Since X-linked chronic granulomatosis disease(X-CGD)exhibits no specific clinical symptoms at an early stage,early diagnosis is difficult and depends predominantly on neonatal screening.Therefore,the aim of this study...Since X-linked chronic granulomatosis disease(X-CGD)exhibits no specific clinical symptoms at an early stage,early diagnosis is difficult and depends predominantly on neonatal screening.Therefore,the aim of this study was to explore routine biomarkers for X-CGD in children and provide clues for early diagnosis.The cases of 10 children with X-CGD diagnosed at Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology from 2013 to 2016 and 122 Chinese children with X-CGD reported in the literature were summarized.Serum biomarkers and clinical symptoms at acute infection were organized.A total of 132 children with X-CGD were enrolled in this study.For 55.8%of the patients,the diagnosis was delayed more than one year after the onset of the first symptoms because no typical clinical symptoms manifested.Children with X-CGD at an acute infection stage showed three recurrent signs in temis of serum biomarkers:(1)the total number of white blood cells(especially N%)was in creased significantly,accompanied by anemia in some cases;(2)C-reactive protein(CRP)levels were increased significantly;and(3)most of the patients exhibited very high serum IgG levels(>12 g/L).Diagnosis of X-CGD at an early age is difficult because of its nonspecific clinical features.Our study suggested children with X-CGD suffering acute infection show increases in three typical serum biomarkers,which can provide clues for early diagnosis.展开更多
Neuronal and glial differentiation potential of skin-derived precursors is of great interest for clinical application in the treatment of neurodegenerative disease. In this sense, the pig model is a great candidate fo...Neuronal and glial differentiation potential of skin-derived precursors is of great interest for clinical application in the treatment of neurodegenerative disease. In this sense, the pig model is a great candidate for the development of preclinical models. To the date, skin-derived precursor spheres have not been isolated from adult porcine skin. In order to optimize the protocol for isolating dermal precursor spheres from adult porcine skin, 15 porcine skin biopsies were subjected to three different processing protocols. Liberase-based digestion of ventral porcine skin gave rise to more cells with spherogenic capacity than other protocols and these spheres presented phenotypic and differentiation potential consistent with bona fide skin-derived precursor cells.展开更多
Introduction1Vitrification is an effective method for cryopreservation of cell s[1.2].Hbwever,cells are usually damageddue to the osmotic injuy caused by the higher concentra-tions of cryoprotective agents(CPA)during ...Introduction1Vitrification is an effective method for cryopreservation of cell s[1.2].Hbwever,cells are usually damageddue to the osmotic injuy caused by the higher concentra-tions of cryoprotective agents(CPA)during CPA remov-ing.The ice recrystallization in thawing solution can alsohurt cells serio usly.Antifreeze glycoprotein(AFGPs)isextremely efficient at inhibiting ice recrystallization[3].展开更多
Decellularized organs and tissues are emerging within the field ofregenerative medicine to meet the growing demand for organand tissue transplantation. Quality control of these acellular matrices prior to transplantat...Decellularized organs and tissues are emerging within the field ofregenerative medicine to meet the growing demand for organand tissue transplantation. Quality control of these acellular matrices prior to transplantation is of paramount importance to ensure the absence of an adverse reaction. In particular, thoroughevaluation of the DNA content is essential but also poses technical challenges. Therefore, in this study, we compared differentmethods for quantitative and qualitative evaluation of DNA content in native and decellularized skeletal muscle tissue to identifystrengths and weaknesses for each. Histological analysis revealedthat Feulgen staining is more sensitive and robust than the commonly used hematoxylin–eosin and 40,6-diamidino-2-phenylindole staining for detection of remaining nuclear material.Furthermore, gel electrophoresis allowed to identify the quality and length of remaining DNA fragments. The results of the quantitativeanalysis indicated that direct measurement of DNA content in tissue lysates is preferred over silica-based extraction methods, since thelatter resulted in the loss of small DNA fragments during extraction. Moreover, a weight loss correction factor should be implementedto take into account the impact of the decellularization on the extracellular matrix. With regard to the detection method, the resultsrevealed that a fluorescence-based approach is more accurate than the use of UV/VIS absorbance. Through combination of the proposedmethods, it should be possible to achieve a more standardized evaluation of novel acellular matrices in terms of DNA content and toenhance the predictability of clinical success.展开更多
In this study, natural materials （sodium alginate, dextran, gelatin and carboxymethyl chitosan） were modified to get aldehyde components and amino components. Upon mixing the two-component solutions together, four k...In this study, natural materials （sodium alginate, dextran, gelatin and carboxymethyl chitosan） were modified to get aldehyde components and amino components. Upon mixing the two-component solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and could seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution confirmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of commercial fibrin glue and the blank control. The histological staining observation by hematoxylin and eosin staining （HE） and Masson＇s trichrome staining （MTC） methods suggested that the hydrogels had good biocompatibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and could improve the formation of new collagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive.展开更多
Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate fo...Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate for generating tissue engineering scaffolds. Electrospinning is a simple and effective method to fabricate nanofibers, which has several amazing characteristics such as very large surface area to volume ratio, flexibility in surface functionalities, and superior mechanical performance. The electro- spun nanofibers from SF and its blends have been used for varied tissue engineering. This paper will give a brief review about the structure, properties and applications of SF and blend nanofibers via electrospinning.展开更多
Globally, cancer is growing at an alarming pace, which calls for development of more efficient cancer treatments. Conventional chemotherapy and radiotherapy have become crucial first-line clinical treatments for cance...Globally, cancer is growing at an alarming pace, which calls for development of more efficient cancer treatments. Conventional chemotherapy and radiotherapy have become crucial first-line clinical treatments for cancer. However, along with their wide usage, limited therapeutic effects, severe adverse reactions, unaffordable costs, and complicated operations lead to failures of these treat- ments. Moreover, the emergence of multidrug resistance inhibits the longtirne usage of chemotherapeutics. One of the major causes of treatment failure is the insufficient sensitivity of cancer cells to therapeutic drugs or treatments. With the rigorous development of nanotechnolog~ tailored nanoparticles can efficiently sensitize malignant cells by inducing intracellular structural and functional changes, which could affect vital intracellular processes such as metabolism, signal conduction, proliferation, cell death as well as intracellular drug delivery. Here, we review recent advances in nanomaterial-assisted sensitization of oncotherapy, and challenges and strategies in the development of nanomedical approaches.展开更多
Gold nanoparticles （Au NPs） have been widely utilized in biomedical appli- cations owing to their attractive features and biocompatibility, which greatly increase the risk of humans＂ being exposed to Au NPs, includ...Gold nanoparticles （Au NPs） have been widely utilized in biomedical appli- cations owing to their attractive features and biocompatibility, which greatly increase the risk of humans＂ being exposed to Au NPs, including pregnant women. In contrast to mature cells, embryos are more susceptible to outside disruptive stimuli. Nonetheless, a possible inhibitory effect of nanomaterials on embryonic development is usually ignored as long as the NPs do not have significant cytotoxic effects. According to our results, a minimal ＂nontoxic＂ concentration of Au NPs during early pregnancy can have lethal inhibitory effects on embryos in vivo and in vitro. We conducted important experiments on the influence of Au NPs on embryonic development and found that Au NPs can disturb embryonic development in a size- and concentration-dependent manner. Au NPs of 15 nm in diameter downregulated the expression pattern of distinct germ layer markers both at mRNA and protein levels; this action prevented differentiation of all three embryonic germ layers. Consequentl~ fetal resorption was observed. Our work reveals the impact of Au NPs on embryonic development and will provide an important guidance and serve as a reference for biomedical applications of Au NPs with minimal side effects.展开更多
基金Natural Science Foundation of Hubei,China(No.2017CFB645)JinLei Research Fund for Pediatric Endocrine Young Physician,China(No.PEGRF201304006)the Ministry of Science and Technology(No.2017ZX09304022).
文摘Since X-linked chronic granulomatosis disease(X-CGD)exhibits no specific clinical symptoms at an early stage,early diagnosis is difficult and depends predominantly on neonatal screening.Therefore,the aim of this study was to explore routine biomarkers for X-CGD in children and provide clues for early diagnosis.The cases of 10 children with X-CGD diagnosed at Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology from 2013 to 2016 and 122 Chinese children with X-CGD reported in the literature were summarized.Serum biomarkers and clinical symptoms at acute infection were organized.A total of 132 children with X-CGD were enrolled in this study.For 55.8%of the patients,the diagnosis was delayed more than one year after the onset of the first symptoms because no typical clinical symptoms manifested.Children with X-CGD at an acute infection stage showed three recurrent signs in temis of serum biomarkers:(1)the total number of white blood cells(especially N%)was in creased significantly,accompanied by anemia in some cases;(2)C-reactive protein(CRP)levels were increased significantly;and(3)most of the patients exhibited very high serum IgG levels(>12 g/L).Diagnosis of X-CGD at an early age is difficult because of its nonspecific clinical features.Our study suggested children with X-CGD suffering acute infection show increases in three typical serum biomarkers,which can provide clues for early diagnosis.
基金financed by grants provided by the Department of Industry,Innovation,Commerce and Tourism of the Basque Government(Gaitek 10/002)Diputacion Foral de Gipuzkoa(OF 53/2011)+1 种基金Ministerio de Ciencia e Innovacion(PI10/02871 and INNPACTO pro-grams,IPT-300000-2010-17)A.I.was supported by the“Programa I3SNS”(CES09/015)from Instituto de Salud Carlos III and by Osaki-detza(Spain).
文摘Neuronal and glial differentiation potential of skin-derived precursors is of great interest for clinical application in the treatment of neurodegenerative disease. In this sense, the pig model is a great candidate for the development of preclinical models. To the date, skin-derived precursor spheres have not been isolated from adult porcine skin. In order to optimize the protocol for isolating dermal precursor spheres from adult porcine skin, 15 porcine skin biopsies were subjected to three different processing protocols. Liberase-based digestion of ventral porcine skin gave rise to more cells with spherogenic capacity than other protocols and these spheres presented phenotypic and differentiation potential consistent with bona fide skin-derived precursor cells.
文摘Introduction1Vitrification is an effective method for cryopreservation of cell s[1.2].Hbwever,cells are usually damageddue to the osmotic injuy caused by the higher concentra-tions of cryoprotective agents(CPA)during CPA remov-ing.The ice recrystallization in thawing solution can alsohurt cells serio usly.Antifreeze glycoprotein(AFGPs)isextremely efficient at inhibiting ice recrystallization[3].
基金supported by the Research Foundation—Flanders[G0D3620N and 1133520N]。
文摘Decellularized organs and tissues are emerging within the field ofregenerative medicine to meet the growing demand for organand tissue transplantation. Quality control of these acellular matrices prior to transplantation is of paramount importance to ensure the absence of an adverse reaction. In particular, thoroughevaluation of the DNA content is essential but also poses technical challenges. Therefore, in this study, we compared differentmethods for quantitative and qualitative evaluation of DNA content in native and decellularized skeletal muscle tissue to identifystrengths and weaknesses for each. Histological analysis revealedthat Feulgen staining is more sensitive and robust than the commonly used hematoxylin–eosin and 40,6-diamidino-2-phenylindole staining for detection of remaining nuclear material.Furthermore, gel electrophoresis allowed to identify the quality and length of remaining DNA fragments. The results of the quantitativeanalysis indicated that direct measurement of DNA content in tissue lysates is preferred over silica-based extraction methods, since thelatter resulted in the loss of small DNA fragments during extraction. Moreover, a weight loss correction factor should be implementedto take into account the impact of the decellularization on the extracellular matrix. With regard to the detection method, the resultsrevealed that a fluorescence-based approach is more accurate than the use of UV/VIS absorbance. Through combination of the proposedmethods, it should be possible to achieve a more standardized evaluation of novel acellular matrices in terms of DNA content and toenhance the predictability of clinical success.
基金This research was supported by the National Key Research Program of China （2016YFA0201702 of 2016YFA0201700）, the National Natural Science Foundation of China （Grant No. 31470941）, the Science and Technology Commission of Shanghai Municipality （Nos. 15JC1490100, 15441905100）, Donghua University Graduate Student Innovation Fund Project （CUSF-DH-D-2015032）, Yantai Double Hundred Talent Plan and ＂111 Project＂ Biomedical Textile Materials Science and Technology, China （No. B07024）.
文摘In this study, natural materials （sodium alginate, dextran, gelatin and carboxymethyl chitosan） were modified to get aldehyde components and amino components. Upon mixing the two-component solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and could seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution confirmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of commercial fibrin glue and the blank control. The histological staining observation by hematoxylin and eosin staining （HE） and Masson＇s trichrome staining （MTC） methods suggested that the hydrogels had good biocompatibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and could improve the formation of new collagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive.
文摘Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate for generating tissue engineering scaffolds. Electrospinning is a simple and effective method to fabricate nanofibers, which has several amazing characteristics such as very large surface area to volume ratio, flexibility in surface functionalities, and superior mechanical performance. The electro- spun nanofibers from SF and its blends have been used for varied tissue engineering. This paper will give a brief review about the structure, properties and applications of SF and blend nanofibers via electrospinning.
文摘Globally, cancer is growing at an alarming pace, which calls for development of more efficient cancer treatments. Conventional chemotherapy and radiotherapy have become crucial first-line clinical treatments for cancer. However, along with their wide usage, limited therapeutic effects, severe adverse reactions, unaffordable costs, and complicated operations lead to failures of these treat- ments. Moreover, the emergence of multidrug resistance inhibits the longtirne usage of chemotherapeutics. One of the major causes of treatment failure is the insufficient sensitivity of cancer cells to therapeutic drugs or treatments. With the rigorous development of nanotechnolog~ tailored nanoparticles can efficiently sensitize malignant cells by inducing intracellular structural and functional changes, which could affect vital intracellular processes such as metabolism, signal conduction, proliferation, cell death as well as intracellular drug delivery. Here, we review recent advances in nanomaterial-assisted sensitization of oncotherapy, and challenges and strategies in the development of nanomedical approaches.
文摘Gold nanoparticles （Au NPs） have been widely utilized in biomedical appli- cations owing to their attractive features and biocompatibility, which greatly increase the risk of humans＂ being exposed to Au NPs, including pregnant women. In contrast to mature cells, embryos are more susceptible to outside disruptive stimuli. Nonetheless, a possible inhibitory effect of nanomaterials on embryonic development is usually ignored as long as the NPs do not have significant cytotoxic effects. According to our results, a minimal ＂nontoxic＂ concentration of Au NPs during early pregnancy can have lethal inhibitory effects on embryos in vivo and in vitro. We conducted important experiments on the influence of Au NPs on embryonic development and found that Au NPs can disturb embryonic development in a size- and concentration-dependent manner. Au NPs of 15 nm in diameter downregulated the expression pattern of distinct germ layer markers both at mRNA and protein levels; this action prevented differentiation of all three embryonic germ layers. Consequentl~ fetal resorption was observed. Our work reveals the impact of Au NPs on embryonic development and will provide an important guidance and serve as a reference for biomedical applications of Au NPs with minimal side effects.
