Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups ...Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups using a random number table method:3,3’-iminodipropionitrile(IDPN)group,control group,acupuncture group,and tiapride group,with 9 mice in each group.In the IDPN group,acupuncture group,and tiapride group,mice received daily intraperitoneal injections of IDPN(300 mg/kg body weight)for 7 consecutive days to induce stereotyped behaviors.Subsequently,in the acupuncture intervention group,standardized acupuncture treatment was administered for 14 consecutive days to IDPN-induced TS model mice.The selected acupoints included Baihui(DU 20),Yintang(DU 29),Waiguan(SJ 5),and Zulinqi(GB 41).In the tiapride group,mice were administered tiapride(50 mg/kg body weight)via oral gavage daily for 14 consecutive days.The control group,IDPN group,and acupuncture group received the same volume of saline orally for 14 consecutive days.Stereotypic behaviors were quantified through behavioral assessments.Neurotransmitter levels,including dopamine(DA),glutamate(Glu),and aspartate(ASP)in striatal tissue were measured using enzyme-linked immunosorbent assay.Dopamine transporter(DAT)expression levels were additionally quantified through quantitative polymerase chain reaction(qPCR).Gut microbial composition was analyzed through 16S ribosomal RNA gene sequencing,while metabolic profiling was conducted using liquid chromatography-mass spectrometry(LC-MS).展开更多
基金Supported by the National Natural Science Foundation of China(No.U23A20507 and No.82074522)FU Wen-bin Famous Guangdong TCM Physician Inheritance Studio of Guangdong Provincial TCM Administration(Guangdong TCM Office Letter[2020]No.1)+1 种基金Guangdong Provincial Hospital of Situ Ling lnheritance Studio of Lingnan Acupuncture School(TCM Second Hospital[2013]No.233)Natural Science Foundation of Guangdong Province(No.2024A1515010291)。
文摘Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups using a random number table method:3,3’-iminodipropionitrile(IDPN)group,control group,acupuncture group,and tiapride group,with 9 mice in each group.In the IDPN group,acupuncture group,and tiapride group,mice received daily intraperitoneal injections of IDPN(300 mg/kg body weight)for 7 consecutive days to induce stereotyped behaviors.Subsequently,in the acupuncture intervention group,standardized acupuncture treatment was administered for 14 consecutive days to IDPN-induced TS model mice.The selected acupoints included Baihui(DU 20),Yintang(DU 29),Waiguan(SJ 5),and Zulinqi(GB 41).In the tiapride group,mice were administered tiapride(50 mg/kg body weight)via oral gavage daily for 14 consecutive days.The control group,IDPN group,and acupuncture group received the same volume of saline orally for 14 consecutive days.Stereotypic behaviors were quantified through behavioral assessments.Neurotransmitter levels,including dopamine(DA),glutamate(Glu),and aspartate(ASP)in striatal tissue were measured using enzyme-linked immunosorbent assay.Dopamine transporter(DAT)expression levels were additionally quantified through quantitative polymerase chain reaction(qPCR).Gut microbial composition was analyzed through 16S ribosomal RNA gene sequencing,while metabolic profiling was conducted using liquid chromatography-mass spectrometry(LC-MS).
