It is predicted that the distribution on score of children＇s motor performance show abnormal curve, because their physical fitness shows decline and gap between good and poor. The aim of present study is to type patt...It is predicted that the distribution on score of children＇s motor performance show abnormal curve, because their physical fitness shows decline and gap between good and poor. The aim of present study is to type pattern of distribution curve on physique and motor performance （PMP） in preschoolers, and to investigate gender difference, and change in season and age on distribution of PMP. Seven hundred and seven preschoolers participate in measurement on 21 items of PMP. And mix-longitudinal data which made from 6 terms （2 seasons of spring and autumn for 3 years） is completed. The histograms of 10 sections are trained for each gender, term, and test item based on range of data including mean ± 3 standard deviation. By cluster analysis and multi-correspondence analysis, ＂physique ＆ jumping＂ show closely normal curve, but ＂manipulation （MP）＂ is skewed to poor and ＂running ＆ prompt （RP）＂ is skewed to good. By Hotelling＇s T2-test and Mahalanobis＇ Distance, gender difference is shown that boys＇ RP is skewed to good, and their ＂throwing＂ and ＂weight ＆ flexibility （WF）＂ are skewed to poor. About season, WF is skewed to poor and RP is skewed to good, in spring. In annual change about pattern of distribution, skewing poor in MP and good in ＂running straight ＆ prompt＂ are changed to normal curve with aging.展开更多
In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize op...In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography(OT-CEC)enantioseparation.The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters,all achieving good resolution and symmetrical peak shape.For three consecutive runs,the relative standard deviations(RSD)of migration time for intra-day,inter-day,and column-tocolumn repeatability were in the range of 0.3%-3.5%,0.2%-4.9%and 2.1%-7.7%,respectively.Moreover,the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency.Therefore,the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.展开更多
Studies on TGS （toe-grip strength） are currently proliferating as a result of the development of the dynamometer. The purpose of the present study was to investigate the reliability and validity of TGS as a physical...Studies on TGS （toe-grip strength） are currently proliferating as a result of the development of the dynamometer. The purpose of the present study was to investigate the reliability and validity of TGS as a physical function in preschool aged children. The participants were 153 preschoolers. Each participant was measured in terms of his or her TGS and completed a MAT （motor ability test）. The reliability of the measurements was investigated via Pearson＇s r and Cronbach＇s a through a test-retest method, as well as a Bland-Altman plot. The validity of the TGS value was investigated by measuring the correlation between TGS and each component of the MAT, the principal component analysis, and a two-way layout ANOVA with general linear model （gender and age）. All reliability coefficients were more than 0.70. Though all components of the MAT relating to TGS were found to be significant （P 〈 0.05）, these correlations were weak. However, TGS was found to be a physical function that relating to the lower limbs and develops with aging. Therefore, TGS was found to be a highly reliable measure of physical function performance in preschoolers.展开更多
Preoperative sleep loss can amplify post-operative mechanical hyperalgesia.However,the underlying mechanisms are still largely unknown.In the current study,rats were randomly allocated to a control group and an acute ...Preoperative sleep loss can amplify post-operative mechanical hyperalgesia.However,the underlying mechanisms are still largely unknown.In the current study,rats were randomly allocated to a control group and an acute sleep deprivation(ASD)group which experienced 6 h ASD before surgery.Then the variations in cerebral function and activity were investigated with multi-modal techniques,such as nuclear magnetic resonance,functional magnetic resonance imaging,c-Fos immunofluorescence,and electrophysiology.The results indicated that ASD induced hyperalgesia,and the metabolic kinetics were remarkably decreased in the striatum and midbrain.The functional connectivity(FC)between the nucleus accumbens(NAc,a subregion of the ventral striatum)and the ventrolateral periaqueductal gray(vLPAG)was significantly reduced,and the c-Fos expression in the NAc and the vLPAG was suppressed.Furthermore,the electrophysiological recordings demonstrated that both the neuronal activity in the NAc and the vLPAG,and the coherence of the NAc-vLPAG were suppressed in both resting and task states.This study showed that neuronal activity in the NAc and the vLPAG were weakened and the FC between the NAc and the vLPAG was also suppressed in rats with ASD-induced hyperalgesia.This study highlights the importance of preoperative sleep management for surgical patients.展开更多
Abnormal postoperative neurobehavioral performance(APNP)is a common phenomenon in the early postoperative period.The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a s...Abnormal postoperative neurobehavioral performance(APNP)is a common phenomenon in the early postoperative period.The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP.The dynamic changes of metabolites in different brain regions after anesthesia and surgery,as well as their potential association with APNP are still not well understood.Here,we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice,and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance('H-NMR)spectroscopy.The abnormal neurobehavioral performance occurred at 6 h and/or 9 h,and recovered at 24 h after anesthesia/surgery.Compared with the control group,the altered metabolite of the model group at 6 h was aspartate(Asp),and the difference was mainly displayed in the cortex;while significant changes at 9 h occurred predominantly in the cortex and hippocampus,and the corresponding metabolites were Asp and glutamate(Glu).All changes returned to baseline at 24 h.The altered metabolic changes could have occurred as a result of the acute APNP,and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.展开更多
Background and Objective Since its initial report by James Parkinson in 1817,Parkinson’s disease(PD)has remained a central subject of research and clinical advancement.The disease is estimated to affect approximately...Background and Objective Since its initial report by James Parkinson in 1817,Parkinson’s disease(PD)has remained a central subject of research and clinical advancement.The disease is estimated to affect approximately 1%of adults aged 60 and above.Deep brain stimulation,emerging as an alternative therapy for end-stage cases,has offered a lifeline to numerous patients.This review aimed to analyze publications pertaining to the impact of deep brain stimulation on the motor pathway in patients with PD over the last decade.Methods Data were obtained from the Web of Science Core Collection through the library of Huazhong University of Science and Technology(China).The search strategy encompassed the following keywords:“deep brain stimulation”,“Parkinson’s disease”,“motor pathway”,and“human”,from January 1,2012,to December 1,2022.Additionally,this review visualized the findings using the Citespace software.Results The results indicated that the United States,the United Kingdom,Germany,and China were the primary contributors to this research field.University College London,Capital Medical University,and Maastricht University were the top 3 research institutions in the research area.Tom Foltynie ranked first with 6 publications,and the journals of Brain and Brain Stimulation published the greatest number of relevant articles.The prevailing research focal points in this domain,as determined by keywords“burst analysis”,“encompassed neuronal activity”,“nucleus”,“hyper direct pathway”,etc.Conclusion This study has provided a new perspective through bibliometric analysis of the deep brain stimulation therapy for treating patients with PD,which can shed light on future research to advance our comprehension of this particular field of study.展开更多
The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volat...The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.展开更多
The aim of the present study was to investigate the relationship between stress response, gender, weight management, and physical exercise among Japanese university students. The participants were 411 university stude...The aim of the present study was to investigate the relationship between stress response, gender, weight management, and physical exercise among Japanese university students. The participants were 411 university students （169 males, 242 females）. All participants completed the Stress Response Scale and the European Health and Behavior Survey. T-test results showed that scores of females were consistently higher than those of males （P 〈 0.05）. In the chi-square test, the proportion of females managing their weight （75.3%） was significantly higher than that of males （42.9%）. In multiple regression analysis, the group managing their weight showed a higher stress score, and the group doing physical exercise showed a lower stress score. These results show that weight management through physical exercise reduces mental stress, making it the most beneficial method of weight management for the mental and physical wellbeing of youths.展开更多
Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseu...Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits.展开更多
This article aims to identify barriers to implementing waste management practices in construction projects and their interrelationship,based on the particular context of Australia.Interviews and a questionnaire survey...This article aims to identify barriers to implementing waste management practices in construction projects and their interrelationship,based on the particular context of Australia.Interviews and a questionnaire survey were conducted as the primary data collection methods supported by the findings of a charrette.The findings reveal twenty critical barriers to implementing waste management practices in Australian construction projects.Four underlying factors that impede waste management prac-tices are extracted based on results of an exploratory factor analysis.These include rigidity of construction practices,construction project characteristics,awareness,experience and commitment,and the nascent nature of waste management.The study also finds that while both human factors and technical factors act as barriers to implementing waste management practices in Australian construction projects,human factors are more dominant.Thus,it is essential to address all these barriers in the early stage of construction projects for reducing waste generation.展开更多
文摘It is predicted that the distribution on score of children＇s motor performance show abnormal curve, because their physical fitness shows decline and gap between good and poor. The aim of present study is to type pattern of distribution curve on physique and motor performance （PMP） in preschoolers, and to investigate gender difference, and change in season and age on distribution of PMP. Seven hundred and seven preschoolers participate in measurement on 21 items of PMP. And mix-longitudinal data which made from 6 terms （2 seasons of spring and autumn for 3 years） is completed. The histograms of 10 sections are trained for each gender, term, and test item based on range of data including mean ± 3 standard deviation. By cluster analysis and multi-correspondence analysis, ＂physique ＆ jumping＂ show closely normal curve, but ＂manipulation （MP）＂ is skewed to poor and ＂running ＆ prompt （RP）＂ is skewed to good. By Hotelling＇s T2-test and Mahalanobis＇ Distance, gender difference is shown that boys＇ RP is skewed to good, and their ＂throwing＂ and ＂weight ＆ flexibility （WF）＂ are skewed to poor. About season, WF is skewed to poor and RP is skewed to good, in spring. In annual change about pattern of distribution, skewing poor in MP and good in ＂running straight ＆ prompt＂ are changed to normal curve with aging.
基金the financial support from the National Natural Science Foundation of China(No.21874060)the Special Funding for Open and Shared Large-Scale Instruments and Equipments of Lanzhou University(No.LZU-GXJJ-2019C038)。
文摘In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography(OT-CEC)enantioseparation.The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters,all achieving good resolution and symmetrical peak shape.For three consecutive runs,the relative standard deviations(RSD)of migration time for intra-day,inter-day,and column-tocolumn repeatability were in the range of 0.3%-3.5%,0.2%-4.9%and 2.1%-7.7%,respectively.Moreover,the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency.Therefore,the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.
文摘Studies on TGS （toe-grip strength） are currently proliferating as a result of the development of the dynamometer. The purpose of the present study was to investigate the reliability and validity of TGS as a physical function in preschool aged children. The participants were 153 preschoolers. Each participant was measured in terms of his or her TGS and completed a MAT （motor ability test）. The reliability of the measurements was investigated via Pearson＇s r and Cronbach＇s a through a test-retest method, as well as a Bland-Altman plot. The validity of the TGS value was investigated by measuring the correlation between TGS and each component of the MAT, the principal component analysis, and a two-way layout ANOVA with general linear model （gender and age）. All reliability coefficients were more than 0.70. Though all components of the MAT relating to TGS were found to be significant （P 〈 0.05）, these correlations were weak. However, TGS was found to be a physical function that relating to the lower limbs and develops with aging. Therefore, TGS was found to be a highly reliable measure of physical function performance in preschoolers.
基金This work was supported by grants from the National Natural Science Foundation of China(82071208,81870851,31771193,and 81971775)the Outstanding Talented Young Doctor Program of Hubei Province(HB20200407)+2 种基金the Translational Medicine,and interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(ZNJC202012)the Medical Sci-Tech Innovation Platform of Zhongnan Hospital of Wuhan Universitythe Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200).
文摘Preoperative sleep loss can amplify post-operative mechanical hyperalgesia.However,the underlying mechanisms are still largely unknown.In the current study,rats were randomly allocated to a control group and an acute sleep deprivation(ASD)group which experienced 6 h ASD before surgery.Then the variations in cerebral function and activity were investigated with multi-modal techniques,such as nuclear magnetic resonance,functional magnetic resonance imaging,c-Fos immunofluorescence,and electrophysiology.The results indicated that ASD induced hyperalgesia,and the metabolic kinetics were remarkably decreased in the striatum and midbrain.The functional connectivity(FC)between the nucleus accumbens(NAc,a subregion of the ventral striatum)and the ventrolateral periaqueductal gray(vLPAG)was significantly reduced,and the c-Fos expression in the NAc and the vLPAG was suppressed.Furthermore,the electrophysiological recordings demonstrated that both the neuronal activity in the NAc and the vLPAG,and the coherence of the NAc-vLPAG were suppressed in both resting and task states.This study showed that neuronal activity in the NAc and the vLPAG were weakened and the FC between the NAc and the vLPAG was also suppressed in rats with ASD-induced hyperalgesia.This study highlights the importance of preoperative sleep management for surgical patients.
基金We would like to express our gratitude to Mrs.Pingping An(Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences)for her help in housing the animals.This work was supported by grants from the National Natural Science Foundation of China(8187051484,8157050329,81600933)the Interdisciplinary Medicine Seed Fund of Peking University(BMU2017MC006)the Youth Innovation Promotion Association of the Chinese Academy of Sciences,China(Y6Y0021004).
文摘Abnormal postoperative neurobehavioral performance(APNP)is a common phenomenon in the early postoperative period.The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP.The dynamic changes of metabolites in different brain regions after anesthesia and surgery,as well as their potential association with APNP are still not well understood.Here,we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice,and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance('H-NMR)spectroscopy.The abnormal neurobehavioral performance occurred at 6 h and/or 9 h,and recovered at 24 h after anesthesia/surgery.Compared with the control group,the altered metabolite of the model group at 6 h was aspartate(Asp),and the difference was mainly displayed in the cortex;while significant changes at 9 h occurred predominantly in the cortex and hippocampus,and the corresponding metabolites were Asp and glutamate(Glu).All changes returned to baseline at 24 h.The altered metabolic changes could have occurred as a result of the acute APNP,and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.
基金the National Natural Science Foundation of China(No.81873467 and No.81670240).
文摘Background and Objective Since its initial report by James Parkinson in 1817,Parkinson’s disease(PD)has remained a central subject of research and clinical advancement.The disease is estimated to affect approximately 1%of adults aged 60 and above.Deep brain stimulation,emerging as an alternative therapy for end-stage cases,has offered a lifeline to numerous patients.This review aimed to analyze publications pertaining to the impact of deep brain stimulation on the motor pathway in patients with PD over the last decade.Methods Data were obtained from the Web of Science Core Collection through the library of Huazhong University of Science and Technology(China).The search strategy encompassed the following keywords:“deep brain stimulation”,“Parkinson’s disease”,“motor pathway”,and“human”,from January 1,2012,to December 1,2022.Additionally,this review visualized the findings using the Citespace software.Results The results indicated that the United States,the United Kingdom,Germany,and China were the primary contributors to this research field.University College London,Capital Medical University,and Maastricht University were the top 3 research institutions in the research area.Tom Foltynie ranked first with 6 publications,and the journals of Brain and Brain Stimulation published the greatest number of relevant articles.The prevailing research focal points in this domain,as determined by keywords“burst analysis”,“encompassed neuronal activity”,“nucleus”,“hyper direct pathway”,etc.Conclusion This study has provided a new perspective through bibliometric analysis of the deep brain stimulation therapy for treating patients with PD,which can shed light on future research to advance our comprehension of this particular field of study.
基金supported by the National Natural Science Foundation of China(31400946,31771156,91632303/H09,91732304 and 31830035)Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200)。
文摘The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.
文摘The aim of the present study was to investigate the relationship between stress response, gender, weight management, and physical exercise among Japanese university students. The participants were 411 university students （169 males, 242 females）. All participants completed the Stress Response Scale and the European Health and Behavior Survey. T-test results showed that scores of females were consistently higher than those of males （P 〈 0.05）. In the chi-square test, the proportion of females managing their weight （75.3%） was significantly higher than that of males （42.9%）. In multiple regression analysis, the group managing their weight showed a higher stress score, and the group doing physical exercise showed a lower stress score. These results show that weight management through physical exercise reduces mental stress, making it the most beneficial method of weight management for the mental and physical wellbeing of youths.
基金the National Basic Research Program(973 Program)of China(2015CB755601)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200)+1 种基金the National Natural Science Foundation of China(31771156,81661148053,91632303,31800885,31500868,31671120 and 91732304)the China Postdoctoral Science Foundation(2019M653118 and 2018M632946).
文摘Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits.
基金supported by the National Natural Science Foundation of China(71573216).
文摘This article aims to identify barriers to implementing waste management practices in construction projects and their interrelationship,based on the particular context of Australia.Interviews and a questionnaire survey were conducted as the primary data collection methods supported by the findings of a charrette.The findings reveal twenty critical barriers to implementing waste management practices in Australian construction projects.Four underlying factors that impede waste management prac-tices are extracted based on results of an exploratory factor analysis.These include rigidity of construction practices,construction project characteristics,awareness,experience and commitment,and the nascent nature of waste management.The study also finds that while both human factors and technical factors act as barriers to implementing waste management practices in Australian construction projects,human factors are more dominant.Thus,it is essential to address all these barriers in the early stage of construction projects for reducing waste generation.
