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Recent advances on micro-polluted water remediation by full-scale constructed wetlands:Pollutant removal performance,key influencing factors,and enhancing strategies
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作者 Qiang Ning Peihao Yan +4 位作者 Lingyan Zhao Zhiyi Lin Jian Zhang Zizhang Guo Haiming Wu 《Journal of Environmental Sciences》 2026年第1期565-576,共12页
The discharge of micro-polluted water from sources such as agricultural runoff,urban stormwater,and treated effluents presents significant challenges to aquatic ecosystems.Constructed wetlands(CWs)have gained recog-ni... The discharge of micro-polluted water from sources such as agricultural runoff,urban stormwater,and treated effluents presents significant challenges to aquatic ecosystems.Constructed wetlands(CWs)have gained recog-nition as an eco-friendly solution for removing pollutants from various wastewater sources and are increasingly applied for micro-polluted water treatment.By reviewing 78 full-scale CW studies from Web of Science,it is summarized that the ranges of ammonium nitrogen(NH4+-N)concentrations in runoff,wastewater treatment plant effluent and polluted river were 0.1–6.6,0.3–12.3,and 0.2–41.1 mg/L,respectively.The ranges of ni-trate nitrogen concentrations were 0.2–14.2,0–5.7,and 0–2.6 mg/L,respectively.Removal efficiencies of CWs for micro-polluted water varied by CW types.The total nitrogen removal efficiencies for subsurface-flow CWs,free-water surface-flow CWs,and hybrid CWs ranged from 27.4%to 66.5%,16.8%to 89.8%,and 19.4%to 88.2%,respectively.The NH4+-N removal efficiencies ranged from 34.2%to 73.6%,38.4%to 89.4%and 13.5%to 94.2%,respectively.Additionally,other factors influencing contaminant removal efficiency such as hydraulic retention time,vegetation types,redox micro-environment and influent water quality were evaluated.Based on these findings,two strategies for improving the purification performance of CWs were proposed:the selection of incorporating electron donor substrates and the optimization of operation parameters.This paper serves as a synthesis of information to guide future research and full-scale CW applications in micro-polluted water treatment. 展开更多
关键词 Constructed wetlands Micro-polluted water Nutrient removal Influencing factors Enhancing strategies
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Spatio-temporal analysis of human wellbeing and its coupling relationship with ecosystem services in Shandong province, China 被引量:5
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作者 WANG Chao WANG Xuan +5 位作者 WANG Yifan ZHAN Jinyan CHU Xi TENG Yanmin LIU Wei WANG Huihui 《Journal of Geographical Sciences》 SCIE CSCD 2023年第2期392-412,共21页
Rapid economic and social change promotes to improve human wellbeing(HW),but poses threats to ecosystems and the environment.Studying the coupling relationship between HW and ecosystem services(ES)is crucial for infor... Rapid economic and social change promotes to improve human wellbeing(HW),but poses threats to ecosystems and the environment.Studying the coupling relationship between HW and ecosystem services(ES)is crucial for informing high-quality development.Firstly,we built a comprehensive index system for HW assessment,and evaluated HW for 17 prefecture-level cities in Shandong province,China,from 2000 to 2018.Then,we quantified ES based on land use data.Finally,we assessed the coupling coordination degree and analyzed the relationships between HW and each type of ES value.The results were as follows:(1)HW values increased overall in Shandong,with the highest value in Jinan(0.8034)and the lowest value in Heze(0.4965)in 2018.(2)The total ES values for the 17 cities increased slightly.The ranking of 17 cities according to the ES value per unit area was different from the ranking according to the total ES value.(3)All 17 cities in Shandong were in the coordinated development phase after 2015,with increasing coupling coordination degrees.There were clear positive relationships between HW and ES.General and specific policy recommendations were proposed,providing scientific evidence and a reference for Shandong’s urban management and policy formulation. 展开更多
关键词 land use COUPLING human wellbeing ecosystem services SHANDONG
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Human Influence on Heavy Metal Distribution in the Upper Lake Nansi Sediments, Shandong Province, China 被引量:5
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作者 杨丽原 沈吉 +2 位作者 张祖陆 金章东 朱育新 《Chinese Journal Of Geochemistry》 EI CAS 2004年第2期177-185,共9页
Core and surface sediment samples were collected from three sub-lakes (Lake Nanyang, Lake Dushan and Lake Zhaoyang) in the Lake Nansi Basin, Shandong Province. In order to reveal the characteristics of spatial and his... Core and surface sediment samples were collected from three sub-lakes (Lake Nanyang, Lake Dushan and Lake Zhaoyang) in the Lake Nansi Basin, Shandong Province. In order to reveal the characteristics of spatial and historical distribution of heavy metals in different sub-lakes of the Upper Lake Nansi, heavy metal (As, Cr, Cu, Hg, K, Mn, Ni, Pb, Zn, Al, Fe, Ti and V) concentrations of sediment samples were investigated. Based on the activity of 137Cs in the sediments, the modern accumulation rate of Lake Nansi sediments is 3.5 mm/a. Our results show that the whole Upper Lake Nansi has been already polluted by heavy metals, among which Lake Nanyang has been polluted seriously by mercury, as well as by lead and arsenic, while Lake Dushan has been most seriously polluted by lead and arsenic. Historical variation of heavy metal (Cr, Cu, K, Ni, Zn, Al, Fe, Ti and V) concentrations shows an abrupt shift in 1962, resulting in a division of two periods: from 1957 to 1962 when metal enrichment increased with time, and from 1962 to 2000 when it decreased with time, while that of some anthropogenic elements such as Hg, Pb and Mn tend to increase toward the surface. However, the variation trend of As in the sediments is different from that of Hg, Pb and Mn, with its maximum value appearing in 1982. Since 1982 the concentrations of As have decreased due to the forbidden use of arsenite pesticides. This variation trend revealed changes in manner of human activity (coal combustion, waste discharges from both industries and urban sewage) within the catchment during different periods. 展开更多
关键词 重金属 人类活动 沉积学 水污染 山东
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Coal-based transformation of cities in Shandong Province,China 被引量:2
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作者 Zheng Xiang Xiyu Liu 《Chinese Journal of Population,Resources and Environment》 2015年第4期358-364,共7页
This article primarily discusses the issue of realizing sustainable development in Shandong Province,China through transformation.It compares the global energy structure to China's,as well as among regions and pro... This article primarily discusses the issue of realizing sustainable development in Shandong Province,China through transformation.It compares the global energy structure to China's,as well as among regions and provinces within China.The article takes six cities in Shandong Province as typical objectives,and then using principal component analysis it calculates their flexibility in regard to city shift and restructuring.The main purpose was to quantify the impact of economic activities triggered by the coal industry,a.k.a the energy economic nexus,and to assess the flexibility and sustainability in transformation among the observed coal-based cities. 展开更多
关键词 COAL transformation principal component analysis SUSTAINABILITY
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Eco-compensation of Wetlands in Yellow River Delta of Shandong Province,China 被引量:3
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作者 HAN Mei CUI Jinlong +2 位作者 HAO Zhen WANG Yi WANG Renqing 《Chinese Geographical Science》 SCIE CSCD 2012年第1期119-126,共8页
Wetlands play an important ecological role and provide many functions for people, yet wetlands are cur- rently decreasing and deteriorating. The ability to calculate an economic value for the loss of wetlands is becom... Wetlands play an important ecological role and provide many functions for people, yet wetlands are cur- rently decreasing and deteriorating. The ability to calculate an economic value for the loss of wetlands is becoming in- creasingly important for policy makers. In this study, remote sensing, field investigations, department visits, and other methods were used to survey wetland types, assess wetland area changes, and calculate wetland economic value. Mar- ket value loss and ecological ftmction value loss, caused by reduction of wetland area and environmental pollution were calculated using commonly accepted methods of market valuation, ecological valuation, environmental protection investment cost analysis, and outcome parameters. According to market value loss and ecological function value loss, preliminarily fund allocation for wetland and ecological compensation was calculated. This will provide an important reference for future Yellow River Delta eco-compensation studies. 展开更多
关键词 Yellow River Delta WETLAND eco-compensation compensation standard
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Evaluation of the Rural Human Settlement in Shandong Province 被引量:1
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作者 Xu Hong Shen Meiyu Chen Xiangxiang 《Chinese Journal of Population,Resources and Environment》 2012年第4期95-100,共6页
Taking Shandong Province as the research object,this paper uses the principal component analysis method to evaluate the status of the rural human settlement in Shandong Province.It establishes the evaluation index sys... Taking Shandong Province as the research object,this paper uses the principal component analysis method to evaluate the status of the rural human settlement in Shandong Province.It establishes the evaluation index system of the rural residential environment in Shandong Province,including living environment,economy,infrastructure,public service facilities,and ecological environment,in total five comprehensive index,and 20 secondary indexes.Through measurement and sorting of rural human environment development level of Shandong Province in 2010,the 17 cities are divided into-excellent,good,ordinary,poor-four development areas and are analyzed based on the restriction factor in the development of the region. 展开更多
关键词 rural human settlements comprehensive evaluation Shandong Province
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Influence Factors of the Regional Industrial Structure Evolution in Shandong Province of China against the Background of Low Carbon 被引量:1
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作者 LIU Hongyan WANG Chengxin +1 位作者 SHI Jialu CUI Xuegang 《Journal of Landscape Research》 2013年第Z3期13-15,共3页
Low-carbon development as the th eme of modern times is the premise to upgrade industrial structure. The authors adopt the PCA(Principle Component Analysis) to carry on a quantitative analysis about the factors that a... Low-carbon development as the th eme of modern times is the premise to upgrade industrial structure. The authors adopt the PCA(Principle Component Analysis) to carry on a quantitative analysis about the factors that affect the changing industrial structure in Shandong Province and then incorporate them into three principal components,namely,development-consumption,speed-structure and urbanization-technology. In other words,economic development is a direct factor while consumption demand and industrial upgrading are major driving forces. Moreover,urbanization accelerates the changes of industrial structure. 展开更多
关键词 PCA(Principle Component Analysis) Industrial structure Infl uential factors SHANDONG PROVINCE
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Sedimentary Characteristics,Ages,and Environmental Significance of Gravel Deposits and Loess in Shandong,Eastern China:Regional Response to Global Change Since the Last Glacial Period 被引量:1
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作者 WANG Min KONG Fanbiao +6 位作者 KONG Xianglun CHEN Haitao WANG Jiawei MIAO Xiaodong JIA Guangju HAN Mei XU Shujian 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第2期491-504,共14页
Investigation of rarely studied gravel layers found in the loess in Shandong Province,eastern China,reveals the fabric characteristics of two gravel layers(G1,G2)and the sedimentary characteristics of loess at the typ... Investigation of rarely studied gravel layers found in the loess in Shandong Province,eastern China,reveals the fabric characteristics of two gravel layers(G1,G2)and the sedimentary characteristics of loess at the typical and well-preserved Heiyu section(HY),where,to determine the paleoclimatic changes during Marine Isotope Stage 3a.Optically stimulated luminescence dates of the HY formation range from 0.26±0.02 ka to 39.00±2.00 ka.In addition,the ages of G1 and G2 were estimated using the Bayesian model to be 39.60-40.50 and 29.00-29.50 ka.G1 and G2 are mainly composed of fine and medium gravel,both of which were subangular to subrounded limestone,with gravel directions to NE and E.The average flow velocity,average depth,and flood peak flow of G1 are 1.10 m/s,0.49 m,and 37.04 m^(3)/s,respectively,calculated using the flow energy method,whereas those of G2 are 0.98 m/s,0.38 m,and 18.38 m^(3)/s,respectively.Analysis of climate proxy indices show that the sedimentary environment of the gravel and loess in HY might be a regional response to global change. 展开更多
关键词 PALEOENVIRONMENT geochronology gravel layer SEDIMENTOLOGY PLEISTOCENE Shandong Province
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Dynamics of the Coordination of Economy-Society and Resource-Environment in Shandong Peninsula,China 被引量:1
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作者 Han Rongqing Zhao Minghua 《Chinese Journal of Population,Resources and Environment》 2012年第3期62-71,共10页
Shandong Peninsula,as a more developed region in Shandong Province even the east coast of China,is facing challenges from resources and environment pressures.This paper tried to track and assess the coordination statu... Shandong Peninsula,as a more developed region in Shandong Province even the east coast of China,is facing challenges from resources and environment pressures.This paper tried to track and assess the coordination status and the dynamic between resource-environment and economy-society systems in Shandong Peninsula during 2001-2008 in order to provide decision support for regional sustainability.An appraisal index system was built including five aspects of harmony degree(A),sustainability degree(B),opening degree(C),stability degree(D) and controllability degree(E).The results showed that:1) The coordination level of resource-environment and economy-society in Shandong Peninsula has continuously grown,and it has undergone three stages:no coordinated degree(2001-2002),weak coordinated degree(2003-2006) and basically coordinated degree(2007-2008).2) Five indexes of criterion hierarchy also increased overall,but each index showed different trends.Harmony degree,sustainability degree and opening degree rose all the time,while stability degree and controllability degree alternately rose and fell.The improvement of controllability degree was the slowest.3) The aggravating trend of environmental pollution was slowing down.The economic growth was driven by industrial growth and urbanization typically and investment was still the main force to pull the regional economic growth.At the same time,technology and education were becoming more and more important for economic growth.The level of foreign capital utility declined and the geographical advantage of Shandong Peninsula was exerted.Meanwhile some characteristics of knowledge economy were presenting.Water resources become the main constraint factor of fast development in Shandong Peninsula.It is necessary to further strengthen the coordination ability of government on regional sustainable development. 展开更多
关键词 DYNAMICS coordination of resource-environment andeconomy-society Shandong Peninsula
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Estimation on wetland loss and its restoration potential in Modern Yellow River Delta,Shandong Province of China
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作者 Baolei Zhang Le Yin +1 位作者 Shumin Zhang Kai Liang 《Chinese Journal of Population,Resources and Environment》 2015年第4期365-372,共8页
Wetland is one of the most important ecosystems with varied functions and structures,and its loss has been a major issue.Wetland loss in Modem Yellow River Delta(MYRD) becomes a serious environmental problem,so its re... Wetland is one of the most important ecosystems with varied functions and structures,and its loss has been a major issue.Wetland loss in Modem Yellow River Delta(MYRD) becomes a serious environmental problem,so its restoration attracts a great deal of attention from academia and governments.This article proposes a GIS-based multi-criteria comprehensive evaluation methodology for potential estimation of wetland restoration,using MYRD as an example.The model uses four kinds of data(hydrology,terrain,soil,and land use) and could be adapted by planners for use in identifying the suitability of locations as wetland mitigation sites at any site or region.In the application of the model in the MYRD,the research developed a lost wetland distributed map taking the better wetland situation of 1995 as the reference,and elevated the overall distribution trends of wetland restoration potential based on wetland polygon.The results indicated that the total area of wetland loss from 1995 to 2014 was 568.12 km^2,which includes 188.83 km^2 natural wetland and 21.80 km^2 artificial wetland,respectively.The areas of lost wetland with low,middle,and high resilience ability are 126.82 km^2,259.92 km^2,and 119.59 km^2,occupying 25.05%,51.33%,and 23.62%,respectively.The high-restoration-potential wetland included98.47 km^2 of natural wetland and 21.12 km^2 of artificial wetland,which are mainly bush,reed,and ponds.The highrestoration-potential wetland is mainly distributed in the vicinity of Gudong oil field,the Yellow River Delta protected areas,and the eastern sides of Kenli county and Dongying city. 展开更多
关键词 WETLAND loss RESTORATION POTENTIAL comprehensive evaluation Modern YELLOW River DELTA
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Regional Study on Economical Supporting Capacity of Land Resources in Shandong Province
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作者 Li Yujiang Chen Pei'an 《Chinese Journal of Population,Resources and Environment》 北大核心 2007年第1期68-73,共6页
Taking the land resources of 17 cities in Shandong Province as the basic data, the article studied on the economical supporting capacity of land resources in terms of the effect of land on economy. The author classifi... Taking the land resources of 17 cities in Shandong Province as the basic data, the article studied on the economical supporting capacity of land resources in terms of the effect of land on economy. The author classified 17 cities of Shandong Province into four types according to the economical supporting capacity of land resources by quantitatively estimating the evaluation indices of the total amount of land resources, the potential of urban and other nonagricultural land, and the integrated economical sup- porting capacity of land resources, etc. The author proposes the questions requiring further study at the end of this article. 展开更多
关键词 land resources economical supporting capacity quantitative estimation Shandong Province
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Study on Dynamic Mechanism of Urban Expansion: A Case Study of Shandong Province
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作者 Zhang Xiaoqing Ren Jianlan 《Chinese Journal of Population,Resources and Environment》 北大核心 2007年第3期37-42,共6页
This paper aims at studying the dynamic mechanism of urban expansion and its role. Based on the economic model of urban spatial structure and applying panel data in Shandong Province, the paper concludes. Economic gro... This paper aims at studying the dynamic mechanism of urban expansion and its role. Based on the economic model of urban spatial structure and applying panel data in Shandong Province, the paper concludes. Economic growth and transition of economic structure have played the most powerful role in urban expansion. An increase in population helps the core to extend. Increasing agricultural productivity can provide a countervailing force. An increase in transportation costs will limit urban expan- sion. An increase in the marginal productivity of land in housing production will increase urban expansion. Fiscal and taxation policies also have encouraged the extensive shift of cultivated land into build-up area. This paper also suggests that if high rate increasing GDP is needed, the urban core will continue to expand. The government should take measures to improve the rapid growth of the city. 展开更多
关键词 urban extent dynamic mechanism economic modelof urban spatial structure panel data
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Analysis and Countermeasures on Effective Land Use of Rural Residential Area in Jinan, Shandong Province
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作者 Xu Hong 《Chinese Journal of Population,Resources and Environment》 北大核心 2006年第1期41-44,共4页
With the improvement of urbanization and the expansion of urban size, the cultivated land decreases gradually but the demand for urban land increases, thereby resulting in the conflict of land use. Analyzing the statu... With the improvement of urbanization and the expansion of urban size, the cultivated land decreases gradually but the demand for urban land increases, thereby resulting in the conflict of land use. Analyzing the status quo of land use of rural residential area, this paper points out that two main problems, namely, the low degree of land use, and the waste of land resources that are prevalent in the rural residential area. Based on the above analysis, the author states that the emphasis on land use will be the plain regions in the future, and adoption of reasonable economic measures will be the new solution to the problems. 展开更多
关键词 rural residential area effectively utilize movement and merger of villages
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Study on Development of the West of Shandong Province Based on Overall Regional Development Planning Pattern
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作者 Pang Jiewu 《Chinese Journal of Population,Resources and Environment》 2009年第4期82-87,共6页
The overall regional development planning pattern has been considered to be a new and effective pattern as for development of provincial economy.The article analyzed the current conditions of the west of Shandong Prov... The overall regional development planning pattern has been considered to be a new and effective pattern as for development of provincial economy.The article analyzed the current conditions of the west of Shandong Province and tried to find the generation mechanisms of current non-balanced economic development of Shandong Province.And then approaches to regional development were put forward according to the theory of regional development pattern as a whole. 展开更多
关键词 overall regional development planning pattern west of Shandong Province regional economy
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Taxonomic review on Acrocercopinae,Gracillariinae and Ornixolinae from Shandong,China,with new data on distribution and host associations(Lepidoptera:Gracillariidae)
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作者 Yurong Jiang Yang Zhao +2 位作者 Encui Wang Tongyou Zhang Tengteng Liu 《Zoological Systematics》 CSCD 2021年第3期240-257,共18页
Thirteen species of Gracillariidae,belonging to three subfamilies,Acrocercopinae,Gracillariinae and Ornixolinae,are reviewed from Shandong,China.Three species are recorded from China for the first time:Calybites secur... Thirteen species of Gracillariidae,belonging to three subfamilies,Acrocercopinae,Gracillariinae and Ornixolinae,are reviewed from Shandong,China.Three species are recorded from China for the first time:Calybites securinella(Ermolaev,1986),Eteoryctis picrasmae Kumata&Kuroko,1988,and Spulerina parthenocissi Kumata&Kuroko,1988.All species are described in detail except Liocrobyla indigofera Liu,Wang&Wang,2018,with the illustrations of adults,male and female genitalia. 展开更多
关键词 Host plants new record China MICROLEPIDOPTERA
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Improving the Service Level of University Library by Using Micro-Blog and WeChat
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作者 Qin Zhang 《Journal of Educational Theory and Management》 2017年第1期135-136,共2页
In order to improve the service level of the University Library and increase the utilization rate of the students' libraries, the domestic libraries began to use micro-blog and WeChat. The use of micro-blog and We... In order to improve the service level of the University Library and increase the utilization rate of the students' libraries, the domestic libraries began to use micro-blog and WeChat. The use of micro-blog and WeChat is in line with the characteristics of college students and can improve the service level of University libraries effectively. In this article, I will start with introducing micro- blog and WeChat, briefly describe the current situation of using micro-blog and WeChat in university libraries, and the measures to improve the service level of university libraries by using micro-blog and WeChat. 展开更多
关键词 WeChat Micro-Blog UNIVERSITY LIBRARY SERVICE LEVEL
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Research on the model of regional green innovation system in China:A case study of Shandong province
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作者 LI Hong-fei CI Fu-yi SHI Jia-lu 《Ecological Economy》 2020年第2期146-160,共15页
Taking Shandong province,one of China’s first batch of circular economy pilot provinces,as an example,this paper summarizes some types of regional green innovation system models through the cases study and retrospect... Taking Shandong province,one of China’s first batch of circular economy pilot provinces,as an example,this paper summarizes some types of regional green innovation system models through the cases study and retrospective reasoning and retrospective approaches.The models mentioned above include(1)the innovation system model of green animal husbandry in coastal small towns,(2)the green innovation system model in intravenous industry park,(3)the green innovation system model in coastal hi-tech industrial development zone based on the development of eco-industrial and low-carbon industries,(4)the green innovation system model in coastal economic&technological development area based on the development of ecological arterial industry and the ecological intravenous industry through the waste recycle inside and outside the area,(5)the green innovation system model of coastal harbor economic and technological development area based on constructing tiny-medium-macro circulation,(6)the green innovation system of reformed coastal economic and technological development zone turning to the recycling marine industry innovation,and(7)the green innovation system model of high-tech industry development zone tending to recycling innovation of biological industry.This paper aims to analyze the characteristics of these models,the formation mechanism,and further to discuss their implications for greening the same type of regional innovation systems. 展开更多
关键词 green innovation system low-carbon development circular economy MODEL Shandong provinces
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Backstepping-Based Distributed Abnormality Detection for Nolinear Parabolic Distributed Prameter Systems
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作者 Lei Chen 《Engineering(科研)》 CAS 2022年第7期285-299,共15页
In this paper, we proposed a model-based abnormality detection scheme for a class of nonlinear parabolic distributed parameter systems (DPSs). The proposed methodology consists of the design of an observer and an abno... In this paper, we proposed a model-based abnormality detection scheme for a class of nonlinear parabolic distributed parameter systems (DPSs). The proposed methodology consists of the design of an observer and an abnormality detection filter (ADF) based on the backstepping technique and a limited number of in-domain measurements plus one boundary measurement. By taking the difference between the measured and estimated outputs from observer, a residual signal is generated for fault detection. For the detection purpose, the residual is evaluated in a lumped manner and we propose an explicit expression for the time-varying threshold. The convergence properties of the PDE observer and the residual are analyzed by Lyapunov stability theory. Eventually, the proposed abnormality detection scheme is demonstrated on a nonlinear DPS. 展开更多
关键词 Abnormality Detection BACKSTEPPING Nonlinear Parabolic Systems Distributed Parameter Systems Lyapunov Function
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Investigation and Analysis of Crop Germplasm Resources in Coastal Areas of Shandong Province
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作者 Dong WANG Xiaodong ZHANG +11 位作者 Runfang LI Zhan LI Yumin MA Yu ZHANG Nana LI Weijing CHEN Zhongxue FAN Shoujin FAN Libin ZHANG Hui ZHANG Yingjie LIN Hanfeng DING 《Agricultural Science & Technology》 CAS 2017年第10期1825-1831,共7页
This study focused on the investigation of crop germplasm resources in coastal areas of Shandong Province, including 132 villages in 82 towns of 34 counties. The survey collected local varieties and wild resources of ... This study focused on the investigation of crop germplasm resources in coastal areas of Shandong Province, including 132 villages in 82 towns of 34 counties. The survey collected local varieties and wild resources of grain crops, economic crops, vegetables and fruit trees, and a total of 848 samples were collected, belonging to 54 species of 39 genera in 15 families. In this study, the current situation and growth and decline conditions of crop germplasm resources were investigated, and their botanical classification and utilization importance were analyzed. Furthermore, the conservation, development and utilization of crop germplasrp resources in coastal areas of Shandong Province were also discussed in this paper. 展开更多
关键词 Coastal areas CROP Germplasm resource Local varieties INVESTIGATION
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Identification,Isolation and Genomic Characterization of Porcine Astrovirus in Shandong Province,China in 2021-2023
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作者 Liu Yueyuel Zhang Yu +7 位作者 Zhu Yuwei Guo Xiaozhen Yang Shifa Yin Bin Zhao Zengcheng Huang Zhonglil Wu Jiaqiang Lin Shuqian 《Animal Husbandry and Feed Science》 CAS 2024年第1期13-20,共8页
[Objective]The paper was to identify,isolate,and characterize porcine astrovirus in Shandong Province between 2021 and 2023.[Method]A total of 1025 samples of porcine diarrhea samples were collected from various regio... [Objective]The paper was to identify,isolate,and characterize porcine astrovirus in Shandong Province between 2021 and 2023.[Method]A total of 1025 samples of porcine diarrhea samples were collected from various regions of Shandong Province between January 2021 and October 2023.The samples were tested by RT-PCR,followed by sequencing and phylogenetic analyses of the polymerase.[Result]The total positive rate of PAstV was 34.6%(355/1025).The respective proportions of individuals infected with PAstV-1,PAstV-2,PAstV-4 and PAstV-5 were 25.4%(90/355),28.2%(100/355),35.2%(125/355)and 22.5%(80/355),respectively.Additionally,mixed infection was observed.Meanwhile,849 samples of healthy pigs were tested by RT-PCR,and the results demonstrated that the total positive rate of PAstV was 8.13%(69/849).Of these,the proportion of PAstV-1,PAstV-2 and PAstV-4 infection was 27.5%(19/69),37.7%(26/69)and 40.6%(28/69),and a mixed infection also existed.Further sequencing and characterization of some the selected isolates revealed low sequence identities(56.2%)with known PAstV strains,indicating the presence of novel types or genotypes of PAstVs.Furthermore,the isolation conditions of porcine astrovirus were optimized,resulting in the purification of a pure PAstV-4 strain(designated PAstV-4-GRF1).The virus was found to exhibit typical astroviral morphology,with nucleotide identity ranging from 89.9 to 95.4%with previously published PAstV-4 strains.Then,macrovirus transcriptome sequencing showed that 88.30%of the CRF1 samples were mammalian astroviruses.By species classification,PAstV 4 and PAstV 2 accounted for 21.79%and 0.32%,respectively.Phylogenetic tree analysis showed that the c15050 fragment was identical to the GRF-1 sequencing fragment of the isolated strain,and exhibited the highest homology with the Hunan PAstV-4 sequence MK460231 in China.[Conclusion]As the inaugural isolated PAstV-4 strain,it furnishes pivotal materialfor the investigation ofthe biological and pathogenic properties of this virus as well as for the prospectivedevelopment of relevant biological and diagnostic reagents. 展开更多
关键词 Porcine astrovirus Epidemic situation Genotype analysis Isolation and identification
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