Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community asse...Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community assembly in virgin and aging MP plastispheres across different habitats is poorly understood.This study aims to assess the variations in bacterial community assembly across different niches and habitats with an in situ ex-periment,in which constructed forest wetland(FW),natural lake wetland(LW),and lotus pond wetland(LP)were habitats,and plastispheres of virgin and aging low-density polyethylene(LDPE)MPs,as well as surround-ing wetland soils were niches.Significant niche-related differences in bacterial communities were observed,with lower diversity and enrichment of potential plastic-degrading bacteria in the plastisphere than in the soil bacterial communities.Furthermore,habitat-related differences exerted a more pronounced influence on the beta-diversity patterns of the bacterial communities.The linear regression analyses indicated that the local species pool con-tributed more to bacterial community assembly in the LW wetland,whereas the relative abundance of species was the primary factor in the LP wetland.The null model analysis indicated that plastisphere bacterial communi-ties were predominantly driven by the stochastic process,with a more deterministic assembly observed in the LP wetland and soil bacterial communities.Additionally,the primary ecological process shaping plastisphere com-munities shifted from drift in the virgin LDPE to homogenising dispersal in the aging LDPE.This study provides new insights into the fate and ecological impacts of MPs in wetlands,thereby facilitating the effective regulations of plastic pollution.展开更多
During the initial impoundment period of a canyon-shaped reservoir,the water body fluctuated violently regarding water level,hydrological condition,and thermal stratification.These variations may alter the structure o...During the initial impoundment period of a canyon-shaped reservoir,the water body fluctuated violently regarding water level,hydrological condition,and thermal stratification.These variations may alter the structure of phytoplankton community,resulting in algal blooms and seriously threatening the ecological security of the reservoir.It is of great significance to understand the continuous changes of phytoplankton in the initial impoundment period for the protection of reservoir water quality.Therefore,a two-year in-situ monitoring study was conducted on water quality and phytoplankton in a representative canyonshaped reservoir named Sanhekou and the interannual changes of phytoplankton community and its response to environmental changes during the initial impoundment period were discussed at taxonomic versus functional classification levels.The results showed that the total nitrogen and permanganate index levels were relatively high in the first year due to rapid water storage and heavy rainfall input,and the more stable hydrological conditions in the second year promoted the increase of algae density and the transformation of community,and the proportion of cyanobacteria increased significantly.The succession order of phytoplankton in the first year of the initial impoundment periodwas Chlorophyta-Bacillariophyta-Chlorophyta,or J/F/X1-P/MP/W1-A/X1/MP,respectively.And the succession order in the second year was Cyanobacteria/Chlorophyta-Bacillariophyta-Chlorophyta,or L_(M)/G/P-P/A/X1-X1/J/G.Water temperature,relativewater column stability,mixing depth,and pHwere crucial factors affecting phytoplankton community succession.This study revealed the interannual succession law and driving factors of phytoplankton in the initial impoundment period and provided an important reference for the operation management and ecological protection of canyon-shaped reservoirs.展开更多
Graphene oxide nanomaterials are increasingly used in various fields due to their superior properties.In order to study the influence of graphene oxide additives on the performance of modified asphalt,in this study,gr...Graphene oxide nanomaterials are increasingly used in various fields due to their superior properties.In order to study the influence of graphene oxide additives on the performance of modified asphalt,in this study,graphene oxide modified asphalt was prepared and characteristics was studied including the high deformation resistance performance and the self-healing property of modified asphalt.Functional groups and morphology of graphene oxide modified asphalt were described by Fourier transform infrared spectroscopy.The high deformation resistance performance and self-healing effect of asphalt samples were obtained through dynamic slear rheometer(DSR)test.Results shows that graphene oxide dispersions improve the performance of asphalt relatively well compared to graphene oxide powder.There is no chemical reaction between graphene oxide and asphalt,but physical connection.The addition of graphene oxide improved the high deformation resistance of modified asphalt and expedited the self-healing ability of asphalt under fatigue load.展开更多
This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each...This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each set of trials contained a control(de-chlorinated tap water),and the experiments were repeated three times.The fish(n=10)were randomly measured with an average length of(3.4±0.34)cm and weight of(1±0.1)g.The temperature was kept at 24℃.Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish(Danio rerio)respectively,whereas experiment 3 was aimed at the combined toxicity of IMI and THM on zebrafish.The tests followed the same study design,and each experiment used seven different logarithmic concentrations of imidacloprid insecticides(310.00,317.08,324.33,331.74,339.32,347.07,355.00 mg/L)and thiamethoxam(175.00,185.52,200.93,215.30,230.70,247.20,264.88 mg/L).The results show that THM is more toxic than IMI,with LC_(50)values of 190.34 mg/L for THM and 310.92 mg/L for IMI.Both individual toxicities showed a substantial positive connection(P<0.05)with confidence limits of 321.50-300.68 mg/L for IMI and 199.91-181.21 mg/L for THM.The joint toxicity test was carried out using the 96-h LC_(50)values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio.The Additive Index(AI)demonstrated that imidacloprid and thiamethoxam acted synergistically on D.rerio.As a matter of fact,more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life.展开更多
In this study,the single and jiont acute toxicity effects of pendimethalin(herbicide)and fenitrothion(organophosphate insecticide)were investigated on juvenile zebrafish(Danio rerio)under semi-static conditions.Mortal...In this study,the single and jiont acute toxicity effects of pendimethalin(herbicide)and fenitrothion(organophosphate insecticide)were investigated on juvenile zebrafish(Danio rerio)under semi-static conditions.Mortality was assessed at 24,48,72,and 96 h.The study revealed that pendimethalin exhibited higher toxicity than fenitrothion.The 96-h LC 50 values were 0.477 mg/L for pendimethalin and 2.634 mg/L for fenitrothion.Joint exposure produced enhanced toxicity,with 96-h LC 50 values of 0.204 mg/L(pendimethalin equivalent)and 1.139 mg/L(fenitrothion equivalent).Regression analysis showed a significant positive correlation(p<0.05)between pesticide concentration and mortality,while toxicity indices confirmed synergistic interactions.These findings underscore the ecological risks posed by pesticide mixtures and highlight the importance of regulating pesticide use to safeguard aquatic organisms and maintain environmental sustainability.展开更多
Flood regulation service(FRS)stands as one of the key benefits that people get from the ecosystem.Under the influence of climate change and human activities,the relationship between supply and demand of FRS would incr...Flood regulation service(FRS)stands as one of the key benefits that people get from the ecosystem.Under the influence of climate change and human activities,the relationship between supply and demand of FRS would increasingly affect regional flood risk and sustainable development.However,there was currently a lack of systematic study on the future supply-demand relationship of FRS in the flood-vulnerable area undergoing rapidly development in China.This study integrated the Scenario Model Intercomparison Project(ScenarioMIP)with the Shared Socioeconomic Pathways(SSPs)datasets and climate model data to quantify the supply-demand ratio(SDR)of FRS in the Yangtze River Delta(YRD),China from 2020 to 2050.Trend analyses were conducted using linear regres-sion,Theil-Sen median estimation,and Hurst exponent analysis,while key drivers of SDR changes were identified and quantified through the Lindeman-Merenda-Gold(LMG)method between 2021 and 2050.Results show that the supply of FRS in the YRD was generally insufficient to meet the demand.The imbalanced subbasins covered 88.24%of the total study area,with 34.48%of this imbal-anced area concentrated in the Southeastern Basin in China.During 2021 and 2050,the imbalance of FRS supply-demand relationship would largely aggravate in the YRD,of which the aggravated area would account for 77.23%.Under different scenarios,the SDR for FRS would decrease significantly,with rates ranging from-5.45×10^(-4) to-2.06×10^(-4)(P<0.05).Especially,the decline rate of SDR in the YRD Basin(DeltaB)reached 2.92 times that the average of YRD.Human activities were the primary factors that exacerbated the imbalance in FRS supply-demand relationship,of which the relative contribution rate exceeds 75%.Particular attention should be direc-ted toward critical regions like the Southeast Basin in China(SEB)and DeltaB where substantial aggravation of supply-demand imbal-ances of FRS is projected.展开更多
In-situ enhanced bioreduction by functional materials is a cost-effective technology to remove chlorinated hydrocarbons in groundwater.Herein,a novel polydopamine(PDA)-modified biochar(BC)-based composite containing n...In-situ enhanced bioreduction by functional materials is a cost-effective technology to remove chlorinated hydrocarbons in groundwater.Herein,a novel polydopamine(PDA)-modified biochar(BC)-based composite containing nanoscale zero-valent iron(n ZVI)and poly-l-lactic acid(PLLA)(PB-PDA-Fe)was synthesized to enhance the removal of 1,1,1-trichloroethane(1,1,1-TCA)in simulated groundwater with actual site sediments.Its impact on functional microbial community structure in system was also investigated.The typical characterizations revealed uniform dispersion of PLA and n ZVI particles on the BC surface,being smoother after PDA coating.The composite exhibited a significantly higher performance on 1,1,1-TCA removal(82.38%,initial concentration 100 mg/L)than Fe-PDA and PB-PDA treatments.The diversity and richness of the microbial community in the composite treatment consistently decreased during incubation due to a synergistic effect between PLLA-BC and n ZVI.Desulfitobaterium,Pedobacter,Sphaerochaeta,Shewanella,and Clostridium were identified as enriched genera by the composite through DNA-stable isotope probing(DNA-SIP),playing a crucial role in the bioreductive dechlorination process.All the above results demonstrate that this novel composite selectively enhances the activity of microorganisms with extracellular respiration functions to efficiently dechlorinate 1,1,1-TCA.These findings could contribute to understanding the responsive microbial community by carbon-iron composites and expedite the application of in-situ enhanced bioreduction for effective remediation of chlorinated hydrocarbon-contaminated groundwater.展开更多
Exploring the suitability of biochar for improving soil quality under different water and salt conditions is important for maintaining soil health and productivity in the arid regions of northwestern China.We compared...Exploring the suitability of biochar for improving soil quality under different water and salt conditions is important for maintaining soil health and productivity in the arid regions of northwestern China.We compared the effects of biochar application practices on soil physical,chemical and biological properties under different irrigation and water salinity levels in a two-year field experiment in a mulched and drip-irrigated maize field in Gansu Province,China.Eight treatments in total included the combination of two biochar addition rates of 0 t ha–1(B0)and 60 t ha–1(B1),two irrigation levels of full(W1)and deficit irrigation(W2;W2=1/2 W1)and two water salinity levels of fresh water(S0,0.71 g L–1)and brackish water(S1,4.00 g L–1).The minimum dataset method was used to calculate the soil quality index(SQI)under different treatments.Deficit and brackish water irrigation significantly reduced SQI by 3.80–9.80%through reducing some soil physical,chemical and biological properties.Biochar application significantly increased the SQI by 6.13 and 10.40%under full irrigation with fresh and brackish water,respectively.Biochar addition enhanced the relative abundance of beneficial bacteria(e.g.,Proteobacteria,Patescibacteria)in the soil in all water–salt treatments.The partial least squares path model showed that biochar application significantly enhanced the SQI mainly by improving soil aggregation and pore structure under particular water–salt conditions.This research provides an important basis for utilizing biochar to improve soil quality in arid regions of Northwest China under various water–salt conditions.展开更多
The extensive co-occurrence of pyrethroid insecticides such as lambda-cyhalothrin(LCT)and cypermethrin(CPM)in aquatic systems poses a potential risk,yet a significant research gap exists regarding their combined toxic...The extensive co-occurrence of pyrethroid insecticides such as lambda-cyhalothrin(LCT)and cypermethrin(CPM)in aquatic systems poses a potential risk,yet a significant research gap exists regarding their combined toxicological effects.In this study,the single and joint acute toxicity effects of lambda-cyhalothrin(LCT)and cypermethrin(CPM)on Nile tilapia fingerlings were investigated using 96-h bioassays.Results showed both were highly toxic,with LCT(96-h LC 50=66.53μg/L)being four-fold more potent than CPM(259.41μg/L).Regression analysis confirmed positive correlation(P<0.01)between pesticide concentration and observed mortality.The binary mixture exhibited synergistic effect with Additive Index(AI)>0,indicating combined effects exceeded the sum of their individual actions.This synergism likely stems from mutual inhibition of metabolic detoxification pathways,leading to increased internal concentrations and amplified neurotoxicity.Generally,this study confirmed that single-compound risk assessments dangerously underestimate pyrethroid mixture hazards,necessitating their inclusion in regulatory frameworks for accurate aquatic biodiversity protection.展开更多
Carbon storage serves as a key indicator of ecosystem services and plays a vital role in maintaining the global carbon balance.Land use and cover change(LUCC)is one of the primary drivers influencing carbon storage va...Carbon storage serves as a key indicator of ecosystem services and plays a vital role in maintaining the global carbon balance.Land use and cover change(LUCC)is one of the primary drivers influencing carbon storage variations in terrestrial ecosystems.Therefore,evaluating the impacts of LUCC on carbon storage is crucial for achieving strategic goals such as the China’s dual carbon goals(including carbon peaking and carbon neutrality).This study focuses on the Aral Irrigation Area in Xinjiang Uygur Autonomous Region,China,to assess the impacts of LUCC on regional carbon storage and their spatiotemporal dynamics.A comprehensive LUCC database from 2000 to 2020 was developed using Landsat satellite imagery and the random forest classification algorithm.The integrated valuation of ecosystem services and trade-offs(InVEST)model was applied to quantify carbon storage and analyze its response to LUCC.Additionally,future LUCC patterns for 2030 were projected under multiple development scenarios using the patch-generating land use simulation(PLUS)model.These future LUCC scenarios were integrated with the InVEST model to simulate carbon storage trends under different land management pathways.Between 2000 and 2020,the dominant land use types in the study area were cropland(area proportion of 35.52%),unused land(34.80%),and orchard land(12.19%).The conversion of unused land and orchard land significantly expanded the area of cropland,which increased by 115,742.55 hm^(2).During this period,total carbon storage and carbon density increased by 7.87×10^(6) Mg C and 20.19 Mg C/hm^(2),respectively.The primary driver of this increase was the conversion of unused land into cropland,accounting for 49.28%of the total carbon storage gain.Carbon storage was notably lower along the northeastern and southeastern edges.By 2030,the projected carbon storage is expected to increase by 0.99×10^(6),1.55×10^(6),and 1.71×10^(6) Mg C under the natural development,cropland protection,and ecological conservation scenarios,respectively.In contrast,under the urban development scenario,carbon storage is projected to decline by 0.40×10^(6) Mg C.In line with China’s dual carbon goals,the ecological conservation scenario emerges as the most effective strategy for enhancing carbon storage.Accordingly,strict enforcement of the cropland red line is recommended.This study provides a valuable scientific foundation for regional ecosystem restoration and sustainable development in arid regions.展开更多
Human-wildlife conflict(HWC)and its socioeconomic impacts are a pressing global issue.Accurately quantifying HWCs and their interaction with residential development is crucial for rural revitalization and biodiversity...Human-wildlife conflict(HWC)and its socioeconomic impacts are a pressing global issue.Accurately quantifying HWCs and their interaction with residential development is crucial for rural revitalization and biodiversity conservation efforts.This study investigates the interplay between rural residential expansion(RRE)with humanelephant conflict(HEC)in southern Yunnan Province using high-accuracy yearly land use/land cover data and Asian elephant accident data.A piecewise regression along with several metrics,including expansion intensity,rate of rural residential land,and residential density,were employed to analyze the spatial-temporal change characteristics of RRE.Then,a geographical detector and a bivariate spatial autocorrelation model were used to reveal the driving mechanisms of RRE,with particular emphasis on the spatial relations between RRE and HECs.The results indicate that HECs had a significant negative impact on RRE,exhibiting higher expansion intensity and rate of rural residential land in non-HEC areas than in HEC areas.High spatiotemporal consistency between accelerated RRE and intensified HECs occurred from 2010 onwards,which aligns with the year when the trend of settlement area expansion changed.RRE activities and ensuing land use conversions led to increased occurrences of HECs,which negatively affected the RRE.Compared to HECs,topography and locational factors exhibited a secondary effect on RRE activities.The findings underscore reciprocal feedback mechanisms between RRE and HECs and the elevated risk of adverse interactions between humans and elephants within the range of China’s wild elephants,providing theoretical support for coordinating conservation initiatives for Asian elephants with rural revitalization in the border areas of Southwest China.展开更多
Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we gen...Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.展开更多
The red tilefish Branchiostegus japonicus is an economically important demersal fish species with high-quality.However,research on its reproductive biology and population structure characteristics is limited.To better...The red tilefish Branchiostegus japonicus is an economically important demersal fish species with high-quality.However,research on its reproductive biology and population structure characteristics is limited.To better understand the reproductive biological features of B.japonicus,a study was conducted on 461 individuals collected from the East China Sea(27°00′-31°00′N,122°30′-127°30′E)from March to December in 2021.This study analyzed the relationship between standard length and the body weight,and detected the sex ratio,length at first sexual maturity,reproductive period,fecundity,and spawning type of this species.The results revealed a power function relationship between body weight(W)and standard length(L)in the samples as the following regression equation:W=0.000014 L^(3.16)(R^(2)=0.956).The sex ratio was close to 1:1(χ^(2)=1.11,P>0.05),and the sex ratio exhibited significant differences according to different standard lengths.Using the logistic equation,we estimated that 50% of B.japonicus individuals had a standard length at sexual maturity of L_(50)=210 mm.By analyzing the monthly variation in the gonadal index and the proportion of each stage of ovarian development in each month,we assumed that the spawning period of B.japonicus occurred from May to November,and the peak spawning period occurred from June to October.The absolute fecundity was 51441±33232 eggs,the relative fecundity at the standard length was(210±117)eggs mm-1,and the relative fecundity relative to body weight was(184±89)eggs g^(-1).The distribution of the egg diameter of B.japonicus had only one obvious peak,indicating the spawning pattern of B.japonicus was once a year.This study updated and enriched the basic biological data of B.japonicus,which is beneficial for obtaining a better understanding of its population dynamics,and promoting the conservation and sustainable utilization of B.japonicus in East China Sea.展开更多
The sugar beet cyst nematode(Heterodera schachtii) is one of the most destructive pathogens in sugar beet production, which causes serious economic losses every year. Few molecular details of effectors of H. schachtii...The sugar beet cyst nematode(Heterodera schachtii) is one of the most destructive pathogens in sugar beet production, which causes serious economic losses every year. Few molecular details of effectors of H. schachtii parasitism are known. We analyzed the genome and transcriptome data of H. schachtii and identified multiple potential predicted proteins. After filtering out predicted proteins with high homology to other plant-parasitic nematodes, we performed functional validation of the remaining effector proteins. 37 putative effectors of H. schachtii were screened based on the Nicotiana benthamiana system for identifying the effectors that inhibit plant immune response, eventually determines 13 candidate effectors could inhibit cell death caused by Bax. Among the 13 effectors, nine have the ability to inhibit GPA2/RBP1-induced cell death. All 13 effectortriggered immunity(ETI) suppressor genes were analyzed by qRT-PCR and confirmed to result in a significant downregulation of one or more defense genes during infection compared to empty vector. For in situ hybridization,13 effectors were specifically expressed and located in esophageal gland cells. These data and functional analysis set the stage for further studies on the interaction of H. schachtii with host and H. schachtii parasitic control.展开更多
Grain soybean(Glycine max(L.)Merr.)shows potential as a forage crop following its introduction from low to high latitudes due to in its growth phenotype.However,the application of this approach is impeded by the diver...Grain soybean(Glycine max(L.)Merr.)shows potential as a forage crop following its introduction from low to high latitudes due to in its growth phenotype.However,the application of this approach is impeded by the diverse ecological types of soybean,their adaptability to the introduction,and the uncertainty surrounding the growth phenotype post-introduction.This study introduced 24 grain soybean varieties from low-latitude regions(22-31°N)to the high-latitude northwestern Loess Plateau(39°N)between 2018 and 2019.The growth phenotypes were observed,and their forage productivity potential was assessed.All varieties displayed delayed flowering following cross-latitude introduction,with the vegetative growth phase of some varieties even exceeding their entire growth duration at their origin.Rapid dry matter accumulation rates indicated growth adaptation performance and formed the basis for soybean forage yield.Varieties from the tropical South China region exhibited significant yield advantages,with dry matter yields of 8.97-14.68 t ha^(-1)and crude protein yields of 1.44-2.51 t ha^(-1).Varieties HX3 and GX7 from this region demonstrated optimal growth adaptability and productivity in the cross-latitude environment,achieving the highest dry matter yields of 14.68 and 13.86 t ha^(-1),respectively.As a result,HX3 and GX7 are recommended for local farming systems to provide high-quality forage.The cross-latitude introduction of soybean is proposed as a viable and efficient strategy for forage improvement and application.展开更多
Based on the observational data from 60 short-period stations deployed in the Jishishan M6.2 earthquake epicenter and adjacent regions(Gansu Province,2023),this study inverted the near-surface S-wave velocity structur...Based on the observational data from 60 short-period stations deployed in the Jishishan M6.2 earthquake epicenter and adjacent regions(Gansu Province,2023),this study inverted the near-surface S-wave velocity structure through teleseismic receiver function analysis by using the amplitude of direct P-wave.The results reveal that the epicentral area(Liugou Township and surroundings)exhibits markedly low S-wave velocities of 400-600 m/s,with a mean value of(500±50)m/s.In contrast,intermountain basins-Guanting Basin and Dahejia Basin-demonstrate significantly elevated velocities,exceeding the epicentral zone by 100-300 m/s,with values concentrated at 600-900 m/s.Notably,localized areas such as Jintian Village and Caotan Village maintain stable S-wave velocities of(700±30)m/s.The western margin tectonic belt of Jishishan displays distinctive velocity differentiation:A pronounced velocity gradient zone along the 35.8°N latitude boundary separates northern areas(<550 m/s)from southern regions(>750 m/s).These findings demonstrate significant spatial heterogeneity in shallow S-wave velocity structures,primarily controlled by three factors:(1)topographic-geomorphic units,(2)stratigraphic lithological contrasts,and(3)anthropogenic modifications.The persistent low-velocity anomalies(<600 m/s)in the epicentral zone and northern Yellow River T2 terrace likely correlate with Quaternary unconsolidated sediments,enhanced groundwater circulation,and bedrock weathering.These results provide critical geophysical constraints for understanding both the seismogenic environment of the Jishishan earthquake and its damage distribution patterns.Furthermore,they establish a foundational framework for regional seismic intensity evaluation,site amplification analysis,and secondary hazard risk assessment.展开更多
Soil denitrification,anammox,and Feammox are key for nitrogen(N)removal in agriculture.Despite potassium(K)fertilizer enhancing N efficiency,their role in regulation of these processes is unclear.A soil column incubat...Soil denitrification,anammox,and Feammox are key for nitrogen(N)removal in agriculture.Despite potassium(K)fertilizer enhancing N efficiency,their role in regulation of these processes is unclear.A soil column incubation with 15N isotope tracingwas conducted to explore millimeter-scale interactions of N and K on these pathways in soil fertilization zones.After 28 days,individual applications of N and K reduced denitrification-nitrogen removal rate(DNRR),anammox-nitrogen removal rate(ANRR),and feammox-nitrogen removal rate(FNRR)compared to a non-fertilizer control.N fertilizer had a greater effect than K,likely due to the high consumption of dissolved organic carbon by N fertilizer or the increased soil organic matter decomposition by K fertilizer.Combing of N and K increased DNRR,ANRR and FNRR rates by 31%,3090%and 244%compared to single N,and by-53.7%,885%and 222%compared to single K.These effects diminished with depth and distance from fertilizer sites.The effects of N fertilizer on these N removal processes might be regulate abundance of key microbes(e.g.,Limnobacter and Clostridium)and key gene(nirK,hzsB,ACM and Geo)by providing N substrates,while K enhances N metabolism efficiency through enzyme activation,indicated by the downregulation of certain genes(hzsB,ACM and Geo)and a negative correlation with N removal by simultaneously increasing gene expression and enzyme activity.These findings provide insights into how N and K together enhance N removal,emphasizing their importance for optimizing this process.展开更多
Surge-type glaciers are widely developed in mountainous areas around the world.Understanding the trigger mechanism of glacier surge is a prerequisite for addressing their impacts on hydrological assessments,disentangl...Surge-type glaciers are widely developed in mountainous areas around the world.Understanding the trigger mechanism of glacier surge is a prerequisite for addressing their impacts on hydrological assessments,disentangling climate-glacier linkages,and mitigating downstream hazards.Most glacier surges occur in the compound glaciers;however,attention paid to the trigger mechanisms of such surges is minimal.This study confirmed two surges in the northern and southern branches of the Aru-4 glacier,respectively,in the Western Tibetan Plateau,using multisource remote sensing data.The northern branch of the Aru-4 glacier entered the active phase in 1999 and the active phase lasted for 6 years.The southern branch of the Aru-4 glacier entered the active phase in 2007 and the active phase lasted for 9 years.The southern branch of the Aru-4 glacier experienced a long period of retreat before the northern branch surged and their tongues were in a detached state.The northern branch surge carried a large amount of ice to the frontal area,blocking the downward transport of ice from the southern branch and initiated surge.Through the analysis of two surge processes of Aru-4 glacier,we found a new surge mechanism for compound glaciers.It was revealed that surges in such glaciers are not only triggered by the reduction in basal sliding resistance caused by the internal factors.These surges initiated in the upper part of the glacier then propagated to down glacier by intense compression force.Furthermore,surges can also be triggered by external intervention of blocking by other branches.This external trigger initiates the surge in the lower part of the glacier then propagated to the upper part by longitudinal traction force.In addition,comparing with the surge triggered by the internal factors,the surge triggered by the external intervention may have a more dramatic process.展开更多
This study investigates the spatial distribution and regulatory mechanisms of dissolved inorganic carbon(DIC) in Xiangshan Bay, East China Sea, addressing critical gaps in carbon cycling research within semi-enclosed ...This study investigates the spatial distribution and regulatory mechanisms of dissolved inorganic carbon(DIC) in Xiangshan Bay, East China Sea, addressing critical gaps in carbon cycling research within semi-enclosed bays. Through isotopic analysis(δ^(13)C_(DIC), δD and δ^(18)O) and hydrochemical measurements [salinity and dissolved oxygen(DO)] of surface and bottom seawater samples from 52 stations, we demonstrate that δ^(13)C_(DIC) values(surface:-3.6‰ to-2.1‰;bottom:-3.6‰ to-1.8‰) exhibit distinct vertical and spatial patterns, with higher values in surface waters and outer bay regions compared to bottom and inner bay areas. Conservative mixing between seawater(average contribution:56%) and freshwater dominates DIC dynamics, while tidal hydrodynamics amplify the imprint of riverine inputs during low tides. Nutrient gradients driven by saline-freshwater mixing enhance primary productivity in outer bay regions,resulting in 13C-enriched DIC and elevated dissolved organic carbon(DOC) concentrations. Conversely, bottom waters show 13C-depleted signatures(-2.75‰ mean δ^(13)C_(DIC)), reflecting organic matter degradation under oxygen-depleted conditions. Aquaculture activities exacerbate localized eutrophication, with monsoon-enhanced runoff amplifying anthropogenic impacts. This work underscores the sensitivity of coastal carbon cycling to both natural hydrodynamics and anthropogenic perturbations in semi-enclosed bays.展开更多
In recent years,intensive human activities have increased the intensity of desertification,driving continual desertification process of peripheral meadows.To investigate the effects of restoration on soil microbial co...In recent years,intensive human activities have increased the intensity of desertification,driving continual desertification process of peripheral meadows.To investigate the effects of restoration on soil microbial communities,we analyzed vegetation-soil relationships in the Hulun Buir Sandy Land,northern China.Through the use of high-throughput sequencing,we examined the structure and diversity in the bacterial and fungal communities within the 0-20 cm soil layer after 9-15 a of restoration.Different slope positions were analyzed and spatial heterogeneity was assessed.The results showed progressive improvements in soil properties and vegetation with the increase of restoration duration,and the following order was as follows:bottom slope>middle slope>crest slope.During the restoration in the Hulun Buir Sandy Land,the bacterial communities were dominated by Proteobacteria,Actinobacteria,and Acidobacteria,whereas the fungal communities were dominated by Ascomycota and Basidiomycota.Eutrophic bacterial abundance increased with the restoration duration,whereas oligotrophic bacterial and fungal abundance levels decreased.The soil bacterial abundance significantly increased with the increasing restoration duration,whereas the fungal diversity decreased after 11 a of restoration,except that at the crest slope.Redundancy analysis showed that pH,soil moisture content,total nitrogen,and vegetation-related factors affected the bacterial community structure(45.43%of the total variance explained).Canonical correspondence analysis indicated that pH,total phosphorus,and vegetation-related factors shaped the bacterial community structure(31.82%of the total variance explained).Structural equation modeling highlighted greater bacterial responses(R^(2)=0.49-0.79)to changes in environmental factors than those of fungi(R^(2)=0.20-0.48).The soil bacterial community was driven mainly by pH,soil moisture content,electrical conductivity,plant coverage,and litter dry weight.The abundance and diversity of the soil fungal community were mainly driven by plant coverage,litter dry weight,and herbaceous aboveground biomass,while there was no significant correlation between the soil fungal community structure and environmental factors.These findings highlighted divergent microbial succession patterns and environmental sensitivities during sandy grassland restoration.展开更多
基金supported by Shanghai Municipal Natural Science Foundation,China(No.21ZR1446800)the National Natural Science Foundation of China(No.41877425)the Fundamental Research Funds for the Central Universities(No.226-2024-00052)。
文摘Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community assembly in virgin and aging MP plastispheres across different habitats is poorly understood.This study aims to assess the variations in bacterial community assembly across different niches and habitats with an in situ ex-periment,in which constructed forest wetland(FW),natural lake wetland(LW),and lotus pond wetland(LP)were habitats,and plastispheres of virgin and aging low-density polyethylene(LDPE)MPs,as well as surround-ing wetland soils were niches.Significant niche-related differences in bacterial communities were observed,with lower diversity and enrichment of potential plastic-degrading bacteria in the plastisphere than in the soil bacterial communities.Furthermore,habitat-related differences exerted a more pronounced influence on the beta-diversity patterns of the bacterial communities.The linear regression analyses indicated that the local species pool con-tributed more to bacterial community assembly in the LW wetland,whereas the relative abundance of species was the primary factor in the LP wetland.The null model analysis indicated that plastisphere bacterial communi-ties were predominantly driven by the stochastic process,with a more deterministic assembly observed in the LP wetland and soil bacterial communities.Additionally,the primary ecological process shaping plastisphere com-munities shifted from drift in the virgin LDPE to homogenising dispersal in the aging LDPE.This study provides new insights into the fate and ecological impacts of MPs in wetlands,thereby facilitating the effective regulations of plastic pollution.
基金supported by the National Key R&D Program of China(No.2022YFC3203602)the Natural Science Foundation of China(No.52370018)+1 种基金Shaanxi Provincial Youth Innovation Team Project(No.22JP040)Shaanxi Provincial Key Scientific and Technological Innovation Team(No.2023-CX-TD-32).
文摘During the initial impoundment period of a canyon-shaped reservoir,the water body fluctuated violently regarding water level,hydrological condition,and thermal stratification.These variations may alter the structure of phytoplankton community,resulting in algal blooms and seriously threatening the ecological security of the reservoir.It is of great significance to understand the continuous changes of phytoplankton in the initial impoundment period for the protection of reservoir water quality.Therefore,a two-year in-situ monitoring study was conducted on water quality and phytoplankton in a representative canyonshaped reservoir named Sanhekou and the interannual changes of phytoplankton community and its response to environmental changes during the initial impoundment period were discussed at taxonomic versus functional classification levels.The results showed that the total nitrogen and permanganate index levels were relatively high in the first year due to rapid water storage and heavy rainfall input,and the more stable hydrological conditions in the second year promoted the increase of algae density and the transformation of community,and the proportion of cyanobacteria increased significantly.The succession order of phytoplankton in the first year of the initial impoundment periodwas Chlorophyta-Bacillariophyta-Chlorophyta,or J/F/X1-P/MP/W1-A/X1/MP,respectively.And the succession order in the second year was Cyanobacteria/Chlorophyta-Bacillariophyta-Chlorophyta,or L_(M)/G/P-P/A/X1-X1/J/G.Water temperature,relativewater column stability,mixing depth,and pHwere crucial factors affecting phytoplankton community succession.This study revealed the interannual succession law and driving factors of phytoplankton in the initial impoundment period and provided an important reference for the operation management and ecological protection of canyon-shaped reservoirs.
基金supported by Gansu Provincial Science and Technology Plan(23CXGA0195)Longnan Science and Technology Plan(2024CX03)。
文摘Graphene oxide nanomaterials are increasingly used in various fields due to their superior properties.In order to study the influence of graphene oxide additives on the performance of modified asphalt,in this study,graphene oxide modified asphalt was prepared and characteristics was studied including the high deformation resistance performance and the self-healing property of modified asphalt.Functional groups and morphology of graphene oxide modified asphalt were described by Fourier transform infrared spectroscopy.The high deformation resistance performance and self-healing effect of asphalt samples were obtained through dynamic slear rheometer(DSR)test.Results shows that graphene oxide dispersions improve the performance of asphalt relatively well compared to graphene oxide powder.There is no chemical reaction between graphene oxide and asphalt,but physical connection.The addition of graphene oxide improved the high deformation resistance of modified asphalt and expedited the self-healing ability of asphalt under fatigue load.
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund,CAFS(2025XT0902)the earmarked fund for China Agriculture Research System(CARS-46).
文摘This study attempted to assess the lethal concentration(96-h LC_(50))effects of imidacloprid(neonicotinoid pesticide),thiamethoxam(neonicotinoid pesticide),and their combination on juvenile Zebrafish(Danio rerio).Each set of trials contained a control(de-chlorinated tap water),and the experiments were repeated three times.The fish(n=10)were randomly measured with an average length of(3.4±0.34)cm and weight of(1±0.1)g.The temperature was kept at 24℃.Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish(Danio rerio)respectively,whereas experiment 3 was aimed at the combined toxicity of IMI and THM on zebrafish.The tests followed the same study design,and each experiment used seven different logarithmic concentrations of imidacloprid insecticides(310.00,317.08,324.33,331.74,339.32,347.07,355.00 mg/L)and thiamethoxam(175.00,185.52,200.93,215.30,230.70,247.20,264.88 mg/L).The results show that THM is more toxic than IMI,with LC_(50)values of 190.34 mg/L for THM and 310.92 mg/L for IMI.Both individual toxicities showed a substantial positive connection(P<0.05)with confidence limits of 321.50-300.68 mg/L for IMI and 199.91-181.21 mg/L for THM.The joint toxicity test was carried out using the 96-h LC_(50)values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio.The Additive Index(AI)demonstrated that imidacloprid and thiamethoxam acted synergistically on D.rerio.As a matter of fact,more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life.
基金Supported by The Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2025XT0902)Earmarked Fund for China Agriculture Research System(CARS-46).
文摘In this study,the single and jiont acute toxicity effects of pendimethalin(herbicide)and fenitrothion(organophosphate insecticide)were investigated on juvenile zebrafish(Danio rerio)under semi-static conditions.Mortality was assessed at 24,48,72,and 96 h.The study revealed that pendimethalin exhibited higher toxicity than fenitrothion.The 96-h LC 50 values were 0.477 mg/L for pendimethalin and 2.634 mg/L for fenitrothion.Joint exposure produced enhanced toxicity,with 96-h LC 50 values of 0.204 mg/L(pendimethalin equivalent)and 1.139 mg/L(fenitrothion equivalent).Regression analysis showed a significant positive correlation(p<0.05)between pesticide concentration and mortality,while toxicity indices confirmed synergistic interactions.These findings underscore the ecological risks posed by pesticide mixtures and highlight the importance of regulating pesticide use to safeguard aquatic organisms and maintain environmental sustainability.
基金Under the auspices of National Natural Science Foundation of China(No.42101251)。
文摘Flood regulation service(FRS)stands as one of the key benefits that people get from the ecosystem.Under the influence of climate change and human activities,the relationship between supply and demand of FRS would increasingly affect regional flood risk and sustainable development.However,there was currently a lack of systematic study on the future supply-demand relationship of FRS in the flood-vulnerable area undergoing rapidly development in China.This study integrated the Scenario Model Intercomparison Project(ScenarioMIP)with the Shared Socioeconomic Pathways(SSPs)datasets and climate model data to quantify the supply-demand ratio(SDR)of FRS in the Yangtze River Delta(YRD),China from 2020 to 2050.Trend analyses were conducted using linear regres-sion,Theil-Sen median estimation,and Hurst exponent analysis,while key drivers of SDR changes were identified and quantified through the Lindeman-Merenda-Gold(LMG)method between 2021 and 2050.Results show that the supply of FRS in the YRD was generally insufficient to meet the demand.The imbalanced subbasins covered 88.24%of the total study area,with 34.48%of this imbal-anced area concentrated in the Southeastern Basin in China.During 2021 and 2050,the imbalance of FRS supply-demand relationship would largely aggravate in the YRD,of which the aggravated area would account for 77.23%.Under different scenarios,the SDR for FRS would decrease significantly,with rates ranging from-5.45×10^(-4) to-2.06×10^(-4)(P<0.05).Especially,the decline rate of SDR in the YRD Basin(DeltaB)reached 2.92 times that the average of YRD.Human activities were the primary factors that exacerbated the imbalance in FRS supply-demand relationship,of which the relative contribution rate exceeds 75%.Particular attention should be direc-ted toward critical regions like the Southeast Basin in China(SEB)and DeltaB where substantial aggravation of supply-demand imbal-ances of FRS is projected.
基金supported by the National Natural Science Foundation of China(No.41877425)the Shanghai Municipal Natural Science Foundation,China(No.21ZR1446800)+4 种基金the State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil&Water Pollution(No.GHBK-2022-005)the Key Lab of Eco-restoration of Regional Contaminated Environment(Shenyang University)Ministry of Education(No.KF-22-04)the Fundamental Research Funds for the Central Universities(No.2262022-00084)the open fund from the Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration(No.SHUES2022A04)。
文摘In-situ enhanced bioreduction by functional materials is a cost-effective technology to remove chlorinated hydrocarbons in groundwater.Herein,a novel polydopamine(PDA)-modified biochar(BC)-based composite containing nanoscale zero-valent iron(n ZVI)and poly-l-lactic acid(PLLA)(PB-PDA-Fe)was synthesized to enhance the removal of 1,1,1-trichloroethane(1,1,1-TCA)in simulated groundwater with actual site sediments.Its impact on functional microbial community structure in system was also investigated.The typical characterizations revealed uniform dispersion of PLA and n ZVI particles on the BC surface,being smoother after PDA coating.The composite exhibited a significantly higher performance on 1,1,1-TCA removal(82.38%,initial concentration 100 mg/L)than Fe-PDA and PB-PDA treatments.The diversity and richness of the microbial community in the composite treatment consistently decreased during incubation due to a synergistic effect between PLLA-BC and n ZVI.Desulfitobaterium,Pedobacter,Sphaerochaeta,Shewanella,and Clostridium were identified as enriched genera by the composite through DNA-stable isotope probing(DNA-SIP),playing a crucial role in the bioreductive dechlorination process.All the above results demonstrate that this novel composite selectively enhances the activity of microorganisms with extracellular respiration functions to efficiently dechlorinate 1,1,1-TCA.These findings could contribute to understanding the responsive microbial community by carbon-iron composites and expedite the application of in-situ enhanced bioreduction for effective remediation of chlorinated hydrocarbon-contaminated groundwater.
基金supported by the National Key R&D Program of China(2022YFD1900401)。
文摘Exploring the suitability of biochar for improving soil quality under different water and salt conditions is important for maintaining soil health and productivity in the arid regions of northwestern China.We compared the effects of biochar application practices on soil physical,chemical and biological properties under different irrigation and water salinity levels in a two-year field experiment in a mulched and drip-irrigated maize field in Gansu Province,China.Eight treatments in total included the combination of two biochar addition rates of 0 t ha–1(B0)and 60 t ha–1(B1),two irrigation levels of full(W1)and deficit irrigation(W2;W2=1/2 W1)and two water salinity levels of fresh water(S0,0.71 g L–1)and brackish water(S1,4.00 g L–1).The minimum dataset method was used to calculate the soil quality index(SQI)under different treatments.Deficit and brackish water irrigation significantly reduced SQI by 3.80–9.80%through reducing some soil physical,chemical and biological properties.Biochar application significantly increased the SQI by 6.13 and 10.40%under full irrigation with fresh and brackish water,respectively.Biochar addition enhanced the relative abundance of beneficial bacteria(e.g.,Proteobacteria,Patescibacteria)in the soil in all water–salt treatments.The partial least squares path model showed that biochar application significantly enhanced the SQI mainly by improving soil aggregation and pore structure under particular water–salt conditions.This research provides an important basis for utilizing biochar to improve soil quality in arid regions of Northwest China under various water–salt conditions.
基金Supported by The Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2025XT0902)Earmarked Fund for China Agriculture Research System(CARS-46).
文摘The extensive co-occurrence of pyrethroid insecticides such as lambda-cyhalothrin(LCT)and cypermethrin(CPM)in aquatic systems poses a potential risk,yet a significant research gap exists regarding their combined toxicological effects.In this study,the single and joint acute toxicity effects of lambda-cyhalothrin(LCT)and cypermethrin(CPM)on Nile tilapia fingerlings were investigated using 96-h bioassays.Results showed both were highly toxic,with LCT(96-h LC 50=66.53μg/L)being four-fold more potent than CPM(259.41μg/L).Regression analysis confirmed positive correlation(P<0.01)between pesticide concentration and observed mortality.The binary mixture exhibited synergistic effect with Additive Index(AI)>0,indicating combined effects exceeded the sum of their individual actions.This synergism likely stems from mutual inhibition of metabolic detoxification pathways,leading to increased internal concentrations and amplified neurotoxicity.Generally,this study confirmed that single-compound risk assessments dangerously underestimate pyrethroid mixture hazards,necessitating their inclusion in regulatory frameworks for accurate aquatic biodiversity protection.
基金supported by the National Key R&D Program of China(2022YFD1900503).
文摘Carbon storage serves as a key indicator of ecosystem services and plays a vital role in maintaining the global carbon balance.Land use and cover change(LUCC)is one of the primary drivers influencing carbon storage variations in terrestrial ecosystems.Therefore,evaluating the impacts of LUCC on carbon storage is crucial for achieving strategic goals such as the China’s dual carbon goals(including carbon peaking and carbon neutrality).This study focuses on the Aral Irrigation Area in Xinjiang Uygur Autonomous Region,China,to assess the impacts of LUCC on regional carbon storage and their spatiotemporal dynamics.A comprehensive LUCC database from 2000 to 2020 was developed using Landsat satellite imagery and the random forest classification algorithm.The integrated valuation of ecosystem services and trade-offs(InVEST)model was applied to quantify carbon storage and analyze its response to LUCC.Additionally,future LUCC patterns for 2030 were projected under multiple development scenarios using the patch-generating land use simulation(PLUS)model.These future LUCC scenarios were integrated with the InVEST model to simulate carbon storage trends under different land management pathways.Between 2000 and 2020,the dominant land use types in the study area were cropland(area proportion of 35.52%),unused land(34.80%),and orchard land(12.19%).The conversion of unused land and orchard land significantly expanded the area of cropland,which increased by 115,742.55 hm^(2).During this period,total carbon storage and carbon density increased by 7.87×10^(6) Mg C and 20.19 Mg C/hm^(2),respectively.The primary driver of this increase was the conversion of unused land into cropland,accounting for 49.28%of the total carbon storage gain.Carbon storage was notably lower along the northeastern and southeastern edges.By 2030,the projected carbon storage is expected to increase by 0.99×10^(6),1.55×10^(6),and 1.71×10^(6) Mg C under the natural development,cropland protection,and ecological conservation scenarios,respectively.In contrast,under the urban development scenario,carbon storage is projected to decline by 0.40×10^(6) Mg C.In line with China’s dual carbon goals,the ecological conservation scenario emerges as the most effective strategy for enhancing carbon storage.Accordingly,strict enforcement of the cropland red line is recommended.This study provides a valuable scientific foundation for regional ecosystem restoration and sustainable development in arid regions.
基金supported by the National Natural Science Foundation of China(Grant No.41971239)the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0402)+1 种基金Lancang-Mekong Cooperation Project on China-Laos Cross-border Asian Elephant Habitat Quality Assessment(Grant No.102169221100000009022)the Graduate Research Innovation Project of Yunnan University(Grant No.2021Y368).
文摘Human-wildlife conflict(HWC)and its socioeconomic impacts are a pressing global issue.Accurately quantifying HWCs and their interaction with residential development is crucial for rural revitalization and biodiversity conservation efforts.This study investigates the interplay between rural residential expansion(RRE)with humanelephant conflict(HEC)in southern Yunnan Province using high-accuracy yearly land use/land cover data and Asian elephant accident data.A piecewise regression along with several metrics,including expansion intensity,rate of rural residential land,and residential density,were employed to analyze the spatial-temporal change characteristics of RRE.Then,a geographical detector and a bivariate spatial autocorrelation model were used to reveal the driving mechanisms of RRE,with particular emphasis on the spatial relations between RRE and HECs.The results indicate that HECs had a significant negative impact on RRE,exhibiting higher expansion intensity and rate of rural residential land in non-HEC areas than in HEC areas.High spatiotemporal consistency between accelerated RRE and intensified HECs occurred from 2010 onwards,which aligns with the year when the trend of settlement area expansion changed.RRE activities and ensuing land use conversions led to increased occurrences of HECs,which negatively affected the RRE.Compared to HECs,topography and locational factors exhibited a secondary effect on RRE activities.The findings underscore reciprocal feedback mechanisms between RRE and HECs and the elevated risk of adverse interactions between humans and elephants within the range of China’s wild elephants,providing theoretical support for coordinating conservation initiatives for Asian elephants with rural revitalization in the border areas of Southwest China.
基金supported by the following grants,Earmarked fund for CARS-10-Sweet potato,High-quality development of agriculture“5511”collaborative innovation project(XTCXGC2021005)Natural Science Foundation of Fujian province(2021J01495)+1 种基金Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2021R1031008)Science and Technology Innovation Team of Fujian Academy of Agricultural Sciences(CXTD2021012-1).
文摘Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.
基金funded by the Key Technology and System Exploration of Quota Fishing,Ministry of Agriculture and Rural Affairs,Fishery Management Fund Project(No.36,2017)the Zhejiang Fishery Resources Survey Special Project(No.HYS-CZ-202314)。
文摘The red tilefish Branchiostegus japonicus is an economically important demersal fish species with high-quality.However,research on its reproductive biology and population structure characteristics is limited.To better understand the reproductive biological features of B.japonicus,a study was conducted on 461 individuals collected from the East China Sea(27°00′-31°00′N,122°30′-127°30′E)from March to December in 2021.This study analyzed the relationship between standard length and the body weight,and detected the sex ratio,length at first sexual maturity,reproductive period,fecundity,and spawning type of this species.The results revealed a power function relationship between body weight(W)and standard length(L)in the samples as the following regression equation:W=0.000014 L^(3.16)(R^(2)=0.956).The sex ratio was close to 1:1(χ^(2)=1.11,P>0.05),and the sex ratio exhibited significant differences according to different standard lengths.Using the logistic equation,we estimated that 50% of B.japonicus individuals had a standard length at sexual maturity of L_(50)=210 mm.By analyzing the monthly variation in the gonadal index and the proportion of each stage of ovarian development in each month,we assumed that the spawning period of B.japonicus occurred from May to November,and the peak spawning period occurred from June to October.The absolute fecundity was 51441±33232 eggs,the relative fecundity at the standard length was(210±117)eggs mm-1,and the relative fecundity relative to body weight was(184±89)eggs g^(-1).The distribution of the egg diameter of B.japonicus had only one obvious peak,indicating the spawning pattern of B.japonicus was once a year.This study updated and enriched the basic biological data of B.japonicus,which is beneficial for obtaining a better understanding of its population dynamics,and promoting the conservation and sustainable utilization of B.japonicus in East China Sea.
基金supported by the Open Fund of the Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry of Agriculture and Rural Affairsof China(KFJJ202101)the National KeyR&D Program of China(2021YFD1400100)+1 种基金the National Natural Science Foundation of China(31972247)the Tianchi Talent Introduction Program in Xinjiang Uygur Autonomous Region,China and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
文摘The sugar beet cyst nematode(Heterodera schachtii) is one of the most destructive pathogens in sugar beet production, which causes serious economic losses every year. Few molecular details of effectors of H. schachtii parasitism are known. We analyzed the genome and transcriptome data of H. schachtii and identified multiple potential predicted proteins. After filtering out predicted proteins with high homology to other plant-parasitic nematodes, we performed functional validation of the remaining effector proteins. 37 putative effectors of H. schachtii were screened based on the Nicotiana benthamiana system for identifying the effectors that inhibit plant immune response, eventually determines 13 candidate effectors could inhibit cell death caused by Bax. Among the 13 effectors, nine have the ability to inhibit GPA2/RBP1-induced cell death. All 13 effectortriggered immunity(ETI) suppressor genes were analyzed by qRT-PCR and confirmed to result in a significant downregulation of one or more defense genes during infection compared to empty vector. For in situ hybridization,13 effectors were specifically expressed and located in esophageal gland cells. These data and functional analysis set the stage for further studies on the interaction of H. schachtii with host and H. schachtii parasitic control.
基金funded by the National Key R&D Program of China(2022YFD1300803)the China Agriculture Research System of MOF and MARA(CARS-34)。
文摘Grain soybean(Glycine max(L.)Merr.)shows potential as a forage crop following its introduction from low to high latitudes due to in its growth phenotype.However,the application of this approach is impeded by the diverse ecological types of soybean,their adaptability to the introduction,and the uncertainty surrounding the growth phenotype post-introduction.This study introduced 24 grain soybean varieties from low-latitude regions(22-31°N)to the high-latitude northwestern Loess Plateau(39°N)between 2018 and 2019.The growth phenotypes were observed,and their forage productivity potential was assessed.All varieties displayed delayed flowering following cross-latitude introduction,with the vegetative growth phase of some varieties even exceeding their entire growth duration at their origin.Rapid dry matter accumulation rates indicated growth adaptation performance and formed the basis for soybean forage yield.Varieties from the tropical South China region exhibited significant yield advantages,with dry matter yields of 8.97-14.68 t ha^(-1)and crude protein yields of 1.44-2.51 t ha^(-1).Varieties HX3 and GX7 from this region demonstrated optimal growth adaptability and productivity in the cross-latitude environment,achieving the highest dry matter yields of 14.68 and 13.86 t ha^(-1),respectively.As a result,HX3 and GX7 are recommended for local farming systems to provide high-quality forage.The cross-latitude introduction of soybean is proposed as a viable and efficient strategy for forage improvement and application.
基金project is supported in part by Broadband Seismic 3D Array Detection(PhaseⅠ),Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project(Grant No.2024ZD1000300)National Natural Science Foundation of China(42204061)Gansu Jishishan 6.2 magnitude earthquake scientific investigation(DQJB23Y45)program。
文摘Based on the observational data from 60 short-period stations deployed in the Jishishan M6.2 earthquake epicenter and adjacent regions(Gansu Province,2023),this study inverted the near-surface S-wave velocity structure through teleseismic receiver function analysis by using the amplitude of direct P-wave.The results reveal that the epicentral area(Liugou Township and surroundings)exhibits markedly low S-wave velocities of 400-600 m/s,with a mean value of(500±50)m/s.In contrast,intermountain basins-Guanting Basin and Dahejia Basin-demonstrate significantly elevated velocities,exceeding the epicentral zone by 100-300 m/s,with values concentrated at 600-900 m/s.Notably,localized areas such as Jintian Village and Caotan Village maintain stable S-wave velocities of(700±30)m/s.The western margin tectonic belt of Jishishan displays distinctive velocity differentiation:A pronounced velocity gradient zone along the 35.8°N latitude boundary separates northern areas(<550 m/s)from southern regions(>750 m/s).These findings demonstrate significant spatial heterogeneity in shallow S-wave velocity structures,primarily controlled by three factors:(1)topographic-geomorphic units,(2)stratigraphic lithological contrasts,and(3)anthropogenic modifications.The persistent low-velocity anomalies(<600 m/s)in the epicentral zone and northern Yellow River T2 terrace likely correlate with Quaternary unconsolidated sediments,enhanced groundwater circulation,and bedrock weathering.These results provide critical geophysical constraints for understanding both the seismogenic environment of the Jishishan earthquake and its damage distribution patterns.Furthermore,they establish a foundational framework for regional seismic intensity evaluation,site amplification analysis,and secondary hazard risk assessment.
基金supported by the National Natural Science Foundation of China(Nos.32271726 and 32171648)the Natural Science Foundation of Hubei Province of China(No.2022CFB030)。
文摘Soil denitrification,anammox,and Feammox are key for nitrogen(N)removal in agriculture.Despite potassium(K)fertilizer enhancing N efficiency,their role in regulation of these processes is unclear.A soil column incubation with 15N isotope tracingwas conducted to explore millimeter-scale interactions of N and K on these pathways in soil fertilization zones.After 28 days,individual applications of N and K reduced denitrification-nitrogen removal rate(DNRR),anammox-nitrogen removal rate(ANRR),and feammox-nitrogen removal rate(FNRR)compared to a non-fertilizer control.N fertilizer had a greater effect than K,likely due to the high consumption of dissolved organic carbon by N fertilizer or the increased soil organic matter decomposition by K fertilizer.Combing of N and K increased DNRR,ANRR and FNRR rates by 31%,3090%and 244%compared to single N,and by-53.7%,885%and 222%compared to single K.These effects diminished with depth and distance from fertilizer sites.The effects of N fertilizer on these N removal processes might be regulate abundance of key microbes(e.g.,Limnobacter and Clostridium)and key gene(nirK,hzsB,ACM and Geo)by providing N substrates,while K enhances N metabolism efficiency through enzyme activation,indicated by the downregulation of certain genes(hzsB,ACM and Geo)and a negative correlation with N removal by simultaneously increasing gene expression and enzyme activity.These findings provide insights into how N and K together enhance N removal,emphasizing their importance for optimizing this process.
基金funded by the Open Research Fund of TPESER(Grant No.TPESER202502)the National Key Research and Development Program of China(Grant No.2024YFF0810700)+3 种基金the Science and Technology Program Project of Gansu Province,China(Grant No.25JRRA138)the Hydraulic Science Experimental Research and Technology Extension Project of Gansu Province,China(Grant No.25GSLK094)the Open Foundation of MOE Key Laboratory of Western China's Environmental System,Lanzhou Universitythe Fundamental Research Funds for the Central Universities(Grant No.1zujbky-2025-jdzx02)。
文摘Surge-type glaciers are widely developed in mountainous areas around the world.Understanding the trigger mechanism of glacier surge is a prerequisite for addressing their impacts on hydrological assessments,disentangling climate-glacier linkages,and mitigating downstream hazards.Most glacier surges occur in the compound glaciers;however,attention paid to the trigger mechanisms of such surges is minimal.This study confirmed two surges in the northern and southern branches of the Aru-4 glacier,respectively,in the Western Tibetan Plateau,using multisource remote sensing data.The northern branch of the Aru-4 glacier entered the active phase in 1999 and the active phase lasted for 6 years.The southern branch of the Aru-4 glacier entered the active phase in 2007 and the active phase lasted for 9 years.The southern branch of the Aru-4 glacier experienced a long period of retreat before the northern branch surged and their tongues were in a detached state.The northern branch surge carried a large amount of ice to the frontal area,blocking the downward transport of ice from the southern branch and initiated surge.Through the analysis of two surge processes of Aru-4 glacier,we found a new surge mechanism for compound glaciers.It was revealed that surges in such glaciers are not only triggered by the reduction in basal sliding resistance caused by the internal factors.These surges initiated in the upper part of the glacier then propagated to down glacier by intense compression force.Furthermore,surges can also be triggered by external intervention of blocking by other branches.This external trigger initiates the surge in the lower part of the glacier then propagated to the upper part by longitudinal traction force.In addition,comparing with the surge triggered by the internal factors,the surge triggered by the external intervention may have a more dramatic process.
基金The National Key R&D Program of China under contract No. 2022YFE0209300National Natural Science Foundation of China under contract No. 42176091+1 种基金Asia Cooperation Fund of China (Comparative Study of Geoenvironment and Geohazards in the Yangtze River Delta and the Red River Delta)China Geological Survey Project under contract No. DD20242714。
文摘This study investigates the spatial distribution and regulatory mechanisms of dissolved inorganic carbon(DIC) in Xiangshan Bay, East China Sea, addressing critical gaps in carbon cycling research within semi-enclosed bays. Through isotopic analysis(δ^(13)C_(DIC), δD and δ^(18)O) and hydrochemical measurements [salinity and dissolved oxygen(DO)] of surface and bottom seawater samples from 52 stations, we demonstrate that δ^(13)C_(DIC) values(surface:-3.6‰ to-2.1‰;bottom:-3.6‰ to-1.8‰) exhibit distinct vertical and spatial patterns, with higher values in surface waters and outer bay regions compared to bottom and inner bay areas. Conservative mixing between seawater(average contribution:56%) and freshwater dominates DIC dynamics, while tidal hydrodynamics amplify the imprint of riverine inputs during low tides. Nutrient gradients driven by saline-freshwater mixing enhance primary productivity in outer bay regions,resulting in 13C-enriched DIC and elevated dissolved organic carbon(DOC) concentrations. Conversely, bottom waters show 13C-depleted signatures(-2.75‰ mean δ^(13)C_(DIC)), reflecting organic matter degradation under oxygen-depleted conditions. Aquaculture activities exacerbate localized eutrophication, with monsoon-enhanced runoff amplifying anthropogenic impacts. This work underscores the sensitivity of coastal carbon cycling to both natural hydrodynamics and anthropogenic perturbations in semi-enclosed bays.
基金supported by the National Ecological Environment Survey and Assessment(2024-vertical-0107)the Fundamental Research Funds for the Central Public-interest Scientific Institution(2023YSKY-26)the Hulun Buir Grassland Ecological Restoration Comprehensive Survey Project(DD20230474).
文摘In recent years,intensive human activities have increased the intensity of desertification,driving continual desertification process of peripheral meadows.To investigate the effects of restoration on soil microbial communities,we analyzed vegetation-soil relationships in the Hulun Buir Sandy Land,northern China.Through the use of high-throughput sequencing,we examined the structure and diversity in the bacterial and fungal communities within the 0-20 cm soil layer after 9-15 a of restoration.Different slope positions were analyzed and spatial heterogeneity was assessed.The results showed progressive improvements in soil properties and vegetation with the increase of restoration duration,and the following order was as follows:bottom slope>middle slope>crest slope.During the restoration in the Hulun Buir Sandy Land,the bacterial communities were dominated by Proteobacteria,Actinobacteria,and Acidobacteria,whereas the fungal communities were dominated by Ascomycota and Basidiomycota.Eutrophic bacterial abundance increased with the restoration duration,whereas oligotrophic bacterial and fungal abundance levels decreased.The soil bacterial abundance significantly increased with the increasing restoration duration,whereas the fungal diversity decreased after 11 a of restoration,except that at the crest slope.Redundancy analysis showed that pH,soil moisture content,total nitrogen,and vegetation-related factors affected the bacterial community structure(45.43%of the total variance explained).Canonical correspondence analysis indicated that pH,total phosphorus,and vegetation-related factors shaped the bacterial community structure(31.82%of the total variance explained).Structural equation modeling highlighted greater bacterial responses(R^(2)=0.49-0.79)to changes in environmental factors than those of fungi(R^(2)=0.20-0.48).The soil bacterial community was driven mainly by pH,soil moisture content,electrical conductivity,plant coverage,and litter dry weight.The abundance and diversity of the soil fungal community were mainly driven by plant coverage,litter dry weight,and herbaceous aboveground biomass,while there was no significant correlation between the soil fungal community structure and environmental factors.These findings highlighted divergent microbial succession patterns and environmental sensitivities during sandy grassland restoration.
