This paper presents a new silicon-on-insulator(SOI) lateral-double-diffused metal-oxide-semiconductor transistor(LDMOST) device with alternated high-k dielectric and step doped silicon pillars(HKSD device). Due to the...This paper presents a new silicon-on-insulator(SOI) lateral-double-diffused metal-oxide-semiconductor transistor(LDMOST) device with alternated high-k dielectric and step doped silicon pillars(HKSD device). Due to the modulation of step doping technology and high-k dielectric on the electric field and doped profile of each zone, the HKSD device shows a greater performance. The analytical models of the potential, electric field, optimal breakdown voltage, and optimal doped profile are derived. The analytical results and the simulated results are basically consistent, which confirms the proposed model suitable for the HKSD device. The potential and electric field modulation mechanism are investigated based on the simulation and analytical models. Furthermore, the influence of the parameters on the breakdown voltage(BV) and specific on-resistance(R_(on,sp)) are obtained. The results indicate that the HKSD device has a higher BV and lower R_(on,sp) compared to the SD device and HK device.展开更多
The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA)....The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.展开更多
The experimental solubility of CBZ (Carbobenzoxy) derivatized amino acids namely N-CBZ valine, N-CBZ proline, N-CBZ aspartic acid in supercritical carbon dioxide were correlated by Soave-Red- lich-Kwong Equation state...The experimental solubility of CBZ (Carbobenzoxy) derivatized amino acids namely N-CBZ valine, N-CBZ proline, N-CBZ aspartic acid in supercritical carbon dioxide were correlated by Soave-Red- lich-Kwong Equation state based on fugacity determination and group contribution method using extrapolated critical parameters and mixing rules and other two different empirical models proposed by Yu (1994) and Gordillo-coworkers (1999). The SRK EOS prediction showed very high deviation of % AARD of 9% - 59%. The Yu model had three derivatized amino acids with average absolute deviation from 2.04, 8.17, 10.96, while the Gordillo model had 0.245, 1.067 and 1.144 for CBZ-valine, CBZ-proline and CBZ-aspartic acid successively. The correlated values had better fit with Gordillo model. The predictive capability and applicability for these amino acid derivatives for both the models demonstrated with correlation coefficient around 0.99 for all the experimental solubility observed.展开更多
Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiatio...Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.展开更多
The ability of some nanostructured materials to perform as effective heterogeneous catalysts is potentially hindered by the failure of the liquid reactant to effectively wet the solid catalyst surface. In this work, t...The ability of some nanostructured materials to perform as effective heterogeneous catalysts is potentially hindered by the failure of the liquid reactant to effectively wet the solid catalyst surface. In this work, two different chemical reactions, each involving a change of phase from liquid to gas on a solid catalyst surface, are investigated. The first reaction is the catalyzed decomposition of a H<sub>2</sub>O<sub>2</sub> monopropellant within a micro-chemical reactor chamber, decorated with RuO<sub>2</sub> nanorods (NRs). The second reaction involves the electrolysis of dilute aqueous solutions of H<sub>2</sub>SO<sub>4</sub> performed with the cathode electrode coated with different densities and sizes of RuO<sub>2</sub> NRs. In the catalyzed H<sub>2</sub>O<sub>2 </sub>decomposition, the reaction rate is observed to decrease with increasing catalyst surface density because of a failure of the liquid to wet on the catalyst surface. In the electrolysis experiment, however, the reaction rate increased in proportion to the surface density of RuO<sub>2</sub> NRs. In this case, the electrical bias applied to drive the electrolysis reaction also causes an electrostatic force of attraction between the fluid and the NR coated surface, and thus assures effective wetting.展开更多
Opaque polyploid cells capable of forming megamitochondria are a constant <span style="font-family:Verdana;">feature in colonies of Ishikawa endometrial epithelia, accounting for ap</span><spa...Opaque polyploid cells capable of forming megamitochondria are a constant <span style="font-family:Verdana;">feature in colonies of Ishikawa endometrial epithelia, accounting for ap</span><span style="font-family:Verdana;">proximately 5</span><span style="font-family:Verdana;">% </span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">10% of the cells. Opaque cells appear to communicate with other </span><span style="font-family:Verdana;">opaque cells via membrane extensions and with other cells in a colony by </span><span style="font-family:Verdana;">extra</span></span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">cellular vesicles. Opaque cells form first as rectangular structures, somewhat larger than surrounding monolayer cells. The cells eventually round up, re</span><span style="font-family:Verdana;">maining in the colony for 20 or more hours before detaching. The most </span><span style="font-family:Verdana;">un</span><span style="font-family:Verdana;">usual characteristic of Ishikawa opaque cells is their capacity to form mitonucleons, megamitochondria that surround aggregated chromatin. This</span><span style="font-family:Verdana;"> paper reviews evidence that adaptations resulting in megamitochondria i</span><span style="font-family:Verdana;">nclude a loss of the capacity for oxidative phosphorylation leaving the adapted </span><span style="font-family:Verdana;">megamitochondria reliant on metabolism such as reductive carboxylation.</span></span>展开更多
Pediatric autoimmune neuropsychiatric disorders associated with or without streptococcal and other bacterial infections (PANDAS/CANS) are emerging as a featured pediatric disorder. Although there is some controversy r...Pediatric autoimmune neuropsychiatric disorders associated with or without streptococcal and other bacterial infections (PANDAS/CANS) are emerging as a featured pediatric disorder. Although there is some controversy regarding treatment approaches, especially related to the behavioral sequelae, we have hypothesized in other published work that it is characterized by the rapid onset of Reward Deficiency Syndrome (RDS) in children. We propose utilizing a multi-systems biological approach involving the coupling of genetic addiction risk testing and pro-dopamine regulation (KB220/POLYGEN®) to help induce “dopamine homeostasis” in patients with PANDAS, especially those with known DNA-induced hypodopaminergia. This case study examines a 12-year-old Caucasian male with no prior psychiatric issues who presented with a sudden onset of severe anxiety, depression, emotional liability, and suicidal ideation. The patient underwent genotyping and the genetic addiction risk score (GARS) testing, which revealed risk polymorphisms in the dopamine D2 (-DRD2/ANKK (Taq1A), OPRM1 (A/G), DRD3 (C/T), and MAOA (4R) genes. These polymorphisms have been linked to hypodopaminergia. The patient was subsequently placed on research ID-KB220ZPBMPOLY (POLYGEN®), and albeit the possibility of bias, based upon self and parental assessment, a marked rapid improvement in psychiatric symptoms was observed. In the second phase of treatment (102 days utilizing KB220), the patient received standard antibody testing, which was positive for Lyme. Antibacterial therapy started immediately, and KB220z was discontinued to provide a wash-out period. A monotonic trend analysis was performed on each outcome measure, and a consistently decreasing trend was observed utilizing antibacterial therapy. Our recommendation, albeit only one case, is to utilize and further research a combined therapeutic approach, involving precision-guided DNA testing and pro-dopamine regulation along with antibacterial therapy, as well as glutathione to address offensive enhanced cytokines, in patients with suspected PANDAS/CANS.展开更多
As X-ray computed tomography （CT） is widely used in diagnosis and radiotherapy, it is important to reduce the radiation dose as low as reasonably achievable. For this pur- pose, one may use the TV based methods or w...As X-ray computed tomography （CT） is widely used in diagnosis and radiotherapy, it is important to reduce the radiation dose as low as reasonably achievable. For this pur- pose, one may use the TV based methods or wavelet frame based methods to reconstruct high quality images from reduced number of projections. Furthermore, by using the in- terior tomography scheme which only illuminates a region-of-interest （ROI）, one can save more radiation dose. In this paper, a robust wavelet frame regularization based model is proposed for both global reconstruction and interior tomography. The model can help to reduce the errors caused by mismatch of the huge sparse projection matrix. A three-system decomposition scheme is applied to decompose the reconstructed images into three differ- ent parts： cartoon, artifacts and noise. Therefore, by discarding the estimated artifacts and noise parts, the reconstructed images can be obtained with less noise and artifacts. Similar to other frame based image restoration models, the model can be efficiently solved by the split Bregman algorithm. Numerical simulations show that the proposed model outperforms the FBP and SART＋TV methods in terms of preservation of sharp edges, mean structural similarity （SSIM）, contrast-to-noise ratio, relative error and correlation- s. For example, for real sheep lung reconstruction, the proposed method can reach the mean structural similarity as high as 0.75 using only 100 projections while the FBP and the SART^TV methods need more than 200 projections. Additionally, the proposed ro- bust method is applicable for interior and exterior tomography with better performance compared to the FBP and the SART＋TV methods.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61704084 and 61874059)。
文摘This paper presents a new silicon-on-insulator(SOI) lateral-double-diffused metal-oxide-semiconductor transistor(LDMOST) device with alternated high-k dielectric and step doped silicon pillars(HKSD device). Due to the modulation of step doping technology and high-k dielectric on the electric field and doped profile of each zone, the HKSD device shows a greater performance. The analytical models of the potential, electric field, optimal breakdown voltage, and optimal doped profile are derived. The analytical results and the simulated results are basically consistent, which confirms the proposed model suitable for the HKSD device. The potential and electric field modulation mechanism are investigated based on the simulation and analytical models. Furthermore, the influence of the parameters on the breakdown voltage(BV) and specific on-resistance(R_(on,sp)) are obtained. The results indicate that the HKSD device has a higher BV and lower R_(on,sp) compared to the SD device and HK device.
文摘The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.
文摘The experimental solubility of CBZ (Carbobenzoxy) derivatized amino acids namely N-CBZ valine, N-CBZ proline, N-CBZ aspartic acid in supercritical carbon dioxide were correlated by Soave-Red- lich-Kwong Equation state based on fugacity determination and group contribution method using extrapolated critical parameters and mixing rules and other two different empirical models proposed by Yu (1994) and Gordillo-coworkers (1999). The SRK EOS prediction showed very high deviation of % AARD of 9% - 59%. The Yu model had three derivatized amino acids with average absolute deviation from 2.04, 8.17, 10.96, while the Gordillo model had 0.245, 1.067 and 1.144 for CBZ-valine, CBZ-proline and CBZ-aspartic acid successively. The correlated values had better fit with Gordillo model. The predictive capability and applicability for these amino acid derivatives for both the models demonstrated with correlation coefficient around 0.99 for all the experimental solubility observed.
文摘Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.
文摘The ability of some nanostructured materials to perform as effective heterogeneous catalysts is potentially hindered by the failure of the liquid reactant to effectively wet the solid catalyst surface. In this work, two different chemical reactions, each involving a change of phase from liquid to gas on a solid catalyst surface, are investigated. The first reaction is the catalyzed decomposition of a H<sub>2</sub>O<sub>2</sub> monopropellant within a micro-chemical reactor chamber, decorated with RuO<sub>2</sub> nanorods (NRs). The second reaction involves the electrolysis of dilute aqueous solutions of H<sub>2</sub>SO<sub>4</sub> performed with the cathode electrode coated with different densities and sizes of RuO<sub>2</sub> NRs. In the catalyzed H<sub>2</sub>O<sub>2 </sub>decomposition, the reaction rate is observed to decrease with increasing catalyst surface density because of a failure of the liquid to wet on the catalyst surface. In the electrolysis experiment, however, the reaction rate increased in proportion to the surface density of RuO<sub>2</sub> NRs. In this case, the electrical bias applied to drive the electrolysis reaction also causes an electrostatic force of attraction between the fluid and the NR coated surface, and thus assures effective wetting.
文摘Opaque polyploid cells capable of forming megamitochondria are a constant <span style="font-family:Verdana;">feature in colonies of Ishikawa endometrial epithelia, accounting for ap</span><span style="font-family:Verdana;">proximately 5</span><span style="font-family:Verdana;">% </span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">10% of the cells. Opaque cells appear to communicate with other </span><span style="font-family:Verdana;">opaque cells via membrane extensions and with other cells in a colony by </span><span style="font-family:Verdana;">extra</span></span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">cellular vesicles. Opaque cells form first as rectangular structures, somewhat larger than surrounding monolayer cells. The cells eventually round up, re</span><span style="font-family:Verdana;">maining in the colony for 20 or more hours before detaching. The most </span><span style="font-family:Verdana;">un</span><span style="font-family:Verdana;">usual characteristic of Ishikawa opaque cells is their capacity to form mitonucleons, megamitochondria that surround aggregated chromatin. This</span><span style="font-family:Verdana;"> paper reviews evidence that adaptations resulting in megamitochondria i</span><span style="font-family:Verdana;">nclude a loss of the capacity for oxidative phosphorylation leaving the adapted </span><span style="font-family:Verdana;">megamitochondria reliant on metabolism such as reductive carboxylation.</span></span>
文摘Pediatric autoimmune neuropsychiatric disorders associated with or without streptococcal and other bacterial infections (PANDAS/CANS) are emerging as a featured pediatric disorder. Although there is some controversy regarding treatment approaches, especially related to the behavioral sequelae, we have hypothesized in other published work that it is characterized by the rapid onset of Reward Deficiency Syndrome (RDS) in children. We propose utilizing a multi-systems biological approach involving the coupling of genetic addiction risk testing and pro-dopamine regulation (KB220/POLYGEN®) to help induce “dopamine homeostasis” in patients with PANDAS, especially those with known DNA-induced hypodopaminergia. This case study examines a 12-year-old Caucasian male with no prior psychiatric issues who presented with a sudden onset of severe anxiety, depression, emotional liability, and suicidal ideation. The patient underwent genotyping and the genetic addiction risk score (GARS) testing, which revealed risk polymorphisms in the dopamine D2 (-DRD2/ANKK (Taq1A), OPRM1 (A/G), DRD3 (C/T), and MAOA (4R) genes. These polymorphisms have been linked to hypodopaminergia. The patient was subsequently placed on research ID-KB220ZPBMPOLY (POLYGEN®), and albeit the possibility of bias, based upon self and parental assessment, a marked rapid improvement in psychiatric symptoms was observed. In the second phase of treatment (102 days utilizing KB220), the patient received standard antibody testing, which was positive for Lyme. Antibacterial therapy started immediately, and KB220z was discontinued to provide a wash-out period. A monotonic trend analysis was performed on each outcome measure, and a consistently decreasing trend was observed utilizing antibacterial therapy. Our recommendation, albeit only one case, is to utilize and further research a combined therapeutic approach, involving precision-guided DNA testing and pro-dopamine regulation along with antibacterial therapy, as well as glutathione to address offensive enhanced cytokines, in patients with suspected PANDAS/CANS.
文摘As X-ray computed tomography （CT） is widely used in diagnosis and radiotherapy, it is important to reduce the radiation dose as low as reasonably achievable. For this pur- pose, one may use the TV based methods or wavelet frame based methods to reconstruct high quality images from reduced number of projections. Furthermore, by using the in- terior tomography scheme which only illuminates a region-of-interest （ROI）, one can save more radiation dose. In this paper, a robust wavelet frame regularization based model is proposed for both global reconstruction and interior tomography. The model can help to reduce the errors caused by mismatch of the huge sparse projection matrix. A three-system decomposition scheme is applied to decompose the reconstructed images into three differ- ent parts： cartoon, artifacts and noise. Therefore, by discarding the estimated artifacts and noise parts, the reconstructed images can be obtained with less noise and artifacts. Similar to other frame based image restoration models, the model can be efficiently solved by the split Bregman algorithm. Numerical simulations show that the proposed model outperforms the FBP and SART＋TV methods in terms of preservation of sharp edges, mean structural similarity （SSIM）, contrast-to-noise ratio, relative error and correlation- s. For example, for real sheep lung reconstruction, the proposed method can reach the mean structural similarity as high as 0.75 using only 100 projections while the FBP and the SART^TV methods need more than 200 projections. Additionally, the proposed ro- bust method is applicable for interior and exterior tomography with better performance compared to the FBP and the SART＋TV methods.
