewed.It is important to improve the tolerance and productivity of horticultural crops under abiotic stress.展开更多
Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon&...Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.展开更多
Background The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention.Taurochenodeoxycholic acid(TCDCA)is the predominant bile acid within the chicke...Background The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention.Taurochenodeoxycholic acid(TCDCA)is the predominant bile acid within the chicken bile acid pool and is closely related to metabolic disorders.The current study aims to investigate the potential effects of TCDCA on abdominal fat deposition in broilers.From 14 to 28 days of age,the broilers in the CON group received an oral administration of 1 mL of saline,while those in the treatment groups were administered 1 mL of a solution containing 0.05 g,0.10 g,or 0.20 g of TCDCA.Results The results showed that TCDCA treatments from 14 to 28 d had no significant effects on BW,ADFI,ADG and FCR in broilers at the age of 28 days of age.However,the abdominal fat percentage in the 0.20 g TCDCA group significantly increased,accompanied by higher TBA and HDL-c levels,as well as a reduction in apolipoprotein B levels in serum.In addition,serum triglyceride levels tended to be higher in the 0.20 g TCDCA group(P=0.098).The 0.20 g TCDCA treatment increased the gene expressions of SREBP-1,C/EBP-α,and ELOVL6,while decreasing the mRNA abundance of ATGL and CPT-1 in the abdominal fat.Serum levels of TCDCA,TDCA,and THDCA were significantly higher after 0.20 g TCDCA administration,while TCA levels were significantly lower,as determined by the targeted bile acid metabolomics analysis.Conversely,hepatic mRNA levels of CYP7A1,CYP27A1,BAAT,and BSEP were increased in the 0.20 g TCDCA group.The oral administration of 0.20 g TCDCA also upregulated the expression of FXR,VDR,and FGF19 in abdominal fat.The 16S rRNA analysis of cecal microbiota revealed that a decrease in the Shannon and Simpson indexes in the 0.20 g TCDCA group,and an increase in the Firmicutes/Bacteroidetes ratio.LEfSe analysis revealed that the predominant bacteria in the CON group were Streptococcus and Oscillospira at the genus level,while Lactobacillus,Parabacteroides,Anaeroplasma,and Helicobacter were identified as the dominant genera in the 0.20 g TCDCA group.Functional predictions for the gut microbiota exhibited that lipid metabolism,replication and repair pathway were enhanced in the 0.20 g TCDCA group.Correlation analysis demonstrated that the abundance of Lactobacillus was positively correlated with serum levels of TCDCA,THDCA,and TDCA,while the abundance of Streptococcus and Oscillospira showed a positive correlation with serum TCA levels.Conclusion Overall,this study elucidates that the intervention of 0.20 g TCDCA may promote abdominal fat deposition by activating bile acid receptors in abdominal fat,and concurrent alterations in both the intestinal microbial community and bile acid profile.展开更多
Desert shrubs are indispensable in maintaining ecological stability by reducing soil erosion,enhancing water retention,and boosting soil fertility,which are critical factors in mitigating desertification processes.Due...Desert shrubs are indispensable in maintaining ecological stability by reducing soil erosion,enhancing water retention,and boosting soil fertility,which are critical factors in mitigating desertification processes.Due to the complex topography,variable climate,and challenges in field surveys in desert regions,this paper proposes YOLO-Desert-Shrub(YOLO-DS),a detection method for identifying desert shrubs in UAV remote sensing images based on an enhanced YOLOv8n framework.This method accurately identifying shrub species,locations,and coverage.To address the issue of small individual plants dominating the dataset,the SPDconv convolution module is introduced in the Backbone and Neck layers of the YOLOv8n model,replacing conventional convolutions.This structural optimization mitigates information degradation in fine-grained data while strengthening discriminative feature capture across spatial scales within desert shrub datasets.Furthermore,a structured state-space model is integrated into the main network,and the MambaLayer is designed to dynamically extract and refine shrub-specific features from remote sensing images,effectively filtering out background noise and irrelevant interference to enhance feature representation.Benchmark evaluations reveal the YOLO-DS framework attains 79.56%mAP40weight,demonstrating 2.2%absolute gain versus the baseline YOLOv8n architecture,with statistically significant advantages over contemporary detectors in cross-validation trials.The predicted plant coverage exhibits strong consistency with manually measured coverage,with a coefficient of determination(R^(2))of 0.9148 and a Root Mean Square Error(RMSE)of1.8266%.The proposed UAV-based remote sensing method utilizing the YOLO-DS effectively identify and locate desert shrubs,monitor canopy sizes and distribution,and provide technical support for automated desert shrub monitoring.展开更多
Anthocyanins are biosynthesized in the endoplasmic reticulum and stored in vacuoles,and glutathione S-transferase(GST)plays a key role in anthocyanin accumulation.However,little is known about the mechanism of the rol...Anthocyanins are biosynthesized in the endoplasmic reticulum and stored in vacuoles,and glutathione S-transferase(GST)plays a key role in anthocyanin accumulation.However,little is known about the mechanism of the role of GST in this process.We identified a prominent candidate gene,MaGST1,which is highly expressed during the full-bloom stage,in grape hyacinth.MaGST1 rescued the phenotype of the anthocyanin biosynthesis-deficient mutant tt19,indicating that MaGST1 functions in anthocyanin transport.MaGST1-RNAi transgenic petals were generated by stable genetic transformation.We found that MaGST1-RNAi inhibited the accumulation of anthocyanin in petals.Interestingly,in vitro experiments revealed that recombinant MaGST1 increased the water solubilities of cyanidin(Cya),delphinidin 3-O-glucoside(Dp3G),and petunidin 3-O-glucoside(Pt3G).Compared with Dp3G and Pt3G,Cya significantly suppressed the quenching of the intrinsic tryptophan fluorescence of MaGST1 to a lower level,indicating that MaGST1 has a greater affinity for Cya than for Dp3G and Pt3G.Site-directed mutagenesis and anthocyanin water solubility assays revealed that MaGST1 primarily binds to anthocyanin via Ser-68.Furthermore,yeast one-hybrid,dual-luciferase,and GUS staining assays revealed that MaGST1 expression is positively regulated by MaMybA.In summary,our findings reveal the molecular mechanism of anthocyanin transport mediated by MaGST1.展开更多
The irrigation districts of northern China face issues such as water scarcity,inability to effectively utilize flood resources,and groundwater overexploitation.In view of these challenges,this study proposes a new con...The irrigation districts of northern China face issues such as water scarcity,inability to effectively utilize flood resources,and groundwater overexploitation.In view of these challenges,this study proposes a new concept of deep storage irrigation through flood resources utilization.However,whether deep storage irrigation can recharge deep soil moisture and sustain crop production still requires further study.A two-year field experiment was conducted on summer maize in the Guanzhong Plain with five soil wetting layer depths(T1:60 cm;T2:90 cm;T3:120 cm;T4:150 cm;T5:180 cm)and soil saturation moisture content as the irrigation upper limit.The results presented that the ranges of deep soil moisture recharge in the100–200 cm soil profile(SMS_(100–200))was 73.34–267.42 and 0–150.03 mm in 2021(wet season)and 2022(normal season).When the effective precipitation and irrigation exceeded 390 mm,the SMS_(100–200)began to linearly increase.The highest grain yield(GY)were observed at T2 and T3 treatments in 2021(11.44 t ha^(-1))and 2022(11.25 t ha^(-1)),respectively.The maize GY of T4 in 2021 and T5 in 2022 were only 3.9 and 5.7%lower than the maximize GY,respectively.However,the SMS_(100–200)for T4 and T5 were 2.4 and 5.0 times that of T2 and T3 treatments in 2021 and 2022,respectively.Overall,the further increase in irrigation amounts induced only a slight decrease in grain yield,but it significantly increased deep soil moisture recharge.Therefore,the deep storage irrigation breaks through the traditional idea of water-saving irrigation with limited water resources,which can be utilized as an effective alternative to address the issues of water scarcity,low flood resources utilization,and groundwater level declines in the irrigation districts of northern China.展开更多
基金Sponsored by Humanities and Social Sciences Project in Northwest A&F University(2015RWYB38)
文摘Studying tiie urban landscape pattern plays a crucial role in scientific land use and management and in improving the urban ecological environment In this paper, AutoCAD, ArcGIS, Fragstats, and other software were used to analyse the data of the fourth phase of land use in the core atea of Yangling Demonstration Zone. The results showed that： ① in the core area, the percentage of construction land incteased from 18.22% to 61.72%, and the percentage of agricultufal land decreased from 58.36% to 11.14%. And the fafm land was fragmented, and traffic connectivily was strengthened. The afea of garden land was reduced from 251.89 hm2 to 50.38 hm^2, and the landscape metric of forest land showed an inverted V-shaped curve. ②The year 2009 in four phases witnessed the greatest landscape fragmentation, both Edge Density （ED） and Ingest Patch Index （LPI） increased, and human interference enhanced the overall landscape complexity. Measures were fotmulated in terms of deaf development goals, optimized allocation of land resoutces, effective protection of ecological ted lines, and definite ecological responsibility, so as to optimize the urban landscape pattern.
基金Sponsored by Humanities and Social Sciences Research Foundation of Yangling Vocational&Technical College(GJ1423)
文摘Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultural reformation inside and outside the college and reform of off-campus teaching experiment farm in view of outstanding agricultural characteristics of the bioengineering branch, with environmental health preservation as the fundamental goal and "health-preserving agriculture" as the link. In addition, four major characteristic landscape frameworks of "point, line, surface, and body" were established, and the effective construction approaches of health-preserving environmental cultural landscapes in vocational colleges were explored.
基金Supported by Scientific Research Fund Project of Yangling Vocational and Technical College(A2018053)Science and Technology Plan Project of Yangling Agricultural High-tech Industry Demonstration Zone(2018SF-02).
文摘[Objectives]To evaluate the pollution status of phthalate esters(PAEs)in the soil of facility vegetable base in Yangling District of Xianyang City.[Methods]A total of 15 kinds of PAEs in soil samples were detected and analyzed by gas chromatography-mass spectrometry.[Results]A total of 12 kinds of PAEs were detected in analyzed soil samples,with a total content of 53.4-3524.1μg/kg and an average of 602.7μg/kg.Specifically,DEHP,DBP,DIBP,DMEP,BBP and DNOP were the main PAEs pollutants in the soil,with the detection rates of 100%,100%,100%,73.3%,63.3%and 53.3%,and the average content were 286.3,167.3,123.1,157.6,121.3,and 130.5μg/kg,respectively.[Conclusions]Compared with the soil in other regions,the pollution level of PAEs in the soil of facility vegetable base in Yangling District is lower,but such compounds are widespread in the facility vegetable base,and their potential environmental risks should attract close attention.
基金Supported by Science and Technology Plan Project of Yangling Demonstration Zone(2018SF-02)Scientific Researchof Yangling Vocational and Technical College(A2018053)。
文摘[Objectives]To evaluate the pollution status of antibiotics in the soil of vegetable bases in Yangling District.[Methods]The contents of 19 antibiotics in 4 categories of quinolones,tetracyclines,sulfonamides and macrolides in soil samples from 20 bases were detected and analyzed using HPLC-MS/MS.[Results]The quinolones,sulfonamides and tetracyclines were all detected in 100%of soil samples,and the detection rate of macrolides was 62%.The average contents of the four antibiotics from high to low were quinolones(51.76μg/kg)>tetracyclines(12.77μg/kg)>sulfonamides(1.14μg/kg)>macrolides(0.28μg/kg),and norfloxacin,oxytetracycline,sulfamethazine and erythromycin were the main contents,respectively.According to the trigger value of ecological toxic effect of antibiotics in soil(100μg/kg)proposed by the Steering Committee of the International Coordinating Committee for Veterinary Medicine(VICH),the contents of the four classes of antibiotics in the soil of the study area were all lower than the trigger value,indicating a small ecological risk.[Conclusions]Compared with other domestic research reports,the vegetable bases in this study area are also at a relatively low level of antibiotic pollution.
基金supported by the National Natural Science Foundation of China(31872706)the National Key Research and Development Program of China(2019 YFD1000603).
文摘Zanthoxylum bungeanum is an economically important crop worldwide due to its high content of aroma-producing monoterpenoids,and development of varieties with enhanced flavor and overall quality is a crucial research area.However,the transcriptional regulatory mechanisms underlying monoterpenoid synthesis in Z.bungeanum remain unclear,hindering these breeding efforts.In this study,RNA sequencing,gas chromatography–mass spectrometry,and other molecular biology techniques were used to identify the underlying transcriptional regulation mechanisms.Two transcription factors,ZbbHLH2 and ZbERF6,were identified as key regulators of monoterpenoid synthesis in Z.bungeanum that upregulate various monoterpenoid synthesis-associated genes and are novel transcriptional activators of ZbIDI,which encodes the rate-limiting enzyme in plant monoterpenoid synthesis.Functional analysis revealed that the expression of three genes[1]modulates monoterpenoid accumulation in Z.bungeanum peel.These findings provide novel insights into the metabolic regulatory network of monoterpenoid synthesis in Z.bungeanum peel,offer potential strategies for the biofortification of specific monoterpenoids,and will promote the development of Z.bungeanum germplasm for targeted breeding and quality improvement.
文摘[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.
文摘[Objective]Leaf diseases significantly affect both the yield and quality of tea throughout the year.To address the issue of inadequate segmentation finesse in the current tea spot segmentation models,a novel diagnosis of the severity of tea spots was proposed in this research,designated as MDC-U-Net3+,to enhance segmentation accuracy on the base framework of U-Net3+.[Methods]Multi-scale feature fusion module(MSFFM)was incorporated into the backbone network of U-Net3+to obtain feature information across multiple receptive fields of diseased spots,thereby reducing the loss of features within the encoder.Dual multi-scale attention(DMSA)was incorporated into the skip connection process to mitigate the segmentation boundary ambiguity issue.This integration facilitates the comprehensive fusion of fine-grained and coarse-grained semantic information at full scale.Furthermore,the segmented mask image was subjected to conditional random fields(CRF)to enhance the optimization of the segmentation results[Results and Discussions]The improved model MDC-U-Net3+achieved a mean pixel accuracy(mPA)of 94.92%,accompanied by a mean Intersection over Union(mIoU)ratio of 90.9%.When compared to the mPA and mIoU of U-Net3+,MDC-U-Net3+model showed improvements of 1.85 and 2.12 percentage points,respectively.These results illustrated a more effective segmentation performance than that achieved by other classical semantic segmentation models.[Conclusions]The methodology presented herein could provide data support for automated disease detection and precise medication,consequently reducing the losses associated with tea diseases.
基金supported by the China Agriculture Research System(Grant No.CARS-23-D06)the Key Research and Development Program of Shaanxi Province(Grant Nos.2024NC2-GJHX-29 and 2024NC-ZDCYL-05-08)Shaanxi Agricultural Collaborative Innovation and Extension Alliance Project(Grant No.LMZD202202).
文摘Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.
基金supported by the Xi'an Key Research and Development Program(Grant No.24NYGG0057)the National Natural Science Foundation of China(Grant No.U22A20491)the Shaanxi Key Research and Development Program(Grant No.2024NC-YBXM-024).
文摘Although both abscisic acid(ABA)and methyl jasmonate(MeJA)play significant roles in regulating the development and quality of grape(Vitis vinifera L.)berries,the regulatory effects and mechanisms of the combined application of ABA and MeJA remain unclear.To further explore the optimal combination of these hormones for regulating the development of grape quality,combined ABA and MeJA treatments were carried out in this study,with‘Jumeigui’grape used as the material.The results indicated that the combined treatment of high-ABA and low-MeJA(HA+LM)increased the sugar-acid ratio,promoted the accumulation of phenolic substances in grape skins,and resulted in anthocyanin content 168.9%higher than that of the control,significantly enhancing coloration.Additionally,the combined treatment of low-ABA and low-MeJA(LA+LM)was more conducive to the accumulation of phenols in grape,especially phenolic acid and resveratrol,as the total phenolic content increased by 38.96%relative to that of the control.Moreover,the expressions of aroma-related genes were upregulated by the combined high-MeJA treatments.The combined treatment of high-ABA and high-MeJA(HA+HM)markedly increased terpene biosynthesis,followed by the LA+HM treatment,increasing the intensity of the rose flavor characteristics of the‘Jumeigui’grape.Therefore,the combination of MeJA and ABA at different concentrations had distinct effects on fruit quality and appropriate combinations can be selected according to the specific needs for the targeted metabolites.
基金supported by the National Natural Science Foundation of China[Grant No.71773091]the Graduate Student Science and Technology Innovation Program of College of Economics and Management[Grant No.JGYJSCXXM202308].
文摘The Grassland Ecological Compensation Policy(GECP)is a large-scale project that has been investing in China since 2011.Despite the significant investment and long duration of the GECP,its impact on carbon sequestration in grasslands remains unclear.Based on panel data from prefecture-level cities in the Yellow River Basin(YRB)from 2000 to 2020,this study explored the effects of the GECP on grassland carbon sequestration in the YRB and its heterogeneity using a time-varying difference-in-differences(TV-DID)model.Subsequently,we predicted changes in carbon sequestration in grasslands under the influence of the GECP from 2021 to 2040 using a simulation.The main conclusions are as follows:First,the implementation of the GECP promoted carbon sequestration in grasslands.This conclusion was validated after conducting a series of robustness tests.Moreover,the effects of the GECP were heterogeneous across different geographical locations,highway densities,and grassland areas.Finally,the simulation results indicated that over the next 20 years,grassland carbon sequestration will generally increase but exhibit cyclical changes.The government should align the principles and objectives of the GECP with local geographic conditions,resource availability,and socio-economic development.It must formulate and implement tailored policies that maximize the ecological protection benefits of the GECP and promote carbon sequestration in the grasslands of the YRB.
基金financially supported by Xinjiang Major Science and Technology Projects,China (2023A02009)。
文摘In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.
基金funded by the Key R&D Program of Shaanxi province(Grant Nos.2023-ZDLNY-23 and 2023XCZX2-18)the National Key R&D Program of China(Grant No.2019YFD1001405).
文摘Proanthocyanidins(PAs)are a group of oligomeric flavonoids also known as condensed tannins.PAs in human diet have antioxidant and health-promoting effects.BSISTER(BS)promotes the accumulation of PAs,but its direct target gene and regulation mechanism in PAs biosynthesis are not clear.Here,we reported VviBS1 and VviBS2 genes,which bind directly to the promoter of PA synthesis key genes VviANR(ANTHOCYANIDIN REDUCTASE)and VviLAR(LEUCOANTHOCYANIDIN REDUCTASE)and up-regulate their expression to promote the PAs biosynthesis in grapevine.VviBS1 and VviBS2 partially rescued the transparent testa phenotype of an Arabidopsis tt16 mutant.Overexpression of BS1 and BS2 in grapevine callus increased the content of PAs,as well as the expression of specific genes related to PA synthesis.VviBS1 and VviBS2 proteins bound directly to the promoter regions of the key PA synthesis genes VviANR1,VviANR2,and VviLAR1.Overexpression of VviBS1 and VviBS2 promotes VviANR1,VviANR2 and VviLAR1 expression and PAs biosynthesis.Some BS1-and BS2-interacting MADS-box proteins have an effect on PAs biosynthesis.This study provides insight into the regulatory mechanisms of PAs biosynthesis in grapevine,which could be effectively employed for metabolic engineering to increase PA content.
基金supported by the National Science Fund for Excellent Young Scholars(grant number 32422082)the Natural Science Basic Research Plan in Shaanxi Province(grant number 2025JC-QYXQ-009)。
文摘Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenesis efficiency through allosteric regulation,redox state,and signal transduction pathways.However,the liver-enriched metabolites that regulate hepatic gluconeogenesis in dairy cows and their specific regulatory mechanisms remain incompletely characterized.Results Six Holstein dairy cows and six Duroc×(Landrace×Yorkshire)(DLY)crossbred pigs served as research subjects.Employing non-targeted and targeted metabolomics,we discovered that three bile acids—taurodeoxycholic acid(TDCA),taurocholic acid(TCA),and glycocholic acid(GCA)—were highly enriched in Holstein dairy cows'livers.In bovine hepatocytes,individual or combined stimulation of these bile acids significantly upregulated the expression of gluconeogenesis genes(FBP1,PCK1 and G6PC)and enhanced glucose production.In fasting mice with induced gluconeogenesis,TDCA,TCA,and GCA increased fasting blood glucose levels,and pyruvate tolerance tests further revealed their capacity to enhance hepatic gluconeogenesis,enabling more efficient glucose synthesis from pyruvate.Mechanistically,these bile acids activated Takeda G protein-coupled receptor 5(TGR5),elevated intracellular cAMP levels,and ultimately enhanced gluconeogenesis via the transcription factor cAMP-response element binding protein(CREB).Notably,a TGR5 inhibitor abrogated the stimulatory effects of TDCA,TCA,and GCA on hepatic gluconeogenesis in fasting mice.Conclusion TDCA,TCA,and GCA are key metabolites promoting hepatic gluconeogenesis in dairy cows,with TGR5 as the pivotal receptor and the cAMP/PKA/CREB pathway as the critical downstream mechanism.
基金funded by the Key R&D Program of Yangling Seed Industry Innovation,China(Ylzy-xm-02)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(2021QNRC001)。
文摘Thinopyrum ponticum(2n=10×=70),a wild relative of common wheat(Triticum aestivum L.),is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.This study focused on the CH97 line,derived from the BC1F7 progeny of a cross between wheat cv.7182 and Th.ponticum.Cytological evidence showed that CH97 has 42 chromosomes,forming 21 bivalents at meiotic metaphase I,with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.Through a combination of fluorescence in situ hybridization(FISH),genomic in situ hybridization(GISH),multicolor GISH(mc-GISH),and liquid array analysis,it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th.ponticum,featuring the absence of a pair of 5D chromosomes and variations in 1B,6B,and 7B chromosomes.These findings confirm that CH97 is a stable wheat-Th.ponticum 5E(5D)alien disomic substitution line.Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period,in contrast to the highly susceptible common wheat parent 7182.Compared to 7182,CH97 displayed improvements in thousand-kernel weight and kernel length.Additionally,utilizing specific-locus amplified fragment sequencing(SLAF-seq)technology,chromosome 5E-specific molecular markers were developed and validated,achieving a 33.3% success rate,facilitating marker-assisted selection for disease resistance in wheat.Overall,the CH97 substitution line,with its resistance to diseases and improved agronomic traits,represents valuable new germplasm for wheat chromosome engineering and breeding.
基金supported by the Special projects on biological seed industry and intensive processing of agricultural products(Grant No.202402AE090004-02)National Key R&D Program of China(Grant No.2023YFD2001404)+1 种基金Technology talent and platform program(Grant No.202305AF150112)China Agriculture Research System of MOF and MARA(Grant No.CARS-29-zp-6).
文摘Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hormone derived from carotenoid,strigolactone(SL)is produced in the roots of plants.It was first reported that SL can induce seed germination of root-parasitic plants.In recent years,it has been shown that strigolactone plays a regulatory role in plant response to abiotic stresses.By eliminating oxidative stress caused by reactive oxygen species,it can potentially increase photosynthetic rate,chlorophyll content,and thus enhance plant drought resistance.Transcriptome studies have explored signal transduction,antioxidant enzyme activity,transcription factors,and expression of stress-and metabolism-related genes induced by extrinsic strigolactone in plants,the effects of strigolactone on plant growth and development have been preliminarily determined,but the studies on inducing crop tolerance to abiotic stresses are still unknown.In this review,the physiological and molecular aspects of the induction of the response to stress in horticultural crops by strigolactone were reviewed.It is important to improve the tolerance and productivity of horticultural crops under abiotic stress.
基金supported by the Key Research and Development Program of Ningxia Hui Autonomous Region,China(2023BCF01029)the Shaanxi Provincial Science and Technology Project for Innovation Team,China(2023-CXTD-59)the Innovative Team Special Project of Northwest A&F University,China(XYTD2023-12)。
文摘Organic management practices and spontaneous fermentations have become focal points in wine research due to increasing consumer interest in healthy foods and sustainable agriculture.In this study,‘Cabernet Sauvignon'grapes sourced from organic and conventional management vineyard(OMV/CMV)in the Ningxia region were subjected to spontaneous fermentation.The microbial,oenological,and aroma profiles of grape must and resulting wines were assessed using high-throughput sequencing(HTS),high-performance liquid chromatography(HPLC),gas chromatography with mass spectrometry(GC-MS),and sensory evaluations.Network analysis was applied to explore relationships among microorganisms,volatile compounds,and aroma attributes.Results showed that organic management significantly increased microbial species richness,α-diversity,and the variety and concentration of aroma compounds,favoring the production of natural wines with complex aroma profiles.Relative abundance of Saccharomyces in OMV reduced,promoting the prevalence of other yeast species during fermentation.Bacterial succession in wines from OMV remained stable,with Pantoea as the dominant genus.Among oenological parameters,OMV wines significantly induced glycerol content,while reduced total acidity,tartaric acid,and citric acid content.These wines exhibited significantly higher levels of fermentative(+16%)and varietal(+72%)volatiles,as well as enhanced floral and sweet fruity aromas,along with distinct nail polish and vegetal notes.Additionally,Saccharomyces,Hanseniaspora,Metschnikowia,and Pantoea were strongly correlated with specific volatile compounds and aroma characteristics.This study provides valuable data that can inform spontaneous fermentation practices and guide vineyard management for natural wine production.
基金funded by the National Key Research&Development Program of China(2023YFD1301400 and 2023YFF1001900)the Program for Shaanxi Science&Technology(2022GD-TSLD-46-0302,2023KXJ-243,2023GXJS-02-01 and L2022-QCYZX-NY-004)。
文摘Background The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention.Taurochenodeoxycholic acid(TCDCA)is the predominant bile acid within the chicken bile acid pool and is closely related to metabolic disorders.The current study aims to investigate the potential effects of TCDCA on abdominal fat deposition in broilers.From 14 to 28 days of age,the broilers in the CON group received an oral administration of 1 mL of saline,while those in the treatment groups were administered 1 mL of a solution containing 0.05 g,0.10 g,or 0.20 g of TCDCA.Results The results showed that TCDCA treatments from 14 to 28 d had no significant effects on BW,ADFI,ADG and FCR in broilers at the age of 28 days of age.However,the abdominal fat percentage in the 0.20 g TCDCA group significantly increased,accompanied by higher TBA and HDL-c levels,as well as a reduction in apolipoprotein B levels in serum.In addition,serum triglyceride levels tended to be higher in the 0.20 g TCDCA group(P=0.098).The 0.20 g TCDCA treatment increased the gene expressions of SREBP-1,C/EBP-α,and ELOVL6,while decreasing the mRNA abundance of ATGL and CPT-1 in the abdominal fat.Serum levels of TCDCA,TDCA,and THDCA were significantly higher after 0.20 g TCDCA administration,while TCA levels were significantly lower,as determined by the targeted bile acid metabolomics analysis.Conversely,hepatic mRNA levels of CYP7A1,CYP27A1,BAAT,and BSEP were increased in the 0.20 g TCDCA group.The oral administration of 0.20 g TCDCA also upregulated the expression of FXR,VDR,and FGF19 in abdominal fat.The 16S rRNA analysis of cecal microbiota revealed that a decrease in the Shannon and Simpson indexes in the 0.20 g TCDCA group,and an increase in the Firmicutes/Bacteroidetes ratio.LEfSe analysis revealed that the predominant bacteria in the CON group were Streptococcus and Oscillospira at the genus level,while Lactobacillus,Parabacteroides,Anaeroplasma,and Helicobacter were identified as the dominant genera in the 0.20 g TCDCA group.Functional predictions for the gut microbiota exhibited that lipid metabolism,replication and repair pathway were enhanced in the 0.20 g TCDCA group.Correlation analysis demonstrated that the abundance of Lactobacillus was positively correlated with serum levels of TCDCA,THDCA,and TDCA,while the abundance of Streptococcus and Oscillospira showed a positive correlation with serum TCA levels.Conclusion Overall,this study elucidates that the intervention of 0.20 g TCDCA may promote abdominal fat deposition by activating bile acid receptors in abdominal fat,and concurrent alterations in both the intestinal microbial community and bile acid profile.
基金supported by the National Public Welfare Forest Desert Shrubbery Monitoring Project。
文摘Desert shrubs are indispensable in maintaining ecological stability by reducing soil erosion,enhancing water retention,and boosting soil fertility,which are critical factors in mitigating desertification processes.Due to the complex topography,variable climate,and challenges in field surveys in desert regions,this paper proposes YOLO-Desert-Shrub(YOLO-DS),a detection method for identifying desert shrubs in UAV remote sensing images based on an enhanced YOLOv8n framework.This method accurately identifying shrub species,locations,and coverage.To address the issue of small individual plants dominating the dataset,the SPDconv convolution module is introduced in the Backbone and Neck layers of the YOLOv8n model,replacing conventional convolutions.This structural optimization mitigates information degradation in fine-grained data while strengthening discriminative feature capture across spatial scales within desert shrub datasets.Furthermore,a structured state-space model is integrated into the main network,and the MambaLayer is designed to dynamically extract and refine shrub-specific features from remote sensing images,effectively filtering out background noise and irrelevant interference to enhance feature representation.Benchmark evaluations reveal the YOLO-DS framework attains 79.56%mAP40weight,demonstrating 2.2%absolute gain versus the baseline YOLOv8n architecture,with statistically significant advantages over contemporary detectors in cross-validation trials.The predicted plant coverage exhibits strong consistency with manually measured coverage,with a coefficient of determination(R^(2))of 0.9148 and a Root Mean Square Error(RMSE)of1.8266%.The proposed UAV-based remote sensing method utilizing the YOLO-DS effectively identify and locate desert shrubs,monitor canopy sizes and distribution,and provide technical support for automated desert shrub monitoring.
基金supported by the National Natural Science Foundation of China(Grant No.32171863).
文摘Anthocyanins are biosynthesized in the endoplasmic reticulum and stored in vacuoles,and glutathione S-transferase(GST)plays a key role in anthocyanin accumulation.However,little is known about the mechanism of the role of GST in this process.We identified a prominent candidate gene,MaGST1,which is highly expressed during the full-bloom stage,in grape hyacinth.MaGST1 rescued the phenotype of the anthocyanin biosynthesis-deficient mutant tt19,indicating that MaGST1 functions in anthocyanin transport.MaGST1-RNAi transgenic petals were generated by stable genetic transformation.We found that MaGST1-RNAi inhibited the accumulation of anthocyanin in petals.Interestingly,in vitro experiments revealed that recombinant MaGST1 increased the water solubilities of cyanidin(Cya),delphinidin 3-O-glucoside(Dp3G),and petunidin 3-O-glucoside(Pt3G).Compared with Dp3G and Pt3G,Cya significantly suppressed the quenching of the intrinsic tryptophan fluorescence of MaGST1 to a lower level,indicating that MaGST1 has a greater affinity for Cya than for Dp3G and Pt3G.Site-directed mutagenesis and anthocyanin water solubility assays revealed that MaGST1 primarily binds to anthocyanin via Ser-68.Furthermore,yeast one-hybrid,dual-luciferase,and GUS staining assays revealed that MaGST1 expression is positively regulated by MaMybA.In summary,our findings reveal the molecular mechanism of anthocyanin transport mediated by MaGST1.
基金supported by the National Natural Science Foundation of China(U2243235)the Shaanxi Provincial Department of Water Resources,China(2022slkj-6)。
文摘The irrigation districts of northern China face issues such as water scarcity,inability to effectively utilize flood resources,and groundwater overexploitation.In view of these challenges,this study proposes a new concept of deep storage irrigation through flood resources utilization.However,whether deep storage irrigation can recharge deep soil moisture and sustain crop production still requires further study.A two-year field experiment was conducted on summer maize in the Guanzhong Plain with five soil wetting layer depths(T1:60 cm;T2:90 cm;T3:120 cm;T4:150 cm;T5:180 cm)and soil saturation moisture content as the irrigation upper limit.The results presented that the ranges of deep soil moisture recharge in the100–200 cm soil profile(SMS_(100–200))was 73.34–267.42 and 0–150.03 mm in 2021(wet season)and 2022(normal season).When the effective precipitation and irrigation exceeded 390 mm,the SMS_(100–200)began to linearly increase.The highest grain yield(GY)were observed at T2 and T3 treatments in 2021(11.44 t ha^(-1))and 2022(11.25 t ha^(-1)),respectively.The maize GY of T4 in 2021 and T5 in 2022 were only 3.9 and 5.7%lower than the maximize GY,respectively.However,the SMS_(100–200)for T4 and T5 were 2.4 and 5.0 times that of T2 and T3 treatments in 2021 and 2022,respectively.Overall,the further increase in irrigation amounts induced only a slight decrease in grain yield,but it significantly increased deep soil moisture recharge.Therefore,the deep storage irrigation breaks through the traditional idea of water-saving irrigation with limited water resources,which can be utilized as an effective alternative to address the issues of water scarcity,low flood resources utilization,and groundwater level declines in the irrigation districts of northern China.
