The study was carried out in northTunisia(36.5?N, 10.2?E) in order to get a comprehensive view of the growth dynamic of young olive orchards (Olea europaea L.,). The experiment involved irrigated trees of cultivars Ch...The study was carried out in northTunisia(36.5?N, 10.2?E) in order to get a comprehensive view of the growth dynamic of young olive orchards (Olea europaea L.,). The experiment involved irrigated trees of cultivars Chétoui, Manzanille, Meski and Picholine, planted at 6 ×6 m2spacing. Tree height, shoot length and canopy, fruit and trunk diameters were monitored regularly after plantation on 12 trees per variety. Root development was analyzed on Chétoui trees, only. Growth patterns were established annually for each variety before proposing an average model for each growth parameter. Results showed that tree height, shoot length and trunk diameter grew following an S-shaped curve with maximum annual increases occurring on the 4th year for tree height and a year later for canopy. The minimum gain coincided with the highest fruit load year, indicating that competition for assimilates concerns also young trees. Average growth patterns for tree height and shoots showed sustained rates all over the growing season with seven distinct periods of growth. Rapid growth occurred in April, July, and September, with similar growth trends observed for productive and less productive cultivars. However, the studied varieties behaved differently. Picholine cv., provided the most important increases and was the best water user. Results also showed that most roots were confined to the top soil layers and developed nearby the trunks. High root densities and important water depletion were observed in this area and thus, water and fertilizers should be supplied for young trees at these depths and distances from trunks. Root and canopy development were highly correlated (r = 0.94) and interfered with fruit growth. When trees set their first productions, the root-canopy ratio approximated the unit. An optimum ratio between root length and leaf area was found (2.3 km·m?2) for the 6-year-old tree, indicating good equilibrium between the above and the underground parts. On the basis of these results, a mathematical model was developed allowing a precise estimation of water requirements of olive trees during a period, where ground cover rarely exceeds 30%. We can conclude that all these models, graphic and mathematic give precise information on the occurrence of the various phenophases of young olive trees and may be used for a quantitative appraisal of the performance of olive varieties under a given environment. However, some aspects would be probed deeper and particularly the influence of climatic data on growth dynamic.展开更多
In the present article it will be critically questioned the traditional entrepreneurship education approaches based on a narrow conception of competency, and their values. Assuming the perspective that to be an entrep...In the present article it will be critically questioned the traditional entrepreneurship education approaches based on a narrow conception of competency, and their values. Assuming the perspective that to be an entrepreneur is basically an attitude towards life and the world, there proposed holistic, constructivist and experiential processes and strategies for entrepreneurship education. The ＂entrepreneur XXI＂, must be able to undertake a social function of change, so, an economical and social development more human, ethical and intelligent. Under this assumption, the ＂Tree Model for the Development of Entrepreneurial Competencies＂, that will be discussed globally in the second part of this article, suggests a dynamic and experiential approach ofentrepreneurship education based on the qualification of people＇s behaviour, self-esteem, competencies and experiences; a profile of key behavioural and performance competencies （root）, experimental pedagogical procedures （trunk） and real results within group projects （fruits）. This model has been developed during the last decade （2001-2011）, using a multidisciplinary research-action procedure, within business, education （at different teaching levels） and social project environments.展开更多
Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydr...Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydrate and protein synthesis,enzyme activation,and reactive oxygen species(ROS)metabolism.Magnesium deficiency in fruit trees will directly influence the growth and development of trees and the improvement of fruit quality.This study mainly reviews the nutritional and physiological mechanism of magnesium in plants and the nutritional diagnosis of magnesium deficiency in fruit trees,in order to provide a theoretical basis for further research on magnesium nutrition in fruit trees,and accordingly provide a certain reference for the application of magnesium fertilizer.展开更多
This paper analyzes the current situation of training young scientific and technological talents,attracting talents and making good use of talents in Changli Fruit Tree Research Institute of Hebei Academy of Agricultu...This paper analyzes the current situation of training young scientific and technological talents,attracting talents and making good use of talents in Changli Fruit Tree Research Institute of Hebei Academy of Agricultural and Forestry Sciences.There are some problems,such as lack of talent recruitment planning,and lack of follow-up training mechanism.This paper puts forward some measures,such as formulating a scientific recruitment plan,making a follow-up plan for talent training,building an ability-and performance-oriented evaluation mechanism for scientific and technological talents,so as to attach importance to the training of young scientific research talents and promote the echelon construction of the research institute.展开更多
From two aspects:the composition of scientific research management institutions and the tasks undertaken by scientific research management departments,the present situation of scientific and technological management t...From two aspects:the composition of scientific research management institutions and the tasks undertaken by scientific research management departments,the present situation of scientific and technological management team in agricultural research institutes is expounded.Some problems existed in the scientific and technological management team of provincial agricultural research institutes are analyzed,such as uneven knowledge structure of scientific research management personnel,low degree of specialization,poor stability,lack of professional and systematic job training,etc.Professional development countermeasures are proposed,such as optimizing the structure of scientific research management team,establishing a professional management system with the characteristics of agricultural scientific research institutions,formulating corresponding evaluation mechanism for scientific research management personnel,and strengthening the training of agricultural scientific research management personnel.展开更多
Current situation of introduction,cultivation and use of high-level talents in agricultural research institutes are analyzed,and the optimized development strategy of further strengthening the construction,introductio...Current situation of introduction,cultivation and use of high-level talents in agricultural research institutes are analyzed,and the optimized development strategy of further strengthening the construction,introduction,cultivation and use of high-level talent team is thought.It plays a positive role in provincial agricultural research institutes discovering,cultivating and gathering a number of high-quality talents and high-level innovation teams.展开更多
Based on the recently published whole-genome sequence of cultivated strawberry ’Camarosa’, in this study, 222FaWRKY genes were identified in the ’Camarosa’ genome. Phylogenetic analysis showed that the 222 FaWRKY ...Based on the recently published whole-genome sequence of cultivated strawberry ’Camarosa’, in this study, 222FaWRKY genes were identified in the ’Camarosa’ genome. Phylogenetic analysis showed that the 222 FaWRKY candidate genes were classified into three groups, of which 41 were in group Ⅰ, 142 were in group Ⅱ, and 39 were in group Ⅲ. The 222 FaWRKY genes were evenly distributed among the seven chromosomes. The exon–intron structures and motifs of the WRKY genes had evolutionary diversity in different cultivated strawberry genomes. Regarding differential expression, the expression of FaWRKY133 was relatively high in leaves, while FaWRKY63 was specifically expressed in roots. FaWRKY207, 59, 46, 182, 156, 58, 39, 62 and 115 were up-regulated during achene development from the green to red fruit transition. FaWRK181, 166 and 211 were highly expressed in receptacles at the ripe fruit stage. One interesting finding was that Fa WRKY179 and 205 were significantly repressed after Colletotrichum fructicola inoculation in both ’Benihoppe’ and ’Sweet Charlie’ compared with Mock. The data reported here provide a foundation for further comparative genomics and analyses of the distinct expression patterns of FaWRKY genes in various tissues and in response to C. fructicola inoculation.展开更多
Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the females...Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the femalespecific marker MrFT2_BD-SEX.The integrated map included eight linkage groups and spanned 491 cM.Eleven sexassociated markers were identified,six of which were located in linkage group 8,in agreement with the previously reported location of the sex-determining region.The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions.Among the females of the accessions,we found two female-specific alleles,designated W-b(151 bp)and W-d(129 bp).We previously found that‘Dongkui’,a female cultivar,could produce viable pollen(we refer to such plants‘Dongkui-male’)and serve as the paternal parent in crosses.The genotypes of the MrFT2_BD-SEX marker were W-b/Z in‘Biqi’and W-d/Z in‘Dongkui-male’.The progeny of a cross between these parents produced a 3:1 female(W-)to male(ZZ)ratio and the expected 1:1:1:1 ratio of W-b/W-d:W-b/Z:W-d/Z:Z/Z.In addition,the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes.Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus(W)in a small genomic region(59 kb and less than 3.3 cM).Furthermore,we have produced a homozygous“super female”(WW)that should produce all-female offspring in the F2 generation,providing a foundation for commercial use and presenting great potential for use in modern breeding programs.展开更多
In order to explore the extraction method of carotenoids from citrus leaves,the extraction effect of carotenoids from leaves of Honey pomelo by ultrasonic-assisted extraction was investigated by single factor experime...In order to explore the extraction method of carotenoids from citrus leaves,the extraction effect of carotenoids from leaves of Honey pomelo by ultrasonic-assisted extraction was investigated by single factor experiment and orthogonal experiment.The results showed that the optimum extraction condition was:the ration of ethanol and acetone 1∶1(v/v,containing 0.1%BHT),solid-liquid ratio 1∶30(g/mL),extraction time 7 min,ultrasonic power 240 W,extraction frequency 3 times.Under the above condition,the extraction rate of carotenoids was 85.98%.展开更多
This study investigated the direct effect of the insecticides deltamethrin and spinosad on three egg parasitoids species: Trichogramma oleae, T. cacoeciae and T. bourarachae. The parasitoid pupae were exposed to pesti...This study investigated the direct effect of the insecticides deltamethrin and spinosad on three egg parasitoids species: Trichogramma oleae, T. cacoeciae and T. bourarachae. The parasitoid pupae were exposed to pesticide residues on fresh olive tree leaves at recommended concentrations (RC) at different time intervals: 3, 10, 17, 24 and 31 days after pesticide applications. Parasitism viability (% emergence from parasitized eggs) and adult emergence time (developmental time from pupa to adult emergence) were evaluated. Regarding to the International Organization of Biological Control (IOBC) guidelines, results of toxicity effects of insecticides show that: Deltamethrin was moderately harmful to all Trichogramma species at RC (Decis®100 milliliters·ha−1), however, spinosad was harmless to moderately harmful at RC (Tracer®20 milliliters·ha−1). Trichogramma species revealed differences with regard to adult emergence time and exhibited significant changes in parasitism viability with increasing time after pesticide treatment. While deltamethrin residues affected parasitism viability 31 days after the product application, spinosad displayed similar viability for almost species 24 days after the application. The usefulness of Trichogramma parasitoids used as biological control agents, in olive tree ecosystem, was discussed in integrated pest management programs for Prays oleae control when parasitoid species were exposed during pupal stage to the insecticide residue.展开更多
Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator...Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator superfamily(MFS)targeting carbohydrates represent the largest family of sugar transporters in many plants.Strawberry(Fragaria×ananassa Duchesne)is an important crop appreciated worldwide for its unique fruit flavor.The involvement of MFS sugar transporters(STs)in cultivated strawberry fruit sugar accumulation is largely unknown.In this work,we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 varieties.Then,a total of 67 ST genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of F.vesca,the dominant subgenome provider for modern cultivated strawberry.Phylogenetic analysis updated the nomenclature of strawberry ST homoeologs.Both the chromosomal distribution and structural characteristics of the ST family were improved.Semi-RT-PCR analysis in nine tissues from cv.Benihoppe screened 34 highly expressed ST genes in fruits.In three varieties with dramatically differing fruit sugar levels,qPCR integrated with correlation analysis between ST transcript abundance and sugar content identified 13 sugar-correlated genes.The correlations were re-evaluated across 19 varieties,including major commercial cultivars grown in China.Finally,a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was proposed.Our work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.展开更多
The disease symptoms recognized as‘Anthracnose’are caused by Colletotrichum spp.and lead to large-scale strawberry(Fragaria×ananassa Duchesne)losses worldwide in terms of both quality and production.Little is k...The disease symptoms recognized as‘Anthracnose’are caused by Colletotrichum spp.and lead to large-scale strawberry(Fragaria×ananassa Duchesne)losses worldwide in terms of both quality and production.Little is known regarding the mechanisms underlying the genetic variations in the strawberry–Colletotrichum spp.interaction.In this work,Colletotrichum gloeosporioides(C.gloeosporioides)infection was characterized in two varieties exhibiting different susceptibilities,and the involvement of salicylic acid(SA)was examined.Light microscopic observation showed that C.gloeosporioides conidia germinated earlier and faster on the leaf surface of the susceptible cultivar compared with the less-susceptible cultivar.Several PR genes were differentially expressed,with higher-amplitude changes observed in the less-susceptible cultivar.The less-susceptible cultivar contained a higher level of basal SA,and the SA levels increased rapidly upon infection,followed by a sharp decrease before the necrotrophic phase.External SA pretreatment reduced susceptibility and elevated the internal SA levels in both varieties,which were sharply reduced in the susceptible cultivar upon inoculation.The less-susceptible cultivar also displayed a more sensitive and marked increase in the transcripts of NB-LRR genes to C.gloeosporioides,and SA pretreatment differentially induced transcript accumulation in the two varieties during infection.Furthermore,SA directly inhibited the germination of C.gloeosporioides conidia;NB-LRR transcript accumulation in response to SA pretreatment was both dose-and cultivar-dependent.The results demonstrate that the less-susceptible cultivar showed reduced conidia germination.The contribution of SA might involve microbial isolate-specific sensitivity to SA,cultivar/tissue-specific SA homeostasis and signaling,and the sensitivity of R genes and the related defense network to SA and pathogens.展开更多
Assessment of quality of biological control agents is an important step for the success of any biocontrol program.Experiments were carried out to select candidate Trichogramma species for use in biological control of ...Assessment of quality of biological control agents is an important step for the success of any biocontrol program.Experiments were carried out to select candidate Trichogramma species for use in biological control of the olive moth Prays oleae Bern(Lepidoptera,Yponomeutidae).Thirteen local strains were preselected on the basis of the geographical origin and the availability from mass production of the rearing host Ephestia kuehniella ZELL.(Lepidoptera,Pyralidae) under laboratory conditions.Selection tests were focusing on acceptance and preference for P.oleae eggs.In the acceptance test,a single parasitoid female was offered P.oleae eggs on "tricho-card" for 6 hours at 25°C,70% HR and L16:D9 and assessed for parasitism after 6 days.The preference was determined by offering the choice and observing contacts with eggs of the target pest P.oleae and eggs of the host E.kuehniella.Parasitism was therefore assessed 6 days after the experiment under similar laboratory conditions.In host acceptance test,most eggs of P.oleae were parasited by strains belonging to the species T.oleae,T.bourarachae and T.cacoeciae.These strains also showed a high number of contacts with olive moth eggs in preference tests.However,a small number of T.bourarachae observed ovipositions resulted in successful parasitism.Therefore,T.oleae and T.cacoeciae were selected as candidates for further experiments.展开更多
Through analysis of trap catches and fruit decay rate of different pear varieties,it was found that there were significant differences in parasitization of Grapholitha molesta（ Busck） among different pear varieties....Through analysis of trap catches and fruit decay rate of different pear varieties,it was found that there were significant differences in parasitization of Grapholitha molesta（ Busck） among different pear varieties. The trap catches of Huangguan pear was the highest; the fruit decay rate of Xueqing pear was extremely higher than those of other varieties; the trap catches of early red comice were significantly lower than those of other varieties,and no injured fruits were found.展开更多
Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by Candidatus Liberibacter spp., which is vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Secretor...Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by Candidatus Liberibacter spp., which is vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Secretory proteins are important in bacterial pathogenesis and structure components. Some of them are expressed at a high level. To obtain the highly-expressed secretory protein genes (SPGs) for antiserum preparation, six candidate SPGs were chosen from Candidatus Liberibacter asiaticus by bioinformatic analysis and were further tested by qPCR and RT-qPCR methods, respectively. The result showed that two SPGs, 408 and pap (both are Flp pilus assembly protein genes), have relative high amounts of DNA and RNA transcripts of early HLB-infected green orange leaves. The 408 and pap genes were further constructed into the plant expression vector pCAMBIA1300 (GV1300: GFP) and expressed in tobacco leaf epidermal cells for subcellular localization analysis. The transient expression results indicated that the 408 protein is located in the nuclei and cytoplasm of tobacco leaf cells. However, the pap protein is located in the cytoplasm of tobacco leaf cells, which may help the pathogen invade into plant cells. This research is an important foundation for the preparation of the antiserum against Candidatus Liberibacter asiaticus and the early detection of HLB disease.展开更多
‘Chinese Cling’is an important founder in peach breeding history due to the pleasant flavor.Genome-wide association studies(GWAS)combined with genomic selection are promising tools in fruit tree breeding,as there is...‘Chinese Cling’is an important founder in peach breeding history due to the pleasant flavor.Genome-wide association studies(GWAS)combined with genomic selection are promising tools in fruit tree breeding,as there is a considerable time lapse between crossing and release of a cultivar.In this study,242 peaches from Shanghai germplasm were genotyped with 145456 single-nucleotide polymorphisms(SNPs).The six agronomic traits of fruit flesh color,fruit shape,fruit hairiness,flower type,pollen sterility,and soluble solids content,along with 14 key volatile odor compounds(VOCs),were recorded for multiple-statistical GWAS.Except the reported candidate genes,six novel genes were identified as associated with these traits.Thirty-nine significant SNPs were associated with eight VOCs.The putative candidate genes were confirmed for VOCs by RNA-seq,including three genes in the biosynthesis pathway found to be associated with linalool,soluble solids content,and cis-3-hexenyl acetate.Multiple-trait genomic prediction enhanced the predictive ability forγ-decalactone to 0.7415 compared with the single-trait model value of 0.1017.One PTS1-SSR marker was designed to predict the linalool content,and the favorable genotype 187/187 was confirmed,mainly existing in the‘Shanghai Shuimi’landrace.Overall,our findings will be helpful in determining peach accessions with the ideal phenotype and show the potential of multiple-trait genomic prediction to improve accuracy for highly correlated genetic traits.The diagnostic marker will be valuable for the breeder to bridge the gap between quantitative trait loci and marker-assisted selection for developing strong-aroma cultivars.展开更多
Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bear...Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bearing period. It is propagated mainly through grafting,and is suitable for popularization and application in the landscape of the Yangtze River basin and north of the Yangtze River.展开更多
The determination of site productivity in forest ecosystems plays a crucial role in resource management.This study was carried out to identify relationships between site characteristics and height growth of Corsican m...The determination of site productivity in forest ecosystems plays a crucial role in resource management.This study was carried out to identify relationships between site characteristics and height growth of Corsican maritime pine(Pinus pinaster Ait.)plantations in Turkey.Sixty-nine sample plots>20 years of age were selected from locations with different inclinations,aspects,elevations,slope positions and site class.Soil samples were taken at various depths.Height and age were measured on a dominant tree after felling in each plot.Physical and chemical properties of the soil were determined.Relationships between site index(SI 25)and physiographic factors,climatic attributes as well as soil properties were evaluated using correlation analysis and multiple regression analysis.Site index was significantly related with annual precipitation,mean spring rainfall,rainfall June to September,rainfall of the driest month,length of the dry period,mean maximum temperature,mean temperature of the warmest month,stoniness of the soil,sand,silt,clay,pH,electrical conductivity,and available water capacity.Multiple regression accounted for 57.9%of variations in height growth.The models obtained can be used to determine the site index of potential areas in Turkey for maritime pine.It can be said that the productivity of maritime pine may decline in the future due to global climate change.展开更多
In the Tunisian arid zones, where the soils are sandy and very poor in organic and mineral matters, wind erosion process is very active and triggered the departure of the thinnest elements of the soil and the appariti...In the Tunisian arid zones, where the soils are sandy and very poor in organic and mineral matters, wind erosion process is very active and triggered the departure of the thinnest elements of the soil and the apparition of dune building. Facing to these situations, many simple and efficient practices for combating desertification had been tested since many years in the Tunisian arid zones. The mulching of the olive mill wastewaters is one of these techniques. Otherwise, an olive mill wastewaters spray experimental device, with these respective doses of 50 m3/ha, 100 m3/ha and 200 m3/ha next to a witness parcel without OMW, has been putted in place since 1995 in the Chammakh-Zarzis, Tunisia. The mulching of the OMW, in the southern Tunisian regions constitutes an interesting alternative for the controlled and rational evacuation of this polluting sewage on the one hand and for the soil cohesion improvement by： （i） the increase of the organic matter rate which raised, in proportion to the dose, from 0.06% to 1.27% after 10 years of OMW mulching, and （ii） the improvement of the soil structural stability or ~＇the mean weight diameter＂ where the aggregation rate superior to 2 mm is increased until 34% with the dose of 200 m3/ha. Therefore, that treatment will bind the sand particles and thereof control of sandstorms is gained. Indeed, the results in wind tunnel tests on the soil treated with these different doses of OMW showed that the threshold friction velocity u,t was raised from 8.5 m/s to 12 m/s for 50 m3/ha and 200 m3/ha, respectively.展开更多
[Objectives]To protect the local rare citrus germplasm resources and further develop and cultivate new citrus varieties.[Methods]The germplasm resources of Shatangju(Citrus flamea Hort.Ex Tseng Shiyueju)in Sihui City ...[Objectives]To protect the local rare citrus germplasm resources and further develop and cultivate new citrus varieties.[Methods]The germplasm resources of Shatangju(Citrus flamea Hort.Ex Tseng Shiyueju)in Sihui City of Guangdong Province and other 8 citrus varieties from the same origin were evaluated.The appearance characteristics of tree shape,the length and thickness of shoot at each shoot stage,the appearance characteristics of leaves and the fruit quality of each variety were investigated.[Results]Through the comparison of various citrus varieties grafted with sour mandarin,the results showed that the overall advantages of the length and thickness of the shoots of Shatang tangerine in summer and autumn were more prominent,which was conducive to fruit setting.The sprouting time of the three shoots of each variety was different,and there were sporadic sprouting phenomena in different seasons,so the growth was different,and the length and thickness of the shoots were different in different seasons.There were significant differences in shoot length and diameter among different varieties in the same season.The coefficient of variation of Shatangju's leaves was low,and the traits were stable;leaf shape,leaf base shape,leaf margin and other indicators among the three shoots of the same variety and the differences among the same shoots of different varieties were compared.All varieties had strong flavor,separately sweet and sour,sweet and sweet taste.Shatangju has many segments,orange pericarp,rough texture,easy peeling,and high oblate fruit shape.The orange pericarp was favored by the participants,while the rough and thick peel was not favored by the participants.[Conclusions]Based on the improvement of local varieties,it is recommended to improve the smoothness of pericarp,reducing the thickness of pericarp and improving the flavor of fruit will be conducive to increasing sales.In the cultivation process of various quality resources,especially in the period of fruit growth and development,the plan of nutrition and fertilization should be formulated according to the rules of fruit growth and development,the rules of yield formation and the rules of quality composition,so as to establish the integrated nutrient management system of various varieties.展开更多
文摘The study was carried out in northTunisia(36.5?N, 10.2?E) in order to get a comprehensive view of the growth dynamic of young olive orchards (Olea europaea L.,). The experiment involved irrigated trees of cultivars Chétoui, Manzanille, Meski and Picholine, planted at 6 ×6 m2spacing. Tree height, shoot length and canopy, fruit and trunk diameters were monitored regularly after plantation on 12 trees per variety. Root development was analyzed on Chétoui trees, only. Growth patterns were established annually for each variety before proposing an average model for each growth parameter. Results showed that tree height, shoot length and trunk diameter grew following an S-shaped curve with maximum annual increases occurring on the 4th year for tree height and a year later for canopy. The minimum gain coincided with the highest fruit load year, indicating that competition for assimilates concerns also young trees. Average growth patterns for tree height and shoots showed sustained rates all over the growing season with seven distinct periods of growth. Rapid growth occurred in April, July, and September, with similar growth trends observed for productive and less productive cultivars. However, the studied varieties behaved differently. Picholine cv., provided the most important increases and was the best water user. Results also showed that most roots were confined to the top soil layers and developed nearby the trunks. High root densities and important water depletion were observed in this area and thus, water and fertilizers should be supplied for young trees at these depths and distances from trunks. Root and canopy development were highly correlated (r = 0.94) and interfered with fruit growth. When trees set their first productions, the root-canopy ratio approximated the unit. An optimum ratio between root length and leaf area was found (2.3 km·m?2) for the 6-year-old tree, indicating good equilibrium between the above and the underground parts. On the basis of these results, a mathematical model was developed allowing a precise estimation of water requirements of olive trees during a period, where ground cover rarely exceeds 30%. We can conclude that all these models, graphic and mathematic give precise information on the occurrence of the various phenophases of young olive trees and may be used for a quantitative appraisal of the performance of olive varieties under a given environment. However, some aspects would be probed deeper and particularly the influence of climatic data on growth dynamic.
文摘In the present article it will be critically questioned the traditional entrepreneurship education approaches based on a narrow conception of competency, and their values. Assuming the perspective that to be an entrepreneur is basically an attitude towards life and the world, there proposed holistic, constructivist and experiential processes and strategies for entrepreneurship education. The ＂entrepreneur XXI＂, must be able to undertake a social function of change, so, an economical and social development more human, ethical and intelligent. Under this assumption, the ＂Tree Model for the Development of Entrepreneurial Competencies＂, that will be discussed globally in the second part of this article, suggests a dynamic and experiential approach ofentrepreneurship education based on the qualification of people＇s behaviour, self-esteem, competencies and experiences; a profile of key behavioural and performance competencies （root）, experimental pedagogical procedures （trunk） and real results within group projects （fruits）. This model has been developed during the last decade （2001-2011）, using a multidisciplinary research-action procedure, within business, education （at different teaching levels） and social project environments.
文摘Magnesium is an essential nutrient element for the growth and development of fruit trees.It not only participates in photosynthesis of plants,but also promotes physiological and biochemical reactions such as carbohydrate and protein synthesis,enzyme activation,and reactive oxygen species(ROS)metabolism.Magnesium deficiency in fruit trees will directly influence the growth and development of trees and the improvement of fruit quality.This study mainly reviews the nutritional and physiological mechanism of magnesium in plants and the nutritional diagnosis of magnesium deficiency in fruit trees,in order to provide a theoretical basis for further research on magnesium nutrition in fruit trees,and accordingly provide a certain reference for the application of magnesium fertilizer.
文摘This paper analyzes the current situation of training young scientific and technological talents,attracting talents and making good use of talents in Changli Fruit Tree Research Institute of Hebei Academy of Agricultural and Forestry Sciences.There are some problems,such as lack of talent recruitment planning,and lack of follow-up training mechanism.This paper puts forward some measures,such as formulating a scientific recruitment plan,making a follow-up plan for talent training,building an ability-and performance-oriented evaluation mechanism for scientific and technological talents,so as to attach importance to the training of young scientific research talents and promote the echelon construction of the research institute.
基金Supported by the Project of Human Resources and Social Security in Hebei Province(JRS-2022-3102)。
文摘From two aspects:the composition of scientific research management institutions and the tasks undertaken by scientific research management departments,the present situation of scientific and technological management team in agricultural research institutes is expounded.Some problems existed in the scientific and technological management team of provincial agricultural research institutes are analyzed,such as uneven knowledge structure of scientific research management personnel,low degree of specialization,poor stability,lack of professional and systematic job training,etc.Professional development countermeasures are proposed,such as optimizing the structure of scientific research management team,establishing a professional management system with the characteristics of agricultural scientific research institutions,formulating corresponding evaluation mechanism for scientific research management personnel,and strengthening the training of agricultural scientific research management personnel.
基金Supported by the Project of Human Resources and Social Security in Hebei Province(JRS-2022-3102)。
文摘Current situation of introduction,cultivation and use of high-level talents in agricultural research institutes are analyzed,and the optimized development strategy of further strengthening the construction,introduction,cultivation and use of high-level talent team is thought.It plays a positive role in provincial agricultural research institutes discovering,cultivating and gathering a number of high-quality talents and high-level innovation teams.
基金supported by the National Natural Science Foundation of China (31601731)
文摘Based on the recently published whole-genome sequence of cultivated strawberry ’Camarosa’, in this study, 222FaWRKY genes were identified in the ’Camarosa’ genome. Phylogenetic analysis showed that the 222 FaWRKY candidate genes were classified into three groups, of which 41 were in group Ⅰ, 142 were in group Ⅱ, and 39 were in group Ⅲ. The 222 FaWRKY genes were evenly distributed among the seven chromosomes. The exon–intron structures and motifs of the WRKY genes had evolutionary diversity in different cultivated strawberry genomes. Regarding differential expression, the expression of FaWRKY133 was relatively high in leaves, while FaWRKY63 was specifically expressed in roots. FaWRKY207, 59, 46, 182, 156, 58, 39, 62 and 115 were up-regulated during achene development from the green to red fruit transition. FaWRK181, 166 and 211 were highly expressed in receptacles at the ripe fruit stage. One interesting finding was that Fa WRKY179 and 205 were significantly repressed after Colletotrichum fructicola inoculation in both ’Benihoppe’ and ’Sweet Charlie’ compared with Mock. The data reported here provide a foundation for further comparative genomics and analyses of the distinct expression patterns of FaWRKY genes in various tissues and in response to C. fructicola inoculation.
基金support from the Natural Science Foundation of China(31972364)Yuyao Forestry Technology Extension Center.H.M.J.was supported by the National Natural Science Foundation of China(31901985).Z.S.G.,Y.J.,C.Y.C.were funded by a Ningbo Science and Technology Research Grant(contract no 2016C10021).
文摘Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the femalespecific marker MrFT2_BD-SEX.The integrated map included eight linkage groups and spanned 491 cM.Eleven sexassociated markers were identified,six of which were located in linkage group 8,in agreement with the previously reported location of the sex-determining region.The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions.Among the females of the accessions,we found two female-specific alleles,designated W-b(151 bp)and W-d(129 bp).We previously found that‘Dongkui’,a female cultivar,could produce viable pollen(we refer to such plants‘Dongkui-male’)and serve as the paternal parent in crosses.The genotypes of the MrFT2_BD-SEX marker were W-b/Z in‘Biqi’and W-d/Z in‘Dongkui-male’.The progeny of a cross between these parents produced a 3:1 female(W-)to male(ZZ)ratio and the expected 1:1:1:1 ratio of W-b/W-d:W-b/Z:W-d/Z:Z/Z.In addition,the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes.Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus(W)in a small genomic region(59 kb and less than 3.3 cM).Furthermore,we have produced a homozygous“super female”(WW)that should produce all-female offspring in the F2 generation,providing a foundation for commercial use and presenting great potential for use in modern breeding programs.
文摘In order to explore the extraction method of carotenoids from citrus leaves,the extraction effect of carotenoids from leaves of Honey pomelo by ultrasonic-assisted extraction was investigated by single factor experiment and orthogonal experiment.The results showed that the optimum extraction condition was:the ration of ethanol and acetone 1∶1(v/v,containing 0.1%BHT),solid-liquid ratio 1∶30(g/mL),extraction time 7 min,ultrasonic power 240 W,extraction frequency 3 times.Under the above condition,the extraction rate of carotenoids was 85.98%.
文摘This study investigated the direct effect of the insecticides deltamethrin and spinosad on three egg parasitoids species: Trichogramma oleae, T. cacoeciae and T. bourarachae. The parasitoid pupae were exposed to pesticide residues on fresh olive tree leaves at recommended concentrations (RC) at different time intervals: 3, 10, 17, 24 and 31 days after pesticide applications. Parasitism viability (% emergence from parasitized eggs) and adult emergence time (developmental time from pupa to adult emergence) were evaluated. Regarding to the International Organization of Biological Control (IOBC) guidelines, results of toxicity effects of insecticides show that: Deltamethrin was moderately harmful to all Trichogramma species at RC (Decis®100 milliliters·ha−1), however, spinosad was harmless to moderately harmful at RC (Tracer®20 milliliters·ha−1). Trichogramma species revealed differences with regard to adult emergence time and exhibited significant changes in parasitism viability with increasing time after pesticide treatment. While deltamethrin residues affected parasitism viability 31 days after the product application, spinosad displayed similar viability for almost species 24 days after the application. The usefulness of Trichogramma parasitoids used as biological control agents, in olive tree ecosystem, was discussed in integrated pest management programs for Prays oleae control when parasitoid species were exposed during pupal stage to the insecticide residue.
基金funded by Shanghai Agriculture Applied Technology Development Programs,China(Grant No.G2014070202 and No.G2019-02-08-00-08-F01108).
文摘Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator superfamily(MFS)targeting carbohydrates represent the largest family of sugar transporters in many plants.Strawberry(Fragaria×ananassa Duchesne)is an important crop appreciated worldwide for its unique fruit flavor.The involvement of MFS sugar transporters(STs)in cultivated strawberry fruit sugar accumulation is largely unknown.In this work,we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 varieties.Then,a total of 67 ST genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of F.vesca,the dominant subgenome provider for modern cultivated strawberry.Phylogenetic analysis updated the nomenclature of strawberry ST homoeologs.Both the chromosomal distribution and structural characteristics of the ST family were improved.Semi-RT-PCR analysis in nine tissues from cv.Benihoppe screened 34 highly expressed ST genes in fruits.In three varieties with dramatically differing fruit sugar levels,qPCR integrated with correlation analysis between ST transcript abundance and sugar content identified 13 sugar-correlated genes.The correlations were re-evaluated across 19 varieties,including major commercial cultivars grown in China.Finally,a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was proposed.Our work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.
基金This work was supported by funds from Zhejiang Provincial Natural Science Foundation of China(LQ12C02001)the Science and Technology Commission of Shanghai Municipality(Natural Science Foundation,10ZR1426700,Key Program,12391901400,Key Basic Research Project,14JC1405400)the Agricultural Commission of Shanghai Municipality(Key program,2012-No.1–3,youth fund,2014-No.1–28).
文摘The disease symptoms recognized as‘Anthracnose’are caused by Colletotrichum spp.and lead to large-scale strawberry(Fragaria×ananassa Duchesne)losses worldwide in terms of both quality and production.Little is known regarding the mechanisms underlying the genetic variations in the strawberry–Colletotrichum spp.interaction.In this work,Colletotrichum gloeosporioides(C.gloeosporioides)infection was characterized in two varieties exhibiting different susceptibilities,and the involvement of salicylic acid(SA)was examined.Light microscopic observation showed that C.gloeosporioides conidia germinated earlier and faster on the leaf surface of the susceptible cultivar compared with the less-susceptible cultivar.Several PR genes were differentially expressed,with higher-amplitude changes observed in the less-susceptible cultivar.The less-susceptible cultivar contained a higher level of basal SA,and the SA levels increased rapidly upon infection,followed by a sharp decrease before the necrotrophic phase.External SA pretreatment reduced susceptibility and elevated the internal SA levels in both varieties,which were sharply reduced in the susceptible cultivar upon inoculation.The less-susceptible cultivar also displayed a more sensitive and marked increase in the transcripts of NB-LRR genes to C.gloeosporioides,and SA pretreatment differentially induced transcript accumulation in the two varieties during infection.Furthermore,SA directly inhibited the germination of C.gloeosporioides conidia;NB-LRR transcript accumulation in response to SA pretreatment was both dose-and cultivar-dependent.The results demonstrate that the less-susceptible cultivar showed reduced conidia germination.The contribution of SA might involve microbial isolate-specific sensitivity to SA,cultivar/tissue-specific SA homeostasis and signaling,and the sensitivity of R genes and the related defense network to SA and pathogens.
基金INCODEV (TRIPHELIO) (UE/ 2002-2005) :Sustainable Control of Lepidopterous Pests in Olive Groves - Integration of Egg Parasitoids and Pheromones
文摘Assessment of quality of biological control agents is an important step for the success of any biocontrol program.Experiments were carried out to select candidate Trichogramma species for use in biological control of the olive moth Prays oleae Bern(Lepidoptera,Yponomeutidae).Thirteen local strains were preselected on the basis of the geographical origin and the availability from mass production of the rearing host Ephestia kuehniella ZELL.(Lepidoptera,Pyralidae) under laboratory conditions.Selection tests were focusing on acceptance and preference for P.oleae eggs.In the acceptance test,a single parasitoid female was offered P.oleae eggs on "tricho-card" for 6 hours at 25°C,70% HR and L16:D9 and assessed for parasitism after 6 days.The preference was determined by offering the choice and observing contacts with eggs of the target pest P.oleae and eggs of the host E.kuehniella.Parasitism was therefore assessed 6 days after the experiment under similar laboratory conditions.In host acceptance test,most eggs of P.oleae were parasited by strains belonging to the species T.oleae,T.bourarachae and T.cacoeciae.These strains also showed a high number of contacts with olive moth eggs in preference tests.However,a small number of T.bourarachae observed ovipositions resulted in successful parasitism.Therefore,T.oleae and T.cacoeciae were selected as candidates for further experiments.
基金Supported by Youth Fund of Hebei Academy of Agriculture and Forestry(A2015020106)
文摘Through analysis of trap catches and fruit decay rate of different pear varieties,it was found that there were significant differences in parasitization of Grapholitha molesta（ Busck） among different pear varieties. The trap catches of Huangguan pear was the highest; the fruit decay rate of Xueqing pear was extremely higher than those of other varieties; the trap catches of early red comice were significantly lower than those of other varieties,and no injured fruits were found.
文摘Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by Candidatus Liberibacter spp., which is vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Secretory proteins are important in bacterial pathogenesis and structure components. Some of them are expressed at a high level. To obtain the highly-expressed secretory protein genes (SPGs) for antiserum preparation, six candidate SPGs were chosen from Candidatus Liberibacter asiaticus by bioinformatic analysis and were further tested by qPCR and RT-qPCR methods, respectively. The result showed that two SPGs, 408 and pap (both are Flp pilus assembly protein genes), have relative high amounts of DNA and RNA transcripts of early HLB-infected green orange leaves. The 408 and pap genes were further constructed into the plant expression vector pCAMBIA1300 (GV1300: GFP) and expressed in tobacco leaf epidermal cells for subcellular localization analysis. The transient expression results indicated that the 408 protein is located in the nuclei and cytoplasm of tobacco leaf cells. However, the pap protein is located in the cytoplasm of tobacco leaf cells, which may help the pathogen invade into plant cells. This research is an important foundation for the preparation of the antiserum against Candidatus Liberibacter asiaticus and the early detection of HLB disease.
基金This work was supported by funds from the National Key Research and Development Program of China(2019YFD1000801)the Science and Technology Commission of Shanghai Municipality(23 N11900400)+2 种基金Hu Nong Ke Chuang Zi(2021)1-1,Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties,China(2021C02066-4)the Outstanding Team Program of Shanghai Academy of Agricultural Science(2022-004)the‘Pangao’Program of Shanghai Academy of Agricultural Science.
文摘‘Chinese Cling’is an important founder in peach breeding history due to the pleasant flavor.Genome-wide association studies(GWAS)combined with genomic selection are promising tools in fruit tree breeding,as there is a considerable time lapse between crossing and release of a cultivar.In this study,242 peaches from Shanghai germplasm were genotyped with 145456 single-nucleotide polymorphisms(SNPs).The six agronomic traits of fruit flesh color,fruit shape,fruit hairiness,flower type,pollen sterility,and soluble solids content,along with 14 key volatile odor compounds(VOCs),were recorded for multiple-statistical GWAS.Except the reported candidate genes,six novel genes were identified as associated with these traits.Thirty-nine significant SNPs were associated with eight VOCs.The putative candidate genes were confirmed for VOCs by RNA-seq,including three genes in the biosynthesis pathway found to be associated with linalool,soluble solids content,and cis-3-hexenyl acetate.Multiple-trait genomic prediction enhanced the predictive ability forγ-decalactone to 0.7415 compared with the single-trait model value of 0.1017.One PTS1-SSR marker was designed to predict the linalool content,and the favorable genotype 187/187 was confirmed,mainly existing in the‘Shanghai Shuimi’landrace.Overall,our findings will be helpful in determining peach accessions with the ideal phenotype and show the potential of multiple-trait genomic prediction to improve accuracy for highly correlated genetic traits.The diagnostic marker will be valuable for the breeder to bridge the gap between quantitative trait loci and marker-assisted selection for developing strong-aroma cultivars.
基金Supported by Innovation Projects of Wuhan Academy of Agricultural Sciences(Cx201317Cxfzx201506Cxtd201507)
文摘Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bearing period. It is propagated mainly through grafting,and is suitable for popularization and application in the landscape of the Yangtze River basin and north of the Yangtze River.
基金funded by the Turkish General Directorate of Forestry as part of the project“Relationship between growth of maritime pine(Pinus pinaster Ait.)plantations and site characteristics in Turkey[ESK‒27(6319)]”.
文摘The determination of site productivity in forest ecosystems plays a crucial role in resource management.This study was carried out to identify relationships between site characteristics and height growth of Corsican maritime pine(Pinus pinaster Ait.)plantations in Turkey.Sixty-nine sample plots>20 years of age were selected from locations with different inclinations,aspects,elevations,slope positions and site class.Soil samples were taken at various depths.Height and age were measured on a dominant tree after felling in each plot.Physical and chemical properties of the soil were determined.Relationships between site index(SI 25)and physiographic factors,climatic attributes as well as soil properties were evaluated using correlation analysis and multiple regression analysis.Site index was significantly related with annual precipitation,mean spring rainfall,rainfall June to September,rainfall of the driest month,length of the dry period,mean maximum temperature,mean temperature of the warmest month,stoniness of the soil,sand,silt,clay,pH,electrical conductivity,and available water capacity.Multiple regression accounted for 57.9%of variations in height growth.The models obtained can be used to determine the site index of potential areas in Turkey for maritime pine.It can be said that the productivity of maritime pine may decline in the future due to global climate change.
文摘In the Tunisian arid zones, where the soils are sandy and very poor in organic and mineral matters, wind erosion process is very active and triggered the departure of the thinnest elements of the soil and the apparition of dune building. Facing to these situations, many simple and efficient practices for combating desertification had been tested since many years in the Tunisian arid zones. The mulching of the olive mill wastewaters is one of these techniques. Otherwise, an olive mill wastewaters spray experimental device, with these respective doses of 50 m3/ha, 100 m3/ha and 200 m3/ha next to a witness parcel without OMW, has been putted in place since 1995 in the Chammakh-Zarzis, Tunisia. The mulching of the OMW, in the southern Tunisian regions constitutes an interesting alternative for the controlled and rational evacuation of this polluting sewage on the one hand and for the soil cohesion improvement by： （i） the increase of the organic matter rate which raised, in proportion to the dose, from 0.06% to 1.27% after 10 years of OMW mulching, and （ii） the improvement of the soil structural stability or ~＇the mean weight diameter＂ where the aggregation rate superior to 2 mm is increased until 34% with the dose of 200 m3/ha. Therefore, that treatment will bind the sand particles and thereof control of sandstorms is gained. Indeed, the results in wind tunnel tests on the soil treated with these different doses of OMW showed that the threshold friction velocity u,t was raised from 8.5 m/s to 12 m/s for 50 m3/ha and 200 m3/ha, respectively.
基金Supported by China Agricalture Research System of MOF and MARA(CARS-26)Citrus Germplasm Resources Nursery Construction Project in Sihui City of Guangdong Province(KJ-20230310-H010)。
文摘[Objectives]To protect the local rare citrus germplasm resources and further develop and cultivate new citrus varieties.[Methods]The germplasm resources of Shatangju(Citrus flamea Hort.Ex Tseng Shiyueju)in Sihui City of Guangdong Province and other 8 citrus varieties from the same origin were evaluated.The appearance characteristics of tree shape,the length and thickness of shoot at each shoot stage,the appearance characteristics of leaves and the fruit quality of each variety were investigated.[Results]Through the comparison of various citrus varieties grafted with sour mandarin,the results showed that the overall advantages of the length and thickness of the shoots of Shatang tangerine in summer and autumn were more prominent,which was conducive to fruit setting.The sprouting time of the three shoots of each variety was different,and there were sporadic sprouting phenomena in different seasons,so the growth was different,and the length and thickness of the shoots were different in different seasons.There were significant differences in shoot length and diameter among different varieties in the same season.The coefficient of variation of Shatangju's leaves was low,and the traits were stable;leaf shape,leaf base shape,leaf margin and other indicators among the three shoots of the same variety and the differences among the same shoots of different varieties were compared.All varieties had strong flavor,separately sweet and sour,sweet and sweet taste.Shatangju has many segments,orange pericarp,rough texture,easy peeling,and high oblate fruit shape.The orange pericarp was favored by the participants,while the rough and thick peel was not favored by the participants.[Conclusions]Based on the improvement of local varieties,it is recommended to improve the smoothness of pericarp,reducing the thickness of pericarp and improving the flavor of fruit will be conducive to increasing sales.In the cultivation process of various quality resources,especially in the period of fruit growth and development,the plan of nutrition and fertilization should be formulated according to the rules of fruit growth and development,the rules of yield formation and the rules of quality composition,so as to establish the integrated nutrient management system of various varieties.
