According to practice and exploration of spark program for 26 years in Quanzhou,the main model and their effects of new rural construction in west bank of Taiwan Strait led by spark science and technology were expound...According to practice and exploration of spark program for 26 years in Quanzhou,the main model and their effects of new rural construction in west bank of Taiwan Strait led by spark science and technology were expounded. Six spark program systems were established,consisting of policy support guide,science and technology project lead,experts' intelligence support,spark science and technology training,scitech information service and spark program demonstration. Five spark projects were implemented to promote new rural construction in the west bank of Taiwan Strait,such as constructing the national spark industrial zone,organizing the new rural construction led by science and technology,constituting spark sci-tech innovation center,developing the rural informatization,and establishing science and technology commissioner bases. Finally,enlightenment of spark program and its development ways in the future were put forward.展开更多
InP quantum dots(QDs)have been a major building block of modern display technology due to their high photoluminescence quantum yield(PLQY)in the visible spectrum,superior stability,and eco-friendly composition.However...InP quantum dots(QDs)have been a major building block of modern display technology due to their high photoluminescence quantum yield(PLQY)in the visible spectrum,superior stability,and eco-friendly composition.However,their applications at short-wave infrared(SWIR)have been hindered by their low efficiency.Here,we report the synthesis of efficient and SWIR-emitting InP QDs by precisely controlling the InP core nucleation using a low-cost ammonia phosphorus precursor,while avoiding size-limiting ZnCl_(2) for effective copper doping.Subsequent epitaxial growth of a lattice-matched ZnSe/ZnS multishell enhanced the QD sphericity and surface smoothness and yielded a record PLQY of 66% with an emission peak at 960 nm.When QDs were integrated as the high-refractive-index luminescent core of a liquid waveguide-based luminescent solar concentrator(LSC),the device achieved an optical efficiency of 7.36%.This performance arises from their high PLQY,spectral alignment with the responsivity peak of silicon solar cells,and the optimized core/cladding waveguide structure.These results highlight the potential of InP QDs as a promising nanomaterial for SWIR emission and applications.展开更多
Excessive levels of Fluoride(F−)and Cadmium(Cd)in drinking groundwater may pose health risks.This study assessed the health risks associated with F−and Cd contamination in rural drinking groundwater sources in Wutai C...Excessive levels of Fluoride(F−)and Cadmium(Cd)in drinking groundwater may pose health risks.This study assessed the health risks associated with F−and Cd contamination in rural drinking groundwater sources in Wutai County,Shanxi Province,China,to support population health protection,water resource management,and environmental decision-making.Groundwater samples were collected and analyzed,and a Human Health Risk Model(HHRA)was applied to evaluate groundwater quality.The results showed that both contents of F−and Cd in groundwater exceeded the Class III limits of China's national groundwater quality standard(GB/T 14848—2024).Fluoride levels met the Class V threshold,with enrichment area mainly located in the east part of the study area.Cadmium levels reached Class IV,with elevated concentrations primarily observed in the western and northwestern regions.Correlation analysis revealed that F−showed weak or no correlation with other measured substances,indicating independent sources.Health risk assessment results indicated that F−poses potential health risks to rural residents,while cadmium,due to its relatively low concentrations,does not currently present a significant health risk.Among different demographic groups,the health risk levels of F−exposure followed the order:Infants>children>adult females>adult males.The findings highlight that fluoride is the primary contributor to health risks associated with groundwater consumption in the study area.Strengthened monitoring and prevention of F−contamination are urgently needed.This research provides a scientific basis for the prevention and control of fluoride pollution in groundwater and offers practical guidance for safeguarding drinking water safety in rural China.展开更多
President Xi,focusing on building a new pattern of all-round opening up to the outside world and promoting the common prosperity and progress of all countries,put forward a major proposal for the construction of the S...President Xi,focusing on building a new pattern of all-round opening up to the outside world and promoting the common prosperity and progress of all countries,put forward a major proposal for the construction of the Silk Road Economic Belt and the maritime Silk Road in twenty-first Century.The bay area economy as an important coastal economic form,is the highlight of the current international economic map,and is a significant symbol of the world's first-class coastal city.The international first-class bay areas,such as New York Bay area,San Francisco Bay area and Tokyo Bay area,are characterized by openness,innovation,livability and internationalization.The Guangdong-Hong Kong-Macao Greater Bay Area has four worldclass cities,name Guangzhou,Shenzhen,Hong Kong and Macao,and the Pearl River Delta city cluster.It has an open economic structure,efficient resource allocation ability,strong agglomeration and spillover function and development international communication network plays a core function of leading innovation and gathering radiation.It is an important growth pole for promoting the economic development of the maritime Silk Road and a leader in technological change.With the help of Portugal's geographical position on the maritime Silk Road and market access advantages within the EU system,the Greater Bay Area should further deepen scientific and technological cooperation,improve the ability of scientific and technological innovation of both sides,let the scientific research subjects of the bay area help Portugal's scientific and technological development,and enhance PortugaFs scientific and technological position in the EU.From the perspective of the Belt and Road Initiative,combined with the basic situation and cooperation of China,especially Guangdong,Hong Kong and Macao,and the development of science and technology in Portugal,this article explores the technological fields,policy measures that are suitable for scientific and technological cooperation between the two sides,and puts forward corresponding suggestions to contribute to the technological development of China,Portugal and the global economic and social sustainable development.展开更多
Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has...Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.展开更多
Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asia...Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asian,especially in Chinese.To update the genotypes of PH1 in the Chinese population,we collected and identified 7 Chinese probands with PH1 from 2013 to 2017 in our center,five of whom had delayed diagnosis and failed in kidney transplantation.Samples of peripheral blood DNA from the 7 patients and their family members were collected and sequencing analysis was performed to test the mutations of gene AGXT.Western blotting and enzyme activity analysis were conducted to evaluate the function of the mutations.Furthermore,a systematic review from 1998 to 2017 was performed to observe the genetic characteristics between Chinese and Caucasian. The results showed that a total of 12 mutations were identified in the 7 pedigrees.To the best of ourknowledge,2 novel variants of A GXT,p.Gly41 Trp and p.Leu33Met,were first reported.Bioinformatics and functional analysis showed that only 7 mutations led to a reduced expression of alanine-glyoxylate amino transferase (AGT)at a protein level.The systematic review revealed significant population heterogeneity in PH1.In conclusion,new genetic subtypes and genetic characteristics of PH1 are updated in the Chinese population. Furthermore,a genotype-phenotype correlation is found in PH1.展开更多
Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain met...Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.展开更多
Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship b...Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization,energy metabolism,and gut microbiota in growing pigs.Methods Thirty-six growing barrows(47.2±1.5 kg)were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3β-glucan-to-arabinoxylan ratios.In the experiment,nutrient utilization,energy metabolism,fecal microbial community,and production and absorption of short-chain fatty acid(SCFA)of pigs were investigated.In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut.Results The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber.In animal experiments,increasing the dietary apparent viscosity and theβ-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility(AID),apparent total tract digestibility(ATTD),and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter(P<0.05).In addition,increasing dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased gas exchange,heat production,and protein oxidation,and decreased energy deposition(P<0.05).The dietary apparent viscosity andβ-glucanto-arabinoxylan ratios had linear interaction effects on the digestible energy,metabolizable energy,retained energy(RE),and net energy(NE)of the diets(P<0.05).At the same time,the increase of dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased SCFA production and absorption(P<0.05).Increasing the dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria(P<0.05)and the relative abundance of beneficial bacteria.Furthermore,increasing the dietaryβ-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta(P<0.001).Finally,the prediction equations for RE and NE were established.Conclusion Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization,energy metabolism,and pig gut microbiota composition and metabolites.展开更多
Timely and accurate forecasting of storm surges can effectively prevent typhoon storm surges from causing large economic losses and casualties in coastal areas.At present,numerical model forecasting consumes too many ...Timely and accurate forecasting of storm surges can effectively prevent typhoon storm surges from causing large economic losses and casualties in coastal areas.At present,numerical model forecasting consumes too many resources and takes too long to compute,while neural network forecasting lacks regional data to train regional forecasting models.In this study,we used the DUAL wind model to build typhoon wind fields,and constructed a typhoon database of 75 processes in the northern South China Sea using the coupled Advanced Circulation-Simulating Waves Nearshore(ADCIRC-SWAN)model.Then,a neural network with a Res-U-Net structure was trained using the typhoon database to forecast the typhoon processes in the validation dataset,and an excellent storm surge forecasting effect was achieved in the Pearl River Estuary region.The storm surge forecasting effect of stronger typhoons was improved by adding a branch structure and transfer learning.展开更多
Recent advances in earth science and exploration have made deepwater channel-levee systems a research focus.We collected and analyzed over 10000 km of two-dimensional multichannel seismic data from the offshore Indus ...Recent advances in earth science and exploration have made deepwater channel-levee systems a research focus.We collected and analyzed over 10000 km of two-dimensional multichannel seismic data from the offshore Indus Basin to identify channellevee systems at various hierarchical levels depending on their seismic reflection characteristics.Seismic facies analysis was integrated with well data to map the spatial distribution of channel-levee systems in the offshore Indus Basin across various geological periods,and the factors influencing their development were discussed.These systems within the basin were identified using a developed,refined three-tier classification method.The first-order system consists of multiple spatially stacked complexes,the second-order system continuously developed multistage channel-levee bodies,and the third-order system represents the smallest identifiable sedimentary units on seismic profiles.Our findings demonstrate the evolution of the offshore Indus Basin from a single-stage channel with lateral migration to multistage vertical channel stacking from the Miocene to the Pleistocene.Tectonic activities exert their effect on channel-levee systems through their influence on the relative sea level.They also trigger volcanic or seismic events and affect siliciclastic supply.Warm and humid climate conditions form large river systems,which aid in the transport of terrestrial debris to the basin margin.Most channel-levee systems are assumed to have formed during low sea-level periods.This study offers new insights into the formation and evolution of turbidite sedimentary systems in the offshore Indus Basin and presents a practical classification method for comprehending gravity-flow sedimentary configurations and deepwater hydrocarbon exploration.展开更多
Low–angle submarine landslides pose a greater threat to offshore infrastructure compared to those with steep sliding angles.Understanding the preparation and triggering mechanism of these low–angle submarine landsli...Low–angle submarine landslides pose a greater threat to offshore infrastructure compared to those with steep sliding angles.Understanding the preparation and triggering mechanism of these low–angle submarine landslides remains a significant challenge.This study focuses on a deformed low–angle submarine landslide in the shelf–slope break of the Pearl River Mouth Basin,South China Sea,integrating sedimentology,geophysics,and geotechnology to investigate potential failure mechanisms.The architecture and deformation characteristics of the submarine landslide were elucidated by analyzing multibeam and seismic data.Within the context of the regional geological history and tectonic framework,this study focuses on the factors(e.g.,rapid sedimentation,fluid activity,and earthquakes)that potentially contributed to the submarine slope failure.Furthermore,a series of stability evaluations considering the effects of rapid sedimentation and earthquakes was conducted.Our findings indicate that the most probable triggering mechanism involves the combined effects of sedimentation controlled by sea–level fluctuations,high–pressure gas activity,and seismic events.The high–pressure gas,which acts as a long–term preconditioning factor by elevating pore pressures and reducing shear resistance within the sediment,accumulated beneath the upper and middle sections of the low–permeability stratum that was formed during sea–level rise and ultimately evolved into the sliding mass.The overpressure generated by gas accumulation predisposed the submarine slope to instability,and a frequent or moderate earthquake ultimately initiated local failure.This study enhances the mechanistic understanding of low–angle slope failures in the shelf–slope break zone and provides critical insights for assessing marine hazard risks.展开更多
The Indonesian Throughflow(ITF)plays important roles in global ocean circulation and climate systems.Previous studies suggested the ITF interannual variability is driven by both the El Niño-Southern Oscillation(E...The Indonesian Throughflow(ITF)plays important roles in global ocean circulation and climate systems.Previous studies suggested the ITF interannual variability is driven by both the El Niño-Southern Oscillation(ENSO)and the Indian Ocean Dipole(IOD)events.The detailed processes of ENSO and/or IOD induced anomalies impacting on the ITF,however,are still not clear.In this study,this issue is investigated through causal relation,statistical,and dynamical analyses based on satellite observation.The results show that the driven mechanisms of ENSO on the ITF include two aspects.Firstly,the ENSO related wind field anomalies driven anomalous cyclonic ocean circulation in the western Pacific,and off equatorial upwelling Rossby waves propagating westward to arrive at the western boundary of the Pacific,both tend to induce negative sea surface height anomalies(SSHA)in the western Pacific,favoring ITF reduction since the develop of the El Niño through the following year.Secondly,the ENSO events modulate equatorial Indian Ocean zonal winds through Walker Circulation,which in turn trigger eastward propagating upwelling Kelvin waves and westward propagating downwelling Rossby waves.The Rossby waves are reflected into downwelling Kelvin waves,which then propagate eastward along the equator and the Sumatra-Java coast in the Indian Ocean.As a result,the wave dynamics tend to generate negative(positive)SSHA in the eastern Indian Ocean,and thus enhance(reduce)the ITF transport with time lag of 0-6 months(9-12 months),respectively.Under the IOD condition,the wave dynamics also tend to enhance the ITF in the positive IOD year,and reduce the ITF in the following year.展开更多
Solar activity plays an important role in influencing space weather,making it important to understand numerous aspects of spatial and temporal variations in the Sun's radiative output.High-performance deep learnin...Solar activity plays an important role in influencing space weather,making it important to understand numerous aspects of spatial and temporal variations in the Sun's radiative output.High-performance deep learning models and long-term observational records of sunspot relative numbers are essential for solar cycle forecasting.Using the multivariate time series of monthly sunspot relative numbers provided by the National Astronomical Observatory of Japan and two Informer-based models,we forecast the amplitude and timing of solar cycles 25 and 26.The main results are as follows:(1)The maximum amplitude of solar cycle 25 is higher than the previous solar cycle 24 and the following solar cycle 26,suggesting that the long-term oscillatory variation of sunspot magnetic fields is related to the roughly centennial Gleissberg cyclicity.(2)Solar cycles 25 and 26 exhibit a pronounced Gnevyshev gap,which might be caused by two non-coincident peaks resulting from solar magnetic flux transported by meridional circulation and mid-latitude diffusion in the convection zone.(3)Hemispheric prediction of sunspot activity reveals a significant northsouth asynchrony,with activity level of the Sun being more intense in the southern hemisphere.These results are consistent with expectations derived from precursor methods and dynamo theories,and further provide evidence for internal changes in solar magnetic field during the decay of the Modern Maximum.展开更多
Ferroptosis is a form of cell death elicited by an imbalance in intracellular iron concentrations,leading to enhanced lipid peroxidation.In neurological disorders,both oxidative stress and mitochondrial damage can con...Ferroptosis is a form of cell death elicited by an imbalance in intracellular iron concentrations,leading to enhanced lipid peroxidation.In neurological disorders,both oxidative stress and mitochondrial damage can contribute to ferroptosis,resulting in nerve cell dysfunction and death.The ubiquitin-proteasome system(UPS)refers to a cellular pathway in which specific proteins are tagged with ubiquitin for recognition and degradation by the proteasome.In neuro-logical conditions,the UPS plays a significant role in regu-lating ferroptosis.In this review,we outline how the UPS regulates iron metabolism,ferroptosis,and their interplay in neurological diseases.In addition,we discuss the future application of small-molecule inhibitors and identify poten-tial drug targets.Further investigation into the mechanisms of UPS-mediated ferroptosis will provide novel insights and strategies for therapeutic interventions and clinical applica-tions in neurological diseases.展开更多
Ferroptosis,a type of programmed cell death,represents a distinct paradigm in cell biology.It is characterized by the iron-dependent accumulation of reactive oxygen species,which induce lipid peroxidation(LPO),and is ...Ferroptosis,a type of programmed cell death,represents a distinct paradigm in cell biology.It is characterized by the iron-dependent accumulation of reactive oxygen species,which induce lipid peroxidation(LPO),and is orchestrated by the interplay between iron,lipid peroxides,and glutathione.In this review,we emphasize the frequently overlooked role of iron in LPO beyond the classical iron-driven Fenton reaction in several crucial processes that regulate cellular iron homeostasis,including iron intake and export as well as ferritinophagy,and the emerging roles of endoplasmic reticulum-resident flavoprotein oxidoreductases,especially P450 oxidoreductases,in modulating LPO.We summarize how various types of fatty acids(FAs),including saturated,monounsaturated,and polyunsaturated FAs,differentially influence ferroptosis when incorporated into phospholipids.Furthermore,we highlight the therapeutic potential of targeting LPO to mitigate ferroptosis and discuss the regulatory mechanisms of endogenous lipophilic radical-trapping antioxidants that confer resistance to ferroptosis,shedding light on therapeutic avenues for ferroptosis-associated diseases.展开更多
Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating ...Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating excitationcontraction(E-C)coupling.Mutations in JPH2 have been associated with hypertrophic cardiomyopathy(HCM),but the molecular mechanisms governing its membrane-binding properties and the functional relevance of its membrane occupation and recognition nexus(MORN)repeat motifs remain incompletely understood.This study aimed to elucidate the structural basis of JPH2 membrane association and its implications for HCM pathogenesis.Methods A recombinant N-terminal fragment of mouse JPH2(residues 1-440),encompassing the MORN repeats and an adjacent helical region,was purified under near-physiological buffer conditions.X-ray crystallography was employed to determine the structure of the JPH2 MORN-Helix domain.Sequence conservation analysis across species and junctophilin isoforms was performed to assess the evolutionary conservation of key structural features.Functional membrane-binding assays were conducted using liposome co-sedimentation and cell-based localization studies in COS7 and HeLa cells.In addition,site-directed mutagenesis targeting positively charged residues and known HCM-associated mutations,including R347C,was used to evaluate their effects on membrane interaction and subcellular localization.Results The crystal structure of the mouse JPH2 MORN-Helix domain was resolved at 2.6Å,revealing a compact,elongated architecture consisting of multiple tandem MORN motifs arranged in a curved configuration,forming a continuous hydrophobic core stabilized by alternating aromatic residues.A C-terminalα-helix further reinforced structural integrity.Conservation analysis identified the inner groove of the MORN array as a highly conserved surface,suggesting its role as a protein-binding interface.A flexible linker segment enriched in positively charged residues,located adjacent to the MORN motifs,was found to mediate direct electrostatic interactions with negatively charged phospholipid membranes.Functional assays demonstrated that mutation of these basic residues impaired membrane association,while the HCM-linked R347C mutation completely abolished membrane localization in cellular assays,despite preserving the overall MORN-Helix fold in structural modeling.Conclusion This study provides structural insight into the membrane-binding mechanism of the cardiomyocyte-specific protein JPH2,highlighting the dual roles of its MORN-Helix domain in membrane anchoring and protein interactions.The findings clarify the structural basis for membrane targeting via a positively charged linker and demonstrate that disruption of this interaction—such as that caused by the R347C mutation—likely contributes to HCM pathogenesis.These results not only enhance current understanding of JPH2 function in cardiac E-C coupling but also offer a structural framework for future investigations into the assembly and regulation of JMCs in both physiological and disease contexts.展开更多
Apoptosis preserves organismal homeostasis by selectively eliminating unnecessary or damaged cells, with accumulating evidence also suggesting that it activates regenerative pathways and facilitates tissue remodeling....Apoptosis preserves organismal homeostasis by selectively eliminating unnecessary or damaged cells, with accumulating evidence also suggesting that it activates regenerative pathways and facilitates tissue remodeling. To date, however, the regulatory mechanisms linking this form of programmed cell death to regeneration remain poorly defined, particularly in evolutionarily basal organisms. Using the sea cucumber (Apostichopus japonicus) as a model for intestinal regeneration, this study identified robust apoptotic activity across key regenerative stages. Pharmacological suppression of apoptosis during wound healing and mesenteric scaffold formation critically impaired intestinal regeneration. Quantitative proteomics using direct data-independent acquisition (DIA) revealed coordinated down-regulation of lipid metabolic pathways under apoptosis-inhibited conditions, with notable suppression of Ca^(2+)-independent phospholipase A2 (iPLA2), an enzyme typically up-regulated during successful regeneration. In parallel, expression of regeneration-associated factors WNT6 and EGFL7 was markedly reduced under apoptotic blockade. Targeted inhibition of iPLA2, EGFL7, or WNT6 each resulted in impaired mesenteric outgrowth and reduced proliferative activity within the regenerating intestinal primordia. Collectively, these findings suggest two potential mechanistic pathways: apoptosis-mediated regeneration of lipid metabolism via iPLA2 and apoptosis-dependent activation of WNT6/EGFL7 signaling. This study provides mechanistic insight into apoptosis-coupled regenerative processes in basal deuterostomes and expands the conceptual framework of programmed cell death in tissue renewal.展开更多
Blooms of the toxic dinoflagellate Alexandrium spp.,which produce paralytic shellfish toxins(PSTs),have threatened marine ecosystems for decades.However,their impacts on benthic copepods remain underexplored.Here,we i...Blooms of the toxic dinoflagellate Alexandrium spp.,which produce paralytic shellfish toxins(PSTs),have threatened marine ecosystems for decades.However,their impacts on benthic copepods remain underexplored.Here,we investigated the physiological and biochemical responses of marine benthic copepod Tigriopus japonicus to Alexandrium pacificum.Adult female showed no mortality within 24 h and maintained high survival(>80%)across all A.pacificum concentrations over 96 h,with no significant difference from non-toxic controls.Ingestion rates on A.pacificum and non-toxic controls were similar,when algal prey concentrations were lower than about 2500µgC/L.However,above this threshold,feeding of T.japonicus was significantly depressed by A.pacificum.Six-day exposure induced oxidative stress,marked by elevated level of reactive oxygen species,malondialdehyde,total antioxidant capacity and superoxide dismutase activity,while catalase activity showed a non-significant increase.Moreover,concentration-dependent increase was observed in acetylcholinesterase activity,and its positive correlation with oxidative biomarkers suggests additional roles in coping with stress.Chronic exposure over three generations did not consistently affect development,except for complete development failure in F2 and F3 at the highest concentration.However,moderate-to-high concentrations of A.pacificum significantly impaired reproduction,reducing egg production by 9.66%–96.07%and clutch numbers by 33.33%–55.56%.Our findings indicate that Alexandrium blooms can adversely impact benthic copepod feeding and population recruitment through neurotoxicity and oxidative stress,highlighting the ecological risks of prolonged algal blooms in benthic habitats.展开更多
Recently,allotriploids from Pacific oysters(Crassostrea gigas)and Fujian oysters(C.angulata)have been grown for aquaculture.However,the viability of these allotriploids remains uncertain.In this study,two autotriploid...Recently,allotriploids from Pacific oysters(Crassostrea gigas)and Fujian oysters(C.angulata)have been grown for aquaculture.However,the viability of these allotriploids remains uncertain.In this study,two autotriploids,TGG(diploid C.gigas♀×tetraploid C.gigas♂)and TAA(diploid C.angulata♀×tetraploid C.angulata♂),and two allotriploids,TGA(diploid C.gigas♀×tetraploid C.angulata♂)and TAG(diploid C.angulata♀×tetraploid C.gigas♂),were studied to assess the heterosis in growth and survival at different temperatures and salinities.The results showed that during the larval stage,TAG exhibited increased heterosis in growth and survival when temperature rose.During the adult stage,the growth of TGA significantly outperformed other triploids at higher temperatures(23 and 28℃)and salinities(25 and 30).In contrast,TAG demonstrated the highest survival probability across all conditions except at salinity 25,where it equaled TAA after day 19.The highest oxygen consumption rates(OCR)of TGA and TAG were observed at 23 and 28℃,while the ammonia excretion rate(AER)of TAG was significantly higher than that of TGA at 23℃.These physiological parameters reflect the advantage of TAG in terms of survival.Notably,the temperature coefficient of allotriploids was higher than that of autotriploids in the range of 18–23℃.At 28℃,TAG showed the highest superoxide dismutase(SOD)and catalase(CAT)activities and the lowest malondialdehyde(MDA)content,showing its advantage when encountering high-temperature.展开更多
The study of back-arc sedimentary basins is key to understanding oceanic and continental geodynamic processes and mechanisms.The East China Sea Shelf Basin(ECSSB),a back-arc sedimentary basin in a continent-ocean tran...The study of back-arc sedimentary basins is key to understanding oceanic and continental geodynamic processes and mechanisms.The East China Sea Shelf Basin(ECSSB),a back-arc sedimentary basin in a continent-ocean transition zone of the western Pacific Ocean,exhibits remarkable differences in terms of the tectonic deformation characteristics of the basin and the evolutionary features of its sags.These features have led to varied understandings of deep dynamic mechanisms driving the evolution of the basin.In this study,which is based on a detailed interpretation of two-dimensional multichannel seismic data from the ECSSB,the tectonic deformation of the basin is analyzed and the tectonic evolutionary process of the basin is reconstructed.The above research work combines plate convergence processes and deep crust-mantle interactions to explore the controlling factors and deep dynamic mechanisms of the tectonic deformation in the ECSSB.The results of this study indicate that there are significant differences in tectonic deformation between the southern and northern parts of the basin and that the deformation is characterized by eastward migration.Deep crust-mantle interactions are coupled with the development of these differences.The tectonic deformation of the ECSSB is driven by the interactions of the paleo-Pacific,Pacific,Indian,and Philippine Sea Plates with the Eurasian Plate,which have shaped the tectonic pattern of the ECSSB,resulting in east-west zoning,north-south blocking,and pronounced spatiotemporal variability in tectonic deformation.This research provides new insights into the differential development of sags within the ECSSB and provides an important reference for studying back-arc sedimentary basins.展开更多
基金Supported by the Regional Science and Technology Project of Fujian Province,China(2009N3007)Science and Technology Project of Quanzhou City,Fujian Province,China(2008ZD11-1)12396 Multimedia Information Remote Service Platform Construction of Spark Science and Technology for Quanzhou City,Fujian Province,China(2008ZD15,2011ZD04N01)
文摘According to practice and exploration of spark program for 26 years in Quanzhou,the main model and their effects of new rural construction in west bank of Taiwan Strait led by spark science and technology were expounded. Six spark program systems were established,consisting of policy support guide,science and technology project lead,experts' intelligence support,spark science and technology training,scitech information service and spark program demonstration. Five spark projects were implemented to promote new rural construction in the west bank of Taiwan Strait,such as constructing the national spark industrial zone,organizing the new rural construction led by science and technology,constituting spark sci-tech innovation center,developing the rural informatization,and establishing science and technology commissioner bases. Finally,enlightenment of spark program and its development ways in the future were put forward.
文摘InP quantum dots(QDs)have been a major building block of modern display technology due to their high photoluminescence quantum yield(PLQY)in the visible spectrum,superior stability,and eco-friendly composition.However,their applications at short-wave infrared(SWIR)have been hindered by their low efficiency.Here,we report the synthesis of efficient and SWIR-emitting InP QDs by precisely controlling the InP core nucleation using a low-cost ammonia phosphorus precursor,while avoiding size-limiting ZnCl_(2) for effective copper doping.Subsequent epitaxial growth of a lattice-matched ZnSe/ZnS multishell enhanced the QD sphericity and surface smoothness and yielded a record PLQY of 66% with an emission peak at 960 nm.When QDs were integrated as the high-refractive-index luminescent core of a liquid waveguide-based luminescent solar concentrator(LSC),the device achieved an optical efficiency of 7.36%.This performance arises from their high PLQY,spectral alignment with the responsivity peak of silicon solar cells,and the optimized core/cladding waveguide structure.These results highlight the potential of InP QDs as a promising nanomaterial for SWIR emission and applications.
基金supported by the Northeast Geological Science and Technology Innovation Center of China Geological Survey(Grant NO.QCJJ2022-43)the Natural Resources Comprehensive Survey Project(Grant Nos.DD20230470,DD20230508)the National Groundwater Monitoring Network Operation and Maintenance Program(Grant No.DD20251300109).
文摘Excessive levels of Fluoride(F−)and Cadmium(Cd)in drinking groundwater may pose health risks.This study assessed the health risks associated with F−and Cd contamination in rural drinking groundwater sources in Wutai County,Shanxi Province,China,to support population health protection,water resource management,and environmental decision-making.Groundwater samples were collected and analyzed,and a Human Health Risk Model(HHRA)was applied to evaluate groundwater quality.The results showed that both contents of F−and Cd in groundwater exceeded the Class III limits of China's national groundwater quality standard(GB/T 14848—2024).Fluoride levels met the Class V threshold,with enrichment area mainly located in the east part of the study area.Cadmium levels reached Class IV,with elevated concentrations primarily observed in the western and northwestern regions.Correlation analysis revealed that F−showed weak or no correlation with other measured substances,indicating independent sources.Health risk assessment results indicated that F−poses potential health risks to rural residents,while cadmium,due to its relatively low concentrations,does not currently present a significant health risk.Among different demographic groups,the health risk levels of F−exposure followed the order:Infants>children>adult females>adult males.The findings highlight that fluoride is the primary contributor to health risks associated with groundwater consumption in the study area.Strengthened monitoring and prevention of F−contamination are urgently needed.This research provides a scientific basis for the prevention and control of fluoride pollution in groundwater and offers practical guidance for safeguarding drinking water safety in rural China.
基金supported by the Guangdong Province Science and Technology Project"Guangdong Province Industry-University-Research Collaborative Innovation Model"(2015A080804003)。
文摘President Xi,focusing on building a new pattern of all-round opening up to the outside world and promoting the common prosperity and progress of all countries,put forward a major proposal for the construction of the Silk Road Economic Belt and the maritime Silk Road in twenty-first Century.The bay area economy as an important coastal economic form,is the highlight of the current international economic map,and is a significant symbol of the world's first-class coastal city.The international first-class bay areas,such as New York Bay area,San Francisco Bay area and Tokyo Bay area,are characterized by openness,innovation,livability and internationalization.The Guangdong-Hong Kong-Macao Greater Bay Area has four worldclass cities,name Guangzhou,Shenzhen,Hong Kong and Macao,and the Pearl River Delta city cluster.It has an open economic structure,efficient resource allocation ability,strong agglomeration and spillover function and development international communication network plays a core function of leading innovation and gathering radiation.It is an important growth pole for promoting the economic development of the maritime Silk Road and a leader in technological change.With the help of Portugal's geographical position on the maritime Silk Road and market access advantages within the EU system,the Greater Bay Area should further deepen scientific and technological cooperation,improve the ability of scientific and technological innovation of both sides,let the scientific research subjects of the bay area help Portugal's scientific and technological development,and enhance PortugaFs scientific and technological position in the EU.From the perspective of the Belt and Road Initiative,combined with the basic situation and cooperation of China,especially Guangdong,Hong Kong and Macao,and the development of science and technology in Portugal,this article explores the technological fields,policy measures that are suitable for scientific and technological cooperation between the two sides,and puts forward corresponding suggestions to contribute to the technological development of China,Portugal and the global economic and social sustainable development.
基金Supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2012BAD35B04)
文摘Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.
基金This work was supported by the grants from the Special Project of Ministry of Health (No.201302009)and the National Natural Science Foundation of China (No. 81700300).
文摘Primary hyperoxaluria type 1(PH1)is a rare but devastating autosomal recessive inherited disease caused by mutations in gene AGXT.Pathogenic mutations of AGXT were mostly reported in Caucasian but infrequently in Asian,especially in Chinese.To update the genotypes of PH1 in the Chinese population,we collected and identified 7 Chinese probands with PH1 from 2013 to 2017 in our center,five of whom had delayed diagnosis and failed in kidney transplantation.Samples of peripheral blood DNA from the 7 patients and their family members were collected and sequencing analysis was performed to test the mutations of gene AGXT.Western blotting and enzyme activity analysis were conducted to evaluate the function of the mutations.Furthermore,a systematic review from 1998 to 2017 was performed to observe the genetic characteristics between Chinese and Caucasian. The results showed that a total of 12 mutations were identified in the 7 pedigrees.To the best of ourknowledge,2 novel variants of A GXT,p.Gly41 Trp and p.Leu33Met,were first reported.Bioinformatics and functional analysis showed that only 7 mutations led to a reduced expression of alanine-glyoxylate amino transferase (AGT)at a protein level.The systematic review revealed significant population heterogeneity in PH1.In conclusion,new genetic subtypes and genetic characteristics of PH1 are updated in the Chinese population. Furthermore,a genotype-phenotype correlation is found in PH1.
基金supported by the National Natural Science Foundation of China, No.82274616the Key Laboratory Project for General Universities in Guangdong Province, No.2019KSYS005Guangdong Province Science and Technology Plan International Cooperation Project, No.2020A0505100052 (all to QW)。
文摘Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.
基金supported by the National Key Research and Development Program(No.2021YFD1300201)Jilin Provincial Department of Science and Technology Innovation Platform and Talent Special Project(No.20230508090RC).
文摘Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization,energy metabolism,and gut microbiota in growing pigs.Methods Thirty-six growing barrows(47.2±1.5 kg)were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3β-glucan-to-arabinoxylan ratios.In the experiment,nutrient utilization,energy metabolism,fecal microbial community,and production and absorption of short-chain fatty acid(SCFA)of pigs were investigated.In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut.Results The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber.In animal experiments,increasing the dietary apparent viscosity and theβ-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility(AID),apparent total tract digestibility(ATTD),and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter(P<0.05).In addition,increasing dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased gas exchange,heat production,and protein oxidation,and decreased energy deposition(P<0.05).The dietary apparent viscosity andβ-glucanto-arabinoxylan ratios had linear interaction effects on the digestible energy,metabolizable energy,retained energy(RE),and net energy(NE)of the diets(P<0.05).At the same time,the increase of dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased SCFA production and absorption(P<0.05).Increasing the dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria(P<0.05)and the relative abundance of beneficial bacteria.Furthermore,increasing the dietaryβ-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta(P<0.001).Finally,the prediction equations for RE and NE were established.Conclusion Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization,energy metabolism,and pig gut microbiota composition and metabolites.
基金supported by the National Natural Science Foundation of China(Grant No.42076214)Natural Science Foundation of Shandong Province(Grant No.ZR2024QD057).
文摘Timely and accurate forecasting of storm surges can effectively prevent typhoon storm surges from causing large economic losses and casualties in coastal areas.At present,numerical model forecasting consumes too many resources and takes too long to compute,while neural network forecasting lacks regional data to train regional forecasting models.In this study,we used the DUAL wind model to build typhoon wind fields,and constructed a typhoon database of 75 processes in the northern South China Sea using the coupled Advanced Circulation-Simulating Waves Nearshore(ADCIRC-SWAN)model.Then,a neural network with a Res-U-Net structure was trained using the typhoon database to forecast the typhoon processes in the validation dataset,and an excellent storm surge forecasting effect was achieved in the Pearl River Estuary region.The storm surge forecasting effect of stronger typhoons was improved by adding a branch structure and transfer learning.
基金the National Natural Science Foundation of China(Nos.42076220,42206234,42476228)the Laoshan Laboratory Science and Technology Innovation Project(Nos.LSKJ202203404,LSKJ202203401)+2 种基金the Laoshan Laboratory‘14th FiveYear Plan’Major Project(No.2021QNLM020001-1)the Project of China Geological Survey(Nos.DD20230317,DD20230410,DD20190818,DD20191032,DD20160152)the Asia Cooperation Foundation‘China-Pakistan Oil and Gas Resource Potential Assessment and Capacity Training’。
文摘Recent advances in earth science and exploration have made deepwater channel-levee systems a research focus.We collected and analyzed over 10000 km of two-dimensional multichannel seismic data from the offshore Indus Basin to identify channellevee systems at various hierarchical levels depending on their seismic reflection characteristics.Seismic facies analysis was integrated with well data to map the spatial distribution of channel-levee systems in the offshore Indus Basin across various geological periods,and the factors influencing their development were discussed.These systems within the basin were identified using a developed,refined three-tier classification method.The first-order system consists of multiple spatially stacked complexes,the second-order system continuously developed multistage channel-levee bodies,and the third-order system represents the smallest identifiable sedimentary units on seismic profiles.Our findings demonstrate the evolution of the offshore Indus Basin from a single-stage channel with lateral migration to multistage vertical channel stacking from the Miocene to the Pleistocene.Tectonic activities exert their effect on channel-levee systems through their influence on the relative sea level.They also trigger volcanic or seismic events and affect siliciclastic supply.Warm and humid climate conditions form large river systems,which aid in the transport of terrestrial debris to the basin margin.Most channel-levee systems are assumed to have formed during low sea-level periods.This study offers new insights into the formation and evolution of turbidite sedimentary systems in the offshore Indus Basin and presents a practical classification method for comprehending gravity-flow sedimentary configurations and deepwater hydrocarbon exploration.
基金supported by NSFC Shiptime Sharing Project(Nos.42349302,and 42149905)the Hubei Provincial Natural Science Foundation of China(No.2024AFB515)+4 种基金the National Natural Science Foundation of China(Nos.42207173,41831280,and 42176071)the National Key R&D Program of China(No.2024YFC3082500)the Shandong Provincial Natural Science Foundation of China(No.ZR2022QD002)the Shandong Provincial Taishan Scholar Construction Project(No.tsqn202507091)the Shandong Provincial Young Innovators Team(No.2024KJH183).
文摘Low–angle submarine landslides pose a greater threat to offshore infrastructure compared to those with steep sliding angles.Understanding the preparation and triggering mechanism of these low–angle submarine landslides remains a significant challenge.This study focuses on a deformed low–angle submarine landslide in the shelf–slope break of the Pearl River Mouth Basin,South China Sea,integrating sedimentology,geophysics,and geotechnology to investigate potential failure mechanisms.The architecture and deformation characteristics of the submarine landslide were elucidated by analyzing multibeam and seismic data.Within the context of the regional geological history and tectonic framework,this study focuses on the factors(e.g.,rapid sedimentation,fluid activity,and earthquakes)that potentially contributed to the submarine slope failure.Furthermore,a series of stability evaluations considering the effects of rapid sedimentation and earthquakes was conducted.Our findings indicate that the most probable triggering mechanism involves the combined effects of sedimentation controlled by sea–level fluctuations,high–pressure gas activity,and seismic events.The high–pressure gas,which acts as a long–term preconditioning factor by elevating pore pressures and reducing shear resistance within the sediment,accumulated beneath the upper and middle sections of the low–permeability stratum that was formed during sea–level rise and ultimately evolved into the sliding mass.The overpressure generated by gas accumulation predisposed the submarine slope to instability,and a frequent or moderate earthquake ultimately initiated local failure.This study enhances the mechanistic understanding of low–angle slope failures in the shelf–slope break zone and provides critical insights for assessing marine hazard risks.
基金The Fund of Laoshan Laboratory under contract No.LSKJ202202700the Basic Scientific Fund for National Public Research Institutes of China under contract No.2024Q02+1 种基金the National Natural Science Foundation of China under contract Nos 42076023 and 42430402the Global Change and Air-Sea InteractionⅡProject under contract No.GASI-01-ATP-STwin.
文摘The Indonesian Throughflow(ITF)plays important roles in global ocean circulation and climate systems.Previous studies suggested the ITF interannual variability is driven by both the El Niño-Southern Oscillation(ENSO)and the Indian Ocean Dipole(IOD)events.The detailed processes of ENSO and/or IOD induced anomalies impacting on the ITF,however,are still not clear.In this study,this issue is investigated through causal relation,statistical,and dynamical analyses based on satellite observation.The results show that the driven mechanisms of ENSO on the ITF include two aspects.Firstly,the ENSO related wind field anomalies driven anomalous cyclonic ocean circulation in the western Pacific,and off equatorial upwelling Rossby waves propagating westward to arrive at the western boundary of the Pacific,both tend to induce negative sea surface height anomalies(SSHA)in the western Pacific,favoring ITF reduction since the develop of the El Niño through the following year.Secondly,the ENSO events modulate equatorial Indian Ocean zonal winds through Walker Circulation,which in turn trigger eastward propagating upwelling Kelvin waves and westward propagating downwelling Rossby waves.The Rossby waves are reflected into downwelling Kelvin waves,which then propagate eastward along the equator and the Sumatra-Java coast in the Indian Ocean.As a result,the wave dynamics tend to generate negative(positive)SSHA in the eastern Indian Ocean,and thus enhance(reduce)the ITF transport with time lag of 0-6 months(9-12 months),respectively.Under the IOD condition,the wave dynamics also tend to enhance the ITF in the positive IOD year,and reduce the ITF in the following year.
基金supported by the National Nature Science Foundation of China(12463009)the Yunnan Fundamental Research Projects(202301AV070007,202401AU070026)+2 种基金the"Yunnan Revitalization Talent Support Program"Innovation Team Project(202405AS350012)the Scientific Research Foundation Project of Yunnan Education Department(2023J0624,2024Y469)the GHfund A(202407016295)。
文摘Solar activity plays an important role in influencing space weather,making it important to understand numerous aspects of spatial and temporal variations in the Sun's radiative output.High-performance deep learning models and long-term observational records of sunspot relative numbers are essential for solar cycle forecasting.Using the multivariate time series of monthly sunspot relative numbers provided by the National Astronomical Observatory of Japan and two Informer-based models,we forecast the amplitude and timing of solar cycles 25 and 26.The main results are as follows:(1)The maximum amplitude of solar cycle 25 is higher than the previous solar cycle 24 and the following solar cycle 26,suggesting that the long-term oscillatory variation of sunspot magnetic fields is related to the roughly centennial Gleissberg cyclicity.(2)Solar cycles 25 and 26 exhibit a pronounced Gnevyshev gap,which might be caused by two non-coincident peaks resulting from solar magnetic flux transported by meridional circulation and mid-latitude diffusion in the convection zone.(3)Hemispheric prediction of sunspot activity reveals a significant northsouth asynchrony,with activity level of the Sun being more intense in the southern hemisphere.These results are consistent with expectations derived from precursor methods and dynamo theories,and further provide evidence for internal changes in solar magnetic field during the decay of the Modern Maximum.
基金supported by the 2024 Talent Project of Shandong First Medical University(045RC200008)the Taishan Scholars Program(tsqn202211225).
文摘Ferroptosis is a form of cell death elicited by an imbalance in intracellular iron concentrations,leading to enhanced lipid peroxidation.In neurological disorders,both oxidative stress and mitochondrial damage can contribute to ferroptosis,resulting in nerve cell dysfunction and death.The ubiquitin-proteasome system(UPS)refers to a cellular pathway in which specific proteins are tagged with ubiquitin for recognition and degradation by the proteasome.In neuro-logical conditions,the UPS plays a significant role in regu-lating ferroptosis.In this review,we outline how the UPS regulates iron metabolism,ferroptosis,and their interplay in neurological diseases.In addition,we discuss the future application of small-molecule inhibitors and identify poten-tial drug targets.Further investigation into the mechanisms of UPS-mediated ferroptosis will provide novel insights and strategies for therapeutic interventions and clinical applica-tions in neurological diseases.
基金supported by grants from the National Natural Science Foundation of China(22076104)the“Taishan Scholars”Program for Young Expert of Shandong Province(tsqn202103105).
文摘Ferroptosis,a type of programmed cell death,represents a distinct paradigm in cell biology.It is characterized by the iron-dependent accumulation of reactive oxygen species,which induce lipid peroxidation(LPO),and is orchestrated by the interplay between iron,lipid peroxides,and glutathione.In this review,we emphasize the frequently overlooked role of iron in LPO beyond the classical iron-driven Fenton reaction in several crucial processes that regulate cellular iron homeostasis,including iron intake and export as well as ferritinophagy,and the emerging roles of endoplasmic reticulum-resident flavoprotein oxidoreductases,especially P450 oxidoreductases,in modulating LPO.We summarize how various types of fatty acids(FAs),including saturated,monounsaturated,and polyunsaturated FAs,differentially influence ferroptosis when incorporated into phospholipids.Furthermore,we highlight the therapeutic potential of targeting LPO to mitigate ferroptosis and discuss the regulatory mechanisms of endogenous lipophilic radical-trapping antioxidants that confer resistance to ferroptosis,shedding light on therapeutic avenues for ferroptosis-associated diseases.
文摘Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating excitationcontraction(E-C)coupling.Mutations in JPH2 have been associated with hypertrophic cardiomyopathy(HCM),but the molecular mechanisms governing its membrane-binding properties and the functional relevance of its membrane occupation and recognition nexus(MORN)repeat motifs remain incompletely understood.This study aimed to elucidate the structural basis of JPH2 membrane association and its implications for HCM pathogenesis.Methods A recombinant N-terminal fragment of mouse JPH2(residues 1-440),encompassing the MORN repeats and an adjacent helical region,was purified under near-physiological buffer conditions.X-ray crystallography was employed to determine the structure of the JPH2 MORN-Helix domain.Sequence conservation analysis across species and junctophilin isoforms was performed to assess the evolutionary conservation of key structural features.Functional membrane-binding assays were conducted using liposome co-sedimentation and cell-based localization studies in COS7 and HeLa cells.In addition,site-directed mutagenesis targeting positively charged residues and known HCM-associated mutations,including R347C,was used to evaluate their effects on membrane interaction and subcellular localization.Results The crystal structure of the mouse JPH2 MORN-Helix domain was resolved at 2.6Å,revealing a compact,elongated architecture consisting of multiple tandem MORN motifs arranged in a curved configuration,forming a continuous hydrophobic core stabilized by alternating aromatic residues.A C-terminalα-helix further reinforced structural integrity.Conservation analysis identified the inner groove of the MORN array as a highly conserved surface,suggesting its role as a protein-binding interface.A flexible linker segment enriched in positively charged residues,located adjacent to the MORN motifs,was found to mediate direct electrostatic interactions with negatively charged phospholipid membranes.Functional assays demonstrated that mutation of these basic residues impaired membrane association,while the HCM-linked R347C mutation completely abolished membrane localization in cellular assays,despite preserving the overall MORN-Helix fold in structural modeling.Conclusion This study provides structural insight into the membrane-binding mechanism of the cardiomyocyte-specific protein JPH2,highlighting the dual roles of its MORN-Helix domain in membrane anchoring and protein interactions.The findings clarify the structural basis for membrane targeting via a positively charged linker and demonstrate that disruption of this interaction—such as that caused by the R347C mutation—likely contributes to HCM pathogenesis.These results not only enhance current understanding of JPH2 function in cardiac E-C coupling but also offer a structural framework for future investigations into the assembly and regulation of JMCs in both physiological and disease contexts.
基金supported by the National Natural Science Foundation of China(32325050)Natural Science Foundation of Zhejiang Province(LQN25C190013)+2 种基金State Key Program of Natural Science Foundation of Ningbo(2023J005)General Projects of Zhejiang Provincial Department of Education(Y202456633)K.C.Wong Magna Fund in Ningbo University。
文摘Apoptosis preserves organismal homeostasis by selectively eliminating unnecessary or damaged cells, with accumulating evidence also suggesting that it activates regenerative pathways and facilitates tissue remodeling. To date, however, the regulatory mechanisms linking this form of programmed cell death to regeneration remain poorly defined, particularly in evolutionarily basal organisms. Using the sea cucumber (Apostichopus japonicus) as a model for intestinal regeneration, this study identified robust apoptotic activity across key regenerative stages. Pharmacological suppression of apoptosis during wound healing and mesenteric scaffold formation critically impaired intestinal regeneration. Quantitative proteomics using direct data-independent acquisition (DIA) revealed coordinated down-regulation of lipid metabolic pathways under apoptosis-inhibited conditions, with notable suppression of Ca^(2+)-independent phospholipase A2 (iPLA2), an enzyme typically up-regulated during successful regeneration. In parallel, expression of regeneration-associated factors WNT6 and EGFL7 was markedly reduced under apoptotic blockade. Targeted inhibition of iPLA2, EGFL7, or WNT6 each resulted in impaired mesenteric outgrowth and reduced proliferative activity within the regenerating intestinal primordia. Collectively, these findings suggest two potential mechanistic pathways: apoptosis-mediated regeneration of lipid metabolism via iPLA2 and apoptosis-dependent activation of WNT6/EGFL7 signaling. This study provides mechanistic insight into apoptosis-coupled regenerative processes in basal deuterostomes and expands the conceptual framework of programmed cell death in tissue renewal.
基金the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.LSKJ202203206)the Fundamental Research Funds for the Central Universities(No.202161069)。
文摘Blooms of the toxic dinoflagellate Alexandrium spp.,which produce paralytic shellfish toxins(PSTs),have threatened marine ecosystems for decades.However,their impacts on benthic copepods remain underexplored.Here,we investigated the physiological and biochemical responses of marine benthic copepod Tigriopus japonicus to Alexandrium pacificum.Adult female showed no mortality within 24 h and maintained high survival(>80%)across all A.pacificum concentrations over 96 h,with no significant difference from non-toxic controls.Ingestion rates on A.pacificum and non-toxic controls were similar,when algal prey concentrations were lower than about 2500µgC/L.However,above this threshold,feeding of T.japonicus was significantly depressed by A.pacificum.Six-day exposure induced oxidative stress,marked by elevated level of reactive oxygen species,malondialdehyde,total antioxidant capacity and superoxide dismutase activity,while catalase activity showed a non-significant increase.Moreover,concentration-dependent increase was observed in acetylcholinesterase activity,and its positive correlation with oxidative biomarkers suggests additional roles in coping with stress.Chronic exposure over three generations did not consistently affect development,except for complete development failure in F2 and F3 at the highest concentration.However,moderate-to-high concentrations of A.pacificum significantly impaired reproduction,reducing egg production by 9.66%–96.07%and clutch numbers by 33.33%–55.56%.Our findings indicate that Alexandrium blooms can adversely impact benthic copepod feeding and population recruitment through neurotoxicity and oxidative stress,highlighting the ecological risks of prolonged algal blooms in benthic habitats.
基金grants from the National Key R&D Program of China(No.2022YFD2400305)the Excellent Seed Project of Shandong Province(No.2022LZGCQY010)+1 种基金the Science and Technology Program of Fujian Province(No.2024N3003)the Research on Breeding Technology of Candidate Species for Guangdong Modern Marine Ranching(No.2024-MRB00-001)。
文摘Recently,allotriploids from Pacific oysters(Crassostrea gigas)and Fujian oysters(C.angulata)have been grown for aquaculture.However,the viability of these allotriploids remains uncertain.In this study,two autotriploids,TGG(diploid C.gigas♀×tetraploid C.gigas♂)and TAA(diploid C.angulata♀×tetraploid C.angulata♂),and two allotriploids,TGA(diploid C.gigas♀×tetraploid C.angulata♂)and TAG(diploid C.angulata♀×tetraploid C.gigas♂),were studied to assess the heterosis in growth and survival at different temperatures and salinities.The results showed that during the larval stage,TAG exhibited increased heterosis in growth and survival when temperature rose.During the adult stage,the growth of TGA significantly outperformed other triploids at higher temperatures(23 and 28℃)and salinities(25 and 30).In contrast,TAG demonstrated the highest survival probability across all conditions except at salinity 25,where it equaled TAA after day 19.The highest oxygen consumption rates(OCR)of TGA and TAG were observed at 23 and 28℃,while the ammonia excretion rate(AER)of TAG was significantly higher than that of TGA at 23℃.These physiological parameters reflect the advantage of TAG in terms of survival.Notably,the temperature coefficient of allotriploids was higher than that of autotriploids in the range of 18–23℃.At 28℃,TAG showed the highest superoxide dismutase(SOD)and catalase(CAT)activities and the lowest malondialdehyde(MDA)content,showing its advantage when encountering high-temperature.
基金the Laoshan Laboratory(No.LSKJ202203401)the Geological Survey Projects of the China Geological Survey(Nos.DD20221723,DD20243116)+2 种基金the Natural Science Foundation of Shandong Province(No.ZR2023MD047)the Major Basic Research Project of Shandong Province(No.ZR2021ZD09)the National Natural Science Foundation of China(No.42476077)。
文摘The study of back-arc sedimentary basins is key to understanding oceanic and continental geodynamic processes and mechanisms.The East China Sea Shelf Basin(ECSSB),a back-arc sedimentary basin in a continent-ocean transition zone of the western Pacific Ocean,exhibits remarkable differences in terms of the tectonic deformation characteristics of the basin and the evolutionary features of its sags.These features have led to varied understandings of deep dynamic mechanisms driving the evolution of the basin.In this study,which is based on a detailed interpretation of two-dimensional multichannel seismic data from the ECSSB,the tectonic deformation of the basin is analyzed and the tectonic evolutionary process of the basin is reconstructed.The above research work combines plate convergence processes and deep crust-mantle interactions to explore the controlling factors and deep dynamic mechanisms of the tectonic deformation in the ECSSB.The results of this study indicate that there are significant differences in tectonic deformation between the southern and northern parts of the basin and that the deformation is characterized by eastward migration.Deep crust-mantle interactions are coupled with the development of these differences.The tectonic deformation of the ECSSB is driven by the interactions of the paleo-Pacific,Pacific,Indian,and Philippine Sea Plates with the Eurasian Plate,which have shaped the tectonic pattern of the ECSSB,resulting in east-west zoning,north-south blocking,and pronounced spatiotemporal variability in tectonic deformation.This research provides new insights into the differential development of sags within the ECSSB and provides an important reference for studying back-arc sedimentary basins.
