Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulati...Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulating HAdV-21 strains in China.Methods:Whole-genome sequencing(WGS)was performed on 23 HAdV-21 strains isolated from acute respiratory infection cases,56.5%involving lower respiratory tract infections,across 7 Chinese sentinel surveillance provincial-level administrative divisions(PLADs)(2023-2024).These sequences,along with 50 previously reported HAdV-21 genomes from 6 countries(1956-2019),were integrated into a WGS dataset for comprehensive phylogenetic,genetic variation,and recombination analyses.Results:WGS categorized the HAdV-21 strains into 3 subtypes:HAdV-21a,HAdV-21b,and historical HAdV-21p(isolated in the 1950s).HAdV-21a(1956-2024,involving 5 of the 6 countries)and HAdV-21b(2005-2024,involving 3 of the 6 countries)exhibited extensive spatiotemporal distributions.Recent Chinese strains(2023-2024)belonged to HAdV-21a and HAdV-21b(HAdV-21a/b),showing extremely high genetic homology with Chinese 2019 strains(genetic distance:0.00007)and global strains(distance:<0.00040).Phylogenetic analysis confirmed that HAdV-21a/b shared a common ancestor and maintained a highly conserved genome despite decades of circulation.Sequence variation analysis identified shared and subtype-specific mutations in these two subtypes.Recombination pattern analysis further revealed that HAdV-21a/b acquired an HAdV-3-derived fragment in the E4 region(breakpoint:nt32,843).Conclusions:Recombinant HAdV-21a/b subtypes have co-circulated in China in recent years with remarkable genetic conservation.Enhanced surveillance is essential to quantify associated disease burden and guide targeted prevention and control strategies.展开更多
Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal...Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.展开更多
基金Supported by the National Key R&D Program of China(2022YFC2305303)and the Health and Wellness Sector Research Program of Gansu(GSWSON2021-010).
文摘Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulating HAdV-21 strains in China.Methods:Whole-genome sequencing(WGS)was performed on 23 HAdV-21 strains isolated from acute respiratory infection cases,56.5%involving lower respiratory tract infections,across 7 Chinese sentinel surveillance provincial-level administrative divisions(PLADs)(2023-2024).These sequences,along with 50 previously reported HAdV-21 genomes from 6 countries(1956-2019),were integrated into a WGS dataset for comprehensive phylogenetic,genetic variation,and recombination analyses.Results:WGS categorized the HAdV-21 strains into 3 subtypes:HAdV-21a,HAdV-21b,and historical HAdV-21p(isolated in the 1950s).HAdV-21a(1956-2024,involving 5 of the 6 countries)and HAdV-21b(2005-2024,involving 3 of the 6 countries)exhibited extensive spatiotemporal distributions.Recent Chinese strains(2023-2024)belonged to HAdV-21a and HAdV-21b(HAdV-21a/b),showing extremely high genetic homology with Chinese 2019 strains(genetic distance:0.00007)and global strains(distance:<0.00040).Phylogenetic analysis confirmed that HAdV-21a/b shared a common ancestor and maintained a highly conserved genome despite decades of circulation.Sequence variation analysis identified shared and subtype-specific mutations in these two subtypes.Recombination pattern analysis further revealed that HAdV-21a/b acquired an HAdV-3-derived fragment in the E4 region(breakpoint:nt32,843).Conclusions:Recombinant HAdV-21a/b subtypes have co-circulated in China in recent years with remarkable genetic conservation.Enhanced surveillance is essential to quantify associated disease burden and guide targeted prevention and control strategies.
基金This work is financially supported by the National Key Research and Development Program of China(No.2022YFF1101002)Jiangsu Association for Science and Technology Youth Science and Technology Talent Support Project(No.TJ-2021-049).
文摘Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.
