The membrane-bound H<sup>+</sup>-ATPase of E. coil consisted of two parts: F<sub>1</sub> and F<sub>0</sub>. Purified ε subunit of F<sub>1</sub> strongly inhibited F<...The membrane-bound H<sup>+</sup>-ATPase of E. coil consisted of two parts: F<sub>1</sub> and F<sub>0</sub>. Purified ε subunit of F<sub>1</sub> strongly inhibited F<sub>1</sub> but showed no inhibitory effect in reconstituted F<sub>1</sub>F<sub>0</sub>. In 1987, G. B. Cox et al. isolated a mutant of ε subunit, and two partial revertants, Their experiments showed that F<sub>1</sub>F<sub>0</sub> complex was still formed in the mutant. The hydrolytic activity and transmembrane proton transfer ability were all impaired in the展开更多
BACKGROUND Adult-onset diabetes is most often considered to be type 2 diabetes.However,other types of diabetes can develop in adults,including exocrine pancreas diseaseassociated diabetes,also called type 3c diabetes....BACKGROUND Adult-onset diabetes is most often considered to be type 2 diabetes.However,other types of diabetes can develop in adults,including exocrine pancreas diseaseassociated diabetes,also called type 3c diabetes.Differential diagnosis between these types of diabetes still remains a diagnostic challenge.AIM To define anthropometric and laboratory markers that will allow for early diagnosis of pancreatic disease-associated diabetes.METHODS The study group included 44 patients with pancreatogenic diabetes(26 with pancreatic cancer and 18 with chronic pancreatitis),while the control group consisted of 35 patients with type 2 diabetes.We analyzed several parameters,including sex,age,body mass index(BMI),fasting plasma glucose,fasting Cpeptide and insulin with homeostasis model assessment of insulin resistance(HOMA-IR)index calculation,adrenomedullin,adiponectin and creatinine levels with epidermal growth factor receptor(eGFR)calculation.We also developed an equation,termed type 3c diabetes index,which utilized BMI,fasting insulin and adrenomedullin levels,and eGFR to better identify patients with type 3c diabetes.RESULTS Compared to patients with type 2 diabetes,patients with pancreatogenic diabetes had significantly lower BMI(25.11±4.87 kg/m^(2) vs 30.83±5.21 kg/m^(2)),fasting C-peptide(0.81±0.42 nmol/L vs 1.71±0.80 nmol/L),insulin(76.81±63.34 pmol/L vs 233.19±164.51 pmol/L)and HOMA-IR index,despite similar fasting plasma glucose levels.Patients with pancreatogenic diabetes also had lower adrenomedullin levels(0.41±0.25 ng/mL vs 0.63±0.38 ng/mL)but higher adiponectin levels(13.08±7.20μg/mL vs 8.28±4.01μg/mL)and eGFR levels(100.53±21.60 mL/min/1.73 m^(2) vs 85.14±19.24 mL/min/1.73 m^(2)).Finally,patients with pancreatogenic diabetes had significantly lower Type 3c diabetes index values.CONCLUSION Patients with pancreatogenic diabetes differ from patients with type 2 diabetes in anthropometric and laboratory parameters.The type 3c diabetes index had the highest discriminating value,above any single parameter.展开更多
In the framework of searching for new pectin sources to partially compensate for domestic and regional demands, the peel (albedo) of the “non-comestible” fruit of Poncirus trifoliata was investigated using a relativ...In the framework of searching for new pectin sources to partially compensate for domestic and regional demands, the peel (albedo) of the “non-comestible” fruit of Poncirus trifoliata was investigated using a relatively simple experimental design for optimization, in which only the variable was the extraction pH (1.0, 1.5, and 2.0) on the basis of our previous studies on diverse pectin sources. The results showed that the yield of pectin (7.4%-19.8%) was strongly influenced by the extraction pH when the other parameters, namely the solid to liquid extractant (S/L) ratio, temperature (T °C), and time (t) were fixed to 1:25 (w/v), 75°C, and 90 min, respectively. Likewise, the galacturonic acid content (GalA: 61.4%-79.2%), total neutral sugar content (TNS: 9.1%-22.5%), degree of branching (3.5%-13.9%), homogalacturonan (HG) to rhamnogalacturonan-I (RG-I) ratio (2.2-5.6), degree of methylesterification (DM: 54-77), viscosity average molecular weight (Mν: 57-82), and gelling capacity (GC: 124-158) were all affected by the extraction pH. The optimum pH for producing pectin with good yield, quality characteristics (GalA > 65%, DM > 60, Mν > 80 kDa), and gelling capacity (GC > 150), from the peel of P. trifoliata fruit, was found to be pH 1.5.展开更多
It is well established that adenosine triphosphatase （ATPase） complex is an important assembly for bioenergy transduction in the mitochondria from animal and microorganism. There were many reports on the triple stru...It is well established that adenosine triphosphatase （ATPase） complex is an important assembly for bioenergy transduction in the mitochondria from animal and microorganism. There were many reports on the triple structure of the coupling, the subunit composition and function as well as their interrelationship. Its head, i.e. the soluble coupling factor F1, had been separated from the ATPase complex, purified and recrystallized and its subunits had been separated and reconstituted. However,展开更多
The capillary flow in asymmetric interior corner consisting of straight vane and curved wall is studied with analytical solution.The concept of equivalent interior corner angle is proposed to convert the asymmetric in...The capillary flow in asymmetric interior corner consisting of straight vane and curved wall is studied with analytical solution.The concept of equivalent interior corner angle is proposed to convert the asymmetric interior corner model into symmetric interior corner model.Then the governing equations of interior corner flow are established,and based on which the interior corner flow is calculated.This method is used to analyze the capillary flow in cylindrical vane-type surface tension tank with outer vanes.The research can provide beneficial reference to the design of vane-type surface tension tank.展开更多
The subunits of plant virus capsids possess the inherent functional features which favor their polymerization to discs or particles. For keeping the subunits in the monomeric state, Van Regenmortel treated the coat pr...The subunits of plant virus capsids possess the inherent functional features which favor their polymerization to discs or particles. For keeping the subunits in the monomeric state, Van Regenmortel treated the coat protein with succinylation. Subsequently, he pointed out that although this treatment could keep the subunits展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘The membrane-bound H<sup>+</sup>-ATPase of E. coil consisted of two parts: F<sub>1</sub> and F<sub>0</sub>. Purified ε subunit of F<sub>1</sub> strongly inhibited F<sub>1</sub> but showed no inhibitory effect in reconstituted F<sub>1</sub>F<sub>0</sub>. In 1987, G. B. Cox et al. isolated a mutant of ε subunit, and two partial revertants, Their experiments showed that F<sub>1</sub>F<sub>0</sub> complex was still formed in the mutant. The hydrolytic activity and transmembrane proton transfer ability were all impaired in the
文摘BACKGROUND Adult-onset diabetes is most often considered to be type 2 diabetes.However,other types of diabetes can develop in adults,including exocrine pancreas diseaseassociated diabetes,also called type 3c diabetes.Differential diagnosis between these types of diabetes still remains a diagnostic challenge.AIM To define anthropometric and laboratory markers that will allow for early diagnosis of pancreatic disease-associated diabetes.METHODS The study group included 44 patients with pancreatogenic diabetes(26 with pancreatic cancer and 18 with chronic pancreatitis),while the control group consisted of 35 patients with type 2 diabetes.We analyzed several parameters,including sex,age,body mass index(BMI),fasting plasma glucose,fasting Cpeptide and insulin with homeostasis model assessment of insulin resistance(HOMA-IR)index calculation,adrenomedullin,adiponectin and creatinine levels with epidermal growth factor receptor(eGFR)calculation.We also developed an equation,termed type 3c diabetes index,which utilized BMI,fasting insulin and adrenomedullin levels,and eGFR to better identify patients with type 3c diabetes.RESULTS Compared to patients with type 2 diabetes,patients with pancreatogenic diabetes had significantly lower BMI(25.11±4.87 kg/m^(2) vs 30.83±5.21 kg/m^(2)),fasting C-peptide(0.81±0.42 nmol/L vs 1.71±0.80 nmol/L),insulin(76.81±63.34 pmol/L vs 233.19±164.51 pmol/L)and HOMA-IR index,despite similar fasting plasma glucose levels.Patients with pancreatogenic diabetes also had lower adrenomedullin levels(0.41±0.25 ng/mL vs 0.63±0.38 ng/mL)but higher adiponectin levels(13.08±7.20μg/mL vs 8.28±4.01μg/mL)and eGFR levels(100.53±21.60 mL/min/1.73 m^(2) vs 85.14±19.24 mL/min/1.73 m^(2)).Finally,patients with pancreatogenic diabetes had significantly lower Type 3c diabetes index values.CONCLUSION Patients with pancreatogenic diabetes differ from patients with type 2 diabetes in anthropometric and laboratory parameters.The type 3c diabetes index had the highest discriminating value,above any single parameter.
文摘In the framework of searching for new pectin sources to partially compensate for domestic and regional demands, the peel (albedo) of the “non-comestible” fruit of Poncirus trifoliata was investigated using a relatively simple experimental design for optimization, in which only the variable was the extraction pH (1.0, 1.5, and 2.0) on the basis of our previous studies on diverse pectin sources. The results showed that the yield of pectin (7.4%-19.8%) was strongly influenced by the extraction pH when the other parameters, namely the solid to liquid extractant (S/L) ratio, temperature (T °C), and time (t) were fixed to 1:25 (w/v), 75°C, and 90 min, respectively. Likewise, the galacturonic acid content (GalA: 61.4%-79.2%), total neutral sugar content (TNS: 9.1%-22.5%), degree of branching (3.5%-13.9%), homogalacturonan (HG) to rhamnogalacturonan-I (RG-I) ratio (2.2-5.6), degree of methylesterification (DM: 54-77), viscosity average molecular weight (Mν: 57-82), and gelling capacity (GC: 124-158) were all affected by the extraction pH. The optimum pH for producing pectin with good yield, quality characteristics (GalA > 65%, DM > 60, Mν > 80 kDa), and gelling capacity (GC > 150), from the peel of P. trifoliata fruit, was found to be pH 1.5.
文摘It is well established that adenosine triphosphatase （ATPase） complex is an important assembly for bioenergy transduction in the mitochondria from animal and microorganism. There were many reports on the triple structure of the coupling, the subunit composition and function as well as their interrelationship. Its head, i.e. the soluble coupling factor F1, had been separated from the ATPase complex, purified and recrystallized and its subunits had been separated and reconstituted. However,
基金supported by the National Natural Science Foundation of China (Grant No. 50975280)
文摘The capillary flow in asymmetric interior corner consisting of straight vane and curved wall is studied with analytical solution.The concept of equivalent interior corner angle is proposed to convert the asymmetric interior corner model into symmetric interior corner model.Then the governing equations of interior corner flow are established,and based on which the interior corner flow is calculated.This method is used to analyze the capillary flow in cylindrical vane-type surface tension tank with outer vanes.The research can provide beneficial reference to the design of vane-type surface tension tank.
文摘The subunits of plant virus capsids possess the inherent functional features which favor their polymerization to discs or particles. For keeping the subunits in the monomeric state, Van Regenmortel treated the coat protein with succinylation. Subsequently, he pointed out that although this treatment could keep the subunits
