Increasing urbanization rates observed in the last decades have significantly contributed to a higher number of travelers to urban environments. The objective of this study was to identify measures and factors that en...Increasing urbanization rates observed in the last decades have significantly contributed to a higher number of travelers to urban environments. The objective of this study was to identify measures and factors that encourage the use of alternative transportation in the cities of Washington D.C., USA and Porto Alegre, Brazil. The methodology used to compare these two cities was an evaluation of their urban mobility plans, including strategic analyses and measures to promote the use of public transport. This approach was based on factors described in the following document： ＂Urban Sustainability： Impacts of Economic Development and Their Consequences on the Process of Urbanization in Emerging Countries＂, prepared by the United Nations. The results indicate different planning strategies, ranging from focusing on tax integration policies in order to become more financially attractive to the user, to using different modes of mass transportation integrated with active mobility. In Washington D.C., an innovative strategy was developed in preparation to forecast scenarios, as the measures comprised projection for the year 2040, which will respond to mobility demands in the coming decades.展开更多
Yakima County, Washington, a rural county with an urban core suffered disproportionately under the conditions presented by the COVID-19 pandemic and summer wildfires of 2020. With an infection rate of over 700 per 100...Yakima County, Washington, a rural county with an urban core suffered disproportionately under the conditions presented by the COVID-19 pandemic and summer wildfires of 2020. With an infection rate of over 700 per 100,000 population at the height of the pandemic, the county concurrently experienced 14 consecutive days of an air quality index in the unhealthy to hazardous range in August 2020. This paper examines the contributing socioeconomic, geographic, and environmental vulnerabilities that make Yakima County particularly susceptible to the continuum of expected COVID-19 disease and related outcomes and suggests comprehensive areas of investigation to mitigate its impact on special populations, including Hispanic-Latino communities, agricultural, food production, and other essential workers.展开更多
Vegetation and soil within estuarine ecosystems play an integral role in ecological processes within pocket estuaries. However, physical barriers, caused by culverts diminish hydrological inputs, sediment exchange, an...Vegetation and soil within estuarine ecosystems play an integral role in ecological processes within pocket estuaries. However, physical barriers, caused by culverts diminish hydrological inputs, sediment exchange, and habitat connectivity. The restoration of estuaries by bridge replacement reconnects <span>the aquatic corridor, however, the recovery of plant communities and soil s</span>ubstrate is not well understood. This observational study monitored four estuary restoration sites of variable ages (0 - 12 years) in Western Washington, USA. Plant community composition, soil organic carbon, organic matter, and soil nutrients were assessed. Percent soil carbon was different among the pre-restoration and youngest (3-year) post-restoration site (<i>P</i> = 0.03), suggesting an initial decrease in carbon and organic matter during restoration. Both N and P were deficient at the newest, lower restoration site, presumably linked to the lack of organic matter required for adequate cation exchange capacity and nutrient/plant exchange (<i>P</i> < 0.05). Plant species diversity was higher at the intermediate (9-year) and oldest post-restoration sites (12-year;<i>P</i> = 0.02). Vegetation composition was primarily native species with few invasive plants present. The results of this study illustrate that tidal marsh plant communities are influenced by the development of salinity and vertical gradients with older sites having an increase in species diversity. Future surveys are ongoing to <span>better understand how these sites recover organic matter and tidal marsh co</span>mmunities to form adequate estuarine habitat over time.展开更多
The University of Washington has played a pivotal role in the field of sinology with faculty and alumni producing major publications in Chinese history,literature,phonetics,and linguistics.These contributions have bee...The University of Washington has played a pivotal role in the field of sinology with faculty and alumni producing major publications in Chinese history,literature,phonetics,and linguistics.These contributions have been instrumental in the development of sinology as a field and have both directly and indirectly influenced the study of Chinese Medicine.By tracing the history of the Department of Asian Languages and Literature and examining several major figures,we can better understand how these individuals shaped the development of Chinese Medicine and contributed to its spread worldwide.展开更多
In the United States, type 2 diabetes mellitus (T2DM) disproportionately affects the African American (AA) community, which has not been systematically included in molecular studies of underlying mechanisms. As part o...In the United States, type 2 diabetes mellitus (T2DM) disproportionately affects the African American (AA) community, which has not been systematically included in molecular studies of underlying mechanisms. As part of a gene expression study, we recruited cases with T2DM and matched, unaffected controls at an urban hospital in Washington, DC, with a majority AA population. Here we describe the profile of socio-demographic, behavioral, and health-related associations of the study population. Self-reported data were collected from cases with T2DM (N = 77) and age- and gender-matched controls (N = 80), ages 45 - 65 years. Logistic regression was used to calculate odds ratios (OR) and 95% confidence intervals (CI). As expected, obesity, hypertension, and cardiovascular disease were more prevalent in cases than in controls. Tobacco smoking and working alongside other tobacco smokers were also associated with T2DM. After adjusting for covariates, current tobacco smoking remained statistically associated with the disease (OR per half pack of cigarettes 1.43, 95% CI 1.04 - 1.95;p-value 0.027). HbA1c levels were elevated in T2DM cases who smoked more than a pack of cigarettes daily. These associations highlight the comorbid burdens of T2DM in an AA urban community setting and identify tobacco control as an unmet need for future prevention and control efforts.展开更多
Due to federal, state, and local regulations pertaining to clean water and salmon recovery, stream health and water quality are major concerns in the Pacific Northwest of the United States. An integral component of mo...Due to federal, state, and local regulations pertaining to clean water and salmon recovery, stream health and water quality are major concerns in the Pacific Northwest of the United States. An integral component of most riparian rehabilitation efforts is the effective establishment of preferred vegetation. We conducted arthropod surveys in representative disturbed and rehabilitated riparian buffers directly adjacent to apple orchards and grape vineyards in the Lower Yakima Valley near Prosser, Benton County, Washington State USA. Objectives of the study were to determine whether densities of select predatory and pest taxa changed with distance from the waterway and to test whether densities differed among disturbed, rehabilitated, or pristine riparian buffers. The studies suggest that populations of some beneficial arthropods, including spiders and ground beetles, were higher in the presence of exotic flowering plant species. In the riparian environments surveyed, more native woody plant species were found adjacent to the waterway, and this apparent stability appears to have had a positive effect on beneficial arthropods. Concurrently, data indicated that some exotic, flowering species of plants may be preferred over native plant species as hosts to certain arthropod pests. These plants tend to persist in degraded riparian buffers and at the interface of riparian habitats and cultivated areas, whereas native plant species are predominant in pristine or properly maintained and rehabilitated buffers.展开更多
The East Asia Library(EAZ), following the leadership of the University of Washington(UW) Libraries and the trends of the library world, has made great progress in library automation. Yet the presence of non-roman scri...The East Asia Library(EAZ), following the leadership of the University of Washington(UW) Libraries and the trends of the library world, has made great progress in library automation. Yet the presence of non-roman scripts, or CJK characters, in the library's electronic resources in an environment dominated by phonetic languages makes automation of East Asian Libraries more challenging than libraries which use only roman scripts. This paper first provides a general overview of current information technology. It then offers a brief introduction to the UW Library automation and how the East Asia Library handles automation Finally, it presents the experience of the information technology in Chinese studies at EAL.展开更多
Spatiotemporal analysis of fire activity is vital for determining why wildfires occur where they do,assessing wildfire risks,and developing locally relevant wildfire risk reduction strategies.Using various spatial sta...Spatiotemporal analysis of fire activity is vital for determining why wildfires occur where they do,assessing wildfire risks,and developing locally relevant wildfire risk reduction strategies.Using various spatial statistical methods,we determined hot spots of large wildfires(>100 acres)in Washington,the United States,and mapped spatiotemporal variations in large wildfire activity from 1970 to 2020.Our results found that all hot spots are located east of the crest of the Cascade Range.Our spatiotemporal analysis found that the geographic area wherein most of the state’s acres burned has shrunk considerably since 1970 and has become concentrated over the north-central portion of the state over time.This concentration of large wildfire activity in north-central Washington was previously unquantified and may provide important information for hazard mitigation efforts in that area.Our results highlight the advantages of using spatial statistical methods that could aid the development of natural hazard mitigation plans and risk reduction strategies by characterizing previous hazard occurrences spatially and spatiotemporally.展开更多
Regenerative capacity of the central nervous system(CNS)is unevenly distributed among vertebrates.While most mammalian species including humans elicit limited repair following CNS injury or disease,highly regenerative...Regenerative capacity of the central nervous system(CNS)is unevenly distributed among vertebrates.While most mammalian species including humans elicit limited repair following CNS injury or disease,highly regenerative vertebrates including urodele amphibians and teleost fish spontaneously reverse CNS damage.Teletost zebrafish(danio rerio)are tropical freshwater fish that proved to be an excellent vertebrate model of successful CNS regeneration.Differential neuronal,glial,and immune injury responses underlie disparate injury outcomes between highly regenerative zebrafish and poorly regenerative mammals.This article describes complications associated with neuronal repair following spinal cord injury(SCI)in poorly regenerative mammals and highlights intersecting modes of plasticity and regeneration in highly regenerative zebrafish(Figures 1 and 2).Comparative approaches evaluating immunoglial SCI responses were recently reviewed elsewhere(Reyes and Mokalled,2024).展开更多
Calcium (Ca^(2+)) is a key intracellular messenger involved in a variety of cellular functions.Intracellular Ca^(2+)dysregulation drives neuron cell death in multiple degenerative diseases and traumatic conditions.Ret...Calcium (Ca^(2+)) is a key intracellular messenger involved in a variety of cellular functions.Intracellular Ca^(2+)dysregulation drives neuron cell death in multiple degenerative diseases and traumatic conditions.Retinal ganglion cell(RGC) degeneration occurs in blinding diseases such as glaucoma and other optic neuropathies.展开更多
Chemical warfare agents(CWAs)remain a persistent hazard in many parts of the world,necessitating a deeper exploration of their chemical and physical characteristics and reactions under diverse conditions.Diisopropyl m...Chemical warfare agents(CWAs)remain a persistent hazard in many parts of the world,necessitating a deeper exploration of their chemical and physical characteristics and reactions under diverse conditions.Diisopropyl methylphosphonate(DIMP),a commonly used CWA surrogate,is widely studied to enhance our understanding of CWA behavior.The prevailing thermal decomposition model for DIMP,developed approximately 25 years ago,is based on data collected in nitrogen atmospheres at temperatures ranging from 700 K to 800 K.Despite its limitations,this model continues to serve as a foundation for research across various thermal and reactive environments,including combustion studies.Our recent experiments have extended the scope of decomposition analysis by examining DIMP in both nitrogen and zero air across a lower temperature range of 175℃ to 250℃.Infrared spectroscopy results under nitrogen align well with the established model;however,we observed that catalytic effects,stemming from decomposition byproducts and interactions with stainless steel surfaces,alter the reaction kinetics.In zero air environments,we observed a novel infrared absorption band.Spectral fitting suggests this band may represent a combination of propanal and acetone,while GCMS analysis points to vinyl formate and acetone as possible constituents.Although the precise identity of these new products remains unresolved,our findings clearly indicate that the existing decomposition model cannot be reliably extended to lower temperatures or non-nitrogen environments without further revisions.展开更多
Retinal ganglion cells are susceptible to neurodegenerative conditions and their death drives common forms of irreversible vision loss.In mice,there are 46 transcriptionally unique retinal ganglion cell types that dem...Retinal ganglion cells are susceptible to neurodegenerative conditions and their death drives common forms of irreversible vision loss.In mice,there are 46 transcriptionally unique retinal ganglion cell types that demonstrate different susceptibilities to degeneration.Recent transcriptional experiments defined a novel retinal ganglion cell type that survives particularly well and uniquely expresses high levels of the orphan G-protein-coupled receptor 88.Motivated to study this retinal ganglion cell type,we obtained GPR88-Cre transgenic mice to identify the novel well-surviving retinal ganglion cells and examine their survival and regenerative potential.Our experiments demonstrate that this unidentified retinal ganglion cell type is likely accordant with previously described ON-direction-selective retinal ganglion cells.Interestingly,we find that ON-direction-selective retinal ganglion cells are resilient,but demonstrate limited potential to regenerate their axons in response to well-characterized regenerative treatments.Studying the molecular properties of the ON-direction-selective retinal ganglion cells could unlock new therapeutics to preserve retinal ganglion cells in patients.展开更多
Early and accurate detection of bone cancer and marrow cell abnormalities is critical for timely intervention and improved patient outcomes.This paper proposes a novel hybrid deep learning framework that integrates a ...Early and accurate detection of bone cancer and marrow cell abnormalities is critical for timely intervention and improved patient outcomes.This paper proposes a novel hybrid deep learning framework that integrates a Convolutional Neural Network(CNN)with a Bidirectional Long Short-Term Memory(BiLSTM)architecture,optimized using the Firefly Optimization algorithm(FO).The proposed CNN-BiLSTM-FO model is tailored for structured biomedical data,capturing both local patterns and sequential dependencies in diagnostic features,while the Firefly Algorithm fine-tunes key hyperparameters to maximize predictive performance.The approach is evaluated on two benchmark biomedical datasets:one comprising diagnostic data for bone cancer detection and another for identifying marrow cell abnormalities.Experimental results demonstrate that the proposed method outperforms standard deep learning models,including CNN,LSTM,BiLSTM,and CNN-LSTM hybrids,significantly.The CNNBiLSTM-FO model achieves an accuracy of 98.55%for bone cancer detection and 96.04%for marrow abnormality classification.The paper also presents a detailed complexity analysis of the proposed algorithm and compares its performance across multiple evaluation metrics such as precision,recall,F1-score,and AUC.The results confirm the effectiveness of the firefly-based optimization strategy in improving classification accuracy and model robustness.This work introduces a scalable and accurate diagnostic solution that holds strong potential for integration into intelligent clinical decision-support systems.展开更多
Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewa...Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.展开更多
文摘Increasing urbanization rates observed in the last decades have significantly contributed to a higher number of travelers to urban environments. The objective of this study was to identify measures and factors that encourage the use of alternative transportation in the cities of Washington D.C., USA and Porto Alegre, Brazil. The methodology used to compare these two cities was an evaluation of their urban mobility plans, including strategic analyses and measures to promote the use of public transport. This approach was based on factors described in the following document： ＂Urban Sustainability： Impacts of Economic Development and Their Consequences on the Process of Urbanization in Emerging Countries＂, prepared by the United Nations. The results indicate different planning strategies, ranging from focusing on tax integration policies in order to become more financially attractive to the user, to using different modes of mass transportation integrated with active mobility. In Washington D.C., an innovative strategy was developed in preparation to forecast scenarios, as the measures comprised projection for the year 2040, which will respond to mobility demands in the coming decades.
文摘Yakima County, Washington, a rural county with an urban core suffered disproportionately under the conditions presented by the COVID-19 pandemic and summer wildfires of 2020. With an infection rate of over 700 per 100,000 population at the height of the pandemic, the county concurrently experienced 14 consecutive days of an air quality index in the unhealthy to hazardous range in August 2020. This paper examines the contributing socioeconomic, geographic, and environmental vulnerabilities that make Yakima County particularly susceptible to the continuum of expected COVID-19 disease and related outcomes and suggests comprehensive areas of investigation to mitigate its impact on special populations, including Hispanic-Latino communities, agricultural, food production, and other essential workers.
文摘Vegetation and soil within estuarine ecosystems play an integral role in ecological processes within pocket estuaries. However, physical barriers, caused by culverts diminish hydrological inputs, sediment exchange, and habitat connectivity. The restoration of estuaries by bridge replacement reconnects <span>the aquatic corridor, however, the recovery of plant communities and soil s</span>ubstrate is not well understood. This observational study monitored four estuary restoration sites of variable ages (0 - 12 years) in Western Washington, USA. Plant community composition, soil organic carbon, organic matter, and soil nutrients were assessed. Percent soil carbon was different among the pre-restoration and youngest (3-year) post-restoration site (<i>P</i> = 0.03), suggesting an initial decrease in carbon and organic matter during restoration. Both N and P were deficient at the newest, lower restoration site, presumably linked to the lack of organic matter required for adequate cation exchange capacity and nutrient/plant exchange (<i>P</i> < 0.05). Plant species diversity was higher at the intermediate (9-year) and oldest post-restoration sites (12-year;<i>P</i> = 0.02). Vegetation composition was primarily native species with few invasive plants present. The results of this study illustrate that tidal marsh plant communities are influenced by the development of salinity and vertical gradients with older sites having an increase in species diversity. Future surveys are ongoing to <span>better understand how these sites recover organic matter and tidal marsh co</span>mmunities to form adequate estuarine habitat over time.
文摘The University of Washington has played a pivotal role in the field of sinology with faculty and alumni producing major publications in Chinese history,literature,phonetics,and linguistics.These contributions have been instrumental in the development of sinology as a field and have both directly and indirectly influenced the study of Chinese Medicine.By tracing the history of the Department of Asian Languages and Literature and examining several major figures,we can better understand how these individuals shaped the development of Chinese Medicine and contributed to its spread worldwide.
文摘In the United States, type 2 diabetes mellitus (T2DM) disproportionately affects the African American (AA) community, which has not been systematically included in molecular studies of underlying mechanisms. As part of a gene expression study, we recruited cases with T2DM and matched, unaffected controls at an urban hospital in Washington, DC, with a majority AA population. Here we describe the profile of socio-demographic, behavioral, and health-related associations of the study population. Self-reported data were collected from cases with T2DM (N = 77) and age- and gender-matched controls (N = 80), ages 45 - 65 years. Logistic regression was used to calculate odds ratios (OR) and 95% confidence intervals (CI). As expected, obesity, hypertension, and cardiovascular disease were more prevalent in cases than in controls. Tobacco smoking and working alongside other tobacco smokers were also associated with T2DM. After adjusting for covariates, current tobacco smoking remained statistically associated with the disease (OR per half pack of cigarettes 1.43, 95% CI 1.04 - 1.95;p-value 0.027). HbA1c levels were elevated in T2DM cases who smoked more than a pack of cigarettes daily. These associations highlight the comorbid burdens of T2DM in an AA urban community setting and identify tobacco control as an unmet need for future prevention and control efforts.
基金The authors would like thank S. Schveilbein and R. Wight for technical assistance G. Reisenauer and R. Aldridge for statistical assistance+2 种基金 B. Parker for plant identification assistance C. Looney for arthropod identification assistance and S. O＇Neal for editorial feedback. The authors are grateful to Olson Brothers Inc. for the use of their property. The authors would like to acknowledge the National Science Foundation Center for Integrated Pest Management, The Washington State Tree Fruit Research Commission, and the Washington State Commission on Pesticide Registration for financial support.
文摘Due to federal, state, and local regulations pertaining to clean water and salmon recovery, stream health and water quality are major concerns in the Pacific Northwest of the United States. An integral component of most riparian rehabilitation efforts is the effective establishment of preferred vegetation. We conducted arthropod surveys in representative disturbed and rehabilitated riparian buffers directly adjacent to apple orchards and grape vineyards in the Lower Yakima Valley near Prosser, Benton County, Washington State USA. Objectives of the study were to determine whether densities of select predatory and pest taxa changed with distance from the waterway and to test whether densities differed among disturbed, rehabilitated, or pristine riparian buffers. The studies suggest that populations of some beneficial arthropods, including spiders and ground beetles, were higher in the presence of exotic flowering plant species. In the riparian environments surveyed, more native woody plant species were found adjacent to the waterway, and this apparent stability appears to have had a positive effect on beneficial arthropods. Concurrently, data indicated that some exotic, flowering species of plants may be preferred over native plant species as hosts to certain arthropod pests. These plants tend to persist in degraded riparian buffers and at the interface of riparian habitats and cultivated areas, whereas native plant species are predominant in pristine or properly maintained and rehabilitated buffers.
文摘The East Asia Library(EAZ), following the leadership of the University of Washington(UW) Libraries and the trends of the library world, has made great progress in library automation. Yet the presence of non-roman scripts, or CJK characters, in the library's electronic resources in an environment dominated by phonetic languages makes automation of East Asian Libraries more challenging than libraries which use only roman scripts. This paper first provides a general overview of current information technology. It then offers a brief introduction to the UW Library automation and how the East Asia Library handles automation Finally, it presents the experience of the information technology in Chinese studies at EAL.
文摘Spatiotemporal analysis of fire activity is vital for determining why wildfires occur where they do,assessing wildfire risks,and developing locally relevant wildfire risk reduction strategies.Using various spatial statistical methods,we determined hot spots of large wildfires(>100 acres)in Washington,the United States,and mapped spatiotemporal variations in large wildfire activity from 1970 to 2020.Our results found that all hot spots are located east of the crest of the Cascade Range.Our spatiotemporal analysis found that the geographic area wherein most of the state’s acres burned has shrunk considerably since 1970 and has become concentrated over the north-central portion of the state over time.This concentration of large wildfire activity in north-central Washington was previously unquantified and may provide important information for hazard mitigation efforts in that area.Our results highlight the advantages of using spatial statistical methods that could aid the development of natural hazard mitigation plans and risk reduction strategies by characterizing previous hazard occurrences spatially and spatiotemporally.
文摘Regenerative capacity of the central nervous system(CNS)is unevenly distributed among vertebrates.While most mammalian species including humans elicit limited repair following CNS injury or disease,highly regenerative vertebrates including urodele amphibians and teleost fish spontaneously reverse CNS damage.Teletost zebrafish(danio rerio)are tropical freshwater fish that proved to be an excellent vertebrate model of successful CNS regeneration.Differential neuronal,glial,and immune injury responses underlie disparate injury outcomes between highly regenerative zebrafish and poorly regenerative mammals.This article describes complications associated with neuronal repair following spinal cord injury(SCI)in poorly regenerative mammals and highlights intersecting modes of plasticity and regeneration in highly regenerative zebrafish(Figures 1 and 2).Comparative approaches evaluating immunoglial SCI responses were recently reviewed elsewhere(Reyes and Mokalled,2024).
文摘Calcium (Ca^(2+)) is a key intracellular messenger involved in a variety of cellular functions.Intracellular Ca^(2+)dysregulation drives neuron cell death in multiple degenerative diseases and traumatic conditions.Retinal ganglion cell(RGC) degeneration occurs in blinding diseases such as glaucoma and other optic neuropathies.
基金sponsored by the Department of Defense,Defense Threat Reduction Agency under the Materials Science in Extreme Environments University Research Alliance,HDTRA1-20-2-0001。
文摘Chemical warfare agents(CWAs)remain a persistent hazard in many parts of the world,necessitating a deeper exploration of their chemical and physical characteristics and reactions under diverse conditions.Diisopropyl methylphosphonate(DIMP),a commonly used CWA surrogate,is widely studied to enhance our understanding of CWA behavior.The prevailing thermal decomposition model for DIMP,developed approximately 25 years ago,is based on data collected in nitrogen atmospheres at temperatures ranging from 700 K to 800 K.Despite its limitations,this model continues to serve as a foundation for research across various thermal and reactive environments,including combustion studies.Our recent experiments have extended the scope of decomposition analysis by examining DIMP in both nitrogen and zero air across a lower temperature range of 175℃ to 250℃.Infrared spectroscopy results under nitrogen align well with the established model;however,we observed that catalytic effects,stemming from decomposition byproducts and interactions with stainless steel surfaces,alter the reaction kinetics.In zero air environments,we observed a novel infrared absorption band.Spectral fitting suggests this band may represent a combination of propanal and acetone,while GCMS analysis points to vinyl formate and acetone as possible constituents.Although the precise identity of these new products remains unresolved,our findings clearly indicate that the existing decomposition model cannot be reliably extended to lower temperatures or non-nitrogen environments without further revisions.
基金Institutional National Research Service Award T32 EY013360(to SM and SW)Research to Prevent Blindness(Career Development Award+8 种基金to PRW)BrightFocus Foundation(National Glaucoma Researchto PRW)Alcon Research Institute(Young Investigator Awardto PRW),and NIH Grants(EY032908,EY035684,EY036111to PRW)the Jefferey T.Fort Innovation Fund and Siteman Retina Research Fund(to RSA)the Hope Center Viral Vectors Core at Washington University School of Medicine,an unrestricted grant(to the Department of Ophthalmology and Visual Sciences)from Research to Prevent BlindnessVision Core Grant(P30 EY002687).
文摘Retinal ganglion cells are susceptible to neurodegenerative conditions and their death drives common forms of irreversible vision loss.In mice,there are 46 transcriptionally unique retinal ganglion cell types that demonstrate different susceptibilities to degeneration.Recent transcriptional experiments defined a novel retinal ganglion cell type that survives particularly well and uniquely expresses high levels of the orphan G-protein-coupled receptor 88.Motivated to study this retinal ganglion cell type,we obtained GPR88-Cre transgenic mice to identify the novel well-surviving retinal ganglion cells and examine their survival and regenerative potential.Our experiments demonstrate that this unidentified retinal ganglion cell type is likely accordant with previously described ON-direction-selective retinal ganglion cells.Interestingly,we find that ON-direction-selective retinal ganglion cells are resilient,but demonstrate limited potential to regenerate their axons in response to well-characterized regenerative treatments.Studying the molecular properties of the ON-direction-selective retinal ganglion cells could unlock new therapeutics to preserve retinal ganglion cells in patients.
文摘Early and accurate detection of bone cancer and marrow cell abnormalities is critical for timely intervention and improved patient outcomes.This paper proposes a novel hybrid deep learning framework that integrates a Convolutional Neural Network(CNN)with a Bidirectional Long Short-Term Memory(BiLSTM)architecture,optimized using the Firefly Optimization algorithm(FO).The proposed CNN-BiLSTM-FO model is tailored for structured biomedical data,capturing both local patterns and sequential dependencies in diagnostic features,while the Firefly Algorithm fine-tunes key hyperparameters to maximize predictive performance.The approach is evaluated on two benchmark biomedical datasets:one comprising diagnostic data for bone cancer detection and another for identifying marrow cell abnormalities.Experimental results demonstrate that the proposed method outperforms standard deep learning models,including CNN,LSTM,BiLSTM,and CNN-LSTM hybrids,significantly.The CNNBiLSTM-FO model achieves an accuracy of 98.55%for bone cancer detection and 96.04%for marrow abnormality classification.The paper also presents a detailed complexity analysis of the proposed algorithm and compares its performance across multiple evaluation metrics such as precision,recall,F1-score,and AUC.The results confirm the effectiveness of the firefly-based optimization strategy in improving classification accuracy and model robustness.This work introduces a scalable and accurate diagnostic solution that holds strong potential for integration into intelligent clinical decision-support systems.
基金supported by the Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A04013)the National Natural Science Foundation of China(82204610)+1 种基金the Qihang Talent Program(L2022046)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-041 and L2021029).
文摘Background:The medicinal material known as Os Draconis(Longgu)originates from fossilized remains of ancient mammals and is widely used in treating emotional and mental conditions.However,fossil resources are nonrenewable,and clinical demand is increasingly difficult to meet,leading to a proliferation of counterfeit products.During prolonged geological burial,static pressure from the surrounding strata severely compromises the microstructural integrity of osteons in Os Draconis,but Os Draconis still largely retains the structural features of mammalian bone.Methods:Using verified authentic Os Draconis samples over 10,000 years old as a baseline,this study summarizes the ultrastructural characteristics of genuine Os Draconis.Employing electron probe microanalysis and optical polarized light microscopy,we examined 28 batches of authentic Os Draconis and 31 batches of counterfeits to identify their ultrastructural differences.Key points for ultrastructural identification of Os Draconis were compiled,and a new identification approach was proposed based on these differences.Results:Authentic Os Draconis exhibited distinct ultrastructural markers:irregularly shaped osteons with traversing fissures,deformed/displaced Haversian canals,and secondary mineral infill(predominantly calcium carbonate).Counterfeits showed regular osteon arrangements,absent traversal fissures,and homogeneous hydroxyapatite composition.Lab-simulated samples lacked structural degradation features.EPMA confirmed calcium carbonate infill in fossilized Haversian canals,while elemental profiles differentiated lacunae types(void vs.mineral-packed).Conclusion:The study established ultrastructural criteria for authentic Os Draconis identification:osteon deformation,geological fissures penetrating bone units,and heterogenous mineral deposition.These features,unattainable in counterfeits or modern processed bones,provide a cost-effective,accurate identification method.This approach bridges gaps in TCM material standardization and supports quality control for clinical applications.
