BACKGROUND： Previous research has proved that nerve growth factor （NGF） participates in the onset of asthma by the induction of neurogenic inflammation. OBJECTIVE： To investigate the effect of interleukin-13 （IL...BACKGROUND： Previous research has proved that nerve growth factor （NGF） participates in the onset of asthma by the induction of neurogenic inflammation. OBJECTIVE： To investigate the effect of interleukin-13 （IL-13） and interferon- γ; （IFN- γ ） on the expression of NGF mRNA in the splenic lymphocytes of bronchial asthma rats. DESIGN, TIME AND SETTING： The experiment, a completely randomized study based on cellular immunology, was performed in the Laboratory of Neurology in Chongqing Medical University and the Department of Clinical Pharmacy in College of Clinical Medicine, Chongqing Medical University （Chongqing, China） from January 2006 to April 2007. MATERIALS： Four adult male Wistar rats were used in this study. Rat IL-13, IFN- γ probe and the total RNA extraction kit were produced by Shanghai Sangon Biological Technology ＆ Services Co., Ltd （China）. The NGF ELISA kit was a product of Wuhan Boster Bioengineering Co., Ltd （China）. A Du-70 automatic UV spectrophotometer was produced by Beckman Company （USA）. METHODS： Rats were subjected to 1-mL intraperitoneal injections each containing 100 mg of ovalbumin, and were sensitized by using antigen solution, which was sensitized with 5×10^9 Bacillus pertussis and 100 mg aluminum hydroxide powder. Four rats were challenged with 1% ovalbumin using an ultrasonic nebulizer for 60 minutes to establish an asthmatic model. After rats were anesthetized, splenic lymphocytes were isolated and cultured in medium, which was supplemented with IL-13 or IFN- γ, for 0, 12, 24 or 48 hours. A parallel study was conducted with cultured splenic lymphocytes, which were divided into a control group, an IL-13 group and an IFN- γ group. Culture medium was added with different concentrations of IL-13 （10, 50, 100 U g/L） and IFN- γ; （1, 10, 50 u g/L）; 24 hours later, all samples were harvested. MAIN OUTCOME MEASURES： The expression levels of NGF mRNA were detected by reverse transcription-polymerase chain reaction. RESULTS： In the control group, the lymphocytes of the asthmatic model expressed NGF mRNA in a time-dependent manner. Alter lymphocytes were cultured with IL-13 at a final concentration of 50 u g/L for 12, 24 or 48 hours, expression of NGF mRNA was upregulated in a time-dependent manner to higher levels than the basal values at the same time points （P 〈 0.01 ）. IL- 13 upregulated the expression of NGF mRNA in a dose-dependent manner, and the NGF mRNA expression levels at middle and high concentrations of IL-3 were significanlly higher than the values at a low concentration of IL-13 （P 〈 0.05）. With increasing IFN- γ concentration, the expression of NGF mRNA was gradually downregulated. The low concentration group showed lower levels of NGF mRNA than the blank control group, while the middle and high concentration IFN- γ, groups showed lower levels than the low concentration group （P 〈 0.05）. CONCLUSION： In asthmatic rats, IL-13, a Th2 cytokine, upregulates the expression ofNGF mRNA, while IFN- γ, a Thl cytokine, downregulates NGF mRNA expression. The effects of both cytokines were time and dose dependent. Th 1/Th2 cytokine immune imbalance may indirectly induce airway neurogenic inflammation by regulating NGF mRNA expression.展开更多
Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer(NSCLC)patients.Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC.Firstly,...Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer(NSCLC)patients.Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC.Firstly,the pemetrexed(PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines(PC-9/PEM and A549/PEM)were established.The expression of thymidylate synthase(TS)in PC-9/PEM,A549/PEM,A549,and PC-9 cells were analyzed by qRT-PCR and western blot.Then,cell viability,colony formation,migration,and invasion were performed on PEM-resistant cells transfected with TS siRNA.The role of EGFR in PEM resistance of PEM-resistant cells was investigated using EGFR siRNA.The effects of gefitinib and EGFR siRNA on EGFR/PI3K/AKT pathway and downstream signaling Cyclin D1 and E2F1 in PEM-resistant cells were analyzed.Results showed that the protein level of TS was significantly increased in A549/PEM and PC-9/PEM.TS knockdown inhibited the potency of proliferation,colony-forming potential,migration,and invasion in PEM-resistant cells.EGFR knockdown abrogated the resistance to PEM of PEM-resistant cells and suppressed the migration and invasion of PEM-resistant cells.Gefitinib treatment and EGFR knockdown respectively inhibited the EGFR/PI3K/AKT pathway and downregulated Cyclin D1 and E2F1 in PEM-resistant cells.Thus,TS might be a predictive marker for PEM resistance in NSCLC.Inhibition of the EGFR pathway abrogated the resistance to PEM and inhibited the EGFR/PI3K/AKT and downstream signaling of PEM-resistant NSCLC cell lines.展开更多
Objective To retrospectively analyze the clinical,imaging,pathological and genetic features in patients with pulmonary artery sarcoma(PAS)in a single center,and to investigate the disease origin of PAS,as well as its ...Objective To retrospectively analyze the clinical,imaging,pathological and genetic features in patients with pulmonary artery sarcoma(PAS)in a single center,and to investigate the disease origin of PAS,as well as its relationship with other pulmonary vascular diseases.Methods We retrospectively identified and analyzed clinical features of 13 cases with PAS those were admitted in the First Affiliated Hospital of Guangzhou Medical University between January 2015 and January 2021.Whole exome sequencing(WES)was performed to further analyze their genetic characteristics in8 postoperative specimens.Results The average age of PAS patients was 26-67(43.2±11.6)years,and the median time from symptom to diagnosis was 8 months(IQR:3,11.5).The most common symptom of PAS was shortness of breath(84.6%),and the most common complication was pulmonary hypertension(69.2%).A total of 5 genes with specific mutations in PAS patients were identified by genomic analysis.Compared with genetic features of pulmonary embolism(PE),pulmonary arterial hypertension(PAH)and lung cancer(LC),we found genetic similarity between PAS and LC.Using KEEG database,we identified that most of the PASmutated genes belonged to cancer-enriched signaling pathways.Conclusion PAS is a kind of malignant tumor located in the pulmonary vascular trunk,without a good prognosis and specific clinical manifstations.The occurrence of PAS may be associathed with mutations of MDM2,PIK3CA and TP53.展开更多
Objective To analyze the etiology of severe community-acquired pneumonia(SCAP)in immunocompromised patients,and to investigate the relationship between underlying diseases and infectious microorganisms.Methods A retro...Objective To analyze the etiology of severe community-acquired pneumonia(SCAP)in immunocompromised patients,and to investigate the relationship between underlying diseases and infectious microorganisms.Methods A retrospective analysis was performed on SCAP in immunocompromised patients admitted to the Fourth Department of Respiratory and Critical Medicine(MICU)of China-Japan Friendship Hospital from January 1,2017 to December 31,2019.展开更多
Objective To compare and analyze the effects of different activators on the release curve of TGF-β1 and PDGF-AB in platelet rich plasma( PRP). Methods A total of 36 ml peripheral venous blood was obtained from 10 hea...Objective To compare and analyze the effects of different activators on the release curve of TGF-β1 and PDGF-AB in platelet rich plasma( PRP). Methods A total of 36 ml peripheral venous blood was obtained from 10 healthy adult volunteers, and the PRP was made by secondary centrifugation.展开更多
基金Natural Science Foundation of Chongqing, No. [2006]24-CSTC2006EB 5030
文摘BACKGROUND： Previous research has proved that nerve growth factor （NGF） participates in the onset of asthma by the induction of neurogenic inflammation. OBJECTIVE： To investigate the effect of interleukin-13 （IL-13） and interferon- γ; （IFN- γ ） on the expression of NGF mRNA in the splenic lymphocytes of bronchial asthma rats. DESIGN, TIME AND SETTING： The experiment, a completely randomized study based on cellular immunology, was performed in the Laboratory of Neurology in Chongqing Medical University and the Department of Clinical Pharmacy in College of Clinical Medicine, Chongqing Medical University （Chongqing, China） from January 2006 to April 2007. MATERIALS： Four adult male Wistar rats were used in this study. Rat IL-13, IFN- γ probe and the total RNA extraction kit were produced by Shanghai Sangon Biological Technology ＆ Services Co., Ltd （China）. The NGF ELISA kit was a product of Wuhan Boster Bioengineering Co., Ltd （China）. A Du-70 automatic UV spectrophotometer was produced by Beckman Company （USA）. METHODS： Rats were subjected to 1-mL intraperitoneal injections each containing 100 mg of ovalbumin, and were sensitized by using antigen solution, which was sensitized with 5×10^9 Bacillus pertussis and 100 mg aluminum hydroxide powder. Four rats were challenged with 1% ovalbumin using an ultrasonic nebulizer for 60 minutes to establish an asthmatic model. After rats were anesthetized, splenic lymphocytes were isolated and cultured in medium, which was supplemented with IL-13 or IFN- γ, for 0, 12, 24 or 48 hours. A parallel study was conducted with cultured splenic lymphocytes, which were divided into a control group, an IL-13 group and an IFN- γ group. Culture medium was added with different concentrations of IL-13 （10, 50, 100 U g/L） and IFN- γ; （1, 10, 50 u g/L）; 24 hours later, all samples were harvested. MAIN OUTCOME MEASURES： The expression levels of NGF mRNA were detected by reverse transcription-polymerase chain reaction. RESULTS： In the control group, the lymphocytes of the asthmatic model expressed NGF mRNA in a time-dependent manner. Alter lymphocytes were cultured with IL-13 at a final concentration of 50 u g/L for 12, 24 or 48 hours, expression of NGF mRNA was upregulated in a time-dependent manner to higher levels than the basal values at the same time points （P 〈 0.01 ）. IL- 13 upregulated the expression of NGF mRNA in a dose-dependent manner, and the NGF mRNA expression levels at middle and high concentrations of IL-3 were significanlly higher than the values at a low concentration of IL-13 （P 〈 0.05）. With increasing IFN- γ concentration, the expression of NGF mRNA was gradually downregulated. The low concentration group showed lower levels of NGF mRNA than the blank control group, while the middle and high concentration IFN- γ, groups showed lower levels than the low concentration group （P 〈 0.05）. CONCLUSION： In asthmatic rats, IL-13, a Th2 cytokine, upregulates the expression ofNGF mRNA, while IFN- γ, a Thl cytokine, downregulates NGF mRNA expression. The effects of both cytokines were time and dose dependent. Th 1/Th2 cytokine immune imbalance may indirectly induce airway neurogenic inflammation by regulating NGF mRNA expression.
文摘Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer(NSCLC)patients.Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC.Firstly,the pemetrexed(PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines(PC-9/PEM and A549/PEM)were established.The expression of thymidylate synthase(TS)in PC-9/PEM,A549/PEM,A549,and PC-9 cells were analyzed by qRT-PCR and western blot.Then,cell viability,colony formation,migration,and invasion were performed on PEM-resistant cells transfected with TS siRNA.The role of EGFR in PEM resistance of PEM-resistant cells was investigated using EGFR siRNA.The effects of gefitinib and EGFR siRNA on EGFR/PI3K/AKT pathway and downstream signaling Cyclin D1 and E2F1 in PEM-resistant cells were analyzed.Results showed that the protein level of TS was significantly increased in A549/PEM and PC-9/PEM.TS knockdown inhibited the potency of proliferation,colony-forming potential,migration,and invasion in PEM-resistant cells.EGFR knockdown abrogated the resistance to PEM of PEM-resistant cells and suppressed the migration and invasion of PEM-resistant cells.Gefitinib treatment and EGFR knockdown respectively inhibited the EGFR/PI3K/AKT pathway and downregulated Cyclin D1 and E2F1 in PEM-resistant cells.Thus,TS might be a predictive marker for PEM resistance in NSCLC.Inhibition of the EGFR pathway abrogated the resistance to PEM and inhibited the EGFR/PI3K/AKT and downstream signaling of PEM-resistant NSCLC cell lines.
文摘Objective To retrospectively analyze the clinical,imaging,pathological and genetic features in patients with pulmonary artery sarcoma(PAS)in a single center,and to investigate the disease origin of PAS,as well as its relationship with other pulmonary vascular diseases.Methods We retrospectively identified and analyzed clinical features of 13 cases with PAS those were admitted in the First Affiliated Hospital of Guangzhou Medical University between January 2015 and January 2021.Whole exome sequencing(WES)was performed to further analyze their genetic characteristics in8 postoperative specimens.Results The average age of PAS patients was 26-67(43.2±11.6)years,and the median time from symptom to diagnosis was 8 months(IQR:3,11.5).The most common symptom of PAS was shortness of breath(84.6%),and the most common complication was pulmonary hypertension(69.2%).A total of 5 genes with specific mutations in PAS patients were identified by genomic analysis.Compared with genetic features of pulmonary embolism(PE),pulmonary arterial hypertension(PAH)and lung cancer(LC),we found genetic similarity between PAS and LC.Using KEEG database,we identified that most of the PASmutated genes belonged to cancer-enriched signaling pathways.Conclusion PAS is a kind of malignant tumor located in the pulmonary vascular trunk,without a good prognosis and specific clinical manifstations.The occurrence of PAS may be associathed with mutations of MDM2,PIK3CA and TP53.
文摘Objective To analyze the etiology of severe community-acquired pneumonia(SCAP)in immunocompromised patients,and to investigate the relationship between underlying diseases and infectious microorganisms.Methods A retrospective analysis was performed on SCAP in immunocompromised patients admitted to the Fourth Department of Respiratory and Critical Medicine(MICU)of China-Japan Friendship Hospital from January 1,2017 to December 31,2019.
文摘Objective To compare and analyze the effects of different activators on the release curve of TGF-β1 and PDGF-AB in platelet rich plasma( PRP). Methods A total of 36 ml peripheral venous blood was obtained from 10 healthy adult volunteers, and the PRP was made by secondary centrifugation.
