AIM:To identify the aetiology of open globe injuries at Hospital Universiti Sains Malaysia over a period of 10y and the prognostic factors for visual outcome.METHODS:Retrospective review of medical records of open glo...AIM:To identify the aetiology of open globe injuries at Hospital Universiti Sains Malaysia over a period of 10y and the prognostic factors for visual outcome.METHODS:Retrospective review of medical records of open globe injury cases that presented from January2000 to December 2009.Classification of open globe injury was based on the Birmingham Eye Trauma Terminology(BETT).Records were obtained with hospital permission via the in-house electronic patient management system,and the case notes of all patients with a diagnosis of open globe injury were scrutinised.Patients with prior ocular trauma,pre-existing ocular conditions affecting the visual acuity,contrast sensitivity,central vision or corneal thickness,as well as those with a history of previous intraocular or refractive surgery were excluded.Analysis of data was with SPSS version20.0.Ordinal logistic regression analysis was used to examine the association between prognostic factors and visual outcome.RESULTS:This study involved 220 patients(n=222eyes).The most common place of injury was the home(51.8%),followed by the workplace(23.4%).Among children aged less than 16y of age,domestic-related injury was the predominant cause(54.6%),while in those aged 16y and above,occupational injuries were the most common cause(40.0%).Most eyes(76.5%)had an initial visual acuity worse than 3/60,and in half of these,the visual acuity improved.The visual outcome was found to be significantly associated with the initial visual acuity(P【0.005),posterior extent of wound(P【0.001),length of wound(P【0.001),presence of hyphaema(P【0.001)and presence of vitreous prolapse(P【0.005).CONCLUSION:The most common causes of open globe injury are domestic accidents and occupational injuries.Significant prognostic factors for final visual outcome in patients with open globe injury are initial visual acuity,posterior extent and length of wound,presence of hyphaema and presence of vitreous prolapse.Awareness of the factors predicting a poor visual outcome may be helpful during counselling of patients with open globe injuries.展开更多
目的分析壮医经筋疗法联合桂枝加黄芪汤加减对肩周炎风寒湿痹证患者表面肌电信号及预后的影响。方法纳入2022年1月—2022年12月收治的114例肩周炎风寒湿痹证患者,使用简单随机法分别纳入联合组和对照组各57例。两组均接受壮医经筋疗法治...目的分析壮医经筋疗法联合桂枝加黄芪汤加减对肩周炎风寒湿痹证患者表面肌电信号及预后的影响。方法纳入2022年1月—2022年12月收治的114例肩周炎风寒湿痹证患者,使用简单随机法分别纳入联合组和对照组各57例。两组均接受壮医经筋疗法治疗,联合组加用桂枝加黄芪汤加减治疗,两组治疗均持续1个月。检测两组患者治疗前后肩关节功能评分、三角肌表面肌电信号等指标变化,评估两组预后。结果两组治疗后CMS量表疼痛、肌力、肩关节活动、功能活动评分均较治疗前升高;联合组治疗后Constant-Murley肩关节功能评分(Constant-Murley shoulder function score,CMS)量表疼痛、肌力、肩关节活动、功能活动评分均高于对照组,差异有统计学意义(P<0.05)。两组治疗后患侧三角肌表面积分肌电值(Integrated electromyography,iEMG)均较治疗前升高,疼痛视觉模拟评分(Visual analog score,VAS)评分均较治疗前下降;联合组治疗后患侧三角肌表面iEMG均高于对照组,其VAS评分低于对照组,差异有统计学意义(P<0.05)。联合组治疗后临床总有效率为97.40%(75/77),较对照组的86.67%(65/75)显著较高,差异有统计学意义(P<0.05)。结论在壮医经筋疗法的基础上联合桂枝加黄芪汤加减治疗,能够进一步改善肩周炎风寒湿痹证患者表面肌电信号、促进肩关节功能恢复及疼痛减轻,从而提高预后质量。展开更多
BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the para...BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the paradoxical role of C.albicans in CRC,aiming to determine whether it promotes or suppresses tumor development,with a focus on the mechanistic basis linked to its metabolic profile.AIM To investigate the dual role of C.albicans in the development and progression of CRC through metabolite profiling and to establish a prognostic model that integrates the microbial and metabolic interactions in CRC,providing insights into potential therapeutic strategies and clinical outcomes.METHODSA prognostic model integrating C. albicans with CRC was developed, incorporating enrichment analysis, immuneinfiltration profiling, survival analysis, Mendelian randomization, single-cell sequencing, and spatial transcriptomics.The effects of the C. albicans metabolite mixture on CRC cells were subsequently validated in vitro. Theprimary metabolite composition was characterized using liquid chromatography-mass spectrometry.RESULTSA prognostic model based on five specific mRNA markers, EHD4, LIME1, GADD45B, TIMP1, and FDFT1, wasestablished. The C. albicans metabolite mixture significantly reduced CRC cell viability. Post-treatment analysisrevealed a significant decrease in gene expression in HT29 cells, while the expression levels of TIMP1, EHD4, andGADD45B were significantly elevated in HCT116 cells. Conversely, LIME1 expression and that of other CRC celllines showed reductions. In normal colonic epithelial cells (NCM460), GADD45B, TIMP1, and FDFT1 expressionlevels were significantly increased, while LIME1 and EHD4 levels were markedly reduced. Following metabolitetreatment, the invasive and migratory capabilities of NCM460, HT29, and HCT116 cells were reduced. Quantitativeanalysis of extracellular ATP post-treatment showed a significant elevation (P < 0.01). The C. albicans metabolitemixture had no effect on reactive oxygen species accumulation in CRC cells but led to a reduction in mitochondrialmembrane potential, increased intracellular lipid peroxidation, and induced apoptosis. Metabolomic profilingrevealed significant alterations, with 516 metabolites upregulated and 531 downregulated.CONCLUSIONThis study introduced a novel prognostic model for CRC risk assessment. The findings suggested that the C.albicans metabolite mixture exerted an inhibitory effect on CRC initiation.展开更多
文摘AIM:To identify the aetiology of open globe injuries at Hospital Universiti Sains Malaysia over a period of 10y and the prognostic factors for visual outcome.METHODS:Retrospective review of medical records of open globe injury cases that presented from January2000 to December 2009.Classification of open globe injury was based on the Birmingham Eye Trauma Terminology(BETT).Records were obtained with hospital permission via the in-house electronic patient management system,and the case notes of all patients with a diagnosis of open globe injury were scrutinised.Patients with prior ocular trauma,pre-existing ocular conditions affecting the visual acuity,contrast sensitivity,central vision or corneal thickness,as well as those with a history of previous intraocular or refractive surgery were excluded.Analysis of data was with SPSS version20.0.Ordinal logistic regression analysis was used to examine the association between prognostic factors and visual outcome.RESULTS:This study involved 220 patients(n=222eyes).The most common place of injury was the home(51.8%),followed by the workplace(23.4%).Among children aged less than 16y of age,domestic-related injury was the predominant cause(54.6%),while in those aged 16y and above,occupational injuries were the most common cause(40.0%).Most eyes(76.5%)had an initial visual acuity worse than 3/60,and in half of these,the visual acuity improved.The visual outcome was found to be significantly associated with the initial visual acuity(P【0.005),posterior extent of wound(P【0.001),length of wound(P【0.001),presence of hyphaema(P【0.001)and presence of vitreous prolapse(P【0.005).CONCLUSION:The most common causes of open globe injury are domestic accidents and occupational injuries.Significant prognostic factors for final visual outcome in patients with open globe injury are initial visual acuity,posterior extent and length of wound,presence of hyphaema and presence of vitreous prolapse.Awareness of the factors predicting a poor visual outcome may be helpful during counselling of patients with open globe injuries.
文摘目的分析壮医经筋疗法联合桂枝加黄芪汤加减对肩周炎风寒湿痹证患者表面肌电信号及预后的影响。方法纳入2022年1月—2022年12月收治的114例肩周炎风寒湿痹证患者,使用简单随机法分别纳入联合组和对照组各57例。两组均接受壮医经筋疗法治疗,联合组加用桂枝加黄芪汤加减治疗,两组治疗均持续1个月。检测两组患者治疗前后肩关节功能评分、三角肌表面肌电信号等指标变化,评估两组预后。结果两组治疗后CMS量表疼痛、肌力、肩关节活动、功能活动评分均较治疗前升高;联合组治疗后Constant-Murley肩关节功能评分(Constant-Murley shoulder function score,CMS)量表疼痛、肌力、肩关节活动、功能活动评分均高于对照组,差异有统计学意义(P<0.05)。两组治疗后患侧三角肌表面积分肌电值(Integrated electromyography,iEMG)均较治疗前升高,疼痛视觉模拟评分(Visual analog score,VAS)评分均较治疗前下降;联合组治疗后患侧三角肌表面iEMG均高于对照组,其VAS评分低于对照组,差异有统计学意义(P<0.05)。联合组治疗后临床总有效率为97.40%(75/77),较对照组的86.67%(65/75)显著较高,差异有统计学意义(P<0.05)。结论在壮医经筋疗法的基础上联合桂枝加黄芪汤加减治疗,能够进一步改善肩周炎风寒湿痹证患者表面肌电信号、促进肩关节功能恢复及疼痛减轻,从而提高预后质量。
基金Supported by Gansu Province Joint Fund General Program,No.24JRRA878Gansu Provincial Science and Technology Program Project,No.24JRRA1020+2 种基金Gansu Province Key Talent Program,No.2025RCXM006Teaching Research and Reform Program for Postgraduate Education at Gansu University of Traditional Chinese Medicine(GUSTCM),No.YBXM-202406Special Fund for Mentors of“Qihuang Talents”in the First-Level Discipline of Chinese Medicine,No.ZYXKBD-202415。
文摘BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the paradoxical role of C.albicans in CRC,aiming to determine whether it promotes or suppresses tumor development,with a focus on the mechanistic basis linked to its metabolic profile.AIM To investigate the dual role of C.albicans in the development and progression of CRC through metabolite profiling and to establish a prognostic model that integrates the microbial and metabolic interactions in CRC,providing insights into potential therapeutic strategies and clinical outcomes.METHODSA prognostic model integrating C. albicans with CRC was developed, incorporating enrichment analysis, immuneinfiltration profiling, survival analysis, Mendelian randomization, single-cell sequencing, and spatial transcriptomics.The effects of the C. albicans metabolite mixture on CRC cells were subsequently validated in vitro. Theprimary metabolite composition was characterized using liquid chromatography-mass spectrometry.RESULTSA prognostic model based on five specific mRNA markers, EHD4, LIME1, GADD45B, TIMP1, and FDFT1, wasestablished. The C. albicans metabolite mixture significantly reduced CRC cell viability. Post-treatment analysisrevealed a significant decrease in gene expression in HT29 cells, while the expression levels of TIMP1, EHD4, andGADD45B were significantly elevated in HCT116 cells. Conversely, LIME1 expression and that of other CRC celllines showed reductions. In normal colonic epithelial cells (NCM460), GADD45B, TIMP1, and FDFT1 expressionlevels were significantly increased, while LIME1 and EHD4 levels were markedly reduced. Following metabolitetreatment, the invasive and migratory capabilities of NCM460, HT29, and HCT116 cells were reduced. Quantitativeanalysis of extracellular ATP post-treatment showed a significant elevation (P < 0.01). The C. albicans metabolitemixture had no effect on reactive oxygen species accumulation in CRC cells but led to a reduction in mitochondrialmembrane potential, increased intracellular lipid peroxidation, and induced apoptosis. Metabolomic profilingrevealed significant alterations, with 516 metabolites upregulated and 531 downregulated.CONCLUSIONThis study introduced a novel prognostic model for CRC risk assessment. The findings suggested that the C.albicans metabolite mixture exerted an inhibitory effect on CRC initiation.
