The present study compared eight breeds of cattle differing in gender （heifers, buls and steers） to determine associations between muscle characteristics and meat sensory qualities of theLongissimus thoracis muscle....The present study compared eight breeds of cattle differing in gender （heifers, buls and steers） to determine associations between muscle characteristics and meat sensory qualities of theLongissimus thoracis muscle. Animal types differed in al the muscle characteristics and sensory qualities. Many correlations among muscle characteristics and among sensory qualities were consistent for most animal types. Isocitrate dehydrogenase （ICDH） activities alowed discrimination of muscles with respect to myosin heavy chain （MyHC）-I proportions for al animal types. Lactate dehydrogenase （LDH） and phos-phofructokinase （PFK） activities were positively correlated for most animal types. Overal liking was correlated with beef lfavour and abnormal lfavour in al animal types and with global tenderness for al animal types except for Charolais cross breed steers. For al animal types except for Angus×Friesian heifers, beef lfavour and abnormal lfavour were negatively correlated. Overal liking was not correlated with juiciness. PFK, ICDH and citrate synthase （CS） activities were strongly associated with tenderness, beef lfavour and overal liking when average values for al animal types were used. However, associations between muscle characteristics and sensory qualities within animal types were weak and inconsistent.展开更多
Our modern society is facing major challenges such as reducing world hunger by increasing protein resources despite an increase in protein demands due to the presence of more and more human beings on the earth. Other ...Our modern society is facing major challenges such as reducing world hunger by increasing protein resources despite an increase in protein demands due to the presence of more and more human beings on the earth. Other challenges include reducing potential environmental degradation due to human activities and also due to livestock. In addition to that, some people are concerned by reducing potential discomfort of animals on modern farms or maybe avoiding killing animals to eat them.展开更多
Background:Scarce is knowledge on the process regulating the development of acid secretion,orexigenic signaling,and chemosensing in the stomach of young pigs.Changes of early microbial encounters by suckling pigs can ...Background:Scarce is knowledge on the process regulating the development of acid secretion,orexigenic signaling,and chemosensing in the stomach of young pigs.Changes of early microbial encounters by suckling pigs can interact with the gut maturation,by the induction of different molecular signaling.Our goal was to assess if the age of offspring and the maternal environment,influenced by sow antibiotic treatment peripartum,could affect gastric morphology and the expression of genes involved in the control of hydrochloric secretion,feed intake,taste,and inflammation in offspring stomach.Methods:84 pigs from sows fed a diet with amoxicillin(on-d10 to+d21 from farrowing,ANT)or without(CON)were sacrificed at d14,d21,d28(weaning)or d42.Samples of oxyntic(OXY),pyloric(PY)and cardiac mucosae close to OXY were collected and parietal and enteroendocrine cells(EECs)were counted.Relative gene expression of a set of 11 key genes(ATP4A,SSTR2,GAST,GHRL,MBOAT4,PCSK1,GNAT1,TAS1R1,TAS1R3,IL8 and TNF)was assessed by qRT-PCR.In addition,40 offspring obtained from the same ANT and CON sows were offered a normal or a fatenriched diet for 4 weeks between 140 and 169 d of age,and then OXY and PY were sampled.Results:The number of parietal and EECs increased with age(P<0.001).ATP4A increased with age(within suckling,P=0.043,post-weaning vs.suckling,P<0.001),SSTR2 increased only after weaning(P<0.001).In OXY,GHRL increased during suckling(P=0.012),and post-weaning as a trend(P=0.088).MBOAT4 tended to increase during suckling(P=0.062).TAS1R1 increased from suckling to post-weaning period(P=0.001)and was lower in ANT offspring(P=0.013).GNAT1 in PY was higher in ANT offspring(P=0.041).Antibiotic treatment of sows peripartum increased expression of GHRL and MBOAT4 in OXY of growing-finishing offspring aged 5 months.Conclusions:Data show that sensing for umami taste and ghrelin regulation can be affected by maternal environment,but the development of acid secretion,orexigenic signaling and taste perception in the stomach are mostly developmentally controlled.展开更多
The prevalence of obesity in children has increased dramatically during the past decades, and requires efficient care. Objectives. -To determine changes in anthropometric parameters and basal metabolic rate (BMR) in o...The prevalence of obesity in children has increased dramatically during the past decades, and requires efficient care. Objectives. -To determine changes in anthropometric parameters and basal metabolic rate (BMR) in obese adolescents during and after 2 types of weight-reduction programs. Subjects and methods. -Twenty-six adolescents (group I, Z-score of BMI=4.72) followed a 9-month-weight reduction program including a moderate energy restriction and regular physical activities in a specialized institution. In addition, 39 adolescents (group E, Z-score of BMI=2.83) followed at home a 9-month-weight reduction program including medical and dietetic advices. Body composition (by impedancemetry) and BMR (by indirect calorimetry) were assessed before the beginning (M0), 4 months after (M4) and at the end (M9) of the programs, then 4 months (M13) and 16 month (M25) after the end of the weight-reduction programs. Results. -Twenty-two adolescents in group I and 20 adolescents in group E completed the study. At M0, age, body weight (BW), fat-free mass (FFM)-and BMR of subjects of group I were higher (1.0 year, 36 % , 30 % and 23 % , respectively, P < 0.001), than those of group E. Pubertal stage and percentage of fat mass (FM) were not significantly different between the 2 groups. At M9, adolescents of group I showed significant reductions in BW and FM (-19 and -37 % , respectively, P< 0.001), but not significant differences in FFM. In addition, BMR decreased significantly between M0 and M4, both in absolute value (-6.7% , P< 0.001) and after adjustment for FFM (-5.8% , P< 0.001), and the difference was maintained until M9. Between M9 and M13,BW and Z-sc-ore of BMI were maintained in 12 adolescents, but increased (+ 9.7% and 14.8% , respectively, P< 0.001) in 10 adolescents. However, BMR did not change significantly in all adolescents. Between M13 and M25, BW, FM, FFM and BMR increased significantly (+ 13% , + 34% , + 6% et + 5% , respectively, P< 0.001). During the 25 month period, adolescents of group E showed significant increases in BW, FFM and BMR (+ 8% , + 14% and + 10% , respectively, P< 0.001), and maintained their Z-score and FM. Conclusion. -The reduction in BMR during the weight-reduction program at the institution could contribute to body weight regain in the post-obese adolescents if they do not maintain the lifestyle habits taught during the weight-reduction period. In other respects, Z-score was stabilized in 51% of domiciled obese adolescents.展开更多
The present report overviews a new family of bovine serpins able to inhibit pseudo-irreversibly initiator and effector caspases, a group of cysteine proteases in charge of cell dismantling during apoptosis, a finely r...The present report overviews a new family of bovine serpins able to inhibit pseudo-irreversibly initiator and effector caspases, a group of cysteine proteases in charge of cell dismantling during apoptosis, a finely regulated cell death process. The 8 members identified at the gene level showed a high homology with human SERPINA3 and were therefore designed bovSERPINA3-1 to A3-8. At least six of them are able to inhibit caspases. Two of them (bovSERPINA3-1 and A3-3) have been purified from bovine muscle and extensively investigated during these last years. After a general presentation of the serpin superfamily, the kinetic aspects of their interaction with human cas-pases 3 and 8 were studied and findings obtained suggest that caspases could be their target enzymes in living cells. In muscle and primary myoblast in culture, they showed an intracellular localization and because of their high level in blood, they can be exported. Two biological functions (potential regulator of apoptosis and expression during myoblast differentiation) were investigated and it was concluded that they are very likely a efficient regulator of apoptosis, a proposal supported by their high expression in proliferating myoblast (cell survival is essential during this differentiation phase) but not in myotubes.展开更多
In the French Alps, some grasslands have been abandoned, others are invaded by scrub whereas they are still used by farmers What are the relationships between land use changes and other changes at farm level that lead...In the French Alps, some grasslands have been abandoned, others are invaded by scrub whereas they are still used by farmers What are the relationships between land use changes and other changes at farm level that lead to scrub invasion？ We hypothesize that they are linked by two work organization processes： process of changes （changes in the household and farm） leading to less intensive land use; and annual processes （sequences of activities during the year） and their repetition from one year to another, leading to insufficient land maintenance. In this paper, we present a study carried out in the Northern French Alps, based on surveys with livestock farmers. It appears that land use changes are often the consequence of other changes made to address work problems, and land use sequences are defined according to workforce and prioritization of tasks. This highlights the importance of considering farmers as workers in order to evaluate land use changes at the farm level and to link them to the human dimension within farming systems.展开更多
基金the framework of the EU Project ProSafeBeef(FOOD-CT-2006-36241)with INRA(Institut National de la Recherche Agronomique,France)Quality Assurance number AQ284
文摘The present study compared eight breeds of cattle differing in gender （heifers, buls and steers） to determine associations between muscle characteristics and meat sensory qualities of theLongissimus thoracis muscle. Animal types differed in al the muscle characteristics and sensory qualities. Many correlations among muscle characteristics and among sensory qualities were consistent for most animal types. Isocitrate dehydrogenase （ICDH） activities alowed discrimination of muscles with respect to myosin heavy chain （MyHC）-I proportions for al animal types. Lactate dehydrogenase （LDH） and phos-phofructokinase （PFK） activities were positively correlated for most animal types. Overal liking was correlated with beef lfavour and abnormal lfavour in al animal types and with global tenderness for al animal types except for Charolais cross breed steers. For al animal types except for Angus×Friesian heifers, beef lfavour and abnormal lfavour were negatively correlated. Overal liking was not correlated with juiciness. PFK, ICDH and citrate synthase （CS） activities were strongly associated with tenderness, beef lfavour and overal liking when average values for al animal types were used. However, associations between muscle characteristics and sensory qualities within animal types were weak and inconsistent.
文摘Our modern society is facing major challenges such as reducing world hunger by increasing protein resources despite an increase in protein demands due to the presence of more and more human beings on the earth. Other challenges include reducing potential environmental degradation due to human activities and also due to livestock. In addition to that, some people are concerned by reducing potential discomfort of animals on modern farms or maybe avoiding killing animals to eat them.
基金This work was financially supported by the European Union(contract No.227549)through the Interplay project.The authors are solely responsible for the work described in this paper,and their opinions are not necessarily those of the European Union.
文摘Background:Scarce is knowledge on the process regulating the development of acid secretion,orexigenic signaling,and chemosensing in the stomach of young pigs.Changes of early microbial encounters by suckling pigs can interact with the gut maturation,by the induction of different molecular signaling.Our goal was to assess if the age of offspring and the maternal environment,influenced by sow antibiotic treatment peripartum,could affect gastric morphology and the expression of genes involved in the control of hydrochloric secretion,feed intake,taste,and inflammation in offspring stomach.Methods:84 pigs from sows fed a diet with amoxicillin(on-d10 to+d21 from farrowing,ANT)or without(CON)were sacrificed at d14,d21,d28(weaning)or d42.Samples of oxyntic(OXY),pyloric(PY)and cardiac mucosae close to OXY were collected and parietal and enteroendocrine cells(EECs)were counted.Relative gene expression of a set of 11 key genes(ATP4A,SSTR2,GAST,GHRL,MBOAT4,PCSK1,GNAT1,TAS1R1,TAS1R3,IL8 and TNF)was assessed by qRT-PCR.In addition,40 offspring obtained from the same ANT and CON sows were offered a normal or a fatenriched diet for 4 weeks between 140 and 169 d of age,and then OXY and PY were sampled.Results:The number of parietal and EECs increased with age(P<0.001).ATP4A increased with age(within suckling,P=0.043,post-weaning vs.suckling,P<0.001),SSTR2 increased only after weaning(P<0.001).In OXY,GHRL increased during suckling(P=0.012),and post-weaning as a trend(P=0.088).MBOAT4 tended to increase during suckling(P=0.062).TAS1R1 increased from suckling to post-weaning period(P=0.001)and was lower in ANT offspring(P=0.013).GNAT1 in PY was higher in ANT offspring(P=0.041).Antibiotic treatment of sows peripartum increased expression of GHRL and MBOAT4 in OXY of growing-finishing offspring aged 5 months.Conclusions:Data show that sensing for umami taste and ghrelin regulation can be affected by maternal environment,but the development of acid secretion,orexigenic signaling and taste perception in the stomach are mostly developmentally controlled.
文摘The prevalence of obesity in children has increased dramatically during the past decades, and requires efficient care. Objectives. -To determine changes in anthropometric parameters and basal metabolic rate (BMR) in obese adolescents during and after 2 types of weight-reduction programs. Subjects and methods. -Twenty-six adolescents (group I, Z-score of BMI=4.72) followed a 9-month-weight reduction program including a moderate energy restriction and regular physical activities in a specialized institution. In addition, 39 adolescents (group E, Z-score of BMI=2.83) followed at home a 9-month-weight reduction program including medical and dietetic advices. Body composition (by impedancemetry) and BMR (by indirect calorimetry) were assessed before the beginning (M0), 4 months after (M4) and at the end (M9) of the programs, then 4 months (M13) and 16 month (M25) after the end of the weight-reduction programs. Results. -Twenty-two adolescents in group I and 20 adolescents in group E completed the study. At M0, age, body weight (BW), fat-free mass (FFM)-and BMR of subjects of group I were higher (1.0 year, 36 % , 30 % and 23 % , respectively, P < 0.001), than those of group E. Pubertal stage and percentage of fat mass (FM) were not significantly different between the 2 groups. At M9, adolescents of group I showed significant reductions in BW and FM (-19 and -37 % , respectively, P< 0.001), but not significant differences in FFM. In addition, BMR decreased significantly between M0 and M4, both in absolute value (-6.7% , P< 0.001) and after adjustment for FFM (-5.8% , P< 0.001), and the difference was maintained until M9. Between M9 and M13,BW and Z-sc-ore of BMI were maintained in 12 adolescents, but increased (+ 9.7% and 14.8% , respectively, P< 0.001) in 10 adolescents. However, BMR did not change significantly in all adolescents. Between M13 and M25, BW, FM, FFM and BMR increased significantly (+ 13% , + 34% , + 6% et + 5% , respectively, P< 0.001). During the 25 month period, adolescents of group E showed significant increases in BW, FFM and BMR (+ 8% , + 14% and + 10% , respectively, P< 0.001), and maintained their Z-score and FM. Conclusion. -The reduction in BMR during the weight-reduction program at the institution could contribute to body weight regain in the post-obese adolescents if they do not maintain the lifestyle habits taught during the weight-reduction period. In other respects, Z-score was stabilized in 51% of domiciled obese adolescents.
文摘The present report overviews a new family of bovine serpins able to inhibit pseudo-irreversibly initiator and effector caspases, a group of cysteine proteases in charge of cell dismantling during apoptosis, a finely regulated cell death process. The 8 members identified at the gene level showed a high homology with human SERPINA3 and were therefore designed bovSERPINA3-1 to A3-8. At least six of them are able to inhibit caspases. Two of them (bovSERPINA3-1 and A3-3) have been purified from bovine muscle and extensively investigated during these last years. After a general presentation of the serpin superfamily, the kinetic aspects of their interaction with human cas-pases 3 and 8 were studied and findings obtained suggest that caspases could be their target enzymes in living cells. In muscle and primary myoblast in culture, they showed an intracellular localization and because of their high level in blood, they can be exported. Two biological functions (potential regulator of apoptosis and expression during myoblast differentiation) were investigated and it was concluded that they are very likely a efficient regulator of apoptosis, a proposal supported by their high expression in proliferating myoblast (cell survival is essential during this differentiation phase) but not in myotubes.
文摘In the French Alps, some grasslands have been abandoned, others are invaded by scrub whereas they are still used by farmers What are the relationships between land use changes and other changes at farm level that lead to scrub invasion？ We hypothesize that they are linked by two work organization processes： process of changes （changes in the household and farm） leading to less intensive land use; and annual processes （sequences of activities during the year） and their repetition from one year to another, leading to insufficient land maintenance. In this paper, we present a study carried out in the Northern French Alps, based on surveys with livestock farmers. It appears that land use changes are often the consequence of other changes made to address work problems, and land use sequences are defined according to workforce and prioritization of tasks. This highlights the importance of considering farmers as workers in order to evaluate land use changes at the farm level and to link them to the human dimension within farming systems.
