Field training is the backbone of the teacher-preparation process.Its importance stems from the goals that colleges of education aim to achieve,which include bridging the gap between theory and practice and aligning w...Field training is the backbone of the teacher-preparation process.Its importance stems from the goals that colleges of education aim to achieve,which include bridging the gap between theory and practice and aligning with contemporary educational trends during teacher training.Currently,trainee students attendance in field training is recordedmanually through signatures on attendance sheets.However,thismethod is prone to impersonation,time wastage,and misplacement.Additionally,traditional methods of evaluating trainee students are often susceptible to human errors during the evaluation and scoring processes.Field training also lacks modern technology that the supervisor can use in case of his absence from school to monitor the trainee students’implementation of the required activities and tasks.These shortcomings do not meet the needs of the digital era that universities are currently experiencing.As a result,this paper presents a smart management system for field training based on Internet of Things(IoT)and mobile technology.It includes three subsystems:attendance,monitoring,and evaluation.The attendance subsystem uses an R307 fingerprint sensor to record trainee students’attendance.The Arduino Nano microcontroller transmits attendance data to the proposed Android application via an ESP-12F Wi-Fi module,which then forwards it to the Firebase database for storage.The monitoring subsystem utilizes Global Positioning System(GPS)technology to continually track trainee students’locations,ensuring they remain at the school during training.It also enables remote communication between trainee students and supervisors via audio,video,or text by integrating video call and chat technologies.The evaluation subsystem is based on three items:an online exam,attendance,and implementation of required activities and tasks.Experimental results have demonstrated the accuracy and efficiency of the proposed management system in recording attendance,as well as in monitoring and evaluating trainee students during field traiing.展开更多
Lithium-mediated nitrogen reduction reaction(LMNRR)is a promising route for sustainable ammonia synthesis,but the generation of excessive solid electrolyte interphase(SEI)severely limits its efficiency.Here,we tackle ...Lithium-mediated nitrogen reduction reaction(LMNRR)is a promising route for sustainable ammonia synthesis,but the generation of excessive solid electrolyte interphase(SEI)severely limits its efficiency.Here,we tackle this challenge by introducing n-hexane as an electrolyte additive to weaken LiClO4 ionization,achieving minimized dissociation via squeezed solvation shells with compact ion pairs.Molecular dynamics simulations and experimental characterizations reveal that n-hexane enriches anion coordination around Li+,endowing the electrolyte with robust anti-reduction capability.This suppresses SEI overgrowth,reduces interfacial resistance,and accelerates N2 diffusion.Consequently,a thinner,inorganic-rich SEI is formed,enabling high nitrogen flux and rapid active Li3N generation kinetics.Consequently,the proof-of-concept system achieves unprecedentedly high Faradaic efficiency of 53.8%±8.2%at 10 mA cm^(−2)and NH_(3) yield rate of 88.57±9.5 nmol s^(−1)cm^(−2)under ambient conditions,making a giant step further toward industrializing the electrochemical ammonia production.展开更多
Background:In mental health,recovery is emphasized,and qualitative analyses of service users’narratives have accumulated;however,while qualitative approaches excel at capturing rich context and generating new concept...Background:In mental health,recovery is emphasized,and qualitative analyses of service users’narratives have accumulated;however,while qualitative approaches excel at capturing rich context and generating new concepts,they are limited in generalizability and feasible data volume.This study aimed to quantify the subjective life history narratives of users of psychiatric home-visit nursing using natural language processing(NLP)and to clarify the relationships between linguistic features and recovery-related indicators.Methods:We conducted audio-recorded and transcribed semi-structured interviews on daily life verbatim and collected self-report questionnaires(Recovery Assessment Scale[RAS])and clinician ratings(Global Assessment of Functioning[GAF])from Japanese users of psychiatric home-visit nursing.Using the artificial intelligence-based topic-modeling method BERTopic,we extracted topics from the interview texts and calculated each participant’s topic proportions,and then examined associations between topic proportions and recovery-related indicators using Pearson correlation analyses.Results:“School”showed a significant positive correlation with RAS(r=0.39,p=0.05),whereas“Family”showed a significant negative correlation(r=–0.46,p=0.02).GAF was positively correlated with word count(r=0.44,p=0.02)and“Hospital”(r=0.42,p=0.03),and negatively correlated with“Backchannels”(aizuchi)(r=–0.41,p=0.03).Conclusion:The present results suggest that the quantity,quality,and content of narratives can serve as useful indicators of mental health and recovery,and that objective NLP-based analysis of service users’narratives can complement traditional self-report scales and clinician ratings to inform the design of recovery-oriented care in psychiatric home-visit nursing.展开更多
Hard carbon(HC)is a promising anode candidate for sodium-ion batteries(SIBs),yet its application is plagued by unstable interfaces and poor long-term cyclability.Herein,we develop a facile solvent evaporation strategy...Hard carbon(HC)is a promising anode candidate for sodium-ion batteries(SIBs),yet its application is plagued by unstable interfaces and poor long-term cyclability.Herein,we develop a facile solvent evaporation strategy to synthesize ultrathin Al_(2)O_(3)-coated biomass-derived HC(GSC-Al_(2)O_(3)-3%).The conformal Al_(2)O_(3)layer passivates defects and micropores,suppresses side reactions,and promotes the formation of a robust organic-inorganic hybrid solid electrolyte interphase.Comprehensive characterizations,including in situ X-ray diffraction,ex situ Raman spectra,X-ray photoelectron spectroscopy,time of flight secondary ion mass spectrometry,solid-state 27Al nuclear magnetic resonance,and atomic force microscope modulus mapping,demonstrate that Al_(2)O_(3)actively participates in SEI reconstruction,enhancing the chemical and mechanical stability.Electrochemical tests reveal that the optimized GSC-Al_(2)O_(3)-3%anode delivers 91%capacity retention after 1000 cycles at 1.0 A g^(-1),and possesses excellent wide-temperature tolerance(149.3 mAh g^(-1)at-30℃and 286.8 mAh g^(-1)at 60℃).Mechanistic studies confirm a synergistic Na+storage process involving"adsorption-intercalation-pore filling,"while density functional theory calculations and electrostatic potential mapping reveal that Al_(2)O_(3)coating regulates interfacial charge distribution and reduces Na+migration barriers.A full cell paired with a NaNi_(0.5)Fe_(0.5)MnO_(4)cathode exhibits a high initial capacity of 395.7 mAh g^(-1)and outstanding cycling stability(200 cycles).This work provides fundamental mechanistic insights into interfacial engineering of HC and establishes a cost-effective,scalable route for the next generation highperformance SIBs.展开更多
Conventional treatments for non-small cell lung cancer(NSCLC)suffer from low remission rates,high drug resistance,and severe adverse effects.To leverage the therapeutic potential of reactive oxygen species(ROS),nanoca...Conventional treatments for non-small cell lung cancer(NSCLC)suffer from low remission rates,high drug resistance,and severe adverse effects.To leverage the therapeutic potential of reactive oxygen species(ROS),nanocatalytic medicine utilizes nanomaterials to generate ROS specifically within tumor sites,enabling efficient and targeted cancer treatment.In this study,hyaluronic acid(HA)-modified copper-N,N-dimethyl-Nphenylsulfonylbisamine(DMSA)-assembled nanoparticles(Cu-DMSA-HA NPs)are developed with tumor-targeting capability and efficiently catalyze ROS production via coordination chemistry.Targeted delivery is facilitated by HA surface modification through recognition of overexpressed cluster of differentiation 44 receptors on cancer cells,which enhances nanoparticle uptake.Once internalized,intracellular glutathione is depleted by the NPs,followed by a Fenton-like reaction that sustains ROS production.Both in vitro and in vivo studies demonstrate that this catalytic strategy effectively inhibits DNA replication,prevents cell cycle progression,downregulates glutathione peroxidase 4 expression,induces ferroptosis,and ultimately suppresses NSCLC progression.Overall,the readily prepared Cu-DMSA-HA NPs exhibit robust catalytic activity and tumor specificity,highlighting their strong potential for clinical translation in nanocatalytic cancer therapy.展开更多
Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin...Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin monoclonal antibodies would be more useful for dissecting human allogenic immune responses, i. e. responses to histocompatibility and blood group antigens. Thirdly, they are of interest in capturing human immune responses such as antibodies produced in lymph nodes draining a tumor or autoimmune responses, and producing of human antibodies for possible therapeutic applications.展开更多
Lithium metal batteries(LMBs)are promising candidates for next-generation high-energy-density storage devices.However,an unstable lithium metal anode poses significant issues that critically compromise battery safety ...Lithium metal batteries(LMBs)are promising candidates for next-generation high-energy-density storage devices.However,an unstable lithium metal anode poses significant issues that critically compromise battery safety and cycle life,including lithium dendrite formation,solid electrolyte interphase degradation,dead lithium accumulation,and substantial volume fluctuations during cycling.These problems can be addressed by regulating lithium deposition and suppressing side reactions through the modification of copper current collectors using three classes of materials:metal and metal oxide,carbon,and polymer materials.This review comprehensively examines recent advances in the application of these materials as current collector coatings.Particularly,their distinct roles in the lithium deposition process are analyzed to understand how they mitigate the issues associated with the lithium metal anode.Furthermore,their inherent limitations are considered to inform future research directions.While each class of materials offers specific advantages,multifunctionality is required to effectively regulate lithium deposition.In prospect,a novel composite copper current collector design that integrates the merits of the aforementioned advanced materials is proposed.The insights from this review provide valuable guidance for the rational design of modified copper current collectors,which would significantly improve the safety and cycle life of LMBs and advance their commercialization.展开更多
Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor ...Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor group,a chemotherapy group,and an acupuncture group,and an EA group,with six mice in each one.Except in the sham-operation group,4T1 cells were injected into the fourth mammary fat pad on the right side of each mouse in the rest groups to develop breast-cancer bearing models.In the sham-operation group,the sham operation was underwent by injecting normal saline with the same volume as 4T1 cell suspension.After successful modeling,the intraperitoneal injection of doxorubicin solution was delivered in the chemotherapy group.In the acupuncture group,needles was inserted at“Zusanli”(ST36)and“Sanyinjiao”(SP6)perpendicularly,without manipulation,once daily and needles were re-tained for 15 min.In the EA group,on the basis of the operation as the acupuncture group,electric stimulation was attached to“ST36”(positive electrode)and“SP6”(negative electrode),once daily,15 min each time.Starting on Day 1 of intervention,the changes of tumor volume was observed once every two days in each group photographed.The sample was collected on Day 21 of intervention.The tumors were photographed and weighed.Hematoxylin-eosin(HE)staining was used to observe the pathologi-cal changes of tumor tissue.Western blot,fluorescence quantitative polymerase chain reaction(FQ-PCR),and immunohistochemistry(IHC)were adopted to detect the protein expression,mRNA expression,and positive expression of CYLD,BCL-3,and P50 in tumor tissue,respectively.Results:(1)The tumor volume in the tumor group,chemotherapy group,acupuncture group,and EA group was(815.67±275.61)mm^(3),(197.50±66.11)mm^(3),(412.62±58.96)mm^(3),and(327.51±125.46)mm^(3),respectively,and the tumor mass was(1.21±0.39)g,(0.39±0.05)g,(0.66±0.06)g,and(0.55±0.11)g on Day 21 of intervention,respectively.Compared with the tumor group,the tumor vol-ume and mass were lower in the chemotherapy group,acupuncture group,and EA group(P<0.05).(2)Respectively,in the tumor group,chemotherapy group,acupuncture group,and EA group,the mRNA expression of CYLD in tumor tissue was 1.01±0.19,2.90±0.24,2.55±0.03,and 4.01±0.32;the protein expression was 1.00±0.00,1.88±0.16,1.64±0.11,and 2.30±0.29;and the positive expres-sion was 0.06±0.01,0.11±0.01,0.11±0.004,and 0.14±0.003.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of CYLD were higher in the inter-vention groups(P<0.05),and each expression in the EA group was higher in comparison with the chemotherapy and acupuncture groups(P<0.05).(3)The mRNA expression of BCL-3 in tumor tissue was 3.34±0.27,1.11±0.04,1.25±0.10,and 1.00±0.04;and the protein expression was 1.00±0.00,0.59±0.15,0.38±0.08,and 0.38±0.13;and the positive expression was 0.12±0.01,0.06±0.02,0.07±0.01,and 0.05±0.003 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and pos-itive expression of BCL-3 were all lower in the intervention groups(P<0.05).(4)The mRNA expression of P50 in the tumor tissue was 3.69±0.17,1.69±0.07,1.74±0.05,and 1.00±0.06;and the pro-tein expression was 1.00±0.00,0.33±0.15,0.67±0.14,and 0.38±0.21;and the positive expression was 0.13±0.01,0.07±0.02,0.08±0.003,and 0.06±0.01 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of P50 in the tumor tissues of mice were lower in the inter-vention groups(P<0.05),and the mRNA expression in the EA group was lower when compared with the chemotherapy group and acupuncture group(P<0.05),while the protein expression of it in the chemotherapy group was lower than that in the acupuncture group(P<0.05).Conclusion:All of chemotherapy,acupuncture,and EA can inhibit tumor growth,which may be related to the up-regulation of CYLD expression and the down-regulation of BCL-3 and P50 expression in breast can-cer tissue.EA obtains a superior effect for up-regulating CYLD expression in comparison with chemother-apy and acupuncture.展开更多
Over recent decades,fuel cell technologies have emerged as viable solutions to address the energy and environmental challenges stemming from fossil fuel dependence.Especially,ammonia has gained increasing attention as...Over recent decades,fuel cell technologies have emerged as viable solutions to address the energy and environmental challenges stemming from fossil fuel dependence.Especially,ammonia has gained increasing attention as an attractive alternative to hydrogen,offering comparable energy density while maintaining carbon-free characteristics,along with superior storage and transport properties that give direct ammonia fuel cells(DAFCs)distinct safety advantages over hydrogen-based systems.Central to this technology is the anodic ammonia oxidation reaction(AOR),where platinum(Pt)remains the most efficient catalyst after years of intensive research.This review offers a comprehensive overview of Ptbased AOR electrocatalysts with potential for application in low-temperature DAFCs.Following an introductory section highlighting key historical developments and catalytic breakthroughs,a fundamental understanding of low-temperature DAFC operation and AOR mechanisms is systematically presented.Subsequently,it outlines the advancements in Pt-based catalysts from simple monometallic systems to sophisticated multimetallic alloys and composites,highlighting material innovations and performance enhancements.Afterward,key challenges and future research directions for advancing AOR electrocatalysts are identified,with the aim of providing valuable guidance for developing practical,highperformance,and low-temperature DAFC systems.展开更多
Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in t...Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated.The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions,thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.Methods:Sixty healthy male Wistar rats were randomly divided into four groups:Control,control+moxibustion,model,and moxibustion groups.A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG,with a dose of 1 mL/100 g body weight,once daily for 16 weeks.The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling,continuing for 16 weeks.After the experiment,gastric tissue was collected,and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining.Immunohistochemistry(IHC)and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues.Enzyme-linked immunosorbent assays(ELISA)were used to measure the levels of interleukin-12(IL-12),interferon-gamma(IFN-γ),and tumor necrosis factor-beta(TNF-β)in rat serum.Results:Upon macroscopic observation,the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red,with a glossy mucosal surface,normal gastric wall elasticity,and clear,regular mucosal folds,without hyperplasia or bleeding points.In the model group,the gastric mucosa was reduced in volume,the gastric wall thinned,elasticity decreased,mucosal folds were disordered,and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed.In the moxibustion group,the gastric mucosa showed decreased elasticity,with disordered mucosal folds and granular hyperplasia.After H&E staining,the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa,submucosa,and muscularis propria,without hyperplasia or keratinization.In the model group,the epithelial glands in the gastric mucosa were disordered,with varied cell morphologies,thickened submucosa,and disrupted squamous epithelium that invaded downward into the muscularis propria.In the moxibustion group,the squamous epithelium did not invade the muscularis propria.IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group(P<0.05)and lower expression levels in the model group than in the control group(P<0.05).The moxibustion group showed higher CD80 and CD86 levels than those in the model group(P<0.05).Western blotting indicated that CD80 and CD86 levels were higher in the moxibustion group than in the model group(P<0.05).ELISA results showed higher IL-12 levels in the model group than in the control group(P<0.05)and higher TNF-βand IFN-γlevels in the moxibustion group than in the model group(P<0.01).Conclusion:Direct moxibustion alleviates the pathological progression of gastric cancer in an MNNGinduced rat model.Its mechanisms may involve effects on the state of antigen-presenting cells,thereby promoting T cell activation and enhancing immune function.展开更多
Conjugated microporous polymers(CMPs)have attracted considerable attention as potential organic anode materials for sodium-ion batteries(SIBs)due to their flexible chemical structure,high porosity,environmental friend...Conjugated microporous polymers(CMPs)have attracted considerable attention as potential organic anode materials for sodium-ion batteries(SIBs)due to their flexible chemical structure,high porosity,environmental friendliness,and cost effectiveness.However,the inherent shortcomings of organic electrodes,such as low conductivity,high solubility in electrolyte,narrow material utilization,etc.,limit their further development.In this work,we successfully prepared a novel porous polyimide PPD containing multicarbonyl active centers via the polycondensation of pyromellitic dianhydride(PMDA)and2,6-diaminoanthraquinone(DAAQ).The stable conjugated structure and multiple redox centers give the polymer high reversible specific capacity(244.6 m Ah/g after 100 cycles at 100 m A/g),ultra-long cycle stability(100.7 m Ah/g after 2000 cycles at 1.0 A/g),and predominant rate capability.Meanwhile,the sodium storage mechanism of the electrode materials during the charging and discharging process is investigated by ex-situ XPS/FTIR analysis.Due to the exceptional electrochemical properties and simple synthesis method,this work may shed light on the preparation of polyimide-based anodes for high specific capacity and rate capability secondary batteries.展开更多
Background:PANoptosis has been identified as a robust inflammatory cell death pathway triggered upon host defense against invaded pathogens such as bacteria and viruses,however,pathogen-free tumor PANoptosis has not b...Background:PANoptosis has been identified as a robust inflammatory cell death pathway triggered upon host defense against invaded pathogens such as bacteria and viruses,however,pathogen-free tumor PANoptosis has not been achieved yet.Reactive oxygen and nitrogen species capable of inducing robust and diverse cell death pathways such as pyroptosis,apoptosis,and necroptosis are supposed to be the potential triggers for tumor PANoptosis by ultrasound(US)-controlled sono-piezodynamic therapy.Methods:S-nitrosothiols(SNO)-zinc peroxide(ZnO_(2))@cyclic dinucleotide(CDN)@mesoporous tetragonal barium titanate(mtBTO)nanoparticles(NZCB NPs)were synthesized by hydrothermal method with subsequent annealing,in situ growth,and finally surface functionalization.Scanning electron microscopy,transmission electron microscopy,X-ray diffraction,atomic force microscopy,Fourier transform infrared spectroscopy,and electron spin resonance were used for materials characterizations.Murine melanoma B16 cells are employed to investigate the in vitro US-initiated tumor PANoptosis by NZCB NPs.In vivo US-initiated tumor PANoptosis was investigated on B16 tumor-bearing C57BL/6J mice.Results:A“boiling-bubbling”strategy is developed to endow the piezoelectric BTO nanocatalysts,with mesoporous architecture,which enables the encapsulation of the immune-agonist CDN(9.4 wt%)to initiate innate immunity of the host.Then,SNO-functionalized ZnO_(2)was further employed to cap the mesoporous nanocatalysts,forming multifunctional piezocatalytic NZCB NPs.Under US irradiation,intracellular massive reactive oxygen and nitrogen species such as superoxide anion radicals,nitric oxide(NO),and peroxynitrite(ONOO-)could be produced from the piezoelectric NZCB NPs,which,synergized with CDN-triggered anti-tumoral immunity,lead to highly immunogenic tumor PANoptosis by NZCB NPs through the tumor microenvironment remodeling.Intratumoral injection of NZCB NPs leads to substantial tumor PANoptosis with immune potentiation,ultimately destroying the tumor xenografts effectively.Conclusion:The present work presents the mesostructure design of piezocatalytic nanomaterials and the crosstalk between oxidative stress and anti-tumor immunity within the tumor,facilitating promising tumor PANoptosis by nanocatalytic oxidation with high effectiveness and biocompatibility.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is a malignant disease with high incidence and mortality worldwide.This study focuses on the TP53 target protein to investigate the potential therapeutic effect of tetrahydrocur...BACKGROUND Hepatocellular carcinoma(HCC)is a malignant disease with high incidence and mortality worldwide.This study focuses on the TP53 target protein to investigate the potential therapeutic effect of tetrahydrocurcumin(THC)on HCC and its mechanism of action.The research hypothesis is that THC can inhibit the proliferation,migration,and invasion of HCC cells,and promote their apoptosis by regulating the TP53 target protein.AIM To explore the mechanism by which THC inhibits HCC cell proliferation via the TP53 signaling pathway.METHODS Potential targets of THC and HCC were identified from multiple databases.The core targets were subjected to analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases,and visualization processing,using the online platform Metascape to identify the key molecules and signaling pathways involved in the action of THC against HCC.The molecular mechanisms of action of THC against TP53 in the inhibition of HCC cells were verified using cell counting kit-8,Transwell,apoptosis,and western blotting assays.RESULTS Molecular docking results showed that THC had a high score for the TP53 target protein.In vitro experiments indicated that THC effectively inhibited the proliferation and migration of HCC cells,and affected the expression levels of TP53,MDM2,cyclin B,Bax,Bcl-2,caspase-9,and caspase-3.CONCLUSION THC induces the apoptosis of HCC cells through the TP53 signaling pathway,thereby inhibiting their proliferation and migration.展开更多
BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex educat...BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex education started relatively late,yet there are many problems in the development of sex education in China;for example,college students lack knowledge of sexuality.AIM To explore the perception of sex education among medical college students.METHODS Students majoring in medicine in a medical school were selected as the survey subjects.Anonymous online questionnaires were used to conduct the survey,and the results were analyzed using GraphPad Prism,SPSS,Microsoft Excel,and other software.The questionnaire was administered to understand the source of sexual knowledge,sexual responsibility,mastery of sexual knowledge,and distress caused by sexual problems.RESULTS Most students majoring in medicine had no formal sex education,lacked sexual knowledge,or had a biased understanding of sexual responsibility.This study analyzed future research trends in sex education based on relevant achievements in the Chinese context and abroad to further realize the practical significance and value of sex education popularization in China and provide recommendations for parents and schools at different levels.CONCLUSION Sex education should be conducted among college students,and medical colleges and universities should strengthen scientific sex education.展开更多
BACKGROUND Liver cancer has a high incidence and mortality worldwide,especially in China.Herein,we investigated the therapeutic effect and mechanism of tetrahydrocurcumin against hepatocellular carcinoma(HCC),with a f...BACKGROUND Liver cancer has a high incidence and mortality worldwide,especially in China.Herein,we investigated the therapeutic effect and mechanism of tetrahydrocurcumin against hepatocellular carcinoma(HCC),with a focus on the of phosphoinositide 3-kinases(PI3K)/AKT signaling pathway.AIM To investigate the effects and mechanism of tetrahydrocurcumin in HCC cell lines HepG2 and Huh7.METHODS Using Metascape,we analyzed the potential targets of tetrahydrocurcumin in HCC.Molecular docking validation was performed using SYBYL2.0.Cell Counting Kit-8,wound healing,and transwell assays were performed to evaluate the effects of tetrahydrocurcumin on HepG2 and Huh7 cell migration,invasion,and apoptosis.The expression of PI3K/AKT signaling pathway-related proteins was detected by western blotting.RESULTS Network pharmacology and molecular docking showed that tetrahydrocurcumin has high binding affinity for phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha.In vitro experiments demonstrated that tetrahydrocurcumin suppressed the migration and invasion of liver cancer cells,promoted their apoptosis,and downregulated the expression of p-PI3K,p-AKT,and B cell leukemia/lymphoma 2,while upregulating caspase-3,p53,and B cell leukemia/lymphoma 2 associated X.CONCLUSION In summary,tetrahydrocurcumin suppresses PI3K/AKT signaling,promotes apoptosis,and prevents the migration and invasion of liver cancer cells.展开更多
Objective: To investigate the reliability for fast estimation of Michaelis-Menten constant (Km) with calibrated specific activity at only two medium concentrations of substrate by both simulation and experimentation w...Objective: To investigate the reliability for fast estimation of Michaelis-Menten constant (Km) with calibrated specific activity at only two medium concentrations of substrate by both simulation and experimentation with arylesterase (ArE)as model. Methods: Initial rates were simulated by randomly inserting uniform absolute error, and the experimental initial rates of ArE were determined by measuring the increaser of product absorbance. Calibrated specific activities at two substrate concentrations were obtained by regression analysis, and Km was calculated according to Michaelis-Menten equation. Results: By simulation with calibrated specific activities at two medium substrate concentrations, Km could be calculated according to Michaelis-Menten equation with reasonable precision and accuracy. By experimentation with substrates of 2-naphthyl acetate, phenyl acetate, and p-nitrophenyl acetate, there were no differences between the mean and SD of Km of ArE for either substrate by this linear kinetic method and the Lineweaver-Burk plot. Conclusion: This linear kinetic method was reliable for fast estimation of the Km of some specified enzyme on its substrate of lower solubility or lower sensitivity for quantification by common methods.展开更多
The tensile properties of a series of soybean protein yarns are tested in USTER THINKPAID Ⅲ. A nonlinear viscoelastic model has been proposed to describe the tensile behavior of soybean protein yarns. The model is co...The tensile properties of a series of soybean protein yarns are tested in USTER THINKPAID Ⅲ. A nonlinear viscoelastic model has been proposed to describe the tensile behavior of soybean protein yarns. The model is composed of a Maxwell element, a linear spring and a nonlinear spring. The tensile properties of soybean protein yam are analyzed. The stress-strain curves of the yams are fitted. The average breaking tenacity and specific work of rupture are calculated using the average breaking strain. Comparisons indicate that theoretical predictions conform the experimental results very well.展开更多
文摘Field training is the backbone of the teacher-preparation process.Its importance stems from the goals that colleges of education aim to achieve,which include bridging the gap between theory and practice and aligning with contemporary educational trends during teacher training.Currently,trainee students attendance in field training is recordedmanually through signatures on attendance sheets.However,thismethod is prone to impersonation,time wastage,and misplacement.Additionally,traditional methods of evaluating trainee students are often susceptible to human errors during the evaluation and scoring processes.Field training also lacks modern technology that the supervisor can use in case of his absence from school to monitor the trainee students’implementation of the required activities and tasks.These shortcomings do not meet the needs of the digital era that universities are currently experiencing.As a result,this paper presents a smart management system for field training based on Internet of Things(IoT)and mobile technology.It includes three subsystems:attendance,monitoring,and evaluation.The attendance subsystem uses an R307 fingerprint sensor to record trainee students’attendance.The Arduino Nano microcontroller transmits attendance data to the proposed Android application via an ESP-12F Wi-Fi module,which then forwards it to the Firebase database for storage.The monitoring subsystem utilizes Global Positioning System(GPS)technology to continually track trainee students’locations,ensuring they remain at the school during training.It also enables remote communication between trainee students and supervisors via audio,video,or text by integrating video call and chat technologies.The evaluation subsystem is based on three items:an online exam,attendance,and implementation of required activities and tasks.Experimental results have demonstrated the accuracy and efficiency of the proposed management system in recording attendance,as well as in monitoring and evaluating trainee students during field traiing.
基金supported by the National Natural Science Foundation of China (Grant No. U21A20332)support from the Collaborative Innovation Center of Suzhou Nano Science and Technology
文摘Lithium-mediated nitrogen reduction reaction(LMNRR)is a promising route for sustainable ammonia synthesis,but the generation of excessive solid electrolyte interphase(SEI)severely limits its efficiency.Here,we tackle this challenge by introducing n-hexane as an electrolyte additive to weaken LiClO4 ionization,achieving minimized dissociation via squeezed solvation shells with compact ion pairs.Molecular dynamics simulations and experimental characterizations reveal that n-hexane enriches anion coordination around Li+,endowing the electrolyte with robust anti-reduction capability.This suppresses SEI overgrowth,reduces interfacial resistance,and accelerates N2 diffusion.Consequently,a thinner,inorganic-rich SEI is formed,enabling high nitrogen flux and rapid active Li3N generation kinetics.Consequently,the proof-of-concept system achieves unprecedentedly high Faradaic efficiency of 53.8%±8.2%at 10 mA cm^(−2)and NH_(3) yield rate of 88.57±9.5 nmol s^(−1)cm^(−2)under ambient conditions,making a giant step further toward industrializing the electrochemical ammonia production.
文摘Background:In mental health,recovery is emphasized,and qualitative analyses of service users’narratives have accumulated;however,while qualitative approaches excel at capturing rich context and generating new concepts,they are limited in generalizability and feasible data volume.This study aimed to quantify the subjective life history narratives of users of psychiatric home-visit nursing using natural language processing(NLP)and to clarify the relationships between linguistic features and recovery-related indicators.Methods:We conducted audio-recorded and transcribed semi-structured interviews on daily life verbatim and collected self-report questionnaires(Recovery Assessment Scale[RAS])and clinician ratings(Global Assessment of Functioning[GAF])from Japanese users of psychiatric home-visit nursing.Using the artificial intelligence-based topic-modeling method BERTopic,we extracted topics from the interview texts and calculated each participant’s topic proportions,and then examined associations between topic proportions and recovery-related indicators using Pearson correlation analyses.Results:“School”showed a significant positive correlation with RAS(r=0.39,p=0.05),whereas“Family”showed a significant negative correlation(r=–0.46,p=0.02).GAF was positively correlated with word count(r=0.44,p=0.02)and“Hospital”(r=0.42,p=0.03),and negatively correlated with“Backchannels”(aizuchi)(r=–0.41,p=0.03).Conclusion:The present results suggest that the quantity,quality,and content of narratives can serve as useful indicators of mental health and recovery,and that objective NLP-based analysis of service users’narratives can complement traditional self-report scales and clinician ratings to inform the design of recovery-oriented care in psychiatric home-visit nursing.
基金supported by National Natural Science Foundation,China(Nos.52261135632,U21A20284)Natural Science Foundation of Henan,China(No.232300421080)+3 种基金Program for Innovative Team(in Science and Technology)in University of Henan Province,China(No.24IRTSTHN006)Henan Distinguished Foreign Scientists Workgroup in Electrochemical Energy Storage,China(No.GZS2015013)Key Scientific Research Programs in Universities of Henan Province,China-Special Projects for Basic Research(No.23ZX008)Cultivation Project of Tuoxin Team in Henan University of Technology,China(No.2024TXTD11)。
文摘Hard carbon(HC)is a promising anode candidate for sodium-ion batteries(SIBs),yet its application is plagued by unstable interfaces and poor long-term cyclability.Herein,we develop a facile solvent evaporation strategy to synthesize ultrathin Al_(2)O_(3)-coated biomass-derived HC(GSC-Al_(2)O_(3)-3%).The conformal Al_(2)O_(3)layer passivates defects and micropores,suppresses side reactions,and promotes the formation of a robust organic-inorganic hybrid solid electrolyte interphase.Comprehensive characterizations,including in situ X-ray diffraction,ex situ Raman spectra,X-ray photoelectron spectroscopy,time of flight secondary ion mass spectrometry,solid-state 27Al nuclear magnetic resonance,and atomic force microscope modulus mapping,demonstrate that Al_(2)O_(3)actively participates in SEI reconstruction,enhancing the chemical and mechanical stability.Electrochemical tests reveal that the optimized GSC-Al_(2)O_(3)-3%anode delivers 91%capacity retention after 1000 cycles at 1.0 A g^(-1),and possesses excellent wide-temperature tolerance(149.3 mAh g^(-1)at-30℃and 286.8 mAh g^(-1)at 60℃).Mechanistic studies confirm a synergistic Na+storage process involving"adsorption-intercalation-pore filling,"while density functional theory calculations and electrostatic potential mapping reveal that Al_(2)O_(3)coating regulates interfacial charge distribution and reduces Na+migration barriers.A full cell paired with a NaNi_(0.5)Fe_(0.5)MnO_(4)cathode exhibits a high initial capacity of 395.7 mAh g^(-1)and outstanding cycling stability(200 cycles).This work provides fundamental mechanistic insights into interfacial engineering of HC and establishes a cost-effective,scalable route for the next generation highperformance SIBs.
基金supported by National Natural Science Foundation of China (82272943)Shanghai Municipal Science and Technology Commission (21Y11913400)+1 种基金Fundamental Research Funds for the Central UniversitiesNational Key Research and Development Program of China (2022YFC2407405)
文摘Conventional treatments for non-small cell lung cancer(NSCLC)suffer from low remission rates,high drug resistance,and severe adverse effects.To leverage the therapeutic potential of reactive oxygen species(ROS),nanocatalytic medicine utilizes nanomaterials to generate ROS specifically within tumor sites,enabling efficient and targeted cancer treatment.In this study,hyaluronic acid(HA)-modified copper-N,N-dimethyl-Nphenylsulfonylbisamine(DMSA)-assembled nanoparticles(Cu-DMSA-HA NPs)are developed with tumor-targeting capability and efficiently catalyze ROS production via coordination chemistry.Targeted delivery is facilitated by HA surface modification through recognition of overexpressed cluster of differentiation 44 receptors on cancer cells,which enhances nanoparticle uptake.Once internalized,intracellular glutathione is depleted by the NPs,followed by a Fenton-like reaction that sustains ROS production.Both in vitro and in vivo studies demonstrate that this catalytic strategy effectively inhibits DNA replication,prevents cell cycle progression,downregulates glutathione peroxidase 4 expression,induces ferroptosis,and ultimately suppresses NSCLC progression.Overall,the readily prepared Cu-DMSA-HA NPs exhibit robust catalytic activity and tumor specificity,highlighting their strong potential for clinical translation in nanocatalytic cancer therapy.
文摘Ⅰ. INTRODUCTION There are essentially three reasons why human monoclonal antibodies are attractive to the investigator. The first is that the specificity of oxenogenic antibodies is often poor. Secondly, human origin monoclonal antibodies would be more useful for dissecting human allogenic immune responses, i. e. responses to histocompatibility and blood group antigens. Thirdly, they are of interest in capturing human immune responses such as antibodies produced in lymph nodes draining a tumor or autoimmune responses, and producing of human antibodies for possible therapeutic applications.
基金supported by the National Natural Science Foundation of China(grant numbers 52071225,22179143,and 22002176)the European Union’s Horizon Europe research and innovation program Electron Beam Emergent Additive Manufacturing(EBEAM)(grant number 101087143)+2 种基金a Norway Grant through the National Science Centre(project number 2019/34/H/ST8/00547)the National Key R&D Program of China(grant number 2021YFB3800300)the Jiangsu Funding Program for Excellent Postdoctoral Talent。
文摘Lithium metal batteries(LMBs)are promising candidates for next-generation high-energy-density storage devices.However,an unstable lithium metal anode poses significant issues that critically compromise battery safety and cycle life,including lithium dendrite formation,solid electrolyte interphase degradation,dead lithium accumulation,and substantial volume fluctuations during cycling.These problems can be addressed by regulating lithium deposition and suppressing side reactions through the modification of copper current collectors using three classes of materials:metal and metal oxide,carbon,and polymer materials.This review comprehensively examines recent advances in the application of these materials as current collector coatings.Particularly,their distinct roles in the lithium deposition process are analyzed to understand how they mitigate the issues associated with the lithium metal anode.Furthermore,their inherent limitations are considered to inform future research directions.While each class of materials offers specific advantages,multifunctionality is required to effectively regulate lithium deposition.In prospect,a novel composite copper current collector design that integrates the merits of the aforementioned advanced materials is proposed.The insights from this review provide valuable guidance for the rational design of modified copper current collectors,which would significantly improve the safety and cycle life of LMBs and advance their commercialization.
基金Supported by National Natural Science Foundation of China General Program:No.81072883,No.81173342,No.81473773Hebei Provincial Natural Science Foundation:No.2023423029。
文摘Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor group,a chemotherapy group,and an acupuncture group,and an EA group,with six mice in each one.Except in the sham-operation group,4T1 cells were injected into the fourth mammary fat pad on the right side of each mouse in the rest groups to develop breast-cancer bearing models.In the sham-operation group,the sham operation was underwent by injecting normal saline with the same volume as 4T1 cell suspension.After successful modeling,the intraperitoneal injection of doxorubicin solution was delivered in the chemotherapy group.In the acupuncture group,needles was inserted at“Zusanli”(ST36)and“Sanyinjiao”(SP6)perpendicularly,without manipulation,once daily and needles were re-tained for 15 min.In the EA group,on the basis of the operation as the acupuncture group,electric stimulation was attached to“ST36”(positive electrode)and“SP6”(negative electrode),once daily,15 min each time.Starting on Day 1 of intervention,the changes of tumor volume was observed once every two days in each group photographed.The sample was collected on Day 21 of intervention.The tumors were photographed and weighed.Hematoxylin-eosin(HE)staining was used to observe the pathologi-cal changes of tumor tissue.Western blot,fluorescence quantitative polymerase chain reaction(FQ-PCR),and immunohistochemistry(IHC)were adopted to detect the protein expression,mRNA expression,and positive expression of CYLD,BCL-3,and P50 in tumor tissue,respectively.Results:(1)The tumor volume in the tumor group,chemotherapy group,acupuncture group,and EA group was(815.67±275.61)mm^(3),(197.50±66.11)mm^(3),(412.62±58.96)mm^(3),and(327.51±125.46)mm^(3),respectively,and the tumor mass was(1.21±0.39)g,(0.39±0.05)g,(0.66±0.06)g,and(0.55±0.11)g on Day 21 of intervention,respectively.Compared with the tumor group,the tumor vol-ume and mass were lower in the chemotherapy group,acupuncture group,and EA group(P<0.05).(2)Respectively,in the tumor group,chemotherapy group,acupuncture group,and EA group,the mRNA expression of CYLD in tumor tissue was 1.01±0.19,2.90±0.24,2.55±0.03,and 4.01±0.32;the protein expression was 1.00±0.00,1.88±0.16,1.64±0.11,and 2.30±0.29;and the positive expres-sion was 0.06±0.01,0.11±0.01,0.11±0.004,and 0.14±0.003.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of CYLD were higher in the inter-vention groups(P<0.05),and each expression in the EA group was higher in comparison with the chemotherapy and acupuncture groups(P<0.05).(3)The mRNA expression of BCL-3 in tumor tissue was 3.34±0.27,1.11±0.04,1.25±0.10,and 1.00±0.04;and the protein expression was 1.00±0.00,0.59±0.15,0.38±0.08,and 0.38±0.13;and the positive expression was 0.12±0.01,0.06±0.02,0.07±0.01,and 0.05±0.003 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and pos-itive expression of BCL-3 were all lower in the intervention groups(P<0.05).(4)The mRNA expression of P50 in the tumor tissue was 3.69±0.17,1.69±0.07,1.74±0.05,and 1.00±0.06;and the pro-tein expression was 1.00±0.00,0.33±0.15,0.67±0.14,and 0.38±0.21;and the positive expression was 0.13±0.01,0.07±0.02,0.08±0.003,and 0.06±0.01 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of P50 in the tumor tissues of mice were lower in the inter-vention groups(P<0.05),and the mRNA expression in the EA group was lower when compared with the chemotherapy group and acupuncture group(P<0.05),while the protein expression of it in the chemotherapy group was lower than that in the acupuncture group(P<0.05).Conclusion:All of chemotherapy,acupuncture,and EA can inhibit tumor growth,which may be related to the up-regulation of CYLD expression and the down-regulation of BCL-3 and P50 expression in breast can-cer tissue.EA obtains a superior effect for up-regulating CYLD expression in comparison with chemother-apy and acupuncture.
基金supported by the National Natural Science Foundation of China(No.52401284)the Natural Science Foundation of Jiangsu Province(No.BK20240957)+1 种基金the Key Research and Development Program of Nantong(No.GZ2024005)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX25_3677)。
文摘Over recent decades,fuel cell technologies have emerged as viable solutions to address the energy and environmental challenges stemming from fossil fuel dependence.Especially,ammonia has gained increasing attention as an attractive alternative to hydrogen,offering comparable energy density while maintaining carbon-free characteristics,along with superior storage and transport properties that give direct ammonia fuel cells(DAFCs)distinct safety advantages over hydrogen-based systems.Central to this technology is the anodic ammonia oxidation reaction(AOR),where platinum(Pt)remains the most efficient catalyst after years of intensive research.This review offers a comprehensive overview of Ptbased AOR electrocatalysts with potential for application in low-temperature DAFCs.Following an introductory section highlighting key historical developments and catalytic breakthroughs,a fundamental understanding of low-temperature DAFC operation and AOR mechanisms is systematically presented.Subsequently,it outlines the advancements in Pt-based catalysts from simple monometallic systems to sophisticated multimetallic alloys and composites,highlighting material innovations and performance enhancements.Afterward,key challenges and future research directions for advancing AOR electrocatalysts are identified,with the aim of providing valuable guidance for developing practical,highperformance,and low-temperature DAFC systems.
基金Supported by the National Natural Science Foundation of China:81173342Scientific Research Ability Improvement Projects of Hebei College of Traditional Chinese Medicine(now known as Hebei University of Chinese Medicine):KTZ2019012Scientific Research Project of Hebei Province Administration of Traditional Chinese Medicine:2022361.2021095。
文摘Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated.The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions,thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.Methods:Sixty healthy male Wistar rats were randomly divided into four groups:Control,control+moxibustion,model,and moxibustion groups.A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG,with a dose of 1 mL/100 g body weight,once daily for 16 weeks.The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling,continuing for 16 weeks.After the experiment,gastric tissue was collected,and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining.Immunohistochemistry(IHC)and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues.Enzyme-linked immunosorbent assays(ELISA)were used to measure the levels of interleukin-12(IL-12),interferon-gamma(IFN-γ),and tumor necrosis factor-beta(TNF-β)in rat serum.Results:Upon macroscopic observation,the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red,with a glossy mucosal surface,normal gastric wall elasticity,and clear,regular mucosal folds,without hyperplasia or bleeding points.In the model group,the gastric mucosa was reduced in volume,the gastric wall thinned,elasticity decreased,mucosal folds were disordered,and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed.In the moxibustion group,the gastric mucosa showed decreased elasticity,with disordered mucosal folds and granular hyperplasia.After H&E staining,the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa,submucosa,and muscularis propria,without hyperplasia or keratinization.In the model group,the epithelial glands in the gastric mucosa were disordered,with varied cell morphologies,thickened submucosa,and disrupted squamous epithelium that invaded downward into the muscularis propria.In the moxibustion group,the squamous epithelium did not invade the muscularis propria.IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group(P<0.05)and lower expression levels in the model group than in the control group(P<0.05).The moxibustion group showed higher CD80 and CD86 levels than those in the model group(P<0.05).Western blotting indicated that CD80 and CD86 levels were higher in the moxibustion group than in the model group(P<0.05).ELISA results showed higher IL-12 levels in the model group than in the control group(P<0.05)and higher TNF-βand IFN-γlevels in the moxibustion group than in the model group(P<0.01).Conclusion:Direct moxibustion alleviates the pathological progression of gastric cancer in an MNNGinduced rat model.Its mechanisms may involve effects on the state of antigen-presenting cells,thereby promoting T cell activation and enhancing immune function.
基金supported by National Natural Science Foundation,China(Nos.52071132,U21A20284 and 52261135632)Natural Science Foundation of Henan,China(Nos.232300421080,242300421035)+2 种基金Program for Innovative Team(in Science and Technology)in University of Henan Province,China(No.24IRTSTHN006)Key Scientific Research Programs in Universities of Henan Province,China–Special Projects for Basic Research(No.23ZX008)the Natural Science Foundation of Hunan Province,China(No.2023JJ50287)。
文摘Conjugated microporous polymers(CMPs)have attracted considerable attention as potential organic anode materials for sodium-ion batteries(SIBs)due to their flexible chemical structure,high porosity,environmental friendliness,and cost effectiveness.However,the inherent shortcomings of organic electrodes,such as low conductivity,high solubility in electrolyte,narrow material utilization,etc.,limit their further development.In this work,we successfully prepared a novel porous polyimide PPD containing multicarbonyl active centers via the polycondensation of pyromellitic dianhydride(PMDA)and2,6-diaminoanthraquinone(DAAQ).The stable conjugated structure and multiple redox centers give the polymer high reversible specific capacity(244.6 m Ah/g after 100 cycles at 100 m A/g),ultra-long cycle stability(100.7 m Ah/g after 2000 cycles at 1.0 A/g),and predominant rate capability.Meanwhile,the sodium storage mechanism of the electrode materials during the charging and discharging process is investigated by ex-situ XPS/FTIR analysis.Due to the exceptional electrochemical properties and simple synthesis method,this work may shed light on the preparation of polyimide-based anodes for high specific capacity and rate capability secondary batteries.
基金supported by the Shanghai Rising-Star Program(23QA1407600,23QA1409500)the National Natural Science Foundation of China(82272135,82372121)the National Natural Science Foundation of Shanghai(22ZR1448200).
文摘Background:PANoptosis has been identified as a robust inflammatory cell death pathway triggered upon host defense against invaded pathogens such as bacteria and viruses,however,pathogen-free tumor PANoptosis has not been achieved yet.Reactive oxygen and nitrogen species capable of inducing robust and diverse cell death pathways such as pyroptosis,apoptosis,and necroptosis are supposed to be the potential triggers for tumor PANoptosis by ultrasound(US)-controlled sono-piezodynamic therapy.Methods:S-nitrosothiols(SNO)-zinc peroxide(ZnO_(2))@cyclic dinucleotide(CDN)@mesoporous tetragonal barium titanate(mtBTO)nanoparticles(NZCB NPs)were synthesized by hydrothermal method with subsequent annealing,in situ growth,and finally surface functionalization.Scanning electron microscopy,transmission electron microscopy,X-ray diffraction,atomic force microscopy,Fourier transform infrared spectroscopy,and electron spin resonance were used for materials characterizations.Murine melanoma B16 cells are employed to investigate the in vitro US-initiated tumor PANoptosis by NZCB NPs.In vivo US-initiated tumor PANoptosis was investigated on B16 tumor-bearing C57BL/6J mice.Results:A“boiling-bubbling”strategy is developed to endow the piezoelectric BTO nanocatalysts,with mesoporous architecture,which enables the encapsulation of the immune-agonist CDN(9.4 wt%)to initiate innate immunity of the host.Then,SNO-functionalized ZnO_(2)was further employed to cap the mesoporous nanocatalysts,forming multifunctional piezocatalytic NZCB NPs.Under US irradiation,intracellular massive reactive oxygen and nitrogen species such as superoxide anion radicals,nitric oxide(NO),and peroxynitrite(ONOO-)could be produced from the piezoelectric NZCB NPs,which,synergized with CDN-triggered anti-tumoral immunity,lead to highly immunogenic tumor PANoptosis by NZCB NPs through the tumor microenvironment remodeling.Intratumoral injection of NZCB NPs leads to substantial tumor PANoptosis with immune potentiation,ultimately destroying the tumor xenografts effectively.Conclusion:The present work presents the mesostructure design of piezocatalytic nanomaterials and the crosstalk between oxidative stress and anti-tumor immunity within the tumor,facilitating promising tumor PANoptosis by nanocatalytic oxidation with high effectiveness and biocompatibility.
基金The Central Government Guides Local Science and Technology Development Fund,No.2023JH6/100100021Liaoning Province Education Department Foundation of China,No.JYTMS20231393 and No.LJ212410164032+2 种基金Scientific Research Project of the Liaoning Province Education Department,No.SYYX2019015Science and Technology Foundation of Shenyang Medical College,No.20171004the Science and Technology Innovation Fund for Master Students of Shenyang Medical College,No.Y20220509.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is a malignant disease with high incidence and mortality worldwide.This study focuses on the TP53 target protein to investigate the potential therapeutic effect of tetrahydrocurcumin(THC)on HCC and its mechanism of action.The research hypothesis is that THC can inhibit the proliferation,migration,and invasion of HCC cells,and promote their apoptosis by regulating the TP53 target protein.AIM To explore the mechanism by which THC inhibits HCC cell proliferation via the TP53 signaling pathway.METHODS Potential targets of THC and HCC were identified from multiple databases.The core targets were subjected to analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases,and visualization processing,using the online platform Metascape to identify the key molecules and signaling pathways involved in the action of THC against HCC.The molecular mechanisms of action of THC against TP53 in the inhibition of HCC cells were verified using cell counting kit-8,Transwell,apoptosis,and western blotting assays.RESULTS Molecular docking results showed that THC had a high score for the TP53 target protein.In vitro experiments indicated that THC effectively inhibited the proliferation and migration of HCC cells,and affected the expression levels of TP53,MDM2,cyclin B,Bax,Bcl-2,caspase-9,and caspase-3.CONCLUSION THC induces the apoptosis of HCC cells through the TP53 signaling pathway,thereby inhibiting their proliferation and migration.
基金Supported by Scientific Research Project of College Students in Liaoning Province,No.S202410164004.
文摘BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex education started relatively late,yet there are many problems in the development of sex education in China;for example,college students lack knowledge of sexuality.AIM To explore the perception of sex education among medical college students.METHODS Students majoring in medicine in a medical school were selected as the survey subjects.Anonymous online questionnaires were used to conduct the survey,and the results were analyzed using GraphPad Prism,SPSS,Microsoft Excel,and other software.The questionnaire was administered to understand the source of sexual knowledge,sexual responsibility,mastery of sexual knowledge,and distress caused by sexual problems.RESULTS Most students majoring in medicine had no formal sex education,lacked sexual knowledge,or had a biased understanding of sexual responsibility.This study analyzed future research trends in sex education based on relevant achievements in the Chinese context and abroad to further realize the practical significance and value of sex education popularization in China and provide recommendations for parents and schools at different levels.CONCLUSION Sex education should be conducted among college students,and medical colleges and universities should strengthen scientific sex education.
基金the Central Government Guidance Local Science and Technology Development Fund,No.2023JH6/100100021Liaoning Province Education Department Foundation of China,No.JYTMS20231393+2 种基金Scientific Research Project of Liaoning Province Education Department,No.SYYX2019015Science and Technology Fund of Shenyang Medical College,No.20171004,Shenyang Medical College Master’s Science and Technology Innovation Fund,No.Y20220509Liaoning Provincial Department of Education Scientific Research Project,No.LJ212410164032.
文摘BACKGROUND Liver cancer has a high incidence and mortality worldwide,especially in China.Herein,we investigated the therapeutic effect and mechanism of tetrahydrocurcumin against hepatocellular carcinoma(HCC),with a focus on the of phosphoinositide 3-kinases(PI3K)/AKT signaling pathway.AIM To investigate the effects and mechanism of tetrahydrocurcumin in HCC cell lines HepG2 and Huh7.METHODS Using Metascape,we analyzed the potential targets of tetrahydrocurcumin in HCC.Molecular docking validation was performed using SYBYL2.0.Cell Counting Kit-8,wound healing,and transwell assays were performed to evaluate the effects of tetrahydrocurcumin on HepG2 and Huh7 cell migration,invasion,and apoptosis.The expression of PI3K/AKT signaling pathway-related proteins was detected by western blotting.RESULTS Network pharmacology and molecular docking showed that tetrahydrocurcumin has high binding affinity for phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha.In vitro experiments demonstrated that tetrahydrocurcumin suppressed the migration and invasion of liver cancer cells,promoted their apoptosis,and downregulated the expression of p-PI3K,p-AKT,and B cell leukemia/lymphoma 2,while upregulating caspase-3,p53,and B cell leukemia/lymphoma 2 associated X.CONCLUSION In summary,tetrahydrocurcumin suppresses PI3K/AKT signaling,promotes apoptosis,and prevents the migration and invasion of liver cancer cells.
文摘Objective: To investigate the reliability for fast estimation of Michaelis-Menten constant (Km) with calibrated specific activity at only two medium concentrations of substrate by both simulation and experimentation with arylesterase (ArE)as model. Methods: Initial rates were simulated by randomly inserting uniform absolute error, and the experimental initial rates of ArE were determined by measuring the increaser of product absorbance. Calibrated specific activities at two substrate concentrations were obtained by regression analysis, and Km was calculated according to Michaelis-Menten equation. Results: By simulation with calibrated specific activities at two medium substrate concentrations, Km could be calculated according to Michaelis-Menten equation with reasonable precision and accuracy. By experimentation with substrates of 2-naphthyl acetate, phenyl acetate, and p-nitrophenyl acetate, there were no differences between the mean and SD of Km of ArE for either substrate by this linear kinetic method and the Lineweaver-Burk plot. Conclusion: This linear kinetic method was reliable for fast estimation of the Km of some specified enzyme on its substrate of lower solubility or lower sensitivity for quantification by common methods.
文摘The tensile properties of a series of soybean protein yarns are tested in USTER THINKPAID Ⅲ. A nonlinear viscoelastic model has been proposed to describe the tensile behavior of soybean protein yarns. The model is composed of a Maxwell element, a linear spring and a nonlinear spring. The tensile properties of soybean protein yam are analyzed. The stress-strain curves of the yams are fitted. The average breaking tenacity and specific work of rupture are calculated using the average breaking strain. Comparisons indicate that theoretical predictions conform the experimental results very well.
