OBJECTIVE: To examine whether electric acupuncture can improve the daily life of patients with ischemic cerebral apoplexy at acute stage.METHODS: A stratified-block randomized controlled multicenter trial was designed...OBJECTIVE: To examine whether electric acupuncture can improve the daily life of patients with ischemic cerebral apoplexy at acute stage.METHODS: A stratified-block randomized controlled multicenter trial was designed for this study.Totally 340 patients with acute ischemic cerebral apoplexy were randomly divided into an electric acupuncture group and a control group. In the electric acupuncture group, 170 patients were treated with electric acupuncture and routine therapy, and170 patients in the control group with routine therapy alone. Major indexes for judging curative effect were Barthel index at 3- and 6- months follow-up visits and number of re-hospitalized patients. Mi-nor indexes for judging curative effect were change in the score for nervous dysfunction at 4and 12 weeks follow-up visits and number of patients persisting in rehabilitation treatment with acupuncture during follow-up visit.RESULTS: Baseline data at the time of case selection between the two groups were similar. The odds ratio(OR) was 0.92, and the 95% confidence interval(CI) was 0.49-1.73 in disabled rate and 0.73 and 0.51-1.05 in the number of re-hospitalized patients in the electric acupuncture group at 6-month follow up visit compared with the control group.There was no difference in the score for nervous dysfunction at the end of 12-week follow-up visit between the two groups. The score for nervous dysfunction at the end of 4-week treatment in the electric acupuncture group was significantly higher than that in the control group(P<0.05). The number of patients discharged from hospital who persisted in rehabilitation treatment with acupuncture in the acupuncture group was significantly higher than that in the control group.CONCLUSION: Using electric acupuncture to treat patients with acute ischemic cerebral apoplexy can effectively improve the nervous dysfunction scores after 4-week treatment and their ability to deal with daily life after 6-month follow-up visit. Systematic treatment with acupuncture may also reduce the number of patients with secondary apoplexy.展开更多
Objective:To explore the influence of scarring moxibustion on the changes of serum melatonin(MT)and neuropeptide Y(NPY)expressions in the insomnia model rats.Methods:A total of 40 SD rats were randomly divided into a ...Objective:To explore the influence of scarring moxibustion on the changes of serum melatonin(MT)and neuropeptide Y(NPY)expressions in the insomnia model rats.Methods:A total of 40 SD rats were randomly divided into a blank group,a model group,a scarring moxibustion group,an embedding needling group and a mild moxibustion group,8 rats in each group.The intraperitoneal injection of p-chlorophenylalanine(PCPA)was adopted to establish the insomnia model rats and the corresponding interventions were given,consecutively for 6 days.Separately,before modeling and the first intervention as well as before sample collection,the body weight was measured and the serum was collected in the insomnia model rats.The enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum MT and NPY.Results:Compared with the blank group,the body weight of rat in the model group was lower(P<0.01)and the levels of MT and NPY were lower in the model group(both P<0.05).Compared with the model group,in the scarring moxibustion group,the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY of the insomnia model rats were all higher(all P<0.05).Compared with the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY the insomnia model rats were all higher in the scarring moxibustion group(all P<0.05).In comparison between the embedding needling group and the mild moxibustion group,there were no statistical differences in the body weight and the levels of MT and NPY(all P>0.05).Conclusion:The regulation mechanism of sleep-wake circadian rhythm may be related to the levels of serum MT and NPY in the insomnia model rats.Scarring moxibustion increases the levels of MT and NPY more effectively and displays the effect in the intervention as compared with embedding needling and mild moxibustion in the insomnia model rats.展开更多
OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen(ST 21)and Zusanli(ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.METHODS:...OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen(ST 21)and Zusanli(ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.METHODS: Forty rats were randomly divided into four groups: negative control(NC), ulcer control(UC), acupoints of stomach meridian(ASM), and acupoints control(AC). The acute gastric ulcer model was established by bound and water immersion.Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen(ST 21) and Zusanli(ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method.The expression of tumor necrosis factor-alpha(TNF-α), apoptotic protease activating facter-1(Apaf-1), Caspase-3, p21 activated kinase 1(PAK1),extracellular regulated protein kinases 2(ERK2),phosphorylated ERK2(p ERK2), phosphoinositide3-kinase(PI3K) and RAC-alpha serine/threonine-protein kinase(Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay(ELISA).RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced(P < 0.05), while the ex-pression of PAK1, ERK2, p ERK2, PI3 K and Akt in gastric mucosa was significantly increased(P < 0.05).And ASM showed better effect than AC group(P <0.05).CONCLUSION: Moxa-burning Heat stimulating of Liangmen(ST 21) and Zusanli(ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.展开更多
Objective： To investigate the effect of direct moxibustion at Ganshu （BL18） on the serum concentrations of tumor specific growth factor （TSGF） and tumor necrosis factor a （TNF-a） in a rat model with precancerou...Objective： To investigate the effect of direct moxibustion at Ganshu （BL18） on the serum concentrations of tumor specific growth factor （TSGF） and tumor necrosis factor a （TNF-a） in a rat model with precancerous lesion of primary hepatocellular carcinoma （HCC）, so as to explore the mechanism of moxibustion underlying improvement of HCC. Methods： Sixty male Wistar rats were randomly divided into control group （n=10）, model group （n=20）, prevention group 1 （n=15） and prevention group 2 （n=15）. The normal rats were injected with physiological saline as blank control. At the same time, the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibusUon with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1 （1 treatment course, 20 days） and prevention group 2 （2 treatment courses, 40 days）, 5 doses for each acupoint, 0.5 mg/dose, once every other day. At each time point （before model establishment, the end of 1st course prevention, the end of 2rid course prevention and the end of model establishment）, serum levels of TSGF and TNF-eL were detected using enzyme-linked immunosorbent assay. Results： Compared with the control group, there was a remarkably increase of serum TSGF and TNF-eL contents in the model group at the end of the experiment （P〈0.05）. At the end of the 1st course of direct moxibustion, the contents of serum TSGF and TNF- a of rats in the prevention group 1 were significantly increased compared with that of the model group （P〈0.05）. At the end of the 2nd course of direct moxibustion, serum TSGF and TNF-a levels of rats in the model group were higher than the normal group with significantly difference （P〈0.05）, and the levels of TSGF and TNF-a in the prevention group 2 were significantly reduced in comparison with the model group （P〈0.05）. Conclusion： It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis, and the therapeutic effect of two courses were better than one course.展开更多
Objective:To observe the effect of electroacupuncture(EA)pretreatment on the protein expression of c-fos in fastigial nucleus(FN)and lateral hypothalamus area(LHA)in rats with acute myocardial ischemia-reperfusion inj...Objective:To observe the effect of electroacupuncture(EA)pretreatment on the protein expression of c-fos in fastigial nucleus(FN)and lateral hypothalamus area(LHA)in rats with acute myocardial ischemia-reperfusion injury(MIRI),and to explore the role and mechanism of FN and LHA in EA at the Heart Meridian fighting against acute MIRI reaction.Methods:Seventy Sprague-Dawley rats were randomly divided into a sham operation group,a model group,an EA-Heart Meridian group and an EA-Lung Meridian group,with 14 rats in each group;an LHA lesion plus EA-Heart Meridian group(LHA+EA-Heart Meridian group)and a FN lesion plus EA-Heart Meridian group(FN+EA-Heart Meridian group),with 7 rats in each group.Except the sham operation group,the left anterior descending branch of coronary artery was ligated to establish acute MIRI rat models in the other 5 groups.In the three groups with EA-Heart Meridian treatment,Shenmen(HT 7)and Tongli(HT 5)were selected;Taiyuan(LU 9)and Lieque(LU 7)were selected in the EA-Lung Meridian group.All the EA groups received EA stimulation prior to modeling,with 1 mA in current intensity and 2 Hz in frequency,20 min each time,once a day for a total of 7 d.The sham operation group and the model group did not receive EA stimulation.The electrocardiogram was observed in the rats to analyze the ST-segment deviation and cardiac arrhythmia score.The expression of c-fos protein in FN and LHA was detected by immunohistochemistry method.Results:Compared with the sham operation group,the ST-segment deviation,cardiac arrhythmia score and the expression of c-fos protein in the FN and LHA increased significantly in the model group(all P<0.05).Compared with the model group,the ST-segment deviation,cardiac arrhythmia score and the expression of c-fos protein in FN and LHA decreased significantly in the EA-Heart Meridian group(all P<0.05).Compared with the EA-Heart Meridian group,the ST-segment deviation and cardiac arrhythmia score increased significantly in the EA-Lung Meridian group,LHA+EA-Heart Meridian group and FN+EA-Heart Meridian group(all P<0.05);the expression of c-fos in FN increased significantly in the EA-Lung Meridian group and LHA+EA-Heart Meridian group(both P<0.05);the expression of c-fos in LHA increased significantly in the EA-Lung Meridian group and FN+EA-Heart Meridian group(both P<0.05).Conclusion:FN and LHA are involved in the mechanism of EA at Heart Meridian to improve the acute MIRI reactions,and the cerebellum may participate in the improvement of cardiac function by EA through the cerebellum-hypothalamus projection.展开更多
Objective：To observe the impacts of herb-partitioned moxibustion,warm moxibustion and electroacupuncture on the basic fibroblast growth factor（bFGF）and collagen type Ⅰ（Col Ⅰ）in colons of rats with Crohn＇ dise...Objective：To observe the impacts of herb-partitioned moxibustion,warm moxibustion and electroacupuncture on the basic fibroblast growth factor（bFGF）and collagen type Ⅰ（Col Ⅰ）in colons of rats with Crohn＇ disease（CD）,and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.Methods：The model rats were developed by TNBS as multiple proinflammatory method.The rats were randomly divided into 5 groups：a normal group,a model group,a warm moxibustion group,an electroacupuncture group and a herb-partitioned moxibustion group.The treatments were carried out at Tianshu（ST 25）（bilateral）and Qihai（CV 6）in different treatments.The immunohistochemistry was used to detect the expression position of Col Ⅰ and bFGF.Results：The expressions of Col Ⅰ and bFGF in colons of rots in the model group significantly increased（compared with the normal group,P〈0.01）.After the herb-partitioned moxibustion,warm moxibustion and electroacupuncture,the expressions of Col Ⅰ and bFGF reduced markedly in the rats with CD（P〈0.01）.The expression of bFGF and Col Ⅰ in the colons had an obvious correlation in the Spearman rank correlation analysis.Conclusion：Acupuncture treatment reduced the abnormally high levels of expressions for Col Ⅰ and bFGF in colons.Col Ⅰ and bFGF participated in the fibrosis.Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition,treating the intestinal fibrosis in CD.展开更多
Objective： The integrity of the corresponding dorsal root is important in the research of the effects of acupuncture Zusanli （ST 36） on gastric smooth muscle. Investigate the communicate channels of central control...Objective： The integrity of the corresponding dorsal root is important in the research of the effects of acupuncture Zusanli （ST 36） on gastric smooth muscle. Investigate the communicate channels of central control on the effect of acupuncture Zusanli （ST 36）. Method： Selected 30 healthy adult SD rats and using veniua-glacial acid to made gastric mucosa ulcer model. Divided them into three groups, model group, cutting L4-S1 group and transecting T10-T11 group after the models have been made successfully, each group 10 rats. Implanted diameter in 0.2 mm Platinum Circle electrode into subserous of the ulcer place and recorded the myoelectricity activity of gastric smooth muscle before and after acupuncture Zusanli （ST 36）. Acupuncture parameters, 2-100 Hz, lasting 20 min. Results： Model group before acupuncture, （5.73±0.51） cycle per minitue （cpm）, （2.28±0.57） mV; model group after acupuncture, （5.34±0.34） cpm, （1.06 ± 0.13）. Group that before cutting dorsal L4-S1, （5.65±0.26） cpm, （1.66 ±0.15） mV; group that after cutting dorsal L4-S1, （5.40 ±0.23） cpm, （1.17 ±0.23）, group that after cutting dorsal L4-S1 and acupuncture, （5.26±0.18） cpm, （2.45±0.23）. Group that before transecting dorsal T10-T11 （5.69±0.36） cpm, （1.91 ±0.25） mV, group that after transecting dorsal T10-T11, （4.98±0.53） cpm, （1.05±0.18） mV, group that after transecting dorsal T10-T11 and acupuncture, （4.60 ±0.44） cpm, （2.52 ±0.38） mV. Conclusion： Acupunture Zusanli （ST 36） can inhibit the myoelectricity activity of gastric smooth muscle, cutting L4-S1 can strengthen the myoelectricity activity, but the effect of transect T10-T11 is better. So it can be said that the integrity of Dorsal root is important to the effect of acupuncture. The two-way regulation of Acupuncture has a close relationship with the higher centre above spinal cord.展开更多
May be the important idea and practice that challenged and questioned modern science of traditional Chinese medicine (TCM) would become the resource of primary
Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AM...Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AMI）, and to understand the interaction mechanisms of cardiac sympathetic nerve in adjusting acute myocardial ischemia. Method： A total of forty healthy rabbits were selected and 8 were randomly chosen as normal control. The rest 32 rabbits were inducted acute myocardial ischemia （AMI） models by intravenous Pituitrin, then the AMI model rabbits were randomly divided into 4 groups, 8 as model group, and 8 as EA on Shenmen （HT 7） group, 8 as EA on Taixi （K/3） group, and 8 as EA on nonpoint group. BIOPAC collection system of biological signal was used to record the changes of CSNA and cardiac function, and the data was analyzed using a SKY-A8 disposal system. Result： The lefl ventricular systolic peak pressure （LVSP）, maximum rate of rise of LVSP （＋dp/dt max） descended obviously, and maximum rate of descent of LVSP （-dp/dt max） ascended after induction of AMI. The ＋dp/dt max, -dp/dt max and LVSP of EA on Shenmen （HT 7） group are significantly higher than the untreated AMI group, EA on Taixi （KI 3） group and EA on nonpoint group after EA. The frequency of cardiac sympathetic nerve activity increased significantly after induction of AMI, and then decreased sharply （compared with that of normal group, P〈0.05）. Following EA, the cardiac sympathetic nerve activity of EA on Shenmen （HT 7） group are significantly higher than the model group, EA on Taixi （KI 3） group and EA on nonpoint group. And they have no significant difference with normal group. Conclusion： EA on Shenmen （HT 7） could improve the cardiac function and cardiac sympathetic nerve activity of rabbits with AMI, and promote the restoration of cardiac function. There is specificity in the effects of EA on different Yuan-Primary acupoints with the best effect on Shenmen （HT 7）, and no effect on Taixi （KI 3） and nonpoint. EA on related acupoint can adjust the cardiac sympathetic nerve activity, and may moderate the effects of myocardial ischemia. The sympathetic nerve is one of the key periphery ways in using acupuncture against myocardial ischemia.展开更多
Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmeno...Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmenorrhea were randomly allocated into a group of single point. In each treatment, the needles retained for 30 min and 15 person times were observed in each group. The VAS （Visual Analogue Scale） values were recorded immediately before needling, 5 min, 20 min and 30 min upon insertion, and 30 min, 60 min and 120 min upon withdrawal for statistical analysis. Results： Immediate pain relief occurred following acupuncture and the pain-alleviating effect was incremented within the 30 min of needle retaining. At 20 min upon needle insertion, the pain relief from needling multiple points appeared substantially stronger than needling a single point （P〈0.05） and this effect remained until 2 h after withdraw of the needles. Conclusion： Needling a single point or multiple points can both produce remarkably immediate pain relief for patients with severe primary dysmenorrhea and share similar time-effect relationship. However, needling multiple points showed substantially better effects than needling a single point in a number of time points.展开更多
Objective： To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern, the authors applied gene chip tech to filter variably expressed genes in hypoth...Objective： To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern, the authors applied gene chip tech to filter variably expressed genes in hypothalamus. Methods： Rats were seperated into normal, model, acupuncture Heart Meridian group and acup Lung Meridian group randomly. Acute myocardial ischemia rat model was made with ligation left anterior descending branch of the coronary artery. After model succeed, select hypothalamus seperately and mixed the same group together of 3 rats. Then applied Rats U230A genechip refered by Affymetrix Co. to compare the variations between these groups. Results： To compare with normal group, differential expression genes and expression sequence tags （ESTs） in model group were 73 with signal log ratio ≥ 1 and 92 with signal log ratio ≤-1 , mainly included ion channel, calcium/ calmodulin-dependent protein kinase Ⅱ inhibitor, antigen and so on. Similarly, compared with model group, differential expression genes and expression sequence tags （ESTs） were 190 with signal log ratio ≥ 1 and 34 with signal log ratio ≤-1 in acupuncture Heart Meridian group, mainly included 5-hydroxytryptamine receptor, cellular metabolism, fatty, immuno reaction, G-protein coupled receptors, ion transport, signal transductions and so on, while in acupucnturc Lung Meridian, the number is 57 and 26 correspondly. Conclusion： There were exactly variations in hypothalamus mechanism that relate to acupuncture Heart and Lung Meridians.展开更多
Objective:There has been little research on effects of electroacupuncture (EA) of different acupoints on cardiac sympathetic nerve activity (CSNA).This study investigates the different effects of
Objective： To study the influence of different methods on serum soluble intercellular adhesion molecule1 （sICAM1） in patients with acute cerebral infarction. Methods： Double antibody enzyme-linked immunoadsorbent ...Objective： To study the influence of different methods on serum soluble intercellular adhesion molecule1 （sICAM1） in patients with acute cerebral infarction. Methods： Double antibody enzyme-linked immunoadsorbent assay was used. Results and Conclusion： Scalp point-to-point acupuncture has a significant reducing effect on abnormally elevated serum sICAM1 in patients with acute cerebral infarction. Its curative effect is superior to that of simple Western medicine. There is a significant difference between them （P〈 0,05）.展开更多
Objective： The present study is to investigate the mechanism of dectrtyacupuncture in preventing and treating lumbar intervertebral disk displacement. Methods： Seventy-five Sprauge-Dawley rats were used to establish...Objective： The present study is to investigate the mechanism of dectrtyacupuncture in preventing and treating lumbar intervertebral disk displacement. Methods： Seventy-five Sprauge-Dawley rats were used to establish the models of lumbar intervertebral disk displacement by operation, and divided into five groups including electro-acupuncture group, western medicine group, acupuncture plus medicine group, model group and normal control group, then they were treated with corresponding therapeutic method. The expression of inducible nitric oxide synthase （iNOS） mRNA in the lumbar intervertebral disk tissues of rats was detected by RTPCR. Results： The expression of iNOS mRNA in the lumbar intervertebral disk tissues of rats in model group was compared with that in normal control group, the significant difference was found （P〈 0.01）; While the expressions of iNOS mRNA in the lumbar intervertebral disk tissues of rats in electroacupuncture group, western drug group and acupuncture plus medicine group were all inhibited, and compared with the model group, the significant differences were also found （P 〈 0.01）. Conclusion： Electro-acupuncture could inhibit the expression of iNOS mRNA in the lumbar intervertebral disk tissue.展开更多
Objective： To investigate the clinical efficacy of needling Danzhong（CV 17） in the treatment of postpartum hypogalactia and provide clinical evidence for indications of the point. Methods： A multi-centre single-bl...Objective： To investigate the clinical efficacy of needling Danzhong（CV 17） in the treatment of postpartum hypogalactia and provide clinical evidence for indications of the point. Methods： A multi-centre single-blind randomized controlled trial was carried out. Two hundred and seventy-six puerperal women with postpartum hypogalactia were randomly allocated into acupuncture group and herb group, and respectively treated for three consecutive days. The degree of mammary fullness, the amount of milk secreted, prolacfin, baby weight, the frequency and volume of artificial feeding, the number of infant urination events, and the duration of baby crying were observed. The clinical curative effects on postpartum hypogalactia were compared. Results： Hypogalactia was effectively treated in both acupuncture and herb groups. There were statistically significant differences in degree of mammary fullness, amount of milk secreted, baby weight, the frequency and amount of artificial feeding, and the number of infant urination events between pretreatment and post-treatment, but no difference between the two groups. There was no significant difference in prolactin in the acupuncture group and there was a difference in prolactin in the herb group between pretreatment and posttreatment. Conclusion： Needling Danzhong（CV 17） can effectively promote lactation.展开更多
基金Chongqing Municipal Planned the Sci-tech Project(#CSTC,No.2011AC5187)
文摘OBJECTIVE: To examine whether electric acupuncture can improve the daily life of patients with ischemic cerebral apoplexy at acute stage.METHODS: A stratified-block randomized controlled multicenter trial was designed for this study.Totally 340 patients with acute ischemic cerebral apoplexy were randomly divided into an electric acupuncture group and a control group. In the electric acupuncture group, 170 patients were treated with electric acupuncture and routine therapy, and170 patients in the control group with routine therapy alone. Major indexes for judging curative effect were Barthel index at 3- and 6- months follow-up visits and number of re-hospitalized patients. Mi-nor indexes for judging curative effect were change in the score for nervous dysfunction at 4and 12 weeks follow-up visits and number of patients persisting in rehabilitation treatment with acupuncture during follow-up visit.RESULTS: Baseline data at the time of case selection between the two groups were similar. The odds ratio(OR) was 0.92, and the 95% confidence interval(CI) was 0.49-1.73 in disabled rate and 0.73 and 0.51-1.05 in the number of re-hospitalized patients in the electric acupuncture group at 6-month follow up visit compared with the control group.There was no difference in the score for nervous dysfunction at the end of 12-week follow-up visit between the two groups. The score for nervous dysfunction at the end of 4-week treatment in the electric acupuncture group was significantly higher than that in the control group(P<0.05). The number of patients discharged from hospital who persisted in rehabilitation treatment with acupuncture in the acupuncture group was significantly higher than that in the control group.CONCLUSION: Using electric acupuncture to treat patients with acute ischemic cerebral apoplexy can effectively improve the nervous dysfunction scores after 4-week treatment and their ability to deal with daily life after 6-month follow-up visit. Systematic treatment with acupuncture may also reduce the number of patients with secondary apoplexy.
基金Supported by Youth Fund of National Natural Science Foundation of China:No.81704183Henan Science and Technology Public relations program project:No.172102310014+1 种基金Special project of Henan province for Scientific Research of Traditional Chinese Medicine:No.2017ZY1021Special project of Henan Province for Scientific Research of Traditional Chinese Medicine:No.2018ZY1015.
文摘Objective:To explore the influence of scarring moxibustion on the changes of serum melatonin(MT)and neuropeptide Y(NPY)expressions in the insomnia model rats.Methods:A total of 40 SD rats were randomly divided into a blank group,a model group,a scarring moxibustion group,an embedding needling group and a mild moxibustion group,8 rats in each group.The intraperitoneal injection of p-chlorophenylalanine(PCPA)was adopted to establish the insomnia model rats and the corresponding interventions were given,consecutively for 6 days.Separately,before modeling and the first intervention as well as before sample collection,the body weight was measured and the serum was collected in the insomnia model rats.The enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum MT and NPY.Results:Compared with the blank group,the body weight of rat in the model group was lower(P<0.01)and the levels of MT and NPY were lower in the model group(both P<0.05).Compared with the model group,in the scarring moxibustion group,the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY of the insomnia model rats were all higher(all P<0.05).Compared with the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY the insomnia model rats were all higher in the scarring moxibustion group(all P<0.05).In comparison between the embedding needling group and the mild moxibustion group,there were no statistical differences in the body weight and the levels of MT and NPY(all P>0.05).Conclusion:The regulation mechanism of sleep-wake circadian rhythm may be related to the levels of serum MT and NPY in the insomnia model rats.Scarring moxibustion increases the levels of MT and NPY more effectively and displays the effect in the intervention as compared with embedding needling and mild moxibustion in the insomnia model rats.
基金National Natural Science Foundation of China:To Study the Pre-protection Mechanism of Moxibustion Treatment of Acupoints of Stomach Meridian Regulating Egfr Signaling Pathway in Chronic Atrophic Gastritis Precancerous Lesions(No.81260556)National Natural Science Foundation of China:to Study Brain-stomach Response Mechanism of Electro-acupuncture Treatment of Acupoints of Stomach Meridian Repairing Gastric Mucosa Damage Based on Nuclear Magnetic Resonance Metabonomics Technology(No.81473751)+1 种基金the National Key Basic Research Development Plan(973 Plan):the Basic and Clinical Research of Moxibustion and Acupuncture Effect Characteristics and the Similarities and Differences of Their Effect Response(2015cb554502)Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China:to Study the Mechanism of Compatibility of Yiqi And Huoxue Traditional Chinese Herbs Selective Amplifying Regulatory T Cells to Inhibit Atherosclerosis(No.2015-311)
文摘OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen(ST 21)and Zusanli(ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer.METHODS: Forty rats were randomly divided into four groups: negative control(NC), ulcer control(UC), acupoints of stomach meridian(ASM), and acupoints control(AC). The acute gastric ulcer model was established by bound and water immersion.Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen(ST 21) and Zusanli(ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method.The expression of tumor necrosis factor-alpha(TNF-α), apoptotic protease activating facter-1(Apaf-1), Caspase-3, p21 activated kinase 1(PAK1),extracellular regulated protein kinases 2(ERK2),phosphorylated ERK2(p ERK2), phosphoinositide3-kinase(PI3K) and RAC-alpha serine/threonine-protein kinase(Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay(ELISA).RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced(P < 0.05), while the ex-pression of PAK1, ERK2, p ERK2, PI3 K and Akt in gastric mucosa was significantly increased(P < 0.05).And ASM showed better effect than AC group(P <0.05).CONCLUSION: Moxa-burning Heat stimulating of Liangmen(ST 21) and Zusanli(ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.
基金Supported by the National Natural Science Foundation of China(No.81202760)the Doctoral Program of Higher Educition of Ministry Education of China(No.20100013120014)
文摘Objective： To investigate the effect of direct moxibustion at Ganshu （BL18） on the serum concentrations of tumor specific growth factor （TSGF） and tumor necrosis factor a （TNF-a） in a rat model with precancerous lesion of primary hepatocellular carcinoma （HCC）, so as to explore the mechanism of moxibustion underlying improvement of HCC. Methods： Sixty male Wistar rats were randomly divided into control group （n=10）, model group （n=20）, prevention group 1 （n=15） and prevention group 2 （n=15）. The normal rats were injected with physiological saline as blank control. At the same time, the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibusUon with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1 （1 treatment course, 20 days） and prevention group 2 （2 treatment courses, 40 days）, 5 doses for each acupoint, 0.5 mg/dose, once every other day. At each time point （before model establishment, the end of 1st course prevention, the end of 2rid course prevention and the end of model establishment）, serum levels of TSGF and TNF-eL were detected using enzyme-linked immunosorbent assay. Results： Compared with the control group, there was a remarkably increase of serum TSGF and TNF-eL contents in the model group at the end of the experiment （P〈0.05）. At the end of the 1st course of direct moxibustion, the contents of serum TSGF and TNF- a of rats in the prevention group 1 were significantly increased compared with that of the model group （P〈0.05）. At the end of the 2nd course of direct moxibustion, serum TSGF and TNF-a levels of rats in the model group were higher than the normal group with significantly difference （P〈0.05）, and the levels of TSGF and TNF-a in the prevention group 2 were significantly reduced in comparison with the model group （P〈0.05）. Conclusion： It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis, and the therapeutic effect of two courses were better than one course.
文摘Objective:To observe the effect of electroacupuncture(EA)pretreatment on the protein expression of c-fos in fastigial nucleus(FN)and lateral hypothalamus area(LHA)in rats with acute myocardial ischemia-reperfusion injury(MIRI),and to explore the role and mechanism of FN and LHA in EA at the Heart Meridian fighting against acute MIRI reaction.Methods:Seventy Sprague-Dawley rats were randomly divided into a sham operation group,a model group,an EA-Heart Meridian group and an EA-Lung Meridian group,with 14 rats in each group;an LHA lesion plus EA-Heart Meridian group(LHA+EA-Heart Meridian group)and a FN lesion plus EA-Heart Meridian group(FN+EA-Heart Meridian group),with 7 rats in each group.Except the sham operation group,the left anterior descending branch of coronary artery was ligated to establish acute MIRI rat models in the other 5 groups.In the three groups with EA-Heart Meridian treatment,Shenmen(HT 7)and Tongli(HT 5)were selected;Taiyuan(LU 9)and Lieque(LU 7)were selected in the EA-Lung Meridian group.All the EA groups received EA stimulation prior to modeling,with 1 mA in current intensity and 2 Hz in frequency,20 min each time,once a day for a total of 7 d.The sham operation group and the model group did not receive EA stimulation.The electrocardiogram was observed in the rats to analyze the ST-segment deviation and cardiac arrhythmia score.The expression of c-fos protein in FN and LHA was detected by immunohistochemistry method.Results:Compared with the sham operation group,the ST-segment deviation,cardiac arrhythmia score and the expression of c-fos protein in the FN and LHA increased significantly in the model group(all P<0.05).Compared with the model group,the ST-segment deviation,cardiac arrhythmia score and the expression of c-fos protein in FN and LHA decreased significantly in the EA-Heart Meridian group(all P<0.05).Compared with the EA-Heart Meridian group,the ST-segment deviation and cardiac arrhythmia score increased significantly in the EA-Lung Meridian group,LHA+EA-Heart Meridian group and FN+EA-Heart Meridian group(all P<0.05);the expression of c-fos in FN increased significantly in the EA-Lung Meridian group and LHA+EA-Heart Meridian group(both P<0.05);the expression of c-fos in LHA increased significantly in the EA-Lung Meridian group and FN+EA-Heart Meridian group(both P<0.05).Conclusion:FN and LHA are involved in the mechanism of EA at Heart Meridian to improve the acute MIRI reactions,and the cerebellum may participate in the improvement of cardiac function by EA through the cerebellum-hypothalamus projection.
基金National Basic Research Program of China(973Program,2009CB522900)National Natural Science Foundation of China(30973785)The Key Laboratory of Acupuncture-immune Effects of State Administration of Traditional Chinese Medicine
文摘Objective：To observe the impacts of herb-partitioned moxibustion,warm moxibustion and electroacupuncture on the basic fibroblast growth factor（bFGF）and collagen type Ⅰ（Col Ⅰ）in colons of rats with Crohn＇ disease（CD）,and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.Methods：The model rats were developed by TNBS as multiple proinflammatory method.The rats were randomly divided into 5 groups：a normal group,a model group,a warm moxibustion group,an electroacupuncture group and a herb-partitioned moxibustion group.The treatments were carried out at Tianshu（ST 25）（bilateral）and Qihai（CV 6）in different treatments.The immunohistochemistry was used to detect the expression position of Col Ⅰ and bFGF.Results：The expressions of Col Ⅰ and bFGF in colons of rots in the model group significantly increased（compared with the normal group,P〈0.01）.After the herb-partitioned moxibustion,warm moxibustion and electroacupuncture,the expressions of Col Ⅰ and bFGF reduced markedly in the rats with CD（P〈0.01）.The expression of bFGF and Col Ⅰ in the colons had an obvious correlation in the Spearman rank correlation analysis.Conclusion：Acupuncture treatment reduced the abnormally high levels of expressions for Col Ⅰ and bFGF in colons.Col Ⅰ and bFGF participated in the fibrosis.Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition,treating the intestinal fibrosis in CD.
文摘Objective： The integrity of the corresponding dorsal root is important in the research of the effects of acupuncture Zusanli （ST 36） on gastric smooth muscle. Investigate the communicate channels of central control on the effect of acupuncture Zusanli （ST 36）. Method： Selected 30 healthy adult SD rats and using veniua-glacial acid to made gastric mucosa ulcer model. Divided them into three groups, model group, cutting L4-S1 group and transecting T10-T11 group after the models have been made successfully, each group 10 rats. Implanted diameter in 0.2 mm Platinum Circle electrode into subserous of the ulcer place and recorded the myoelectricity activity of gastric smooth muscle before and after acupuncture Zusanli （ST 36）. Acupuncture parameters, 2-100 Hz, lasting 20 min. Results： Model group before acupuncture, （5.73±0.51） cycle per minitue （cpm）, （2.28±0.57） mV; model group after acupuncture, （5.34±0.34） cpm, （1.06 ± 0.13）. Group that before cutting dorsal L4-S1, （5.65±0.26） cpm, （1.66 ±0.15） mV; group that after cutting dorsal L4-S1, （5.40 ±0.23） cpm, （1.17 ±0.23）, group that after cutting dorsal L4-S1 and acupuncture, （5.26±0.18） cpm, （2.45±0.23）. Group that before transecting dorsal T10-T11 （5.69±0.36） cpm, （1.91 ±0.25） mV, group that after transecting dorsal T10-T11, （4.98±0.53） cpm, （1.05±0.18） mV, group that after transecting dorsal T10-T11 and acupuncture, （4.60 ±0.44） cpm, （2.52 ±0.38） mV. Conclusion： Acupunture Zusanli （ST 36） can inhibit the myoelectricity activity of gastric smooth muscle, cutting L4-S1 can strengthen the myoelectricity activity, but the effect of transect T10-T11 is better. So it can be said that the integrity of Dorsal root is important to the effect of acupuncture. The two-way regulation of Acupuncture has a close relationship with the higher centre above spinal cord.
文摘May be the important idea and practice that challenged and questioned modern science of traditional Chinese medicine (TCM) would become the resource of primary
基金National Basic Research Program of China (973 Program, 2005CB523308)University Science Research Project of Anhui Province (KJ2009B200Z)
文摘Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AMI）, and to understand the interaction mechanisms of cardiac sympathetic nerve in adjusting acute myocardial ischemia. Method： A total of forty healthy rabbits were selected and 8 were randomly chosen as normal control. The rest 32 rabbits were inducted acute myocardial ischemia （AMI） models by intravenous Pituitrin, then the AMI model rabbits were randomly divided into 4 groups, 8 as model group, and 8 as EA on Shenmen （HT 7） group, 8 as EA on Taixi （K/3） group, and 8 as EA on nonpoint group. BIOPAC collection system of biological signal was used to record the changes of CSNA and cardiac function, and the data was analyzed using a SKY-A8 disposal system. Result： The lefl ventricular systolic peak pressure （LVSP）, maximum rate of rise of LVSP （＋dp/dt max） descended obviously, and maximum rate of descent of LVSP （-dp/dt max） ascended after induction of AMI. The ＋dp/dt max, -dp/dt max and LVSP of EA on Shenmen （HT 7） group are significantly higher than the untreated AMI group, EA on Taixi （KI 3） group and EA on nonpoint group after EA. The frequency of cardiac sympathetic nerve activity increased significantly after induction of AMI, and then decreased sharply （compared with that of normal group, P〈0.05）. Following EA, the cardiac sympathetic nerve activity of EA on Shenmen （HT 7） group are significantly higher than the model group, EA on Taixi （KI 3） group and EA on nonpoint group. And they have no significant difference with normal group. Conclusion： EA on Shenmen （HT 7） could improve the cardiac function and cardiac sympathetic nerve activity of rabbits with AMI, and promote the restoration of cardiac function. There is specificity in the effects of EA on different Yuan-Primary acupoints with the best effect on Shenmen （HT 7）, and no effect on Taixi （KI 3） and nonpoint. EA on related acupoint can adjust the cardiac sympathetic nerve activity, and may moderate the effects of myocardial ischemia. The sympathetic nerve is one of the key periphery ways in using acupuncture against myocardial ischemia.
基金Key Project of the National Eleventh-Five Year Research Program of China (2006BAI12B06)Shandong Science & Technology Development Program of Traditional Chinese Medicine (2009-135)
文摘Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmenorrhea were randomly allocated into a group of single point. In each treatment, the needles retained for 30 min and 15 person times were observed in each group. The VAS （Visual Analogue Scale） values were recorded immediately before needling, 5 min, 20 min and 30 min upon insertion, and 30 min, 60 min and 120 min upon withdrawal for statistical analysis. Results： Immediate pain relief occurred following acupuncture and the pain-alleviating effect was incremented within the 30 min of needle retaining. At 20 min upon needle insertion, the pain relief from needling multiple points appeared substantially stronger than needling a single point （P〈0.05） and this effect remained until 2 h after withdraw of the needles. Conclusion： Needling a single point or multiple points can both produce remarkably immediate pain relief for patients with severe primary dysmenorrhea and share similar time-effect relationship. However, needling multiple points showed substantially better effects than needling a single point in a number of time points.
文摘Objective： To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern, the authors applied gene chip tech to filter variably expressed genes in hypothalamus. Methods： Rats were seperated into normal, model, acupuncture Heart Meridian group and acup Lung Meridian group randomly. Acute myocardial ischemia rat model was made with ligation left anterior descending branch of the coronary artery. After model succeed, select hypothalamus seperately and mixed the same group together of 3 rats. Then applied Rats U230A genechip refered by Affymetrix Co. to compare the variations between these groups. Results： To compare with normal group, differential expression genes and expression sequence tags （ESTs） in model group were 73 with signal log ratio ≥ 1 and 92 with signal log ratio ≤-1 , mainly included ion channel, calcium/ calmodulin-dependent protein kinase Ⅱ inhibitor, antigen and so on. Similarly, compared with model group, differential expression genes and expression sequence tags （ESTs） were 190 with signal log ratio ≥ 1 and 34 with signal log ratio ≤-1 in acupuncture Heart Meridian group, mainly included 5-hydroxytryptamine receptor, cellular metabolism, fatty, immuno reaction, G-protein coupled receptors, ion transport, signal transductions and so on, while in acupucnturc Lung Meridian, the number is 57 and 26 correspondly. Conclusion： There were exactly variations in hypothalamus mechanism that relate to acupuncture Heart and Lung Meridians.
文摘Objective:There has been little research on effects of electroacupuncture (EA) of different acupoints on cardiac sympathetic nerve activity (CSNA).This study investigates the different effects of
文摘Objective： To study the influence of different methods on serum soluble intercellular adhesion molecule1 （sICAM1） in patients with acute cerebral infarction. Methods： Double antibody enzyme-linked immunoadsorbent assay was used. Results and Conclusion： Scalp point-to-point acupuncture has a significant reducing effect on abnormally elevated serum sICAM1 in patients with acute cerebral infarction. Its curative effect is superior to that of simple Western medicine. There is a significant difference between them （P〈 0,05）.
文摘Objective： The present study is to investigate the mechanism of dectrtyacupuncture in preventing and treating lumbar intervertebral disk displacement. Methods： Seventy-five Sprauge-Dawley rats were used to establish the models of lumbar intervertebral disk displacement by operation, and divided into five groups including electro-acupuncture group, western medicine group, acupuncture plus medicine group, model group and normal control group, then they were treated with corresponding therapeutic method. The expression of inducible nitric oxide synthase （iNOS） mRNA in the lumbar intervertebral disk tissues of rats was detected by RTPCR. Results： The expression of iNOS mRNA in the lumbar intervertebral disk tissues of rats in model group was compared with that in normal control group, the significant difference was found （P〈 0.01）; While the expressions of iNOS mRNA in the lumbar intervertebral disk tissues of rats in electroacupuncture group, western drug group and acupuncture plus medicine group were all inhibited, and compared with the model group, the significant differences were also found （P 〈 0.01）. Conclusion： Electro-acupuncture could inhibit the expression of iNOS mRNA in the lumbar intervertebral disk tissue.
基金Chinese Medicine Administration Bureau Special Clinical Study Fund for Acupoint Dictionary of People's Republic of China National Traditional(03XDLZ09)
文摘Objective： To investigate the clinical efficacy of needling Danzhong（CV 17） in the treatment of postpartum hypogalactia and provide clinical evidence for indications of the point. Methods： A multi-centre single-blind randomized controlled trial was carried out. Two hundred and seventy-six puerperal women with postpartum hypogalactia were randomly allocated into acupuncture group and herb group, and respectively treated for three consecutive days. The degree of mammary fullness, the amount of milk secreted, prolacfin, baby weight, the frequency and volume of artificial feeding, the number of infant urination events, and the duration of baby crying were observed. The clinical curative effects on postpartum hypogalactia were compared. Results： Hypogalactia was effectively treated in both acupuncture and herb groups. There were statistically significant differences in degree of mammary fullness, amount of milk secreted, baby weight, the frequency and amount of artificial feeding, and the number of infant urination events between pretreatment and post-treatment, but no difference between the two groups. There was no significant difference in prolactin in the acupuncture group and there was a difference in prolactin in the herb group between pretreatment and posttreatment. Conclusion： Needling Danzhong（CV 17） can effectively promote lactation.
