Background While maternal psychological stress during mid-to-late pregnancy has been linked to offspring allergies,the impact of early pregnancy distress remains unclear.This study investigates the association between...Background While maternal psychological stress during mid-to-late pregnancy has been linked to offspring allergies,the impact of early pregnancy distress remains unclear.This study investigates the association between maternal depressive and anxiety symptoms in early pregnancy and allergic diseases in offspring.Methods Based on a birth cohort of 5263 children,antenatal depressive and anxiety symptoms in early pregnancy were assessed via the Patient Health Questionnaire and Generalized Anxiety Disorder Questionnaire,respectively.Allergic outcomes,including asthma,atopic dermatitis(AD),and allergic rhinitis(AR),were evaluated via structured questionnaires.Relative risks(RRs)with 95%confidence intervals(CIs)were estimated via generalized linear models,whereas restricted cubic splines were used to explore linear and non-linear associations between maternal distress and allergic outcomes.Results Maternal depressive symptoms in early pregnancy were associated with an increased risk of AD[adjusted RR(95%CI)=1.15(1.03–1.29)]and AR[1.52(1.29–1.79)].Maternal anxiety symptoms in early pregnancy were associated with increased risks of AD[1.11(1.02–1.21),mild anxiety]and AR[1.33(1.04–1.68),moderate to severe anxiety].Dose‒response analyses revealed graded relationships between distress severity and allergic outcomes.In the joint analysis,comorbid depression and anxiety in early pregnancy were associated with an increased risk of AD[1.15(1.05–1.26)]and AR[1.42(1.23–1.63)].Subgroup analysis revealed a greater risk of asthma for boys born to mothers with mild anxiety[1.95(1.20–3.15)]but not for girls.Conclusion Maternal distress in early pregnancy is associated with an increased risk of allergic diseases in offspring during toddlerhood.展开更多
Background and Objectives:Previous studies have reported there were associations between ovarian function and dietary factors,metabolic factors and gut microbiota.However,it is unclear whether causal associations exis...Background and Objectives:Previous studies have reported there were associations between ovarian function and dietary factors,metabolic factors and gut microbiota.However,it is unclear whether causal associations exist.We aimed to explore the causal relationship of these factors with risk of primary ovarian failure(POF).Methods and Study Design:Two-sample Mendelian randomization(MR)analysis was performed to genetically predict the causal effects of dietary and metabolic factors and gut microbiota on POF.The inverse variance weighted(IVW)method was used as the primary statistical method.A series of sensitivity analyses,including weighted median,MR-Egger,simple mode,weighted mode methods,and leave-one-out analysis,were conducted to assess the robustness of the MR analysis results.Results:IVW analysis revealed that cigarettes smoked per day,coffee intake and cooked vegetable intake were not causally correlated with POF at the genetic level.However,POF were associated with fresh fruit intake,BMI,Eubacterium(hallii group),Eubacterium(ventriosum group),Ad lercreutzia,Intestinibacter,Lachnospiraceae(UCG008),and Terrisporobacter.These findings were robust ac cording to extensive sensitivity analyses.Conclusions:This study identified several dietary factors,metabolic factors and gut microbiota taxa that may be causally implicated in POF,potentially offering new therapeutic tar gets.展开更多
Objective:Frozen-thawed embryo transfer(FET)is widely used inin vitro fertilization(IVF)clinics but is associated with an increased risk of several pregnancy complications,including large-for-gestational age and place...Objective:Frozen-thawed embryo transfer(FET)is widely used inin vitro fertilization(IVF)clinics but is associated with an increased risk of several pregnancy complications,including large-for-gestational age and placenta-related diseases.However,the effects of FET on placentation remain unclear.Therefore,we used single-cell RNA-sequencing(scRNA-seq)technology to investigate the impact of FET on placental gene expression in different subtypes of trophoblasts.Methods:A mouse model of IVF and FET was constructed to collect placenta tissues.scRNA-seq was performed on placentas from two dams undergoing IVF-embryo transfer and two dams undergoing IVF-FET.Differentially expressed gene(DEG)analyses were performed in different subtypes of trophoblasts.Identified DEGs were polymerase chain reaction(PCR)validated.Results:The fetal weights and placental efficiency were higher in the FET group than those in the IVF group at E18.5,with no significant difference in placental weights.Subsequently,55,406 placental cells were captured and annotated.Upregulated DEGs in the FET group in syncytiotrophoblasts(SynTs)and sinusoidal trophoblast giant cells(S-TGCs)within the placental labyrinth were enriched in pathways related to vascular development and oxidative stress,respectively.The expression of the imprinted geneIgf2 in SynTs,S-TGCs,and spongiotrophoblasts was significantly increased.In the junctional zone,FET upregulated the expression of prolactin genes such asPrl3b1 in glycogen trophoblasts(GlyTs)while the downregulated expression of GlyT genes following FET was associated with mesenchyme development.Conclusions:This study first identifies DEGs and enriched pathways in different subtypes of trophoblasts following FET.These genes and pathways may contribute to the increased placental efficiency and fetal weights.Future studies are required to confirm these results and further explore the key mechanisms in placental pathologies.展开更多
Antenatal depression is a significant contributor to maternal morbidity in the perinatal period[1].It has also been associated with an increased risk for premature delivery,decreased rates of breastfeeding initiation,...Antenatal depression is a significant contributor to maternal morbidity in the perinatal period[1].It has also been associated with an increased risk for premature delivery,decreased rates of breastfeeding initiation,and a broad range of adverse child out-comes,including emotional problems,externalizing difficulties,attachment issues,and poorer cognitive development[2-6]Depression in the antenatal period may persist postnatally and increase in severity further exacerbating the negative conse-quences.There are considerable geographical differences in ante-natal depression prevalence rates across China,ranging from 9.8%to 35.4%,which is likely due to variations in demographic,lifestyle,social,and economic factors[7,8].Heterogeneity in study design and data source has also resulted in these wide variations.Further,previous Chinese studies examining antenatal depression have been limited due to relatively small sample sizes,short study peri-ods,and a focus on only one specific geographical district.Limited prevalence data in China suggest that a large-scale multi-center study is warranted.展开更多
基金supported by National Key Research and Development Program of China(2022YFC2703500)National Natural Science Foundation of China(8211101588,82088102 and 82171686)+10 种基金Program of Shanghai Academic Research Leader(20XD1424100)Natural Science Foundation of Shanghai(20ZR1463100)Clinical research program of Shanghai Municipal Health Commission(202340222)Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)Clinical Research Plan of Shanghai Shenkang Hospital Development Center(SHDC2023CRD001 and SHDC2020CR1008A)STI2030-Major Projects(2021ZD0200700)CAMS Innovation Fund for Medical Sciences(2019-I2M-5–064)Shanghai Clinical Research Center for Gynecological Diseases(22MC1940200)Shanghai Urogenital System Diseases Research Center(2022ZZ01012)Key Discipline Construction Project(2023–2025)of Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai(GWVI-11.1–35)the specific research fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202311)and Shanghai Frontiers Science Research Base of Reproduction and Development.
文摘Background While maternal psychological stress during mid-to-late pregnancy has been linked to offspring allergies,the impact of early pregnancy distress remains unclear.This study investigates the association between maternal depressive and anxiety symptoms in early pregnancy and allergic diseases in offspring.Methods Based on a birth cohort of 5263 children,antenatal depressive and anxiety symptoms in early pregnancy were assessed via the Patient Health Questionnaire and Generalized Anxiety Disorder Questionnaire,respectively.Allergic outcomes,including asthma,atopic dermatitis(AD),and allergic rhinitis(AR),were evaluated via structured questionnaires.Relative risks(RRs)with 95%confidence intervals(CIs)were estimated via generalized linear models,whereas restricted cubic splines were used to explore linear and non-linear associations between maternal distress and allergic outcomes.Results Maternal depressive symptoms in early pregnancy were associated with an increased risk of AD[adjusted RR(95%CI)=1.15(1.03–1.29)]and AR[1.52(1.29–1.79)].Maternal anxiety symptoms in early pregnancy were associated with increased risks of AD[1.11(1.02–1.21),mild anxiety]and AR[1.33(1.04–1.68),moderate to severe anxiety].Dose‒response analyses revealed graded relationships between distress severity and allergic outcomes.In the joint analysis,comorbid depression and anxiety in early pregnancy were associated with an increased risk of AD[1.15(1.05–1.26)]and AR[1.42(1.23–1.63)].Subgroup analysis revealed a greater risk of asthma for boys born to mothers with mild anxiety[1.95(1.20–3.15)]but not for girls.Conclusion Maternal distress in early pregnancy is associated with an increased risk of allergic diseases in offspring during toddlerhood.
基金funded by National Key Research and De velopment Program of China(No.2022YFC2703803,No.2022YFC2703001,No.2021YFC2700603)National Natural Science Foundation of China(No.82088102,No.82171613,No.82171688)+5 种基金CAMS Innovation Fund for Medical Sciences(2019-I2M-5-064)Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)Key Discipline Construction Project(2023-2025)of Three-Year Initiative Plan for Strengthening Public Health System Con struction in Shanghai(GWVI-11.1-35)Shanghai Clinical Re search Center for Gynecological Diseases(22MC1940200)Shanghai Urogenital System Diseases Research Center(2022ZZ01012)Shanghai Frontiers Science Research Center of Reproduction and Development,Zhejiang Province College Student Science and Technology Innovation Program(Xinmiao Plan)(2023R401210).
文摘Background and Objectives:Previous studies have reported there were associations between ovarian function and dietary factors,metabolic factors and gut microbiota.However,it is unclear whether causal associations exist.We aimed to explore the causal relationship of these factors with risk of primary ovarian failure(POF).Methods and Study Design:Two-sample Mendelian randomization(MR)analysis was performed to genetically predict the causal effects of dietary and metabolic factors and gut microbiota on POF.The inverse variance weighted(IVW)method was used as the primary statistical method.A series of sensitivity analyses,including weighted median,MR-Egger,simple mode,weighted mode methods,and leave-one-out analysis,were conducted to assess the robustness of the MR analysis results.Results:IVW analysis revealed that cigarettes smoked per day,coffee intake and cooked vegetable intake were not causally correlated with POF at the genetic level.However,POF were associated with fresh fruit intake,BMI,Eubacterium(hallii group),Eubacterium(ventriosum group),Ad lercreutzia,Intestinibacter,Lachnospiraceae(UCG008),and Terrisporobacter.These findings were robust ac cording to extensive sensitivity analyses.Conclusions:This study identified several dietary factors,metabolic factors and gut microbiota taxa that may be causally implicated in POF,potentially offering new therapeutic tar gets.
基金National Key Research and Development Program of China(2021YFC2700700,2022YFC2703500)National Natural Science Foundation of China(82171686)+4 种基金Clinical Research Program of Shanghai Municipal Health Commission(202340222)Natural Science Foundation of Shanghai(20ZR1463100)Clinical Research Plan of Shanghai Shenkang Hospital Development Center(SHDC2023CRD001)Shanghai Clinical Research Center for Gynecological Diseases(22MC1940200)Shanghai Urogenital System Diseases Research Center(2022ZZ01012)。
文摘Objective:Frozen-thawed embryo transfer(FET)is widely used inin vitro fertilization(IVF)clinics but is associated with an increased risk of several pregnancy complications,including large-for-gestational age and placenta-related diseases.However,the effects of FET on placentation remain unclear.Therefore,we used single-cell RNA-sequencing(scRNA-seq)technology to investigate the impact of FET on placental gene expression in different subtypes of trophoblasts.Methods:A mouse model of IVF and FET was constructed to collect placenta tissues.scRNA-seq was performed on placentas from two dams undergoing IVF-embryo transfer and two dams undergoing IVF-FET.Differentially expressed gene(DEG)analyses were performed in different subtypes of trophoblasts.Identified DEGs were polymerase chain reaction(PCR)validated.Results:The fetal weights and placental efficiency were higher in the FET group than those in the IVF group at E18.5,with no significant difference in placental weights.Subsequently,55,406 placental cells were captured and annotated.Upregulated DEGs in the FET group in syncytiotrophoblasts(SynTs)and sinusoidal trophoblast giant cells(S-TGCs)within the placental labyrinth were enriched in pathways related to vascular development and oxidative stress,respectively.The expression of the imprinted geneIgf2 in SynTs,S-TGCs,and spongiotrophoblasts was significantly increased.In the junctional zone,FET upregulated the expression of prolactin genes such asPrl3b1 in glycogen trophoblasts(GlyTs)while the downregulated expression of GlyT genes following FET was associated with mesenchyme development.Conclusions:This study first identifies DEGs and enriched pathways in different subtypes of trophoblasts following FET.These genes and pathways may contribute to the increased placental efficiency and fetal weights.Future studies are required to confirm these results and further explore the key mechanisms in placental pathologies.
基金supported by the STI2030-Major Projects(2021ZD0200700)the National Key Research and Development Program of China(2021YFC2701600 and 2022YFC2703500)+10 种基金the National Natural Science Foundation of China(82088102 and 82301719)Science and Technology Innovation Fund of Shanghai Jiao Tong University(YG2020YQ29)Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)Shanghai Municipal Commission of Health(20224Y0085 and 202340222)Open Fund Project of Guangdong Academy of Medical Sciences(YKY-KF202202)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-064)Shanghai Clinical Research Center for Gynecological Diseases(22MC1940200)Shanghai Urogenital Sys-tem Diseases Research Center(2022ZZ01012)Key Discipline Con-struction Project(2023-2025)of Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai(GWVI-11.1-35 and GWVI-11.2-YQ29)Shanghai Sailing Program(22YF1453100)Shanghai Frontiers Science Research Base of Reproduction and Development.
文摘Antenatal depression is a significant contributor to maternal morbidity in the perinatal period[1].It has also been associated with an increased risk for premature delivery,decreased rates of breastfeeding initiation,and a broad range of adverse child out-comes,including emotional problems,externalizing difficulties,attachment issues,and poorer cognitive development[2-6]Depression in the antenatal period may persist postnatally and increase in severity further exacerbating the negative conse-quences.There are considerable geographical differences in ante-natal depression prevalence rates across China,ranging from 9.8%to 35.4%,which is likely due to variations in demographic,lifestyle,social,and economic factors[7,8].Heterogeneity in study design and data source has also resulted in these wide variations.Further,previous Chinese studies examining antenatal depression have been limited due to relatively small sample sizes,short study peri-ods,and a focus on only one specific geographical district.Limited prevalence data in China suggest that a large-scale multi-center study is warranted.
