Background:Scutellaria baicalensis Georgi is a medicinal plant prized for its bioactive flavonoid derivatives.Flavonoid O-methyltransferases(OMTs)in this species play a vital role in enhancing these compounds’pharmac...Background:Scutellaria baicalensis Georgi is a medicinal plant prized for its bioactive flavonoid derivatives.Flavonoid O-methyltransferases(OMTs)in this species play a vital role in enhancing these compounds’pharmacological activities,including their antioxidant,anti-inflammatory,and anticancer effects.However,a comprehensive genomic overview of the OMT gene family in S.baicalensis is lacking.Methods:This study conducted a genome-wide identification of the OMT gene family in S.baicalensis using bioinformatics approaches.The identified genes were characterized through phylogenetic,physicochemical,and structural analyses.Furthermore,the response of methoxylated flavonoids and key SbOMT genes to drought stress was investigated.Results:A total of 54 SbOMTs were identified and classified into 9 CCoAOMT and 45 COMT subfamily members.These proteins,with lengths from 129 to 695 amino acids and molecular weights from 14.42 to 76.94 kDa,were predominantly acidic.Subcellular localization predicted 43% to be cytoplasmic.Structurally,the CCoAOMT subfamily was more conserved than the COMT subfamily.Promoter analysis revealed hormone-and stress-responsive cis-elements.Under drought stress,the root content of methoxylated flavonoids(wogonin,wogonoside,and oroxylin A)decreased initially and then increased.The expression of SbOMT06,SbOMT41,SbOMT27,and SbOMT29 was positively correlated with this accumulation,suggesting their involvement in biosynthesis.Conclusion:This study provides foundational insights into the SbOMT gene family,revealing key candidates likely involved in methoxyflavonoid biosynthesis.The findings advance our understanding of the molecular mechanisms in S.baicalensis and offer valuable resources for future metabolic engineering and pathway optimization efforts.展开更多
Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulati...Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulating HAdV-21 strains in China.Methods:Whole-genome sequencing(WGS)was performed on 23 HAdV-21 strains isolated from acute respiratory infection cases,56.5%involving lower respiratory tract infections,across 7 Chinese sentinel surveillance provincial-level administrative divisions(PLADs)(2023-2024).These sequences,along with 50 previously reported HAdV-21 genomes from 6 countries(1956-2019),were integrated into a WGS dataset for comprehensive phylogenetic,genetic variation,and recombination analyses.Results:WGS categorized the HAdV-21 strains into 3 subtypes:HAdV-21a,HAdV-21b,and historical HAdV-21p(isolated in the 1950s).HAdV-21a(1956-2024,involving 5 of the 6 countries)and HAdV-21b(2005-2024,involving 3 of the 6 countries)exhibited extensive spatiotemporal distributions.Recent Chinese strains(2023-2024)belonged to HAdV-21a and HAdV-21b(HAdV-21a/b),showing extremely high genetic homology with Chinese 2019 strains(genetic distance:0.00007)and global strains(distance:<0.00040).Phylogenetic analysis confirmed that HAdV-21a/b shared a common ancestor and maintained a highly conserved genome despite decades of circulation.Sequence variation analysis identified shared and subtype-specific mutations in these two subtypes.Recombination pattern analysis further revealed that HAdV-21a/b acquired an HAdV-3-derived fragment in the E4 region(breakpoint:nt32,843).Conclusions:Recombinant HAdV-21a/b subtypes have co-circulated in China in recent years with remarkable genetic conservation.Enhanced surveillance is essential to quantify associated disease burden and guide targeted prevention and control strategies.展开更多
Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal...Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.展开更多
基金funded by the National Natural Science Foundation of China(82404814,82404863)Start-up Research Fund of Nanjing Agricultural University(130-804141)+1 种基金the National Administration of Traditional Chinese Medicine High-level Key Discipline Construction Project(zyyzdxk-2023293)Scientific research Project of Jiangsu Institute of Product Quality Supervision and Inspection(KJ2025008).
文摘Background:Scutellaria baicalensis Georgi is a medicinal plant prized for its bioactive flavonoid derivatives.Flavonoid O-methyltransferases(OMTs)in this species play a vital role in enhancing these compounds’pharmacological activities,including their antioxidant,anti-inflammatory,and anticancer effects.However,a comprehensive genomic overview of the OMT gene family in S.baicalensis is lacking.Methods:This study conducted a genome-wide identification of the OMT gene family in S.baicalensis using bioinformatics approaches.The identified genes were characterized through phylogenetic,physicochemical,and structural analyses.Furthermore,the response of methoxylated flavonoids and key SbOMT genes to drought stress was investigated.Results:A total of 54 SbOMTs were identified and classified into 9 CCoAOMT and 45 COMT subfamily members.These proteins,with lengths from 129 to 695 amino acids and molecular weights from 14.42 to 76.94 kDa,were predominantly acidic.Subcellular localization predicted 43% to be cytoplasmic.Structurally,the CCoAOMT subfamily was more conserved than the COMT subfamily.Promoter analysis revealed hormone-and stress-responsive cis-elements.Under drought stress,the root content of methoxylated flavonoids(wogonin,wogonoside,and oroxylin A)decreased initially and then increased.The expression of SbOMT06,SbOMT41,SbOMT27,and SbOMT29 was positively correlated with this accumulation,suggesting their involvement in biosynthesis.Conclusion:This study provides foundational insights into the SbOMT gene family,revealing key candidates likely involved in methoxyflavonoid biosynthesis.The findings advance our understanding of the molecular mechanisms in S.baicalensis and offer valuable resources for future metabolic engineering and pathway optimization efforts.
基金Supported by the National Key R&D Program of China(2022YFC2305303)and the Health and Wellness Sector Research Program of Gansu(GSWSON2021-010).
文摘Introduction:Recent sentinel surveillance has revealed a rising prevalence of human adenovirus type 21(HAdV-21)among HAdV infections in China.This study aimed to elucidate the molecular features of currently circulating HAdV-21 strains in China.Methods:Whole-genome sequencing(WGS)was performed on 23 HAdV-21 strains isolated from acute respiratory infection cases,56.5%involving lower respiratory tract infections,across 7 Chinese sentinel surveillance provincial-level administrative divisions(PLADs)(2023-2024).These sequences,along with 50 previously reported HAdV-21 genomes from 6 countries(1956-2019),were integrated into a WGS dataset for comprehensive phylogenetic,genetic variation,and recombination analyses.Results:WGS categorized the HAdV-21 strains into 3 subtypes:HAdV-21a,HAdV-21b,and historical HAdV-21p(isolated in the 1950s).HAdV-21a(1956-2024,involving 5 of the 6 countries)and HAdV-21b(2005-2024,involving 3 of the 6 countries)exhibited extensive spatiotemporal distributions.Recent Chinese strains(2023-2024)belonged to HAdV-21a and HAdV-21b(HAdV-21a/b),showing extremely high genetic homology with Chinese 2019 strains(genetic distance:0.00007)and global strains(distance:<0.00040).Phylogenetic analysis confirmed that HAdV-21a/b shared a common ancestor and maintained a highly conserved genome despite decades of circulation.Sequence variation analysis identified shared and subtype-specific mutations in these two subtypes.Recombination pattern analysis further revealed that HAdV-21a/b acquired an HAdV-3-derived fragment in the E4 region(breakpoint:nt32,843).Conclusions:Recombinant HAdV-21a/b subtypes have co-circulated in China in recent years with remarkable genetic conservation.Enhanced surveillance is essential to quantify associated disease burden and guide targeted prevention and control strategies.
基金This work is financially supported by the National Key Research and Development Program of China(No.2022YFF1101002)Jiangsu Association for Science and Technology Youth Science and Technology Talent Support Project(No.TJ-2021-049).
文摘Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.
