Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic m...Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic maps is an efficient approach to identify genetic regions for FL.In our study, two backcrossed inbred lines(BILs) were chosen as parents to construct a high-density genetic map in F2 which was used to fine map FL QTL in F2:3 population.The genetic map had a total size of 3462.8 cM, containing 9182 singlenucleotide polymorphisms(SNPs) based on genotyping-by-sequencing.Two FL related stable QTL were identified on two chromosomes(qFL-A08–1 on A08 and qFL-D03–1 on D03),and qFL-A08–1 was confirmed by a meta-analysis.Utilizing previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5(CB5, Gh_A08 G1729) and microtubule end-binding 1 C(EB1C, Gh_D03 G0232) that may regulate FL during the fiber elongation stage were identified.In addition, nine recombination hotspots in this population were found.The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation.展开更多
The Permian Fengcheng Formation of the Western Junggar region in the Southwestern Central Asian Orogenic Belt(CAOB)represents one of Earth’s oldest alkali lake deposits.Here,we present a comprehensive study of the st...The Permian Fengcheng Formation of the Western Junggar region in the Southwestern Central Asian Orogenic Belt(CAOB)represents one of Earth’s oldest alkali lake deposits.Here,we present a comprehensive study of the stratigraphy,petrography,two-dimensional seismic data,U–Pb geochronology,and Hf isotope analysis of detrital zircons of this deposit.The results,in conjunction with published data,reflect the tectonic evolution of southwestern CAOB.The ages of detrital zircons indicate that the Fengcheng Formation deposition is inferred to have concluded the early Permian Kungurian.The Hf isotopes of detrital zircons indicate that the detritus for the Fengcheng Formation was derived from upper crustal magmatic sources.The West Junggar Basin preserves the records of three Paleozoic tectonic stages.The first stage occurred in the Early Paleozoic and involved intraoceanic subduction and arc-continent collision.The second stage involved the Carboniferous closure of the Junggar Ocean following successive filling of oceanic basins.The final stage occurred in the Early Permian and was related to intracontinental rifting and tectonic inversion.The results of comparing the comprehensive data of U–Pb ages and Hf isotopes of 2537 zircons from West Junggar,Tianshan and Altay show that the orogenic belts to the south of the CAOB experienced similar plate kinematics and vertical crustal growth in the Paleozoic.展开更多
Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-...Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-response gene GhKLCR1, which is a close homolog of AtKLCR1, which encodes a kinesin light chain-related protein enriched with a tetratrico peptide-repeat region.A subcellular localization assay showed that GhKLCR1 is associated with the cell membrane. A tissue-specific expression profile analysis demonstrated that GhKLCR1 is a cotton root-specific gene. Further abiotic and hormonal stress treatments showed that GhKLCR1 was upregulated during abiotic stresses, especially after polyethylene glycol treatments. In addition, the glucuronidase(GUS) staining activity increased as the increment of mannitol concentration in transgenic Arabidopsis plants harboring the fusion construct PGhKLCR1::GUS. The root lengths of 35 S::GhKLCR1 lines were significantly reduced compared with that of wild type. Additionally, seed germination was strongly inhibited in 35 S::GhKLCR1 lines after 300-mmol L^(-1) mannitol treatments as compared with Columbia-0, indicating the sensitivity of GhKLCR1 to drought. These findings provide a better understanding of the structural, physiological and functional mechanisms of kinesin light chain-related proteins.展开更多
基金supported by the National Natural Science Foundation of China (31621005)the National Key Research and Development Program of China (2016YFD0101400)+1 种基金the National Transgenic Research Program of China (2016ZX08005005)the New Mexico Agricultural Experiment Station
文摘Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic maps is an efficient approach to identify genetic regions for FL.In our study, two backcrossed inbred lines(BILs) were chosen as parents to construct a high-density genetic map in F2 which was used to fine map FL QTL in F2:3 population.The genetic map had a total size of 3462.8 cM, containing 9182 singlenucleotide polymorphisms(SNPs) based on genotyping-by-sequencing.Two FL related stable QTL were identified on two chromosomes(qFL-A08–1 on A08 and qFL-D03–1 on D03),and qFL-A08–1 was confirmed by a meta-analysis.Utilizing previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5(CB5, Gh_A08 G1729) and microtubule end-binding 1 C(EB1C, Gh_D03 G0232) that may regulate FL during the fiber elongation stage were identified.In addition, nine recombination hotspots in this population were found.The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation.
基金supported by the funding of the National Natural Science Foundation of China(Nos.41802177,42272188)Petrochina Technology Project(Nos.2021DJ0206,2022DJ0507,2020D-5008-04).
文摘The Permian Fengcheng Formation of the Western Junggar region in the Southwestern Central Asian Orogenic Belt(CAOB)represents one of Earth’s oldest alkali lake deposits.Here,we present a comprehensive study of the stratigraphy,petrography,two-dimensional seismic data,U–Pb geochronology,and Hf isotope analysis of detrital zircons of this deposit.The results,in conjunction with published data,reflect the tectonic evolution of southwestern CAOB.The ages of detrital zircons indicate that the Fengcheng Formation deposition is inferred to have concluded the early Permian Kungurian.The Hf isotopes of detrital zircons indicate that the detritus for the Fengcheng Formation was derived from upper crustal magmatic sources.The West Junggar Basin preserves the records of three Paleozoic tectonic stages.The first stage occurred in the Early Paleozoic and involved intraoceanic subduction and arc-continent collision.The second stage involved the Carboniferous closure of the Junggar Ocean following successive filling of oceanic basins.The final stage occurred in the Early Permian and was related to intracontinental rifting and tectonic inversion.The results of comparing the comprehensive data of U–Pb ages and Hf isotopes of 2537 zircons from West Junggar,Tianshan and Altay show that the orogenic belts to the south of the CAOB experienced similar plate kinematics and vertical crustal growth in the Paleozoic.
基金supported by the National Natural Science Foundation of China (31501345)
文摘Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-response gene GhKLCR1, which is a close homolog of AtKLCR1, which encodes a kinesin light chain-related protein enriched with a tetratrico peptide-repeat region.A subcellular localization assay showed that GhKLCR1 is associated with the cell membrane. A tissue-specific expression profile analysis demonstrated that GhKLCR1 is a cotton root-specific gene. Further abiotic and hormonal stress treatments showed that GhKLCR1 was upregulated during abiotic stresses, especially after polyethylene glycol treatments. In addition, the glucuronidase(GUS) staining activity increased as the increment of mannitol concentration in transgenic Arabidopsis plants harboring the fusion construct PGhKLCR1::GUS. The root lengths of 35 S::GhKLCR1 lines were significantly reduced compared with that of wild type. Additionally, seed germination was strongly inhibited in 35 S::GhKLCR1 lines after 300-mmol L^(-1) mannitol treatments as compared with Columbia-0, indicating the sensitivity of GhKLCR1 to drought. These findings provide a better understanding of the structural, physiological and functional mechanisms of kinesin light chain-related proteins.
