Corn(Zea mays L.)is a very sensitive crop to phosphorus(P)deficiency during the early development phase,which may be a limiting factor for the sustainable production of this crop in P-deficient tropical soils.However,...Corn(Zea mays L.)is a very sensitive crop to phosphorus(P)deficiency during the early development phase,which may be a limiting factor for the sustainable production of this crop in P-deficient tropical soils.However,scientific evidence indicates that inoculation with phosphate-solubilizing bacteria can improve the development,uptake,and P-use efficiency of corn plants.In the present study,two contrasting corn hybrids were investigated for their responsiveness to multiple inoculations of Bacillus subtilis,B.megaterium,B.velezencis,and Pseudomonas fluorescens and application of phosphate fertilizer rates in the sandy soil of the Brazilian Cerrado.Plants from stable(DKB 360 PRO3)and responsive(DKB 255 PRO3)corn hybrids were inoculated with 0 and 2 mL of inoculant containing multiple phosphate-solubilizing bacteria and fertilized with low(0 mg P·kg^(-1)),medium(40 mg P·kg^(-1))and high(80 mg P·kg^(-1))levels of phosphate fertilizer using triple superphosphate(46% of P_(2)O_(5)).Treatments were distributed in a randomized block design using 2×2×3 factorial scheme,with four replicates.Plants were grown in 8-L pots for 70 days under greenhouse conditions.Morphological characteristics,leaf P concentration,and P use efficiency of corn plants were evaluated.Our results showed that the multiple inoculations of Bacillus subtilis,B.megaterium,B.velezencis,and Pseudomonas fluorescens are a promising sustainable agricultural practice to be recommended for corn cultivation,especially because it improves the development and P use efficiency of plants fertilized with medium P levels,which reduces the costs associated with mineral phosphate fertilization,a non-renewable fertilizer source.In sandy tropical soilwith low P availability,applying intermediate rates of highly soluble phosphate fertilizer,such as triple superphosphate,is sufficient to maximize plant development and the nutritional status of corn crops for sustainable production with low environmental impact.展开更多
Nd^(3+)-doped tellurite glasses are promising materials for thermometers based on the fluorescence intensity ratio(FIR)technique.Nevertheless,at high Nd^(3+)concentrations,energy transfer(ET)processes such as optical ...Nd^(3+)-doped tellurite glasses are promising materials for thermometers based on the fluorescence intensity ratio(FIR)technique.Nevertheless,at high Nd^(3+)concentrations,energy transfer(ET)processes such as optical reabsorption and cross-relaxation can affect the Nd^(3+)emission,which has been little explored in the literature.Therefore,the present work investigated the use of Nd^(3+)-doped tellurite glass(samples doped with Nd^(3+)at 0.2 mol%,0.5 mol%,2.0 mol%,and 4.0 mol%)in fluorescence thermometers,in the temperature range from 299 to 371 K.The results indicate a strong dependence of the FIR parameters on the Nd^(3+)concentration,due to changes in the emission band profiles caused by optical reabsorption of the Nd^(3+)emissions and cross-relaxation processes.A decrease of the relative sensitivity of the ratio^(4)F_(5/2)→^(4)I_(9/2)/^(4)F_(3/2)→^(4)I_(9/2)is observed for samples doped with higher amounts of Nd^(3+).The maximum relative sensitivity at 299 K is 3.00%/K,which is the highest value among the reported Nd^(3+)ions.展开更多
Particulate organic matter(POM)is an important energy source for aquatic consumers,understanding its origin and composition is essential for understanding the energetic dynamics of aquatic environments.The aim of this...Particulate organic matter(POM)is an important energy source for aquatic consumers,understanding its origin and composition is essential for understanding the energetic dynamics of aquatic environments.The aim of this study was to analyze the relationship between POM and phytoplankton(isotopic values and chlorophyll concentration)and abiotic variables during dry and rainy seasons.Sampling was conducted in rivers and lagoons in the floodplain of the Upper ParanáRiver.We found a greater difference in ^(δ13)C values of POM between sampling points than between seasons,indicating that the composition of regional sources influences the composition of POM more than dry and rainy seasons.In addition,the concentration of chlorophyll during the dry season was positively correlated with ^(δ13)C values during that rainy period.Additionally,we found a relationship between factors limiting the growth of phytoplankton and ^(δ13)C values of POM,such as phosphate ions,indicating that variables that regulate phytoplankton growth tend to influence the composition of POM in river floodplains.Therefore,maintaining the variables that regulate the phytoplankton community is of fundamental importance for the composition of POM,an important energy source in aquatic environments.展开更多
Background The quality of cotton fiber determines its value in the textile market,influencing agricultural profitability and the efficiency of textile processing.The selection of genotypes with superior fibers is a ke...Background The quality of cotton fiber determines its value in the textile market,influencing agricultural profitability and the efficiency of textile processing.The selection of genotypes with superior fibers is a key factor for genetic improvement programs seeking adaptability and sustainability in the face of climate change.This demonstrates the strategic importance of this plant for sustainable agriculture and the global textile industry.The objective of this research was to decipher the fiber quality of Gossypium barbadense var.brasiliensis in the native Amazonian communities of La Convención,Cusco-Perú,and to evaluate other critical aspects of native cotton that have not yet been identified.The methodology included non-probability sampling for accessibility,qualitative and quantitative analyses,and multivariate analyses.The fiber length(mm),micronaire index(maturity/fineness),fiber strength(gf/tex),length uniformity index(%),fiber elongation(%),maturation index(%),and short fiber index(%)were the fiber characteristics evaluated using the HVI method in cotton genotypes.Results Cotton accessions collected from Koribeni(Gossypium spp.)and Shivankoreni(Gossypium barbadense var.brasiliensis)stood out for their fiber quality properties,especially length,strength,and uniformity,which highlights their relevance for advanced textile applications and potential for use in plant genetic improvement programs.Conclusion These findings reinforce the need to conserve and study these native cotton accessions from the Peruvian Amazon region,which can offer promising perspectives for the textile industry and agricultural biodiversity.展开更多
The study aims to measure virtual water flows in Brazil’s international trade. The methodology is based on the input-output matrix, and the database used was the Eora Global Supply Chain Database. The results showed ...The study aims to measure virtual water flows in Brazil’s international trade. The methodology is based on the input-output matrix, and the database used was the Eora Global Supply Chain Database. The results showed that Brazil exported 230.8 billion m3 of virtual water per year, representing approximately 34.7% of the water footprint of the national production system in 2015. Virtual water imports totaled 111.6 billion m3 with a positive balance (net exports) of 119.2 billion m3. The country is a net exporter of this resource except for trade relations with South America and Africa, regions with negative balances of −36 billion m3 and 3 billion m3, respectively. The main destinations for virtual water exports from Brazil are Europe, with 41% of the total exported, followed by Asia and North America, with values close to 20%. The fact that Brazil is a net exporter of water makes sustainable use of the resource important, as the diversity of climate, soil, and water availability at a regional level is a challenge, which makes it essential to increase the efficiency of the use and management of water resources.展开更多
Polysaccharide-based composite materials(beads) containing silver nanoparticles(AgNPs) were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag* delivery.Beads promoted the AgNPs protection ...Polysaccharide-based composite materials(beads) containing silver nanoparticles(AgNPs) were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag* delivery.Beads promoted the AgNPs protection and inhibited their aggregation.Antimicrobial assays showed that the beads/AgNPs concentration can be modulated to deliver an amount of Ag* necessary for kill Escherichia coli cells.展开更多
According to the main international clinical guidelines,the recommended treatment for locally-advanced rectal cancer is neoadjuvant chemoradiotherapy followed by surgery.However,doubts have been raised about the appro...According to the main international clinical guidelines,the recommended treatment for locally-advanced rectal cancer is neoadjuvant chemoradiotherapy followed by surgery.However,doubts have been raised about the appropriate definition of clinical complete response(cCR)after neoadjuvant therapy and the role of surgery in patients who achieve a cCR.Surgical resection is associated with significant morbidity and decreased quality of life(QoL),which is especially relevant given the favourable prognosis in this patient subset. Accordingly, therehas been a growing interest in alternative approaches with less morbidity,including the organ-preserving watch and wait strategy, in which surgery isomitted in patients who have achieved a cCR. These patients are managed with aspecific follow-up protocol to ensure adequate cancer control, including the earlyidentification of recurrent disease. However, there are several open questionsabout this strategy, including patient selection, the clinical and radiologicalcriteria to accurately determine cCR, the duration of neoadjuvant treatment, therole of dose intensification (chemotherapy and/or radiotherapy), optimal followupprotocols, and the future perspectives of this approach. In the present review,we summarize the available evidence on the watch and wait strategy in thisclinical scenario, including ongoing clinical trials, QoL in these patients, and thecontroversies surrounding this treatment approach.展开更多
Solid-state cooling based on caloric effects is considered a viable alternative to replace the conventional vapor-compression refrigeration systems.Regarding barocaloric materials,recent results show that elastomers a...Solid-state cooling based on caloric effects is considered a viable alternative to replace the conventional vapor-compression refrigeration systems.Regarding barocaloric materials,recent results show that elastomers are promising candidates for cooling applications around room-temperature.In the present paper,we report supergiant barocaloric effects observed in acetoxy silicone rubber—a very popular,low-cost and environmentally friendly elastomer.Huge values of adiabatic temperature change and reversible isothermal entropy change were obtained upon moderate applied pressures and relatively low strains.These huge barocaloric changes are associated both to the polymer chain rearrangements induced by confined compression and to the first-order structural transition.The results are comparable to the best barocaloric materials reported so far,opening encouraging prospects for the application of elastomers in near future solid-state cooling devices.展开更多
Management of discarded tires is a compelling environmental issue worldwide.Although there are several approaches developed to recycle waste tire rubbers,their application in solid-state cooling is still unexplored.Co...Management of discarded tires is a compelling environmental issue worldwide.Although there are several approaches developed to recycle waste tire rubbers,their application in solid-state cooling is still unexplored.Considering the high barocaloric potential verified for elastomers,the use of waste tire rubber(WTR)as a refrigerant in solid-state cooling devices is very promising.Herein,we investigated the barocaloric effects in WTR and polymer blends made of vulcanized natural rubber(VNR)and WTR,to evaluate its feasibility for solid-state cooling technologies.The adiabatic temperature changes and the isothermal entropy changes reach giant values,as well as the performance parameters,being comparable or even better than most barocaloric materials in literature.Moreover,pure WTR and WTR-based samples also present a faster thermal exchange than VNR,consisting of an additional advantage of using these discarded materials.Thus,the present findings evidence the encouraging perspectives of employing waste rubbers in solid-state cooling based on barocaloric effects,contributing to both the recycling of polymers and the sustainable energy technology field.展开更多
In order to obtain with simplicity the known and new properties of linear canonical transformations (LCTs), we show that any relation between a couple of operators (A,B) having commutator identical to unity, called du...In order to obtain with simplicity the known and new properties of linear canonical transformations (LCTs), we show that any relation between a couple of operators (A,B) having commutator identical to unity, called dual couple in this work, is valuable for any other dual couple, so that from the known translation operator exp(a∂<sub>x</sub>) one may obtain the explicit form and properties of a category of linear and linear canonical transformations in 2N-phase spaces. Moreover, other forms of LCTs are also obtained in this work as so as the transforms by them of functions by integrations as so as by derivations. In this way, different kinds of LCTs such as Fast Fourier, Fourier, Laplace, Xin Ma and Rhodes, Baker-Campbell-Haussdorf, Bargman transforms are found again.展开更多
Peach trees are temperate climate fruit trees most planted in subtropical and tropical regions. This advance of the peach crop is mainly due to the introduction of less cold-demanding new cultivar varieties and which ...Peach trees are temperate climate fruit trees most planted in subtropical and tropical regions. This advance of the peach crop is mainly due to the introduction of less cold-demanding new cultivar varieties and which require using less specific techniques, such as plant growth regulators and pruning. Within this context, the objective of this work was to evaluate phenological aspects and the yield potential of the cultivars Granada, Aurora-1, Dourado-2, Douradao, Big-Aurora, Marli and Chiripá, grafted onto the “Okinawa” rootstock, planted in the region of Sao Manuel-SP, during two cultivation cycles. The experimental design used was a completely randomized design with 7 treatments and 5 replicates, with the experimental unit represented by two plants.”Granada” had early harvest in mid-September, and “Chiripá” in late November and early December, in which the former was the early-ripening cultivar and the latter was the late-ripening one. “Aurora-1” and “Dourado-2”, had the highest yield values, 18.95 and 16.57 t·ha-1 respectively, followed by “Big-Aurora” with yield values of 12.13 t·ha-1. For subtropical regions, such as São Manuel-SP, less cold-demanding cultivars are recommended, such as Aurora-1, Dourado-2 and Big-Aurora. The planting of early- and late-ripening varieties, such as Granada and Chiripá, respectively, is an interesting alternative for producers wanting to scale their production.展开更多
Chemical pesticides tend to accumulate in soil, resulting in human and environmental health risks. Hence, alternative methodologies involving chemical pesticides are beneficial for the control of agricultural pests. &...Chemical pesticides tend to accumulate in soil, resulting in human and environmental health risks. Hence, alternative methodologies involving chemical pesticides are beneficial for the control of agricultural pests. </span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">Metarhizium anisopliae</i></span></i> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">is an entomopathogenic fungus that acts on different developmental stages of pest insects such as <i></span><i><span style="font-family:Verdana;">Diatraea saccharalis</i></span></i><span style="font-family:Verdana;">, a holometabolic lepidopteran with high potential for infestation in sugarcane crops. The present study evaluated the biocontrol effect of <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> isolates MT and E9 on <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> eggs at different ages by investigating the external and internal morphological alterations in treated eggs. Conidial suspensions of <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> isolated from MT and E9 at concentrations of 10<sup></span><sup><span style="font-family:Verdana;">7</span></sup><span style="font-family:Verdana;"></sup> conidia/mL were applied to eggs of <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> aged 0, 24, 48, 72, 96 and 120 h. The eggs were observed every 24 h during development (0 h to 144 h). Samples were collected for observational, histological, and ultrastructural analyses. We found that the MT isolate caused 100% inviability of eggs aged 0 - 72 h, 144 h after the bioassays, while the effect of the E9 isolate varied between 49.40% and 93.75%. Melanization was observed on the periphery of the eggs 24 h after the bioassays. Fungal hyphae developed 48 h after bioassays, crossed the egg chorion, and dispersed through the yolk region, inhibiting embryonic development. After 72 h, hyphae and conidiophores were observed on the eggs, which persisted for 144 h. In sum, <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> MT isolate can be used as a biological controller for <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> eggs.展开更多
Tree bark is one of the most important non-timber forest products. In less developed countries, it is used for multiple purposes, particularly in traditional medicine. This paper addresses the question of bark exploit...Tree bark is one of the most important non-timber forest products. In less developed countries, it is used for multiple purposes, particularly in traditional medicine. This paper addresses the question of bark exploitation, uses, and impacts in Madjadjane village, southern Mozambique. For that, we have conducted an ethnobotanical survey and analysed the level of damage of the ten most exploited tree species. Bark was mainly used for medical purposes, spanning 13 different applications. Most of the species had more than one medical application constituting potential sources of valuable biocompounds. In general the level of damage caused by debarking was not critical, but should be seen with caution. An upgrade and update of the results will be of utmost importance to estimate with more accuracy the current conservation status as well as to predict future impacts and define better conservation strategies. We suggest the expansion of ethnobotanical surveys as well as their integration in broad programs aimed at the preservation and valorization of local heritage. This will encourage equitable access and benefit sharing of biodiversity as well as the promotion of bio-based economy.展开更多
In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vei...In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vein in monovascular perfusion (entry: portal vein;exit: hepatic vein). In the present communication we are presenting and discussing experiments done with the bivascularly perfused rat liver (entry: portal vein + hepatic artery;exit: hepatic vein) in order to verify if the hemodynamic effects also occur in the arterial bed. It was found that the A. blazei extract is also active when infused into the hepatic arterial bed, with differences in both sensitivity and nature of the effects on either perfusion pressure or oxygen consumption. Constriction of the arterial bed required much higher concentrations of the extract than the portal bed. The kinetics of the response was also different, with a biphasic instead of a monophasic response. These results provide a promising starting point for future studies aiming to bring to light more mechanistic details about these and possibly other effects.展开更多
This study aimed determine the activity of aqueous and ethanolic extracts of Origanum vulgare against some viruses of veterinary importance (bovine viral diarrhea virus (BVDV), equine arteritis virus (EAV), equine inf...This study aimed determine the activity of aqueous and ethanolic extracts of Origanum vulgare against some viruses of veterinary importance (bovine viral diarrhea virus (BVDV), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV), canine distemper virus (CDV), canine adenovirus (CAV), and canine cororavirus (CCoV) by evaluating the possibility of inhibition of viral particles production. The aqueous extract from 1600 μg/mL did not show cytotoxicity for all cellular lineages evaluated, Madin Darby bovine kidney cells (MDBK), Rabbit kidney cells (RK 13), Madin Darby canine kidney cells (MDCK) and Crandell feline kidney cells (CRFK), and the ethanolic extract of Origanum vulgare was not toxic at 600 μg/mL. The addition of aqueous extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 infecting dose for cellular culture at 50% (TCID50) to 102.09 TCID50/100 μL while in the presence of the ethanolic extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 TCID50 to 100.79 TCID50/100 μL. To CDV the addition of aqueous extract resulted in a reduction from 102.00 TCID50 to 100.00 TCID50/100 μL while in the presence of the ethanolic extract titers were reduced from 102.00 TCID50 to 101.50 TCID50/100 μL. No significant differences in titers regarding the others analyzed viruses were detected. With respect to chemical analysis of the extracts of Origanum vulgare, were identified in the ethanol extract phenolics rosmarinic acid, caffeic acid, carnosol, p-coumaric acid, carnosic acid, luteolin, apigenin, kaempferol and quercetin. In aqueous extracts of Origanum vulgare were detected rosmarinic acid, p-coumaric acid carnosic acid, luteolin, apigenin, kaempferol and quercetin. The data obtained stimulate other biological assays in order to determine which compounds are responsible for the antiviral activity as well as which are the mechanisms involved. The results presented and the considerations we were able to draw from them allowed us to conclude that the ethanolic extract of Origanum vulgare demonstrated lower cell viability than the aqueous extract and has significant antiviral activity against EAV and the both aqueous and ethanolic extracts have antiviral action against CDV.展开更多
In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extending from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum ...In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extending from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum to form the cryptonephridium. Scanning and transmission electron microscopy of non-parasitized and parasitized larvae by Cotesia flavipes have indicated that the tubules consist of secretory and reabsorption cells. In parasitized larvae, the occurrence of hemocytes and teratocytes around the tubules is indicative of their role in immunological defense;however, they were not observed in non-parasitized larvae. At day 9 of parasitism, the mitochondria-containing vacuoles and myelin-like figures show signs of degeneration. The results of this study have confirmed that C. flavipes manipulates the physiology and biochemistry of D. saccharalis because the Malpighian tubules of the parasitized larvae remain active until the parasitoid completes its pupal stage and is released from the host organism.展开更多
<strong>Background:</strong> the physical integration of South America through a Bioceanic Corridor has been idealized for a long time by the countries that make up Mercosur. This integration will have imp...<strong>Background:</strong> the physical integration of South America through a Bioceanic Corridor has been idealized for a long time by the countries that make up Mercosur. This integration will have impacts on the population residing in the cities where the route will be built. Among them, Porto Murtinho, southern Mato Grosso on the border with Paraguay, stands out, where a bridge will be built over the Paraguay River that will link these two countries. <strong>Methods:</strong> a retrospective, quantitative and cross-sectional study of a descriptive type of documentary approach that sought to analyze the prevalence of arboviruses, Sexually Transmitted Infections (STIs) and Diseases Related to Inadequate Environmental Sanitation (DRIES) notified and stored in the National System of Notifiable Diseases (SINAN). The sample consisted of new cases of these pathologies in the municipality of Porto Murtinho registered between January 2017 and December 2020. <strong>Results:</strong> there was an increase of 10% in the total number of diseases within this period. The number of dengue cases increased 36 times;the incidence of Syphilis, HIV and HPV decreased;and, finally, maintenance of cases of Gonorrhea, Leptospirosis, Yellow Fever, Zika, Chikungunya, Hepatitis A and B. <strong>Conclusions:</strong> actions are needed to prevent the increase of these pathologies together with the construction of the Bioceanic Route, thus avoiding damage population health and increased consumption of government resources.展开更多
The aim of the present study was to present the wealth, ecological characteristics and the floristic similarity of ferns and lycophytes from two forest areas of the municipality of Campo Mour?o, PR, Brazil. The vegeta...The aim of the present study was to present the wealth, ecological characteristics and the floristic similarity of ferns and lycophytes from two forest areas of the municipality of Campo Mour?o, PR, Brazil. The vegetation of the municipality is characterized mainly by an ecotone between the Seasonal Semideciduous Forest and Mixed Ombrophilous Forest. We recorded 56 species, distributed in 31 genera and 16 families. The most representative families were Pteridaceae (14) and Polypodiaceae (11) and the wealthier genre was Thelypteris (6). The terricolous species were predominant (72%) and the preferential environments were riparian vegetation and forest interior (70%). The flora of Campo Mour?o was more similar to the studies conducted in the state of Rio Grande do Sul, and the cophenetic correlation coefficient (r = 0.9058) showed a consistent adjust. In the principal components analysis (PCA) the variance explained by the two principal components was 72.99%. The wealth found in this study corresponds to approximately 11.4% of the flora of ferns and lycophytes of Paraná. Future contributions are needed to increase the knowledge about the flora of ferns and lycophytes in areas of ecotone, mainly in the region of Campo Mour?o, where the studies are scarce.展开更多
The number of treatment options for metastatic hormone-sensitive prostate cancer has increased substantially in recent years.The classic treatment approach for these patients—androgen-deprivation therapy alone—is no...The number of treatment options for metastatic hormone-sensitive prostate cancer has increased substantially in recent years.The classic treatment approach for these patients—androgen-deprivation therapy alone—is now considered suboptimal.Several randomized phase III clinical trials have demonstrated significant clinical benefits—including significantly better overall survival and quality of life—for treatments that combine androgen-deprivation therapy with docetaxel,abiraterone acetate,enzalutamide,apalutamide,and/or radiotherapy to the primary tumour.As a result,these approaches are now included in treatment guidelines and considered standard of care.However,the different treatment strategies have not been directly compared,and thus treatment selection remains at the discretion of the individual physician or,ideally,a multidisciplinary team.Given the range of available treatment approaches with varying toxicity profiles,treatment selection should be individualized based on the patient’s clinical characteristics and preferences,which implies active patient participation in the decision-making process.In the present document,we discuss the changing landscape of the management of patients with metastatic hormonesensitive prostate cancer in the context of several recently-published landmark randomized trials.In addition,we discuss several unresolved issues,including the optimal sequencing of systemic treatments and the incorporation of local treatment of the primary tumour and metastases.展开更多
This research assessed the relationship between wood physical-anatomical variables and charcoal physical-mechanical properties.Nine trees were harvested and mechanically processed into standard pieces for laboratory a...This research assessed the relationship between wood physical-anatomical variables and charcoal physical-mechanical properties.Nine trees were harvested and mechanically processed into standard pieces for laboratory analysis.The following variables were determined:wood density,fiber length and width,fiber lumen diameter and wall thickness,fiber wall fraction,and vessel frequency.The charcoal was produced in a laboratory and gravimetric yield,relative density,and mechanical properties established.Pearson’s correlation coefficients,multivariate statistical analysis of canonical correlation,and principal component analysis were used to evaluate the relationship between wood and charcoal properties.Fiber width was not significantly correlated with charcoal properties.Charcoal bulk density,hardness,and strength were positively correlated with wood density,fiber length,wall thickness,and wall fraction.Charcoal properties were negatively correlated with fiber lumen diameter and vessel frequency.These results will help identify the best wood with adequate physical-mechanical properties for industrial charcoal proces sing.展开更多
基金financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nível Superior-Brasil(CAPES)-Finance Code 001financial resources from the Fundacao de Apoio ao Desenvolvimento do Ensino,Ciencia e Tecnologia do Estado de Mato Grosso do Sul—FUNDECT(Termo de Outorga:133/2023/SIAFEM:33108).
文摘Corn(Zea mays L.)is a very sensitive crop to phosphorus(P)deficiency during the early development phase,which may be a limiting factor for the sustainable production of this crop in P-deficient tropical soils.However,scientific evidence indicates that inoculation with phosphate-solubilizing bacteria can improve the development,uptake,and P-use efficiency of corn plants.In the present study,two contrasting corn hybrids were investigated for their responsiveness to multiple inoculations of Bacillus subtilis,B.megaterium,B.velezencis,and Pseudomonas fluorescens and application of phosphate fertilizer rates in the sandy soil of the Brazilian Cerrado.Plants from stable(DKB 360 PRO3)and responsive(DKB 255 PRO3)corn hybrids were inoculated with 0 and 2 mL of inoculant containing multiple phosphate-solubilizing bacteria and fertilized with low(0 mg P·kg^(-1)),medium(40 mg P·kg^(-1))and high(80 mg P·kg^(-1))levels of phosphate fertilizer using triple superphosphate(46% of P_(2)O_(5)).Treatments were distributed in a randomized block design using 2×2×3 factorial scheme,with four replicates.Plants were grown in 8-L pots for 70 days under greenhouse conditions.Morphological characteristics,leaf P concentration,and P use efficiency of corn plants were evaluated.Our results showed that the multiple inoculations of Bacillus subtilis,B.megaterium,B.velezencis,and Pseudomonas fluorescens are a promising sustainable agricultural practice to be recommended for corn cultivation,especially because it improves the development and P use efficiency of plants fertilized with medium P levels,which reduces the costs associated with mineral phosphate fertilization,a non-renewable fertilizer source.In sandy tropical soilwith low P availability,applying intermediate rates of highly soluble phosphate fertilizer,such as triple superphosphate,is sufficient to maximize plant development and the nutritional status of corn crops for sustainable production with low environmental impact.
基金Project supported by National Council for Scientific and Technological Development(CNPq)(#305067/2019-2,#303707/2022-4,#306452/2018-9)the Development of Education and Science and Technology of the State of Mato Grosso do Sul(FUNDECT)(#59/300.634/2016,#71/027.247/2022)。
文摘Nd^(3+)-doped tellurite glasses are promising materials for thermometers based on the fluorescence intensity ratio(FIR)technique.Nevertheless,at high Nd^(3+)concentrations,energy transfer(ET)processes such as optical reabsorption and cross-relaxation can affect the Nd^(3+)emission,which has been little explored in the literature.Therefore,the present work investigated the use of Nd^(3+)-doped tellurite glass(samples doped with Nd^(3+)at 0.2 mol%,0.5 mol%,2.0 mol%,and 4.0 mol%)in fluorescence thermometers,in the temperature range from 299 to 371 K.The results indicate a strong dependence of the FIR parameters on the Nd^(3+)concentration,due to changes in the emission band profiles caused by optical reabsorption of the Nd^(3+)emissions and cross-relaxation processes.A decrease of the relative sensitivity of the ratio^(4)F_(5/2)→^(4)I_(9/2)/^(4)F_(3/2)→^(4)I_(9/2)is observed for samples doped with higher amounts of Nd^(3+).The maximum relative sensitivity at 299 K is 3.00%/K,which is the highest value among the reported Nd^(3+)ions.
基金Supported by the Research Nucleus in LimnologyIchthyology and Aquaculture (NUPELIA) for logistic support+4 种基金the Laboratory of Energetic Ecology and the Long-term Ecological Research Program (PELD/CNPq)Site 6-PIAP (upper ParanáRiver floodplain)PROEXUEMand Fundação Araucária for the scholarship
文摘Particulate organic matter(POM)is an important energy source for aquatic consumers,understanding its origin and composition is essential for understanding the energetic dynamics of aquatic environments.The aim of this study was to analyze the relationship between POM and phytoplankton(isotopic values and chlorophyll concentration)and abiotic variables during dry and rainy seasons.Sampling was conducted in rivers and lagoons in the floodplain of the Upper ParanáRiver.We found a greater difference in ^(δ13)C values of POM between sampling points than between seasons,indicating that the composition of regional sources influences the composition of POM more than dry and rainy seasons.In addition,the concentration of chlorophyll during the dry season was positively correlated with ^(δ13)C values during that rainy period.Additionally,we found a relationship between factors limiting the growth of phytoplankton and ^(δ13)C values of POM,such as phosphate ions,indicating that variables that regulate phytoplankton growth tend to influence the composition of POM in river floodplains.Therefore,maintaining the variables that regulate the phytoplankton community is of fundamental importance for the composition of POM,an important energy source in aquatic environments.
基金funded by Universidad Nacional Intercultural de Quillabamba,grant number 031-2023-CCO-UNIQ in the project“Variabilidad genética,distribución,impacto socioeconómico y calidad de algodónGossypiumspp.en Echarate y Megantoni Provincia de La Convención-Cusco”。
文摘Background The quality of cotton fiber determines its value in the textile market,influencing agricultural profitability and the efficiency of textile processing.The selection of genotypes with superior fibers is a key factor for genetic improvement programs seeking adaptability and sustainability in the face of climate change.This demonstrates the strategic importance of this plant for sustainable agriculture and the global textile industry.The objective of this research was to decipher the fiber quality of Gossypium barbadense var.brasiliensis in the native Amazonian communities of La Convención,Cusco-Perú,and to evaluate other critical aspects of native cotton that have not yet been identified.The methodology included non-probability sampling for accessibility,qualitative and quantitative analyses,and multivariate analyses.The fiber length(mm),micronaire index(maturity/fineness),fiber strength(gf/tex),length uniformity index(%),fiber elongation(%),maturation index(%),and short fiber index(%)were the fiber characteristics evaluated using the HVI method in cotton genotypes.Results Cotton accessions collected from Koribeni(Gossypium spp.)and Shivankoreni(Gossypium barbadense var.brasiliensis)stood out for their fiber quality properties,especially length,strength,and uniformity,which highlights their relevance for advanced textile applications and potential for use in plant genetic improvement programs.Conclusion These findings reinforce the need to conserve and study these native cotton accessions from the Peruvian Amazon region,which can offer promising perspectives for the textile industry and agricultural biodiversity.
文摘The study aims to measure virtual water flows in Brazil’s international trade. The methodology is based on the input-output matrix, and the database used was the Eora Global Supply Chain Database. The results showed that Brazil exported 230.8 billion m3 of virtual water per year, representing approximately 34.7% of the water footprint of the national production system in 2015. Virtual water imports totaled 111.6 billion m3 with a positive balance (net exports) of 119.2 billion m3. The country is a net exporter of this resource except for trade relations with South America and Africa, regions with negative balances of −36 billion m3 and 3 billion m3, respectively. The main destinations for virtual water exports from Brazil are Europe, with 41% of the total exported, followed by Asia and North America, with values close to 20%. The fact that Brazil is a net exporter of water makes sustainable use of the resource important, as the diversity of climate, soil, and water availability at a regional level is a challenge, which makes it essential to increase the efficiency of the use and management of water resources.
基金the financial support (Nos.481424/2010-5 and 308337/2013-1)
文摘Polysaccharide-based composite materials(beads) containing silver nanoparticles(AgNPs) were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag* delivery.Beads promoted the AgNPs protection and inhibited their aggregation.Antimicrobial assays showed that the beads/AgNPs concentration can be modulated to deliver an amount of Ag* necessary for kill Escherichia coli cells.
文摘According to the main international clinical guidelines,the recommended treatment for locally-advanced rectal cancer is neoadjuvant chemoradiotherapy followed by surgery.However,doubts have been raised about the appropriate definition of clinical complete response(cCR)after neoadjuvant therapy and the role of surgery in patients who achieve a cCR.Surgical resection is associated with significant morbidity and decreased quality of life(QoL),which is especially relevant given the favourable prognosis in this patient subset. Accordingly, therehas been a growing interest in alternative approaches with less morbidity,including the organ-preserving watch and wait strategy, in which surgery isomitted in patients who have achieved a cCR. These patients are managed with aspecific follow-up protocol to ensure adequate cancer control, including the earlyidentification of recurrent disease. However, there are several open questionsabout this strategy, including patient selection, the clinical and radiologicalcriteria to accurately determine cCR, the duration of neoadjuvant treatment, therole of dose intensification (chemotherapy and/or radiotherapy), optimal followupprotocols, and the future perspectives of this approach. In the present review,we summarize the available evidence on the watch and wait strategy in thisclinical scenario, including ongoing clinical trials, QoL in these patients, and thecontroversies surrounding this treatment approach.
文摘Solid-state cooling based on caloric effects is considered a viable alternative to replace the conventional vapor-compression refrigeration systems.Regarding barocaloric materials,recent results show that elastomers are promising candidates for cooling applications around room-temperature.In the present paper,we report supergiant barocaloric effects observed in acetoxy silicone rubber—a very popular,low-cost and environmentally friendly elastomer.Huge values of adiabatic temperature change and reversible isothermal entropy change were obtained upon moderate applied pressures and relatively low strains.These huge barocaloric changes are associated both to the polymer chain rearrangements induced by confined compression and to the first-order structural transition.The results are comparable to the best barocaloric materials reported so far,opening encouraging prospects for the application of elastomers in near future solid-state cooling devices.
基金The authors acknowledge financial support from FAPESP(No.2012/03480-0),CNPq and CAPES.The authors also thank LNLS and CNPEM.
文摘Management of discarded tires is a compelling environmental issue worldwide.Although there are several approaches developed to recycle waste tire rubbers,their application in solid-state cooling is still unexplored.Considering the high barocaloric potential verified for elastomers,the use of waste tire rubber(WTR)as a refrigerant in solid-state cooling devices is very promising.Herein,we investigated the barocaloric effects in WTR and polymer blends made of vulcanized natural rubber(VNR)and WTR,to evaluate its feasibility for solid-state cooling technologies.The adiabatic temperature changes and the isothermal entropy changes reach giant values,as well as the performance parameters,being comparable or even better than most barocaloric materials in literature.Moreover,pure WTR and WTR-based samples also present a faster thermal exchange than VNR,consisting of an additional advantage of using these discarded materials.Thus,the present findings evidence the encouraging perspectives of employing waste rubbers in solid-state cooling based on barocaloric effects,contributing to both the recycling of polymers and the sustainable energy technology field.
文摘In order to obtain with simplicity the known and new properties of linear canonical transformations (LCTs), we show that any relation between a couple of operators (A,B) having commutator identical to unity, called dual couple in this work, is valuable for any other dual couple, so that from the known translation operator exp(a∂<sub>x</sub>) one may obtain the explicit form and properties of a category of linear and linear canonical transformations in 2N-phase spaces. Moreover, other forms of LCTs are also obtained in this work as so as the transforms by them of functions by integrations as so as by derivations. In this way, different kinds of LCTs such as Fast Fourier, Fourier, Laplace, Xin Ma and Rhodes, Baker-Campbell-Haussdorf, Bargman transforms are found again.
文摘Peach trees are temperate climate fruit trees most planted in subtropical and tropical regions. This advance of the peach crop is mainly due to the introduction of less cold-demanding new cultivar varieties and which require using less specific techniques, such as plant growth regulators and pruning. Within this context, the objective of this work was to evaluate phenological aspects and the yield potential of the cultivars Granada, Aurora-1, Dourado-2, Douradao, Big-Aurora, Marli and Chiripá, grafted onto the “Okinawa” rootstock, planted in the region of Sao Manuel-SP, during two cultivation cycles. The experimental design used was a completely randomized design with 7 treatments and 5 replicates, with the experimental unit represented by two plants.”Granada” had early harvest in mid-September, and “Chiripá” in late November and early December, in which the former was the early-ripening cultivar and the latter was the late-ripening one. “Aurora-1” and “Dourado-2”, had the highest yield values, 18.95 and 16.57 t·ha-1 respectively, followed by “Big-Aurora” with yield values of 12.13 t·ha-1. For subtropical regions, such as São Manuel-SP, less cold-demanding cultivars are recommended, such as Aurora-1, Dourado-2 and Big-Aurora. The planting of early- and late-ripening varieties, such as Granada and Chiripá, respectively, is an interesting alternative for producers wanting to scale their production.
文摘Chemical pesticides tend to accumulate in soil, resulting in human and environmental health risks. Hence, alternative methodologies involving chemical pesticides are beneficial for the control of agricultural pests. </span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">Metarhizium anisopliae</i></span></i> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">is an entomopathogenic fungus that acts on different developmental stages of pest insects such as <i></span><i><span style="font-family:Verdana;">Diatraea saccharalis</i></span></i><span style="font-family:Verdana;">, a holometabolic lepidopteran with high potential for infestation in sugarcane crops. The present study evaluated the biocontrol effect of <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> isolates MT and E9 on <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> eggs at different ages by investigating the external and internal morphological alterations in treated eggs. Conidial suspensions of <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> isolated from MT and E9 at concentrations of 10<sup></span><sup><span style="font-family:Verdana;">7</span></sup><span style="font-family:Verdana;"></sup> conidia/mL were applied to eggs of <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> aged 0, 24, 48, 72, 96 and 120 h. The eggs were observed every 24 h during development (0 h to 144 h). Samples were collected for observational, histological, and ultrastructural analyses. We found that the MT isolate caused 100% inviability of eggs aged 0 - 72 h, 144 h after the bioassays, while the effect of the E9 isolate varied between 49.40% and 93.75%. Melanization was observed on the periphery of the eggs 24 h after the bioassays. Fungal hyphae developed 48 h after bioassays, crossed the egg chorion, and dispersed through the yolk region, inhibiting embryonic development. After 72 h, hyphae and conidiophores were observed on the eggs, which persisted for 144 h. In sum, <i></span><i><span style="font-family:Verdana;">M. anisopliae</i></span></i><span style="font-family:Verdana;"> MT isolate can be used as a biological controller for <i></span><i><span style="font-family:Verdana;">D. saccharalis</i></span></i><span style="font-family:Verdana;"> eggs.
文摘Tree bark is one of the most important non-timber forest products. In less developed countries, it is used for multiple purposes, particularly in traditional medicine. This paper addresses the question of bark exploitation, uses, and impacts in Madjadjane village, southern Mozambique. For that, we have conducted an ethnobotanical survey and analysed the level of damage of the ten most exploited tree species. Bark was mainly used for medical purposes, spanning 13 different applications. Most of the species had more than one medical application constituting potential sources of valuable biocompounds. In general the level of damage caused by debarking was not critical, but should be seen with caution. An upgrade and update of the results will be of utmost importance to estimate with more accuracy the current conservation status as well as to predict future impacts and define better conservation strategies. We suggest the expansion of ethnobotanical surveys as well as their integration in broad programs aimed at the preservation and valorization of local heritage. This will encourage equitable access and benefit sharing of biodiversity as well as the promotion of bio-based economy.
文摘In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vein in monovascular perfusion (entry: portal vein;exit: hepatic vein). In the present communication we are presenting and discussing experiments done with the bivascularly perfused rat liver (entry: portal vein + hepatic artery;exit: hepatic vein) in order to verify if the hemodynamic effects also occur in the arterial bed. It was found that the A. blazei extract is also active when infused into the hepatic arterial bed, with differences in both sensitivity and nature of the effects on either perfusion pressure or oxygen consumption. Constriction of the arterial bed required much higher concentrations of the extract than the portal bed. The kinetics of the response was also different, with a biphasic instead of a monophasic response. These results provide a promising starting point for future studies aiming to bring to light more mechanistic details about these and possibly other effects.
文摘This study aimed determine the activity of aqueous and ethanolic extracts of Origanum vulgare against some viruses of veterinary importance (bovine viral diarrhea virus (BVDV), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV), canine distemper virus (CDV), canine adenovirus (CAV), and canine cororavirus (CCoV) by evaluating the possibility of inhibition of viral particles production. The aqueous extract from 1600 μg/mL did not show cytotoxicity for all cellular lineages evaluated, Madin Darby bovine kidney cells (MDBK), Rabbit kidney cells (RK 13), Madin Darby canine kidney cells (MDCK) and Crandell feline kidney cells (CRFK), and the ethanolic extract of Origanum vulgare was not toxic at 600 μg/mL. The addition of aqueous extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 infecting dose for cellular culture at 50% (TCID50) to 102.09 TCID50/100 μL while in the presence of the ethanolic extract of Origanum vulgare in media resulted in a significant reduction of the EAV titer from 105.42 TCID50 to 100.79 TCID50/100 μL. To CDV the addition of aqueous extract resulted in a reduction from 102.00 TCID50 to 100.00 TCID50/100 μL while in the presence of the ethanolic extract titers were reduced from 102.00 TCID50 to 101.50 TCID50/100 μL. No significant differences in titers regarding the others analyzed viruses were detected. With respect to chemical analysis of the extracts of Origanum vulgare, were identified in the ethanol extract phenolics rosmarinic acid, caffeic acid, carnosol, p-coumaric acid, carnosic acid, luteolin, apigenin, kaempferol and quercetin. In aqueous extracts of Origanum vulgare were detected rosmarinic acid, p-coumaric acid carnosic acid, luteolin, apigenin, kaempferol and quercetin. The data obtained stimulate other biological assays in order to determine which compounds are responsible for the antiviral activity as well as which are the mechanisms involved. The results presented and the considerations we were able to draw from them allowed us to conclude that the ethanolic extract of Origanum vulgare demonstrated lower cell viability than the aqueous extract and has significant antiviral activity against EAV and the both aqueous and ethanolic extracts have antiviral action against CDV.
文摘In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extending from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum to form the cryptonephridium. Scanning and transmission electron microscopy of non-parasitized and parasitized larvae by Cotesia flavipes have indicated that the tubules consist of secretory and reabsorption cells. In parasitized larvae, the occurrence of hemocytes and teratocytes around the tubules is indicative of their role in immunological defense;however, they were not observed in non-parasitized larvae. At day 9 of parasitism, the mitochondria-containing vacuoles and myelin-like figures show signs of degeneration. The results of this study have confirmed that C. flavipes manipulates the physiology and biochemistry of D. saccharalis because the Malpighian tubules of the parasitized larvae remain active until the parasitoid completes its pupal stage and is released from the host organism.
文摘<strong>Background:</strong> the physical integration of South America through a Bioceanic Corridor has been idealized for a long time by the countries that make up Mercosur. This integration will have impacts on the population residing in the cities where the route will be built. Among them, Porto Murtinho, southern Mato Grosso on the border with Paraguay, stands out, where a bridge will be built over the Paraguay River that will link these two countries. <strong>Methods:</strong> a retrospective, quantitative and cross-sectional study of a descriptive type of documentary approach that sought to analyze the prevalence of arboviruses, Sexually Transmitted Infections (STIs) and Diseases Related to Inadequate Environmental Sanitation (DRIES) notified and stored in the National System of Notifiable Diseases (SINAN). The sample consisted of new cases of these pathologies in the municipality of Porto Murtinho registered between January 2017 and December 2020. <strong>Results:</strong> there was an increase of 10% in the total number of diseases within this period. The number of dengue cases increased 36 times;the incidence of Syphilis, HIV and HPV decreased;and, finally, maintenance of cases of Gonorrhea, Leptospirosis, Yellow Fever, Zika, Chikungunya, Hepatitis A and B. <strong>Conclusions:</strong> actions are needed to prevent the increase of these pathologies together with the construction of the Bioceanic Route, thus avoiding damage population health and increased consumption of government resources.
文摘The aim of the present study was to present the wealth, ecological characteristics and the floristic similarity of ferns and lycophytes from two forest areas of the municipality of Campo Mour?o, PR, Brazil. The vegetation of the municipality is characterized mainly by an ecotone between the Seasonal Semideciduous Forest and Mixed Ombrophilous Forest. We recorded 56 species, distributed in 31 genera and 16 families. The most representative families were Pteridaceae (14) and Polypodiaceae (11) and the wealthier genre was Thelypteris (6). The terricolous species were predominant (72%) and the preferential environments were riparian vegetation and forest interior (70%). The flora of Campo Mour?o was more similar to the studies conducted in the state of Rio Grande do Sul, and the cophenetic correlation coefficient (r = 0.9058) showed a consistent adjust. In the principal components analysis (PCA) the variance explained by the two principal components was 72.99%. The wealth found in this study corresponds to approximately 11.4% of the flora of ferns and lycophytes of Paraná. Future contributions are needed to increase the knowledge about the flora of ferns and lycophytes in areas of ecotone, mainly in the region of Campo Mour?o, where the studies are scarce.
文摘The number of treatment options for metastatic hormone-sensitive prostate cancer has increased substantially in recent years.The classic treatment approach for these patients—androgen-deprivation therapy alone—is now considered suboptimal.Several randomized phase III clinical trials have demonstrated significant clinical benefits—including significantly better overall survival and quality of life—for treatments that combine androgen-deprivation therapy with docetaxel,abiraterone acetate,enzalutamide,apalutamide,and/or radiotherapy to the primary tumour.As a result,these approaches are now included in treatment guidelines and considered standard of care.However,the different treatment strategies have not been directly compared,and thus treatment selection remains at the discretion of the individual physician or,ideally,a multidisciplinary team.Given the range of available treatment approaches with varying toxicity profiles,treatment selection should be individualized based on the patient’s clinical characteristics and preferences,which implies active patient participation in the decision-making process.In the present document,we discuss the changing landscape of the management of patients with metastatic hormonesensitive prostate cancer in the context of several recently-published landmark randomized trials.In addition,we discuss several unresolved issues,including the optimal sequencing of systemic treatments and the incorporation of local treatment of the primary tumour and metastases.
基金the Wood Science and Technology Laboratory of the Federal University of Lavras,Brazil for supporting this research。
文摘This research assessed the relationship between wood physical-anatomical variables and charcoal physical-mechanical properties.Nine trees were harvested and mechanically processed into standard pieces for laboratory analysis.The following variables were determined:wood density,fiber length and width,fiber lumen diameter and wall thickness,fiber wall fraction,and vessel frequency.The charcoal was produced in a laboratory and gravimetric yield,relative density,and mechanical properties established.Pearson’s correlation coefficients,multivariate statistical analysis of canonical correlation,and principal component analysis were used to evaluate the relationship between wood and charcoal properties.Fiber width was not significantly correlated with charcoal properties.Charcoal bulk density,hardness,and strength were positively correlated with wood density,fiber length,wall thickness,and wall fraction.Charcoal properties were negatively correlated with fiber lumen diameter and vessel frequency.These results will help identify the best wood with adequate physical-mechanical properties for industrial charcoal proces sing.