To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia ...To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia （C1N） and normal cervical tissues were studied. Using streptavidin-biotin peroxidase （S- P） immunohistochemical technique, the authors examined the expression of Bcl-2 and Survivin in these specimens. The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling （TUNEL） method. The infection of HPV type 16, 18 DNA were determined by PCR. It was found that there were significant differences in Bcl-2, Survivin and apoptotic index （AI） between cervical carcinomas, CIN and normal cervical tissues, respectively. Expression of Bcl-2 and AI were correlated with tu- mor grades, clinical stages and lymph node metastasis and expression of Survivin was associated with tu- mor grades and lymph node metastasis. There were different positive rate of HPV^s between cervical car- cinomas, C1N and normal tissues and were not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively. It was concluded that the abnormal expression of Bcl-2, Survivin and infection of HPV16/18 were associated with cervical carcinomas. They possibly can be useful indexes for the primary screening and prognosis of cervical carcinomas.展开更多
Metastasis is a leading cause of treatment failure and high mortality in non-small cell lung cancer(NSCLC).Recently,we demonstrated that DEAD box helicase 39B(DDX39B)was upregulated and activated metabolic reprogrammi...Metastasis is a leading cause of treatment failure and high mortality in non-small cell lung cancer(NSCLC).Recently,we demonstrated that DEAD box helicase 39B(DDX39B)was upregulated and activated metabolic reprogramming in colorectal cancer and hepatocellular carcinoma.However,the function of DDX39B and the therapeutic potential for targeting DDX39B in NSCLC remain unclear.Herein,we discovered that DDX39B was an independent marker for poor survival in NSCLC patients.Strikingly,DDX39B protein,but not its mRNA,was elevated in clinical metastatic brain lesions and metastatic cell models(in vitro EMT-metastatic and in vivo carotid artery injection-induced brain-metastatic cell model).展开更多
文摘To investigate the expression of Bcl-2 and Survivin and HPV16/18 infection in uterine cervical carcinogenesis, formalin-fixed, paraffin-embedded specimens from cervical carcinomas, cervical intraepithelial neoplasia （C1N） and normal cervical tissues were studied. Using streptavidin-biotin peroxidase （S- P） immunohistochemical technique, the authors examined the expression of Bcl-2 and Survivin in these specimens. The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling （TUNEL） method. The infection of HPV type 16, 18 DNA were determined by PCR. It was found that there were significant differences in Bcl-2, Survivin and apoptotic index （AI） between cervical carcinomas, CIN and normal cervical tissues, respectively. Expression of Bcl-2 and AI were correlated with tu- mor grades, clinical stages and lymph node metastasis and expression of Survivin was associated with tu- mor grades and lymph node metastasis. There were different positive rate of HPV^s between cervical car- cinomas, C1N and normal tissues and were not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively. It was concluded that the abnormal expression of Bcl-2, Survivin and infection of HPV16/18 were associated with cervical carcinomas. They possibly can be useful indexes for the primary screening and prognosis of cervical carcinomas.
基金supported by the National Natural Science Foundation of China(82103525,82272892,82404000)Department of Science and Technology of Sichuan Province(2024YFFK0383,2023ZYD0044)Postdoctor Research Fund of West China Hospital,Sichuan University(2024HXBH163).
文摘Metastasis is a leading cause of treatment failure and high mortality in non-small cell lung cancer(NSCLC).Recently,we demonstrated that DEAD box helicase 39B(DDX39B)was upregulated and activated metabolic reprogramming in colorectal cancer and hepatocellular carcinoma.However,the function of DDX39B and the therapeutic potential for targeting DDX39B in NSCLC remain unclear.Herein,we discovered that DDX39B was an independent marker for poor survival in NSCLC patients.Strikingly,DDX39B protein,but not its mRNA,was elevated in clinical metastatic brain lesions and metastatic cell models(in vitro EMT-metastatic and in vivo carotid artery injection-induced brain-metastatic cell model).
