The remaining uncertainties in relation to isovector nuclear interactions call for reliable experimental measurements of isovector probes in finite nuclei.Based on the Bayesian analysis,although neutron-skin thickness...The remaining uncertainties in relation to isovector nuclear interactions call for reliable experimental measurements of isovector probes in finite nuclei.Based on the Bayesian analysis,although neutron-skin thickness data or isovector giant dipole resonance data in^(208)Pb can constrain only one isovector interaction parameter,correlations among other parameters can also be built.Using combined data for both the neutron-skin thickness and the isovector giant dipole resonance helps to significantly constrain all isovector interaction parameters;as such,it serves as a useful methodology for future research.展开更多
With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versati...With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids(t FNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, t FNAs have been widely applied in the biomedical field as threedimensional DNA nanomaterials. Surprisingly, t FNAs exhibit positive effects on cellular biological behaviors and tissue regeneration,which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity,t FNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization,intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic t FNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic t FNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone,cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases.展开更多
Identifying patterns,recognition systems,prediction methods,and detection methods is a major challenge in solving different medical issues.Few categories of devices for personal and professional assessment of body com...Identifying patterns,recognition systems,prediction methods,and detection methods is a major challenge in solving different medical issues.Few categories of devices for personal and professional assessment of body composition are available.Bioelectrical impedance analyzer is a simple,safe,affordable,mobile,non-invasive,and less expensive alternative device for body composition assessment.Identifying the body composition pattern of different groups with varying age and gender is a major challenge in defining an optimal level because of the body shape,body mass,energy requirements,physical fitness,health status,and metabolic profile.Thus,this research aims to identify the statistical medical pattern recognition of body composition data by using a bioelectrical impedance analyzer.In previous studies,a pattern was identified for four indicators that concern body composition(e.g.,body mass index(BMI),body fat,muscle mass,and total body water).The novelty of our study is the fact that we identified a recognition pattern by using medical statistical methods for a body composition that contains seven indicators(e.g.,body fat,visceral fat,BMI,muscle mass,skeletal muscle mass,sarcopenic index,and total body water).The youth that exhibited the body composition pattern identified in our study could be considered healthy.Every deviation of one or more parameters outside the margins of the pattern for body composition could be associated with health issues,and more medical investigations would be needed for a diagnosis.BIA is considered a valid and reliable device to assess body composition along with medical statistical methods to identify a pattern for body composition according to the age,gender,and other relevant parameters.展开更多
A considerable efficiency gap exists between large-area perovskite solar modules and small-area perovskite solar cells.The control of forming uniform and large-area film and perovskite crystallization is still the mai...A considerable efficiency gap exists between large-area perovskite solar modules and small-area perovskite solar cells.The control of forming uniform and large-area film and perovskite crystallization is still the main obstacle restricting the efficiency of PSMs.In this work,we adopted a solid-liquid two-step film formation technique,which involved the evaporation of a lead iodide film and blade coating of an organic ammonium halide solution to prepare perovskite films.This method possesses the advantages of integrating vapor deposition and solution methods,which could apply to substrates with different roughness and avoid using toxic solvents to achieve a more uniform,large-area perovskite film.Furthermore,modification of the NiO_(x)/perovskite buried interface and introduction of Urea additives were utilized to reduce interface recombination and regulate perovskite crystallization.As a result,a large-area perovskite film possessing larger grains,fewer pinholes,and reduced defects could be achieved.The inverted PSM with an active area of 61.56 cm^(2)(10×10 cm^(2)substrate)achieved a champion power conversion efficiency of 20.56%and significantly improved stability.This method suggests an innovative approach to resolving the uniformity issue associated with large-area film fabrication.展开更多
Poly(lactic-co-glycolic)acid(PLGA)particles have become a commonly used drug delivery strategy in the pharmaceutical industry.In this work,we aim to investigate the size-dependent cellular internalization of PLGA part...Poly(lactic-co-glycolic)acid(PLGA)particles have become a commonly used drug delivery strategy in the pharmaceutical industry.In this work,we aim to investigate the size-dependent cellular internalization of PLGA particles and its effects on sustained drug release.We prepared three different-sized particles using PLGA(200,500 and 2000 nm)ranging from submicrometer to micrometer.Dexamethasone(DEX)with excellent anti-inflammatory properties was used as a model drug to prepare DEX-loaded PLGA particles(DPs).We comprehensively investigated the encapsulation efficiency,cellular uptake and in vitro drug release profile.Pharmacodynamic assessment revealed that,in the lipopolysaccharide(LPS)-induced RAW 264.7 cells model,500 nm DPs showed sustained anti-inflammatory efficacy.This work provides important information for designing PLGA-based drug delivery systems for biomedical applications.展开更多
Spherical nucleic acids(SNAs)are a 3D spherical nanostructure composed of highly oriented,dense layers of oligonucleotides conjugated to a hollow or solid core.This structure allows SNAs to show resistance to nuclease...Spherical nucleic acids(SNAs)are a 3D spherical nanostructure composed of highly oriented,dense layers of oligonucleotides conjugated to a hollow or solid core.This structure allows SNAs to show resistance to nuclease degradation,enter into nearly all cells without transfection agents and enable precise interactions with target molecules.Based on superior biological properties,SNAs can be tailored for diverse biological applications,rendering them a flexible and biosafe tool for biological applications as well as an enabling platform for therapy.In this review,we mainly discuss the structure and conjugation mode of SNAs and focus on recent advances in their applications,such as biomedical detection,imaging,and drug delivery.Finally,the remaining challenges and future directions of SNAs are also discussed and proposed.展开更多
Airborne diseases including SARS,bird flu,and the ongoing Coronavirus Disease 2019(COVID-19)have stimulated the demand for developing novel bioassay methods competent for early-stage diagnosis and large-scale screenin...Airborne diseases including SARS,bird flu,and the ongoing Coronavirus Disease 2019(COVID-19)have stimulated the demand for developing novel bioassay methods competent for early-stage diagnosis and large-scale screening.Here,we briefly summarize the state-of-the-art methods for the detection of infectious pathogens and discuss key challenges.We highlight the trend for next-generation technologies benefiting from multidisciplinary advances in microfabrication,nanotechnology and synthetic biology,which allow sensitive,rapid yet inexpensive pathogen assays with portable intelligent device.展开更多
In recent decades,DNA nanotechnology has grown into a highly innovative and widely established field.DNA nanostructures have extraordinary structural programmability and can accurately organize nanoscale materials,esp...In recent decades,DNA nanotechnology has grown into a highly innovative and widely established field.DNA nanostructures have extraordinary structural programmability and can accurately organize nanoscale materials,especially in guiding the synthesis of metal nanomaterials,which have unique advantages in controlling the growth morphology of metal nanomaterials.This review started with the evolution in DNA nanotechnology and the types of DNA nanostructures.Next,a DNA-based nanofabrication technology,DNA metallization,was introduced.In this section,we systematically summarized the DNA-oriented synthesis of metal nanostructures with different morphologies and structures.Furthermore,the applications of metal nanostructures constructed from DNA templates in various fields including electronics,catalysis,sensing,and bioimaging were figured out.Finally,the development prospects and challenges of metal nanostructures formed under the morphology control by DNA nanotechnology were discussed.展开更多
The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebol...The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebola,SARS,dengue,Zika,bird flu,and particularly the recent coronavirus disease 2019(COVID-19)caused by SARSCoV-2.Bioassays have been generally regarded as the first defense for the prevention and control of infectious diseases.Given the representative course of these diseases,bioassays against certain biomarkers are suitable for distinct stages of infection in diverse scenarios(Figure 1).However,the deployed bioassays still possess inherent limitations.Here,we briefly summarize the pros and cons of state-of-the-art bioassays for infectious diseases caused by pathogenic viruses and provide an outlook on the advances in the context of public health emergencies.展开更多
DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstandin...DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstanding plasticity and fluid thermodynamic properties,making them one of the best choices for mimicking natural biological tissues.By controlling the backbone building blocks,gelation conditions,and cross-linking methods of DNA hydrogels,hydrogels with different mechanical strengths can be obtained,thus expanding their applications in the field of biology.This review first introduces the relationship between the mechanical properties of DNA hydrogels and their structure,elucidates the approaches and strategies for mechanical property modulation,and focuses on the scheme of controllable design to modulate the mechanical properties of DNA hydrogels for applications in biosensing,cellular function regulation,and bone tissue engineering.Furthermore,this review outlines the future development directions and challenges faced in the mechanical property modulation of DNA hydrogels,providing useful information for the precise design of DNA hydrogels for biological research.展开更多
基金the National Natural Science Foundation of China(Grant No.11922514)。
文摘The remaining uncertainties in relation to isovector nuclear interactions call for reliable experimental measurements of isovector probes in finite nuclei.Based on the Bayesian analysis,although neutron-skin thickness data or isovector giant dipole resonance data in^(208)Pb can constrain only one isovector interaction parameter,correlations among other parameters can also be built.Using combined data for both the neutron-skin thickness and the isovector giant dipole resonance helps to significantly constrain all isovector interaction parameters;as such,it serves as a useful methodology for future research.
基金supported by National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(82101077,81970916)+3 种基金Sichuan Province Youth Science and Technology Innovation Team(2022JDTD0021)Sichuan University Postdoctoral Interdisciplinary Innovation Fundthe Fundamental Research Funds for the Central Universities,Postdoctoral Science Foundation of China(Grant 2021M692271)West China School/Hospital of Stomatology Sichuan University(No.RCDWJS2022-14 and RCDWJS2021-20)。
文摘With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids(t FNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, t FNAs have been widely applied in the biomedical field as threedimensional DNA nanomaterials. Surprisingly, t FNAs exhibit positive effects on cellular biological behaviors and tissue regeneration,which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity,t FNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization,intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic t FNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic t FNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone,cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases.
基金the APC was funded by“Stefan cel Mare”University of Suceava,Romania。
文摘Identifying patterns,recognition systems,prediction methods,and detection methods is a major challenge in solving different medical issues.Few categories of devices for personal and professional assessment of body composition are available.Bioelectrical impedance analyzer is a simple,safe,affordable,mobile,non-invasive,and less expensive alternative device for body composition assessment.Identifying the body composition pattern of different groups with varying age and gender is a major challenge in defining an optimal level because of the body shape,body mass,energy requirements,physical fitness,health status,and metabolic profile.Thus,this research aims to identify the statistical medical pattern recognition of body composition data by using a bioelectrical impedance analyzer.In previous studies,a pattern was identified for four indicators that concern body composition(e.g.,body mass index(BMI),body fat,muscle mass,and total body water).The novelty of our study is the fact that we identified a recognition pattern by using medical statistical methods for a body composition that contains seven indicators(e.g.,body fat,visceral fat,BMI,muscle mass,skeletal muscle mass,sarcopenic index,and total body water).The youth that exhibited the body composition pattern identified in our study could be considered healthy.Every deviation of one or more parameters outside the margins of the pattern for body composition could be associated with health issues,and more medical investigations would be needed for a diagnosis.BIA is considered a valid and reliable device to assess body composition along with medical statistical methods to identify a pattern for body composition according to the age,gender,and other relevant parameters.
基金the financial support from Shanxi Province Science and Technology Department(20201101012,202101060301016)the support from the APRC Grant of the City University of Hong Kong(9380086)+5 种基金the TCFS Grant(GHP/018/20SZ)MRP Grant(MRP/040/21X)from the Innovation and Technology Commission of Hong Kongthe Green Tech Fund(202020164)from the Environment and Ecology Bureau of Hong Kongthe GRF grants(11307621,11316422)from the Research Grants Council of Hong KongGuangdong Major Project of Basic and Applied Basic Research(2019B030302007)Guangdong-Hong Kong-Macao Joint Laboratory of Optoelectronic and Magnetic Functional Materials(2019B121205002).
文摘A considerable efficiency gap exists between large-area perovskite solar modules and small-area perovskite solar cells.The control of forming uniform and large-area film and perovskite crystallization is still the main obstacle restricting the efficiency of PSMs.In this work,we adopted a solid-liquid two-step film formation technique,which involved the evaporation of a lead iodide film and blade coating of an organic ammonium halide solution to prepare perovskite films.This method possesses the advantages of integrating vapor deposition and solution methods,which could apply to substrates with different roughness and avoid using toxic solvents to achieve a more uniform,large-area perovskite film.Furthermore,modification of the NiO_(x)/perovskite buried interface and introduction of Urea additives were utilized to reduce interface recombination and regulate perovskite crystallization.As a result,a large-area perovskite film possessing larger grains,fewer pinholes,and reduced defects could be achieved.The inverted PSM with an active area of 61.56 cm^(2)(10×10 cm^(2)substrate)achieved a champion power conversion efficiency of 20.56%and significantly improved stability.This method suggests an innovative approach to resolving the uniformity issue associated with large-area film fabrication.
基金supported by the National Natural Science Foundation of China (No.22022410,No.82050005,No.12105352)the Youth Innovation Promotion Association of Chinese Academy of Sciences (No.2012205,No.2016236).
文摘Poly(lactic-co-glycolic)acid(PLGA)particles have become a commonly used drug delivery strategy in the pharmaceutical industry.In this work,we aim to investigate the size-dependent cellular internalization of PLGA particles and its effects on sustained drug release.We prepared three different-sized particles using PLGA(200,500 and 2000 nm)ranging from submicrometer to micrometer.Dexamethasone(DEX)with excellent anti-inflammatory properties was used as a model drug to prepare DEX-loaded PLGA particles(DPs).We comprehensively investigated the encapsulation efficiency,cellular uptake and in vitro drug release profile.Pharmacodynamic assessment revealed that,in the lipopolysaccharide(LPS)-induced RAW 264.7 cells model,500 nm DPs showed sustained anti-inflammatory efficacy.This work provides important information for designing PLGA-based drug delivery systems for biomedical applications.
基金supported by the National Key Research and Development Program of China(2023YFA0915200)the National Natural Science Foundation of China(12305400,12105352)+1 种基金the Natural Science Foundation of Shanghai,China(22ZR1470600)the Xiangfu Lab Research Project(XF012022E0100).
文摘Spherical nucleic acids(SNAs)are a 3D spherical nanostructure composed of highly oriented,dense layers of oligonucleotides conjugated to a hollow or solid core.This structure allows SNAs to show resistance to nuclease degradation,enter into nearly all cells without transfection agents and enable precise interactions with target molecules.Based on superior biological properties,SNAs can be tailored for diverse biological applications,rendering them a flexible and biosafe tool for biological applications as well as an enabling platform for therapy.In this review,we mainly discuss the structure and conjugation mode of SNAs and focus on recent advances in their applications,such as biomedical detection,imaging,and drug delivery.Finally,the remaining challenges and future directions of SNAs are also discussed and proposed.
基金This work was financially supported by the National Natural Science Foundation of China(21991134,91953106)Science Foundation of the Shanghai Municipal Science and Technology Commission(19JC1410300,21dz2210100)。
文摘Airborne diseases including SARS,bird flu,and the ongoing Coronavirus Disease 2019(COVID-19)have stimulated the demand for developing novel bioassay methods competent for early-stage diagnosis and large-scale screening.Here,we briefly summarize the state-of-the-art methods for the detection of infectious pathogens and discuss key challenges.We highlight the trend for next-generation technologies benefiting from multidisciplinary advances in microfabrication,nanotechnology and synthetic biology,which allow sensitive,rapid yet inexpensive pathogen assays with portable intelligent device.
基金This work was supported by The Ministry of Science and Technology of China(2017YFA0205302)the NSFC(21922408)+2 种基金the Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars(BK20190038)Natural Science Foundation of Jiangsu Province-Major Project(BK20212012)Key Program of Natural Science Research in Universities of Jiangsu Province(19KJA360007).
文摘In recent decades,DNA nanotechnology has grown into a highly innovative and widely established field.DNA nanostructures have extraordinary structural programmability and can accurately organize nanoscale materials,especially in guiding the synthesis of metal nanomaterials,which have unique advantages in controlling the growth morphology of metal nanomaterials.This review started with the evolution in DNA nanotechnology and the types of DNA nanostructures.Next,a DNA-based nanofabrication technology,DNA metallization,was introduced.In this section,we systematically summarized the DNA-oriented synthesis of metal nanostructures with different morphologies and structures.Furthermore,the applications of metal nanostructures constructed from DNA templates in various fields including electronics,catalysis,sensing,and bioimaging were figured out.Finally,the development prospects and challenges of metal nanostructures formed under the morphology control by DNA nanotechnology were discussed.
基金supported by the National Natural Science Foundation of China(22074093)Science Foundation of the Shanghai Municipal Science and Technology Commission(21dz2210100)Shanghai Yangfan Program(21YF1459500).
文摘The outbreak of infectious diseases often arouses public health emergencies of international concern.During the past several decades,we have witnessed the outbreaks of various epidemics and pandemics,such as AIDS,Ebola,SARS,dengue,Zika,bird flu,and particularly the recent coronavirus disease 2019(COVID-19)caused by SARSCoV-2.Bioassays have been generally regarded as the first defense for the prevention and control of infectious diseases.Given the representative course of these diseases,bioassays against certain biomarkers are suitable for distinct stages of infection in diverse scenarios(Figure 1).However,the deployed bioassays still possess inherent limitations.Here,we briefly summarize the pros and cons of state-of-the-art bioassays for infectious diseases caused by pathogenic viruses and provide an outlook on the advances in the context of public health emergencies.
基金supported by the National Key Research and Development Program of China(2023YFB3208204)the National Natural Science Foundation of China(12305400,12105352)+2 种基金the Natural Science Foundation of Shanghai,China(22ZR1470600)the Natural Science Foundation of Shandong Province(ZR2019MB068,ZR2022MB012,ZR2021QE167)the Xiangfu Lab Research Project(XF012022E0100).
文摘DNA hydrogels are three-dimensional polymer networks constructed using DNA as the structural building block.Due to the tight binding between hydrophilic groups on DNA chains and water molecules,they exhibit outstanding plasticity and fluid thermodynamic properties,making them one of the best choices for mimicking natural biological tissues.By controlling the backbone building blocks,gelation conditions,and cross-linking methods of DNA hydrogels,hydrogels with different mechanical strengths can be obtained,thus expanding their applications in the field of biology.This review first introduces the relationship between the mechanical properties of DNA hydrogels and their structure,elucidates the approaches and strategies for mechanical property modulation,and focuses on the scheme of controllable design to modulate the mechanical properties of DNA hydrogels for applications in biosensing,cellular function regulation,and bone tissue engineering.Furthermore,this review outlines the future development directions and challenges faced in the mechanical property modulation of DNA hydrogels,providing useful information for the precise design of DNA hydrogels for biological research.
