Prolonged exposure to teratogens is known to cause neural tube defects(NTDs),a severe malformation of the central nervous system(CNS)that significantly contributes to global infant mortality.In recent years,exposure t...Prolonged exposure to teratogens is known to cause neural tube defects(NTDs),a severe malformation of the central nervous system(CNS)that significantly contributes to global infant mortality.In recent years,exposure to nanoplastics(NPs)has been linked to faulty neural crest closure and altered neurulation by altering cellular adhesion molecules and accumulation of plastic particles in the neural tube leading to NTDs.However,research on the influence of various types of microplastics(MPs)on malformations of the CNS are still limited.In this study,we investigated whether MPs of polytetrafluoroethylene(PTFE)da type of plastic commonly used as non-stick coatings of cooking utensils can affect angiogenesis and CNS development using ducks as model organisms.PTFE MPs were administered on Day 3 of duck embryo development at varying concentrations(0.01 mg/ml,0.1 mg/ml,1 mg/ml,and 5 mg/ml),and angiogenesis was evaluated using a chorioallantoic membrane(CAM)assay.Gross morphology and histology of the spinal column and brain were analyzed on Days 8 and 18,respectively.FTIR confirmed PTFE's structure,while SEM and DLS analyses showed particle sizes between 300 nm and 5 mm,classifying them as MPs.High concentrations(5 mg/ml)of PTFE MPs treated on duck embryos resulted in a 35%mortality rate and reduced vascular density,suggesting anti-angiogenic effects.Brain and spinal abnormalities,such as encephalomalacia and spinal cord discontinuities were observed in the PTFE-treated embryos.Based on these results,PTFE is an anti-angiogenic and teratogenic agent affecting the development of duck embryos.展开更多
基金thank the Department of Research of Mindanao State University-Iligan Institute of Technology(MSU-IIT)Iligan City,Philippines(SO#00319e2024)for the financial assistancethe Philippine Council for Health Research and Development-Department of Science and Technology(PCHRD-DOST)-Phil-DIAMOND Project 2:Development of HIV Disease Models and in vitro Investigation of HIV-Related Neurocognitive and Metabolic Complications(SO#00300-2022)for the equipment utilized in this study.
文摘Prolonged exposure to teratogens is known to cause neural tube defects(NTDs),a severe malformation of the central nervous system(CNS)that significantly contributes to global infant mortality.In recent years,exposure to nanoplastics(NPs)has been linked to faulty neural crest closure and altered neurulation by altering cellular adhesion molecules and accumulation of plastic particles in the neural tube leading to NTDs.However,research on the influence of various types of microplastics(MPs)on malformations of the CNS are still limited.In this study,we investigated whether MPs of polytetrafluoroethylene(PTFE)da type of plastic commonly used as non-stick coatings of cooking utensils can affect angiogenesis and CNS development using ducks as model organisms.PTFE MPs were administered on Day 3 of duck embryo development at varying concentrations(0.01 mg/ml,0.1 mg/ml,1 mg/ml,and 5 mg/ml),and angiogenesis was evaluated using a chorioallantoic membrane(CAM)assay.Gross morphology and histology of the spinal column and brain were analyzed on Days 8 and 18,respectively.FTIR confirmed PTFE's structure,while SEM and DLS analyses showed particle sizes between 300 nm and 5 mm,classifying them as MPs.High concentrations(5 mg/ml)of PTFE MPs treated on duck embryos resulted in a 35%mortality rate and reduced vascular density,suggesting anti-angiogenic effects.Brain and spinal abnormalities,such as encephalomalacia and spinal cord discontinuities were observed in the PTFE-treated embryos.Based on these results,PTFE is an anti-angiogenic and teratogenic agent affecting the development of duck embryos.
