An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were c...An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer （pH 7.8） containing different concentrations of NaC1 is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purit lysozyme from chicken egg white.展开更多
The origin, age and evolution of the Precambrian metamorphic basement of southern China provide useful insights into early crustal development. Here, we present new laser ablation-inductively coupled plasma-mass spect...The origin, age and evolution of the Precambrian metamorphic basement of southern China provide useful insights into early crustal development. Here, we present new laser ablation-inductively coupled plasma-mass spectrometry （LA-ICP-MS） U-Pb age data for detrital zircons from five samples of the Precambrian metamorphic basement of the Xiangshan uranium orefield. Two of these samples, from the northern Xiangshan volcanic basin, yielded a total of 140 U-Pb ages that cluster within the Neoproterozoic （773-963 Ma; 79.3% of data points）, with the rest being scattered through the Paleoproterozoic and Mesoproterozoic, along with a single Archean age. These ages indicate that this basement material is associated with the Cathaysia Block. In comparison, the 172 concordant ages from the other three samples from the southern part of the Xiangshan volcanic basin cluster within the Neoproterozoic （767-944 Ma; 59.8%） as well as the Proterozoic （37.8%） and the Archean （2502-2712 Ma; 14.5%）. These samples are also free of zircons with Grenvillian ages, indicating that these units are associated with the southeastern Yangtze Block. Combining these data with the geochemistry of these units, which suggests that the metamorphosed sedimentary rocks within the northern and southern parts of the Xiangshan basin have a common component from a magmatic island arc that formed during the early Neoproterozoic, we infer that the basin was located along the boundary between the Cathaysian and Yangtze blocks. In addition, the zircons within the samples from the southern and northern parts of the Xiangshan basin show different pre-Neoproterozoic （963 Ma） age populations but similar post- Neoproterozoic zircon populations, indicating that the amalgamation of the Cathaysian and Yangtze blocks occurred after the Neoproterozoic （960 Ma）, with magmatism peaking at 830 Ma and rifting starting at -770 Ma, leading to the subsequent deposition （from bottom to top） of the Shenshan, Kuli, and Shangshi formations.展开更多
Acoustic Emission(AE)waveforms contain information on microscopic structural features that can be related with damage of coal rock masses.In this paper,the Hilbert-Huang transform(HHT)method is used to obtain detailed...Acoustic Emission(AE)waveforms contain information on microscopic structural features that can be related with damage of coal rock masses.In this paper,the Hilbert-Huang transform(HHT)method is used to obtain detailed structural characteristics of coal rock masses associated with damage,at different loading stages,from the analyses of the characteristics of AE waveforms.The results show that the HHT method can be used to decompose the target waveform into multiple intrinsic mode function(IMF)components,with the energy mainly concentrated in the c1−c4 IMF components,where the c1 component has the highest frequency and the largest amount of energy.As the loading continues,the proportion of energy occupied by the low-frequency IMF component shows an increasing trend.In the initial compaction stage,the Hilbert marginal spectrum is mainly concentrated in the low frequency range of 0−40 kHz.The plastic deformation stage is associated to energy accumulation in the frequency range of 0−25 kHz and 200−350 kHz,while the instability damage stage is mainly concentrated in the frequency range of 0−25 kHz.At 20 kHz,the instability damage reaches its maximum value.There is a relatively clear instantaneous energy peak at each stage,albeit being more distinct at the beginning and at the end of the compaction phase.Since the effective duration of the waveform is short,its resulting energy is small,and so there is a relatively high value from the instantaneous energy peak.The waveform lasts a relatively long time after the peak that coincides with failure,which is the period where the waveform reaches its maximum energy level.The Hilbert three-dimensional energy spectrum is generally zero in the region where the real energy is zero.In addition,its energy spectrum is intermittent rather than continuous.It is therefore consistent with the characteristics of the several dynamic ranges mentioned above,and it indicates more clearly the low-frequency energy concentration in the critical stage of instability failure.This study well reflects the response law of geophysical signals in the process of coal rock instability and failure,providing a basis for monitoring coal rock dynamic disasters.展开更多
Aconite is a valuable drug and also a toxic material, which can be used only after detoxification processing. Although traditional processing methods can achieve detoxification effect as desired, there are some obviou...Aconite is a valuable drug and also a toxic material, which can be used only after detoxification processing. Although traditional processing methods can achieve detoxification effect as desired, there are some obvious drawbacks, including a significant loss of alkaloids and poor quality consistency. It is thus necessary to develop a new detoxification approach. In the present study, we designed a novel one-step detoxification approach by quickly drying fresh-cut aconite particles. In order to evaluate the technical advantages, the contents of mesaconitine, aconitine, hypaconitine, benzoylmesaconine, benzoylaconine, benzoylhypaconine, neoline, fuziline, songorine, and talatisamine were determined using HPLC and UHPLC/Q-TOF-MS. Multivariate analysis methods, such as Clustering analysis and Principle component analysis, were applied to determine the quality differences between samples. Our results showed that traditional processes could reduce toxicity as desired, but also led to more than 85.2% alkaloids loss. However, our novel one-step method was capable of achieving virtually the same detoxification effect, with only an approximately 30% alkaloids loss. Cluster analysis and Principal component analysis analyses suggested that Shengfupian and the novel products were significantly different from various traditional products. Acute toxicity testing showed that the novel products achieved a good detoxification effect, with its maximum tolerated dose being equivalent to 20 times of adult dosage. And cardiac effect testing also showed that the activity of the novel products was stronger than that of traditional products. Moreover, particles specification greatly improved the quality consistency of the novel products, which was immensely superior to the traditional products. These results would help guide the rational optimization of aconite processing technologies, providing better drugs for clinical treatment.展开更多
In this work, a series of MIL-101-SO3H（x） polymeric materials were prepared and further used for the first time as efficient heterogeneous catalysts for the conversion of fructose-based carbohydrates into 5-ethoxyme...In this work, a series of MIL-101-SO3H（x） polymeric materials were prepared and further used for the first time as efficient heterogeneous catalysts for the conversion of fructose-based carbohydrates into 5-ethoxymethylfurfural（EMF） in a renewable mixed solvent system consisting of ethanol and tetrahydrofuran（THF）. The influence of –SO3H content on the acidity as well as on the catalytic activity of the porous coordination polymers in EMF production was also studied. High EMF yields of 67.7% and 54.2% could be successively obtained from fructose and inulin in the presence of MIL-101-SO;H（100） at 130 °C for 15 h.The catalyst could be reused for five times without significant loss of its activity and the recovery process was facile and simple. This work provides a new platform by application of porous coordination polymers（PCPs） for the production of the potential liquid fuel molecule EMF from biomass in a sustainable solvent system.展开更多
Plant virus disease is one of the major causes of biological disasters in agriculture worldwide. Given the complexity of transmission media and plant disease infection mechanisms, the prevention and control of plant v...Plant virus disease is one of the major causes of biological disasters in agriculture worldwide. Given the complexity of transmission media and plant disease infection mechanisms, the prevention and control of plant viral diseases is a great challenge, and an efficient green pesticide is urgently needed. For this reason, when developing candidate drug leads to regulate plant viruses, pesticide experts have focused on characteristics such as low pesticide resistance, eco-friendliness, and novel mechanism. Researchers have also theoretically investigated the molecular targets of viruses infecting agricultural crops. Antiviral screening models have been constructed based on these molecular targets, and the mechanisms of commercial drugs and high-activity compounds have been extensively investigated. After screening, some compounds have been applied in the field and found to have good commercial prospects; these drugs may be used to create new green antiviral pesticides to control plant viruses. This paper reviews the screening, mode of action, development and application of recently used plant-based antiviral agents.展开更多
Voluminous platinum-group mineral(PGM)inclusions including erlichmanite(Os,Ru)S2,laurite(Ru,Os)S2,and irarsite(Ir,Os,Ru,Rh)AsS,as well as native osmium Os(Ir)and inclusions of base metal sulphides(BMS),including mille...Voluminous platinum-group mineral(PGM)inclusions including erlichmanite(Os,Ru)S2,laurite(Ru,Os)S2,and irarsite(Ir,Os,Ru,Rh)AsS,as well as native osmium Os(Ir)and inclusions of base metal sulphides(BMS),including millerite(NiS),heazlewoodite(NiaS2),covellite(CuS)and digenite(Cu3S2),accompanied by native iron,have been identified in chromitites of the Zedang ophiolite,Xizang.The PGMs occur as both inclusions in magnesiochromite grains and as small interstitial granules between them;most are less than 10~m in size and vary in shape from euhedral to anhedral.They occur either as single or composite(biphase or polyphase)grains composed solely of PGM,or PGM associated with silicate grains.Os-,Ir-,and Ru-rich PGMs are the common species and Pt-,Pd-,and Rh-rich varieties have not been identified.Sulfur fugacity and temperature appear to be the main factors that controlled the PGE mineralogy during crystallization of the host chromitite in the upper mantle.If the activity of chalcogenides(such as S,and As)is low,PGE clusters will remain suspended in the silicate melt until they can coalesce to form alloys.Under appropriate conditions of fS2 and fO2,PGE alloys might react with the melt to form sulfides-sulfarsenides.Thus,we suggest that the Os,Ir and Ru metallic clusters and alloys in the Zedang chromitites crystallized first under high temperature and low fS2,followed by crystallization of sulphides of the laurite-erlichmanite,solid-solution series as the magma cooled and fS2 increased.The abundance of primary BMS in the chromitites suggests that fS2 reached relatively high values during the final stages of magnesiochromite crystallization.The diversity of the PGE minerals,in combination with differences in the petrological characteristics of the magnesiochromites,suggest different degrees of partial melting,perhaps at different depths in the mantle.The estimated parental magma composition suggests formation in a suprasubduction zone environment,perhaps in a forearc.展开更多
The coal mining process is afected by various water sources such as groundwater and coal seam water injection.Understanding the dynamic mechanical parameters of water-immersed coal is helpful for coalmine safe product...The coal mining process is afected by various water sources such as groundwater and coal seam water injection.Understanding the dynamic mechanical parameters of water-immersed coal is helpful for coalmine safe production.The impact compression tests were performed on coal with diferent moisture contents by using theϕ50 mm Split Hopkinson Pressure Bar(SHPB)experimental system,and the dynamic characteristics and energy loss laws of water-immersed coal with diferent compositions and water contents were analyzed.Through analysis and discussion,it is found that:(1)When the moisture content of the coal sample is 0%,30%,60%,the stress,strain rate and energy frst increase and then decrease with time.(2)When the moisture content of the coal sample increases from 30%to 60%,the stress“plateau”of the coal sample becomes more obvious,resulting in an increase in the compressive stress stage and a decrease in the expansion stress stage.(3)The increase of moisture content of the coal sample will afect its impact deformation and failure mode.When the moisture content is 60%,the incident rod end and the transmission rod end of the coal sample will have obvious compression failure,and the middle part of the coal sample will also experience expansion and deformation.(4)The coal composition ratio suitable for the coal immersion softening impact experiment is optimized.展开更多
Research on water-splitting electrocatalysts is crucial to establishing a solution to the energy crisis.Herein,we report a facile bottom-up strategy for the preparation of high performance supported electrocatalysts f...Research on water-splitting electrocatalysts is crucial to establishing a solution to the energy crisis.Herein,we report a facile bottom-up strategy for the preparation of high performance supported electrocatalysts for overall water-splitting reaction via a rationally designed defect covalent triazine frameworks(CTFs)support.Specifically,defect CTFs are obtained via binary-precursor polymerization,followed by loading Ru nanoparticles(Ru/D-CTFs-900)with high HER performance at a current density of 10 mA cm-2.The overpotential is only 17 mV.Calcination of the resultant Ru–RuO2/D-CTFs-300 in air,produces excellent OER performance with 190 mV overpotential(at 10 mA cm-2).Furthermore,overall water splitting measurements reveal the potential of 1.47 V,which is better than the majority of the reported Ru-based catalysts.Moreover,density functional theory calculation results show that excellent electrocatalytic properties are attributed to the synergistic effect of Ru nanoparticles and carbon support.展开更多
Oxygen evolution from water driven by electrocatalysis or photocatalysis poses a significant challenge as it requires the use of efficient electro-/photo-catalysts to drive the four-electron oxygen evolution reaction(...Oxygen evolution from water driven by electrocatalysis or photocatalysis poses a significant challenge as it requires the use of efficient electro-/photo-catalysts to drive the four-electron oxygen evolution reaction(OER).Herein,we report the development of an effective strategy for the in situ chemical transformation of Fe-based bimetallic MIL-88 metal-organic frameworks(MOFs)into corresponding bimetallic hydroxides,which are composed of amorphous ultrasmall nanoparticles and afford an abundance of catalytically active sites.Optimized MOF-derived NiFe-OH-0.75 catalyst coated on glassy carbon electrodes achieved a current density of 10 mA cm^(-2)in the electrocatalytic OER with a small overpotential of 270 mV,which could be decreased to 235 mV when loading the catalysts on a nickel foam substrate.Moreover,these MOF-derived Fe-based bimetallic hydroxides can be used as efficient cocatalysts when combined with suitable photosensitizers for photocatalytic water oxidation.展开更多
To better retain useful weak low-frequency magnetotelluric(MT)signals with strong interference during MT data processing,we propose a SVM-CEEMDWT based MT data signal-noise separation method,which extracts the weak MT...To better retain useful weak low-frequency magnetotelluric(MT)signals with strong interference during MT data processing,we propose a SVM-CEEMDWT based MT data signal-noise separation method,which extracts the weak MT signal affected by strong interference.First,the approximate entropy,fuzzy entropy,sample entropy,and Lempel-Ziv(LZ)complexity are extracted from the magnetotelluric data.Then,four robust parameters are used as the inputs to the support vector machine(SVM)to train the sample library and build a model based on the different complexity of signals.Based on this model,we can only consider time series with strong interference when using the complementary ensemble empirical mode decomposition(CEEMD)and wavelet threshold(WT)for noise suppression.Simulation results suggest that the SVM based on the robust parameters can distinguish the time periods with strong interference well before noise suppression.Compared with the CEEMD WT,the proposed SVM-CEEMDWT method retains more low-frequency low-variability information,and the apparent resistivity curve is smoother and more continuous.Moreover,the results better reflect the deep electrical structure in the field.展开更多
Ligand modification of Ni-based catalysts by coordination of dicyandiamide to Ni metal leads to enhanced selectivity for the selective hydrogenation of halonitroaromatics.The selectivity of above 99.9%to aromatic halo...Ligand modification of Ni-based catalysts by coordination of dicyandiamide to Ni metal leads to enhanced selectivity for the selective hydrogenation of halonitroaromatics.The selectivity of above 99.9%to aromatic haloamines can be achieved at the conversion of 100%.The results of H_2-TPD and FT-IR experiments show that Ni^-H~＋ species possessing the properties of Lewis acid site on the surface of Raney Ni could be responsible for the hydrodehalogenation.When Raney Ni was treated by dicyandiamide,Ni^-H~＋ species interacted with N atom from the dicyandiamide.This interaction was stable even at reaction temperature,which reduced the possibility to form the intermediate state of Ar-Cl...H~＋Ni^-.And then C-Cl bond could not be polarized and activated.The hvdrodechlorination process was suppressed effectively.展开更多
Photo-responsive mechanical actuator is a class of stimuli-responsive materials transferring light to mechanical energy through macroscopic transformation.To fabricate photo-responsive mechanical actuator,soft polymer...Photo-responsive mechanical actuator is a class of stimuli-responsive materials transferring light to mechanical energy through macroscopic transformation.To fabricate photo-responsive mechanical actuator,soft polymeric materials crosslinked with functional bridging structures are desired.Supramolecular interaction is a relatively common way to fabricate crosslinked materials due to its excellent self-assembly performance.And azobenzene and derivatives are ideal candidates of photo-responsive materials because of the unique photo-induced trans-cis isomerization.Here,a new kind of crosslinked materials based on supramolecular interaction between 4,4'-dihydroxyazobenzene and chitosan is reported.Under 355 nm irradiation,the macroscopic bending of polymeric materials occurs rapidly due to the photo-isomerization of 4,4-dihydroxyazobenzene.Meanwhile,the photo-responsive mechanical actuator can also lift weight which is up to 200 times that of the actuator itself,and convert energy from light to mechanical work efficiently.This report suggests a new kind of photo-responsive actuator based on supramolecular interaction and may be helpful to contribute a theoretical basis to the design and synthesis of photo-responsive mechanical actuator suitable for large-scale manufacturing industrialization in future.展开更多
Based on the reversible host-vip inclusion/exclusion of cyclodextrin-functionalized graphene oxide(GO-CD) and azobenzene-terminated polyhedral oligomeric silsesquioxane(Azo-POSS), a novel kind of light-responsive na...Based on the reversible host-vip inclusion/exclusion of cyclodextrin-functionalized graphene oxide(GO-CD) and azobenzene-terminated polyhedral oligomeric silsesquioxane(Azo-POSS), a novel kind of light-responsive nanocomposites GO-POSS was developed under mild condition. ~1H NMR, ET-IR,TG, TEM and UV-vis spectroscopy were conducted to characterize the chemical composition and photoresponsive performance of obtained GO-POSS nanocomposites. The results demonstrated that nanocagestructured POSS and nanosheet GO components in GO-POSS exhibited pronounced supra molecular assembly/disassembly behavior upon UV/vis irradiation. Moreover, GO-POSS nanocomposites showed good water dispersity and had remarkable impact on oxygen permeability of conventional PVA-coated films under varied light irradiation conditions, which would be valuable for developing smart gas barrier materials in packaging.展开更多
Numerous Bt rice lines expressing Cry protein derived from Bacillus thuringiensis Berliner （Bt） have been developed since 1989. However, the potential risks posed by Bt rice on non-target organisms still remain deba...Numerous Bt rice lines expressing Cry protein derived from Bacillus thuringiensis Berliner （Bt） have been developed since 1989. However, the potential risks posed by Bt rice on non-target organisms still remain debate. The white-backed planthopper （WBPH）, Sogatella furcifera （Horváth）, is one of the most economically important insect pests of rice in Asian countries and also one of the main non-target herbivores of transgenic rice. In the current study, impacts of transgenic cry1Ab/vip3H＋epsps rice （G6H1） with both insect and herbicide resistance on WBPH were evaluated to ascertain whether this transgenic rice line had potential risks for this sap-sucking pest under laboratory and ifeld conditions. The laboratory results showed that no signiifcant difference in egg developmental duration, nymphal survival rate and female fecundity was found for WBPH between G6H1 and its non-transgenic isoline （XS110）. However, the development duration of nymphs was signiifcantly shorter and female longevity signiifcantly longer when WBPH fed on G6H1 by comparison with those on its control. To verify the results found in laboratory, a 3-yr ifeld trial was conducted to monitor WBPH population using both the vacuum-suction machine and beat plate methods. Although the seasonal density of WBPH nymphs and total density of nymphs and adults were not signiifcantly affected by transgenic rice regardless of the sampling methods, the seasonal density of WBPH adults in transgenic rice plots was slightly lower than that in the control when using the vacuum-suction machine. Based on these results both from laboratory and ifeld, it is clear that our tested transgenic rice line will not lead higher population of WBPH. However, long-term ifeld experiments to monitor the population dynamics of WPBH at large scale need to be conducted to conifrm the present conclusions in future.展开更多
This paper proposes a decoherence-immune scheme for generating highly entangled states for two atoms trapped in a cavity. The scheme is based on two resonant atom-cavity interactions. Conditional upon the detection of...This paper proposes a decoherence-immune scheme for generating highly entangled states for two atoms trapped in a cavity. The scheme is based on two resonant atom-cavity interactions. Conditional upon the detection of no photon, the two atoms may exchange an excitation via the first resonant interaction, which leads to entanglement. Due to the loss of the excitation, the two atoms are in a mixed entangled state. With the help of an auxiliary ground state not coupled to the cavity mode, the state related to the excitation loss is eliminated by the detection of a photon resulting from the second resonant interaction. Thus, the fidelity of entanglement is almost not affected by the decoherence.展开更多
We report herein a new fluorescent probe for the selective recognition and determination of dodine among 20 different pesticides.This fluorescent probe was assembled through host-vip complexation between cucurbit[10...We report herein a new fluorescent probe for the selective recognition and determination of dodine among 20 different pesticides.This fluorescent probe was assembled through host-vip complexation between cucurbit[10]uril(Q[10]) and aminopropyl-1-pyrenebutanamide(PBA) and is designated as PBA@Q[10].Addition of dodine to PBA@Q[10] results in a dramatic enhancement of fluorescence intensity at 390 nm,accompanied by fluorescence quenching at 488 nm.On this basis,the detection limit is 6.78 × 10^(-7) mol/L.The response mechanism is a competitive interaction:dodine occupies the cavity of Q[10] and forces PBA to leave.展开更多
The discovery process of pesticides is confronting more and more difficult obstacles,including the rising costs of materials and labor,which are costly and time-consuming.Pesticide informatics brings an opportunity fo...The discovery process of pesticides is confronting more and more difficult obstacles,including the rising costs of materials and labor,which are costly and time-consuming.Pesticide informatics brings an opportunity for structure-based molecular design and optimization,which could improve the efficiency of pesticides discovery.However,there are still some problems in using informatics methods to improve the efficiency of pesticide discovery.Here,we provide a summary of databases and tools used in pesticide informatics,including those for target information search,virtual screening(VS),scaffold optimization,and pesticide-likeness assessment.Moreover,some successful cases of hit compound optimization utilizing the aforementioned approaches were dissected.We hope this review can guide researchers choose appropriate techniques to increase the effectiveness of hit compounds identification and optimization.展开更多
We propose a scheme for the deterministic generation of qutrit entanglement for two atoms trapped in an optical cavity. Taking advantage of the adiabatic passage, the operation is immune to atomic spontaneous emission...We propose a scheme for the deterministic generation of qutrit entanglement for two atoms trapped in an optical cavity. Taking advantage of the adiabatic passage, the operation is immune to atomic spontaneous emission as the atomic excited states are never populated; under certain conditions, the probability that the cavity is excited is negligible. We also study the influences of the dissipation due to the atomic spontaneous emission and cavity decay.展开更多
Water-based architectural heat insulation coatings were studied to overcome the drawbacks of conventional inorganic silicate heat insulation coatings. The heat insulation coatings were prepared with the method of mech...Water-based architectural heat insulation coatings were studied to overcome the drawbacks of conventional inorganic silicate heat insulation coatings. The heat insulation coatings were prepared with the method of mechanical agitation when the mixed organic polymer emulsions were used as binder of the coatings and the mixed heat insulating aggregates were applied as powder, and some assistants were also added. Water temperature difference in the plastic container, which was coated with heat insulation coatings, represented the heat-insulating property of the coatings. The influences of components of mixed polymer emulsion, mass ratio of polymer emulsion to powder, particle size of heat insulating aggregates, added amount of air entraining admixture and the match of thickeners on the properties of the coatings were studied. The experimental results show that the heat insulation coatings with good finishing, heat-insulation property and artificial weathering can be prepared when the binder is composed of 66.92% styrene-acrylic emulsion, 16.59% elastic emulsion and 16.49% silicone-acrylic emulsion, the mass ratio of polymer emulsion to powder is 0.45, the particle size of heat insulating aggregates is in the rang of 200 and 250 mesh size, the added amount of sericite is 15%, and the added amount of air entraining admixture is in the range of 1.0% and 1.5% and the thickeners are the mixtures of ASE-60 and RM-5000.展开更多
基金Supported by the National lqatural Science Foundation of China （21036005, 20876145）, the Science and Technology Cooperation Project between China-Europe Country＇s Governments from the Ministry of Science and Technology of China （1017） and the Natural Science Foundation of Zhejiang Provincial （Y4080326）.
文摘An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer （pH 7.8） containing different concentrations of NaC1 is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purit lysozyme from chicken egg white.
基金financially supported by the National Natural Science Foundation of China(Grant No.41602069 and 41572185)the Fundamental Science on Radioactive Geology and Exploration Technology Laboratory(Grant No.RGET1402)+1 种基金the Natural Science Foundation of Jiangxi Province(Grant No.20171BAB213026)Science and technology research projectfrom the Education Department of Jiangxi Province(Grant No.GJJ150554)
文摘The origin, age and evolution of the Precambrian metamorphic basement of southern China provide useful insights into early crustal development. Here, we present new laser ablation-inductively coupled plasma-mass spectrometry （LA-ICP-MS） U-Pb age data for detrital zircons from five samples of the Precambrian metamorphic basement of the Xiangshan uranium orefield. Two of these samples, from the northern Xiangshan volcanic basin, yielded a total of 140 U-Pb ages that cluster within the Neoproterozoic （773-963 Ma; 79.3% of data points）, with the rest being scattered through the Paleoproterozoic and Mesoproterozoic, along with a single Archean age. These ages indicate that this basement material is associated with the Cathaysia Block. In comparison, the 172 concordant ages from the other three samples from the southern part of the Xiangshan volcanic basin cluster within the Neoproterozoic （767-944 Ma; 59.8%） as well as the Proterozoic （37.8%） and the Archean （2502-2712 Ma; 14.5%）. These samples are also free of zircons with Grenvillian ages, indicating that these units are associated with the southeastern Yangtze Block. Combining these data with the geochemistry of these units, which suggests that the metamorphosed sedimentary rocks within the northern and southern parts of the Xiangshan basin have a common component from a magmatic island arc that formed during the early Neoproterozoic, we infer that the basin was located along the boundary between the Cathaysian and Yangtze blocks. In addition, the zircons within the samples from the southern and northern parts of the Xiangshan basin show different pre-Neoproterozoic （963 Ma） age populations but similar post- Neoproterozoic zircon populations, indicating that the amalgamation of the Cathaysian and Yangtze blocks occurred after the Neoproterozoic （960 Ma）, with magmatism peaking at 830 Ma and rifting starting at -770 Ma, leading to the subsequent deposition （from bottom to top） of the Shenshan, Kuli, and Shangshi formations.
基金Projects(51904167, 51474134, 51774194) supported by the National Natural Science Foundation of ChinaProject(SKLCRSM19KF008) supported by the Research Fund of the State Key Laboratory of Coal Resources and Safe Mining,CUMT,China+5 种基金Project(cstc2019jcyj-bsh0041) supported by the Natural Science Foundation of Chongqing,ChinaProject(2011DA105287-BH201903) supported by the Postdoctoral ScienceFunded by State Key Laboratory of Coal Mine Disaster Dynamics and Control,ChinaProject(2019SDZY034-2) supported by the Key R&D plan of Shandong Province,ChinaProject(2020M670781) supported by the China Postdoctoral Science FoundationProject supported by the Taishan Scholars ProjectProject supported by the Taishan Scholar Talent Team Support Plan for Advantaged&Unique Discipline Areas,China
文摘Acoustic Emission(AE)waveforms contain information on microscopic structural features that can be related with damage of coal rock masses.In this paper,the Hilbert-Huang transform(HHT)method is used to obtain detailed structural characteristics of coal rock masses associated with damage,at different loading stages,from the analyses of the characteristics of AE waveforms.The results show that the HHT method can be used to decompose the target waveform into multiple intrinsic mode function(IMF)components,with the energy mainly concentrated in the c1−c4 IMF components,where the c1 component has the highest frequency and the largest amount of energy.As the loading continues,the proportion of energy occupied by the low-frequency IMF component shows an increasing trend.In the initial compaction stage,the Hilbert marginal spectrum is mainly concentrated in the low frequency range of 0−40 kHz.The plastic deformation stage is associated to energy accumulation in the frequency range of 0−25 kHz and 200−350 kHz,while the instability damage stage is mainly concentrated in the frequency range of 0−25 kHz.At 20 kHz,the instability damage reaches its maximum value.There is a relatively clear instantaneous energy peak at each stage,albeit being more distinct at the beginning and at the end of the compaction phase.Since the effective duration of the waveform is short,its resulting energy is small,and so there is a relatively high value from the instantaneous energy peak.The waveform lasts a relatively long time after the peak that coincides with failure,which is the period where the waveform reaches its maximum energy level.The Hilbert three-dimensional energy spectrum is generally zero in the region where the real energy is zero.In addition,its energy spectrum is intermittent rather than continuous.It is therefore consistent with the characteristics of the several dynamic ranges mentioned above,and it indicates more clearly the low-frequency energy concentration in the critical stage of instability failure.This study well reflects the response law of geophysical signals in the process of coal rock instability and failure,providing a basis for monitoring coal rock dynamic disasters.
基金supported by National Nature Science Fundation of China(Nos.81274026 and 81403115)
文摘Aconite is a valuable drug and also a toxic material, which can be used only after detoxification processing. Although traditional processing methods can achieve detoxification effect as desired, there are some obvious drawbacks, including a significant loss of alkaloids and poor quality consistency. It is thus necessary to develop a new detoxification approach. In the present study, we designed a novel one-step detoxification approach by quickly drying fresh-cut aconite particles. In order to evaluate the technical advantages, the contents of mesaconitine, aconitine, hypaconitine, benzoylmesaconine, benzoylaconine, benzoylhypaconine, neoline, fuziline, songorine, and talatisamine were determined using HPLC and UHPLC/Q-TOF-MS. Multivariate analysis methods, such as Clustering analysis and Principle component analysis, were applied to determine the quality differences between samples. Our results showed that traditional processes could reduce toxicity as desired, but also led to more than 85.2% alkaloids loss. However, our novel one-step method was capable of achieving virtually the same detoxification effect, with only an approximately 30% alkaloids loss. Cluster analysis and Principal component analysis analyses suggested that Shengfupian and the novel products were significantly different from various traditional products. Acute toxicity testing showed that the novel products achieved a good detoxification effect, with its maximum tolerated dose being equivalent to 20 times of adult dosage. And cardiac effect testing also showed that the activity of the novel products was stronger than that of traditional products. Moreover, particles specification greatly improved the quality consistency of the novel products, which was immensely superior to the traditional products. These results would help guide the rational optimization of aconite processing technologies, providing better drugs for clinical treatment.
基金financially supported by the Natural Science Foundation of China(no.21576059)the Key Technologies R&D Program(no.2011BAE06B02)+1 种基金the International Science&Technology Cooperation Program of China(2010DFB60840)the Science and Technology Project of Guizhou Province(nos.[2012]6012 and[2011]3016)
文摘In this work, a series of MIL-101-SO3H（x） polymeric materials were prepared and further used for the first time as efficient heterogeneous catalysts for the conversion of fructose-based carbohydrates into 5-ethoxymethylfurfural（EMF） in a renewable mixed solvent system consisting of ethanol and tetrahydrofuran（THF）. The influence of –SO3H content on the acidity as well as on the catalytic activity of the porous coordination polymers in EMF production was also studied. High EMF yields of 67.7% and 54.2% could be successively obtained from fructose and inulin in the presence of MIL-101-SO;H（100） at 130 °C for 15 h.The catalyst could be reused for five times without significant loss of its activity and the recovery process was facile and simple. This work provides a new platform by application of porous coordination polymers（PCPs） for the production of the potential liquid fuel molecule EMF from biomass in a sustainable solvent system.
基金supported by the National Natural Science Foundation of China (21132003, 31460460, 21502032 and 21732002)the Subsidy Project for Outstanding Key Laboratory of Guizhou Province in China (20154004)+1 种基金the Provincial University Cooperation Plan of Guizhou Province in China (20147001)the Collaborative Innovation Center for Natural Products and Biological Drugs of Yunnan, China
文摘Plant virus disease is one of the major causes of biological disasters in agriculture worldwide. Given the complexity of transmission media and plant disease infection mechanisms, the prevention and control of plant viral diseases is a great challenge, and an efficient green pesticide is urgently needed. For this reason, when developing candidate drug leads to regulate plant viruses, pesticide experts have focused on characteristics such as low pesticide resistance, eco-friendliness, and novel mechanism. Researchers have also theoretically investigated the molecular targets of viruses infecting agricultural crops. Antiviral screening models have been constructed based on these molecular targets, and the mechanisms of commercial drugs and high-activity compounds have been extensively investigated. After screening, some compounds have been applied in the field and found to have good commercial prospects; these drugs may be used to create new green antiviral pesticides to control plant viruses. This paper reviews the screening, mode of action, development and application of recently used plant-based antiviral agents.
基金financially supported by the National Natural Science Foundation of China(Grant No.41262002,40930313,41302052,41502062)National Industry Special Projects(Sino Probe-05-02)+2 种基金China Bureau of Geological Survey Projects(DD20160023-01)IGCP-649Institute of Geology,Chinese Academy of Geological Science(J1526)
文摘Voluminous platinum-group mineral(PGM)inclusions including erlichmanite(Os,Ru)S2,laurite(Ru,Os)S2,and irarsite(Ir,Os,Ru,Rh)AsS,as well as native osmium Os(Ir)and inclusions of base metal sulphides(BMS),including millerite(NiS),heazlewoodite(NiaS2),covellite(CuS)and digenite(Cu3S2),accompanied by native iron,have been identified in chromitites of the Zedang ophiolite,Xizang.The PGMs occur as both inclusions in magnesiochromite grains and as small interstitial granules between them;most are less than 10~m in size and vary in shape from euhedral to anhedral.They occur either as single or composite(biphase or polyphase)grains composed solely of PGM,or PGM associated with silicate grains.Os-,Ir-,and Ru-rich PGMs are the common species and Pt-,Pd-,and Rh-rich varieties have not been identified.Sulfur fugacity and temperature appear to be the main factors that controlled the PGE mineralogy during crystallization of the host chromitite in the upper mantle.If the activity of chalcogenides(such as S,and As)is low,PGE clusters will remain suspended in the silicate melt until they can coalesce to form alloys.Under appropriate conditions of fS2 and fO2,PGE alloys might react with the melt to form sulfides-sulfarsenides.Thus,we suggest that the Os,Ir and Ru metallic clusters and alloys in the Zedang chromitites crystallized first under high temperature and low fS2,followed by crystallization of sulphides of the laurite-erlichmanite,solid-solution series as the magma cooled and fS2 increased.The abundance of primary BMS in the chromitites suggests that fS2 reached relatively high values during the final stages of magnesiochromite crystallization.The diversity of the PGE minerals,in combination with differences in the petrological characteristics of the magnesiochromites,suggest different degrees of partial melting,perhaps at different depths in the mantle.The estimated parental magma composition suggests formation in a suprasubduction zone environment,perhaps in a forearc.
基金This research was funded by the National Natural Science Foundation of China(51974176,51934004)ShandongProvince Natural Science Foundation of Outstanding Youth Fund(ZR2020JQ22)+1 种基金Shandong Province Colleges and Universities Youth Innovation and Technology Support Program(2019KJH006)Taishan Scholars Project(TS20190935).
文摘The coal mining process is afected by various water sources such as groundwater and coal seam water injection.Understanding the dynamic mechanical parameters of water-immersed coal is helpful for coalmine safe production.The impact compression tests were performed on coal with diferent moisture contents by using theϕ50 mm Split Hopkinson Pressure Bar(SHPB)experimental system,and the dynamic characteristics and energy loss laws of water-immersed coal with diferent compositions and water contents were analyzed.Through analysis and discussion,it is found that:(1)When the moisture content of the coal sample is 0%,30%,60%,the stress,strain rate and energy frst increase and then decrease with time.(2)When the moisture content of the coal sample increases from 30%to 60%,the stress“plateau”of the coal sample becomes more obvious,resulting in an increase in the compressive stress stage and a decrease in the expansion stress stage.(3)The increase of moisture content of the coal sample will afect its impact deformation and failure mode.When the moisture content is 60%,the incident rod end and the transmission rod end of the coal sample will have obvious compression failure,and the middle part of the coal sample will also experience expansion and deformation.(4)The coal composition ratio suitable for the coal immersion softening impact experiment is optimized.
基金National Natural Science Foundation of China(Grant nos.21671172,21625604,21706229,21776251)Zhejiang Provincial Natural Science Foundation of China(Grant no.LR19B010001)open fund of State Key Laboratory of Structural Chemistry(No.20170036)。
文摘Research on water-splitting electrocatalysts is crucial to establishing a solution to the energy crisis.Herein,we report a facile bottom-up strategy for the preparation of high performance supported electrocatalysts for overall water-splitting reaction via a rationally designed defect covalent triazine frameworks(CTFs)support.Specifically,defect CTFs are obtained via binary-precursor polymerization,followed by loading Ru nanoparticles(Ru/D-CTFs-900)with high HER performance at a current density of 10 mA cm-2.The overpotential is only 17 mV.Calcination of the resultant Ru–RuO2/D-CTFs-300 in air,produces excellent OER performance with 190 mV overpotential(at 10 mA cm-2).Furthermore,overall water splitting measurements reveal the potential of 1.47 V,which is better than the majority of the reported Ru-based catalysts.Moreover,density functional theory calculation results show that excellent electrocatalytic properties are attributed to the synergistic effect of Ru nanoparticles and carbon support.
文摘Oxygen evolution from water driven by electrocatalysis or photocatalysis poses a significant challenge as it requires the use of efficient electro-/photo-catalysts to drive the four-electron oxygen evolution reaction(OER).Herein,we report the development of an effective strategy for the in situ chemical transformation of Fe-based bimetallic MIL-88 metal-organic frameworks(MOFs)into corresponding bimetallic hydroxides,which are composed of amorphous ultrasmall nanoparticles and afford an abundance of catalytically active sites.Optimized MOF-derived NiFe-OH-0.75 catalyst coated on glassy carbon electrodes achieved a current density of 10 mA cm^(-2)in the electrocatalytic OER with a small overpotential of 270 mV,which could be decreased to 235 mV when loading the catalysts on a nickel foam substrate.Moreover,these MOF-derived Fe-based bimetallic hydroxides can be used as efficient cocatalysts when combined with suitable photosensitizers for photocatalytic water oxidation.
基金funded by the National Key R&D Program of China(No.2018YFC0603202)the National Natural Science Foundation of China(No.41404111)+1 种基金Natural Science Foundation of Hunan Province(No.2018JJ2258)Hunan Provincial Science and Technology Project Foundation(No.2018TP1018)
文摘To better retain useful weak low-frequency magnetotelluric(MT)signals with strong interference during MT data processing,we propose a SVM-CEEMDWT based MT data signal-noise separation method,which extracts the weak MT signal affected by strong interference.First,the approximate entropy,fuzzy entropy,sample entropy,and Lempel-Ziv(LZ)complexity are extracted from the magnetotelluric data.Then,four robust parameters are used as the inputs to the support vector machine(SVM)to train the sample library and build a model based on the different complexity of signals.Based on this model,we can only consider time series with strong interference when using the complementary ensemble empirical mode decomposition(CEEMD)and wavelet threshold(WT)for noise suppression.Simulation results suggest that the SVM based on the robust parameters can distinguish the time periods with strong interference well before noise suppression.Compared with the CEEMD WT,the proposed SVM-CEEMDWT method retains more low-frequency low-variability information,and the apparent resistivity curve is smoother and more continuous.Moreover,the results better reflect the deep electrical structure in the field.
基金the National Basic Research Program of China(973 Program)(No 2011CB710800)the National Natural Science Foundation of China(No20976164)
文摘Ligand modification of Ni-based catalysts by coordination of dicyandiamide to Ni metal leads to enhanced selectivity for the selective hydrogenation of halonitroaromatics.The selectivity of above 99.9%to aromatic haloamines can be achieved at the conversion of 100%.The results of H_2-TPD and FT-IR experiments show that Ni^-H~＋ species possessing the properties of Lewis acid site on the surface of Raney Ni could be responsible for the hydrodehalogenation.When Raney Ni was treated by dicyandiamide,Ni^-H~＋ species interacted with N atom from the dicyandiamide.This interaction was stable even at reaction temperature,which reduced the possibility to form the intermediate state of Ar-Cl...H~＋Ni^-.And then C-Cl bond could not be polarized and activated.The hvdrodechlorination process was suppressed effectively.
基金supported by the National Natural Science Foundation of China(Nos.51703112,51673106)Natural Science Foundation of Shandong Province(Nos.ZR2017BEM040,ZR2016BQ28)+1 种基金Open Fund of the State Key Laboratory of Luminescent Materials and Devices(No.2017-skllmd-10,South China University of Technology)China Postdoctoral Science Foundation(No.2017M622152).
文摘Photo-responsive mechanical actuator is a class of stimuli-responsive materials transferring light to mechanical energy through macroscopic transformation.To fabricate photo-responsive mechanical actuator,soft polymeric materials crosslinked with functional bridging structures are desired.Supramolecular interaction is a relatively common way to fabricate crosslinked materials due to its excellent self-assembly performance.And azobenzene and derivatives are ideal candidates of photo-responsive materials because of the unique photo-induced trans-cis isomerization.Here,a new kind of crosslinked materials based on supramolecular interaction between 4,4'-dihydroxyazobenzene and chitosan is reported.Under 355 nm irradiation,the macroscopic bending of polymeric materials occurs rapidly due to the photo-isomerization of 4,4-dihydroxyazobenzene.Meanwhile,the photo-responsive mechanical actuator can also lift weight which is up to 200 times that of the actuator itself,and convert energy from light to mechanical work efficiently.This report suggests a new kind of photo-responsive actuator based on supramolecular interaction and may be helpful to contribute a theoretical basis to the design and synthesis of photo-responsive mechanical actuator suitable for large-scale manufacturing industrialization in future.
基金the Natural Science Foundation of China (No.51503181)for financial support
文摘Based on the reversible host-vip inclusion/exclusion of cyclodextrin-functionalized graphene oxide(GO-CD) and azobenzene-terminated polyhedral oligomeric silsesquioxane(Azo-POSS), a novel kind of light-responsive nanocomposites GO-POSS was developed under mild condition. ~1H NMR, ET-IR,TG, TEM and UV-vis spectroscopy were conducted to characterize the chemical composition and photoresponsive performance of obtained GO-POSS nanocomposites. The results demonstrated that nanocagestructured POSS and nanosheet GO components in GO-POSS exhibited pronounced supra molecular assembly/disassembly behavior upon UV/vis irradiation. Moreover, GO-POSS nanocomposites showed good water dispersity and had remarkable impact on oxygen permeability of conventional PVA-coated films under varied light irradiation conditions, which would be valuable for developing smart gas barrier materials in packaging.
基金provided by the Special Research Projects for Developing Transgenic Plants, China (2013ZX08011-001)China National Science Fund for Innovative Research Group of Biological Control (31021003)the National Basic Research Progarm of China (973 Program, 2007CB109202)
文摘Numerous Bt rice lines expressing Cry protein derived from Bacillus thuringiensis Berliner （Bt） have been developed since 1989. However, the potential risks posed by Bt rice on non-target organisms still remain debate. The white-backed planthopper （WBPH）, Sogatella furcifera （Horváth）, is one of the most economically important insect pests of rice in Asian countries and also one of the main non-target herbivores of transgenic rice. In the current study, impacts of transgenic cry1Ab/vip3H＋epsps rice （G6H1） with both insect and herbicide resistance on WBPH were evaluated to ascertain whether this transgenic rice line had potential risks for this sap-sucking pest under laboratory and ifeld conditions. The laboratory results showed that no signiifcant difference in egg developmental duration, nymphal survival rate and female fecundity was found for WBPH between G6H1 and its non-transgenic isoline （XS110）. However, the development duration of nymphs was signiifcantly shorter and female longevity signiifcantly longer when WBPH fed on G6H1 by comparison with those on its control. To verify the results found in laboratory, a 3-yr ifeld trial was conducted to monitor WBPH population using both the vacuum-suction machine and beat plate methods. Although the seasonal density of WBPH nymphs and total density of nymphs and adults were not signiifcantly affected by transgenic rice regardless of the sampling methods, the seasonal density of WBPH adults in transgenic rice plots was slightly lower than that in the control when using the vacuum-suction machine. Based on these results both from laboratory and ifeld, it is clear that our tested transgenic rice line will not lead higher population of WBPH. However, long-term ifeld experiments to monitor the population dynamics of WPBH at large scale need to be conducted to conifrm the present conclusions in future.
基金Project supported by funds from the State Key Laboratory Breeding Base of Photocatalysis,Fuzhou University
文摘This paper proposes a decoherence-immune scheme for generating highly entangled states for two atoms trapped in a cavity. The scheme is based on two resonant atom-cavity interactions. Conditional upon the detection of no photon, the two atoms may exchange an excitation via the first resonant interaction, which leads to entanglement. Due to the loss of the excitation, the two atoms are in a mixed entangled state. With the help of an auxiliary ground state not coupled to the cavity mode, the state related to the excitation loss is eliminated by the detection of a photon resulting from the second resonant interaction. Thus, the fidelity of entanglement is almost not affected by the decoherence.
基金the National Natural Science Foundation of China(No.21861011)the Major Program for Creative Research Groups ofGuizhou Provincial Education Department(No.2017-028)+1 种基金the Innovation Program for High-level Talents of Guizhou Province(No.2016-5657)the Science and Technology Fund of Guizhou Province(Nos.2016-1030,2018-5781)are gratefully acknowledged for financial support。
文摘We report herein a new fluorescent probe for the selective recognition and determination of dodine among 20 different pesticides.This fluorescent probe was assembled through host-vip complexation between cucurbit[10]uril(Q[10]) and aminopropyl-1-pyrenebutanamide(PBA) and is designated as PBA@Q[10].Addition of dodine to PBA@Q[10] results in a dramatic enhancement of fluorescence intensity at 390 nm,accompanied by fluorescence quenching at 488 nm.On this basis,the detection limit is 6.78 × 10^(-7) mol/L.The response mechanism is a competitive interaction:dodine occupies the cavity of Q[10] and forces PBA to leave.
基金supported by the National Natural Science Foundation of China(32125033 and 31960548)Program of Introducing Talents of Discipline to Universities of China(111 Program,D20023).
文摘The discovery process of pesticides is confronting more and more difficult obstacles,including the rising costs of materials and labor,which are costly and time-consuming.Pesticide informatics brings an opportunity for structure-based molecular design and optimization,which could improve the efficiency of pesticides discovery.However,there are still some problems in using informatics methods to improve the efficiency of pesticide discovery.Here,we provide a summary of databases and tools used in pesticide informatics,including those for target information search,virtual screening(VS),scaffold optimization,and pesticide-likeness assessment.Moreover,some successful cases of hit compound optimization utilizing the aforementioned approaches were dissected.We hope this review can guide researchers choose appropriate techniques to increase the effectiveness of hit compounds identification and optimization.
基金supported by the National Natural Science Foundation of China (Grant Nos.10674025 and 10974028)the Fujian Natural Science Foundation of China (Grant No.2009J06002)+2 种基金the Doctoral Foundation of the Ministry of Education of China(Grant Nos.20070386002 and 20093514110009)the Funds from State Key Laboratory Breeding Base of Photocatalysis,Fuzhou Universitythe Funds from the Education Department of Fujian Province of China (Grant No.JB08010)
文摘We propose a scheme for the deterministic generation of qutrit entanglement for two atoms trapped in an optical cavity. Taking advantage of the adiabatic passage, the operation is immune to atomic spontaneous emission as the atomic excited states are never populated; under certain conditions, the probability that the cavity is excited is negligible. We also study the influences of the dissipation due to the atomic spontaneous emission and cavity decay.
基金Funded by the Scientific Research Foundation for Postdoctor(20061023)
文摘Water-based architectural heat insulation coatings were studied to overcome the drawbacks of conventional inorganic silicate heat insulation coatings. The heat insulation coatings were prepared with the method of mechanical agitation when the mixed organic polymer emulsions were used as binder of the coatings and the mixed heat insulating aggregates were applied as powder, and some assistants were also added. Water temperature difference in the plastic container, which was coated with heat insulation coatings, represented the heat-insulating property of the coatings. The influences of components of mixed polymer emulsion, mass ratio of polymer emulsion to powder, particle size of heat insulating aggregates, added amount of air entraining admixture and the match of thickeners on the properties of the coatings were studied. The experimental results show that the heat insulation coatings with good finishing, heat-insulation property and artificial weathering can be prepared when the binder is composed of 66.92% styrene-acrylic emulsion, 16.59% elastic emulsion and 16.49% silicone-acrylic emulsion, the mass ratio of polymer emulsion to powder is 0.45, the particle size of heat insulating aggregates is in the rang of 200 and 250 mesh size, the added amount of sericite is 15%, and the added amount of air entraining admixture is in the range of 1.0% and 1.5% and the thickeners are the mixtures of ASE-60 and RM-5000.
