Objective:To explore the clinical effects of neurology nursing during the rehabilitation process of stroke patients.Methods:A total of 80 inpatients with stroke,admitted between July 2023 and July 2024,were selected f...Objective:To explore the clinical effects of neurology nursing during the rehabilitation process of stroke patients.Methods:A total of 80 inpatients with stroke,admitted between July 2023 and July 2024,were selected for the study.They were randomly divided into two groups of 40 patients each.The control group received conventional nursing care,while the study group was provided with neurology nursing.The nursing outcomes of the two groups were compared in terms of:(1)rehabilitation progress,(2)nursing satisfaction,(3)psychological status,and(4)self-care ability.Results:The nursing efficacy and satisfaction rates in the study group were significantly higher than those in the control group(P<0.05).Pre-intervention psychological scores showed no significant differences between the two groups(P>0.05).Post-intervention,negative emotions in both groups were alleviated,with the study group showing greater improvement compared to the control group(P<0.05).Additionally,the self-care ability scores of the study group were significantly higher than those of the control group(P<0.05).Conclusion:Neurology nursing during the rehabilitation process of stroke patients demonstrated ideal clinical effects,positively contributing to patient recovery,alleviating negative psychological states,and proving beneficial for promotion.展开更多
Background:Lung cancer remains the primary cause of mortality worldwide.Methylation modifications of eukaryotic messenger RNA(mRNA),recognized as one of the most prevalent chemical alterations,significantly impact the...Background:Lung cancer remains the primary cause of mortality worldwide.Methylation modifications of eukaryotic messenger RNA(mRNA),recognized as one of the most prevalent chemical alterations,significantly impact the stability,splicing,and translation of mRNA.Methyltransferase-like 21A(METTL21A)functions as a non-histone methyltransferase.The role of methylation-related compounds in the development of cancer has garnered increasing interest in recent years.Methods:The expression levels of METTL21A were assessed in 86 lung cancer samples and 78 adjacent non-cancerous tissues from Taizhou Hospital.Gene expression data were sourced from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases.Multi-omics studies were conducted to examine the biological role of METTL21A within lung cancer.We thoroughly explored the potential functions and prognostic implications of METTL21A in this context.Results:An elevated expression of METTL21A was observed in lung cancer tissues.Furthermore,high levels of METTL21A expression correlate with various factors,including age,sex,race,tumor protein P53(TP53)mutations,cancer type,metastasis,and the pathological stage of lung cancer patients,indicating a relationship with poor prognosis.Additionally,METTL21A may affect lung cancer patient outcomes through distinct patterns of immune cell infiltration.Conclusion:METTL21A emerges as a promising candidate prognostic biomarker linked to immune invasion in lung cancer.展开更多
Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) share a common route of transmission so that about one third of HIV infected individuals show HCV coinfection. Highly active antiretroviral therapy has of...Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) share a common route of transmission so that about one third of HIV infected individuals show HCV coinfection. Highly active antiretroviral therapy has offered a longer and better life to infected patients. While has removed AIDS-related diseases from the list of most common causes of death their place has been taken by complications of HCV infection, such as cirrhosis, end stage liver disease and hepatocellular carcinoma (HCC). HIV/HCV co-infection requires complex management, especially when HCC is present. Co-infected patients with HCC undergo the same therapeutic protocol as their mono-infected counterparts, but special issues such as interaction between regimens, withdrawal of therapy and choice of immunosuppressive agents, demand a careful approach by specialists. All these issues are analyzed in this minireview.展开更多
Hepatocellular carcinoma(HCC)is the most frequent primary liver malignancy and the third cause of cancer-related death in the Western Countries.The well-established causes of HCC are chronic liver infections such as h...Hepatocellular carcinoma(HCC)is the most frequent primary liver malignancy and the third cause of cancer-related death in the Western Countries.The well-established causes of HCC are chronic liver infections such as hepatitis B virus or chronic hepatitis C virus,nonalcoholic fatty liver disease,consumption of aflatoxins and tobacco smocking.Clinical presentation varies widely;patients can be asymptomatic while symptomatology extends from right upper abdominal quadrant paint and weight loss to obstructive jaundice and lethargy.Imaging is the first key and one of the most important aspects at all stages of diagnosis,therapy and follow-up of patients with HCC.The Barcelona Clinic Liver Cancer Staging System remains the most widely classification system used for HCC management guidelines.Up until now,HCC remains a challenge to early diagnose,and treat effectively;treating management is focused on hepatic resection,orthotopic liver transplantation,ablative therapies,chemoembolization and systemic therapies with cytotocix drugs,and targeted agents.This review article describes the current evidence on epidemiology,symptomatology,diagnosis and treatment of hepatocellular carcinoma.展开更多
The liver is a solid organ with a wide variety of primary benign or malignant tumors as well as metastatic lesions.Surgical resection of these tumors remains the only curative modality.Several limitations,however,do n...The liver is a solid organ with a wide variety of primary benign or malignant tumors as well as metastatic lesions.Surgical resection of these tumors remains the only curative modality.Several limitations,however,do not allow the performance of these operations.This review evaluates the indications and limitations regarding these extended hepatic resections,as well as describing all the manipulations that increase the candidates for such operations.A thorough review of the literature was performed in order to define indications for extended hepatectomy,as well as to present all methods that contribute to increasing the volume of the future remnant liver.The role of portal vein ligation,portal vein embolization,two-stage hepatectomy,and in situ liver transection are evaluated in the setting of indications and results.Extended hepatectomies are a necessity due to oncological reasons.All methods developed in order to increase the volume of the remnant liver are safe and efficient.in situ liver transection is a novel and revolutionary two-step procedure for extended hepatic resections.Further clinical studies are required to estimate long-term results and the oncological basis of this technique.展开更多
BACKGROUND Circulating tumor cells(CTCs)and survivin are indicators for tumor stage and metastasis,as well as epitheliomesenchymal transition,in various cancers,including hepatocellular cancer(HCC).AIM To explore the ...BACKGROUND Circulating tumor cells(CTCs)and survivin are indicators for tumor stage and metastasis,as well as epitheliomesenchymal transition,in various cancers,including hepatocellular cancer(HCC).AIM To explore the potential of survivin-positive CTCs,specifically,as a marker for tumor progression in HCC patients.METHODS We examined the survivin expression pattern in CTCs obtained from 179 HCC patients,and investigated the in vitro effects of survivin silencing and overexpression on the proliferation and invasion of HCC cells.CTC count and survivin expression in patient samples were examined using RNA in situ hybridization.RESULTS All 179 patients were positive for CTC markers,and 94.41%of the CTCs were positive for survivin.The CTC and survivin-positive CTC counts were significantly higher in the HCC patients than in the normal controls,and were significantly associated with tumor stage and degree of differentiation.Further,survivin overexpression was found to induce HepG2 cell proliferation,reduce apoptosis,and improve invasive ability.CONCLUSION Survivin shows upregulated expression(indicative of anti-apoptotic effects)in HCC.Thus,survivin-positive CTCs are promising as a predictor of HCC prognosis and metastasis,and their accurate measurement may be useful for the management of this cancer.展开更多
Sickle cell disease(SCD) is a life-threatening genetic disorder characterized by chronic hemolytic anemia, vascular injury and multiorgan dysfunctions. Over the last few decades, there have been significant improvemen...Sickle cell disease(SCD) is a life-threatening genetic disorder characterized by chronic hemolytic anemia, vascular injury and multiorgan dysfunctions. Over the last few decades, there have been significant improvements in SCD management in Western countries, especially in pediatric population. An early onset of prophylaxis with Penicillin and a proper treatment of the infections have increased the overall survival in childhood. Nevertheless,management of painful episodes and prevention of organ damage are still challenging and more efforts are needed to better understand the mechanisms behind the development of chronic organ damages. Hydroxyurea(Hydroxycarbamide, HU), the only medication approved as a disease-modifying agent by the United States Food and Drug Administration and the European Medicines Agency,is usually under-used, especially in developing countries.Currently, hematopoietic stem-cell transplantation is considered the only curative option, although its use is limited by lack of donors and transplant-related toxicity.SCD symptoms are similar in children and adults, but complications and systemic organ damages increase with age, leading to early mortality worldwide. Experts in comprehensive care of young patients with SCD, especially those approaching the transition age to adulthood,are missing, leading people to rely on urgent care,increasing health care utilization costs and inappropriate treatments. It would be important to establish programs of comprehensive healthcare for patients with SCD from birth to adulthood, to improve their quality and expectancy of life.展开更多
The current study was conducted to determine the epidemiology and antibiotic sensitivity pattern of bacteria isolated from blood of septicemic patients for improved antibiotic therapy. A three-year descriptive study w...The current study was conducted to determine the epidemiology and antibiotic sensitivity pattern of bacteria isolated from blood of septicemic patients for improved antibiotic therapy. A three-year descriptive study was done at Microbiology Laboratory, Ekiti State University Teaching Hospital, Ado Ekiti, from April 2012 to April 2015. Information compiled from patients’ records includes age, sex, isolated organisms and antibiotic susceptibility patterns. Three hundred and thirteen blood cultures were collected from neonatology and pediatrics wards, Out Patients’ Department (OPD) and from other adult patients. Forty one culture plates yielded mono microbial growth (no polymicrobial growth), giving an incidence of 13.1% positive blood culture (N = 41/313). There were 58.4% Gram negative bacilli and 41.6% Gram positive cocci in the microbial growth. Bacteria isolated were Staphylococcus aureus 34% (14/41), Klebsiella species 22% (9/41), Enterococci 17% (7/41), Proteus species 12% (5/41), Escherichia coli 7% (3/41) and Streptococcal pneumoniae 7% (3/41). There was a (35%) higher occurrence of septicemia in neonates than in any other age groups in the hospital. Bacterial sensitivity to 13 antibiotic agents was determined by antibiotics disc diffusion using modified Kirby Bauer’s method. Gram-positive organisms showed a higher antibiotic sensitivity ranging from 14% - 100% than the Gram-negative bacteria (11% - 80%). Staphylococcus aureus and Klebsiella species are the most prevalent organisms. The third generation Cephalosporins (Ceftriaxone) and Floroquinolone (Levofloxacin, Ofloxacin) have proved reliable for management of these blood infections.展开更多
Aim: We demonstrated the risk of developing islet autoantibodies-Insulin Autoanti-bodies (IAAs) and Islets cell Autoantibodies (ICAs)-in type-1 diabetic relatives and newly diagnosed type-1 patients compared to non-di...Aim: We demonstrated the risk of developing islet autoantibodies-Insulin Autoanti-bodies (IAAs) and Islets cell Autoantibodies (ICAs)-in type-1 diabetic relatives and newly diagnosed type-1 patients compared to non-diabetic controls. We also aimed to determine the predictive strengths of both autoantibodies in the development of type-1 diabetes mellitus, and which of the two autoantibodies is a better predictive marker of type-1 diabetes mellitus among Nigerian adults. Methodology: A total number of four hundred and fifty five (455) subjects (211 (46%) males, and 244 (54%) females) aged between 35 - 76 years were recruited for the study. IAA and ICA levels were estimated using ELISA reagents from Biomerica Inc. Other parameters such as fasting blood sugar, urine glucose, and urine protein were assessed using standard biochemical techniques. Results: Relatives of type-1 diabetic patients and newly diagnosed type-1 diabetic patients were at greater risk (p < 0.05) of testing positive for more than one autoantibody (ICA and IAA) compared to non-diabetic controls. In addition, IAAs appeared to be better predictors or markers of type-1 diabetes mellitus compared to ICAs. Conclusion: The present study indicated a greater risk of autoim-mune destruction of the insulin producing beta cells of the pancrease of the type-1 relatives and newly diagnosed type-1 patients and suggests the need for periodic re-cruitment of individuals in the general population, siblings and relatives of type-1 diabetic patients for planned intervention trials. In addition, IAAs appeared to be better autoimmune markers of type-1 diabetes compared to ICAs.展开更多
Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2,broke out in December 2019 in Wuhan city of China and spread rapidly worldwide.Therefore,by March 2020,the World Health Orga...Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2,broke out in December 2019 in Wuhan city of China and spread rapidly worldwide.Therefore,by March 2020,the World Health Organization declared the disease a global pandemic.Apart from the respiratory system,various other organs of the human body are also seriously affected by the virus.Liver injury in patients with a severe form of COVID-19 is estimated to be 14.8%-53.0%.Elevated levels of total bilirubin,aspartate aminotransferase and alanine aminotransferase and low levels of serum albumin and prealbumin are the main laboratory findings.Patients with pre-existing chronic liver disease and cirrhosis are much more prone to develop severe liver injury.This literature review presented the recent scientific findings regarding the pathophysiological mechanisms responsible for liver injury in critically ill patients with COVID-19,the various interactions between drugs used to treat the disease and the function of the liver and the specific tests providing the possibility of early diagnosis of severe liver injury in these patients.Moreover,it highlighted the burden that COVID-19 put on health systems worldwide and its effect on transplant programs and the care provided to critically ill patients in general and particularly to those with chronic liver disease.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has emerged as a major threat to global public health.The virus causes the clinical syndrome known as coronavirus disease 2019(COVID-19),in which multiple org...Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has emerged as a major threat to global public health.The virus causes the clinical syndrome known as coronavirus disease 2019(COVID-19),in which multiple organs can get affected.Apart from manifestations of the respiratory system,which predominate,its clinical presentation is frequently accompanied by symptoms of the gastrointestinal(GI)tract and liver abnormalities.The correlation of symptoms and abnormalities with disease severity is discussed,leading to ambiguous results from international literature.Moreover,the disease infects patients with coexisting liver and GI disorders affecting both their health status and the availability of healthcare services provided to them.The risk of transmission of the disease during aerosol-generating procedures has changed the diagnostic approach and follow-up algorithms for liver and GI diseases.For the safety of both doctors and patients,telemedicine and distant evaluation have become everyday practice,whereas several routines and emergency visits at outpatient and emergency departments have been postponed or delayed.Vaccination against SARS-CoV-2 is underway,providing hope to humanity and the expectation that the post-COVID-19 era is near.This review aims to update knowledge about the manifestations of COVID-19 related to liver and GI diseases and the effect of the pandemic on the diagnostic and therapeutic procedures for these diseases with a special focus on how current practices have changed and what changes will possibly remain in the future.展开更多
We aimed to study the effect of elapsed time from AML diagnosis to treatment (TDT) on OS in a group of patients from public Hospital in Brazil. 41 AML (23 M, 18 F, 41 yrs, 18 - 84 yrs, from 2001 to 2004). There were 3...We aimed to study the effect of elapsed time from AML diagnosis to treatment (TDT) on OS in a group of patients from public Hospital in Brazil. 41 AML (23 M, 18 F, 41 yrs, 18 - 84 yrs, from 2001 to 2004). There were 38 de novo AML and 3 secondary, median TDT was 6 days (range 1 - 82 d);the young ones?were treated earlier than old ones (TDT 4 days vs 11, p = 0.07). Longer TDT (>10 d) was associated with worse CR rates (p = 0.02) and OS (p = 0.04). When patients were categorized into TDT from 1 - 4 d (I) vs >5 (II), those from I presented better OS than II (p = 0.004). When TDT was longer than 7 days OS decreased even more. Hb was higher in patients with TDT I vs II (8.3 vs 7.5 g/dL, p = 0.03) but WBC (p = 0.34) and platelet count (p = 0.75) were not different. Patients with TDT of 10 d were younger than TDT > 10 d (median age 41 vs 70 yrs, p = 0.001). The OS was 15.1% in 2 yrs and 8.6% in 7 yrs. Our data suggest longer TDT, when analyzed continuously, predicted for lower CR rates and OS rates.展开更多
Acute Myeloid Leukemia (AML) is a group of genetically diverse hematopoietic malignancies arising from cell progenitors developing in the myeloid pathway or from primitive stem cells. Genetic susceptibility of AML may...Acute Myeloid Leukemia (AML) is a group of genetically diverse hematopoietic malignancies arising from cell progenitors developing in the myeloid pathway or from primitive stem cells. Genetic susceptibility of AML may account for an increased risk of AML due to partial metabolism of or biocativation of carcinogens. Chemical compounds are metabolized by a two-tiered phase detoxifying system. Polymorphisms in these pathways may lead to DNA damage and development of AML. We determined the frequencies of carcinogen metabolism gene polymorphisms (CYP1A1, del{GSTM1} and del{GSTT1}) in a case control-study based on polymorphism analysis. Fifty-eight consecutively AML patients (median age 62 years) and 174 sex and age-matched control group were assessed by a PCR-RFLP assay. There were 51 de novo and 7 secondary AML. CYP1A1*2A and CYP1A1*2C polymorphisms were more frequent in CG than AML p 0.001 and in contrast, CYP1A1*3 and CYP1A1*4 were more frequent in AML than CG p 0.001. There were no differences in del{GSTM1} neither del{GSTT1} between AML and CG (p = 0.999 and p = 0.539). Odds ratio for AML in patients harboring CYP1A1*3 was 2.36 (95% CI 1.2 - 4.5), 2.38 for CYP1A1*4 (95% CI 0.8 - 6.8). Adjusted OR was 2.63 for CYP1A1*3 (95% CI 1.4 - 5.1) and 2.66 for CYP1A1*4 (95% CI 0.9 - 7.8). In the multivariate analysis CYP1A1*3 polymorphism was a risk factor for AML with an OR for 3.99 (95%CI 1.9 - 8.6). To the best of our knowledge this is the first study to show that CYP1A1*3 heterozygous genotypes increase the risk of AML. Our data support that inherited absence of this carcinogen detoxification pathway may be an important determinant of AML.展开更多
Granulocytic or myeloid sarcoma (MS) is a rare neoplastic condition consisting of a tumor mass of myeloid blasts with or without maturation occurring at an anatomical site other than the bone marrow the association be...Granulocytic or myeloid sarcoma (MS) is a rare neoplastic condition consisting of a tumor mass of myeloid blasts with or without maturation occurring at an anatomical site other than the bone marrow the association between tuberculosis and MS is extremely rare. A 21-year-old female patient presented cough, sore throat and a suppurative swollen gum for 10 days prior to hospital admission. Physical examination revealed moderate pallor and swollen inferior gum. CBC revealed Hb6.5 g/dL, hematocrit 18.4% MCV 97 fL MCH 34 pg, WBC 18.5 ′ 109/μL (1 My/3 Bt/69 Sg/1 Eo/0 Ba/20 Ly/6 Mo), Platelets 43 ′ 109/μL. The peripheral blood smear presented with 3% blast cells (type 1) and granulocytic dysplasia. Bone marrow biopsy showed 100% cellularity. 50% of cells were from granulocytic precursors, diagnosis of granulocytic sarcoma. The diagnosis of AML was established: granulocytic sarcoma with massive gum infiltration (immature granulocytic cells) and 10% of blasts in bone marrow. The patient received induction chemotherapy (3 + 7 daunorubicin 90 mg/m2), and gum tissue culture was positive for Mycobacterium tuberculosis. Simultaneously, a qRT- PCR test confirmed the same bacteria in the gum tissue. Patient treated with isoniazid, rifampicin, pyrazinamide and ethambutol ciprofloxacin and amikacin). Remission was achieved and the patient was submitted for consolidation/ intensification (HiDAC x3) schema and referred to allogeneic HSCT. After induction and full hematological recovery there was no further evidence or recurrence of fever and lytic lesions. Currently patient is under CR and ling follow up (48 months) did not show recurrence of either AML or tuberculosis.展开更多
Ischemic/hypoxic injury significantly damages vascular function,detrimentally impacting patient outcomes.Changes in mitochondrial structure and function are closely associated with ischemia/hypoxia-induced vascular dy...Ischemic/hypoxic injury significantly damages vascular function,detrimentally impacting patient outcomes.Changes in mitochondrial structure and function are closely associated with ischemia/hypoxia-induced vascular dysfunction.The mechanism of this process remains elusive.Using rat models of ischemia and hypoxic vascular smooth muscle cells(VSMCs),we combined transmission electron microscopy,super-resolution microscopy,and metabolic analysis to analyze the structure and function change of mitochondrial cristae.Multi-omics approaches revealed arginase 1(Arg1)upregulation in ischemic VSMCs,confirmed by in vivo and in vitro knockout models showing Arg1's protective effects on mitochondrial cristae,mitochondrial and vascular function,and limited the release of mtDNA.Mechanistically,Arg1 interacting with Mic1o led to mitochondrial cristae remodeling,together with hypoxia-induced VDAC1 lactylation resulting in the opening of MPTP and release of mtDNA of VSMCs.The released mtDNA led to PANoptosis of VSMCs via activation of the cGAS-STING pathway.ChIP-qPCR results demonstrated that lactatemediated Arg1 up-regulation was due to H3K18la upregulation.VSMCs targeted nano-material PLGA-PEl-siRNA@PM-a-SMA(NPsiArg1)significantly improved vascular dysfunction.This study uncovers a new mechanism of vascular dysfunction following ischemic/hypoxic injury:a damaging positive feedback loop mediated by lactate-regulated Arg1 expression between the nucleus and mitochondria,leading to mitochondria cristae disorder and mtDNA release,culminating in VSMCs PANoptosis.Targeting VSMCs Arg1 inhibition offers a potential therapeutic strategy to alleviate ischemia/hypoxia-induced vascular impairments.展开更多
Absolute erythrocytosis,due to an increased production of red blood cells,becomes manifest with hemoglobin(Hb)levels above 165 or 160 g/L or with a hematocrit(HCT)above 51%and 48%in males and females,respectively.1 A ...Absolute erythrocytosis,due to an increased production of red blood cells,becomes manifest with hemoglobin(Hb)levels above 165 or 160 g/L or with a hematocrit(HCT)above 51%and 48%in males and females,respectively.1 A minority of patients have polycythemia vera(PV);acquired secondary erythrocytosis frequently occurs due to appropriate or inappropriate erythropoietin(EPO)excess,while congenital secondary erythrocytosis may derive from genetic defects causing tissue hypoxia.Hereditary erythrocytosis occurs in patients with genetic mutations of the oxygen-sensing pathway(VHL,PHD2,_HIF-1-alpha)or the erythropoietin receptor(EPOR)genes.2 Recently,HFE gene variants have been described in sporadic erythrocytoses.3 In a high proportion(about 70%)of patients with erythrocytosis,a specific etiology remains elusive despite extensive testing,and the diagnosis of idiopathic erythrocytosis(IE)is ruled out.展开更多
In this paper,a sensor using carbon nanospheres modified boron-doped diamond(CNs/BDD)electrode for detecting heavy metal ions rapidly in aqueous solution is studied.The CNs/BDD electrode is characterized systematicall...In this paper,a sensor using carbon nanospheres modified boron-doped diamond(CNs/BDD)electrode for detecting heavy metal ions rapidly in aqueous solution is studied.The CNs/BDD electrode is characterized systematically by techniques including scanning electron microscopy(SEM),X-ray diffractometer(XRD)and X-ray photoelectron spectroscopy(XPS).The electrochemical properties of the CNs/BDD electrode,BDD electrode and Au electrode are compared by cyclic voltammetry.It is found that the CNs/BDD electrode has more negative hydrogen evolution potential,a wider potential window and a larger eff ective electrode area,which is conducive to the accurate detection of heavy metal ions.The square-wave anodic stripping voltammetry(SWASV)is applied to assess and quantify the sensing capability of the CNs/BDD electrode.The eff ects of deposition potential,deposition time and other experimental parameters on the detection performance are studied.Under the optimal experimental conditions,CNs/BDD electrodes exhibit remarkable detection capabilities for lead Pb(Ⅱ)ions,in which a linear relationship within the concentration range from 1 to 140μg/L with a 0.9994 correlation coeffi cient is obtained.Meanwhile,it has excellent repeatability with a low relative standard deviation(RSD)of 0.57%,and the achieved actual limit of detection(LOD)reaches 0.01μg/L.In addition,the interference experiment results show that except for Cd(Ⅱ)ions and Cu(Ⅱ)ions,other common ions in water have little interference to Pb(Ⅱ)ion detection.The Pb(Ⅱ)ions recovery rates are 89.1–115.7%in the real water samples.Experimental analysis indicates that the CNs/BDD electrode possesses the advantages of low detection limit,short detection time,and simple experimental equipment,which meets the requirements of Pb(Ⅱ)ions fi eld detection.展开更多
Background:Patients with extensive burns usually develop pro-coagulation soon after the injury if there is no sepsis occurred.We describe the case of an extensive burn adult suffering from hypocoagulation not related ...Background:Patients with extensive burns usually develop pro-coagulation soon after the injury if there is no sepsis occurred.We describe the case of an extensive burn adult suffering from hypocoagulation not related to sepsis,but secondary to antibiotic treatment.Case presentation:Here,we report a case of an adult male patient suffering from flame burns of 45%total body surface area(40%full thickness)combined with inhalation injury.Hypocoagulopathy with soaring prolonged activated partial thromboplastin time value occurred on third week post-burn while systemic infection had been under control by application of broad-spectrum antibiotics.Investigations showed that not the infection but vitamin K-related coagulation factor deficiency were responsible for unexpected bleeding.However,supplemental vitamin K was not the key as we expected,which prompted us trying to decode the underlying cause of coagulation disturbance in this patient and pick out the most effective treatment for live-saving.After the withdrawal of highly suspected broad-spectrum antibiotic,Meropenem^(■),disturbed vitamin K related coagulation factors gradually restored to their optimal levels so as to maintain normal coagulation status.Therefore,surgical procedures without further risk of bleeding could be carried out in time for wound recovery.The patient was discharged on post-burn day 67 and transferred to a secondary hospital for his rehabilitation.Conclusion:Hypocoagulopathy may be devoted to different reasons other than sepsis in extensive burns.Early recognition of the cause for coagulation disturbance is critical to make appropriate treatment and save patients’lives.This case illustrated the importance of unveiling the mist cause for coagulation disturbance occurred in extensive burn patient,which paved the way for optimal life-saving treatments.And we also recommend burn surgeons to be alerted to antibiotic-induced vitamin K deficiency-related coagulopathy among critical burn patients.展开更多
Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in...Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in the joint cavity.A recent study shows that tropoelastin(TE)regulates adhesion,proliferation and phenotypic maintenance of MSCs as a soluble additive,indicating that TE could promote MSCs-homing in regenerative medicine.In this study,we used TE as injection medium,and compared it with classic media in MSCs intra-articular injection such as normal saline(NS),hyaluronic acid(HA),and platelet-rich plasma(PRP).We found that TE could effectively improve adhesion,migration,chondrogenic differentiation of infrapatellar fat pad MSCs(IPFP-MSCs)and enhance matrix synthesis of osteoarthritic chondrocytes(OACs)in indirect-coculture system.Moreover,TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1,ERK1/2 and vinculin(VCL)in vitro.In addition,intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues.Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo.In conclusion,TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway.Our findings may provide new insights in MSCs intra-articular injection for OA treatment.展开更多
Wearable sweat sensors are gaining significant attention due to their unparalleled potential for noninvasive health monitoring.Sweat,as a kind of body fluid,contains informative physiological indicators that are relat...Wearable sweat sensors are gaining significant attention due to their unparalleled potential for noninvasive health monitoring.Sweat,as a kind of body fluid,contains informative physiological indicators that are related to personalized health status.Advances in wearable sweat sampling and routing technologies,flexible,and stretchable materials,and wireless digital technologies have led to the development of integrated sweat sensors that are comfortable,flexible,light,and intelligent.Herein,we report a flexible and integrated wearable device via incorporating a microfluidic system and a sensing chip with skin-integrated electronic format toward in-situ monitoring of uric acid(UA)in sweat that associates with gout,cardiovascular,and renal diseases.The microfluidic system validly realizes the real-time capture perspiration from human skin.The obtained detection range is 5-200μM and the detection limit is 1.79μM,which offers an importance diagnostic method for clinical relevant lab test.The soft and flexible features of the constructed device allows it to be mounted onto nearly anywhere on the body.We tested the sweat UA in diverse subjects and various body locations during exercise,and similar trends were also observed by using a commercial UA assay kit.展开更多
基金Baoding Science and Technology Bureau Project“Study on the Clinical Effects of Neurology Nursing in the Rehabilitation Process of Stroke Patients”(Project No.2441ZF275)。
文摘Objective:To explore the clinical effects of neurology nursing during the rehabilitation process of stroke patients.Methods:A total of 80 inpatients with stroke,admitted between July 2023 and July 2024,were selected for the study.They were randomly divided into two groups of 40 patients each.The control group received conventional nursing care,while the study group was provided with neurology nursing.The nursing outcomes of the two groups were compared in terms of:(1)rehabilitation progress,(2)nursing satisfaction,(3)psychological status,and(4)self-care ability.Results:The nursing efficacy and satisfaction rates in the study group were significantly higher than those in the control group(P<0.05).Pre-intervention psychological scores showed no significant differences between the two groups(P>0.05).Post-intervention,negative emotions in both groups were alleviated,with the study group showing greater improvement compared to the control group(P<0.05).Additionally,the self-care ability scores of the study group were significantly higher than those of the control group(P<0.05).Conclusion:Neurology nursing during the rehabilitation process of stroke patients demonstrated ideal clinical effects,positively contributing to patient recovery,alleviating negative psychological states,and proving beneficial for promotion.
基金supported by the Shenzhen Municipal Government of China(No.JCYJ20180507184647104)the Natural Science Foundation of Guangdong Province(Nos.2021A1515011426,2023A1515012585)。
文摘Background:Lung cancer remains the primary cause of mortality worldwide.Methylation modifications of eukaryotic messenger RNA(mRNA),recognized as one of the most prevalent chemical alterations,significantly impact the stability,splicing,and translation of mRNA.Methyltransferase-like 21A(METTL21A)functions as a non-histone methyltransferase.The role of methylation-related compounds in the development of cancer has garnered increasing interest in recent years.Methods:The expression levels of METTL21A were assessed in 86 lung cancer samples and 78 adjacent non-cancerous tissues from Taizhou Hospital.Gene expression data were sourced from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases.Multi-omics studies were conducted to examine the biological role of METTL21A within lung cancer.We thoroughly explored the potential functions and prognostic implications of METTL21A in this context.Results:An elevated expression of METTL21A was observed in lung cancer tissues.Furthermore,high levels of METTL21A expression correlate with various factors,including age,sex,race,tumor protein P53(TP53)mutations,cancer type,metastasis,and the pathological stage of lung cancer patients,indicating a relationship with poor prognosis.Additionally,METTL21A may affect lung cancer patient outcomes through distinct patterns of immune cell infiltration.Conclusion:METTL21A emerges as a promising candidate prognostic biomarker linked to immune invasion in lung cancer.
文摘Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) share a common route of transmission so that about one third of HIV infected individuals show HCV coinfection. Highly active antiretroviral therapy has offered a longer and better life to infected patients. While has removed AIDS-related diseases from the list of most common causes of death their place has been taken by complications of HCV infection, such as cirrhosis, end stage liver disease and hepatocellular carcinoma (HCC). HIV/HCV co-infection requires complex management, especially when HCC is present. Co-infected patients with HCC undergo the same therapeutic protocol as their mono-infected counterparts, but special issues such as interaction between regimens, withdrawal of therapy and choice of immunosuppressive agents, demand a careful approach by specialists. All these issues are analyzed in this minireview.
文摘Hepatocellular carcinoma(HCC)is the most frequent primary liver malignancy and the third cause of cancer-related death in the Western Countries.The well-established causes of HCC are chronic liver infections such as hepatitis B virus or chronic hepatitis C virus,nonalcoholic fatty liver disease,consumption of aflatoxins and tobacco smocking.Clinical presentation varies widely;patients can be asymptomatic while symptomatology extends from right upper abdominal quadrant paint and weight loss to obstructive jaundice and lethargy.Imaging is the first key and one of the most important aspects at all stages of diagnosis,therapy and follow-up of patients with HCC.The Barcelona Clinic Liver Cancer Staging System remains the most widely classification system used for HCC management guidelines.Up until now,HCC remains a challenge to early diagnose,and treat effectively;treating management is focused on hepatic resection,orthotopic liver transplantation,ablative therapies,chemoembolization and systemic therapies with cytotocix drugs,and targeted agents.This review article describes the current evidence on epidemiology,symptomatology,diagnosis and treatment of hepatocellular carcinoma.
文摘The liver is a solid organ with a wide variety of primary benign or malignant tumors as well as metastatic lesions.Surgical resection of these tumors remains the only curative modality.Several limitations,however,do not allow the performance of these operations.This review evaluates the indications and limitations regarding these extended hepatic resections,as well as describing all the manipulations that increase the candidates for such operations.A thorough review of the literature was performed in order to define indications for extended hepatectomy,as well as to present all methods that contribute to increasing the volume of the future remnant liver.The role of portal vein ligation,portal vein embolization,two-stage hepatectomy,and in situ liver transection are evaluated in the setting of indications and results.Extended hepatectomies are a necessity due to oncological reasons.All methods developed in order to increase the volume of the remnant liver are safe and efficient.in situ liver transection is a novel and revolutionary two-step procedure for extended hepatic resections.Further clinical studies are required to estimate long-term results and the oncological basis of this technique.
基金the National Natural Science Foundation of China,No.81772839and the Health and Family Planning Commission Foundation of Hubei Province,No.WJ2019H194.
文摘BACKGROUND Circulating tumor cells(CTCs)and survivin are indicators for tumor stage and metastasis,as well as epitheliomesenchymal transition,in various cancers,including hepatocellular cancer(HCC).AIM To explore the potential of survivin-positive CTCs,specifically,as a marker for tumor progression in HCC patients.METHODS We examined the survivin expression pattern in CTCs obtained from 179 HCC patients,and investigated the in vitro effects of survivin silencing and overexpression on the proliferation and invasion of HCC cells.CTC count and survivin expression in patient samples were examined using RNA in situ hybridization.RESULTS All 179 patients were positive for CTC markers,and 94.41%of the CTCs were positive for survivin.The CTC and survivin-positive CTC counts were significantly higher in the HCC patients than in the normal controls,and were significantly associated with tumor stage and degree of differentiation.Further,survivin overexpression was found to induce HepG2 cell proliferation,reduce apoptosis,and improve invasive ability.CONCLUSION Survivin shows upregulated expression(indicative of anti-apoptotic effects)in HCC.Thus,survivin-positive CTCs are promising as a predictor of HCC prognosis and metastasis,and their accurate measurement may be useful for the management of this cancer.
文摘Sickle cell disease(SCD) is a life-threatening genetic disorder characterized by chronic hemolytic anemia, vascular injury and multiorgan dysfunctions. Over the last few decades, there have been significant improvements in SCD management in Western countries, especially in pediatric population. An early onset of prophylaxis with Penicillin and a proper treatment of the infections have increased the overall survival in childhood. Nevertheless,management of painful episodes and prevention of organ damage are still challenging and more efforts are needed to better understand the mechanisms behind the development of chronic organ damages. Hydroxyurea(Hydroxycarbamide, HU), the only medication approved as a disease-modifying agent by the United States Food and Drug Administration and the European Medicines Agency,is usually under-used, especially in developing countries.Currently, hematopoietic stem-cell transplantation is considered the only curative option, although its use is limited by lack of donors and transplant-related toxicity.SCD symptoms are similar in children and adults, but complications and systemic organ damages increase with age, leading to early mortality worldwide. Experts in comprehensive care of young patients with SCD, especially those approaching the transition age to adulthood,are missing, leading people to rely on urgent care,increasing health care utilization costs and inappropriate treatments. It would be important to establish programs of comprehensive healthcare for patients with SCD from birth to adulthood, to improve their quality and expectancy of life.
文摘The current study was conducted to determine the epidemiology and antibiotic sensitivity pattern of bacteria isolated from blood of septicemic patients for improved antibiotic therapy. A three-year descriptive study was done at Microbiology Laboratory, Ekiti State University Teaching Hospital, Ado Ekiti, from April 2012 to April 2015. Information compiled from patients’ records includes age, sex, isolated organisms and antibiotic susceptibility patterns. Three hundred and thirteen blood cultures were collected from neonatology and pediatrics wards, Out Patients’ Department (OPD) and from other adult patients. Forty one culture plates yielded mono microbial growth (no polymicrobial growth), giving an incidence of 13.1% positive blood culture (N = 41/313). There were 58.4% Gram negative bacilli and 41.6% Gram positive cocci in the microbial growth. Bacteria isolated were Staphylococcus aureus 34% (14/41), Klebsiella species 22% (9/41), Enterococci 17% (7/41), Proteus species 12% (5/41), Escherichia coli 7% (3/41) and Streptococcal pneumoniae 7% (3/41). There was a (35%) higher occurrence of septicemia in neonates than in any other age groups in the hospital. Bacterial sensitivity to 13 antibiotic agents was determined by antibiotics disc diffusion using modified Kirby Bauer’s method. Gram-positive organisms showed a higher antibiotic sensitivity ranging from 14% - 100% than the Gram-negative bacteria (11% - 80%). Staphylococcus aureus and Klebsiella species are the most prevalent organisms. The third generation Cephalosporins (Ceftriaxone) and Floroquinolone (Levofloxacin, Ofloxacin) have proved reliable for management of these blood infections.
文摘Aim: We demonstrated the risk of developing islet autoantibodies-Insulin Autoanti-bodies (IAAs) and Islets cell Autoantibodies (ICAs)-in type-1 diabetic relatives and newly diagnosed type-1 patients compared to non-diabetic controls. We also aimed to determine the predictive strengths of both autoantibodies in the development of type-1 diabetes mellitus, and which of the two autoantibodies is a better predictive marker of type-1 diabetes mellitus among Nigerian adults. Methodology: A total number of four hundred and fifty five (455) subjects (211 (46%) males, and 244 (54%) females) aged between 35 - 76 years were recruited for the study. IAA and ICA levels were estimated using ELISA reagents from Biomerica Inc. Other parameters such as fasting blood sugar, urine glucose, and urine protein were assessed using standard biochemical techniques. Results: Relatives of type-1 diabetic patients and newly diagnosed type-1 diabetic patients were at greater risk (p < 0.05) of testing positive for more than one autoantibody (ICA and IAA) compared to non-diabetic controls. In addition, IAAs appeared to be better predictors or markers of type-1 diabetes mellitus compared to ICAs. Conclusion: The present study indicated a greater risk of autoim-mune destruction of the insulin producing beta cells of the pancrease of the type-1 relatives and newly diagnosed type-1 patients and suggests the need for periodic re-cruitment of individuals in the general population, siblings and relatives of type-1 diabetic patients for planned intervention trials. In addition, IAAs appeared to be better autoimmune markers of type-1 diabetes compared to ICAs.
文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2,broke out in December 2019 in Wuhan city of China and spread rapidly worldwide.Therefore,by March 2020,the World Health Organization declared the disease a global pandemic.Apart from the respiratory system,various other organs of the human body are also seriously affected by the virus.Liver injury in patients with a severe form of COVID-19 is estimated to be 14.8%-53.0%.Elevated levels of total bilirubin,aspartate aminotransferase and alanine aminotransferase and low levels of serum albumin and prealbumin are the main laboratory findings.Patients with pre-existing chronic liver disease and cirrhosis are much more prone to develop severe liver injury.This literature review presented the recent scientific findings regarding the pathophysiological mechanisms responsible for liver injury in critically ill patients with COVID-19,the various interactions between drugs used to treat the disease and the function of the liver and the specific tests providing the possibility of early diagnosis of severe liver injury in these patients.Moreover,it highlighted the burden that COVID-19 put on health systems worldwide and its effect on transplant programs and the care provided to critically ill patients in general and particularly to those with chronic liver disease.
文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has emerged as a major threat to global public health.The virus causes the clinical syndrome known as coronavirus disease 2019(COVID-19),in which multiple organs can get affected.Apart from manifestations of the respiratory system,which predominate,its clinical presentation is frequently accompanied by symptoms of the gastrointestinal(GI)tract and liver abnormalities.The correlation of symptoms and abnormalities with disease severity is discussed,leading to ambiguous results from international literature.Moreover,the disease infects patients with coexisting liver and GI disorders affecting both their health status and the availability of healthcare services provided to them.The risk of transmission of the disease during aerosol-generating procedures has changed the diagnostic approach and follow-up algorithms for liver and GI diseases.For the safety of both doctors and patients,telemedicine and distant evaluation have become everyday practice,whereas several routines and emergency visits at outpatient and emergency departments have been postponed or delayed.Vaccination against SARS-CoV-2 is underway,providing hope to humanity and the expectation that the post-COVID-19 era is near.This review aims to update knowledge about the manifestations of COVID-19 related to liver and GI diseases and the effect of the pandemic on the diagnostic and therapeutic procedures for these diseases with a special focus on how current practices have changed and what changes will possibly remain in the future.
基金CNPq (Conselho Nacional de De- senvolvimento Científico e Tecnologico)
文摘We aimed to study the effect of elapsed time from AML diagnosis to treatment (TDT) on OS in a group of patients from public Hospital in Brazil. 41 AML (23 M, 18 F, 41 yrs, 18 - 84 yrs, from 2001 to 2004). There were 38 de novo AML and 3 secondary, median TDT was 6 days (range 1 - 82 d);the young ones?were treated earlier than old ones (TDT 4 days vs 11, p = 0.07). Longer TDT (>10 d) was associated with worse CR rates (p = 0.02) and OS (p = 0.04). When patients were categorized into TDT from 1 - 4 d (I) vs >5 (II), those from I presented better OS than II (p = 0.004). When TDT was longer than 7 days OS decreased even more. Hb was higher in patients with TDT I vs II (8.3 vs 7.5 g/dL, p = 0.03) but WBC (p = 0.34) and platelet count (p = 0.75) were not different. Patients with TDT of 10 d were younger than TDT > 10 d (median age 41 vs 70 yrs, p = 0.001). The OS was 15.1% in 2 yrs and 8.6% in 7 yrs. Our data suggest longer TDT, when analyzed continuously, predicted for lower CR rates and OS rates.
基金supported by the grant providedby CNPq(Conselho Nacional de Desenvolvimento Científico e Tecnológico).The fellowship grant supported by CNPq was Luís Arthur Flores Pelloso,process number 140232/2001-0,period 03/01/2001 to 02/28/2005.
文摘Acute Myeloid Leukemia (AML) is a group of genetically diverse hematopoietic malignancies arising from cell progenitors developing in the myeloid pathway or from primitive stem cells. Genetic susceptibility of AML may account for an increased risk of AML due to partial metabolism of or biocativation of carcinogens. Chemical compounds are metabolized by a two-tiered phase detoxifying system. Polymorphisms in these pathways may lead to DNA damage and development of AML. We determined the frequencies of carcinogen metabolism gene polymorphisms (CYP1A1, del{GSTM1} and del{GSTT1}) in a case control-study based on polymorphism analysis. Fifty-eight consecutively AML patients (median age 62 years) and 174 sex and age-matched control group were assessed by a PCR-RFLP assay. There were 51 de novo and 7 secondary AML. CYP1A1*2A and CYP1A1*2C polymorphisms were more frequent in CG than AML p 0.001 and in contrast, CYP1A1*3 and CYP1A1*4 were more frequent in AML than CG p 0.001. There were no differences in del{GSTM1} neither del{GSTT1} between AML and CG (p = 0.999 and p = 0.539). Odds ratio for AML in patients harboring CYP1A1*3 was 2.36 (95% CI 1.2 - 4.5), 2.38 for CYP1A1*4 (95% CI 0.8 - 6.8). Adjusted OR was 2.63 for CYP1A1*3 (95% CI 1.4 - 5.1) and 2.66 for CYP1A1*4 (95% CI 0.9 - 7.8). In the multivariate analysis CYP1A1*3 polymorphism was a risk factor for AML with an OR for 3.99 (95%CI 1.9 - 8.6). To the best of our knowledge this is the first study to show that CYP1A1*3 heterozygous genotypes increase the risk of AML. Our data support that inherited absence of this carcinogen detoxification pathway may be an important determinant of AML.
文摘Granulocytic or myeloid sarcoma (MS) is a rare neoplastic condition consisting of a tumor mass of myeloid blasts with or without maturation occurring at an anatomical site other than the bone marrow the association between tuberculosis and MS is extremely rare. A 21-year-old female patient presented cough, sore throat and a suppurative swollen gum for 10 days prior to hospital admission. Physical examination revealed moderate pallor and swollen inferior gum. CBC revealed Hb6.5 g/dL, hematocrit 18.4% MCV 97 fL MCH 34 pg, WBC 18.5 ′ 109/μL (1 My/3 Bt/69 Sg/1 Eo/0 Ba/20 Ly/6 Mo), Platelets 43 ′ 109/μL. The peripheral blood smear presented with 3% blast cells (type 1) and granulocytic dysplasia. Bone marrow biopsy showed 100% cellularity. 50% of cells were from granulocytic precursors, diagnosis of granulocytic sarcoma. The diagnosis of AML was established: granulocytic sarcoma with massive gum infiltration (immature granulocytic cells) and 10% of blasts in bone marrow. The patient received induction chemotherapy (3 + 7 daunorubicin 90 mg/m2), and gum tissue culture was positive for Mycobacterium tuberculosis. Simultaneously, a qRT- PCR test confirmed the same bacteria in the gum tissue. Patient treated with isoniazid, rifampicin, pyrazinamide and ethambutol ciprofloxacin and amikacin). Remission was achieved and the patient was submitted for consolidation/ intensification (HiDAC x3) schema and referred to allogeneic HSCT. After induction and full hematological recovery there was no further evidence or recurrence of fever and lytic lesions. Currently patient is under CR and ling follow up (48 months) did not show recurrence of either AML or tuberculosis.
基金supported by the National Natural Science Foundation of China(Nos.82300561,82270523,and 82305006)the Natural Science Foundation of Chongqing(No.CSTB2023NSCQ-MSX0713)+5 种基金the Chongqing Medical Young Talents Program(No.YXQN202452 and No.YXQN202415)the High-Level Military Technological Innovation Talent Program,the Chongqing Talent Program:Innovative Leading Talents(No.CSTC2024YCJH-BGZXM0011)the Sprint Program of Joint Medical Research of Chongqing Science and Chongqing Health Commission(No.2025CCXM-001)the Youth Program of Joint Medical Research of Chongqing Science and Chongqing Health Commission(No.2025QNXM038)the Special Funding for Postdoctoral Research Projects in Chongqing,the Science Foundation of State Key Laboratory of Trauma and Chemical Poisoning(No.2024K003)the Chongqing Natural Science Foundation Innovation and Development Joint Fund(Chongqing Education Commission)(No.CSTB2024NSCQLZX0079).
文摘Ischemic/hypoxic injury significantly damages vascular function,detrimentally impacting patient outcomes.Changes in mitochondrial structure and function are closely associated with ischemia/hypoxia-induced vascular dysfunction.The mechanism of this process remains elusive.Using rat models of ischemia and hypoxic vascular smooth muscle cells(VSMCs),we combined transmission electron microscopy,super-resolution microscopy,and metabolic analysis to analyze the structure and function change of mitochondrial cristae.Multi-omics approaches revealed arginase 1(Arg1)upregulation in ischemic VSMCs,confirmed by in vivo and in vitro knockout models showing Arg1's protective effects on mitochondrial cristae,mitochondrial and vascular function,and limited the release of mtDNA.Mechanistically,Arg1 interacting with Mic1o led to mitochondrial cristae remodeling,together with hypoxia-induced VDAC1 lactylation resulting in the opening of MPTP and release of mtDNA of VSMCs.The released mtDNA led to PANoptosis of VSMCs via activation of the cGAS-STING pathway.ChIP-qPCR results demonstrated that lactatemediated Arg1 up-regulation was due to H3K18la upregulation.VSMCs targeted nano-material PLGA-PEl-siRNA@PM-a-SMA(NPsiArg1)significantly improved vascular dysfunction.This study uncovers a new mechanism of vascular dysfunction following ischemic/hypoxic injury:a damaging positive feedback loop mediated by lactate-regulated Arg1 expression between the nucleus and mitochondria,leading to mitochondria cristae disorder and mtDNA release,culminating in VSMCs PANoptosis.Targeting VSMCs Arg1 inhibition offers a potential therapeutic strategy to alleviate ischemia/hypoxia-induced vascular impairments.
基金partially funded by the Dotazione Ordinaria Ricerca(DOR)-University of Padua.
文摘Absolute erythrocytosis,due to an increased production of red blood cells,becomes manifest with hemoglobin(Hb)levels above 165 or 160 g/L or with a hematocrit(HCT)above 51%and 48%in males and females,respectively.1 A minority of patients have polycythemia vera(PV);acquired secondary erythrocytosis frequently occurs due to appropriate or inappropriate erythropoietin(EPO)excess,while congenital secondary erythrocytosis may derive from genetic defects causing tissue hypoxia.Hereditary erythrocytosis occurs in patients with genetic mutations of the oxygen-sensing pathway(VHL,PHD2,_HIF-1-alpha)or the erythropoietin receptor(EPOR)genes.2 Recently,HFE gene variants have been described in sporadic erythrocytoses.3 In a high proportion(about 70%)of patients with erythrocytosis,a specific etiology remains elusive despite extensive testing,and the diagnosis of idiopathic erythrocytosis(IE)is ruled out.
基金financially supported by the National Natural Science Foundation of China(U23A20292,62201117,62204030,and 22166016)the National Key R&D Program of China(2021YFB3201302)+1 种基金the Fundamental Research Funds for the Central Universities(DUT21RC(3)054 and DUT21RC(3)020)the Hainan Province Science and Technology Special Fund(ZDYF2022SHFZ094)。
文摘In this paper,a sensor using carbon nanospheres modified boron-doped diamond(CNs/BDD)electrode for detecting heavy metal ions rapidly in aqueous solution is studied.The CNs/BDD electrode is characterized systematically by techniques including scanning electron microscopy(SEM),X-ray diffractometer(XRD)and X-ray photoelectron spectroscopy(XPS).The electrochemical properties of the CNs/BDD electrode,BDD electrode and Au electrode are compared by cyclic voltammetry.It is found that the CNs/BDD electrode has more negative hydrogen evolution potential,a wider potential window and a larger eff ective electrode area,which is conducive to the accurate detection of heavy metal ions.The square-wave anodic stripping voltammetry(SWASV)is applied to assess and quantify the sensing capability of the CNs/BDD electrode.The eff ects of deposition potential,deposition time and other experimental parameters on the detection performance are studied.Under the optimal experimental conditions,CNs/BDD electrodes exhibit remarkable detection capabilities for lead Pb(Ⅱ)ions,in which a linear relationship within the concentration range from 1 to 140μg/L with a 0.9994 correlation coeffi cient is obtained.Meanwhile,it has excellent repeatability with a low relative standard deviation(RSD)of 0.57%,and the achieved actual limit of detection(LOD)reaches 0.01μg/L.In addition,the interference experiment results show that except for Cd(Ⅱ)ions and Cu(Ⅱ)ions,other common ions in water have little interference to Pb(Ⅱ)ion detection.The Pb(Ⅱ)ions recovery rates are 89.1–115.7%in the real water samples.Experimental analysis indicates that the CNs/BDD electrode possesses the advantages of low detection limit,short detection time,and simple experimental equipment,which meets the requirements of Pb(Ⅱ)ions fi eld detection.
文摘Background:Patients with extensive burns usually develop pro-coagulation soon after the injury if there is no sepsis occurred.We describe the case of an extensive burn adult suffering from hypocoagulation not related to sepsis,but secondary to antibiotic treatment.Case presentation:Here,we report a case of an adult male patient suffering from flame burns of 45%total body surface area(40%full thickness)combined with inhalation injury.Hypocoagulopathy with soaring prolonged activated partial thromboplastin time value occurred on third week post-burn while systemic infection had been under control by application of broad-spectrum antibiotics.Investigations showed that not the infection but vitamin K-related coagulation factor deficiency were responsible for unexpected bleeding.However,supplemental vitamin K was not the key as we expected,which prompted us trying to decode the underlying cause of coagulation disturbance in this patient and pick out the most effective treatment for live-saving.After the withdrawal of highly suspected broad-spectrum antibiotic,Meropenem^(■),disturbed vitamin K related coagulation factors gradually restored to their optimal levels so as to maintain normal coagulation status.Therefore,surgical procedures without further risk of bleeding could be carried out in time for wound recovery.The patient was discharged on post-burn day 67 and transferred to a secondary hospital for his rehabilitation.Conclusion:Hypocoagulopathy may be devoted to different reasons other than sepsis in extensive burns.Early recognition of the cause for coagulation disturbance is critical to make appropriate treatment and save patients’lives.This case illustrated the importance of unveiling the mist cause for coagulation disturbance occurred in extensive burn patient,which paved the way for optimal life-saving treatments.And we also recommend burn surgeons to be alerted to antibiotic-induced vitamin K deficiency-related coagulopathy among critical burn patients.
基金supported by CHONGQING TALENTS PROJECT(4246ZJ1)Science and technology projects of Chongqing Education Commission(KJQN202000427).
文摘Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in the joint cavity.A recent study shows that tropoelastin(TE)regulates adhesion,proliferation and phenotypic maintenance of MSCs as a soluble additive,indicating that TE could promote MSCs-homing in regenerative medicine.In this study,we used TE as injection medium,and compared it with classic media in MSCs intra-articular injection such as normal saline(NS),hyaluronic acid(HA),and platelet-rich plasma(PRP).We found that TE could effectively improve adhesion,migration,chondrogenic differentiation of infrapatellar fat pad MSCs(IPFP-MSCs)and enhance matrix synthesis of osteoarthritic chondrocytes(OACs)in indirect-coculture system.Moreover,TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1,ERK1/2 and vinculin(VCL)in vitro.In addition,intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues.Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo.In conclusion,TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway.Our findings may provide new insights in MSCs intra-articular injection for OA treatment.
基金This work was also sponsored by InnoHK Project on Project 2.2-artificial intelligent(Al)-based 3D ultrasound imaging algorithm at Hong Kong Centre for Cerebro-Cardiovascular Health Engineering(CoCHE),Center of Flexible Electronics Technology,and Qiantang Science and Technology Innovation Center.
文摘Wearable sweat sensors are gaining significant attention due to their unparalleled potential for noninvasive health monitoring.Sweat,as a kind of body fluid,contains informative physiological indicators that are related to personalized health status.Advances in wearable sweat sampling and routing technologies,flexible,and stretchable materials,and wireless digital technologies have led to the development of integrated sweat sensors that are comfortable,flexible,light,and intelligent.Herein,we report a flexible and integrated wearable device via incorporating a microfluidic system and a sensing chip with skin-integrated electronic format toward in-situ monitoring of uric acid(UA)in sweat that associates with gout,cardiovascular,and renal diseases.The microfluidic system validly realizes the real-time capture perspiration from human skin.The obtained detection range is 5-200μM and the detection limit is 1.79μM,which offers an importance diagnostic method for clinical relevant lab test.The soft and flexible features of the constructed device allows it to be mounted onto nearly anywhere on the body.We tested the sweat UA in diverse subjects and various body locations during exercise,and similar trends were also observed by using a commercial UA assay kit.
