A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signa...A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace levels; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.展开更多
Huperzine V, a new Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and the absolute stereochemistry was determined by X-ray crystallographic analysis.
Huperzine W, a novel 14 carbons Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and its stucture was determined by spectroscopic analysis.
Further phytochemical investigation of the ethyl acetate extract of the stem of Daphniphyllum oldhamii afforded a new phenolic glucoside, named oldhamioside 1, together with eleven known compounds, koaburaside 2, betu...Further phytochemical investigation of the ethyl acetate extract of the stem of Daphniphyllum oldhamii afforded a new phenolic glucoside, named oldhamioside 1, together with eleven known compounds, koaburaside 2, betulin, 28-hydroxyl-3-lupenone, pineresinol, syringaresinol, 4-O-methyl- cedrusin, narigenin, eriodictyol, apigenin, loureirin C, and asperuloside. The structures of new and known compounds were characterized by detailed spectroscopic analysis and comparison of their spectral data with reported values.展开更多
A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s...A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s camphor sultam as chiral auxiliary.展开更多
A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the a...A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the anti-osteoporosis agents.展开更多
The stereoselective synthesis of (2R, 4R)-2-N-tert-butyloxycarbonyl amino-4, 5-epoxido- valeric acid methyl ester 8, which is the key intermediate for the synthesis of (2S, 2R) -3-trans-nitrocyclopropyl-alanine, was f...The stereoselective synthesis of (2R, 4R)-2-N-tert-butyloxycarbonyl amino-4, 5-epoxido- valeric acid methyl ester 8, which is the key intermediate for the synthesis of (2S, 2R) -3-trans-nitrocyclopropyl-alanine, was first accomplished.展开更多
Two new steroids, nanjiols D and E, were isolated from the soft coral Nephthea bayeri. Their structures were characterized by spectroscopic methods and comparison with known compounds.
A novel alkaloid was isolated from the stems and leaves of Stemona parviflora Wright. Based on the spectral methods, its structure was elucidated as parvineostemonine.
AIM To evaluate the therapeutic effects of LLT on the type II collagen-induced arthritis in DBA/1 mice. LLT is a new compound derived from triptolide. LLT had been demonstrated in vitro and in vivo for its immunosuppr...AIM To evaluate the therapeutic effects of LLT on the type II collagen-induced arthritis in DBA/1 mice. LLT is a new compound derived from triptolide. LLT had been demonstrated in vitro and in vivo for its immunosuppressive activities, and our results showed LLT with low toxicity and high efficiency. LLTis a more drug-like compound comparing with the parent compound. METHODS Male DBA/1 mice were immunized at the tail base with 125 ( g of Bovine type II collagen emulsified in CFA containing Mycobacterium tuberculosis and boosted with collagen emulsified in IFA 3 weeks later. The severity of arthritis was graded visually and expressed using the arthritic index. The arthritic score of each mouse was obtained by scoring each limb as severity 0 to 4 and taken the sum of the scores of the four limbs.展开更多
AIM To evaluate the validity of LLT in prevention and therapy of acute graft versus host disease and to clarify the underlying mechanisms. LLT is a new compound derived from triptolide, which is the major immunosuppre...AIM To evaluate the validity of LLT in prevention and therapy of acute graft versus host disease and to clarify the underlying mechanisms. LLT is a new compound derived from triptolide, which is the major immunosuppressive fraction of Tripterygium wilfordii Hook. F (TWHF). Studies in vitro and in vivo demonstrated that LLT had potent immunosuppressive activities. Here we tested the immunosuppressive effects of LLT in murine allogeneic bone marrow transplantation (BMT) and investigated the mechanisms underlying its suppressive effects. METHODS LLT was administered to recipients in BALB/cA→C57BL/6 murine BMT model, in which donor and recipient differ at major and minor histocompatibility antigens.Survival and weight change were recorded in recipients after alloBMT. Spleen size, chimerism and splenic T cell subpopulation were analysed by using flow cytometry.展开更多
Two new flavonoids, (2S)-4′ ,5-dihydroxyl-6,7-di-O-β-D-glu-copyranosylflavanone (1) and 6-hydroxykaempferol 6,7-di-O-β-D-glucopyranoside (2), were isolated fromCarthamus tinctorius . Their structures were elucidate...Two new flavonoids, (2S)-4′ ,5-dihydroxyl-6,7-di-O-β-D-glu-copyranosylflavanone (1) and 6-hydroxykaempferol 6,7-di-O-β-D-glucopyranoside (2), were isolated fromCarthamus tinctorius . Their structures were elucidated by spectroscopic means including 2D NMR,ESIMS and CD.展开更多
Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese Nati...Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese National Human Genome Center at Shanghai, by using bio- informatics analysis. Comparative analysis of the two- component regulatory systems in S. epidermidis and that of S. aureus and Bacillus subtilis shows that these systems may regulate some important biological functions, e.g. growth, biofilm formation, and expression of virulence factors in S. epidermidis. Two conserved domains, i.e. HATPase_c and REC domains, are found in all 16 pairs of two-component proteins. Homologous modelling analysis indicates that there are 4 similar HATPase_c domain structures of histidine kinases and 13 similar REC domain structures of response regulators, and there is one AMP-PNP binding pocket in the HATPase_c domain and three active aspartate residues in the REC domain. Preliminary experiment reveals that the bioinfor- matics analysis of the conserved domain structures in the two-component regulatory systems in S. epidermidis may provide useful information for discovery of potential drug target.展开更多
This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages.A comprehensive kinetics of cytokine productio...This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages.A comprehensive kinetics of cytokine production was performed in murine peritoneal macrophages recovered from Balb/c mice at various time during the course of an intraperitoneal injection with thioglycollate (TG).The expression of cell surface molecules such as MHC-Ⅰ,MHC-Ⅱ,B7-1 and B7-2 of these macrophages were also determined by flow cytometry.The present findings of our research suggested that the population of activated macrophages and the activation of macrophages (including cytokines production and expression of cell surface functional molecules) were strictly controlled during inflammation process.This is one of the important mechanisms to retain the host homeostasis.Cellular & Molecular Immunology.2004;1(1):57-62.展开更多
文摘A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace levels; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.
文摘Huperzine V, a new Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and the absolute stereochemistry was determined by X-ray crystallographic analysis.
文摘Huperzine W, a novel 14 carbons Lycopodium alkaloid, was isolated from the whole plant of Huperzia serrata, and its stucture was determined by spectroscopic analysis.
文摘Further phytochemical investigation of the ethyl acetate extract of the stem of Daphniphyllum oldhamii afforded a new phenolic glucoside, named oldhamioside 1, together with eleven known compounds, koaburaside 2, betulin, 28-hydroxyl-3-lupenone, pineresinol, syringaresinol, 4-O-methyl- cedrusin, narigenin, eriodictyol, apigenin, loureirin C, and asperuloside. The structures of new and known compounds were characterized by detailed spectroscopic analysis and comparison of their spectral data with reported values.
文摘A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s camphor sultam as chiral auxiliary.
文摘A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the anti-osteoporosis agents.
文摘The stereoselective synthesis of (2R, 4R)-2-N-tert-butyloxycarbonyl amino-4, 5-epoxido- valeric acid methyl ester 8, which is the key intermediate for the synthesis of (2S, 2R) -3-trans-nitrocyclopropyl-alanine, was first accomplished.
文摘Two new steroids, nanjiols D and E, were isolated from the soft coral Nephthea bayeri. Their structures were characterized by spectroscopic methods and comparison with known compounds.
基金supported by the National Natural Science Foundation of China (No. 30123005) and Bicoll Biotechnology Shanghai Co Ltd. Authors thank Professor Qiong Xin ZHONG (Department of Biological Science Hainan Normal University) for collecting Stemona parviflora
文摘A novel alkaloid was isolated from the stems and leaves of Stemona parviflora Wright. Based on the spectral methods, its structure was elucidated as parvineostemonine.
文摘AIM To evaluate the therapeutic effects of LLT on the type II collagen-induced arthritis in DBA/1 mice. LLT is a new compound derived from triptolide. LLT had been demonstrated in vitro and in vivo for its immunosuppressive activities, and our results showed LLT with low toxicity and high efficiency. LLTis a more drug-like compound comparing with the parent compound. METHODS Male DBA/1 mice were immunized at the tail base with 125 ( g of Bovine type II collagen emulsified in CFA containing Mycobacterium tuberculosis and boosted with collagen emulsified in IFA 3 weeks later. The severity of arthritis was graded visually and expressed using the arthritic index. The arthritic score of each mouse was obtained by scoring each limb as severity 0 to 4 and taken the sum of the scores of the four limbs.
文摘AIM To evaluate the validity of LLT in prevention and therapy of acute graft versus host disease and to clarify the underlying mechanisms. LLT is a new compound derived from triptolide, which is the major immunosuppressive fraction of Tripterygium wilfordii Hook. F (TWHF). Studies in vitro and in vivo demonstrated that LLT had potent immunosuppressive activities. Here we tested the immunosuppressive effects of LLT in murine allogeneic bone marrow transplantation (BMT) and investigated the mechanisms underlying its suppressive effects. METHODS LLT was administered to recipients in BALB/cA→C57BL/6 murine BMT model, in which donor and recipient differ at major and minor histocompatibility antigens.Survival and weight change were recorded in recipients after alloBMT. Spleen size, chimerism and splenic T cell subpopulation were analysed by using flow cytometry.
文摘Two new flavonoids, (2S)-4′ ,5-dihydroxyl-6,7-di-O-β-D-glu-copyranosylflavanone (1) and 6-hydroxykaempferol 6,7-di-O-β-D-glucopyranoside (2), were isolated fromCarthamus tinctorius . Their structures were elucidated by spectroscopic means including 2D NMR,ESIMS and CD.
文摘Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese National Human Genome Center at Shanghai, by using bio- informatics analysis. Comparative analysis of the two- component regulatory systems in S. epidermidis and that of S. aureus and Bacillus subtilis shows that these systems may regulate some important biological functions, e.g. growth, biofilm formation, and expression of virulence factors in S. epidermidis. Two conserved domains, i.e. HATPase_c and REC domains, are found in all 16 pairs of two-component proteins. Homologous modelling analysis indicates that there are 4 similar HATPase_c domain structures of histidine kinases and 13 similar REC domain structures of response regulators, and there is one AMP-PNP binding pocket in the HATPase_c domain and three active aspartate residues in the REC domain. Preliminary experiment reveals that the bioinfor- matics analysis of the conserved domain structures in the two-component regulatory systems in S. epidermidis may provide useful information for discovery of potential drug target.
基金supported by the National Natural Science Foundation of China,No.A30171086the Knowledge Innovation Program of Chinese Academy of Sciences and the number is KSCX2-SW-202
文摘This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages.A comprehensive kinetics of cytokine production was performed in murine peritoneal macrophages recovered from Balb/c mice at various time during the course of an intraperitoneal injection with thioglycollate (TG).The expression of cell surface molecules such as MHC-Ⅰ,MHC-Ⅱ,B7-1 and B7-2 of these macrophages were also determined by flow cytometry.The present findings of our research suggested that the population of activated macrophages and the activation of macrophages (including cytokines production and expression of cell surface functional molecules) were strictly controlled during inflammation process.This is one of the important mechanisms to retain the host homeostasis.Cellular & Molecular Immunology.2004;1(1):57-62.
