The prevalence of bacterial digestive diseases in plateau animals has caused considerable losses to the Tibetan live‑stock industry.Therefore,this study aimed to isolate safe lactic acid bacteria(LAB)with beneficial p...The prevalence of bacterial digestive diseases in plateau animals has caused considerable losses to the Tibetan live‑stock industry.Therefore,this study aimed to isolate safe lactic acid bacteria(LAB)with beneficial probiotic proper‑ties to protect yaks from intestinal diseases.After 16S rDNA matching,four strains of Lactobacillus fermentum(A4),Pediococcus pentosaceus(A3.4 and A1.2),and Pediococcus acidilactici(B1.9)were isolated from the intestinal tissues and content of healthy yaks.The results indicated that A4 was more tolerant to bile salt(0.3%),while A3.4 had better stability in an acidic(pH=3.0)environment.The results of the antibacterial activity test suggested that the isolates inhibited most pathogenic bacteria by up to 20%,except for A3.4,which inhibited Pasteurella and Staphylococcus aureus by more than 20%.Moreover,the results of the antioxidant test demonstrated that A4 and A3.4 had potent antioxidant activity.In addition,the drug sensitivity test revealed that the isolates were susceptible to commonly used antibiotics.In terms of safety,the isolates promoted growth,enhanced intestinal development,and protected the intestinal barrier without causing any adverse effects.In conclusion,LAB isolated from yak intestinal contents are potential probiotics with excellent antibacterial properties.展开更多
Brucellosis,a zoonotic disease caused by Brucella infection,poses a major threat to both global health and livestock productivity.Although reproductive impairment is well established,the molecular mechanisms driving t...Brucellosis,a zoonotic disease caused by Brucella infection,poses a major threat to both global health and livestock productivity.Although reproductive impairment is well established,the molecular mechanisms driving testicular immunopathology remain poorly understood.In this study,single-cell RNA sequencing was used to delineate transcriptional changes in goat testicular tissues under physiological and Brucella-infected conditions,revealing dynamic immunological remodeling of the testicular microenvironment.Infection induced marked shifts in T cell and macrophage phenotypes,with T cells exhibiting pronounced hyperactivation linked to CD45-mediated signaling cascades.Thioredoxin-interacting protein(TXNIP),a gene strongly up-regulated in response to infection,emerged as a potential immunotherapeutic target.Intercellular communication networks were significantly disrupted in infected testes,with CD39-and JAM-dependent signaling pathways implicated in the erosion of immune privilege.Regulon analysis further identified GATA3,IRF5,SEMA4A,and HCLS1 as transcriptional regulators associated with T cells and macrophages in infected testes.These findings provide novel insights into the molecular mechanisms driving testicular immunopathology during Brucella infection and highlight candidate targets for immunomodulatory intervention in disease control and livestock reproductive health.展开更多
Objective:To evaluate activity of methanol extract of Achillea fragrantissima(meth)(A.fragrantissima) alone or in combination with diminazine aceturate(DA) against Trypanosoma evansi in experimentally infected rats.Me...Objective:To evaluate activity of methanol extract of Achillea fragrantissima(meth)(A.fragrantissima) alone or in combination with diminazine aceturate(DA) against Trypanosoma evansi in experimentally infected rats.Methods:Sixty adult male Wister albino rats were divided equally into 6 groups(A-F).Rats in groups A-E were experimentally infected with T.evansi and those in group F were uninfected.The groups were treated respectively as follows:group A- with 3.5 mg/kg DA;group B- with 1 000 mg/kg meth,A.fragrantissima;group C-3.5mg/kg DA plus 500 mg/kg meth A.fragrantissima;group D-3.5 mg/kg DA plus 1 000 mg/kg meth A.fragrantissima.Group E was left untreated.Parasitaemia,survivability,packed cell volume,hemoglobin concentration,total leucocytes count,lymphocyte count,and serum malondialdehyde and reduced glutathione(GSH) levels were estimated.Phytochemical screening of meth A.fragrantissima was also performed.Results:The phytochemical analysis of the meth A.fragrantissima indicated a higher content from polyphenols tannins and non tannins and flavonoids.The efficacy percentage against trypanosomiasis in groups A to E was respectively as follows 80,40,90.100,0.The administration of meth-A.fragrantissima(1000)mg/kg b.wt.) produced a moderate efficacy against trypanosomiasis.Untreated rats in group E died between 25 and 30 d post infection.The rats given DA and meth A.fragrantissima combinations(C and D) showed faster and higher recovery rates than the uninfected control and groups A and B.The initial reduction in packed cell volume,hemoglobin,total leucocytes count,increases in serum malondialdehyde and decreases in GSH levels were reversed by the treatments.C onclusions:The administration of the methanol extracts of A.fragrantissima and DA combination therapy was more effective than each product alone in the treatment of rats infected with Trypanosoma evansi and further studies are required to isolate more active ingredients.展开更多
[Objectives]To study the effects of different alkaloids in Coptis chinensis on inhibiting the proliferation of Transmissible gastroenteritis virus(TGEV).[Methods]The components and content of the main alkaloids in the...[Objectives]To study the effects of different alkaloids in Coptis chinensis on inhibiting the proliferation of Transmissible gastroenteritis virus(TGEV).[Methods]The components and content of the main alkaloids in the extract of C.chinensis were analyzed.The main alkaloids were selected as drugs to inhibit the proliferation of TGEV.The maximum non-toxic concentration of Columbamine,Jatrorrhizine,Epiberberine,Coptisine,Palmatine,and Berberine was screened.The protective rate of each drug on TGEV-infected ST cells was determined,and the transcriptional inhibitory effect of the drug on TGEV N gene was detected by fluorescent quantitative PCR.[Results]The extract of C.chinensis mainly contains 6 alkaloids:Columbamine,Jatrorrhizine,Epiberberine,Coptisine,Palmatine,and Berberine,accounting for 2.03%,8.88%,9.21%,15.07%,14.63%,and 50.18%,respectively.In the range of the safe concentration,Jatrorrhizine,Palmatine,and Coptisine had better protective effects on ST cells infected with TGEV;compared with the Columbamine group,the cell protection rate was significantly different(P<0.05);compared with the Berberine group,the difference was extremely significant(P<0.01).The Coptisine and Palmatine groups had significant inhibitory effects on the transcription of TGEV N gene,and the difference was extremely significant compared with the virus group(P<0.05).[Conclusions]Jatrorrhizine and Palmatine in C.chinensis are the main components to inhibit the proliferation of TGEV.展开更多
This study aimed to evaluate the effects of encapsulated benzoic acid(EBA)on alleviating lipopolysaccharide(LPS)-induced intestinal inflammation and barrier dysfunction.EBA was developed through monoglyceridebased mic...This study aimed to evaluate the effects of encapsulated benzoic acid(EBA)on alleviating lipopolysaccharide(LPS)-induced intestinal inflammation and barrier dysfunction.EBA was developed through monoglyceridebased microencapsulation,which enhances the stability of benzoic acid(BA)under gastric conditions and facilitates targeted release in the intestine.The effects of EBA were evaluated in vitro using LPS-treated IPEC-J2 cells,assessing inflammatory cytokine production,tight junction integrity,and TLR4/NF-κB pathway activation.The impact of EBA on intestinal inflammation,barrier function,tissue morphology,and nuclear factor erythroid 2-related factor 2(NRF2)activation was evaluated in LPS-challenged ICR mice.EBA significantly reduced inflammatory cytokine levels(TNF-α,IL-1β,and IL-8;P<0.05),suppressed the TLR4/NF-κB signaling pathway(P<0.05),and restored tight junction protein expression(ZO-1,OCLN,CLDN1,and CLDN3;P<0.05)compared to LPS treatment alone.Furthermore,EBA demonstrated superior efficacy over unencapsulated BA(P<0.05).Inhibition of NRF2 abolished the protective effects of EBA(P<0.05),suggesting that NRF2 activation is crucial for mediating these beneficial effects.These findings support the potential of EBA as a novel dietary intervention to alleviate intestinal inflammation and barrier dysfunction,providing new insights for maintaining gut health in animals and humans.展开更多
基金supported by the Key Project of Tibet Autonomous Region(XZ202001ZY0044NXZ202101ZD0002N-05).
文摘The prevalence of bacterial digestive diseases in plateau animals has caused considerable losses to the Tibetan live‑stock industry.Therefore,this study aimed to isolate safe lactic acid bacteria(LAB)with beneficial probiotic proper‑ties to protect yaks from intestinal diseases.After 16S rDNA matching,four strains of Lactobacillus fermentum(A4),Pediococcus pentosaceus(A3.4 and A1.2),and Pediococcus acidilactici(B1.9)were isolated from the intestinal tissues and content of healthy yaks.The results indicated that A4 was more tolerant to bile salt(0.3%),while A3.4 had better stability in an acidic(pH=3.0)environment.The results of the antibacterial activity test suggested that the isolates inhibited most pathogenic bacteria by up to 20%,except for A3.4,which inhibited Pasteurella and Staphylococcus aureus by more than 20%.Moreover,the results of the antioxidant test demonstrated that A4 and A3.4 had potent antioxidant activity.In addition,the drug sensitivity test revealed that the isolates were susceptible to commonly used antibiotics.In terms of safety,the isolates promoted growth,enhanced intestinal development,and protected the intestinal barrier without causing any adverse effects.In conclusion,LAB isolated from yak intestinal contents are potential probiotics with excellent antibacterial properties.
基金supported by the National Key Research and Development Program of China (2023YFF1000904,2022YFD1302201)National Natural Science Foundation of China(32372970, U24A20438)+2 种基金Key Technologies Demonstration of Animal Husbandry in Shaanxi Province (2025NYGG005, 2024NYGG005)Inner Mongolia Autonomous Region Open Competition Projects(2022JBGS0025)Open Fund Project of the National Key Laboratory of Veterinary Public Health and Safety (2025SKLVPHS08)。
文摘Brucellosis,a zoonotic disease caused by Brucella infection,poses a major threat to both global health and livestock productivity.Although reproductive impairment is well established,the molecular mechanisms driving testicular immunopathology remain poorly understood.In this study,single-cell RNA sequencing was used to delineate transcriptional changes in goat testicular tissues under physiological and Brucella-infected conditions,revealing dynamic immunological remodeling of the testicular microenvironment.Infection induced marked shifts in T cell and macrophage phenotypes,with T cells exhibiting pronounced hyperactivation linked to CD45-mediated signaling cascades.Thioredoxin-interacting protein(TXNIP),a gene strongly up-regulated in response to infection,emerged as a potential immunotherapeutic target.Intercellular communication networks were significantly disrupted in infected testes,with CD39-and JAM-dependent signaling pathways implicated in the erosion of immune privilege.Regulon analysis further identified GATA3,IRF5,SEMA4A,and HCLS1 as transcriptional regulators associated with T cells and macrophages in infected testes.These findings provide novel insights into the molecular mechanisms driving testicular immunopathology during Brucella infection and highlight candidate targets for immunomodulatory intervention in disease control and livestock reproductive health.
基金This project(No.BCS06)was financiully supported by Promising Research Center in Biological Control and Agricultural Information(BCARC).Qassim University,Al Qassim.Kingdom of Saudi Arabia
文摘Objective:To evaluate activity of methanol extract of Achillea fragrantissima(meth)(A.fragrantissima) alone or in combination with diminazine aceturate(DA) against Trypanosoma evansi in experimentally infected rats.Methods:Sixty adult male Wister albino rats were divided equally into 6 groups(A-F).Rats in groups A-E were experimentally infected with T.evansi and those in group F were uninfected.The groups were treated respectively as follows:group A- with 3.5 mg/kg DA;group B- with 1 000 mg/kg meth,A.fragrantissima;group C-3.5mg/kg DA plus 500 mg/kg meth A.fragrantissima;group D-3.5 mg/kg DA plus 1 000 mg/kg meth A.fragrantissima.Group E was left untreated.Parasitaemia,survivability,packed cell volume,hemoglobin concentration,total leucocytes count,lymphocyte count,and serum malondialdehyde and reduced glutathione(GSH) levels were estimated.Phytochemical screening of meth A.fragrantissima was also performed.Results:The phytochemical analysis of the meth A.fragrantissima indicated a higher content from polyphenols tannins and non tannins and flavonoids.The efficacy percentage against trypanosomiasis in groups A to E was respectively as follows 80,40,90.100,0.The administration of meth-A.fragrantissima(1000)mg/kg b.wt.) produced a moderate efficacy against trypanosomiasis.Untreated rats in group E died between 25 and 30 d post infection.The rats given DA and meth A.fragrantissima combinations(C and D) showed faster and higher recovery rates than the uninfected control and groups A and B.The initial reduction in packed cell volume,hemoglobin,total leucocytes count,increases in serum malondialdehyde and decreases in GSH levels were reversed by the treatments.C onclusions:The administration of the methanol extracts of A.fragrantissima and DA combination therapy was more effective than each product alone in the treatment of rats infected with Trypanosoma evansi and further studies are required to isolate more active ingredients.
基金Supported by General Project of Chongqing Natural Science Foundation(cstc2020jcyj-msxmX0757)Pilot Project of National Center of Technology Innovation for Pigs(NCTIP-XD/B12)Regional Innovation Cooperation in Sichuan Province(2022YFQ0024)。
文摘[Objectives]To study the effects of different alkaloids in Coptis chinensis on inhibiting the proliferation of Transmissible gastroenteritis virus(TGEV).[Methods]The components and content of the main alkaloids in the extract of C.chinensis were analyzed.The main alkaloids were selected as drugs to inhibit the proliferation of TGEV.The maximum non-toxic concentration of Columbamine,Jatrorrhizine,Epiberberine,Coptisine,Palmatine,and Berberine was screened.The protective rate of each drug on TGEV-infected ST cells was determined,and the transcriptional inhibitory effect of the drug on TGEV N gene was detected by fluorescent quantitative PCR.[Results]The extract of C.chinensis mainly contains 6 alkaloids:Columbamine,Jatrorrhizine,Epiberberine,Coptisine,Palmatine,and Berberine,accounting for 2.03%,8.88%,9.21%,15.07%,14.63%,and 50.18%,respectively.In the range of the safe concentration,Jatrorrhizine,Palmatine,and Coptisine had better protective effects on ST cells infected with TGEV;compared with the Columbamine group,the cell protection rate was significantly different(P<0.05);compared with the Berberine group,the difference was extremely significant(P<0.01).The Coptisine and Palmatine groups had significant inhibitory effects on the transcription of TGEV N gene,and the difference was extremely significant compared with the virus group(P<0.05).[Conclusions]Jatrorrhizine and Palmatine in C.chinensis are the main components to inhibit the proliferation of TGEV.
基金supported by the Natural Science Foundation of Jiangsu Province(BK20241566)Fundamental Research Funds for the Central University(YDZX2024031)Chinese Postdoctoral Science Foundation(2022M721646).
文摘This study aimed to evaluate the effects of encapsulated benzoic acid(EBA)on alleviating lipopolysaccharide(LPS)-induced intestinal inflammation and barrier dysfunction.EBA was developed through monoglyceridebased microencapsulation,which enhances the stability of benzoic acid(BA)under gastric conditions and facilitates targeted release in the intestine.The effects of EBA were evaluated in vitro using LPS-treated IPEC-J2 cells,assessing inflammatory cytokine production,tight junction integrity,and TLR4/NF-κB pathway activation.The impact of EBA on intestinal inflammation,barrier function,tissue morphology,and nuclear factor erythroid 2-related factor 2(NRF2)activation was evaluated in LPS-challenged ICR mice.EBA significantly reduced inflammatory cytokine levels(TNF-α,IL-1β,and IL-8;P<0.05),suppressed the TLR4/NF-κB signaling pathway(P<0.05),and restored tight junction protein expression(ZO-1,OCLN,CLDN1,and CLDN3;P<0.05)compared to LPS treatment alone.Furthermore,EBA demonstrated superior efficacy over unencapsulated BA(P<0.05).Inhibition of NRF2 abolished the protective effects of EBA(P<0.05),suggesting that NRF2 activation is crucial for mediating these beneficial effects.These findings support the potential of EBA as a novel dietary intervention to alleviate intestinal inflammation and barrier dysfunction,providing new insights for maintaining gut health in animals and humans.
