BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically...BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically experience a poor prognosis,highlighting an urgent need to explore novel treatment approaches.This study evaluated the overall efficacy and safety of CMT vs CMT combined with immune checkpoint inhibitors(ICIs)in the treatment of EC patients with liver metastases.Furthermore,prognostic factors influencing outcomes in this patient population were identified.AIM To evaluate the efficacy and safety of first-line chemoimmunotherapy for EC patients with liver metastases and to analyze prognostic factors.METHODS This retrospective study included 126 EC patients with liver metastases at Zhejiang Cancer Hospital between 2014 and 2024.Patients receiving CMT were compared with those receiving CMT+ICI.Analyzed variables included clinicopathological features,treatment history,characteristics of metastasis,systemic and local treatments,overall survival(OS),and treatment-related adverse events(TRAEs).Prognostic factors were evaluated using univariate and multivariate Cox proportional-hazards regression models.Finally,efficacy outcomes and TRAE profiles were compared between the two groups.RESULTS A significant difference in median OS was identified between the two groups(10.8 months in the CMT group vs 20.8 months in the CMT+ICI group,P=0.004).The CMT+ICI group also demonstrated a significantly longer median progression-free survival of 11.7 months(P<0.001).Patients receiving combination therapy exhibited significantly improved systemic objective response rate and disease control rate.Multivariate analysis identified key factors significantly influencing OS in EC patients with liver metastases:Karnofsky Performance Status score≥70,receipt of local therapy for liver metastases,and the number of cycles of CMT and immunotherapy received.Furthermore,the incidence of TRAEs did not significantly differ between the CMT+ICI and CMT groups.CONCLUSION For EC patients with liver metastases,the combination of CMT and ICIs demonstrates significantly superior efficacy compared with CMT alone,while maintaining manageable TRAEs.展开更多
This study explores the significance of using two-dimensional shear wave elastography(2D-SWE)to assess liver stiffness(LS)and spleen area(SPA)for predicting post-hepatectomy liver failure(PHLF).By providing a non-inva...This study explores the significance of using two-dimensional shear wave elastography(2D-SWE)to assess liver stiffness(LS)and spleen area(SPA)for predicting post-hepatectomy liver failure(PHLF).By providing a non-invasive method to measure LS,which correlates with the degree of liver fibrosis,and SPA,an indicator of portal hypertension,2D-SWE offers a comprehensive evaluation of a patient’s hepatic status.These advancements are particularly crucial in hepatic surgery,where accurate preoperative assessments are essential for optimizing surgical outcomes and minimizing complications.This letter highlights the prac-tical implications of integrating 2D-SWE into clinical practice,emphasizing its potential to improve patient safety and surgical precision by enhancing the ability to predict PHLF and tailor surgical approaches accordingly.展开更多
Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optic...Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optical quality,and high safety,but the risks of postoperative complications such as glaucoma and anterior subcapsular opacification still exist.Vault is an important indicator for evaluating the safety after ICL implantation,and its ideal state is crucial for preventing complications.Studies have shown that iris morphology has a significant impact on vault.In order to further optimize surgical outcomes and improve surgical safety,this review comprehensively reviews the research progress of iris-related parameters in ICL implantation and discusses the importance of various parameters in preoperative evaluation and postoperative follow-up.展开更多
BACKGROUND In recent years,the number of studies on spondylolisthesis has been increasing,and there are many publications on this disorder.To our knowledge,there is no bibliometric analysis of spondylolisthesis to dat...BACKGROUND In recent years,the number of studies on spondylolisthesis has been increasing,and there are many publications on this disorder.To our knowledge,there is no bibliometric analysis of spondylolisthesis to date.AIM To investigate emerging directions in Spondylolisthesis research and systematically evaluate the academic literature with the highest citation impact within this field.METHODS All data were collected from the Web of Science Core Collection database.Years of publications,countries,journals,institutions and total number of citations were extracted and analyzed by VOSviewer software.In addition,we analyzed the top 100 most-cited articles on spondylolisthesis.RESULTS A total of 1831 articles related to spondylolisthesis were identified.The frequency of publications on spondylolisthesis has increased dramatically over time.Among all countries,United States has contributed the most publications on spondylolisthesis(n=574).The institution with the most articles was the University of California,San Francisco(n=52).Spine topped the list of journals and has published 291 spondylolisthesis-related reports.The hotspot of research changed from posterolateral fusion to interbody fusion.CONCLUSION In recent years,academic investigations on spondylolisthesis have exhibited significant growth.As the inaugural bibliometric evaluation in this domain,our research establishes a methodological framework for synthesizing the historical progression and current advancements of spondylolisthesis studies.展开更多
Self-inflicted and interpersonal violence are the two main types of adolescent violence.Individuals involved in self-inflicted violence both cause and receive the harm,while those who engage in interpersonal violence ...Self-inflicted and interpersonal violence are the two main types of adolescent violence.Individuals involved in self-inflicted violence both cause and receive the harm,while those who engage in interpersonal violence often aim to harm others.Self-harm and suicide are common types of selfinflicted violence.Non-fatal violence,such as aggression,bullying,and physical fighting,are common forms of interpersonal violence between adolescents[1].展开更多
Traditional Chinese medicine(TCM)chiropractic massage is a kind of orthopedic therapy that corrects the abnormal anatomical position of bone or joint and corrects the dislocation of joint by manipulation so that the b...Traditional Chinese medicine(TCM)chiropractic massage is a kind of orthopedic therapy that corrects the abnormal anatomical position of bone or joint and corrects the dislocation of joint by manipulation so that the bone is soft and the Qi and blood are reconciled(Qi:The intangible,high-mobility nutritive substance that maintains vital activities).Manipulation treatment is the first of the four major orthopedic diseases.At the same time,TCM chiropractic massage is also an essential part of TCM.TCM orthopedic chiropractic practice originated from the struggle between humanity and nature,sprouted from the Warring States period to the two Han Dynasty,formed in the Sui,Tang,and Song Dynasties,matured in the Ming and Qing Dynasties,and flourished in the modern era.In more than 2,000 years of development,many practical techniques have been produced,which have profoundly influenced the inheritance and development of modern techniques,making the TCM orthopedic chiropractic massage in the contemporary era form the characteristics of“tendon and bone”and“point-line-face combination”.At present,the development of TCM chiropractic massage is facing the great challenges of modern medicine and modern advanced science and technology such as American chiropractic and the limitations of its development.Therefore,in the future,the development direction of Chinese medicine orthopedic chiropractic massage should be“hybrid to treat”.In the face of modern medicine,we should take its essence to its dross,eclectic,organically integrate its advantages into itself,enrich its theoretical framework,and strengthen the construction of disciplines and departments to enhance its competitiveness.It is believed that Chinese medicine orthopedic chiropractic massage can have better development in the future and can make more contributions to the health of the world’s people.展开更多
Background:Invasive Trichosporon asahii(T.asahii)infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of T.asahii infection remains elusive.Circular RNAs(circRNAs)are a type ...Background:Invasive Trichosporon asahii(T.asahii)infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of T.asahii infection remains elusive.Circular RNAs(circRNAs)are a type of endogenous noncoding RNA that participate in various disease processes.However,the mechanism of circRNAs in T.asahii infection remains completely unknown.Methods:RNA sequencing(RNA-seq)was performed to analyze the expression profiles of circRNAs,microRNAs(miRNAs),and mRNAs in THP-1 cells infected with T.asahii or uninfected samples.Some of the RNA-seq results were verified by RT-qPCR.Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were used to analyze the differentially expressed mRNAs.A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments.Results:A total of 46 circRNAs,412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T.asahii infection.GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response,the Toll-like receptor signaling pathway,and the TNF signaling pathway.A competing endogenous RNA(ceRNA)network was constructed with 5 differentially expressed circRNAs,5 differentially expressed miRNAs and 42 differentially expressed mRNAs.Among them,hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p.Conclusions:These data revealed a comprehensive circRNA-associated ceRNA network during T.asahii infection,thus providing new insights into the pathogenesis of the T.asahii-host interactions.展开更多
Esophageal cancer(EC)has a high incidence and mortality rate and is emerging as one of the most common health problems globally.Owing to the lack of sensitive detection methods,uncontrollable rapid metastasis,and perv...Esophageal cancer(EC)has a high incidence and mortality rate and is emerging as one of the most common health problems globally.Owing to the lack of sensitive detection methods,uncontrollable rapid metastasis,and pervasive treatment resistance,EC is often diagnosed in advanced stages and is susceptible to local recurrence.Exosomes are important components of intercellular communication and the exosome-mediated crosstalk between the cancer and surrounding cells within the tumor microenvironment plays a crucial role in the metastasis,progression,and therapeutic resistance of EC.Considering the critical role of exosomes in tumor pathogenesis,this review focused on elucidating the impact of exosomes on EC metastasis and therapeutic resistance.Here,we summarized the relevant signaling pathways involved in these processes.In addition,we discussed the potential clinical applications of exosomes for the early diagnosis,prognosis,and treatment of EC.展开更多
Hepatocellular carcinoma(HCC),one of the most common malignant tumors in China,severely threatens the life and health of patients.In recent years,precision medicine,clinical diagnoses,treatments,and innovative researc...Hepatocellular carcinoma(HCC),one of the most common malignant tumors in China,severely threatens the life and health of patients.In recent years,precision medicine,clinical diagnoses,treatments,and innovative research have led to important breakthroughs in HCC care.The discovery of new biomarkers and the promotion of liquid biopsy technologies have greatly facilitated the early diagnosis and treatment of HCC.Progress in targeted therapy and immunotherapy has provided more choices for precise HCC treatment.Multiomics technologies,such as genomics,transcriptomics,and metabolomics,have enabled deeper understanding of the occurrence and development mechanisms,heterogeneity,and genetic mutation characteristics of HCC.The continued promotion and accurate typing of HCC,accurate guidance of treatment,and accurate prognostication have provided more treatment opportunities and prolonged survival timelines for patients with HCC.Innovative HCC research providing an in-depth understanding of the biological characteristics of HCC will be translated into accurate clinical practices for the diagnosis and treatment of HCC.展开更多
BACKGROUND Primary bone lymphoma(PBL)is an uncommon extranodal disease that represents approximately 1%-3%of lymphomas.Anaplastic lymphoma kinase(ALK)positive anaplastic large-cell lymphoma(ALCL)is an extremely rare t...BACKGROUND Primary bone lymphoma(PBL)is an uncommon extranodal disease that represents approximately 1%-3%of lymphomas.Anaplastic lymphoma kinase(ALK)positive anaplastic large-cell lymphoma(ALCL)is an extremely rare type of PBL.The aim of this report is describe the symptoms,diagnosis,and treatment of primary bone ALK-positive ALCL.CASE SUMMARY A 66-year-old man presented to our hospital with neck and shoulder pain and intermittent fever that lasted for 1 mo.After extensive evaluation,positron emission tomography-computed tomography(CT)examination showed multiple osteolytic bone lesions without other sites lesions.CT-guided biopsy of the T10 vertebral body was performed,and the pathology results showed that neoplastic cells were positive for ALK-1,CD30,and CD3.A diagnosis of primary bone ALK positive ALCL was ultimately made.The patient was in partial response after four cycle soft cyclophosphamide,doxorubicin,vincristine,and prednisone chemotherapy,and we planned to repeat the biopsy and radiological examination after completion of the fifth cycle of therapy.CONCLUSION Primary bone ALK positive ALCL is a rare disease and physicians should keep in mind that ALCL can present with isolated osseous involvement without nodal involvement,and lymphoma should be considered in the differential diagnosis of primary bone lesions.展开更多
BACKGROUND Little is known about the postoperative sleep quality of infective endocarditis patients during hospitalization and after discharge.AIM To investigate the sleep characteristics of infective endocarditis pat...BACKGROUND Little is known about the postoperative sleep quality of infective endocarditis patients during hospitalization and after discharge.AIM To investigate the sleep characteristics of infective endocarditis patients and to identify potential risk factors for disturbed sleep quality after surgery.METHODS The Pittsburgh Sleep Quality Index(PSQI)and the Epworth Sleepiness Scale were used to assess patient sleep quality.Logistic regression was used to explore the potential risk factors.RESULTS The study population(n=139)had an average age of 43.40±14.56 years,and 67.6%were men(n=94).Disturbed sleep quality was observed in 86 patients(61.9%)during hospitalization and remained in 46 patients(33.1%)at 6 mo after surgery.However,both PSQI and Epworth Sleepiness Scale scores showed significant improvements at 6 mo(P<0.001 and P=0.001,respectively).Multivariable logistic regression analysis showed that the potential risk factors were age(odds ratio=1.125,95%confidence interval:1.068-1.186)and PSQI assessed during hospitalization(odds ratio=1.759,95%confidence interval:1.436-2.155).The same analysis in patients with PSQI≥8 during hospitalization suggested that not using sleep medication(odds ratio=15.893,95%confidence interval:2.385-105.889)may be another risk factor.CONCLUSION The incidence of disturbed sleep after infective endocarditis surgery is high.However,the situation improves significantly over time.Age and early postoperative high PSQI score are risk factors for disturbed sleep quality at 6 mo after surgery.展开更多
Acute respiratory distress syndrome(ARDS)is a common and clinically devastating disease that causes respiratory failure.Morbidity and mortality of patients in intensive care units are stubbornly high,and various compl...Acute respiratory distress syndrome(ARDS)is a common and clinically devastating disease that causes respiratory failure.Morbidity and mortality of patients in intensive care units are stubbornly high,and various complications severely affect the quality of life of survivors.The pathophysiology of ARDS includes increased alveolar–capillary membrane permeability,an influx of protein-rich pulmonary edema fluid,and surfactant dysfunction leading to severe hypoxemia.At present,the main treatment for ARDS is mechanical treatment combined with diuretics to reduce pulmonary edema,which primarily improves symptoms,but the prognosis of patients with ARDS is still very poor.Mesenchymal stem cells(MSCs)are stromal cells that possess the capacity to self-renew and also exhibit multilineage differentiation.MSCs can be isolated from a variety of tissues,such as the umbilical cord,endometrial polyps,menstrual blood,bone marrow,and adipose tissues.Studies have confirmed the critical healing and immunomodulatory properties of MSCs in the treatment of a variety of diseases.Recently,the potential of stem cells in treating ARDS has been explored via basic research and clinical trials.The efficacy of MSCs has been shown in a variety of in vivo models of ARDS,reducing bacterial pneumonia and ischemia-reperfusion injury while promoting the repair of ventilator-induced lung injury.This article reviews the current basic research findings and clinical applications of MSCs in the treatment of ARDS in order to emphasize the clinical prospects of MSCs.展开更多
Objective Vitamin D(VD)deficiency was reported to contribute to the progression of Crohn’s disease(CD)and affect the prognosis of CD patients.This study investigated the role of serum VD,body mass index(BMI),and tumo...Objective Vitamin D(VD)deficiency was reported to contribute to the progression of Crohn’s disease(CD)and affect the prognosis of CD patients.This study investigated the role of serum VD,body mass index(BMI),and tumor necrosis factor alpha(TNF-α)in the diagnosis of Crohn’s disease.Methods CD patients(n=76)and healthy subjects(n=76)were enrolled between May 2019 and December 2020.The serum 25-hydroxyvitamin D[25(OH)D]levels,BMI,and TNF-αlevels,together with other biochemical parameters,were assessed before treatment.The diagnostic efficacy of the single and joint detection of serum 25(OH)D,BMI,and TNF-αwas determined using receiver operating characteristic(ROC)curves.Results The levels of 25(OH)D,BMI,and nutritional indicators,including hemoglobin,total protein,albumin,and high-density lipoprotein cholesterol,were much lower,and the TNF-αlevels were much higher in the CD patients than in the healthy subjects(P<0.05 for all).The areas under the ROC curve for the single detection of 25(OH)D,BMI,and TNF-αwere 0.887,0.896,and 0.838,respectively,with the optimal cutoff values being 20.64 ng/mL,19.77 kg/m^(2),and 6.85 fmol/mL,respectively.The diagnostic efficacy of the joint detection of 25(OH)D,BMI,and TNF-αwas the highest,with an area under the ROC curve of 0.988(95%CI:0.968–1.000).Conclusion The joint detection of 25(OH)D,TNF-α,and BMI showed high sensitivity,specificity,and accuracy in CD diagnosis;thus,it would be effective for the diagnosis of CD in clinical practice.展开更多
As the major source of energy for colonic mucosal cells and as an important regulator of gene expression,inflammation,differentiation,and apoptosis in host cells,microbiota-derived butyrate can enhance the intestinal ...As the major source of energy for colonic mucosal cells and as an important regulator of gene expression,inflammation,differentiation,and apoptosis in host cells,microbiota-derived butyrate can enhance the intestinal mucosal immune barrier,modulate systemic immune response,and prevent infections.Maintaining a certain level of butyrate production in the gut can help balance intestinal microbiota,regulate host immune response,and promote the development and maintenance of the intestinal mucosal barrier.Butyrate-producing bacteria act as probiotics and play important roles in a variety of normal biological functions.Bacteriotherapeutic supplementation by using fecal microbiota transplantation to restore butyrate-producing commensal bacteria in the gut has been very successful in the treatment of recurrent and refractory Clostridium difficile(C.difficile)infection or C.difficile-negative nosocomial diarrhea.Administration of probiotics that include butyrate-producing bacteria may have a role in the treatment of inflammatory bowel diseases and in the prevention of necrotizing enterocolitis and late-onset sepsis in premature infants.Furthermore,modulating gut microbiota with dietary approaches may improve intestinal dysbiosis commonly seen in patients with obesity-associated metabolic disorders.Supplementation with a butyrate-producing bacterial stain might be used to increase energy expenditure,improve insulin sensitivity,and to help control obesity and metabolic syndrome.展开更多
Objective:This study aims to evaluate the morphology and function of the aortic valve after transcatheter closure of ventricular septal defect(VSD)with aortic valve prolapse(AVP)abased on clinical and radiological out...Objective:This study aims to evaluate the morphology and function of the aortic valve after transcatheter closure of ventricular septal defect(VSD)with aortic valve prolapse(AVP)abased on clinical and radiological outcomes.Methods:From January 2013 to November 2014,164 consecutive patients(97 males,59.1%)with VSD and AVP were treated by transcatheter closure.The patients were divided into the mild AVP group(n=63),moderate AVP group(n=89)and severe AVP group(n=12).The clinical and radiological outcomes of these patients were analyzed retrospectively.Results:In total,146(89.0%)patients were successfully treated with VSD occluders,including 59/63(93.7%)with mild AVP,80/89(89.9%)with moderate AVP and 7/12(58.3%)with severe AVP.The degree of AVP was ameliorated or disappeared in 39(26.7%)patients,and remained unchanged in 103(70.5%)patients after the intervention.In the 35 patients who initially had trivial-to-moderate aortic regurgitation(AR),the degree of AR was ameliorated or disappeared in 25(71.4%)patients,aggravated from trivial to mild AR in 1(2.9%)patient,and remained unchanged in 9(25.7%)patients.In 111 patients without AR,1(0.9%)patient had mild AR and 24(21.6%)patients had trivial AR after intervention.The depth and width of the prolapsed aortic valve decreased after transcatheter closure of VSD in all three groups.During the 70-month(range,54–77)follow-up period,no patients with AVP and AR needed an aortic valve intervention.Conclusions:Transcatheter closure of VSD with AVP is feasible.The morphology and function of the prolapsed aortic valve improved and remained stable for a long period after intervention.展开更多
Objective:The incidence of adenocarcinoma of the esophagogastric junction(AEG)is increasing.For patients with resectable Siewert II AEG,there is still a lack of consensus with regards to which surgical procedure can w...Objective:The incidence of adenocarcinoma of the esophagogastric junction(AEG)is increasing.For patients with resectable Siewert II AEG,there is still a lack of consensus with regards to which surgical procedure can well balance survival time and quality of life.This study aims to describe a modified double-tract reconstruction(DTR)method following laparoscopic proximal gastrectomy(LPG)and to evaluate the feasibility,safety,and effects of this novel method.Method:This study retrospectively reviewed 29 patients who received modified DTR after LPG for Siewert Ⅱ AEG at a single center between August 2015 and October 2020.Clinicopathological characteristics,surgical outcomes,nutritional status and dietary ability were analyzed.Result:The mean surgical time was 206.4±39.2 min,mean intraoperative blood loss was 175.9±41.4 mL,and median postoperative hospital stay was 11 d(range,9-70 d).The early complication rate was 13.8%(n=4),and the late complication rate was 6.9%(n=2).No reflux esophagitis or reflux symptom was recorded.There were no perioperative deaths within three months post-operatively.At the third follow-up month after surgery,most patients(25/29,86.2%)were experiencing sufficient oral food intake,with a median frequency of 5 meal/d(range,3-6 meal/d).The median total weight loss was 8 kg(range,0-15 kg)and there were no case of post-operative malnutrition.Conclusion:The modified DTR method following LPG represents a novel,safe,and feasible method that provides sufficient nutritional support with few diet-related discomforts.Prospective large-scale randomized trials are now needed to validate the clinical applicability of this method.展开更多
The exon numbers and lengths vary in different eukaryotic species. With increasing completed genomic sequences, it is indispensable to reanalyze the gene organization in diverse eukaryotic genomes. We performed a larg...The exon numbers and lengths vary in different eukaryotic species. With increasing completed genomic sequences, it is indispensable to reanalyze the gene organization in diverse eukaryotic genomes. We performed a large-scale comparative analysis of the exon-intron structure in 72 eukaryotic organisms, including plants, fungi and animals. We confirmed that the exon-intron structure varies massively among eukaryotic genomes and revealed some lineage-specific features of eukaryotic genes. These include a teleost-specific exon-intron structure pattern, relatively small introns and large exons in fungi and algae, and a gradual expansion of introns in vertebrates. Furthermore, the conservation analysis of exon-intron boundaries indicates that several bases near splice site junctions are different in introns with variable length among different species. After comparison, we identified a trend showing increases in intron densities and lengths in diverse species from fungi, plants, invertebrates to vertebrates, while it was the opposite in relation to exon lengths. The statistical properties of eukaryotic genomic organization suggest that genome-specific features are preserved by diverse evolutionary processes, which paves way for further research on the diversification of eukaryotic evolution.展开更多
The current animal models of stroke primarily model a single intracerebral hemorrhage(ICH)attack,and there is a lack of a reliable model of recurrent ICH.In this study,we established 16-month-old C57 B L/6 male mouse ...The current animal models of stroke primarily model a single intracerebral hemorrhage(ICH)attack,and there is a lack of a reliable model of recurrent ICH.In this study,we established 16-month-old C57 B L/6 male mouse models of ICH by injecting collagenaseⅦ-S into the left striatum.Twenty-one days later,we injected collagenaseⅦ-S into the right striatum to simulate recurrent ICH.Our results showed that mice subjected to bilateral striatal hemorrhage had poorer neurological function at the early stage of hemorrhage,delayed recovery in locomotor function,motor coordination,and movement speed,and more obvious emotional and cognitive dysfunction than mice subjected to unilate ral striatal hemorrhage.These findings indicate that mouse models of bilateral striatal hemorrhage can well simulate clinically common recurrent ICH.These models should be used as a novel tool for investigating the pathogenesis and treatment targets of recurrent ICH.展开更多
Background: Although socioeconomic support is recommended for frailtymanagement, its association with the prognosis of frailty is unclear.Methods: Using data from participants aged ≥65 years in the ChineseLongitudina...Background: Although socioeconomic support is recommended for frailtymanagement, its association with the prognosis of frailty is unclear.Methods: Using data from participants aged ≥65 years in the ChineseLongitudinal Healthy Longevity Survey (2008–2018), the associations betweensocioeconomic support (source of income, medical insurance, communitysupport, living status), onset of prefrailty/frailty, and worsening of prefrailty,were analyzed using multinominal logistic regression models. The associationsbetween self-reported low quality of life (QoL) and reversion of prefrailty/frailty were analyzed using multivariate logistic regression models. Associationswith mortality risk were analyzed using Cox proportional hazardregression models.Results: A total of 13,859 participants (mean age: 85.8 ± 11.1 years) containing2056 centenarians were included. Financial dependence was a risk factor for lowQoL among prefrail/frail individuals, but not among robust individuals. Havingcommercial or other insurance, and receiving social support from the communitywere protective factors for low QoL among prefrail/frail individuals and for theworsening of prefrailty. Continuing to work was a risk factor for low QoL, but aprotective factor for worsening of prefrailty. A negative association betweencontinuing to work and mortality existed in prefrail individuals aged <85 yearsand ≥85 years. Living alone was a risk factor for low QoL, but was notsignificantly associated with frailty prognosis.Conclusions: Prefrail and frail individuals were vulnerable to changes insocioeconomic support and more sensitive to it compared with robustindividuals. Preferential policies regarding financial support, social support,and medical insurance should be developed for individuals with frailty.展开更多
This study was designed to investigate the protective effects of Astaxanthin(AST)in rats with diabetes mellitus(DM)induced by streptozotocin.SD rats were divided into control group(n=5,only received normal saline),DM ...This study was designed to investigate the protective effects of Astaxanthin(AST)in rats with diabetes mellitus(DM)induced by streptozotocin.SD rats were divided into control group(n=5,only received normal saline),DM group(n=8)and AST+DM group(n=8;AST:50 mg/kg/day).DM rats were induced by intraperitoneal injection of streptozocin(STZ,65 mg/kg).Blood glucose level and body weight were determined at weeks 0,2,4,6 and 8,respectively.At week 8,kidney function was determined,together with expression of P53 and dynamin-related protein-1(Drp1)by Western blot analysis and immunofluorescence.AST led to increase of body weight in rats with DM.AST+DM group showed a significant decrease in blood glucose level at week 4 compared with DM group(P<0.05).AST improved renal function and significantly reduced expression of P53 and Drp1 in DM rats.In addition,AST can effectively reduce the blood glucose in DM rats,and delayed the pathogenesis of diabetic nephropathy.Such delay mediated by AST may be associated with the downregulation of Drp1 and P53.展开更多
基金Supported by National Natural Science Foundation of China,No.82303672Zhejiang Provincial Health Commission and Zhejiang Provincial Administration of Traditional Chinese Medicine through the Targeted Project for Medical and Health Research,No.2025ZL017and China Primary Health Care Foundation,No.ZLMY20240311001ZJ.
文摘BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically experience a poor prognosis,highlighting an urgent need to explore novel treatment approaches.This study evaluated the overall efficacy and safety of CMT vs CMT combined with immune checkpoint inhibitors(ICIs)in the treatment of EC patients with liver metastases.Furthermore,prognostic factors influencing outcomes in this patient population were identified.AIM To evaluate the efficacy and safety of first-line chemoimmunotherapy for EC patients with liver metastases and to analyze prognostic factors.METHODS This retrospective study included 126 EC patients with liver metastases at Zhejiang Cancer Hospital between 2014 and 2024.Patients receiving CMT were compared with those receiving CMT+ICI.Analyzed variables included clinicopathological features,treatment history,characteristics of metastasis,systemic and local treatments,overall survival(OS),and treatment-related adverse events(TRAEs).Prognostic factors were evaluated using univariate and multivariate Cox proportional-hazards regression models.Finally,efficacy outcomes and TRAE profiles were compared between the two groups.RESULTS A significant difference in median OS was identified between the two groups(10.8 months in the CMT group vs 20.8 months in the CMT+ICI group,P=0.004).The CMT+ICI group also demonstrated a significantly longer median progression-free survival of 11.7 months(P<0.001).Patients receiving combination therapy exhibited significantly improved systemic objective response rate and disease control rate.Multivariate analysis identified key factors significantly influencing OS in EC patients with liver metastases:Karnofsky Performance Status score≥70,receipt of local therapy for liver metastases,and the number of cycles of CMT and immunotherapy received.Furthermore,the incidence of TRAEs did not significantly differ between the CMT+ICI and CMT groups.CONCLUSION For EC patients with liver metastases,the combination of CMT and ICIs demonstrates significantly superior efficacy compared with CMT alone,while maintaining manageable TRAEs.
基金Supported by Guangdong Medical Science and Technology Research Fund Project,No.A2024475.
文摘This study explores the significance of using two-dimensional shear wave elastography(2D-SWE)to assess liver stiffness(LS)and spleen area(SPA)for predicting post-hepatectomy liver failure(PHLF).By providing a non-invasive method to measure LS,which correlates with the degree of liver fibrosis,and SPA,an indicator of portal hypertension,2D-SWE offers a comprehensive evaluation of a patient’s hepatic status.These advancements are particularly crucial in hepatic surgery,where accurate preoperative assessments are essential for optimizing surgical outcomes and minimizing complications.This letter highlights the prac-tical implications of integrating 2D-SWE into clinical practice,emphasizing its potential to improve patient safety and surgical precision by enhancing the ability to predict PHLF and tailor surgical approaches accordingly.
基金Key Research and Development Projects of Zhejiang Science and Technology Plan(No.2021C03103)Research and Development Plan of Zhejiang Science and Technology Department(No.2023C03089)Scientific Research Project of Zhejiang Provincial Department of Education(No.Y202456401)。
文摘Phakic intraocular lens implantation has become one of the important means of correcting refractive errors today.Among them,the implantable collamer lens(ICL)is favored for its wide range of correction,excellent optical quality,and high safety,but the risks of postoperative complications such as glaucoma and anterior subcapsular opacification still exist.Vault is an important indicator for evaluating the safety after ICL implantation,and its ideal state is crucial for preventing complications.Studies have shown that iris morphology has a significant impact on vault.In order to further optimize surgical outcomes and improve surgical safety,this review comprehensively reviews the research progress of iris-related parameters in ICL implantation and discusses the importance of various parameters in preoperative evaluation and postoperative follow-up.
基金Supported by Science and Technology Department of the State Administration of Traditional Chinese Medicine-Zhejiang Provincial Administration of Traditional Chinese Medicine Joint Science and Technology Program,No.GZY-ZJ-KJ-23057Bijie City Science and Technology Program 2023(Second Batch),No.BKH(2023)552024 Guizhou Province Chinese Medicine,Ethnic Medicine Science and Technology Research Special Project,No.QZYY-2024-109.
文摘BACKGROUND In recent years,the number of studies on spondylolisthesis has been increasing,and there are many publications on this disorder.To our knowledge,there is no bibliometric analysis of spondylolisthesis to date.AIM To investigate emerging directions in Spondylolisthesis research and systematically evaluate the academic literature with the highest citation impact within this field.METHODS All data were collected from the Web of Science Core Collection database.Years of publications,countries,journals,institutions and total number of citations were extracted and analyzed by VOSviewer software.In addition,we analyzed the top 100 most-cited articles on spondylolisthesis.RESULTS A total of 1831 articles related to spondylolisthesis were identified.The frequency of publications on spondylolisthesis has increased dramatically over time.Among all countries,United States has contributed the most publications on spondylolisthesis(n=574).The institution with the most articles was the University of California,San Francisco(n=52).Spine topped the list of journals and has published 291 spondylolisthesis-related reports.The hotspot of research changed from posterolateral fusion to interbody fusion.CONCLUSION In recent years,academic investigations on spondylolisthesis have exhibited significant growth.As the inaugural bibliometric evaluation in this domain,our research establishes a methodological framework for synthesizing the historical progression and current advancements of spondylolisthesis studies.
基金supported by grants from the National Nature Science Foundation of China 81874268,81573163,81673188College Students’ Innovation Project Foundation of Anhui Province in China grants number S201910366051。
文摘Self-inflicted and interpersonal violence are the two main types of adolescent violence.Individuals involved in self-inflicted violence both cause and receive the harm,while those who engage in interpersonal violence often aim to harm others.Self-harm and suicide are common types of selfinflicted violence.Non-fatal violence,such as aggression,bullying,and physical fighting,are common forms of interpersonal violence between adolescents[1].
基金funded by the National Natural Science Foundation(No.82460978,81860884)the Young and Middle-aged Academic and Technical Leaders Reserve Talents Project of Yunnan Province(grant No.202105AC160052)+1 种基金Technology Department-Applied Basic Research Joint Special Funds of the Yunnan University of Traditional Chinese Medicine(202301AZ070001-010)Scientific Research Fund of Education Department of Yunnan Province(2024Y415).
文摘Traditional Chinese medicine(TCM)chiropractic massage is a kind of orthopedic therapy that corrects the abnormal anatomical position of bone or joint and corrects the dislocation of joint by manipulation so that the bone is soft and the Qi and blood are reconciled(Qi:The intangible,high-mobility nutritive substance that maintains vital activities).Manipulation treatment is the first of the four major orthopedic diseases.At the same time,TCM chiropractic massage is also an essential part of TCM.TCM orthopedic chiropractic practice originated from the struggle between humanity and nature,sprouted from the Warring States period to the two Han Dynasty,formed in the Sui,Tang,and Song Dynasties,matured in the Ming and Qing Dynasties,and flourished in the modern era.In more than 2,000 years of development,many practical techniques have been produced,which have profoundly influenced the inheritance and development of modern techniques,making the TCM orthopedic chiropractic massage in the contemporary era form the characteristics of“tendon and bone”and“point-line-face combination”.At present,the development of TCM chiropractic massage is facing the great challenges of modern medicine and modern advanced science and technology such as American chiropractic and the limitations of its development.Therefore,in the future,the development direction of Chinese medicine orthopedic chiropractic massage should be“hybrid to treat”.In the face of modern medicine,we should take its essence to its dross,eclectic,organically integrate its advantages into itself,enrich its theoretical framework,and strengthen the construction of disciplines and departments to enhance its competitiveness.It is believed that Chinese medicine orthopedic chiropractic massage can have better development in the future and can make more contributions to the health of the world’s people.
基金the National Natural Science Foundation of China(No.81571972,81471928,81772138 and 82002120)the National Science Foundation of Beijing,China(No.7202201).
文摘Background:Invasive Trichosporon asahii(T.asahii)infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of T.asahii infection remains elusive.Circular RNAs(circRNAs)are a type of endogenous noncoding RNA that participate in various disease processes.However,the mechanism of circRNAs in T.asahii infection remains completely unknown.Methods:RNA sequencing(RNA-seq)was performed to analyze the expression profiles of circRNAs,microRNAs(miRNAs),and mRNAs in THP-1 cells infected with T.asahii or uninfected samples.Some of the RNA-seq results were verified by RT-qPCR.Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were used to analyze the differentially expressed mRNAs.A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments.Results:A total of 46 circRNAs,412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T.asahii infection.GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response,the Toll-like receptor signaling pathway,and the TNF signaling pathway.A competing endogenous RNA(ceRNA)network was constructed with 5 differentially expressed circRNAs,5 differentially expressed miRNAs and 42 differentially expressed mRNAs.Among them,hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p.Conclusions:These data revealed a comprehensive circRNA-associated ceRNA network during T.asahii infection,thus providing new insights into the pathogenesis of the T.asahii-host interactions.
基金Supported by the Natural Science Foundation of China,No.82003048 and No.82203284Joint Project of Natural Science Foundation of China,No.U22A2038+2 种基金Anhui Provincial Natural Science Foundation,No.1908085QC131 and No.2008085QH377Research Improvement Program of Anhui Medical University,No.2021xkjT002and Undergraduate Innovation and Entrepreneurship Training Program of Anhui Medical University,No.S202210366044.
文摘Esophageal cancer(EC)has a high incidence and mortality rate and is emerging as one of the most common health problems globally.Owing to the lack of sensitive detection methods,uncontrollable rapid metastasis,and pervasive treatment resistance,EC is often diagnosed in advanced stages and is susceptible to local recurrence.Exosomes are important components of intercellular communication and the exosome-mediated crosstalk between the cancer and surrounding cells within the tumor microenvironment plays a crucial role in the metastasis,progression,and therapeutic resistance of EC.Considering the critical role of exosomes in tumor pathogenesis,this review focused on elucidating the impact of exosomes on EC metastasis and therapeutic resistance.Here,we summarized the relevant signaling pathways involved in these processes.In addition,we discussed the potential clinical applications of exosomes for the early diagnosis,prognosis,and treatment of EC.
基金This study was supported by funding from the Science Technology and Innovation Committee of Shenzhen Municipality(Grant No.2019N002)Capital's Funds for Health Improvement and Research(Grant No.Z181100001718075).
文摘Hepatocellular carcinoma(HCC),one of the most common malignant tumors in China,severely threatens the life and health of patients.In recent years,precision medicine,clinical diagnoses,treatments,and innovative research have led to important breakthroughs in HCC care.The discovery of new biomarkers and the promotion of liquid biopsy technologies have greatly facilitated the early diagnosis and treatment of HCC.Progress in targeted therapy and immunotherapy has provided more choices for precise HCC treatment.Multiomics technologies,such as genomics,transcriptomics,and metabolomics,have enabled deeper understanding of the occurrence and development mechanisms,heterogeneity,and genetic mutation characteristics of HCC.The continued promotion and accurate typing of HCC,accurate guidance of treatment,and accurate prognostication have provided more treatment opportunities and prolonged survival timelines for patients with HCC.Innovative HCC research providing an in-depth understanding of the biological characteristics of HCC will be translated into accurate clinical practices for the diagnosis and treatment of HCC.
基金Supported by National Science and Technology Major Subproject of China,No.2018ZX10302205-002Chinese Foundation for Hepatitis Prevention and Control-Tianqing Liver Disease Research Fund Subject,No.TQGB2020168.
文摘BACKGROUND Primary bone lymphoma(PBL)is an uncommon extranodal disease that represents approximately 1%-3%of lymphomas.Anaplastic lymphoma kinase(ALK)positive anaplastic large-cell lymphoma(ALCL)is an extremely rare type of PBL.The aim of this report is describe the symptoms,diagnosis,and treatment of primary bone ALK-positive ALCL.CASE SUMMARY A 66-year-old man presented to our hospital with neck and shoulder pain and intermittent fever that lasted for 1 mo.After extensive evaluation,positron emission tomography-computed tomography(CT)examination showed multiple osteolytic bone lesions without other sites lesions.CT-guided biopsy of the T10 vertebral body was performed,and the pathology results showed that neoplastic cells were positive for ALK-1,CD30,and CD3.A diagnosis of primary bone ALK positive ALCL was ultimately made.The patient was in partial response after four cycle soft cyclophosphamide,doxorubicin,vincristine,and prednisone chemotherapy,and we planned to repeat the biopsy and radiological examination after completion of the fifth cycle of therapy.CONCLUSION Primary bone ALK positive ALCL is a rare disease and physicians should keep in mind that ALCL can present with isolated osseous involvement without nodal involvement,and lymphoma should be considered in the differential diagnosis of primary bone lesions.
基金Supported by Science and Technology Planning Project of Guangdong Province,No.A2016057.
文摘BACKGROUND Little is known about the postoperative sleep quality of infective endocarditis patients during hospitalization and after discharge.AIM To investigate the sleep characteristics of infective endocarditis patients and to identify potential risk factors for disturbed sleep quality after surgery.METHODS The Pittsburgh Sleep Quality Index(PSQI)and the Epworth Sleepiness Scale were used to assess patient sleep quality.Logistic regression was used to explore the potential risk factors.RESULTS The study population(n=139)had an average age of 43.40±14.56 years,and 67.6%were men(n=94).Disturbed sleep quality was observed in 86 patients(61.9%)during hospitalization and remained in 46 patients(33.1%)at 6 mo after surgery.However,both PSQI and Epworth Sleepiness Scale scores showed significant improvements at 6 mo(P<0.001 and P=0.001,respectively).Multivariable logistic regression analysis showed that the potential risk factors were age(odds ratio=1.125,95%confidence interval:1.068-1.186)and PSQI assessed during hospitalization(odds ratio=1.759,95%confidence interval:1.436-2.155).The same analysis in patients with PSQI≥8 during hospitalization suggested that not using sleep medication(odds ratio=15.893,95%confidence interval:2.385-105.889)may be another risk factor.CONCLUSION The incidence of disturbed sleep after infective endocarditis surgery is high.However,the situation improves significantly over time.Age and early postoperative high PSQI score are risk factors for disturbed sleep quality at 6 mo after surgery.
基金Supported by The Youth Fund project of the Natural Science Foundation of Zhejiang Province,No.LQ20H15010Zhejiang Traditional Chinese Medicine Science and Technology Planning Project,No.2023ZL575.
文摘Acute respiratory distress syndrome(ARDS)is a common and clinically devastating disease that causes respiratory failure.Morbidity and mortality of patients in intensive care units are stubbornly high,and various complications severely affect the quality of life of survivors.The pathophysiology of ARDS includes increased alveolar–capillary membrane permeability,an influx of protein-rich pulmonary edema fluid,and surfactant dysfunction leading to severe hypoxemia.At present,the main treatment for ARDS is mechanical treatment combined with diuretics to reduce pulmonary edema,which primarily improves symptoms,but the prognosis of patients with ARDS is still very poor.Mesenchymal stem cells(MSCs)are stromal cells that possess the capacity to self-renew and also exhibit multilineage differentiation.MSCs can be isolated from a variety of tissues,such as the umbilical cord,endometrial polyps,menstrual blood,bone marrow,and adipose tissues.Studies have confirmed the critical healing and immunomodulatory properties of MSCs in the treatment of a variety of diseases.Recently,the potential of stem cells in treating ARDS has been explored via basic research and clinical trials.The efficacy of MSCs has been shown in a variety of in vivo models of ARDS,reducing bacterial pneumonia and ischemia-reperfusion injury while promoting the repair of ventilator-induced lung injury.This article reviews the current basic research findings and clinical applications of MSCs in the treatment of ARDS in order to emphasize the clinical prospects of MSCs.
基金This research was funded by Guangzhou Science and Technology Plan Projects(No.202002020066)the Young Scientists to the NSFC Application of Guangdong Provincial People’s Hospital(No.8210120306)the Open Foundation of the State Key Laboratory of Bioactive Seaweed Substance(No.SKL-BMSG2022-03)。
文摘Objective Vitamin D(VD)deficiency was reported to contribute to the progression of Crohn’s disease(CD)and affect the prognosis of CD patients.This study investigated the role of serum VD,body mass index(BMI),and tumor necrosis factor alpha(TNF-α)in the diagnosis of Crohn’s disease.Methods CD patients(n=76)and healthy subjects(n=76)were enrolled between May 2019 and December 2020.The serum 25-hydroxyvitamin D[25(OH)D]levels,BMI,and TNF-αlevels,together with other biochemical parameters,were assessed before treatment.The diagnostic efficacy of the single and joint detection of serum 25(OH)D,BMI,and TNF-αwas determined using receiver operating characteristic(ROC)curves.Results The levels of 25(OH)D,BMI,and nutritional indicators,including hemoglobin,total protein,albumin,and high-density lipoprotein cholesterol,were much lower,and the TNF-αlevels were much higher in the CD patients than in the healthy subjects(P<0.05 for all).The areas under the ROC curve for the single detection of 25(OH)D,BMI,and TNF-αwere 0.887,0.896,and 0.838,respectively,with the optimal cutoff values being 20.64 ng/mL,19.77 kg/m^(2),and 6.85 fmol/mL,respectively.The diagnostic efficacy of the joint detection of 25(OH)D,BMI,and TNF-αwas the highest,with an area under the ROC curve of 0.988(95%CI:0.968–1.000).Conclusion The joint detection of 25(OH)D,TNF-α,and BMI showed high sensitivity,specificity,and accuracy in CD diagnosis;thus,it would be effective for the diagnosis of CD in clinical practice.
基金Supported by Medical and Health Science and Technology Plan of Zhejiang Province,No.2018KY128.
文摘As the major source of energy for colonic mucosal cells and as an important regulator of gene expression,inflammation,differentiation,and apoptosis in host cells,microbiota-derived butyrate can enhance the intestinal mucosal immune barrier,modulate systemic immune response,and prevent infections.Maintaining a certain level of butyrate production in the gut can help balance intestinal microbiota,regulate host immune response,and promote the development and maintenance of the intestinal mucosal barrier.Butyrate-producing bacteria act as probiotics and play important roles in a variety of normal biological functions.Bacteriotherapeutic supplementation by using fecal microbiota transplantation to restore butyrate-producing commensal bacteria in the gut has been very successful in the treatment of recurrent and refractory Clostridium difficile(C.difficile)infection or C.difficile-negative nosocomial diarrhea.Administration of probiotics that include butyrate-producing bacteria may have a role in the treatment of inflammatory bowel diseases and in the prevention of necrotizing enterocolitis and late-onset sepsis in premature infants.Furthermore,modulating gut microbiota with dietary approaches may improve intestinal dysbiosis commonly seen in patients with obesity-associated metabolic disorders.Supplementation with a butyrate-producing bacterial stain might be used to increase energy expenditure,improve insulin sensitivity,and to help control obesity and metabolic syndrome.
基金This study was reviewed and approved by the Institutional Review Board of Guangdong Province People’s Hospital(Guangzhou,Guangdong,China)(No.GDREC2020213H)This study was supported by National Key R&D Program of China(Grant No.2016YFC1100305)Sanming Medical Project of China(Grant No.SZSM201612057).
文摘Objective:This study aims to evaluate the morphology and function of the aortic valve after transcatheter closure of ventricular septal defect(VSD)with aortic valve prolapse(AVP)abased on clinical and radiological outcomes.Methods:From January 2013 to November 2014,164 consecutive patients(97 males,59.1%)with VSD and AVP were treated by transcatheter closure.The patients were divided into the mild AVP group(n=63),moderate AVP group(n=89)and severe AVP group(n=12).The clinical and radiological outcomes of these patients were analyzed retrospectively.Results:In total,146(89.0%)patients were successfully treated with VSD occluders,including 59/63(93.7%)with mild AVP,80/89(89.9%)with moderate AVP and 7/12(58.3%)with severe AVP.The degree of AVP was ameliorated or disappeared in 39(26.7%)patients,and remained unchanged in 103(70.5%)patients after the intervention.In the 35 patients who initially had trivial-to-moderate aortic regurgitation(AR),the degree of AR was ameliorated or disappeared in 25(71.4%)patients,aggravated from trivial to mild AR in 1(2.9%)patient,and remained unchanged in 9(25.7%)patients.In 111 patients without AR,1(0.9%)patient had mild AR and 24(21.6%)patients had trivial AR after intervention.The depth and width of the prolapsed aortic valve decreased after transcatheter closure of VSD in all three groups.During the 70-month(range,54–77)follow-up period,no patients with AVP and AR needed an aortic valve intervention.Conclusions:Transcatheter closure of VSD with AVP is feasible.The morphology and function of the prolapsed aortic valve improved and remained stable for a long period after intervention.
基金supported by the Beijing Municipal Natural Science Foundation(No.7204313)the Medical Big Data and Artificial Intelligence Project of Chinese PLA General Hospital(2019MBD-027).
文摘Objective:The incidence of adenocarcinoma of the esophagogastric junction(AEG)is increasing.For patients with resectable Siewert II AEG,there is still a lack of consensus with regards to which surgical procedure can well balance survival time and quality of life.This study aims to describe a modified double-tract reconstruction(DTR)method following laparoscopic proximal gastrectomy(LPG)and to evaluate the feasibility,safety,and effects of this novel method.Method:This study retrospectively reviewed 29 patients who received modified DTR after LPG for Siewert Ⅱ AEG at a single center between August 2015 and October 2020.Clinicopathological characteristics,surgical outcomes,nutritional status and dietary ability were analyzed.Result:The mean surgical time was 206.4±39.2 min,mean intraoperative blood loss was 175.9±41.4 mL,and median postoperative hospital stay was 11 d(range,9-70 d).The early complication rate was 13.8%(n=4),and the late complication rate was 6.9%(n=2).No reflux esophagitis or reflux symptom was recorded.There were no perioperative deaths within three months post-operatively.At the third follow-up month after surgery,most patients(25/29,86.2%)were experiencing sufficient oral food intake,with a median frequency of 5 meal/d(range,3-6 meal/d).The median total weight loss was 8 kg(range,0-15 kg)and there were no case of post-operative malnutrition.Conclusion:The modified DTR method following LPG represents a novel,safe,and feasible method that provides sufficient nutritional support with few diet-related discomforts.Prospective large-scale randomized trials are now needed to validate the clinical applicability of this method.
文摘The exon numbers and lengths vary in different eukaryotic species. With increasing completed genomic sequences, it is indispensable to reanalyze the gene organization in diverse eukaryotic genomes. We performed a large-scale comparative analysis of the exon-intron structure in 72 eukaryotic organisms, including plants, fungi and animals. We confirmed that the exon-intron structure varies massively among eukaryotic genomes and revealed some lineage-specific features of eukaryotic genes. These include a teleost-specific exon-intron structure pattern, relatively small introns and large exons in fungi and algae, and a gradual expansion of introns in vertebrates. Furthermore, the conservation analysis of exon-intron boundaries indicates that several bases near splice site junctions are different in introns with variable length among different species. After comparison, we identified a trend showing increases in intron densities and lengths in diverse species from fungi, plants, invertebrates to vertebrates, while it was the opposite in relation to exon lengths. The statistical properties of eukaryotic genomic organization suggest that genome-specific features are preserved by diverse evolutionary processes, which paves way for further research on the diversification of eukaryotic evolution.
基金supported by the Natural Science Foundation of Guangdong Province of China,No.2018A030313427the Science and Technology Program of Guangzhou of China,No.202002030393(both to LMW)。
文摘The current animal models of stroke primarily model a single intracerebral hemorrhage(ICH)attack,and there is a lack of a reliable model of recurrent ICH.In this study,we established 16-month-old C57 B L/6 male mouse models of ICH by injecting collagenaseⅦ-S into the left striatum.Twenty-one days later,we injected collagenaseⅦ-S into the right striatum to simulate recurrent ICH.Our results showed that mice subjected to bilateral striatal hemorrhage had poorer neurological function at the early stage of hemorrhage,delayed recovery in locomotor function,motor coordination,and movement speed,and more obvious emotional and cognitive dysfunction than mice subjected to unilate ral striatal hemorrhage.These findings indicate that mouse models of bilateral striatal hemorrhage can well simulate clinically common recurrent ICH.These models should be used as a novel tool for investigating the pathogenesis and treatment targets of recurrent ICH.
文摘Background: Although socioeconomic support is recommended for frailtymanagement, its association with the prognosis of frailty is unclear.Methods: Using data from participants aged ≥65 years in the ChineseLongitudinal Healthy Longevity Survey (2008–2018), the associations betweensocioeconomic support (source of income, medical insurance, communitysupport, living status), onset of prefrailty/frailty, and worsening of prefrailty,were analyzed using multinominal logistic regression models. The associationsbetween self-reported low quality of life (QoL) and reversion of prefrailty/frailty were analyzed using multivariate logistic regression models. Associationswith mortality risk were analyzed using Cox proportional hazardregression models.Results: A total of 13,859 participants (mean age: 85.8 ± 11.1 years) containing2056 centenarians were included. Financial dependence was a risk factor for lowQoL among prefrail/frail individuals, but not among robust individuals. Havingcommercial or other insurance, and receiving social support from the communitywere protective factors for low QoL among prefrail/frail individuals and for theworsening of prefrailty. Continuing to work was a risk factor for low QoL, but aprotective factor for worsening of prefrailty. A negative association betweencontinuing to work and mortality existed in prefrail individuals aged <85 yearsand ≥85 years. Living alone was a risk factor for low QoL, but was notsignificantly associated with frailty prognosis.Conclusions: Prefrail and frail individuals were vulnerable to changes insocioeconomic support and more sensitive to it compared with robustindividuals. Preferential policies regarding financial support, social support,and medical insurance should be developed for individuals with frailty.
基金This study was supported by Yantai Science and Technology Plan Project[Grant No.2018ZHGY088].
文摘This study was designed to investigate the protective effects of Astaxanthin(AST)in rats with diabetes mellitus(DM)induced by streptozotocin.SD rats were divided into control group(n=5,only received normal saline),DM group(n=8)and AST+DM group(n=8;AST:50 mg/kg/day).DM rats were induced by intraperitoneal injection of streptozocin(STZ,65 mg/kg).Blood glucose level and body weight were determined at weeks 0,2,4,6 and 8,respectively.At week 8,kidney function was determined,together with expression of P53 and dynamin-related protein-1(Drp1)by Western blot analysis and immunofluorescence.AST led to increase of body weight in rats with DM.AST+DM group showed a significant decrease in blood glucose level at week 4 compared with DM group(P<0.05).AST improved renal function and significantly reduced expression of P53 and Drp1 in DM rats.In addition,AST can effectively reduce the blood glucose in DM rats,and delayed the pathogenesis of diabetic nephropathy.Such delay mediated by AST may be associated with the downregulation of Drp1 and P53.
