Objective To evaluate whether the clustering of risk factors, both environmental and genetic, increases the risk of essential hypertension （EH） and the accumulation of risk factors influences the blood pressure leve...Objective To evaluate whether the clustering of risk factors, both environmental and genetic, increases the risk of essential hypertension （EH） and the accumulation of risk factors influences the blood pressure level in normotensives. Methods On the basis of a prevalence survey, 501 subjects of Mongolian ethnicity （243 hypertensives and 258 normotensives） who were not related to each other were selected to conduct a case-control study. All subjects were interviewed with questionnaires and their blood specimens were collected. Renin gene insertion/deletion （I/D） polymorphism, a new genetic marker, was genotyped with PCR and polyacrylamide gel electrophoresis. Results Overweight, alcohol consumption, and renin gene I/D polymorphism were significant risk factors of EH （P〈0.05）. The odds ratios （OR） for the number of risk factors were 2.39 （95%CI： 0.98-6.74） for one risk factor, 5.03 （95%CI： 2.06-14.18） for two, and 6.09 （95%CI： 1.85-22.38） for three respectively after adjusting for age and sex. In normotensives, age- and sex-adjusted mean blood pressures increased with more accumulation of risk factors. However, there were no significant differences among the different blood pressure levels according to the number of risk factors （P〉0.05）. Conclusion Overweight, alcohol consumption, and renin gene I/D polymorphism are risk factors of EH in the Mongolian ethnic population of China. The accumulation of the risk factors causes a sharp increase of the risk of EH.展开更多
After the publication of this work,1 the authors noticed and confirmed that the Funding Information was mistakenly omitted from the article.The statement“This study was supported by grants from the National Natural S...After the publication of this work,1 the authors noticed and confirmed that the Funding Information was mistakenly omitted from the article.The statement“This study was supported by grants from the National Natural Science Foundation of China,grant no.81370041,81471760,81671655,the Outstanding Clinical Discipline Project of Shanghai Pudong,grant no.PWYgy2018-04.The authors declare that they have no conflicts of interest.”should be included in the Funding information section of the paper which is missing.We apologize for the error.展开更多
Introduction In recent years,three-dimensional printing(3DP),an additive manufacturing process,has gained widespread clinical application,and 3DP has been considered as the third industrial revolution.1 In its early i...Introduction In recent years,three-dimensional printing(3DP),an additive manufacturing process,has gained widespread clinical application,and 3DP has been considered as the third industrial revolution.1 In its early introduction in the 1980s,3DP served as a software-controlled technology that converted computer-aided-design(CAD)data into a physical object via a single process.By depositing multiple two-dimensional cross-sections one above the other,3DP can now be used to build arbitrarily complex geometries and patient-specific constructs using the patient’s imaging data.Till date,computed tomography has been the main imaging data source for 3DP owing to its excellent spatial resolution.Furthermore,current 3D printers have enabled bedside on-demand fabrication of medical products in hospitals.New materials including polymers,ceramics,biomaterials,and metals have been developed for such applications over the last few decades.Medical fields that employ 3DP technologies have also expanded,such as tissue engineering,regenerative medicine,pharmaceutics,and medical models and devices.2 The market for additive manufacturing is expected to surpass$20 billion in the global industry by the end of the 2020.3 Although the use of 3DP technology in interventional medicine is still relatively new,advancements are occurring within this discipline at a rapid rate.Different 3DP technologies,materials,and clinical applications relevant to the interventional field are discussed in this article.展开更多
Right-sided aortic arch with aberrant left subclavian artery (RAA/ALSC) is the second most common mediastinal complete vascular ring. Adult presentation of dysphagia lusoria due to a RAA/ALSC is uncommon with fewer th...Right-sided aortic arch with aberrant left subclavian artery (RAA/ALSC) is the second most common mediastinal complete vascular ring. Adult presentation of dysphagia lusoria due to a RAA/ALSC is uncommon with fewer than 25 cases reported in the world literature. The left lateral portion of this vascular ring is not a vessel, but an atretic ductus arteriosus, the ligamentum arteriosum, which has been identified in different cases as the major cause of tracheo-esophageal impingement. Surgical division of the ligamentum arteriosum allows the vessels to assume a less constricting pattern decreasing dysphagic symptoms. Clear visualization of the ligamentum arteriosum by diagnostic imaging has not been obtained in previously reported cases. We demonstrated, using magnetic resonance imaging, the location and the complete course of a left-sided ligamentum arteriosum in a patient with adult-onset dysphagia due to a RAA/ALSC with a small Kommerell' s diverticulum, providing, during the same session, a complete assessment of both mediastinal vascular abnormalities and esophageal impingement sites.展开更多
An anharmonic oscillator algebra model is used to study the collinear collisions of two diatomic molecules. The transition probability for vibration-vibration energy transfer is presented. For an application of the me...An anharmonic oscillator algebra model is used to study the collinear collisions of two diatomic molecules. The transition probability for vibration-vibration energy transfer is presented. For an application of the method, we talk about the collision of N2＋CO, N2＋O2, and N2＋N2. Through long time averaging, the transition probability changes to the function of total energy of the system. Comparing the results with the quantum results, we can see that the dynamical Lie algebraic method is useful for describing the anharmonie diatomic molecular collision.展开更多
The lead glass used in an X-ray examination room is indispensable for watching a subject being tested or a situation of operation of an X-ray generator, but because it is some structure equipment of an X-ray examinati...The lead glass used in an X-ray examination room is indispensable for watching a subject being tested or a situation of operation of an X-ray generator, but because it is some structure equipment of an X-ray examination room, it must obey legal restrictions. Although the standard for lead glass is determined by the International Electrotechnical Commission (IEC), there are few reports about the characteristics related to interaction with radiation. We performed a comparison of three lead glass products, which are principally used in world. As for three products, differences are not looked by physics and chemical characteristics features. Simply the difference was looked by the coloring characteristic for an interaction with radiation. Furthermore, we checked that temporal decolorization of coloring had two half-lives. The short half-life is about 28 hours and the long half-life is about 107 days.展开更多
Background:Brain metastases are frequent complications for lung cancer patients.However,changes in the brain of lung cancer patients have received little attention.We aimed to explore whether alterations in brain gluc...Background:Brain metastases are frequent complications for lung cancer patients.However,changes in the brain of lung cancer patients have received little attention.We aimed to explore whether alterations in brain glucose uptake and brain texture occur in non-small cell lung cancer(NSCLC)patients and to investigate associations between brain alterations and NSCLC via the uEXPLORER positron emission tomography/computed tomography(PET/CT)system.Methods:In total,105 participants were enrolled,including 55 healthy con-trols and 50 NSCLC patients.Images were acquired using the PET/CT system.Standardized uptake values normalized by lean body mass were calculated as indicators of glucose uptake.Correlation analysis was conducted between aberrant brain glucose uptake,glucose uptake of cancer lesions,and con-centrations of serum lung cancer markers.Radiomics was used to investigate whether features extracted from regions with altered brain glucose uptake could serve as biomarkers of lung cancer progression.Results:Compared with healthy controls,NSCLC patients showed decreased standardized uptake values normalized by lean body mass in the left insula,medial frontal gyrus,and anterior cingulate.Correlation analysis demon-strated that glucose uptake of the anterior cingulate was negatively correlated with serum lung cancer marker concentrations.Radiomic features on PET/CT images of the above brain regions could classify NSCLC patients and healthy controls with an accuracy of 79%.Conclusions:NSCLC patients exhibited altered brain glucose uptake and changes in brain textures.These alterations may reflect alterations in behav-ioral domains in NSCLC and may be related to altered lung-brain interactions and potential brain metastasis of NSCLC.展开更多
Constructing high-performance nanozymes for specific biomolecules is crucial but challenging for practical applications and fundamental research.Herein,through the examination of the catalytic reaction paths of natura...Constructing high-performance nanozymes for specific biomolecules is crucial but challenging for practical applications and fundamental research.Herein,through the examination of the catalytic reaction paths of natural nicotinamide adenine dinucleotide(NADH)oxidase(NOX),a novel and efficient single-atom rhodium catalyst(Rh1/NC)was developed to mimic NOX.The Rh_(1)/NC demonstrated the ability to catalyze the dehydrogenation of NADH and transfer electrons to O_(2)to generate H_(2)O_(2)through the typical two-electron pathway.Furthermore,our findings revealed that Rh_(1)/NC exhibits the ability to catalyze the conversion of produced H_(2)O_(2)into OH under mildly acidic conditions.This process amplifies the oxidation of NADH,showcasing NADH peroxidase-like activity(NPx-like).As a paradigm,this unique dual enzyme-like property of Rh_(1)/NC with a positive feedback effect holds significance in disrupting cancer cellular homeostasis.Rh_(1)/NC can effectively consume NADH via cascade biocatalytic reactions within cancer cells,further triggering the elevation of reactive oxygen species(ROS),leading to impaired oxidative phosphorylation and decreased mitochondrial membrane potential,thus damaging the adenosine triphosphate(ATP)synthesis.The resulting'domino effect'interferes with the energy metabolism homeostasis of cancer cells,ultimately promoting cell apoptosis.This study provides potential guidance for the rational design of materials with greater capabilities.展开更多
Polydimethylsiloxane(PDMS)is considered a low surface energy material widely used in(super)hydrophobic modification.In this paper,the high hydrophobic melamine sponges(MS)were modified with commercial aminopropyl func...Polydimethylsiloxane(PDMS)is considered a low surface energy material widely used in(super)hydrophobic modification.In this paper,the high hydrophobic melamine sponges(MS)were modified with commercial aminopropyl functionalized polydimethylsiloxane(NH_(2)-PDMS)with different molecular mass.The chemical composition,surface morphology,and wettability of the NH_(2)-PDMS-modified MS were investigated by X-ray photoelectron spectroscopy(XPS),attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR)and contact angle test.Owing to the porous structure and high hydrophobicity,NH_(2)-PDMS-modified MS possesses remarkable absorption capacity(ranging from 46 to 155 times their own mass).Simultaneously,it can effectively separate oil-water mixtures with high separation efficiencies exceeding 98.2%.NH_(2)-PDMS-modified MS has no obvious change after 10 cycles of oil-water separation.The results demonstrate PDMS molecular mass on surface can revise material properties and achieve high separation efficiencies in oil-water separation.展开更多
AIM： To investigate the association between the CpG island methylator phenotype （CIMP） and serum Helico- bacter pylori （H. pylori） levels for clinical prediction of gastric cancer （GC） progression. METHODS： We...AIM： To investigate the association between the CpG island methylator phenotype （CIMP） and serum Helico- bacter pylori （H. pylori） levels for clinical prediction of gastric cancer （GC） progression. METHODS： We analyzed the serum ClMP status of 75 patients with GC using a methylation marker panel and a methylation-specific polymerase chain reaction. Serum samples from 40 healthy persons were examined at the same time. The genes examined were APC, WIF-1, RUNX-3, DLC-1, SFRP-1, DKK and E-cad. H. pylori infec- tion in serum was assayed with an anti-H, pylori immu- noglobulin G antibody test and a rapid urease test. RESULTS： The frequencies of high-level methylation in GC tissues for the seven genes were： 48% for APC, 57.33% for WIF-1, 56% for RUNX-3, 50.67% for DLC-1, 52% for SFRP-1, 54.67% for DKK, and 48% for E-cad.The frequencies in GC serum were 30.67% for APC, 34.67% for WIF-1, 37.33% for RUNX-3, 29.33% for DLC-1, 33.33% for SFRP-1, 32% for DKK, and 26.67% for E-cad. CIMP＋ （defined as ≥ 3 methylated genes） was associated with 47 （62.67%） GC tissue samples and 44 （58.67%） GC serum samples. CIMP＋ was not associated with non-neoplastic mucosal tissues or the serum of healthy persons. Of the 75 GC cases, 51 （68%） were H. pylori＋, and 24 （32%） were H. pylori-. Of the 51 H. pylori＋ cases, 36 were CIMP＋ and 15 were CIMP-. In contrast, for the 24 H. pylori- cases, 11 were CIMP＋, and 13 were CIMP-. The difference was signifi- cant between the H. pylori＋ and H. pylori- groups χ2 = 4.27, P 〈 0.05）. Of the 51 H. pylori＋ GC patients, 34 were CIMP＋ and 17 were CIMP-, while among the 24 H. pylori- GC cases, 10 were CIMP＋ and 14 were CIMP-. The difference was significant between the H. pylori＋ and H. pylori- groups （χ2 = 4.21, P 〈 0.05）. A 2-year follow-up showed significant difference in the rates of metastasis and recurrence between H. pylori＋/CIMP＋ cases and the H. pylori＋/CIMP- cases or CIMP- cases associated with H. pylori assayed in serum （P 〈 0.05）. However, there were no significant differences in sur- vival rates between the two groups. CONCLUSION： H. pylori＋/CIMP＋ cases are associ- ated with higher rates of metastasis and recurrence thanH, pylori＋/CIMP- cases. Serum may be useful for examining CIMP status.展开更多
The earliest and most accurate detection of the pathological manifestations of hepatic diseases ensures effective treatments and thus positive prognostic outcomes.In clinical settings,screening and determining the ext...The earliest and most accurate detection of the pathological manifestations of hepatic diseases ensures effective treatments and thus positive prognostic outcomes.In clinical settings,screening and determining the extent of a pathology are prominent factors in preparing remedial agents and administering approp-riate therapeutic procedures.Moreover,in a patient undergoing liver resection,a realistic preoperative simulation of the subject-specific anatomy and physiology also plays a vital part in conducting initial assessments,making surgical decisions during the procedure,and anticipating postoperative results.Conventionally,various medical imaging modalities,e.g.,computed tomography,magnetic resonance imaging,and positron emission tomography,have been employed to assist in these tasks.In fact,several standardized procedures,such as lesion detection and liver segmentation,are also incorporated into prominent commercial software packages.Thus far,most integrated software as a medical device typically involves tedious interactions from the physician,such as manual delineation and empirical adjustments,as per a given patient.With the rapid progress in digital health approaches,especially medical image analysis,a wide range of computer algorithms have been proposed to facilitate those procedures.They include pattern recognition of a liver,its periphery,and lesion,as well as pre-and postoperative simulations.Prior to clinical adoption,however,software must conform to regulatory requirements set by the governing agency,for instance,valid clinical association and analytical and clinical validation.Therefore,this paper provides a detailed account and discussion of the state-of-the-art methods for liver image analyses,visualization,and simulation in the literature.Emphasis is placed upon their concepts,algorithmic classifications,merits,limitations,clinical considerations,and future research trends.展开更多
Senescence,a multifaceted cellular process,intricately regulates organismal aging by imposing irreversible growth arrest on cells.This phenomenon,characterized by altered gene expression and the accumulation of senesc...Senescence,a multifaceted cellular process,intricately regulates organismal aging by imposing irreversible growth arrest on cells.This phenomenon,characterized by altered gene expression and the accumulation of senescent cells,significantly contributes to age-related physiological decline and the onset of various age-associated pathologies[1].Cellular senescence,the irreversible cessation of cell division,is intricately linked to the aging process in individuals[2].As organisms age,the accumulation of senescent cells increases,contributing to tissue dysfunction and the development of age-related pathologies.Understanding the mechanisms underlying cellular senescence holds promise for elucidating the fundamental processes governing aging and may pave the way for targeted interventions to mitigate age-associated decline[3].展开更多
Benefiting from the unique advantages of superior biocompatibility,strong stability,good biodegradability,and adjustable mechanical properties,hydrogels have attracted extensive research interests in bioelectronics.Ho...Benefiting from the unique advantages of superior biocompatibility,strong stability,good biodegradability,and adjustable mechanical properties,hydrogels have attracted extensive research interests in bioelectronics.However,due to the existence of an interface between hydrogels and human tissues,the transmission of electrical signals from the human tissues to the hydrogel electronic devices will be hindered.The adhesive hydrogels with adhesive properties can tightly combine with the human tissue,which can enhance the contact between the electronic devices and human tissues and reduce the contact resistance,thereby improving the performance of hydrogel electronic devices.In this review,we will discuss in detail the adhesion mechanism of adhesive hydrogels and elaborate on the design principles of adhesive hydrogels.After that,we will introduce some methods of performance evaluation for adhesive hydrogels.Finally,we will provide a perspective on the development of adhesive hydrogel bioelectronics.展开更多
Wound healing is a critical physiological process in living organisms that includes repair cell proliferation and migration,etc.[1,2].While effective wound-healing treatment strategies have been developed in the past,...Wound healing is a critical physiological process in living organisms that includes repair cell proliferation and migration,etc.[1,2].While effective wound-healing treatment strategies have been developed in the past,few have actively controlled the behavior of skin cells to accelerate wound healing[3,4].The importance of accelerated wound healing lies in its ability to shorten treatment time,reduce the risk of infection,and lower the cost of treatment.Electrical stimulation can modulate cell behavior during wound healing by simulating endogenous electric fields,boosting cell migration,fibroblast proliferation,and granulation tissue growth more significantly than traditional methods[5-7].However,electrical stimulation devices’size,space limitations,and unsustainability make them challenging to use in clinical surgery,hindering real-time and convenient treatment.It is therefore crucial to explore the miniaturization of electrical stimulation devices to advance the healing of skin wounds in clinical procedures.Recently,Chen et al.report a flexible micro-Zn-MnO2(mZMB)battery-based woundplast for wound management,which shows a promising path in biomedical applications(Figure 1)[8].展开更多
Osteoarthritis(OA)has emerged as a significant health concern among the elderly population,with increasing attention paid to ferroptosis-induced OA in recent years.However,the prolonged use of nonsteroidal anti-inflam...Osteoarthritis(OA)has emerged as a significant health concern among the elderly population,with increasing attention paid to ferroptosis-induced OA in recent years.However,the prolonged use of nonsteroidal anti-inflammatory drugs or corticosteroids can lead to a series of side effects and limited therapeutic efficacy.This study aimed to employ the Mannich condensation reaction between epigallocatechin-3-gallate(EGCG)and selenomethionine(SeMet)to efficiently synthesize polyphenol-based nanodrugs in aqueous media for treating OA.Molecular biology experiments demonstrated that EGCG-based nanodrugs(ES NDs)could effectively reduce glutathione peroxidase 4(GPX4)inactivation,abnormal Fe2+accumulation,and lipid peroxidation induced by oxidative stress,which ameliorated the metabolic disorder of chondrocytes and other multiple pathological processes triggered by ferroptosis.Moreover,imaging and histopathological analysis of the destabilization of the medial meniscus model in mice confirmed that ES NDs exhibiting significant therapeutic effects in relieving OA.The intra-articular delivery of ES NDs represents a promising approach for treating OA and other joint inflammatory diseases.展开更多
Estrogen receptor (ER), one member of nuclear hormone receptor (NR) family, is an estrogen-dependent transcriptional factor that plays an important role in development, progression and treatment of breast cancer. Tran...Estrogen receptor (ER), one member of nuclear hormone receptor (NR) family, is an estrogen-dependent transcriptional factor that plays an important role in development, progression and treatment of breast cancer. Transcriptional co-factors (co-activators and co-repressors) are critical for ER to transduce hormone and metabolic signaling to target genes. A number of functional and structural studies have elucidated the precise mechanisms of co-activator interaction with the ligand-inducible activation domain in ER via one and several LXXLL motifs (where X is any amino acid) known as NR-Box. By the yeast two-hybrid system we have identified a novel ER-a interacting protein ERIAP (Estrogen Receptor Interacting and Activating Protein) which contains two consensus LXXLL motifs. ERIAP associated with ER-a in a ligand-dependent manner, as demonstrated by in vivo immunoprecipitation and in vitro GST capture assays. The two NR boxes were essential for ERIAP interaction with ER-a. Furthermore, ERIAP specifically enhanced ligand-mediated ER-a transcriptional activity in a dose-dependent fasion and increased the expression of estrogen-responsive gene pS2. Thus, our present findings indicate that ERIAP funcions as a new coactivator for ER-a transcriptional activity, which may play an important role in development and progression of breast cancer.展开更多
Background Attention-deficit/hyperactivity disorder(ADHD)is a common neurodevelopmental disorder with behavioural symptoms and grey matter volume(GMV)changes.However,previous studies have not fully elucidated the prog...Background Attention-deficit/hyperactivity disorder(ADHD)is a common neurodevelopmental disorder with behavioural symptoms and grey matter volume(GMV)changes.However,previous studies have not fully elucidated the progressive and causal GMV changes associated with behavioural symptoms in ADHD.Aims This study aimed to explore the causal relationship between GMV alterations and behavioural symptoms in children and adolescents with ADHD using behaviourcausal structural covariance network(BCaSCN)analysis.Methods Structural magnetic resonance imaging(sMRI)data from 135 children and adolescents with ADHD and182 neurotypical controls(NCs)were analysed.ADHD subtypes were identified based on GMV using a clustering algorithm to address the neuroanatomical heterogeneity.To investigate the causal relationships of GMV changes related to behavioural symptoms,sMRI data were sequentially ordered by ADHD index,inattentive index and hyperactive/impulsive index values to generate pseudotime series data.These data were then analysed using region-of-interest-based BCaSCN analysis to explore potential progressive patterns of GMV change.Results Neuroanatomical subtyping revealed two ADHD subtypes with distinct GMV patterns compared with NCs.BCaSCN analysis showed that ADHD subtype 1 was closely associated with inattentiveness,involving prominent nodes in the frontal regions and cerebellum.In contrast,ADHD subtype 2 was more strongly linked to overall disease severity,with the cerebellum and hippocampus as primary hubs.Conclusions ADHD is associated with heterogeneous changes in GMV corresponding to distinct behavioural domains,highlighting the need for subtype-specific diagnostic and therapeutic strategies.展开更多
Middle infrared stimulation(MIRS)and vibrational strong coupling(VSC)have been separately applied to physically regulate biological systems but scarcely compared with each other,especially at identical vibrational fre...Middle infrared stimulation(MIRS)and vibrational strong coupling(VSC)have been separately applied to physically regulate biological systems but scarcely compared with each other,especially at identical vibrational frequencies,though they both involve resonant mechanism.Taking cell proliferation and migration as typical cell-level models,herein,we comparatively studied the nonthermal bioeffects of MIRS and VSC with selecting the identical frequency(53.5 THz)of the carbonyl vibration.We found that both MIRS and VSC can notably increase the proliferation rate and migration capacity of fibroblasts.Transcriptome sequencing results reflected the differential expression of genes related to the corresponding cellular pathways.This work not only sheds light on the synergistic nonthermal bioeffects from the molecular level to the cell level but also provides new evidence and insights for modifying bioreactions,further applying MIRS and VSC to the future medicine of frequencies.展开更多
基金This study was supported by the National Natural Sciences Foundation of China (Grant No. 30271149)Beijing Municipal NaturalSciences Foundation (No. 7001004)China Medical Board in New York (No. 96-657).
文摘Objective To evaluate whether the clustering of risk factors, both environmental and genetic, increases the risk of essential hypertension （EH） and the accumulation of risk factors influences the blood pressure level in normotensives. Methods On the basis of a prevalence survey, 501 subjects of Mongolian ethnicity （243 hypertensives and 258 normotensives） who were not related to each other were selected to conduct a case-control study. All subjects were interviewed with questionnaires and their blood specimens were collected. Renin gene insertion/deletion （I/D） polymorphism, a new genetic marker, was genotyped with PCR and polyacrylamide gel electrophoresis. Results Overweight, alcohol consumption, and renin gene I/D polymorphism were significant risk factors of EH （P〈0.05）. The odds ratios （OR） for the number of risk factors were 2.39 （95%CI： 0.98-6.74） for one risk factor, 5.03 （95%CI： 2.06-14.18） for two, and 6.09 （95%CI： 1.85-22.38） for three respectively after adjusting for age and sex. In normotensives, age- and sex-adjusted mean blood pressures increased with more accumulation of risk factors. However, there were no significant differences among the different blood pressure levels according to the number of risk factors （P〉0.05）. Conclusion Overweight, alcohol consumption, and renin gene I/D polymorphism are risk factors of EH in the Mongolian ethnic population of China. The accumulation of the risk factors causes a sharp increase of the risk of EH.
文摘After the publication of this work,1 the authors noticed and confirmed that the Funding Information was mistakenly omitted from the article.The statement“This study was supported by grants from the National Natural Science Foundation of China,grant no.81370041,81471760,81671655,the Outstanding Clinical Discipline Project of Shanghai Pudong,grant no.PWYgy2018-04.The authors declare that they have no conflicts of interest.”should be included in the Funding information section of the paper which is missing.We apologize for the error.
文摘Introduction In recent years,three-dimensional printing(3DP),an additive manufacturing process,has gained widespread clinical application,and 3DP has been considered as the third industrial revolution.1 In its early introduction in the 1980s,3DP served as a software-controlled technology that converted computer-aided-design(CAD)data into a physical object via a single process.By depositing multiple two-dimensional cross-sections one above the other,3DP can now be used to build arbitrarily complex geometries and patient-specific constructs using the patient’s imaging data.Till date,computed tomography has been the main imaging data source for 3DP owing to its excellent spatial resolution.Furthermore,current 3D printers have enabled bedside on-demand fabrication of medical products in hospitals.New materials including polymers,ceramics,biomaterials,and metals have been developed for such applications over the last few decades.Medical fields that employ 3DP technologies have also expanded,such as tissue engineering,regenerative medicine,pharmaceutics,and medical models and devices.2 The market for additive manufacturing is expected to surpass$20 billion in the global industry by the end of the 2020.3 Although the use of 3DP technology in interventional medicine is still relatively new,advancements are occurring within this discipline at a rapid rate.Different 3DP technologies,materials,and clinical applications relevant to the interventional field are discussed in this article.
文摘Right-sided aortic arch with aberrant left subclavian artery (RAA/ALSC) is the second most common mediastinal complete vascular ring. Adult presentation of dysphagia lusoria due to a RAA/ALSC is uncommon with fewer than 25 cases reported in the world literature. The left lateral portion of this vascular ring is not a vessel, but an atretic ductus arteriosus, the ligamentum arteriosum, which has been identified in different cases as the major cause of tracheo-esophageal impingement. Surgical division of the ligamentum arteriosum allows the vessels to assume a less constricting pattern decreasing dysphagic symptoms. Clear visualization of the ligamentum arteriosum by diagnostic imaging has not been obtained in previously reported cases. We demonstrated, using magnetic resonance imaging, the location and the complete course of a left-sided ligamentum arteriosum in a patient with adult-onset dysphagia due to a RAA/ALSC with a small Kommerell' s diverticulum, providing, during the same session, a complete assessment of both mediastinal vascular abnormalities and esophageal impingement sites.
基金Supported by the National Science Foundation of China under Grant No. 20173013Partial Financial Supports from the Science Foundation of Shandong Province under Grant No. Y2008C102the Foundation of Taishan Meidical College under Grant No. TSB016
文摘An anharmonic oscillator algebra model is used to study the collinear collisions of two diatomic molecules. The transition probability for vibration-vibration energy transfer is presented. For an application of the method, we talk about the collision of N2＋CO, N2＋O2, and N2＋N2. Through long time averaging, the transition probability changes to the function of total energy of the system. Comparing the results with the quantum results, we can see that the dynamical Lie algebraic method is useful for describing the anharmonie diatomic molecular collision.
文摘The lead glass used in an X-ray examination room is indispensable for watching a subject being tested or a situation of operation of an X-ray generator, but because it is some structure equipment of an X-ray examination room, it must obey legal restrictions. Although the standard for lead glass is determined by the International Electrotechnical Commission (IEC), there are few reports about the characteristics related to interaction with radiation. We performed a comparison of three lead glass products, which are principally used in world. As for three products, differences are not looked by physics and chemical characteristics features. Simply the difference was looked by the coloring characteristic for an interaction with radiation. Furthermore, we checked that temporal decolorization of coloring had two half-lives. The short half-life is about 28 hours and the long half-life is about 107 days.
基金Taishan Scholar Project of Shandong Province,Grant/Award Number:TS201712065Chinese Polar Environment Comprehensive Investigation and Assessment Programmes,Grant/Award Number:2021YFE0204600Science and Technology Development Plan of Shandong Province,Grant/Award。
文摘Background:Brain metastases are frequent complications for lung cancer patients.However,changes in the brain of lung cancer patients have received little attention.We aimed to explore whether alterations in brain glucose uptake and brain texture occur in non-small cell lung cancer(NSCLC)patients and to investigate associations between brain alterations and NSCLC via the uEXPLORER positron emission tomography/computed tomography(PET/CT)system.Methods:In total,105 participants were enrolled,including 55 healthy con-trols and 50 NSCLC patients.Images were acquired using the PET/CT system.Standardized uptake values normalized by lean body mass were calculated as indicators of glucose uptake.Correlation analysis was conducted between aberrant brain glucose uptake,glucose uptake of cancer lesions,and con-centrations of serum lung cancer markers.Radiomics was used to investigate whether features extracted from regions with altered brain glucose uptake could serve as biomarkers of lung cancer progression.Results:Compared with healthy controls,NSCLC patients showed decreased standardized uptake values normalized by lean body mass in the left insula,medial frontal gyrus,and anterior cingulate.Correlation analysis demon-strated that glucose uptake of the anterior cingulate was negatively correlated with serum lung cancer marker concentrations.Radiomic features on PET/CT images of the above brain regions could classify NSCLC patients and healthy controls with an accuracy of 79%.Conclusions:NSCLC patients exhibited altered brain glucose uptake and changes in brain textures.These alterations may reflect alterations in behav-ioral domains in NSCLC and may be related to altered lung-brain interactions and potential brain metastasis of NSCLC.
基金financially supported by the National Natural Science Foundation of China(No.22207066)Taishan Scholars Program of Shandong Province(No.TS201712065)+2 种基金the Academic Promotion Program of Shandong First Medical University(No.2019QL009)the Science and Technology Funding from Jinan(No.2020GXRC018)the Traditional Chinese Medicine Science and Technology Project of Shandong Province(No.Q-2022142)。
文摘Constructing high-performance nanozymes for specific biomolecules is crucial but challenging for practical applications and fundamental research.Herein,through the examination of the catalytic reaction paths of natural nicotinamide adenine dinucleotide(NADH)oxidase(NOX),a novel and efficient single-atom rhodium catalyst(Rh1/NC)was developed to mimic NOX.The Rh_(1)/NC demonstrated the ability to catalyze the dehydrogenation of NADH and transfer electrons to O_(2)to generate H_(2)O_(2)through the typical two-electron pathway.Furthermore,our findings revealed that Rh_(1)/NC exhibits the ability to catalyze the conversion of produced H_(2)O_(2)into OH under mildly acidic conditions.This process amplifies the oxidation of NADH,showcasing NADH peroxidase-like activity(NPx-like).As a paradigm,this unique dual enzyme-like property of Rh_(1)/NC with a positive feedback effect holds significance in disrupting cancer cellular homeostasis.Rh_(1)/NC can effectively consume NADH via cascade biocatalytic reactions within cancer cells,further triggering the elevation of reactive oxygen species(ROS),leading to impaired oxidative phosphorylation and decreased mitochondrial membrane potential,thus damaging the adenosine triphosphate(ATP)synthesis.The resulting'domino effect'interferes with the energy metabolism homeostasis of cancer cells,ultimately promoting cell apoptosis.This study provides potential guidance for the rational design of materials with greater capabilities.
基金Project(2025JJ70532)supported by the Natural Science Foundation of Hunan Province,ChinaProjects(21862009,21563016)supported by the National Natural Science Foundation of ChinaProject(2022GX020)supported by the Taian Science and Technology Innovation Development Project,China。
文摘Polydimethylsiloxane(PDMS)is considered a low surface energy material widely used in(super)hydrophobic modification.In this paper,the high hydrophobic melamine sponges(MS)were modified with commercial aminopropyl functionalized polydimethylsiloxane(NH_(2)-PDMS)with different molecular mass.The chemical composition,surface morphology,and wettability of the NH_(2)-PDMS-modified MS were investigated by X-ray photoelectron spectroscopy(XPS),attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR)and contact angle test.Owing to the porous structure and high hydrophobicity,NH_(2)-PDMS-modified MS possesses remarkable absorption capacity(ranging from 46 to 155 times their own mass).Simultaneously,it can effectively separate oil-water mixtures with high separation efficiencies exceeding 98.2%.NH_(2)-PDMS-modified MS has no obvious change after 10 cycles of oil-water separation.The results demonstrate PDMS molecular mass on surface can revise material properties and achieve high separation efficiencies in oil-water separation.
基金Supported by Department of Health of Jiangsu Province o China,No.H200957
文摘AIM： To investigate the association between the CpG island methylator phenotype （CIMP） and serum Helico- bacter pylori （H. pylori） levels for clinical prediction of gastric cancer （GC） progression. METHODS： We analyzed the serum ClMP status of 75 patients with GC using a methylation marker panel and a methylation-specific polymerase chain reaction. Serum samples from 40 healthy persons were examined at the same time. The genes examined were APC, WIF-1, RUNX-3, DLC-1, SFRP-1, DKK and E-cad. H. pylori infec- tion in serum was assayed with an anti-H, pylori immu- noglobulin G antibody test and a rapid urease test. RESULTS： The frequencies of high-level methylation in GC tissues for the seven genes were： 48% for APC, 57.33% for WIF-1, 56% for RUNX-3, 50.67% for DLC-1, 52% for SFRP-1, 54.67% for DKK, and 48% for E-cad.The frequencies in GC serum were 30.67% for APC, 34.67% for WIF-1, 37.33% for RUNX-3, 29.33% for DLC-1, 33.33% for SFRP-1, 32% for DKK, and 26.67% for E-cad. CIMP＋ （defined as ≥ 3 methylated genes） was associated with 47 （62.67%） GC tissue samples and 44 （58.67%） GC serum samples. CIMP＋ was not associated with non-neoplastic mucosal tissues or the serum of healthy persons. Of the 75 GC cases, 51 （68%） were H. pylori＋, and 24 （32%） were H. pylori-. Of the 51 H. pylori＋ cases, 36 were CIMP＋ and 15 were CIMP-. In contrast, for the 24 H. pylori- cases, 11 were CIMP＋, and 13 were CIMP-. The difference was signifi- cant between the H. pylori＋ and H. pylori- groups χ2 = 4.27, P 〈 0.05）. Of the 51 H. pylori＋ GC patients, 34 were CIMP＋ and 17 were CIMP-, while among the 24 H. pylori- GC cases, 10 were CIMP＋ and 14 were CIMP-. The difference was significant between the H. pylori＋ and H. pylori- groups （χ2 = 4.21, P 〈 0.05）. A 2-year follow-up showed significant difference in the rates of metastasis and recurrence between H. pylori＋/CIMP＋ cases and the H. pylori＋/CIMP- cases or CIMP- cases associated with H. pylori assayed in serum （P 〈 0.05）. However, there were no significant differences in sur- vival rates between the two groups. CONCLUSION： H. pylori＋/CIMP＋ cases are associ- ated with higher rates of metastasis and recurrence thanH, pylori＋/CIMP- cases. Serum may be useful for examining CIMP status.
文摘The earliest and most accurate detection of the pathological manifestations of hepatic diseases ensures effective treatments and thus positive prognostic outcomes.In clinical settings,screening and determining the extent of a pathology are prominent factors in preparing remedial agents and administering approp-riate therapeutic procedures.Moreover,in a patient undergoing liver resection,a realistic preoperative simulation of the subject-specific anatomy and physiology also plays a vital part in conducting initial assessments,making surgical decisions during the procedure,and anticipating postoperative results.Conventionally,various medical imaging modalities,e.g.,computed tomography,magnetic resonance imaging,and positron emission tomography,have been employed to assist in these tasks.In fact,several standardized procedures,such as lesion detection and liver segmentation,are also incorporated into prominent commercial software packages.Thus far,most integrated software as a medical device typically involves tedious interactions from the physician,such as manual delineation and empirical adjustments,as per a given patient.With the rapid progress in digital health approaches,especially medical image analysis,a wide range of computer algorithms have been proposed to facilitate those procedures.They include pattern recognition of a liver,its periphery,and lesion,as well as pre-and postoperative simulations.Prior to clinical adoption,however,software must conform to regulatory requirements set by the governing agency,for instance,valid clinical association and analytical and clinical validation.Therefore,this paper provides a detailed account and discussion of the state-of-the-art methods for liver image analyses,visualization,and simulation in the literature.Emphasis is placed upon their concepts,algorithmic classifications,merits,limitations,clinical considerations,and future research trends.
基金supported by Shandong Provincial Natural Science Foundation General Program(ZR2022MH226).
文摘Senescence,a multifaceted cellular process,intricately regulates organismal aging by imposing irreversible growth arrest on cells.This phenomenon,characterized by altered gene expression and the accumulation of senescent cells,significantly contributes to age-related physiological decline and the onset of various age-associated pathologies[1].Cellular senescence,the irreversible cessation of cell division,is intricately linked to the aging process in individuals[2].As organisms age,the accumulation of senescent cells increases,contributing to tissue dysfunction and the development of age-related pathologies.Understanding the mechanisms underlying cellular senescence holds promise for elucidating the fundamental processes governing aging and may pave the way for targeted interventions to mitigate age-associated decline[3].
基金financially supported by the Natural Science Foundation of Shandong Province(ZR2022QB014)Higher Education Institutions Youth Innovation Team Plan of Shandong Province(2022KJ192)+3 种基金Academic Promotion Program of Shandong First Medical University(2019QL009)Science and Technology Funding from Jinan(2020GXRC018)Talent Introduction Project of Shandong First Medical University(003067)High-level University and High-level Discipline Construction Project of Shandong First Medical University(923002011).
文摘Benefiting from the unique advantages of superior biocompatibility,strong stability,good biodegradability,and adjustable mechanical properties,hydrogels have attracted extensive research interests in bioelectronics.However,due to the existence of an interface between hydrogels and human tissues,the transmission of electrical signals from the human tissues to the hydrogel electronic devices will be hindered.The adhesive hydrogels with adhesive properties can tightly combine with the human tissue,which can enhance the contact between the electronic devices and human tissues and reduce the contact resistance,thereby improving the performance of hydrogel electronic devices.In this review,we will discuss in detail the adhesion mechanism of adhesive hydrogels and elaborate on the design principles of adhesive hydrogels.After that,we will introduce some methods of performance evaluation for adhesive hydrogels.Finally,we will provide a perspective on the development of adhesive hydrogel bioelectronics.
基金supported by the Natural Science Foundation of Jiangsu Province(BK20230117)the Natural Science Research Project of Nanjing Polytechnic Institute(NJPI-2023-04)+1 种基金the Startup Fund for Advanced Talents of Putian University(Grant No.2021070)the Qinglan Project of Jiangsu Province.
文摘Wound healing is a critical physiological process in living organisms that includes repair cell proliferation and migration,etc.[1,2].While effective wound-healing treatment strategies have been developed in the past,few have actively controlled the behavior of skin cells to accelerate wound healing[3,4].The importance of accelerated wound healing lies in its ability to shorten treatment time,reduce the risk of infection,and lower the cost of treatment.Electrical stimulation can modulate cell behavior during wound healing by simulating endogenous electric fields,boosting cell migration,fibroblast proliferation,and granulation tissue growth more significantly than traditional methods[5-7].However,electrical stimulation devices’size,space limitations,and unsustainability make them challenging to use in clinical surgery,hindering real-time and convenient treatment.It is therefore crucial to explore the miniaturization of electrical stimulation devices to advance the healing of skin wounds in clinical procedures.Recently,Chen et al.report a flexible micro-Zn-MnO2(mZMB)battery-based woundplast for wound management,which shows a promising path in biomedical applications(Figure 1)[8].
基金supported by the National Natural Science Foundation of China(Grant No.81972128 to Xuesong Zhang)National Natural Science Foundation of China(Grant No.82072478 to Yunpeng Zhao)the Application of Clinical Features of Capital City of Science and Technology Commission China BEIJING Special Subject(Z181100001718180 to Xuesong Zhang).
文摘Osteoarthritis(OA)has emerged as a significant health concern among the elderly population,with increasing attention paid to ferroptosis-induced OA in recent years.However,the prolonged use of nonsteroidal anti-inflammatory drugs or corticosteroids can lead to a series of side effects and limited therapeutic efficacy.This study aimed to employ the Mannich condensation reaction between epigallocatechin-3-gallate(EGCG)and selenomethionine(SeMet)to efficiently synthesize polyphenol-based nanodrugs in aqueous media for treating OA.Molecular biology experiments demonstrated that EGCG-based nanodrugs(ES NDs)could effectively reduce glutathione peroxidase 4(GPX4)inactivation,abnormal Fe2+accumulation,and lipid peroxidation induced by oxidative stress,which ameliorated the metabolic disorder of chondrocytes and other multiple pathological processes triggered by ferroptosis.Moreover,imaging and histopathological analysis of the destabilization of the medial meniscus model in mice confirmed that ES NDs exhibiting significant therapeutic effects in relieving OA.The intra-articular delivery of ES NDs represents a promising approach for treating OA and other joint inflammatory diseases.
基金supported by the National Natural Science Foundation of China(Grant No.30128018)the National Institutes of Health,USA(Grant No.R21AA13122).
文摘Estrogen receptor (ER), one member of nuclear hormone receptor (NR) family, is an estrogen-dependent transcriptional factor that plays an important role in development, progression and treatment of breast cancer. Transcriptional co-factors (co-activators and co-repressors) are critical for ER to transduce hormone and metabolic signaling to target genes. A number of functional and structural studies have elucidated the precise mechanisms of co-activator interaction with the ligand-inducible activation domain in ER via one and several LXXLL motifs (where X is any amino acid) known as NR-Box. By the yeast two-hybrid system we have identified a novel ER-a interacting protein ERIAP (Estrogen Receptor Interacting and Activating Protein) which contains two consensus LXXLL motifs. ERIAP associated with ER-a in a ligand-dependent manner, as demonstrated by in vivo immunoprecipitation and in vitro GST capture assays. The two NR boxes were essential for ERIAP interaction with ER-a. Furthermore, ERIAP specifically enhanced ligand-mediated ER-a transcriptional activity in a dose-dependent fasion and increased the expression of estrogen-responsive gene pS2. Thus, our present findings indicate that ERIAP funcions as a new coactivator for ER-a transcriptional activity, which may play an important role in development and progression of breast cancer.
基金funded by the Department of Science and Technology of Shandong ProvinceNatural Science Foundation of Shandong Province(ZR2023QH109)Taishan Scholars Program of Shandong Province(TS201712065)。
文摘Background Attention-deficit/hyperactivity disorder(ADHD)is a common neurodevelopmental disorder with behavioural symptoms and grey matter volume(GMV)changes.However,previous studies have not fully elucidated the progressive and causal GMV changes associated with behavioural symptoms in ADHD.Aims This study aimed to explore the causal relationship between GMV alterations and behavioural symptoms in children and adolescents with ADHD using behaviourcausal structural covariance network(BCaSCN)analysis.Methods Structural magnetic resonance imaging(sMRI)data from 135 children and adolescents with ADHD and182 neurotypical controls(NCs)were analysed.ADHD subtypes were identified based on GMV using a clustering algorithm to address the neuroanatomical heterogeneity.To investigate the causal relationships of GMV changes related to behavioural symptoms,sMRI data were sequentially ordered by ADHD index,inattentive index and hyperactive/impulsive index values to generate pseudotime series data.These data were then analysed using region-of-interest-based BCaSCN analysis to explore potential progressive patterns of GMV change.Results Neuroanatomical subtyping revealed two ADHD subtypes with distinct GMV patterns compared with NCs.BCaSCN analysis showed that ADHD subtype 1 was closely associated with inattentiveness,involving prominent nodes in the frontal regions and cerebellum.In contrast,ADHD subtype 2 was more strongly linked to overall disease severity,with the cerebellum and hippocampus as primary hubs.Conclusions ADHD is associated with heterogeneous changes in GMV corresponding to distinct behavioural domains,highlighting the need for subtype-specific diagnostic and therapeutic strategies.
基金the National Natural Science Foundation of China(Nos.T2241002 and 32271298)the National Key Research and Development Program of China(No.2021YFA1200402)+1 种基金the opening grants of Innovation laboratory of Terahertz Biophysics(No.23-163-00-GZ-001-001-02-01)Wenzhou Institute of the University of Chinese Academy of Sciences(WIUCASQD2021003 and WIUCASQD20210011)for support.
文摘Middle infrared stimulation(MIRS)and vibrational strong coupling(VSC)have been separately applied to physically regulate biological systems but scarcely compared with each other,especially at identical vibrational frequencies,though they both involve resonant mechanism.Taking cell proliferation and migration as typical cell-level models,herein,we comparatively studied the nonthermal bioeffects of MIRS and VSC with selecting the identical frequency(53.5 THz)of the carbonyl vibration.We found that both MIRS and VSC can notably increase the proliferation rate and migration capacity of fibroblasts.Transcriptome sequencing results reflected the differential expression of genes related to the corresponding cellular pathways.This work not only sheds light on the synergistic nonthermal bioeffects from the molecular level to the cell level but also provides new evidence and insights for modifying bioreactions,further applying MIRS and VSC to the future medicine of frequencies.
