Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord in...Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.展开更多
Objective To observe the clinical effect of acupuncture at Tiaoshen(regulating the spirit)acupoints on chronic insomnia and hyperarousal state,and explore its possible mechanism.Methods A total of 60 patients with chr...Objective To observe the clinical effect of acupuncture at Tiaoshen(regulating the spirit)acupoints on chronic insomnia and hyperarousal state,and explore its possible mechanism.Methods A total of 60 patients with chronic insomnia were randomly divided into an acupuncture group(30 cases,1 case dropped off)and a sham acupuncture group(30 cases,1 cases dropped off).展开更多
Objective To observe the efficacy of acupoint thread-embedding combined with fluticasone propionate aerosol for chronic persistent bronchial asthma of lungspleen qi deficiency and spleen-kidney yang deficiency in chil...Objective To observe the efficacy of acupoint thread-embedding combined with fluticasone propionate aerosol for chronic persistent bronchial asthma of lungspleen qi deficiency and spleen-kidney yang deficiency in children and its effect on pulmonary function and serum levels of IgA and IgE.Methods A total of 120 children with chronic persistent bronchial asthma were randomly divided into an observation group(60 cases,9 cases dropped off)and a control group(60 cases,7 cases dropped off).展开更多
Objective To compare the therapeutic effect of acupuncture combined with wheat-grain moxibustion and oral fluoxetine hydrochloride capsule on perimenopausal depression with kidney deficiency and liver depression.Metho...Objective To compare the therapeutic effect of acupuncture combined with wheat-grain moxibustion and oral fluoxetine hydrochloride capsule on perimenopausal depression with kidney deficiency and liver depression.Methods A total of 60 patients of perimenopausal depression with kidney deficiency and liver depression were randomized into an observation group and a control group,30 cases in each.展开更多
Objective To observe the mechanism and different effects of five blood-activating and stasis resolving prescriptions on endothelial cell function in rabbit with blood stasis syndrome.Methods Seventy New Zealand rabbit...Objective To observe the mechanism and different effects of five blood-activating and stasis resolving prescriptions on endothelial cell function in rabbit with blood stasis syndrome.Methods Seventy New Zealand rabbits were selected,6 of them were served as control group,and the remaining 64 were given hunger+hyperlipidemia+adrenaline method to establish blood stasis syndrome.The successfully established model rabbits were divided into model group (n=9).展开更多
Objective To compare the clinical effect of acupuncture combined with wheat-grain moxibustion and oral sertraline hydrochloride dispersible tablets in the treatment of mild to moderate postpartum depression.Methods Si...Objective To compare the clinical effect of acupuncture combined with wheat-grain moxibustion and oral sertraline hydrochloride dispersible tablets in the treatment of mild to moderate postpartum depression.Methods Sixty patients with mild to moderate postpartum depression were randomly divided into an observation group and a control group,30 in each group.Both groups were treated with psychotherapy.展开更多
Objective To evaluate the effects of Qingda Granules (QDG) on office blood pressure (OBP) and the anxiety score in patients with grade 1 hypertension with different levels of anxiety.Methods A randomized,multicenter,d...Objective To evaluate the effects of Qingda Granules (QDG) on office blood pressure (OBP) and the anxiety score in patients with grade 1 hypertension with different levels of anxiety.Methods A randomized,multicenter,double-blinded,non-inferiority clinical trial design was adopted.A total of 552 patients with grade 1hypertension were randomly assigned to the treatment group(276 cases) and the control group (276 cases).展开更多
Objective To analyze the mechanism of Huoxiang Zhengqi Recipe in treating novel coronavirus pneumonia in pregnancy by using network pharmacology and explore the relationship between its multiple components,targets and...Objective To analyze the mechanism of Huoxiang Zhengqi Recipe in treating novel coronavirus pneumonia in pregnancy by using network pharmacology and explore the relationship between its multiple components,targets and pathways.展开更多
Objective To observe the effects of electroacupuncture(EA) at"Jiaji"(EX-B 2) combined with neurodynamic mobilization (NM) on the cross-sectional area of the gastrocnemius muscle fibers after sciatic nerve in...Objective To observe the effects of electroacupuncture(EA) at"Jiaji"(EX-B 2) combined with neurodynamic mobilization (NM) on the cross-sectional area of the gastrocnemius muscle fibers after sciatic nerve injury in rabbits,and the expression of nuclear factor-κB (NF-κB) and muscle-specific ring-finger protein 1 (MuRF1).展开更多
Objective To screen protein target in prevention and treatment with electroacupuncture(EA)for Alzheimer's disease(AD)and explore the potential mechanism of EA in prevention of AD.Methods A total of 40 APP/PSI tran...Objective To screen protein target in prevention and treatment with electroacupuncture(EA)for Alzheimer's disease(AD)and explore the potential mechanism of EA in prevention of AD.Methods A total of 40 APP/PSI transgenic young male mice,1.5-month old,were randomized into an EA group and a model group,20 mice in each,and 20 C57BL/6J mice were chosen as normal control group.展开更多
Objective To observe the intervention effect of Banxia Xiexin Decoction (BXXXD) plasma-derived exosomes on the network structure of interstitial cells of Cajal(ICCs) in gastric antrum tissues of rats with gastric elec...Objective To observe the intervention effect of Banxia Xiexin Decoction (BXXXD) plasma-derived exosomes on the network structure of interstitial cells of Cajal(ICCs) in gastric antrum tissues of rats with gastric electric dysrhythmia and the possible mechanism of regulating gastric motility.展开更多
Objective To observe the clinical effect of wristankle acupuncture on postpartum abdominal pain and its influence on serum beta-endorphin (β-EP) level in puerpera.Methods Seventy patients with postpartum abdominal pa...Objective To observe the clinical effect of wristankle acupuncture on postpartum abdominal pain and its influence on serum beta-endorphin (β-EP) level in puerpera.Methods Seventy patients with postpartum abdominal pain were randomly divided into an acupuncture+herbal medication group (35 cases,1 case dropped out) and a herbal medication group(35 cases,2cases dropped out).In the herbal medication group.展开更多
Objective To investigate the feasibility and safety of single peroral choledochoscopy combined with radiofrequency ablation (RFA) for nonresectable extrahepatic cholangiocarcinoma (EHCC).Methods A total of 90 patients...Objective To investigate the feasibility and safety of single peroral choledochoscopy combined with radiofrequency ablation (RFA) for nonresectable extrahepatic cholangiocarcinoma (EHCC).Methods A total of 90 patients with suspected EHCC treated in Hangzhou First People's Hospital from January 2013 to January 2022 were reviewed.According to the diagnosis and treatment process,69 patients were finally included and divided into two groups.展开更多
Objective To investigate the clinical characteristics of Chinese and Western medicine in patients with severe systemic lupus erythematosus(SSLE).Methods Clinical data of SSLE inpatients in Guangdong Provincial Hospita...Objective To investigate the clinical characteristics of Chinese and Western medicine in patients with severe systemic lupus erythematosus(SSLE).Methods Clinical data of SSLE inpatients in Guangdong Provincial Hospital of Chinese Medicine from January 2011 to December 2017 were collected.The gender,age,clinical characteristics,organ damage,Chinese medicine syndrome,tongue and pulse manifestation,mortality and cause of death were statistically analyzed by retrospective study.展开更多
Objective To observe the clinical efficacy of acupuncture of revised acupoint combination around theskull base in treating post-stroke mild cognitive impairment(PSMCI),and preliminary explore its action mechanism.Meth...Objective To observe the clinical efficacy of acupuncture of revised acupoint combination around theskull base in treating post-stroke mild cognitive impairment(PSMCI),and preliminary explore its action mechanism.Methods A total of 76 PSMCI patients were randomly divided into an observation group(38 cases,4cases dropped off)and a control group(38 cases,3cases dropped off,1 case was removed).展开更多
Objective To investigate the inhibitory effect of Gandou Decoction combined with penicillamine on cuproptosis in the liver of toxic milk (TX) mice,which serve as a model for Wilson's disease (WD).Methods Using dil...Objective To investigate the inhibitory effect of Gandou Decoction combined with penicillamine on cuproptosis in the liver of toxic milk (TX) mice,which serve as a model for Wilson's disease (WD).Methods Using dilute lethal (DL) mice as the normal control group,and TX mice were randomly divided into model group,Gandou Decoction group,penicillamine group,and Gandou Decoction combined with penicillamine group,with 8 mice in each group.展开更多
文摘Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.
文摘Objective To observe the clinical effect of acupuncture at Tiaoshen(regulating the spirit)acupoints on chronic insomnia and hyperarousal state,and explore its possible mechanism.Methods A total of 60 patients with chronic insomnia were randomly divided into an acupuncture group(30 cases,1 case dropped off)and a sham acupuncture group(30 cases,1 cases dropped off).
文摘Objective To observe the efficacy of acupoint thread-embedding combined with fluticasone propionate aerosol for chronic persistent bronchial asthma of lungspleen qi deficiency and spleen-kidney yang deficiency in children and its effect on pulmonary function and serum levels of IgA and IgE.Methods A total of 120 children with chronic persistent bronchial asthma were randomly divided into an observation group(60 cases,9 cases dropped off)and a control group(60 cases,7 cases dropped off).
文摘Objective To compare the therapeutic effect of acupuncture combined with wheat-grain moxibustion and oral fluoxetine hydrochloride capsule on perimenopausal depression with kidney deficiency and liver depression.Methods A total of 60 patients of perimenopausal depression with kidney deficiency and liver depression were randomized into an observation group and a control group,30 cases in each.
文摘Objective To observe the mechanism and different effects of five blood-activating and stasis resolving prescriptions on endothelial cell function in rabbit with blood stasis syndrome.Methods Seventy New Zealand rabbits were selected,6 of them were served as control group,and the remaining 64 were given hunger+hyperlipidemia+adrenaline method to establish blood stasis syndrome.The successfully established model rabbits were divided into model group (n=9).
文摘Objective To compare the clinical effect of acupuncture combined with wheat-grain moxibustion and oral sertraline hydrochloride dispersible tablets in the treatment of mild to moderate postpartum depression.Methods Sixty patients with mild to moderate postpartum depression were randomly divided into an observation group and a control group,30 in each group.Both groups were treated with psychotherapy.
文摘Objective To evaluate the effects of Qingda Granules (QDG) on office blood pressure (OBP) and the anxiety score in patients with grade 1 hypertension with different levels of anxiety.Methods A randomized,multicenter,double-blinded,non-inferiority clinical trial design was adopted.A total of 552 patients with grade 1hypertension were randomly assigned to the treatment group(276 cases) and the control group (276 cases).
文摘Objective To analyze the mechanism of Huoxiang Zhengqi Recipe in treating novel coronavirus pneumonia in pregnancy by using network pharmacology and explore the relationship between its multiple components,targets and pathways.
文摘Objective To observe the effects of electroacupuncture(EA) at"Jiaji"(EX-B 2) combined with neurodynamic mobilization (NM) on the cross-sectional area of the gastrocnemius muscle fibers after sciatic nerve injury in rabbits,and the expression of nuclear factor-κB (NF-κB) and muscle-specific ring-finger protein 1 (MuRF1).
文摘Objective To screen protein target in prevention and treatment with electroacupuncture(EA)for Alzheimer's disease(AD)and explore the potential mechanism of EA in prevention of AD.Methods A total of 40 APP/PSI transgenic young male mice,1.5-month old,were randomized into an EA group and a model group,20 mice in each,and 20 C57BL/6J mice were chosen as normal control group.
文摘Objective To observe the intervention effect of Banxia Xiexin Decoction (BXXXD) plasma-derived exosomes on the network structure of interstitial cells of Cajal(ICCs) in gastric antrum tissues of rats with gastric electric dysrhythmia and the possible mechanism of regulating gastric motility.
文摘Objective To observe the clinical effect of wristankle acupuncture on postpartum abdominal pain and its influence on serum beta-endorphin (β-EP) level in puerpera.Methods Seventy patients with postpartum abdominal pain were randomly divided into an acupuncture+herbal medication group (35 cases,1 case dropped out) and a herbal medication group(35 cases,2cases dropped out).In the herbal medication group.
文摘Objective To investigate the feasibility and safety of single peroral choledochoscopy combined with radiofrequency ablation (RFA) for nonresectable extrahepatic cholangiocarcinoma (EHCC).Methods A total of 90 patients with suspected EHCC treated in Hangzhou First People's Hospital from January 2013 to January 2022 were reviewed.According to the diagnosis and treatment process,69 patients were finally included and divided into two groups.
文摘Objective To investigate the clinical characteristics of Chinese and Western medicine in patients with severe systemic lupus erythematosus(SSLE).Methods Clinical data of SSLE inpatients in Guangdong Provincial Hospital of Chinese Medicine from January 2011 to December 2017 were collected.The gender,age,clinical characteristics,organ damage,Chinese medicine syndrome,tongue and pulse manifestation,mortality and cause of death were statistically analyzed by retrospective study.
文摘Objective To observe the clinical efficacy of acupuncture of revised acupoint combination around theskull base in treating post-stroke mild cognitive impairment(PSMCI),and preliminary explore its action mechanism.Methods A total of 76 PSMCI patients were randomly divided into an observation group(38 cases,4cases dropped off)and a control group(38 cases,3cases dropped off,1 case was removed).
文摘Objective To investigate the inhibitory effect of Gandou Decoction combined with penicillamine on cuproptosis in the liver of toxic milk (TX) mice,which serve as a model for Wilson's disease (WD).Methods Using dilute lethal (DL) mice as the normal control group,and TX mice were randomly divided into model group,Gandou Decoction group,penicillamine group,and Gandou Decoction combined with penicillamine group,with 8 mice in each group.
