Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confi...Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.展开更多
Rice seedling blight,caused by various fungi,including Fusarium oxysporum,poses a severe threat to rice production.As awareness grows regarding the environmental and safety hazards associated with the application of f...Rice seedling blight,caused by various fungi,including Fusarium oxysporum,poses a severe threat to rice production.As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight,there has been a shift in focus towards biological control agents.In this study,we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of F.oxysporum in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus T208.Additionally,our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual use.Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients,which were negatively correlated with the biomass of F.oxysporum-inoculated rice seedlings.Furthermore,this mixture led to higher stochastic assembly(average|βNTI|<2)and reduced selection pressures on rice rhizosphere bacteria compared with individual strain applications.The mixture of the T40 and T208 strains also significantly increased the expression of defense-related genes.In conclusion,the mixture of the T40 and T208 strains effectively modulates microbial community structures,enhances microbial network stability,and boosts the resistance against rice seedling blight.Our study supports the development and utilization of biological resources for crop protection.展开更多
The ratooning system enhances agricultural efficiency by reducing secondary sowing and resource input while maintaining rice yield parity with double cropping.However,the prolonged growth duration of the rice ratoonin...The ratooning system enhances agricultural efficiency by reducing secondary sowing and resource input while maintaining rice yield parity with double cropping.However,the prolonged growth duration of the rice ratooning system extends the exposure window to Magnaporthe oryzae infection,thereby elevating the probability of disease incidence.展开更多
Rice sheath blight(RSB)is a major destructive disease impeding rice production.Identifying key germplasm resources with increased resistance remains a challenge.However,the mechanisms underlying disease resistance are...Rice sheath blight(RSB)is a major destructive disease impeding rice production.Identifying key germplasm resources with increased resistance remains a challenge.However,the mechanisms underlying disease resistance are not yet fully understood.Cytochrome P450 monooxygenases(CYP450s)serve biosynthesis and metabolic detoxification functions in plants,but there is limited information about their role in the response induced by RSB.This study demonstrated that CYT02 belongs to the CYP73A100 subfamily and is a typical member of the CYP450s.Overexpression(OE)in rice of the cytochrome P450 monooxygenase cyt02 conferred increased resistance to RSB and increased vegetative tillering.Cyt02 may increase RSB resistance by regulating plant hormone synthesis,regulate reactive oxygen species(ROS)by coordinating the activity of antioxidant enzymes,and initiate phytoalexin synthesis in response to fungal infection.These research findings have laid a foundation for a deeper understanding of the function of cyt02 and offered a potential target gene for breeding rice varieties resistant to sheath blight.展开更多
In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial ...In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial abundance and maintaining community structures.To date,only 16 rice viruses have been documented in rice-growing regions.These viruses pose serious threats to rice production and have traditionally been identified only from rice plants and insect vectors by isolation techniques.Advances in next-generation sequencing(NGS)have made it feasible to discover viruses on a global scale.Recently,numerous viruses have been identified in plants and invertebrates using NGS technologies.In this review,we discuss viral studies in rice plants,invertebrate species,and weeds in rice fields.Many novel viruses have been discovered in rice ecosystems through NGS technologies,with some also detected using metatranscriptomic and small RNA sequencing.These analyses greatly expand our understanding of viruses in rice fields and provide valuable insights for developing efficient strategies to manage insect pests and virus-mediated rice diseases.展开更多
In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tend...In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tendency for high seed shattering in domesticated crops,such as rice,not only reduces paddy yield but also complicates mechanized harvesting.展开更多
Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified...Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified the cytoplasmic ATP-citrate lyase(ACL)subunit A2 in rice(Oryza sativa L.),OsACL-A2(Os12g0566300),as a critical factor for iron uptake and transport.The osacl-a2 mutant exhibited reduced leaf iron levels,leading to iron deficiency-induced chlorosis,activated defense signaling,and eventual necrosis in mature leaves.Additionally,blast resistance was weakened in immature osacl-a2 leaves.Exogenous iron supplementation rescued these defects.The mutant displayed reduced ATP-citrate lyase activity but increased citric acid levels compared with its wild type(WT),suggesting that the osacl-a2 mutation impairs enzyme activity.Thus,OsACL-A2-mediated citrate lyase activity plays a vital role in promoting iron uptake and associated blast resistance in rice.展开更多
Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,...Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,Sub1 and Dro1(Δbp:10 Mb),as well as Sub1 and TPP7(Δbp:6 Mb)were identified to exhibit long-range linkage disequilibrium(LRLD).Meta-QTL analysis further revealed that Sub1 and TPP7 co-segregated for tolerance to submergence at the germination and seedling stages.Based on this,we hypothesized that LRLD might influence plant responses to consecutive stresses.To test this hypothesis,we developed a structured recombinant inbred line population from a cross between Bhalum 2 and Nagina 22,with alleles(Sub1 and TPP7)in linkage equilibrium.Mendelian randomization analysis validated that the parental alleles,rather than the recombinant alleles of Sub1 and TPP7,significantly influenced 13 out of 41 traits under consecutive stress conditions.Additionally,16 minor additive effect QTLs were detected between the genomic regions,spanning Sub1 and TPP7 for various traits.A single allele difference between these genomic regions enhanced crown root number,root dry weight,and specific root area by 11.45%,15.69%,and 33.15%,respectively,under flooded germination conditions.Candidate gene analysis identified WAK79 and MRLK59 as regulators of stress responses during flooded germination,recovery,and subsequent water deficit conditions.These findings highlight the critical role of parental allele combinations and genomic regions between Sub1 and TPP7 in regulating the stress responses under consecutive stresses.Favourable haplotypes derived from these alleles can be utilized to improve stress resilience in direct-seeded rice.展开更多
The leaf is a major organ for photosynthesis,and its shape plays an important role in plant development and yield determination in rice(Oryza sativa L.).In this study,an adaxial curled leaf mutant,termed curly leaf 1-...The leaf is a major organ for photosynthesis,and its shape plays an important role in plant development and yield determination in rice(Oryza sativa L.).In this study,an adaxial curled leaf mutant,termed curly leaf 1-1(cul1-1),was obtained by chemical mutagenesis.The leaf rolling index of the cul1-1 mutant was higher than that of the wild-type,which was caused by the abnormal development of bulliform cells(BCs).We cloned the CUL1 gene by map-based cloning.A nonsense mutation was present in the cul1-1 mutant,converting a tryptophan codon into a stop codon.The CUL1 gene encodes a chromodomain,helicase/ATPase and DNA-binding domain containing protein.Genes related to leaf rolling and BC development,such as ADL1,REL1 and ROC5,were activated by the cul1-1 mutation.The trimethylation of lysine 27 in histone 3(H3K27me3),but not H3K4me3,at the ADL1,REL1 and ROC5 loci,was reduced in the cul1-1 mutant.High-throughput mRNA sequencing indicated that the cul1-1 mutation caused genome-wide differential gene expression.The differentially expressed genes were classified into a few gene ontology terms and Kyoto encyclopedia of genes and genomes pathways.In the natural population,22 missense genomic variations in the CUL1 locus were identified,which composed of 7 haplotypes.A haplotype network was also built with haplotype II as the ancestor.The findings revealed that CUL1 is essential for normal leaf development and regulates this process by inhibiting the expression of genes involved in leaf rolling and BC development.展开更多
Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield...Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.展开更多
Rice seed germination marks the start of cultivation and influences subsequent seedling growth,and is affected by hormones and environmental factors.Ubiquitination plays a critical role in this process by regulating h...Rice seed germination marks the start of cultivation and influences subsequent seedling growth,and is affected by hormones and environmental factors.Ubiquitination plays a critical role in this process by regulating hormonal homeostasis.In the ubiquitination cascade,ubiquitin-conjugating enzymes(UBCs)function as ubiquitin carriers to determine linkage specificity of ubiquitin chains.In rice(Oryza sativa),39 UBC genes are identified,but only one gene OsUBC12 has been functionally studied to promote seed germination under low-temperatures in japonica rice.To elucidate the role of UBCs in seed germination,we generated CRISPR-Cas9 mutants for 23 UBC genes and overexpressed 20 members in rice.Among them,seven UBC genes(OsUBC4/6/7/12/25/27/48)were found to regulate seed germination,with OsUBC27 and OsUBC48 acting through the ABA pathway.Exogenous ABA inhibitors restored the germination rate of osubc27^(CR).RT-qPCR analysis revealed that the ABA synthesis genes OsNCED1-5 were significantly upregulated in the mutants.Further differential ubiquitination proteomics in knockout mutants and wild-type plants showed that OsUBC27 regulates ABA homeostasis by modulating ubiquitination of the ABA-degrading protein OsABA8ox1,thereby balancing seed dormancy and germination.Sequence analysis identified distinct haplotypes of the seven OsUBCs that showed differential distribution between japonica and indica subspecies.Our study provides valuable molecular targets for developing rice varieties resistant to seed vivipary.展开更多
The genetic mechanism determining amylose content(AC)and its impact on eating and cooking quality(ECQ)of rice is highly complex.To elucidate the genetic basis of AC in rice,the Ting’s core collection was used to iden...The genetic mechanism determining amylose content(AC)and its impact on eating and cooking quality(ECQ)of rice is highly complex.To elucidate the genetic basis of AC in rice,the Ting’s core collection was used to identify novel AC genes/loci through genome-wide association analysis(GWAS)using more than 5.0 million single nucleotide polymorphisms(SNPs).In this study,12 genes related to AC,including the major gene Wx and 11 minor genes,were detected using the EMMAX method.A novel gene,LR,encoding a nucleotide-binding leucine-rich-repeat(LRR)receptor(NLR)family protein,was selected for functional study.When LR was knocked out using CRISPR/Cas9,the AC decreased significantly.Furthermore,the AC in varieties was significantly higher with Haplotype A compared to Haplotypes B and C of LR.Notably,two natural variations,SNP-385(Thr-Hap.A vs Ala-Haps.B and C)and SNP-758(Ser-Hap.A vs Asn-Haps.B and C),in the coding region of LR might play critical roles in regulating AC and serve as potential targets for cultivating rice with diverse amylose contents.展开更多
Rice,a critical global staple crop,relies heavily on heading date,a key agronomic trait marking the transition from vegetative to reproductive growth.Understanding the genetic regulation of heading date is vital for e...Rice,a critical global staple crop,relies heavily on heading date,a key agronomic trait marking the transition from vegetative to reproductive growth.Understanding the genetic regulation of heading date is vital for enhancing the adaptability of high-quality rice varieties across diverse geographical regions and for bolstering local food security.In this study,we uncovered a novel role for OsCATA,a catalase gene,in the regulation of photoperiodic flowering in rice.We identified a novel allele of OsELF3.1,whose mutation resulted in delayed heading.Further analyses revealed that OsELF3.1 physically interacted with OsCATA.Notably,OsCATA exhibited rhythmic expression patterns similar to OsELF3.1 and,when mutated,also delayed flowering.Expression analyses showed that the delayed heading phenotype could be attributed to elevated Ghd7 expression under both long-day and short-day conditions,with OsCATA expression positively regulated by OsELF3.1.Double mutants of OsELF3.1 and OsCATA displayed a heading delay similar to that of oself3.1 single mutants.Additionally,OsELF3.1 could interact with Ghd7 in vivo,alleviating its suppression of Ehd1.Luciferase assays confirmed that Ghd7 repressed Ehd1 expression,while OsELF3.1 mitigated this repression.Collectively,our findings reveal that OsCATA is critical in suppressing Ghd7 expression through the OsELF3.1-OsCATA-Ghd7 transcriptional pathway,thereby regulating rice heading.展开更多
CALCINEURIN B-LIKE PROTEINS(CBLs)function in osmotic stress responses,root morphogenesis and ion uptake in various plants such as Arabidopsis.However,the roles of Os CBLs in regulating root growth in rice(Oryza sativa...CALCINEURIN B-LIKE PROTEINS(CBLs)function in osmotic stress responses,root morphogenesis and ion uptake in various plants such as Arabidopsis.However,the roles of Os CBLs in regulating root growth in rice(Oryza sativa),whose root morphology and growth environment strongly differ from those of Arabidopsis,are unknown.Here,we demonstrated that Os CBL3 functioned as a calcium sensor to regulate primary and lateral root development in rice.Os CBL3 interacted with Os CIPK31 in vivo and in vitro,and the loss of function of Os CBL3 or Os CIPK31 resulted in shorter roots and diminished lateral root growth.Overexpression of Os CIPK31 compensated for the root growth defects of Os CBL3 knockout mutants.These results demonstrated that the Os CBL3–Os CIPK31 module coordinated root development via the abscisic acid(ABA)and auxin pathways,as ABA inhibitors and low auxin concentrations partially rescued the short-root phenotype of their respective knockout lines.CYCLOPHYLIN 2(Os CYP2),a key factor in lateral root initiation and root growth maintenance,was phosphorylated by Os CIPK31,and knockout of Os CYP2 in Os CIPK31 overexpression lines resulted in a phenotype similar to that of Os CYP2 single knockout lines.Therefore,the Os CBL3–Os CIPK31 module functioned in ABA and auxin signal transduction,ensuring proper root growth.Os CIPK31,activated by Os CBL3,then phosphorylated Os CYP2,which drove primary and lateral root development.These results establish a new module regulating primary and lateral root development in rice.展开更多
Heading date is one of the most important agronomic traits that directly affect rice yield and determines the regional adaptability in specific growing environments.As a short-day plant,rice can grow under long-day(LD...Heading date is one of the most important agronomic traits that directly affect rice yield and determines the regional adaptability in specific growing environments.As a short-day plant,rice can grow under long-day(LD)conditions due to the synergistic regulation of many photosensitive genes.Using a set of chromosome segment substitution lines(CSSLs)with the indica cultivar Huanghuazhan(HHZ)as the recipient parent and Basmati Surkh 89-15(BAS)as the donor parent,we identified a QTL locus.展开更多
Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negate...Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.展开更多
Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving hig...Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving high and stable rice yields.In this study,we isolated a novel slender aerial organ mutant sao,which is characterized by a significant reduction in the width of leaves,stems,and grains.Histological analysis revealed that the slender phenotype of aerial organs in sao is caused by impaired cell proliferation and elongation.展开更多
The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrat...The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.展开更多
Rice leaf morphology is of great significance in the breeding of an ideal plant type and high photosynthetic efficiency.A forward genetics approach was employed to locate a novel gene,NRL9(NARROW AND ROLLED LEAF 9).NR...Rice leaf morphology is of great significance in the breeding of an ideal plant type and high photosynthetic efficiency.A forward genetics approach was employed to locate a novel gene,NRL9(NARROW AND ROLLED LEAF 9).NRL9 encodes a 20S proteasome β1 subunit and is involved in regulating leaf morphology and grain size.Compared with the wild type(WT),the nrl9 mutant exhibited a significant decrease in leaf width,and a significant increase in leaf rolling index(LRI),while also displaying increased grain length and decreased grain width.展开更多
The cell surface receptor chitin elicitor receptor kinase 1(CERK1)is a well-known component of plant immunity.OsCERK1 is involved in regulating copper(Cu)uptake in rice,though the underlying mechanisms remain elusive....The cell surface receptor chitin elicitor receptor kinase 1(CERK1)is a well-known component of plant immunity.OsCERK1 is involved in regulating copper(Cu)uptake in rice,though the underlying mechanisms remain elusive.In this study,we identified proteins interacting with OsCERK1 and uncovered a novel heavy metal-associated domain-containing protein,OsHPP08.Our findings demonstrate that OsCERK1 phosphorylated and stabilized OsHPP08.Through structural analysis using AlphaFold,a yeast sensitivity assay of the Cu uptake-deficient yeast mutant,and Cu level measurements in oshpp08 mutants and overexpression plants(OsHPP08OE),we revealed that OsHPP08 facilitated Cu uptake.Additionally,rice seedling infection assays demonstrated that OsHPP08 positively contributed to blast resistance,with both OsCERK1 and OsHPP08 being essential for Cu-modulated blast resistance.Further analyses suggested that OsCERK1 and OsHPP08 likely enhanced blast resistance by regulating the antioxidant system and increasing H_(2)O_(2) accumulation.In conclusion,OsCERK1 promoted Cu uptake by stabilizing OsHPP08,and together they contributed to Cu-modulated blast resistance,likely through the modulation of reactive oxygen species accumulation.These findings deepen our understanding of the intricate interplay between biotic and abiotic signals in rice.展开更多
基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202403)。
文摘Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.
基金supported by the Zhejiang Provincial Natural Science Foundation,China(Grant No.LQ24C010007)Zhejiang Science and Technology Major Program on Rice New Variety Breeding,China(Grant No.2021C02063)+4 种基金the Agricultural Sciences and Technologies Innovation Program,China(Grant No.CAAS-CSCB-202301)the Key Projects of Zhejiang Provincial Natural Science Foundation,China(Grant No.LZ23C130002)the Youth Innovation Program of Chinese Academy of Agricultural Sciences(Grant No.Y2023QC22)the Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(Grant Nos.B23YQ1514 and B23CQ15EP)the External Cooperation Projects of Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,China(Grant No.DWHZ2024-07).
文摘Rice seedling blight,caused by various fungi,including Fusarium oxysporum,poses a severe threat to rice production.As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight,there has been a shift in focus towards biological control agents.In this study,we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of F.oxysporum in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus T208.Additionally,our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual use.Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients,which were negatively correlated with the biomass of F.oxysporum-inoculated rice seedlings.Furthermore,this mixture led to higher stochastic assembly(average|βNTI|<2)and reduced selection pressures on rice rhizosphere bacteria compared with individual strain applications.The mixture of the T40 and T208 strains also significantly increased the expression of defense-related genes.In conclusion,the mixture of the T40 and T208 strains effectively modulates microbial community structures,enhances microbial network stability,and boosts the resistance against rice seedling blight.Our study supports the development and utilization of biological resources for crop protection.
基金supported by the Key Research and Development Program Project of Hunan Province, China (Grant No. 2023NK2003)the National Key Research and Development Program of China (Grant No. 2022YFD2301001-03)the National Key Research and Development Program of China (Grant No. 2022YFD2301003)
文摘The ratooning system enhances agricultural efficiency by reducing secondary sowing and resource input while maintaining rice yield parity with double cropping.However,the prolonged growth duration of the rice ratooning system extends the exposure window to Magnaporthe oryzae infection,thereby elevating the probability of disease incidence.
基金supported by the Sichuan Province International Science and Technology Innovation Cooperation(2024YFHZ0299)the Project of Science and Technology Department of Sichuan Province(2022YFH0031)Chengdu Science and Technology Bureau(2024-YF05-02168-SN).
文摘Rice sheath blight(RSB)is a major destructive disease impeding rice production.Identifying key germplasm resources with increased resistance remains a challenge.However,the mechanisms underlying disease resistance are not yet fully understood.Cytochrome P450 monooxygenases(CYP450s)serve biosynthesis and metabolic detoxification functions in plants,but there is limited information about their role in the response induced by RSB.This study demonstrated that CYT02 belongs to the CYP73A100 subfamily and is a typical member of the CYP450s.Overexpression(OE)in rice of the cytochrome P450 monooxygenase cyt02 conferred increased resistance to RSB and increased vegetative tillering.Cyt02 may increase RSB resistance by regulating plant hormone synthesis,regulate reactive oxygen species(ROS)by coordinating the activity of antioxidant enzymes,and initiate phytoalexin synthesis in response to fungal infection.These research findings have laid a foundation for a deeper understanding of the function of cyt02 and offered a potential target gene for breeding rice varieties resistant to sheath blight.
基金supported by the National Natural Science Foundation of China(Grant Nos.31972983 and 32072487)the Key Technology R&D Program of Zhejiang Province,China(Grant No.2021C02006)the Zhejiang Provincial Natural Science Foundation of China(Grant No.LY23C140001).
文摘In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial abundance and maintaining community structures.To date,only 16 rice viruses have been documented in rice-growing regions.These viruses pose serious threats to rice production and have traditionally been identified only from rice plants and insect vectors by isolation techniques.Advances in next-generation sequencing(NGS)have made it feasible to discover viruses on a global scale.Recently,numerous viruses have been identified in plants and invertebrates using NGS technologies.In this review,we discuss viral studies in rice plants,invertebrate species,and weeds in rice fields.Many novel viruses have been discovered in rice ecosystems through NGS technologies,with some also detected using metatranscriptomic and small RNA sequencing.These analyses greatly expand our understanding of viruses in rice fields and provide valuable insights for developing efficient strategies to manage insect pests and virus-mediated rice diseases.
基金supported by the National Natural Science Foundation of China(32372118 and 32188102)the Qian Qian Academician Workstation,China+3 种基金the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province,China(YSPTZX202303)the Nanfan Special Project,Chinese Academy of Agricultural Sciences(ZDXM2315)the Chinese Academy of Agricultural Sciences Talent Plan-Outstanding Young Talentthe Zhejiang Province’s High-level Talent Special Support Plan-Young Talent,China。
文摘In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tendency for high seed shattering in domesticated crops,such as rice,not only reduces paddy yield but also complicates mechanized harvesting.
基金supported by the Basic Public Welfare Research Program of Zhejiang Province,China(Grant No.LY23C130003)the Fund of the State Key Laboratory of Rice Biology and Breeding,China(Grant No.2023ZZKT20203)+5 种基金the Major Science and Technology Project of Guangxi,China(Grant No.AA23062015)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-ASTIP-2013-CNRRI)the China Rice Research System,China(Grant No.CARS-01-011)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202301)High-Quality and Resistant Hybrid Rice Germplasm Creation and New Varieties Development with International Competitiveness,China(Grant Nos.2022KJCX45 and YBXM2437)Xi’nan League Science and Technology Project,China(Grant No.2023DXZD0001).
文摘Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified the cytoplasmic ATP-citrate lyase(ACL)subunit A2 in rice(Oryza sativa L.),OsACL-A2(Os12g0566300),as a critical factor for iron uptake and transport.The osacl-a2 mutant exhibited reduced leaf iron levels,leading to iron deficiency-induced chlorosis,activated defense signaling,and eventual necrosis in mature leaves.Additionally,blast resistance was weakened in immature osacl-a2 leaves.Exogenous iron supplementation rescued these defects.The mutant displayed reduced ATP-citrate lyase activity but increased citric acid levels compared with its wild type(WT),suggesting that the osacl-a2 mutation impairs enzyme activity.Thus,OsACL-A2-mediated citrate lyase activity plays a vital role in promoting iron uptake and associated blast resistance in rice.
基金supported by the Director General,Indian Council of Agricultural Research(ICAR),New Delhithe Director,ICAR-National Rice Research Institute,Cuttack.
文摘Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,Sub1 and Dro1(Δbp:10 Mb),as well as Sub1 and TPP7(Δbp:6 Mb)were identified to exhibit long-range linkage disequilibrium(LRLD).Meta-QTL analysis further revealed that Sub1 and TPP7 co-segregated for tolerance to submergence at the germination and seedling stages.Based on this,we hypothesized that LRLD might influence plant responses to consecutive stresses.To test this hypothesis,we developed a structured recombinant inbred line population from a cross between Bhalum 2 and Nagina 22,with alleles(Sub1 and TPP7)in linkage equilibrium.Mendelian randomization analysis validated that the parental alleles,rather than the recombinant alleles of Sub1 and TPP7,significantly influenced 13 out of 41 traits under consecutive stress conditions.Additionally,16 minor additive effect QTLs were detected between the genomic regions,spanning Sub1 and TPP7 for various traits.A single allele difference between these genomic regions enhanced crown root number,root dry weight,and specific root area by 11.45%,15.69%,and 33.15%,respectively,under flooded germination conditions.Candidate gene analysis identified WAK79 and MRLK59 as regulators of stress responses during flooded germination,recovery,and subsequent water deficit conditions.These findings highlight the critical role of parental allele combinations and genomic regions between Sub1 and TPP7 in regulating the stress responses under consecutive stresses.Favourable haplotypes derived from these alleles can be utilized to improve stress resilience in direct-seeded rice.
基金supported by the National Natural Science Foundation of China(32070642 and 31371222 to Dr.Xiaoxue Wang)the National Key Research and Development Program from the Ministry of Science and Technology of China(2016YFD0100406 and 2017YFD0300107 to Dr.Xiaoxue Wang)the Science and Technology Department of Liaoning province(2022JH6/100100039 to Dr.Xiaoxue Wang)。
文摘The leaf is a major organ for photosynthesis,and its shape plays an important role in plant development and yield determination in rice(Oryza sativa L.).In this study,an adaxial curled leaf mutant,termed curly leaf 1-1(cul1-1),was obtained by chemical mutagenesis.The leaf rolling index of the cul1-1 mutant was higher than that of the wild-type,which was caused by the abnormal development of bulliform cells(BCs).We cloned the CUL1 gene by map-based cloning.A nonsense mutation was present in the cul1-1 mutant,converting a tryptophan codon into a stop codon.The CUL1 gene encodes a chromodomain,helicase/ATPase and DNA-binding domain containing protein.Genes related to leaf rolling and BC development,such as ADL1,REL1 and ROC5,were activated by the cul1-1 mutation.The trimethylation of lysine 27 in histone 3(H3K27me3),but not H3K4me3,at the ADL1,REL1 and ROC5 loci,was reduced in the cul1-1 mutant.High-throughput mRNA sequencing indicated that the cul1-1 mutation caused genome-wide differential gene expression.The differentially expressed genes were classified into a few gene ontology terms and Kyoto encyclopedia of genes and genomes pathways.In the natural population,22 missense genomic variations in the CUL1 locus were identified,which composed of 7 haplotypes.A haplotype network was also built with haplotype II as the ancestor.The findings revealed that CUL1 is essential for normal leaf development and regulates this process by inhibiting the expression of genes involved in leaf rolling and BC development.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFF1000500)the National Natural Science Foundation of China(Grant Nos.32372099,32172080,and 32188102)the Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-CSCB-202402).
文摘Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.
基金supported by the Zhejiang Provincial Natural Science Foundation,China(ZCLMS25C1302)the Central Public-interest Scientific Institution Basal Research Fund(CPSIBRF-CNRRI-202408)the Agricultural Science and Technology Innovation Program(ASTIP)
文摘Rice seed germination marks the start of cultivation and influences subsequent seedling growth,and is affected by hormones and environmental factors.Ubiquitination plays a critical role in this process by regulating hormonal homeostasis.In the ubiquitination cascade,ubiquitin-conjugating enzymes(UBCs)function as ubiquitin carriers to determine linkage specificity of ubiquitin chains.In rice(Oryza sativa),39 UBC genes are identified,but only one gene OsUBC12 has been functionally studied to promote seed germination under low-temperatures in japonica rice.To elucidate the role of UBCs in seed germination,we generated CRISPR-Cas9 mutants for 23 UBC genes and overexpressed 20 members in rice.Among them,seven UBC genes(OsUBC4/6/7/12/25/27/48)were found to regulate seed germination,with OsUBC27 and OsUBC48 acting through the ABA pathway.Exogenous ABA inhibitors restored the germination rate of osubc27^(CR).RT-qPCR analysis revealed that the ABA synthesis genes OsNCED1-5 were significantly upregulated in the mutants.Further differential ubiquitination proteomics in knockout mutants and wild-type plants showed that OsUBC27 regulates ABA homeostasis by modulating ubiquitination of the ABA-degrading protein OsABA8ox1,thereby balancing seed dormancy and germination.Sequence analysis identified distinct haplotypes of the seven OsUBCs that showed differential distribution between japonica and indica subspecies.Our study provides valuable molecular targets for developing rice varieties resistant to seed vivipary.
基金supported by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City,China(Grant No.2021JJLH0041)the Zhejiang Provincial Natural Science Foundation,China(Grant No.LY23C130006)+3 种基金the National Natural Science Foundation of China(Grant No.32472207)Nanfan Special Project,Chinese Academy of Agricultural Sciences(Grant Nos.YBXM2436 and YBXM2326)the Hainan Province Science and Technology Special Fund,China(Grant No.ZDYF2022XDNY256)the Innovational Fund for Scientific and Technological Personnel of Hainan Province,China(Grant No.KJRC2023B24).
文摘The genetic mechanism determining amylose content(AC)and its impact on eating and cooking quality(ECQ)of rice is highly complex.To elucidate the genetic basis of AC in rice,the Ting’s core collection was used to identify novel AC genes/loci through genome-wide association analysis(GWAS)using more than 5.0 million single nucleotide polymorphisms(SNPs).In this study,12 genes related to AC,including the major gene Wx and 11 minor genes,were detected using the EMMAX method.A novel gene,LR,encoding a nucleotide-binding leucine-rich-repeat(LRR)receptor(NLR)family protein,was selected for functional study.When LR was knocked out using CRISPR/Cas9,the AC decreased significantly.Furthermore,the AC in varieties was significantly higher with Haplotype A compared to Haplotypes B and C of LR.Notably,two natural variations,SNP-385(Thr-Hap.A vs Ala-Haps.B and C)and SNP-758(Ser-Hap.A vs Asn-Haps.B and C),in the coding region of LR might play critical roles in regulating AC and serve as potential targets for cultivating rice with diverse amylose contents.
基金funded by the Biological Breeding-National Science and Technology Major Projects,China(Grant No.2023ZD04066)the Zhejiang Provincial Natural Science Foundation of China(Grant Nos.LZ24C130006,LTGN24C130007)+5 种基金the Open Project Program of the State Key Laboratory of Rice Biology and Breeding,China(Grant No.20240107)the Xi’nan League Science and Technology Project,China(Grant No.2023DXZD0001)the Joint Research and Development Program on Rice Breeding in Inner Mongolia Autonomous Region,China(Grant No.YZ2023004)the China Agriculture Research System(Grant No.CARS-01)the Central Public-interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202301)the Agricultural Science and Technology Innovation Program(ASTIP).
文摘Rice,a critical global staple crop,relies heavily on heading date,a key agronomic trait marking the transition from vegetative to reproductive growth.Understanding the genetic regulation of heading date is vital for enhancing the adaptability of high-quality rice varieties across diverse geographical regions and for bolstering local food security.In this study,we uncovered a novel role for OsCATA,a catalase gene,in the regulation of photoperiodic flowering in rice.We identified a novel allele of OsELF3.1,whose mutation resulted in delayed heading.Further analyses revealed that OsELF3.1 physically interacted with OsCATA.Notably,OsCATA exhibited rhythmic expression patterns similar to OsELF3.1 and,when mutated,also delayed flowering.Expression analyses showed that the delayed heading phenotype could be attributed to elevated Ghd7 expression under both long-day and short-day conditions,with OsCATA expression positively regulated by OsELF3.1.Double mutants of OsELF3.1 and OsCATA displayed a heading delay similar to that of oself3.1 single mutants.Additionally,OsELF3.1 could interact with Ghd7 in vivo,alleviating its suppression of Ehd1.Luciferase assays confirmed that Ghd7 repressed Ehd1 expression,while OsELF3.1 mitigated this repression.Collectively,our findings reveal that OsCATA is critical in suppressing Ghd7 expression through the OsELF3.1-OsCATA-Ghd7 transcriptional pathway,thereby regulating rice heading.
基金the Sichuan Science and Technology Program(2023NSFSC1933,2022ZDZX0016,2021YFYZ0016)the Chengdu Science and Technology Bureau(2022-YF09-00036-SN)+1 种基金the free exploration project of the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKL-ZY202214)the Changde Science and Technology Bureau(changkehan 2021–59)。
文摘CALCINEURIN B-LIKE PROTEINS(CBLs)function in osmotic stress responses,root morphogenesis and ion uptake in various plants such as Arabidopsis.However,the roles of Os CBLs in regulating root growth in rice(Oryza sativa),whose root morphology and growth environment strongly differ from those of Arabidopsis,are unknown.Here,we demonstrated that Os CBL3 functioned as a calcium sensor to regulate primary and lateral root development in rice.Os CBL3 interacted with Os CIPK31 in vivo and in vitro,and the loss of function of Os CBL3 or Os CIPK31 resulted in shorter roots and diminished lateral root growth.Overexpression of Os CIPK31 compensated for the root growth defects of Os CBL3 knockout mutants.These results demonstrated that the Os CBL3–Os CIPK31 module coordinated root development via the abscisic acid(ABA)and auxin pathways,as ABA inhibitors and low auxin concentrations partially rescued the short-root phenotype of their respective knockout lines.CYCLOPHYLIN 2(Os CYP2),a key factor in lateral root initiation and root growth maintenance,was phosphorylated by Os CIPK31,and knockout of Os CYP2 in Os CIPK31 overexpression lines resulted in a phenotype similar to that of Os CYP2 single knockout lines.Therefore,the Os CBL3–Os CIPK31 module functioned in ABA and auxin signal transduction,ensuring proper root growth.Os CIPK31,activated by Os CBL3,then phosphorylated Os CYP2,which drove primary and lateral root development.These results establish a new module regulating primary and lateral root development in rice.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(Grant Nos.LZ24C130004 and LQ24C130008)。
文摘Heading date is one of the most important agronomic traits that directly affect rice yield and determines the regional adaptability in specific growing environments.As a short-day plant,rice can grow under long-day(LD)conditions due to the synergistic regulation of many photosensitive genes.Using a set of chromosome segment substitution lines(CSSLs)with the indica cultivar Huanghuazhan(HHZ)as the recipient parent and Basmati Surkh 89-15(BAS)as the donor parent,we identified a QTL locus.
基金funded by the National Natural Science Foundation of China(Grant No.32201896)the Zhejiang Province Key Research and Development Plan Project,China(Grant No.2022C02034)the National Modern Agricultural Industrial Technology System Construction Project,China(Grant No.CARS-01-21).
文摘Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.
基金supported by the Sichuan Science and Technology Program,China(Grant No.2024JDRC0064)the Chongqing Talent Program Foundation,China(Grant No.cstc2024ycjh-bgzxm0063)+1 种基金the National Natural Science Foundation of China(Grant No.32470354)the Sichuan Science and Technology Innovation and Entrepreneurship Seedling Foundation,China(Grant No.2024JDRC0064).
文摘Aerial organs in rice,including leaves,stems,and grains,are crucial for photosynthesis,lodging resistance,and yield.Therefore,an in-depth study on the development of these organs can lay a foundation for achieving high and stable rice yields.In this study,we isolated a novel slender aerial organ mutant sao,which is characterized by a significant reduction in the width of leaves,stems,and grains.Histological analysis revealed that the slender phenotype of aerial organs in sao is caused by impaired cell proliferation and elongation.
基金supported by the Major Projects in Agricultural Biological Breeding(2022ZD04002)to Xuewei ChenNational Natural Science Foundation of China(32121003 and 32425005)to Xuewei Chen+3 种基金(32172419 and 32372555)to Weitao LiCornerstone Science Foundation through the XPLORER PRIZE and New Cornerstone Investigator Program to Xuewei ChenSichuan Science and Technology Program(2023NSFSC0005)to Xuewei Chen,(2023NSFSC1996)to Weitao Li,(2023NSFSC0155)to Qingqing Hou(2024YFNH0014)to Junjie Yin.
文摘The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.
基金supported by the National Major Science and Technology Program on New Genetically Modified Organism Variety Breeding,China(Grant No.2016ZX08001-001).
文摘Rice leaf morphology is of great significance in the breeding of an ideal plant type and high photosynthetic efficiency.A forward genetics approach was employed to locate a novel gene,NRL9(NARROW AND ROLLED LEAF 9).NRL9 encodes a 20S proteasome β1 subunit and is involved in regulating leaf morphology and grain size.Compared with the wild type(WT),the nrl9 mutant exhibited a significant decrease in leaf width,and a significant increase in leaf rolling index(LRI),while also displaying increased grain length and decreased grain width.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(Grant Nos.LR24C140001 and LZ23C130002)the National Natural Science Foundation of China(Grant No.U23A20178)the Innovation Program of the Chinese Academy of Agricultural Sciences(Grant Nos.Y2023QC22 and CAAS-CSCB-202301).
文摘The cell surface receptor chitin elicitor receptor kinase 1(CERK1)is a well-known component of plant immunity.OsCERK1 is involved in regulating copper(Cu)uptake in rice,though the underlying mechanisms remain elusive.In this study,we identified proteins interacting with OsCERK1 and uncovered a novel heavy metal-associated domain-containing protein,OsHPP08.Our findings demonstrate that OsCERK1 phosphorylated and stabilized OsHPP08.Through structural analysis using AlphaFold,a yeast sensitivity assay of the Cu uptake-deficient yeast mutant,and Cu level measurements in oshpp08 mutants and overexpression plants(OsHPP08OE),we revealed that OsHPP08 facilitated Cu uptake.Additionally,rice seedling infection assays demonstrated that OsHPP08 positively contributed to blast resistance,with both OsCERK1 and OsHPP08 being essential for Cu-modulated blast resistance.Further analyses suggested that OsCERK1 and OsHPP08 likely enhanced blast resistance by regulating the antioxidant system and increasing H_(2)O_(2) accumulation.In conclusion,OsCERK1 promoted Cu uptake by stabilizing OsHPP08,and together they contributed to Cu-modulated blast resistance,likely through the modulation of reactive oxygen species accumulation.These findings deepen our understanding of the intricate interplay between biotic and abiotic signals in rice.
