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家兔供体卵裂球细胞周期对核移植胚胎发育的影响 被引量:2
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作者 王永胜 曾申明 +7 位作者 朱士恩 田见晖 余文莉 李树静 张忠诚 陈永福 商家骥 魏建生 《动物学报》 SCIE CAS CSCD 北大核心 2002年第3期368-374,共7页
通过化学药物处理使家兔桑椹胚卵裂球的细胞周期分别同期化于G1、S或G2期 ,然后分别移入去核的MⅡ期卵母细胞中 ,以研究供体核细胞周期对家兔胚胎细胞核移植效率的影响。试验结果表明供体核细胞周期对家兔核移植胚胎的发育潜力有明显影... 通过化学药物处理使家兔桑椹胚卵裂球的细胞周期分别同期化于G1、S或G2期 ,然后分别移入去核的MⅡ期卵母细胞中 ,以研究供体核细胞周期对家兔胚胎细胞核移植效率的影响。试验结果表明供体核细胞周期对家兔核移植胚胎的发育潜力有明显影响 ,以G1期卵裂球为供体的核移植重组胚的激活率、卵裂率、桑椹胚、囊胚和孵化囊胚率及囊胚平均细胞数分别为 87 3% (32 2 / 36 9)、 84 9% (2 99/ 35 2 )、 71 5 % (193/ 2 70 )、5 8 0 % (76 / 131)、 35 1% (4 6 / 131)和 12 6± 4 8,显著高于S期 [79 6 % (10 9/ 137)、 74 4% (93/ 12 5 )、30 3% (33/ 10 9)、19 3% (17/ 88)、 6 8% (6 / 88)和 118 8± 3 5 ]、G2期 [6 3 6 % (70 / 110 )、6 0 % (6 0 / 10 0 )、16 9% (11/ 6 5 )、 16 3% (7/ 43)、 4 7% (2 / 43)和 110 6± 5 8]和未经同期化处理的卵裂球 [78 1% (185 /2 37)、 73 2 % (15 8/ 2 16 )、 49 4% (4 3/ 87)、 32 2 % (2 8/ 87)、 12 6 % (11/ 87)和 12 0 5± 4 4](P <0 0 5 )。来源于G1期卵裂球的 144枚克隆胚胎移植到 12只受体中 ,6只妊娠并产下 19只活仔 ,产仔率为 13 2 % ,显著高于来源于S期 (5 8% ,7/ 12 0 )、G2期 (0 ,0 / 12 6 )或未同期化卵裂球的克隆胚胎 (5 3% ,8/ 15 0 ) (P 展开更多
关键词 家兔 供体 卵裂球 细胞周期 核移植 胚胎发育
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Real-Time Detection of Cracks on Concrete Bridge Decks Using Deep Learning in the Frequency Domain 被引量:10
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作者 Qianyun Zhang Kaveh Barri +1 位作者 Saeed K.Babanajad Amir H.Alavi 《Engineering》 SCIE EI 2021年第12期1786-1796,共11页
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequen... This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM algorithm is trained using thousands of images of cracked and non-cracked concrete bridge decks.In order to improve the training efficiency,images are first transformed into the frequency domain during a preprocessing phase.The algorithm is then calibrated using the flattened frequency data.LSTM is used to improve the performance of the developed network for long sequence data.The accuracy of the developed model is 99.05%,98.9%,and 99.25%,respectively,for training,validation,and testing data.An implementation framework is further developed for future application of the trained model for large-scale images.The proposed 1D-CNN-LSTM method exhibits superior performance in comparison with existing deep learning methods in terms of accuracy and computation time.The fast implementation of the 1D-CNN-LSTM algorithm makes it a promising tool for real-time crack detection. 展开更多
关键词 Crack detection Concrete bridge deck Deep learning REAL-TIME
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Assessment of buckling-restrained braced frame reliability using an experimental limit-state model and stochastic dynamic analysis 被引量:1
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作者 Blake M.Andrews Junho Song Larry A.Fahnestock 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2009年第3期373-385,共13页
Buckling-restrained braces (BRBs) have recently become popular in the United States for use as primary members of seismic lateral-force-resisting systems. A BRB is a steel brace that does not buckle in compression b... Buckling-restrained braces (BRBs) have recently become popular in the United States for use as primary members of seismic lateral-force-resisting systems. A BRB is a steel brace that does not buckle in compression but instead yields in both tension and compression. Although design guidelines for BRB applications have been developed, systematic procedures for assessing performance and quantifying reliability are still needed. This paper presents an analytical framework for assessing buckling-restrained braced frame (BRBF) reliability when subjected to seismic loads. This framework efficiently quantifies the risk of BRB failure due to low-cycle fatigue fracture of the BRB core. The procedure includes a series of components that: (1) quantify BRB demand in terms of BRB core deformation histories generated through stochastic dynamic analyses; (2) quantify the limit-state of a BRB in terms of its remaining cumulative plastic ductility capacity based on an experimental database; and (3) evaluate the probability of BRB failure, given the quantified demand and capacity, through structural reliability analyses. Parametric studies were conducted to investigate the effects of the seismic load, and characteristics of the BRB and BRBF on the probability of brace failure. In addition, fragility curves (i.e., conditional probabilities of brace failure given ground shaking intensity parameters) were created by the proposed framework. While the framework presented in this paper is applied to the assessment of BRBFs, the modular nature of the framework components allows for application to other structural components and systems. 展开更多
关键词 risk and reliability analysis buckling-restrained brace stochastic dynamic analysis first-order reliability method cumulative plastic ductility capacity
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A phased approach to enable hybrid simulation of complex structures
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作者 Billie F.Spencer Jr. +4 位作者 Chia-Ming Chang Thomas M.Frankie Daniel A.Kuchma Pedro F.Silva Adel E.Abdelnaby 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2014年第S1期63-77,共15页
Hybrid simulation has been shown to be a cost-effective approach for assessing the seismic performance of structures. In hybrid simulation,critical parts of a structure are physically tested,while the remaining portio... Hybrid simulation has been shown to be a cost-effective approach for assessing the seismic performance of structures. In hybrid simulation,critical parts of a structure are physically tested,while the remaining portions of the system are concurrently simulated computationally,typically using a finite element model. This combination is realized through a numerical time-integration scheme,which allows for investigation of full system-level responses of a structure in a cost-effective manner. However,conducting hybrid simulation of complex structures within large-scale testing facilities presents significant challenges. For example,the chosen modeling scheme may create numerical inaccuracies or even result in unstable simulations; the displacement and force capacity of the experimental system can be exceeded; and a hybrid test may be terminated due to poor communication between modules(e.g.,loading controllers,data acquisition systems,simulation coordinator). These problems can cause the simulation to stop suddenly,and in some cases can even result in damage to the experimental specimens; the end result can be failure of the entire experiment. This study proposes a phased approach to hybrid simulation that can validate all of the hybrid simulation components and ensure the integrity largescale hybrid simulation. In this approach,a series of hybrid simulations employing numerical components and small-scale experimental components are examined to establish this preparedness for the large-scale experiment. This validation program is incorporated into an existing,mature hybrid simulation framework,which is currently utilized in the Multi-Axial Full-Scale Sub-Structuring Testing and Simulation(MUST-SIM) facility of the George E. Brown Network for Earthquake Engineering Simulation(NEES) equipment site at the University of Illinois at Urbana-Champaign. A hybrid simulation of a four-span curved bridge is presented as an example,in which three piers are experimentally controlled in a total of 18 degrees of freedom(DOFs). This simulation illustrates the effectiveness of the phased approach presented in this paper. 展开更多
关键词 hybrid simulation seismic performance evaluation phased approach curved bridge MUST-SIM facility
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COMPARISON OF GREEN HOME ENERGY PERFORMANCE BETWEEN SIMULATION AND OBSERVATION: A CASE OF VIRGINIA, UNITED STATES 被引量:2
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作者 Andrew P.McCoy Dong Zhao +2 位作者 Teni Ladipo Philip Agee Yunjeong Mo 《Journal of Green Building》 2018年第3期70-88,共19页
The United States has a long-term goal to reduce 50%of energy usage in buildings based on 2010 consumption levels.Home energy efficiency is often measured by laboratory experiments and computational simulation.Thus,th... The United States has a long-term goal to reduce 50%of energy usage in buildings based on 2010 consumption levels.Home energy efficiency is often measured by laboratory experiments and computational simulation.Thus,there is little to no quantifiable evidence showing the extent of energy efficiency homes can achieve within the larger context of green building standards.The objective of this research is to identify actual home energy performance as an effect of green building technolo-gies by comparing energy use from real-world observations and energy modeling.Results indicate a significant reduction of energy consumption at 43.7%per unit or 43.4%per square foot(i.e.,0.093 m^(2))and substantial financial savings at$628.4 per unit or$0.80 per square foot(i.e.,$8.6 per m^(2))annually.Savings account for 2%of median annual household income or 46%of energy cost expenditures for an American home.Results also identify the construction type as a significant factor,yet building technology is not the only factor influencing a home’s energy efficiency.The findings contribute to the body of knowledge in three aspects:(1)simulated energy usage is higher than actual energy usage;(2)energy modeling via simulation tools is particularly accurate for new construction;and(3)energy modeling,especially for existing buildings,is not accurate due to largely varying occupant behaviors. 展开更多
关键词 building construction SUSTAINABILITY HOUSING energy efficiency environmental systems energy simulation
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