Understanding how multivalency contributes to targeting dynamic lipid membranes is challenging.We show superselectivity in the multiple weak interactions formed between vesicles displaying nickel(II)-complexing nitril...Understanding how multivalency contributes to targeting dynamic lipid membranes is challenging.We show superselectivity in the multiple weak interactions formed between vesicles displaying nickel(II)-complexing nitrilotriacetic acid(NTA(Ni))and histidine(His)-displaying supported lipid bilayers(SLBs).The lateral mobility of receptors on the SLBs led to receptor recruitment inside the contact area between vesicle and SLB and depletion outside of the contact area in the case of low receptor density at the SLB and high ligand density in the targeting vesicles.展开更多
Natural hydrogels are one of the most promising biomaterials for tissue engineering applications,due to their biocompatibility,biodegradability,and extracellular matrix mimicking ability.To surpass the limitations of ...Natural hydrogels are one of the most promising biomaterials for tissue engineering applications,due to their biocompatibility,biodegradability,and extracellular matrix mimicking ability.To surpass the limitations of conventional fabrication techniques and to recapitulate the complex architecture of native tissue structure,natural hydrogels are being constructed using novel biofabrication strategies,such as textile techniques and three-dimensional bioprinting.These innovative techniques play an enormous role in the development of advanced scaffolds for various tissue engineering applications.The progress,advantages,and shortcomings of the emerging biofabrication techniques are highlighted in this review.Additionally,the novel applications of biofabricated natural hydrogels in cardiac,neural,and bone tissue engineering are discussed as well.展开更多
pplying biodegradable osteosyntheses avoids the disadvantages of titanium osteosyntheses. However, foreign-body reactions remain a major concern and evidence of complete resorption is lacking. This study compared the ...pplying biodegradable osteosyntheses avoids the disadvantages of titanium osteosyntheses. However, foreign-body reactions remain a major concern and evidence of complete resorption is lacking. This study compared the physico-chemical properties, histological response and radiographs of four copolymeric biodegradable osteo-synthesis systems in a goat model with 48-months follow-up. The systems were implanted subperiosteally in both tibia and radius of 12 Dutch White goats. The BioSorb FX [poly(70LLA-co-30DLLA)], Inion CPS [poly([70–78.5] LLA-co-[16–24]DLLA-co-4TMC)], SonicWeld Rx [poly(DLLA)], LactoSorb [poly(82LLA-co-18GA)] systems and a negative control were randomly implanted in each extremity. Samples were assessed at 6-, 12-, 18-, 24-, 36-, and 48-month follow-up. Surface topography was performed using scanning electron microscopy (SEM). Differential scanning calorimetry and gel permeation chromatography were performed on initial and explanted samples. Histological sections were systematically assessed by two blinded researchers using (polarized) light microscopy, SEM and energy-dispersive X-ray analysis. The SonicWeld Rx system was amorphous while the others were semi-crystalline. Foreign-body reactions were not observed during the complete follow-up. The SonicWeld Rx and LactoSorb systems reached bone percentages of negative controls after 18 months while the BioSorb Fx and Inion CPS systems reached these levels after 36 months. The SonicWeld Rx system showed the most predictable degradation profile. All the biodegradable systems were safe to use and well-tolerated (i.e., complete implant replacement by bone, no clinical or histological foreign body reactions, no [sterile] abscess formation, no re-interventions needed), but nanoscale residual polymeric fragments were observed at every system’s assessment.展开更多
Microfluidic systems enable automated and highly parallelized cell culture with low volumes and defined liquid dosing.To achieve this,systems typically integrate all functions into a single,monolithic device as a“one...Microfluidic systems enable automated and highly parallelized cell culture with low volumes and defined liquid dosing.To achieve this,systems typically integrate all functions into a single,monolithic device as a“one size fits all”solution.However,this approach limits the end users’(re)design flexibility and complicates the addition of new functions to the system.To address this challenge,we propose and demonstrate a modular and standardized plug-and-play fluidic circuit board(FCB)for operating microfluidic building blocks(MFBBs),whereby both the FCB and the MFBBs contain integrated valves.A single FCB can parallelize up to three MFBBs of the same design or operate MFBBs with entirely different architectures.The operation of the MFBBs through the FCB is fully automated and does not incur the cost of an extra external footprint.We use this modular platform to control three microfluidic large-scale integration(mLSI)MFBBs,each of which features 64 microchambers suitable for cell culturing with high spatiotemporal control.We show as a proof of principle that we can culture human umbilical vein endothelial cells(HUVECs)for multiple days in the chambers of this MFBB.Moreover,we also use the same FCB to control an MFBB for liquid dosing with a high dynamic range.Our results demonstrate that MFBBs with different designs can be controlled and combined on a single FCB.Our novel modular approach to operating an automated microfluidic system for parallelized cell culture will enable greater experimental flexibility and facilitate the cooperation of different chips from different labs.展开更多
基金the Netherlands Organisation for Scientific Research(grant no.VIDI 723.012.106).
文摘Understanding how multivalency contributes to targeting dynamic lipid membranes is challenging.We show superselectivity in the multiple weak interactions formed between vesicles displaying nickel(II)-complexing nitrilotriacetic acid(NTA(Ni))and histidine(His)-displaying supported lipid bilayers(SLBs).The lateral mobility of receptors on the SLBs led to receptor recruitment inside the contact area between vesicle and SLB and depletion outside of the contact area in the case of low receptor density at the SLB and high ligand density in the targeting vesicles.
基金the Ministry of Higher Education,Research and Innovation,France.A.Tamayol acknowledges the financial support from the National Institutes of Health,United States(GM126831,AR073822).
文摘Natural hydrogels are one of the most promising biomaterials for tissue engineering applications,due to their biocompatibility,biodegradability,and extracellular matrix mimicking ability.To surpass the limitations of conventional fabrication techniques and to recapitulate the complex architecture of native tissue structure,natural hydrogels are being constructed using novel biofabrication strategies,such as textile techniques and three-dimensional bioprinting.These innovative techniques play an enormous role in the development of advanced scaffolds for various tissue engineering applications.The progress,advantages,and shortcomings of the emerging biofabrication techniques are highlighted in this review.Additionally,the novel applications of biofabricated natural hydrogels in cardiac,neural,and bone tissue engineering are discussed as well.
文摘pplying biodegradable osteosyntheses avoids the disadvantages of titanium osteosyntheses. However, foreign-body reactions remain a major concern and evidence of complete resorption is lacking. This study compared the physico-chemical properties, histological response and radiographs of four copolymeric biodegradable osteo-synthesis systems in a goat model with 48-months follow-up. The systems were implanted subperiosteally in both tibia and radius of 12 Dutch White goats. The BioSorb FX [poly(70LLA-co-30DLLA)], Inion CPS [poly([70–78.5] LLA-co-[16–24]DLLA-co-4TMC)], SonicWeld Rx [poly(DLLA)], LactoSorb [poly(82LLA-co-18GA)] systems and a negative control were randomly implanted in each extremity. Samples were assessed at 6-, 12-, 18-, 24-, 36-, and 48-month follow-up. Surface topography was performed using scanning electron microscopy (SEM). Differential scanning calorimetry and gel permeation chromatography were performed on initial and explanted samples. Histological sections were systematically assessed by two blinded researchers using (polarized) light microscopy, SEM and energy-dispersive X-ray analysis. The SonicWeld Rx system was amorphous while the others were semi-crystalline. Foreign-body reactions were not observed during the complete follow-up. The SonicWeld Rx and LactoSorb systems reached bone percentages of negative controls after 18 months while the BioSorb Fx and Inion CPS systems reached these levels after 36 months. The SonicWeld Rx system showed the most predictable degradation profile. All the biodegradable systems were safe to use and well-tolerated (i.e., complete implant replacement by bone, no clinical or histological foreign body reactions, no [sterile] abscess formation, no re-interventions needed), but nanoscale residual polymeric fragments were observed at every system’s assessment.
基金This work was supported by the VESCEL ERC Advanced Grant to A.van den Berg(grant No.669768)the MFManufacturing ESCEL Joint Undertaking(grant No.621275-2)。
文摘Microfluidic systems enable automated and highly parallelized cell culture with low volumes and defined liquid dosing.To achieve this,systems typically integrate all functions into a single,monolithic device as a“one size fits all”solution.However,this approach limits the end users’(re)design flexibility and complicates the addition of new functions to the system.To address this challenge,we propose and demonstrate a modular and standardized plug-and-play fluidic circuit board(FCB)for operating microfluidic building blocks(MFBBs),whereby both the FCB and the MFBBs contain integrated valves.A single FCB can parallelize up to three MFBBs of the same design or operate MFBBs with entirely different architectures.The operation of the MFBBs through the FCB is fully automated and does not incur the cost of an extra external footprint.We use this modular platform to control three microfluidic large-scale integration(mLSI)MFBBs,each of which features 64 microchambers suitable for cell culturing with high spatiotemporal control.We show as a proof of principle that we can culture human umbilical vein endothelial cells(HUVECs)for multiple days in the chambers of this MFBB.Moreover,we also use the same FCB to control an MFBB for liquid dosing with a high dynamic range.Our results demonstrate that MFBBs with different designs can be controlled and combined on a single FCB.Our novel modular approach to operating an automated microfluidic system for parallelized cell culture will enable greater experimental flexibility and facilitate the cooperation of different chips from different labs.
