Total P (TP), total participate P (PP), total dissolved P (TDP), molybdatereactive P (MRP) and dissolved organic P (DOP) were determined in waters from pipe-drains (at 65-cmdepth) from the Broadbalk Experiment at Roth...Total P (TP), total participate P (PP), total dissolved P (TDP), molybdatereactive P (MRP) and dissolved organic P (DOP) were determined in waters from pipe-drains (at 65-cmdepth) from the Broadbalk Experiment at Rothamsted Research, UK. Average TPand PP exceeded 1 mgL^(-1) in about half of the 12 plots receiving superphosphate for the 5 measurements taken betweenDecember 2000 and April 2001. Ranging between 33.8% and 87.3% of TP, PP was the largest P fractionin drainage waters, with DOP, ranging from 0.5% to 26.2% of TP, being the smallest fraction Meanproportions of PP, MRP and DOP in TP in drainage waters were 63.4%, 32.5% and 4.1%, respectively.These findings support previous findings that P losses from soil to drainage waters were muchlargerthan previously thought, and could therefore make a significant contribution to eutrophication.展开更多
Remobilisation of nitrate in plants, especially in vacuole of plant, is mostly related to the qua- lity of agricultural products and the high nitrogen use efficiency in plants. Ion-selective microelectrodes offer a n...Remobilisation of nitrate in plants, especially in vacuole of plant, is mostly related to the qua- lity of agricultural products and the high nitrogen use efficiency in plants. Ion-selective microelectrodes offer a non-destructive and non-interruptive method to measure NO 3 gradients and electric potential differences across both the plasma membrane and tonoplast. Thus, a double-barrelled microelectrode backfilled with a membrane sensor for NO 3 embedded in poly vinyl chloride (PVC) can record the NO 3 activity in cytoplasm and vacuole of a cell. This paper presented how to make this kind of microelectrode and how to do the intracellular measurements on intact plants. Our result showed that nitrate activity was about 2.7 mmol L 1 in cytoplasm while 70 mmol L 1 in vacuole, which implicated that vacuole was a pool of nitrate in plants.展开更多
Chemosensory proteins (CSPs) are identifiable by four spatially conserved Cys-teine residues in their primary structure or by two disulfide bridges in their tertiary structure according to the previously identified ol...Chemosensory proteins (CSPs) are identifiable by four spatially conserved Cys-teine residues in their primary structure or by two disulfide bridges in their tertiary structure according to the previously identified olfactory specific-D related proteins. A genomics- and bioinformatics-based approach is taken in the present study to identify the putative CSPs in the malaria-carrying mosquito, Anopheles gambiae. The results show that five out of the nine annotated candidates are the most possible Anopheles CSPs of A. gambiae. This study lays the foundation for further functional identification of Anopheles CSPs, though all of these candidates need additional experimental verification.展开更多
基金Project supported by the Rothamsted International, UK the Scientific Research Foundation for Returned Overseas Chinese Scholars, the State Education Ministry of China and the Natural Science Foundation of Shaanxi Province, China (No. 2002D09).
文摘Total P (TP), total participate P (PP), total dissolved P (TDP), molybdatereactive P (MRP) and dissolved organic P (DOP) were determined in waters from pipe-drains (at 65-cmdepth) from the Broadbalk Experiment at Rothamsted Research, UK. Average TPand PP exceeded 1 mgL^(-1) in about half of the 12 plots receiving superphosphate for the 5 measurements taken betweenDecember 2000 and April 2001. Ranging between 33.8% and 87.3% of TP, PP was the largest P fractionin drainage waters, with DOP, ranging from 0.5% to 26.2% of TP, being the smallest fraction Meanproportions of PP, MRP and DOP in TP in drainage waters were 63.4%, 32.5% and 4.1%, respectively.These findings support previous findings that P losses from soil to drainage waters were muchlargerthan previously thought, and could therefore make a significant contribution to eutrophication.
基金supported by the National Natural Science Foundation of China(30270790).
文摘Remobilisation of nitrate in plants, especially in vacuole of plant, is mostly related to the qua- lity of agricultural products and the high nitrogen use efficiency in plants. Ion-selective microelectrodes offer a non-destructive and non-interruptive method to measure NO 3 gradients and electric potential differences across both the plasma membrane and tonoplast. Thus, a double-barrelled microelectrode backfilled with a membrane sensor for NO 3 embedded in poly vinyl chloride (PVC) can record the NO 3 activity in cytoplasm and vacuole of a cell. This paper presented how to make this kind of microelectrode and how to do the intracellular measurements on intact plants. Our result showed that nitrate activity was about 2.7 mmol L 1 in cytoplasm while 70 mmol L 1 in vacuole, which implicated that vacuole was a pool of nitrate in plants.
文摘Chemosensory proteins (CSPs) are identifiable by four spatially conserved Cys-teine residues in their primary structure or by two disulfide bridges in their tertiary structure according to the previously identified olfactory specific-D related proteins. A genomics- and bioinformatics-based approach is taken in the present study to identify the putative CSPs in the malaria-carrying mosquito, Anopheles gambiae. The results show that five out of the nine annotated candidates are the most possible Anopheles CSPs of A. gambiae. This study lays the foundation for further functional identification of Anopheles CSPs, though all of these candidates need additional experimental verification.
