Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length ...Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus digitally from the base of the penis was performed on four adult male crocodiles to facilitate the flow of semen to the sulcus. Semen was collected from all individuals for spermatozoa morphometry and ultrastructure study. Morphometry analysis was performed from eosin-nigrosin stained spermatozoa sample. Scanning electron microscopy was conducted to observe the surface ultrastructure of spermatozoa. Results: The morphology of crocodile spermatozoa was made up of acrosome, head, and tail which corresponded to (5.55±1.20) μm, (12.74±1.57) μm, and (70.67±4.40) μm, respectively. The total length of spermatozoa in estuarine crocodile was measured at (88.96±0.52) μm. The most common spermatozoa abnormalities found in the samples were cytoplasmic droplets. Conclusions: Normal morphology, morphometry of spermatozoa in estuarine crocodile which are vermiform shaped-head with a long tail along with questionably high cytoplasmic droplets count are described in this study.展开更多
The aim of the present study was to estimate the seroprevalence of antibodies to bluetongue virus (BTV) among domestic ruminants of Grenada. Sera samples from cattle (133), goat (314) and sheep (481) were tested using...The aim of the present study was to estimate the seroprevalence of antibodies to bluetongue virus (BTV) among domestic ruminants of Grenada. Sera samples from cattle (133), goat (314) and sheep (481) were tested using competitive Enzyme-linked immunosorbent assay (c-ELISA). Of the total of 928 samples tested, the overall BTV seroprevalence was 78.4% (95% confidence interval (CI ± 2.65). The seropositivity of ovine, caprine and bovine was found to be 71.7% (95% CI, 67.67% to 75.73%), 80.2% (95% CI, 75.79% to 84.61%) and 98.5% (95% CI, 96.43% to 100.57%), respectively. There was statistical significance in the seroprevalence of BTV among bovine, caprine and ovine (p < 0.05). It is evident from this study that blue tongue virus is endemic in Grenada.展开更多
Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods...Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques,kept in a cool box and sent to laboratory for analysis.The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers(ABX ABC Vet,Horiba ABX SAS^(®)Montpellier,France).Results:Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females.Aspartate aminotransferase values were higher(P<0.05)in males compared to female cats.Serum protein,creatinine and glucose values for females were significantly(P<0.05)higher than those for males.Creatinine kinase,urea and lactate dehydrogenase levels were higher than those reported for normal domestic cat,but there was no statistical difference(P>0.05)between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats.These values will be important for diagnosis of various diseases and monitoring of treatments.展开更多
文摘Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus digitally from the base of the penis was performed on four adult male crocodiles to facilitate the flow of semen to the sulcus. Semen was collected from all individuals for spermatozoa morphometry and ultrastructure study. Morphometry analysis was performed from eosin-nigrosin stained spermatozoa sample. Scanning electron microscopy was conducted to observe the surface ultrastructure of spermatozoa. Results: The morphology of crocodile spermatozoa was made up of acrosome, head, and tail which corresponded to (5.55±1.20) μm, (12.74±1.57) μm, and (70.67±4.40) μm, respectively. The total length of spermatozoa in estuarine crocodile was measured at (88.96±0.52) μm. The most common spermatozoa abnormalities found in the samples were cytoplasmic droplets. Conclusions: Normal morphology, morphometry of spermatozoa in estuarine crocodile which are vermiform shaped-head with a long tail along with questionably high cytoplasmic droplets count are described in this study.
文摘The aim of the present study was to estimate the seroprevalence of antibodies to bluetongue virus (BTV) among domestic ruminants of Grenada. Sera samples from cattle (133), goat (314) and sheep (481) were tested using competitive Enzyme-linked immunosorbent assay (c-ELISA). Of the total of 928 samples tested, the overall BTV seroprevalence was 78.4% (95% confidence interval (CI ± 2.65). The seropositivity of ovine, caprine and bovine was found to be 71.7% (95% CI, 67.67% to 75.73%), 80.2% (95% CI, 75.79% to 84.61%) and 98.5% (95% CI, 96.43% to 100.57%), respectively. There was statistical significance in the seroprevalence of BTV among bovine, caprine and ovine (p < 0.05). It is evident from this study that blue tongue virus is endemic in Grenada.
基金supported by management of Al Ain Wildlife Park and Resort(Grant No.12/917002).
文摘Objective:To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort,United Arab Emirates,with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques,kept in a cool box and sent to laboratory for analysis.The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers(ABX ABC Vet,Horiba ABX SAS^(®)Montpellier,France).Results:Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females.Aspartate aminotransferase values were higher(P<0.05)in males compared to female cats.Serum protein,creatinine and glucose values for females were significantly(P<0.05)higher than those for males.Creatinine kinase,urea and lactate dehydrogenase levels were higher than those reported for normal domestic cat,but there was no statistical difference(P>0.05)between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats.These values will be important for diagnosis of various diseases and monitoring of treatments.
