Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects ...Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects of AI technology in molecular biology experimental teaching,this paper discusses the application of AI technology in molecular biology experimental teaching,focusing on the construction and application of virtual laboratories.At the same time,the advantages,challenges and future development directions of AI technology application are analyzed.The study found that AI technology has broad application prospects in molecular biology experimental teaching.AI technology can overcome many limitations in traditional experimental teaching,and can also provide personalized learning experience,real-time feedback and evaluation,and simulate complex molecular processes.However,the application of AI technology also faces challenges such as technology cost,teacher training,and curriculum design.In summary,the application of AI technology in molecular biology experimental teaching has significant advantages and can effectively improve teaching quality and learning effects.In the future,we should strengthen the integration of AI technology and traditional teaching methods,develop more AI teaching tools suitable for the characteristics of molecular biology,and focus on cultivating students’practical ability and innovative thinking.This study provides new ideas and directions for promoting the reform and innovation of molecular biology experimental teaching.展开更多
Alzheimer’s disease(AD)is a degenerative disease of the central nervous system.It results in cognitive dysfunction due to the loss of functional neurons and a deficit of new neurons,which can lead to death in severe ...Alzheimer’s disease(AD)is a degenerative disease of the central nervous system.It results in cognitive dysfunction due to the loss of functional neurons and a deficit of new neurons,which can lead to death in severe cases.Repairing damaged neurons by promoting hippocampal neurogenesis has become therapeutic modality to combat neurodegenerative diseases.The novel isoflavone alkaloid LY01,found in the edible fruits of Sophora alopecuroides L.,has various neuroprotective effects.However,the molecular mechanism by which it promotes the proliferation of endogenous neural stem cells(NSCs)is not yet precisely known.The effects of LY01 were investigated in vivo and in vitro.In vivo experiments showed that LY01 could counteract the toxic damage of amyloid β-protein(Aβ),enhance the learning ability and memory capacity of 5×FAD mice,and improve the morphology of neurons in the hippocampal region.In vitro experiments showed that LY01 was effective against antioxidant damage,improved the cell morphology of C17.2 mouse NSCs after hydrogen peroxide injury,and increased cell viability.Both in vitro and in vivo experiments promoted NSC proliferation by upregulating the mRNA and protein levels of the critical genes of the Wnt/β-catenin pathway,which increased levels of doublecortin to facilitate new neuronal generation.This indicates that the ability of LY01 to counteract Aβ toxicity and alleviate oxidative damage in early AD is associated with activation of the Wnt/β-catenin signaling pathway to promote NSC proliferation and thus repair damaged neurons.展开更多
AIM:To evaluate the prevalence of myopia and identify its associated risk factors among children and adolescents in Xi’an,China.METHODS:In a school-based cross-sectional design,students ranging from kindergarten to h...AIM:To evaluate the prevalence of myopia and identify its associated risk factors among children and adolescents in Xi’an,China.METHODS:In a school-based cross-sectional design,students ranging from kindergarten to high school across Xi’an were enrolled.Ophthalmic examinations were performed to assess refractive status via non-cycloplegic refraction.Myopia was defined as SE≤-0.5 D,with stratification into mild(SE:≤-0.5 to>-3.0 D),moderate(SE:≤-3.0 to>-6.0 D),and high myopia(SE≤-6.0 D).Data on potential risk factors such as age,gender,and educational level were obtained through structured questionnaires.RESULTS:The study included 156416 participants,with 81389 boys(52.0%)and 75027 girls(48.0%).The prevalence rates of myopia were 65.67%for boys and 68.83%for girls.Data collection occurred over two consecutive years,2021 and 2022,with 78849 and 77567 participants,respectively.The prevalence of myopia was 67.65%in 2021 and decreased slightly to 66.71%in 2022.The high myopia rates were 3.85%and 3.43%for these years,respectively.Analysis revealed significant risk factors including gender,age,and educational level,with a distinct positive correlation between increased educational level and higher myopia prevalence.Notably,both genders exhibited a significant decrease in moderate myopia over the study period,with reductions of 1.7%and 1.2%.CONCLUSION:This comprehensive study underscores the substantial prevalence of myopia among school-aged children and adolescents in Xi’an,marking it as a significant public health concern.The correlation with higher educational levels suggests the need for targeted interventions aimed at myopia prevention and management.These findings contribute critically to the body of knowledge necessary for formulating effective public health strategies in Xi’an and potentially other similar regions.展开更多
Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger age.China,in particular,bears a high burden of stroke cases.In recent years,the inflamm...Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger age.China,in particular,bears a high burden of stroke cases.In recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its treatment.This review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and astrocytes.Additionally,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell therapy.Overall,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient outcomes.Stem cell therapy may potentially become an important component of ischemic stroke treatment.展开更多
Schwann cell proliferation,migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system injury.Lithium promotes remyelination by Schwann cells and improves peripheral ne...Schwann cell proliferation,migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system injury.Lithium promotes remyelination by Schwann cells and improves peripheral nerve regeneration.However,whether lithium modulates other phenotypes of Schwann cells,especially their proliferation and migration remains elusive.In the current study,primary Schwann cells from rat sciatic nerve stumps were cultured and exposed to 0,5,10,15,or 30 mM lithium chloride(LiCl)for 24 hours.The effects of LiCl on Schwann cell proliferation and migration were examined using the Cell Counting Kit-8,5-ethynyl-2′-deoxyuridine,Transwell and wound healing assays.Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine assays showed that 5,10,15,and 30 mM LiCl significantly increased the viability and proliferation rate of Schwann cells.Transwell-based migration assays and wound healing assays showed that 10,15,and 30 mM LiCl suppressed the migratory ability of Schwann cells.Furthermore,the effects of LiCl on the proliferation and migration phenotypes of Schwann cells were mostly dose-dependent.These data indicate that lithium treatment significantly promotes the proliferation and inhibits the migratory ability of Schwann cells.This conclusion will inform strategies to promote the repair and regeneration of peripheral nerves.All of the animal experiments in this study were ethically approved by the Administration Committee of Experimental Animal Center of Nantong University,China(approval No.20170320-017)on March 2,2017.展开更多
Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investiga...Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina.We first showed that two types of quiescent MG(resting MG1 and MG2)reside in the uninjured retina.Following retinal injury,resting MG1 transitioned into an activated state expressing known reprogramming genes,while resting MG2 gave rise to rod progenitors.We further showed that retinal microglia can be categorized into three subtypes(microglia-1,microglia-2,and proliferative)and pseudotime analysis demonstrated dynamic changes in microglial status following retinal injury.Analysis of cell–cell interactions indicated extensive crosstalk between immune cells and MG,with many interactions shared among different immune cell types.Finally,we showed that inflammation activated Jak1–Stat3 signaling in MG,promoting their transition from a resting to an activated state.Our study reveals the cell heterogeneity and crosstalk of immune cells and MG in zebrafish retinal repair,and may provide valuable insights into future mammalian retina regeneration.展开更多
Sex-specific neurons play pivotal roles in regulating sexually dimorphic behaviors.In insects,the sex determination gene doublesex(dsx)establishes major sexual dimorphism of the nervous system,in which male-specific d...Sex-specific neurons play pivotal roles in regulating sexually dimorphic behaviors.In insects,the sex determination gene doublesex(dsx)establishes major sexual dimorphism of the nervous system,in which male-specific dsx^(M)promotes neuronal development,while female-specific dsx^(F)inhibits neuronal development by promoting neuronal apoptosis.In this study,we find that dsx regulates the number of dsx-expressing central neurons in Drosophila in cell-specific manners.Although dsx^(M)overall promotes an increase in the number of dsx neurons,it inhibits the emergence of specific pC1 neurons.dsx^(F)reduces the number of different pC1/pC2 subtypes,but promotes the formation of pC1d.We also find that dsx^(M)and dsx^(F)barely affect the number of some pC2 neurons.Changes in the number of pC1/pC2 neurons alter their roles in regulating different behaviors,including courtship,aggression,and locomotion.Our results illustrate the multifaceted functions of dsx in sexually dimorphic neuronal development and behaviors.展开更多
Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field face...Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field faces considerable challenges,including the continuous emergence of potential health threats,as well as novel pathogen strains and viruses.The advent and implementation of advanced technologies,such as culture strategies,genomics mining,and artificial intelligence(AI),are facilitating a paradigm shift in pharmaceutical research,introducing innovative methodologies and perspectives.The development and maturation of these technologies have enhanced the exploration of microbial-derived NPs,thereby advancing pharmaceutical research and development.This review synthesizes recent developments in this context,emphasizing their applications in pharmaceutical discovery and development.Through systematic analysis and synthesis,it provides objective insights into the promising prospects and future direction of this essential field.展开更多
Hepatitis B virus(HBV)is one of the major micro-pathogens in the progression of chronic liver infections worldwide.Despite a vaccine and anti-virus drugs used in the management of HBV infection,the prognosis and outco...Hepatitis B virus(HBV)is one of the major micro-pathogens in the progression of chronic liver infections worldwide.Despite a vaccine and anti-virus drugs used in the management of HBV infection,the prognosis and outcomes of this chronic infection remain complex and the infection can easily recur.Several parameters such as host age,viral mutations and genotypes,regional distributions,etc.have an effect on the outcome of hepatitis B infection following preventive measures and therapy around viral life cycle in the clinic.In addition,the economic status in different regions and groups of patients also affect disease progression.A costeffectiveness analysis is considered to play a critical role in the management of chronic HBV infection.This mini-review investigates the above-mentioned aspects and provides a perspective viewpoint for the management of HBV infection in the future.展开更多
The course of Principles of Landscape Art,which is a basic course of landscape major,has important functions of inheriting the excellent culture of the Chinese nation,cultivating ecological civilization awareness,and ...The course of Principles of Landscape Art,which is a basic course of landscape major,has important functions of inheriting the excellent culture of the Chinese nation,cultivating ecological civilization awareness,and establishing professional values.Based on the requirements of ideological and political education in courses in the new era,the paths for the ideological and political education in the course of Principles of Landscape Art were studied from aspects of the necessity,advantages and existing problems of ideological and political education in the course,aiming to achieve the organic integration of professional education and ideological and political education in education.展开更多
Non-alcoholic fatty liver disease(NAFLD)without special drugs shows symptoms of liver fat accumulation and steatosis in patients without alcohol intake.Ultrasound evaluation is a critical method in the early diagnosis...Non-alcoholic fatty liver disease(NAFLD)without special drugs shows symptoms of liver fat accumulation and steatosis in patients without alcohol intake.Ultrasound evaluation is a critical method in the early diagnosis of NAFLD stages as well as image processing and should be encouraged.Olive oil is an important component of the Mediterranean diet and has a beneficial role in the prevention of NAFLD progression.Physical activity and exercise can have anti-oxidant and anti-inflammatory effects to reduce liver fat and body weight via regulation of mitochondrial capacity in the development of NAFLD.Both the Mediterranean diet and physical exercise should be combined to achieve the ideal fat content reduction and weight loss in patients with NAFLD.展开更多
Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical...Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical vein endothelial cells.The role of the phosphatidylinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(m TOR)pathway in this process was explored.Results showed that PNSC promoted the recovery of endometrial hyperplasia and uterine index in postpartum mice.In vitro and in vivo experiments indicated that PNSC activated PI3K/AKT/m TOR signaling pathway,promoted the proliferation of human umbilical vein endothelial cells(HUVEC),up-regulated the expression of vascular endothelial growth factor(VEGF),and its binding to vascular endothelial growth factor receptor 2(VEGFR2).Moreover,it increased the expression of PCNA,MMP9,and Cyclin D1 in the nucleus.It can also up-regulated the secretion of hormones,such as prolactin(PRL),progesterone(P),as well as the level of VEGF in mice,and down-regulated the secretion of endothelin-1 hormones(ET-1),thereby promoting uterine involution.In conclusion,this study demonstrates that PNSC can regulate angiogenesis to promote uterine involution by activating the PI3K/AKT/mTOR pathway.展开更多
Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory...Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory bulb(OB),an early target in neurodegenerative diseases,acts as a gateway for environmental toxins and contains diverse neuronal populations with distinct roles.This study explored the cell-specific vulnerability to iron in the OB using a mouse model of intranasal administration of ferric ammonium citrate(FAC).Olfactory function was assessed through olfactory discrimination tests,while iron levels in OB tissues,cerebrospinal fluid(CSF),and serum were quantified using inductively coupled plasma mass spectrometry(ICP-MS),immunohistochemical staining,and iron assays.Transcriptomic changes and immune responses were assessed using RNA sequencing and immune cell infiltration analysis.Results showed that intranasal FAC administration impaired olfactory function,accompanied by iron deposition in the olfactory mucosa and OB,as well as damage to olfactory sensory neurons.Notably,these effects occurred without elevations in CSF or serum iron levels.OB iron accumulation activated multiple immune cells,including microglia and astrocytes,but did not trigger ferroptosis.Spatial transcriptomic sequencing of healthy adult mouse OBs revealed significant cellular heterogeneity,with an abundance of neuroglia and neurons.Among neurons,GABAergic neurons were the most prevalent,followed by glutamatergic and dopaminergic neurons,while cholinergic and serotonergic neurons were sparsely distributed.Under iron-stressed conditions,oligodendrocytes,dopaminergic neurons,and glutamatergic neurons exhibited significant damage,while GABAergic neurons remained unaffected.These findings highlight the selective vulnerability of neuronal and glial populations to iron-induced stress,offering novel insights into the loss of specific cell types in the OB during iron dysregulation.展开更多
Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB...Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB by redirecting carbon metabolism intermediates into the methylerythritol phosphate pathway.This process appears to be influenced by rising atmospheric CO_(2) partial pressure(pCO_(2)).This study examines how elevated pCO_(2) affects MIB production in Synechococcus sp.FACHB-1061 under varying nutrient conditions.The results indicate that elevated pCO_(2) boosts cyanobacterial biomass and total MIB yield,particularly in nutrient-rich environments,by reducing limitations in bioavailable inorganic carbon.In contrast,in nutrient-limited conditions,both total MIB concentration and per-cell production significantly increased with higher pCO_(2).Elevated pCO_(2) altered the activities of nitrate reductase and carbonic anhydrase,reducing impaired nitrogen assimilation and enhanced carbon fixation.The increase and compositional changes in extracellular polymeric substances suggested carbon overflow and a decline in cellular quality under insufficient nutrients.Additionally,markers of oxidative stress,such as increased superoxide dismutase activity and higher malondialdehyde levels,suggested that oxidative stress might stimulate secondary metabolite production.Metabolomic analysis revealed that critical intermediates of the Calvin cycle and glycolysis,such as glyceraldehyde-3-phosphate and acetyl-CoA,were upregulated with elevated pCO_(2) in nutrient-limited status,leading to increase of key precursors for MIB.These results highlight the complex interplay between nutrient and rising pCO_(2) in regulating cyanobacterial MIB biosynthesis.The findings suggest that conventional nutrient control strategies for mitigating cyanobacterial blooms and associated T&O issues may need reassessment under future climate scenarios.展开更多
Two novel skeleton sesquiterpenoids(1 and 6),along with four new iphionane-type sesquiterpenes(2−5)and six new cyperane-type sesquiterpenes(7−11),were isolated from the whole plant of Artemisia hedinii(A.hedinii).The ...Two novel skeleton sesquiterpenoids(1 and 6),along with four new iphionane-type sesquiterpenes(2−5)and six new cyperane-type sesquiterpenes(7−11),were isolated from the whole plant of Artemisia hedinii(A.hedinii).The two novel skeleton compounds(1 and 6)were derived from the decarbonization of iphionane and cyperane-type sesquiterpenes,respectively.Their structures were elucidated through a comprehensive analysis of spectroscopic data,including high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and 1D and 2D nuclear magnetic resonance(NMR)spectra.The absolute configurations were determined using electronic circular dichroism(ECD)spectra,single-crystal X-ray crystallographic analyses,time-dependent density functional theory(TDDFT)ECD calculation,density functional theory(DFT)NMR calculations,and biomimetic syntheses.The biomimetic syntheses of the two novel skeletons(1 and 6)were inspired by potential biogenetic pathways,utilizing a predominant eudesmane-type sesquiterpene(A)in A.hedinii as the substrate.All compounds were evaluated in LX-2 cells for their anti-hepatic fibrosis activity.Compounds 2,8,and 10 exhibited significant activity in downregulating the expression ofα-smooth muscle actin(α-SMA),a protein involved in hepatic fibrosis.展开更多
BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous cha...BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics.Different sources of MSCs or culture systems may cause heterogeneity of exosomes.AIM To define the potential of human adipose-derived MSC exosomes(hADSC-Exos)and human umbilical cord-derived MSC exosomes(hUCMSC-Exos)for improving dermal papillary cell proliferation in androgenetic alopecia.METHODS We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos.Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity.Additionally,the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.RESULTS We verified that hADSC-Exos inhibited glycogen synthase kinase-3βexpression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42,and enhanced dermal papillary cell proliferation and migration.Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase,but hADSC-Exos reversed these effects.CONCLUSION This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.展开更多
This corrigendum clarifies information in the article"PI3K/Akt/mTOR signaling orchestrates the phenotypic transition and chemo-resistance of small cell lung cancer"by Li et al.(2021).The authors regret that ...This corrigendum clarifies information in the article"PI3K/Akt/mTOR signaling orchestrates the phenotypic transition and chemo-resistance of small cell lung cancer"by Li et al.(2021).The authors regret that the image of H526 cells in the schematic illustration in Fig.4A displayed on the Webpage is wrong.The correct image for H526cells inthe schematic illustration is listed below。展开更多
Type 2 diabetes(T2D)presents a significant health challenge,underscoring the need for functional foods and nutraceutical hypoglycemic bioactive peptides for its prevention.This study investigates the potential of prot...Type 2 diabetes(T2D)presents a significant health challenge,underscoring the need for functional foods and nutraceutical hypoglycemic bioactive peptides for its prevention.This study investigates the potential of proteolytic hydrolysate from artificially cultivated Chinese giant salamander(CGS)meat,a rich protein source,as a preventive strategy for T2D.We produced a CGS meat hydrolysate(CGSh)and demonstrated its ability to inhibit the T2D drug target dipeptidyl peptidaseⅣ(DPP-Ⅳ)through in vitro assays.We identified 5 peptides(WRPPDH,WAPPSKD,IPDSPF,IPEMIF,and VPIAVPT)with high DPP-Ⅳinhibitory activity in CGSh,suggesting its potential antidiabetic effects.In vivo experiments showed that CGSh effectively reduced insulin resistance in mice induced with a high-fat diet,as evidenced by a slower increase in blood glucose levels and a decreased HOMA-IR index.16S rRNA sequencing analysis revealed that CGSh improved gut microbial homeostasis,promoting beneficial microorganisms and reducing harmful bacteria.Metabolomic analyses identified an increase in valeric acid levels and highlighted nine potential biomarker metabolites.By inhibiting metabolic pathways such as AGE-RAGE,CGSh might also prevent diabetic complications and reduce inflammation.These findings suggest that CGSh has a promising hypoglycemic effect,making it a potential functional food ingredient for T2D prevention and treatment.展开更多
Imprinted genes play a key role in regulating mammalian placental and embryonic development.Here,we generated glutaminyl-peptide cyclotransferase-knockout(Qpct^(-/-))mice utilizing the clustered regularly interspaced ...Imprinted genes play a key role in regulating mammalian placental and embryonic development.Here,we generated glutaminyl-peptide cyclotransferase-knockout(Qpct^(-/-))mice utilizing the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform and identified Qpct as a novel anti-angiogenic factor in regulating mouse placentation.Compared with Qpct^(+/+)mice,placentae and embryos(Qpct^(-/+)and Qpct^(-/-))showed significant overgrowth at embryonic Day 12.5(E12.5),E15.5,and E18.5.Using single-cell transcriptome analysis of 32309 cells from Qpct^(+/+)and Qpct^(-/-)mouse placentae,we identified 13 cell clusters via single-nucleus RNA sequencing(snRNA-seq)(8880 Qpct^(+/+)and 13577 Qpct^(-/-)cells)and 20 cell clusters via single-cell RNA sequencing(scRNA-seq)(6567 Qpct^(+/+)and 3285 Qpct^(-/-)cells).Furthermore,we observed a global up-regulation of pro-angiogenic genes in the Qpct^(-/-)background.Immunohistochemistry assays revealed a notable increase in the number of blood vessels in the decidual and labyrinthine layers of E15.5 Qpct^(-/+)and Qpct^(-/-)mice.Moreover,the elevation of multiple pairs of ligand-receptor interactions was observed in decidual cells,endothelial cells,and macrophages,promoting angiogenesis and inflammatory response.Our findings indicate that loss of maternal Qpct leads to altered phenotypic characteristics of placentae and embryos and promotes angiogenesis in murine placentae.展开更多
AIM:To use two-sample Mendelian randomization(MR)method to study uveitis causal association with wet age-related macular degeneration(wAMD)risk from the genetic level.METHODS:Two-sample MR analysis was used to assess ...AIM:To use two-sample Mendelian randomization(MR)method to study uveitis causal association with wet age-related macular degeneration(wAMD)risk from the genetic level.METHODS:Two-sample MR analysis was used to assess the causal role of uveitis on wAMD risk,using the 8 genetic variants associated strongly with uveitis as instrumental variables.Besides,eight MR methods[inverse variance weighted(IVW),weighted median,MR-Egger regression,weighted mode,simple mode,robust adjusted profile score(RAPS),contamination inverse-variance weighted method,and debiased inverse-variance weighted method]were used to get the whole causal estimate for multiple instrumental single nucleotide polymorphism(SNPs).The MR analysis was based on Europeans.RESULTS:Uveitis was related to a higher risk of wAMD[odds ratio(OR):1.08,95%confidence interval(CI)1.03–1.12;P=1.03×10^(-3)]with the IVW method.No heterogeneity and directional pleiotropy were detected.On the contrary,no significant results were detected in reverse MR analysis.CONCLUSION:Uveitis is related to an increased risk of wAMD.Due to the high blindness rate of wAMD,understanding and controlling the risk factors of AMD is of great significance for reducing its incidence and early diagnosis and treatment.展开更多
基金Construction Project of Teaching Quality and Teaching Reform Engineering for Undergraduate Universities in Guangdong Province(Project No.:2023-248)First-Class Undergraduate Course in Guangdong Province-Molecular Biology Experiment 2023+1 种基金Curriculum Ideology and Politics Demonstration Project of Lingnan Normal University(Project No.:2022-6&2024-44)Construction Project of Teaching Quality and Teaching Reform Engineering of Lingnan Normal University(Project No.:2024-24)。
文摘Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects of AI technology in molecular biology experimental teaching,this paper discusses the application of AI technology in molecular biology experimental teaching,focusing on the construction and application of virtual laboratories.At the same time,the advantages,challenges and future development directions of AI technology application are analyzed.The study found that AI technology has broad application prospects in molecular biology experimental teaching.AI technology can overcome many limitations in traditional experimental teaching,and can also provide personalized learning experience,real-time feedback and evaluation,and simulate complex molecular processes.However,the application of AI technology also faces challenges such as technology cost,teacher training,and curriculum design.In summary,the application of AI technology in molecular biology experimental teaching has significant advantages and can effectively improve teaching quality and learning effects.In the future,we should strengthen the integration of AI technology and traditional teaching methods,develop more AI teaching tools suitable for the characteristics of molecular biology,and focus on cultivating students’practical ability and innovative thinking.This study provides new ideas and directions for promoting the reform and innovation of molecular biology experimental teaching.
基金supported by the National Nature Science Foundation of China(82174085)Natural Science Foundation of Inner Mongolia Autonomous Region(2019MS08032).
文摘Alzheimer’s disease(AD)is a degenerative disease of the central nervous system.It results in cognitive dysfunction due to the loss of functional neurons and a deficit of new neurons,which can lead to death in severe cases.Repairing damaged neurons by promoting hippocampal neurogenesis has become therapeutic modality to combat neurodegenerative diseases.The novel isoflavone alkaloid LY01,found in the edible fruits of Sophora alopecuroides L.,has various neuroprotective effects.However,the molecular mechanism by which it promotes the proliferation of endogenous neural stem cells(NSCs)is not yet precisely known.The effects of LY01 were investigated in vivo and in vitro.In vivo experiments showed that LY01 could counteract the toxic damage of amyloid β-protein(Aβ),enhance the learning ability and memory capacity of 5×FAD mice,and improve the morphology of neurons in the hippocampal region.In vitro experiments showed that LY01 was effective against antioxidant damage,improved the cell morphology of C17.2 mouse NSCs after hydrogen peroxide injury,and increased cell viability.Both in vitro and in vivo experiments promoted NSC proliferation by upregulating the mRNA and protein levels of the critical genes of the Wnt/β-catenin pathway,which increased levels of doublecortin to facilitate new neuronal generation.This indicates that the ability of LY01 to counteract Aβ toxicity and alleviate oxidative damage in early AD is associated with activation of the Wnt/β-catenin signaling pathway to promote NSC proliferation and thus repair damaged neurons.
基金Supported by Key R&D Plan of Shaanxi Province:Key Industrial Innovation Chain(Cluster)-Social Development Field(No.2022ZDLSF03-10)the 2025 Youth Training Project of the Xi’an Municipal Health Commission(No.2025qn05)+1 种基金Xi’an Medical Research-Discipline Capacity Building Project(No.23YXYJ0002)Research Incubation Fund of Xi’an People’s Hospital(Xi’an Fourth Hospital)(No.LH-13).
文摘AIM:To evaluate the prevalence of myopia and identify its associated risk factors among children and adolescents in Xi’an,China.METHODS:In a school-based cross-sectional design,students ranging from kindergarten to high school across Xi’an were enrolled.Ophthalmic examinations were performed to assess refractive status via non-cycloplegic refraction.Myopia was defined as SE≤-0.5 D,with stratification into mild(SE:≤-0.5 to>-3.0 D),moderate(SE:≤-3.0 to>-6.0 D),and high myopia(SE≤-6.0 D).Data on potential risk factors such as age,gender,and educational level were obtained through structured questionnaires.RESULTS:The study included 156416 participants,with 81389 boys(52.0%)and 75027 girls(48.0%).The prevalence rates of myopia were 65.67%for boys and 68.83%for girls.Data collection occurred over two consecutive years,2021 and 2022,with 78849 and 77567 participants,respectively.The prevalence of myopia was 67.65%in 2021 and decreased slightly to 66.71%in 2022.The high myopia rates were 3.85%and 3.43%for these years,respectively.Analysis revealed significant risk factors including gender,age,and educational level,with a distinct positive correlation between increased educational level and higher myopia prevalence.Notably,both genders exhibited a significant decrease in moderate myopia over the study period,with reductions of 1.7%and 1.2%.CONCLUSION:This comprehensive study underscores the substantial prevalence of myopia among school-aged children and adolescents in Xi’an,marking it as a significant public health concern.The correlation with higher educational levels suggests the need for targeted interventions aimed at myopia prevention and management.These findings contribute critically to the body of knowledge necessary for formulating effective public health strategies in Xi’an and potentially other similar regions.
基金supported by grants from the Major Program of National Key Research and Development Project,Nos.2020YFA0112600(to ZH)the National Natural Science Foundation of China,No.82171270(to ZL)+5 种基金Public Service Platform for Artificial Intelligence Screening and Auxiliary Diagnosis for the Medical and Health Industry,Ministry of Industry and Information Technology of the People’s Republic of China,No.2020-0103-3-1(to ZL)the Natural Science Foundation of Beijing,No.Z200016(to ZL)Beijing Talents Project,No.2018000021223ZK03(to ZL)Beijing Municipal Committee of Science and Technology,No.Z201100005620010(to ZL)CAMS Innovation Fund for Medical Sciences,No.2019-I2M-5-029(to YW)Shanghai Engineering Research Center of Stem Cells Translational Medicine,No.20DZ2255100(to ZH).
文摘Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger age.China,in particular,bears a high burden of stroke cases.In recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its treatment.This review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and astrocytes.Additionally,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell therapy.Overall,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient outcomes.Stem cell therapy may potentially become an important component of ischemic stroke treatment.
基金supported by the National Natural Science Foundation of China,No.81970820(to HX)
文摘Schwann cell proliferation,migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system injury.Lithium promotes remyelination by Schwann cells and improves peripheral nerve regeneration.However,whether lithium modulates other phenotypes of Schwann cells,especially their proliferation and migration remains elusive.In the current study,primary Schwann cells from rat sciatic nerve stumps were cultured and exposed to 0,5,10,15,or 30 mM lithium chloride(LiCl)for 24 hours.The effects of LiCl on Schwann cell proliferation and migration were examined using the Cell Counting Kit-8,5-ethynyl-2′-deoxyuridine,Transwell and wound healing assays.Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine assays showed that 5,10,15,and 30 mM LiCl significantly increased the viability and proliferation rate of Schwann cells.Transwell-based migration assays and wound healing assays showed that 10,15,and 30 mM LiCl suppressed the migratory ability of Schwann cells.Furthermore,the effects of LiCl on the proliferation and migration phenotypes of Schwann cells were mostly dose-dependent.These data indicate that lithium treatment significantly promotes the proliferation and inhibits the migratory ability of Schwann cells.This conclusion will inform strategies to promote the repair and regeneration of peripheral nerves.All of the animal experiments in this study were ethically approved by the Administration Committee of Experimental Animal Center of Nantong University,China(approval No.20170320-017)on March 2,2017.
基金supported by the National Natural Science Foundation of China,Nos.81970820(to HX),31771644(to JL),31930068(to JL),82371176(to JL),81801331(to LC)National Key Research and Development Project of China.Nos.2017YFA0104100(to JL),2017YFA0701304(to HX)+1 种基金Shanghai Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center)Talent Introduction Plan,No.KYPT202204(to LC)the Fundamental Research Funds for the Central Universities,No.22120230292(to JL)。
文摘Inflammation plays a crucial role in the regeneration of fish and avian retinas.However,how inflammation regulates Müller glia(MG)reprogramming remains unclear.Here,we used single-cell RNA sequencing to investigate the cell heterogeneity and interactions of MG and immune cells in the regenerating zebrafish retina.We first showed that two types of quiescent MG(resting MG1 and MG2)reside in the uninjured retina.Following retinal injury,resting MG1 transitioned into an activated state expressing known reprogramming genes,while resting MG2 gave rise to rod progenitors.We further showed that retinal microglia can be categorized into three subtypes(microglia-1,microglia-2,and proliferative)and pseudotime analysis demonstrated dynamic changes in microglial status following retinal injury.Analysis of cell–cell interactions indicated extensive crosstalk between immune cells and MG,with many interactions shared among different immune cell types.Finally,we showed that inflammation activated Jak1–Stat3 signaling in MG,promoting their transition from a resting to an activated state.Our study reveals the cell heterogeneity and crosstalk of immune cells and MG in zebrafish retinal repair,and may provide valuable insights into future mammalian retina regeneration.
基金supported by the National Key R&D Program of China(2021YFA1101300)the National Natural Science Foundation of China(32371067 to Y.P.)+1 种基金the Natural Science Foundation from Jiangsu Province(BK20231418 to Q.P.)the Fundamental Research Funds for the Central Universities(2242023R40054 to Q.P.).
文摘Sex-specific neurons play pivotal roles in regulating sexually dimorphic behaviors.In insects,the sex determination gene doublesex(dsx)establishes major sexual dimorphism of the nervous system,in which male-specific dsx^(M)promotes neuronal development,while female-specific dsx^(F)inhibits neuronal development by promoting neuronal apoptosis.In this study,we find that dsx regulates the number of dsx-expressing central neurons in Drosophila in cell-specific manners.Although dsx^(M)overall promotes an increase in the number of dsx neurons,it inhibits the emergence of specific pC1 neurons.dsx^(F)reduces the number of different pC1/pC2 subtypes,but promotes the formation of pC1d.We also find that dsx^(M)and dsx^(F)barely affect the number of some pC2 neurons.Changes in the number of pC1/pC2 neurons alter their roles in regulating different behaviors,including courtship,aggression,and locomotion.Our results illustrate the multifaceted functions of dsx in sexually dimorphic neuronal development and behaviors.
基金supported by Nanjing Science and Technology Bureau International,Hong Kong,Macao,and Taiwan Science and Technology Cooperation Program(No.202401042)。
文摘Microorganisms,abundant in nature,are prolific producers of a diverse array of natural products(NPs)that are fundamental in the development of innovative therapeutics.Despite their significant potential,the field faces considerable challenges,including the continuous emergence of potential health threats,as well as novel pathogen strains and viruses.The advent and implementation of advanced technologies,such as culture strategies,genomics mining,and artificial intelligence(AI),are facilitating a paradigm shift in pharmaceutical research,introducing innovative methodologies and perspectives.The development and maturation of these technologies have enhanced the exploration of microbial-derived NPs,thereby advancing pharmaceutical research and development.This review synthesizes recent developments in this context,emphasizing their applications in pharmaceutical discovery and development.Through systematic analysis and synthesis,it provides objective insights into the promising prospects and future direction of this essential field.
基金Supported by Natural Science Foundation of Shanghai,No.17ZR1431400.
文摘Hepatitis B virus(HBV)is one of the major micro-pathogens in the progression of chronic liver infections worldwide.Despite a vaccine and anti-virus drugs used in the management of HBV infection,the prognosis and outcomes of this chronic infection remain complex and the infection can easily recur.Several parameters such as host age,viral mutations and genotypes,regional distributions,etc.have an effect on the outcome of hepatitis B infection following preventive measures and therapy around viral life cycle in the clinic.In addition,the economic status in different regions and groups of patients also affect disease progression.A costeffectiveness analysis is considered to play a critical role in the management of chronic HBV infection.This mini-review investigates the above-mentioned aspects and provides a perspective viewpoint for the management of HBV infection in the future.
基金Sponsored by the Project of Teaching Quality and Teaching Reform of Undergraduate universities in Guangdong Province[Yuejiaogaohan(2023)4].
文摘The course of Principles of Landscape Art,which is a basic course of landscape major,has important functions of inheriting the excellent culture of the Chinese nation,cultivating ecological civilization awareness,and establishing professional values.Based on the requirements of ideological and political education in courses in the new era,the paths for the ideological and political education in the course of Principles of Landscape Art were studied from aspects of the necessity,advantages and existing problems of ideological and political education in the course,aiming to achieve the organic integration of professional education and ideological and political education in education.
基金Supported by the Natural Science Foundation of Shanghai,No.17ZR1431400National Key Research and Development Program of China,No.2017YFA0103902.
文摘Non-alcoholic fatty liver disease(NAFLD)without special drugs shows symptoms of liver fat accumulation and steatosis in patients without alcohol intake.Ultrasound evaluation is a critical method in the early diagnosis of NAFLD stages as well as image processing and should be encouraged.Olive oil is an important component of the Mediterranean diet and has a beneficial role in the prevention of NAFLD progression.Physical activity and exercise can have anti-oxidant and anti-inflammatory effects to reduce liver fat and body weight via regulation of mitochondrial capacity in the development of NAFLD.Both the Mediterranean diet and physical exercise should be combined to achieve the ideal fat content reduction and weight loss in patients with NAFLD.
基金supported by National Key Research and Development Plan(2023YFC3503802)Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(C12021B013)+1 种基金Yunnan Major Scientific and Technological Projects(202202AG050021)the National Key Research and Development Program(2021YFD1000201)。
文摘Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical vein endothelial cells.The role of the phosphatidylinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(m TOR)pathway in this process was explored.Results showed that PNSC promoted the recovery of endometrial hyperplasia and uterine index in postpartum mice.In vitro and in vivo experiments indicated that PNSC activated PI3K/AKT/m TOR signaling pathway,promoted the proliferation of human umbilical vein endothelial cells(HUVEC),up-regulated the expression of vascular endothelial growth factor(VEGF),and its binding to vascular endothelial growth factor receptor 2(VEGFR2).Moreover,it increased the expression of PCNA,MMP9,and Cyclin D1 in the nucleus.It can also up-regulated the secretion of hormones,such as prolactin(PRL),progesterone(P),as well as the level of VEGF in mice,and down-regulated the secretion of endothelin-1 hormones(ET-1),thereby promoting uterine involution.In conclusion,this study demonstrates that PNSC can regulate angiogenesis to promote uterine involution by activating the PI3K/AKT/mTOR pathway.
基金supported by the National Natural Science Foundation of China (32471188,32170984,82301787)。
文摘Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory bulb(OB),an early target in neurodegenerative diseases,acts as a gateway for environmental toxins and contains diverse neuronal populations with distinct roles.This study explored the cell-specific vulnerability to iron in the OB using a mouse model of intranasal administration of ferric ammonium citrate(FAC).Olfactory function was assessed through olfactory discrimination tests,while iron levels in OB tissues,cerebrospinal fluid(CSF),and serum were quantified using inductively coupled plasma mass spectrometry(ICP-MS),immunohistochemical staining,and iron assays.Transcriptomic changes and immune responses were assessed using RNA sequencing and immune cell infiltration analysis.Results showed that intranasal FAC administration impaired olfactory function,accompanied by iron deposition in the olfactory mucosa and OB,as well as damage to olfactory sensory neurons.Notably,these effects occurred without elevations in CSF or serum iron levels.OB iron accumulation activated multiple immune cells,including microglia and astrocytes,but did not trigger ferroptosis.Spatial transcriptomic sequencing of healthy adult mouse OBs revealed significant cellular heterogeneity,with an abundance of neuroglia and neurons.Among neurons,GABAergic neurons were the most prevalent,followed by glutamatergic and dopaminergic neurons,while cholinergic and serotonergic neurons were sparsely distributed.Under iron-stressed conditions,oligodendrocytes,dopaminergic neurons,and glutamatergic neurons exhibited significant damage,while GABAergic neurons remained unaffected.These findings highlight the selective vulnerability of neuronal and glial populations to iron-induced stress,offering novel insights into the loss of specific cell types in the OB during iron dysregulation.
基金supported by the National Natural Science Foundation of China(No.41977387)the National Research Foundation,Prime Minister’s Office,Singapore under its Campus for Research Excellence and Technological Enterprise(CREATE)program.
文摘Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB by redirecting carbon metabolism intermediates into the methylerythritol phosphate pathway.This process appears to be influenced by rising atmospheric CO_(2) partial pressure(pCO_(2)).This study examines how elevated pCO_(2) affects MIB production in Synechococcus sp.FACHB-1061 under varying nutrient conditions.The results indicate that elevated pCO_(2) boosts cyanobacterial biomass and total MIB yield,particularly in nutrient-rich environments,by reducing limitations in bioavailable inorganic carbon.In contrast,in nutrient-limited conditions,both total MIB concentration and per-cell production significantly increased with higher pCO_(2).Elevated pCO_(2) altered the activities of nitrate reductase and carbonic anhydrase,reducing impaired nitrogen assimilation and enhanced carbon fixation.The increase and compositional changes in extracellular polymeric substances suggested carbon overflow and a decline in cellular quality under insufficient nutrients.Additionally,markers of oxidative stress,such as increased superoxide dismutase activity and higher malondialdehyde levels,suggested that oxidative stress might stimulate secondary metabolite production.Metabolomic analysis revealed that critical intermediates of the Calvin cycle and glycolysis,such as glyceraldehyde-3-phosphate and acetyl-CoA,were upregulated with elevated pCO_(2) in nutrient-limited status,leading to increase of key precursors for MIB.These results highlight the complex interplay between nutrient and rising pCO_(2) in regulating cyanobacterial MIB biosynthesis.The findings suggest that conventional nutrient control strategies for mitigating cyanobacterial blooms and associated T&O issues may need reassessment under future climate scenarios.
基金supported from the National Natural Science Foundation of China(No.21920102003)the Key-Area Research and Development Program of Guangdong Province(No.2020B0303070002)the National Key R&D Program“Strategic Scientific and Technological Innovation Cooperation”Key Project(No.2022YFE0203600).
文摘Two novel skeleton sesquiterpenoids(1 and 6),along with four new iphionane-type sesquiterpenes(2−5)and six new cyperane-type sesquiterpenes(7−11),were isolated from the whole plant of Artemisia hedinii(A.hedinii).The two novel skeleton compounds(1 and 6)were derived from the decarbonization of iphionane and cyperane-type sesquiterpenes,respectively.Their structures were elucidated through a comprehensive analysis of spectroscopic data,including high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and 1D and 2D nuclear magnetic resonance(NMR)spectra.The absolute configurations were determined using electronic circular dichroism(ECD)spectra,single-crystal X-ray crystallographic analyses,time-dependent density functional theory(TDDFT)ECD calculation,density functional theory(DFT)NMR calculations,and biomimetic syntheses.The biomimetic syntheses of the two novel skeletons(1 and 6)were inspired by potential biogenetic pathways,utilizing a predominant eudesmane-type sesquiterpene(A)in A.hedinii as the substrate.All compounds were evaluated in LX-2 cells for their anti-hepatic fibrosis activity.Compounds 2,8,and 10 exhibited significant activity in downregulating the expression ofα-smooth muscle actin(α-SMA),a protein involved in hepatic fibrosis.
基金Supported by the Peak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai and the China Postdoctoral Science Foundation,No.2022M722409.
文摘BACKGROUND Mesenchymal stem cells(MSCs)and their secretome have significant potential in promoting hair follicle development.However,the effects of MSC therapy have been reported to vary due to their heterogeneous characteristics.Different sources of MSCs or culture systems may cause heterogeneity of exosomes.AIM To define the potential of human adipose-derived MSC exosomes(hADSC-Exos)and human umbilical cord-derived MSC exosomes(hUCMSC-Exos)for improving dermal papillary cell proliferation in androgenetic alopecia.METHODS We conducted liquid chromatography-mass spectrometry proteomic analysis of hADSC-Exos and hUCMSC-Exos.Liquid chromatography-mass spectrometry suggested that hADSC-Exos were related to metabolism and immunity.Additionally,the hADSC-Exo proteins regulated the cell cycle and other 9 functional groups.RESULTS We verified that hADSC-Exos inhibited glycogen synthase kinase-3βexpression by activating the Wnt/β-catenin signaling pathway via cell division cycle protein 42,and enhanced dermal papillary cell proliferation and migration.Excess dihydrotestosterone caused androgenetic alopecia by shortening the hair follicle growth phase,but hADSC-Exos reversed these effects.CONCLUSION This study indicated that hair development is influenced by hADSC-Exo-mediated cell-to-cell communication via the Wnt/β-catenin pathway.
文摘This corrigendum clarifies information in the article"PI3K/Akt/mTOR signaling orchestrates the phenotypic transition and chemo-resistance of small cell lung cancer"by Li et al.(2021).The authors regret that the image of H526 cells in the schematic illustration in Fig.4A displayed on the Webpage is wrong.The correct image for H526cells inthe schematic illustration is listed below。
基金funded by the Shenzhen Agricultural Development Special Fund(Fishery)Agricultural High-tech Project([2021]735)SAMR technical guarantee project(2023YJ22 and 2023YJ37)。
文摘Type 2 diabetes(T2D)presents a significant health challenge,underscoring the need for functional foods and nutraceutical hypoglycemic bioactive peptides for its prevention.This study investigates the potential of proteolytic hydrolysate from artificially cultivated Chinese giant salamander(CGS)meat,a rich protein source,as a preventive strategy for T2D.We produced a CGS meat hydrolysate(CGSh)and demonstrated its ability to inhibit the T2D drug target dipeptidyl peptidaseⅣ(DPP-Ⅳ)through in vitro assays.We identified 5 peptides(WRPPDH,WAPPSKD,IPDSPF,IPEMIF,and VPIAVPT)with high DPP-Ⅳinhibitory activity in CGSh,suggesting its potential antidiabetic effects.In vivo experiments showed that CGSh effectively reduced insulin resistance in mice induced with a high-fat diet,as evidenced by a slower increase in blood glucose levels and a decreased HOMA-IR index.16S rRNA sequencing analysis revealed that CGSh improved gut microbial homeostasis,promoting beneficial microorganisms and reducing harmful bacteria.Metabolomic analyses identified an increase in valeric acid levels and highlighted nine potential biomarker metabolites.By inhibiting metabolic pathways such as AGE-RAGE,CGSh might also prevent diabetic complications and reduce inflammation.These findings suggest that CGSh has a promising hypoglycemic effect,making it a potential functional food ingredient for T2D prevention and treatment.
基金supported by the National Natural Science Foundation of China(No.32271165)the Interdisciplinary Project in Ocean Research of Tongji University(No.2022-2-ZD-02),China.
文摘Imprinted genes play a key role in regulating mammalian placental and embryonic development.Here,we generated glutaminyl-peptide cyclotransferase-knockout(Qpct^(-/-))mice utilizing the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform and identified Qpct as a novel anti-angiogenic factor in regulating mouse placentation.Compared with Qpct^(+/+)mice,placentae and embryos(Qpct^(-/+)and Qpct^(-/-))showed significant overgrowth at embryonic Day 12.5(E12.5),E15.5,and E18.5.Using single-cell transcriptome analysis of 32309 cells from Qpct^(+/+)and Qpct^(-/-)mouse placentae,we identified 13 cell clusters via single-nucleus RNA sequencing(snRNA-seq)(8880 Qpct^(+/+)and 13577 Qpct^(-/-)cells)and 20 cell clusters via single-cell RNA sequencing(scRNA-seq)(6567 Qpct^(+/+)and 3285 Qpct^(-/-)cells).Furthermore,we observed a global up-regulation of pro-angiogenic genes in the Qpct^(-/-)background.Immunohistochemistry assays revealed a notable increase in the number of blood vessels in the decidual and labyrinthine layers of E15.5 Qpct^(-/+)and Qpct^(-/-)mice.Moreover,the elevation of multiple pairs of ligand-receptor interactions was observed in decidual cells,endothelial cells,and macrophages,promoting angiogenesis and inflammatory response.Our findings indicate that loss of maternal Qpct leads to altered phenotypic characteristics of placentae and embryos and promotes angiogenesis in murine placentae.
基金Supported by the National Natural Science Foundation of China(No.82201163)Natural Science Foundation Youth Foundation of Shaanxi Province(No.2023-JC-QN-0861)Shaanxi Province Key Research and Development Program(No.2021SF-332).
文摘AIM:To use two-sample Mendelian randomization(MR)method to study uveitis causal association with wet age-related macular degeneration(wAMD)risk from the genetic level.METHODS:Two-sample MR analysis was used to assess the causal role of uveitis on wAMD risk,using the 8 genetic variants associated strongly with uveitis as instrumental variables.Besides,eight MR methods[inverse variance weighted(IVW),weighted median,MR-Egger regression,weighted mode,simple mode,robust adjusted profile score(RAPS),contamination inverse-variance weighted method,and debiased inverse-variance weighted method]were used to get the whole causal estimate for multiple instrumental single nucleotide polymorphism(SNPs).The MR analysis was based on Europeans.RESULTS:Uveitis was related to a higher risk of wAMD[odds ratio(OR):1.08,95%confidence interval(CI)1.03–1.12;P=1.03×10^(-3)]with the IVW method.No heterogeneity and directional pleiotropy were detected.On the contrary,no significant results were detected in reverse MR analysis.CONCLUSION:Uveitis is related to an increased risk of wAMD.Due to the high blindness rate of wAMD,understanding and controlling the risk factors of AMD is of great significance for reducing its incidence and early diagnosis and treatment.
