The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a mol...The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.展开更多
随着汽车的电子电气架构向域集中和区域集中方向发展,电子控制单元(Electronic Control Unit,ECU)需要更高的计算能力和更多样的接口,导致核心芯片的工作电压逐渐降低、工作速度加快、功耗增加,对车规微控制器单元(Microcontroller Unit...随着汽车的电子电气架构向域集中和区域集中方向发展,电子控制单元(Electronic Control Unit,ECU)需要更高的计算能力和更多样的接口,导致核心芯片的工作电压逐渐降低、工作速度加快、功耗增加,对车规微控制器单元(Microcontroller Unit,MCU)的性能提出了更高要求。文章提出一种基于目标阻抗的去耦设计方法,结合电容等效模型和电容去耦原理分析了0~50 MHz频率范围内电源分配网络(Power Distribution Network,PDN)的阻抗,通过Cadence Optimize PI电源完整性仿真软件对MCU核心电源进行仿真。结果表明,该方法设计的PDN能够满足芯片的电源需求,避免了过度设计,从而提高了开发效率。展开更多
为加速有限元分析流程,提出了一种面向有限元分析的自适应三角网格模型布尔运算方法。将ADT(alternating digital tree)数据结构应用于三角面片的判交计算,提升了布尔运算中三角面片的判交效率;借鉴结合球填充算法和插点/去点算法的网...为加速有限元分析流程,提出了一种面向有限元分析的自适应三角网格模型布尔运算方法。将ADT(alternating digital tree)数据结构应用于三角面片的判交计算,提升了布尔运算中三角面片的判交效率;借鉴结合球填充算法和插点/去点算法的网格重生成方法来重剖分局部区域,保证了算法的效率和生成网格的质量;通过改进的八叉树背景网格来记录和光滑尺寸场,生成了自适应的网格;通过尺寸场合理地控制重剖分区域,使算法效率和生成网格的质量得以很好地平衡。实验结果表明,所提方法可以高效地生成满足有限元分析需求的网格。展开更多
基金supported by grants from the National Key Research and Development Program of China(Nos.2023YFA0915200,2023YFA0915204)the Equipment Research and Development Projects of the Chinese Academy of Sciences(No.PTYQ2024YZ0010)+3 种基金the Science and Technology Commission of Shanghai Municipality Project(No.XTCX-KJ-2024-038)the Natural Science Foundation of Hebei Province of China(No.H2024206249)the Postdoctoral Fellowship Program of CPSF(No.GZC20232838)Science and Technology Commission of Shanghai Municipality(No.22S31901700).
文摘The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.
文摘随着汽车的电子电气架构向域集中和区域集中方向发展,电子控制单元(Electronic Control Unit,ECU)需要更高的计算能力和更多样的接口,导致核心芯片的工作电压逐渐降低、工作速度加快、功耗增加,对车规微控制器单元(Microcontroller Unit,MCU)的性能提出了更高要求。文章提出一种基于目标阻抗的去耦设计方法,结合电容等效模型和电容去耦原理分析了0~50 MHz频率范围内电源分配网络(Power Distribution Network,PDN)的阻抗,通过Cadence Optimize PI电源完整性仿真软件对MCU核心电源进行仿真。结果表明,该方法设计的PDN能够满足芯片的电源需求,避免了过度设计,从而提高了开发效率。
文摘为加速有限元分析流程,提出了一种面向有限元分析的自适应三角网格模型布尔运算方法。将ADT(alternating digital tree)数据结构应用于三角面片的判交计算,提升了布尔运算中三角面片的判交效率;借鉴结合球填充算法和插点/去点算法的网格重生成方法来重剖分局部区域,保证了算法的效率和生成网格的质量;通过改进的八叉树背景网格来记录和光滑尺寸场,生成了自适应的网格;通过尺寸场合理地控制重剖分区域,使算法效率和生成网格的质量得以很好地平衡。实验结果表明,所提方法可以高效地生成满足有限元分析需求的网格。
