Objective:To characterize placental morphologic features in Moroccan women with adverse outcomes,across different clinical contexts,based on the Amsterdam consensus classification.Methods:A prospective analysis was co...Objective:To characterize placental morphologic features in Moroccan women with adverse outcomes,across different clinical contexts,based on the Amsterdam consensus classification.Methods:A prospective analysis was conducted on placentas with umbilical cords collected fresh between March 1,2024 and July 15,2024 from women with adverse pregnancy outcomes.Clinical data(age,parity,gravidity,complications)were retrieved.Macroscopic parameters(weight,dimensions,cord insertion,membranes,lesions)were assessed,followed by systematic sampling.Tissue was processed by standard histology(formalin fixation,paraffin embedding,hematoxylin and eosin staining),and lesions were classified per Amsterdam criteria.Results:16 placentas from patients with adverse pregnancy outcomes were included.The median maternal age was 30 years.Adverse conditions included placental abruption(50%),intrauterine growth restriction(IUGR,38%),intrauterine fetal death(IUFD,31%),pre-eclampsia/eclampsia(19%),premature rupture of membranes(13%),and oligohydramnios(13%).Several placentas were associated with more than one adverse condition.Histopathology revealed maternal vascular malperfusion lesions in 94%,particularly in pre-eclampsia,IUGR,and IUFD.Fetal vascular malperfusion was found in 88%,mainly in IUGR and IUFD.Inflammatory lesions,dominated by acute maternal and fetal responses stage 3(necrotizing chorioamnionitis and funisitis),were primarily linked to IUFD.Conclusions:Placental examination enhances understanding of the pathophysiology underlying adverse pregnancy outcomes,supports diagnostic confirmation,and guides preventive strategies for recurrence.This study highlights the prevalence of maternal vascular malperfusion in Moroccan women and emphasizes the importance of systematic placental histopathology in obstetric care.展开更多
Objectives:Cancer treatment relies heavily on accurate diagnosis and effective monitoring of the disease.These processes often involve invasive procedures,such as colonoscopy,to detect malignant tissues,followed by mo...Objectives:Cancer treatment relies heavily on accurate diagnosis and effective monitoring of the disease.These processes often involve invasive procedures,such as colonoscopy,to detect malignant tissues,followed by molecular analyses to determine relevant biomarkers.This study aimed to evaluate the clinical performance of droplet digital PCR(ddPCR)for detecting Kirsten Rat Sarcoma Viral Proto-Oncogene(KRAS),Neuroblastoma RAS Viral Oncogene Homolog(NRAS),and B-Raf Murine Sarcoma Viral Oncogene Homolog B(BRAF)mutations in circulating tumor DNA(ctDNA)from colorectal cancer patients using liquid biopsy.Methods:ctDNA was isolated from colorectal cancer(CRC)patients(n=110)and analyzed for KRAS,BRAF,and NRAS mutations.The ctDNA obtained through liquid biopsy was analyzed using ddPCR,and the findings were compared with sequencing data from tumor DNA archived in formalin-fixed paraffin-embedded(FFPE)blocks.Results:For KRAS mutations,ddPCR achieved a sensitivity of 72.0%and a specificity of 71.4%.However,when pooling all target mutations(KRAS,NRAS and BRAF),the overall sensitivity and specificity were lower,at 48.3%and 51.1%,respectively.Conclusion:The results of this study indicate that the ddPCR analysis of ctDNA may provide complementary information for the molecular diagnosis of CRC patients.展开更多
The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside thei...The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments.One such species,B.calcarea,has been described from the Chandalaz Mountains in northeastern Asia(Russia).This study aimed to investigate the genome structure and evolutionary history of B.calcarea.However,our analyses reveal that the species does not belong to Boechera.Instead,an integrative approach combining cytogenetic,phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae.The diploid Parryodes calcarea(2n=16)exhibits Arabideae-specific chromosomal signatures,including multiple centromere repositionings.These findings clarify the misclassification of P.calcarea as Boechera,leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World.This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe,the Arabideae.展开更多
Like-charge pairing is a physical manifestation of the unique solvation properties of certain ion pairs in water.Water's high dielectric constant and related charge screening capability significantly influence the...Like-charge pairing is a physical manifestation of the unique solvation properties of certain ion pairs in water.Water's high dielectric constant and related charge screening capability significantly influence the interaction between like-charged ions,with the possibility to transform it-in exceptional cases when noncovalent interactions are involved-from repulsion to attraction.Guanidinium cations(Gdm^(+))represent a quintessential example of such like-charge pairing due to their specific geometry and electronic structure.In this work,we present experimental validation and quantification of Gdm^(+)-Gdm contact ion pairing in water utilizing nuclear magnetic resonance(NMR)spectroscopy complemented by molecular dynamics(MD)simulations and density functional theory(DFT)calculations.The observed Gdm^(+)-Gdm^(+)interaction is attractive albeit weak-about 0.5 kJ·mol^(-1)-which aligns with theoretical estimation from MD simulations.We contrast the behavior of Gdm^(+) with that of NH_(4)^(+) cations,which exhibit no contact ion pairing in water.DFT calculations predict that the NMR chemical shift of Gdm^(+) dimers is different than that of monomers,in agreement with NMR titration curves that display a nonlinear Langmuir-like behavior.Additionally,we conducted cryo-electron microscopy-to our knowledge,for the first time-on concentrated oligoarginines R9,which,unlike nona-lysines K9,exhibit aggregation in water.These results point to like charge pairing of the guanidinium side chain groups,as corroborated also by MD simulations and free energy calculations.展开更多
Background:Cardiorespiratory fitness(CRF)is a powerful predictor of mortality and chronic disease risk,yet global patterns and determinants of CRF remain poorly defined,particularly in females and underrepresented pop...Background:Cardiorespiratory fitness(CRF)is a powerful predictor of mortality and chronic disease risk,yet global patterns and determinants of CRF remain poorly defined,particularly in females and underrepresented populations.We conducted a systematic review and quantitative synthesis of directly measured peak oxygen uptake(VO_(2peak))internationally and examined its association with human development and gender ine quality.Methods:Studies were eligible if VO_(2peak)was assessed via direct gas analysis during maximal exercise testing,and if the countries had scores for the Human Development Index(HDI)and Gender Inequality Index(GII).Studies were identified through MEDLINE/PubMed,Embase,CINAHL,and Web of Science.Risks of bias were assessed by an adaptation of the Newcastle-Ottawa Scale.Multivariable linear regression models examined associations between VO_(2peak),age,sex,exercise modality,HDI,GII,and study year.Results:Data included 95 studies from 24 countries with HDI and GII scores,comprising 119,435 adults(42%females)with VO_(2peak)assessed via direct gas analysis during maximal exercise testing.The risk of bias was low.VO_(2peak)was positively associated with HDI(β=14.1)and negatively associated with GII(β=-3.6).Slightly stronger associations were observed in females than males(HDI:β=18.9 vs.β=13.9,GII:β=-4.6vs.β=-3.6).Young females in middle-HDI countries had higher VO_(2peak)than those in low-HDI countries(31.2mL/kg/min vs.28.5 mL/kg/min),with limited additional gams in high-HDI contexts.VO_(2peak)decreased with higher gender inequality,with the largest disparities observed in young females between high-and low-GII countries(26.3 mL/kg/min vs.32.8 mL/kg/min).Conclusion:Global variation in CRF is tied to national levels of human development and gender equality.These findings support prioritizing structural and policy-level interventions that address social and gender disparities in physical activity access and health promotion.Studies from countries with lower HDI and information on ethnicity and socioeconomic status will bridge crucial gaps in understanding factors involved in global CRF levels.展开更多
Background： Malignant tumors of the child represent one of the major causes of mortality in children between 1 and 14 years in the West. Young people recovering from childhood cancer may have complications related to...Background： Malignant tumors of the child represent one of the major causes of mortality in children between 1 and 14 years in the West. Young people recovering from childhood cancer may have complications related to the disease itself or treatment. Complications can be somatic, psychic or socio-occupational and familial. The current study aims to know the current status of young children treated in the oncology unit of PHOU （Pediatric Hemato-Oncology Unit）, Rabat Children Hospital is exclusively or jointly between 1978 and 2004. Material and methods： It will be a cross-sectional study conducted among childhood cancer survivors treated between 1978 and 2004 at the Pediatric Oncology Unit of Children＇s Hospital of Rabat. Medical and demographic data will be collected through questionnaires completed by phone or intemet, by the survivor, his parent or his physician. Discussion： To our knowledge, there is no previous study about the fate of Moroccan childhood cancer survivors before our study. Hence, it will be a cross-sectional study to have a quick picture on the situation in Morocco. It would be necessary to establish a follow-up strategy in Morocco.展开更多
Natural peatlands are a source of CH_(4) emission but a sink of CO_(2) and N_(2)O.On the other hand,peatlands drained for agricultural use suppress CH_(4) emission but become a source of CO_(2) and N_(2)O emissions.Dr...Natural peatlands are a source of CH_(4) emission but a sink of CO_(2) and N_(2)O.On the other hand,peatlands drained for agricultural use suppress CH_(4) emission but become a source of CO_(2) and N_(2)O emissions.Drained peatland area accounts for 2% of the world's agricultural land,but its greenhouse gas(GHG)emissions account for 7%of global GHG emissions.Immediately after land clearing,N_(2)O emission significantly increases due to nitrogen(N)fertilization.展开更多
To understand the roles of charcoal and ectomycorrhizal fungi(ECMF)on tree growth,which relates to the rehabilitation of forest ecosystems after forest fires,two experiments were set up in this study,the first was to ...To understand the roles of charcoal and ectomycorrhizal fungi(ECMF)on tree growth,which relates to the rehabilitation of forest ecosystems after forest fires,two experiments were set up in this study,the first was to determine the correct amount of charcoal for Japanese larch(Larix kaempferi Sarg.)seedling growth by applying oak charcoal to basic soil medium at ratios of 1:1,1:2,1:4 and 1:8 by volume.The second experiment investigated the combined effects of four types of charcoal:derived from oak wood,husks of buckwheat,rice and activated charcoal of larch wood,and two types of ECMF:Pt(Pisolithus tinctorius Pers.)and Ec(Pt+Rhizopogon spp.+Laccaria spp.+Scleroderma spp.)on the growth of Japanese larch seedlings.Our results show that growth was significantly stressed by large amounts charcoal applications.There were significant variations among the four types of charcoal on growth.We concluded that the addition of charcoal was the critical factor that influenced larch growth and ECMF formation.Rice charcoal and Ec stimulates the growth and nitrogen uptake of Japanese larch seedlings,thus the most suitable fungus and charcoal for practices is Ec-rice charcoal(1:8 charcoal to basic soil).展开更多
Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implemen...Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implementation,or configuration.To guarantee the durability&robustness of the software,vulnerability identification and fixation have become crucial areas of focus for developers,cybersecurity experts and industries.This paper presents a thorough multi-phase mathematical model for efficient patch management and vulnerability detection.To uniquely model these processes,the model incorporated the notion of the learning phenomenon in describing vulnerability fixation using a logistic learning function.Furthermore,the authors have used numerical methods to approximate the solution of the proposed framework where an analytical solution is difficult to attain.The suggested systematic architecture has been demonstrated through statistical analysis using patch datasets,which offers a solid basis for the research conclusions.According to computational research,learning dynamics improves security response and results in more effective vulnerability management.The suggested model offers a systematic approach to proactive vulnerability mitigation and has important uses in risk assessment,software maintenance,and cybersecurity.This study helps create more robust software systems by increasing patch management effectiveness,which benefits developers,cybersecurity experts,and sectors looking to reduce security threats in a growing digital world.展开更多
The duration of snow cover has shortened in the boreal region,and the amount of seasonal snow decreased.This affects the coupling between soil and air temperatures and may thus lead to colder soil and deeper soil fros...The duration of snow cover has shortened in the boreal region,and the amount of seasonal snow decreased.This affects the coupling between soil and air temperatures and may thus lead to colder soil and deeper soil frost.We prevented snow reaching the forest floor for two winters in mature boreal forest and studied how that affects tree and forest floor processes.The studied species were Scots pine,Norway spruce,silver birch,and a dwarf shrub bilberry.Decreased soil temperature,due to the lack of snow cover,decreased forest floor respiration in winter and spring.Simultaneously,response of respiration to temperature seemed to increase,perhaps due to the exposure of forest floor vegetation to cold air temperature.Indeed,lack of snow cover induced mortality of bilberry,but the remaining ramets grew more in height and their average leaf size was larger likely to compensate for the lost plant biomass.Lack of snow cover also affected tree hydraulics as tree water uptake was decreased in spring,and the start of the sap season delayed in birch.Pine and birch tended to grow less in the snow exclusion treatment(differences not statistically significant),whereas spruce grew more.Coarse root traits,e.g.water content and cellular frost damages,were not affected by the snow exclusion treatment.The results of this case study increase our understanding on the effects of changing snow cover on spring-time tree and forest floor processes in mature boreal forest,but also reveal the need for further studies on mature trees.展开更多
Objective:To examine how emotional labor demands and strategies influence work engagement and occupational identity among Portuguese volunteer firefighters.Methods:Participants completed the Emotional Work Requirement...Objective:To examine how emotional labor demands and strategies influence work engagement and occupational identity among Portuguese volunteer firefighters.Methods:Participants completed the Emotional Work Requirements Scale,Emotional Labour Scale,Utrecht Work Engagement Scale,and Social Identity Scale.Multiple linear regression analyses were conducted to assess the predictive effects of emotional labor demands and strategies on work engagement and occupational identity.Results:Emotional labor explained 11.8%of the variance in work engagement and 10.9%in occupational identity.Positive emotion expression significantly predicted higher work engagement and a stronger occupational identity,while the suppression of negative emotions predicted a weaker occupational identity.Other emotional labor dimensions were not significant.Conclusions:Findings underscore the dual role of emotional labor—as a resource when positive emotions are expressed and as a strain when negative emotions are habitually suppressed.Targeted interventions to promote authentic positive expression and effective emotional processing may enhance firefighters’well-being,strengthen crisis team performance,and benefit the communities they serve.Further research is needed to deepen the understanding of emotional labor in crisis response contexts.展开更多
Immuno-positron emission tomography(immuno-PET)is an innovative medical imaging technique that combines antibodies(Abs)or other immune-targeting molecules with positron-emitting radionuclides.By targeting antigens tha...Immuno-positron emission tomography(immuno-PET)is an innovative medical imaging technique that combines antibodies(Abs)or other immune-targeting molecules with positron-emitting radionuclides.By targeting antigens that are highly expressed in hematologic malignancies,immuno-PET has transformed diagnostic capabilities and enables precise monitoring of therapeutic responses through highly sensitive and specific tumor cell detection.Additionally,it plays a critical role in advancing therapeutic approaches by seamlessly linking diagnostic imaging with personalized treatment strategies.Its non-invasive nature and ability to provide whole-body imaging offer significant advantages over traditional diagnostic methods,especially for detecting minimal residual disease and guiding adaptive therapeutic interventions.In Ab-based immuno-PET,positronemitting radionuclides must have a half-life sufficient for slower pharmacokinetics and blood clearance of Abs.Recent studies have highlighted the advantages of long-lived radionuclides,such as 89Zr,which exhibit low positron energy and enable high sensitivity and resolution,making them particularly effective for tumor visualization and characterization.This review explores the current applications,recent advancements,and potential of immuno-PET for hematologic malignancies,emphasizing its pivotal role in improving patient outcomes and advancing precision medicine.展开更多
Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations b...Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations by formulating a double nano-emulsion(W/O/W)system incorporating macadamia oil and tea tree oil,using homogenization and phase inversion temperature(PIT)techniques.Comprehensive physicochemical charac-terization,including droplet size,polydispersity index(PDI),zeta potential,turbidity,Fourier transform infrared spectroscopy(FTIR),and SEM,was conducted alongside stability assessments under varying pH,temperature,and storage conditions.The optimized nano-emulsions exhibited nanoscale droplet sizes(10-40 nm),low PDI values(indicating high uniformity),and robust stability.Interestingly,the formulation with 2%W/O loading,with a particle size of 11.57 nm and a PDI of 0.04,demonstrated an antioxidant capacity of 4622.62μg ascorbic acid equivalents(AA)/g,which was significantly higher(p<0.05)compared to both natural oils(macadamia oil:20.91μg AA/g,tea tree oil:16.86μg AA/g)and a 10%Vitamin C aqueous solution(592.94μg AA/g).FTIR analysis confirmed the molecular integrity of Vitamin C and its successful encapsulation with macadamia and tea tree oils,while SEM images revealed uniformly spherical and well-dispersed droplets.Moreover,the formulation retained its structural integrity and antioxidant functionality under diverse pH and thermal conditions.These findings underscore the potential of double nano-emulsion systems to overcome the stability challenges of Vitamin C,offering a promising approach to enhance its bioavailability and therapeutic performance in phar-maceutical and cosmetic applications.展开更多
In the contemporary global landscape,understanding the nexus between financial inclusion and natural resource abundance is crucial,especially for resource-rich nations.This study uses diagnostic tests and method of mo...In the contemporary global landscape,understanding the nexus between financial inclusion and natural resource abundance is crucial,especially for resource-rich nations.This study uses diagnostic tests and method of moments quantile regression to examines this interplay across Australia,Brazil,Canada,China,India,Russia,and the United States.We find that achieving financial inclusion is significantly challenging for countries that heavily rely on natural resources.Diversified income sources and equitable wealth distribution are essential to mitigate these challenges.Additionally,we identify a positive correlation between economic development and financial inclusion,highlighting the mutually reinforcing relationship between growth and inclusivity.Our research also reveals a notable link between adopting renewable energy and improving financial inclusion,suggesting that environmental responsibility and financial accessibility are intertwined.Foreign direct investment has nuanced impacts on financial inclusion,adding depth to our understanding.Overall,stable income from natural resources and diversified economic development emerge as key promoters of financial inclusion.These insights advocate for regionally specific policies and lay a solid foundation for future research and informed policymaking that address financial inclusion challenges and advance sustainable development.展开更多
This study focuses on the preparation,and optimization of the nanoemulsions coorporating with pumpkin seed oil,grape seed oil,and grapefruit essential oil using the phase inversion temperature(PIT)technique.The resear...This study focuses on the preparation,and optimization of the nanoemulsions coorporating with pumpkin seed oil,grape seed oil,and grapefruit essential oil using the phase inversion temperature(PIT)technique.The research investigated the impact of surfactant types and concentrations on critical nanoemulsion properties,including droplet size,polydispersity index(PDI),and zeta potential.Using a Box-Behnken Design(BBD)model,the formulation was optimized containing 6.0%plant oils,10.0%Tween 80,2.0%Span 80,and 1.0%lecithin to achieve nano-sized droplets(33.52 nm),with a low PDI(0.205),and a stable zeta potential(15.49 mV).The antioxidant activity,was evaluated through 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging assays,demonstrating its outstanding efficacy.And the optimized nanoemulsion showed a radical-scavenging capacity exceeding 2250μg ascorbic acid equivalents/g,significantly outperforming non-nanoemulsified oils.Stability testing under various environmental conditions highlighted exceptional robustness,with refrigerated samples maintaining structural integrity,minimal particle size growth,and consistent physicochemical properties over a 30-day storage period.The results suggest that the plant oil-based nanoemulsions exhibit strong antioxidant potential,offering a promising natural treatment for their application in cosmeceutical and therapeutic formulations.展开更多
Cyber threat detection is a crucial aspect of contemporary cybersecurity due to the depth and complexity of cyberattacks.It is the identification of malicious activity,unauthorized access,and possible intrusions in ne...Cyber threat detection is a crucial aspect of contemporary cybersecurity due to the depth and complexity of cyberattacks.It is the identification of malicious activity,unauthorized access,and possible intrusions in networks and systems.Modern detection methods employ artificial intelligence and machine learning to study vast amounts of data,learn patterns,and anticipate potential threats.Real-time monitoring and anomaly detection improve the capacity to react to changing threats more rapidly.Cyber threat detection systems aim to reduce false positives and provide complete coverage against the broadest possible attacks.This research advocates for proactive measures and adaptive technologies in defending digital environments.Improvements in detection ability by organizations will assist in safeguarding assets and integrity in operations in this increasingly digital world.This paper draws on the categorization of cyber threat detection methods using hesitant bipolar fuzzy Frank operators.Categorization is a step that is necessary for systematic comparison and assessment of detection methods so that the most suitable method for particular cybersecurity requirements is chosen.Furthermore,this research manages uncertainty and vagueness that exists in decision-making by applying hesitant bipolar fuzzy logic.The importance of the work lies in how it fortifies cybersecurity architectures with a formal method of discovering optimal detection measures and improving responsiveness,resulting in holistic protection against dynamic threats.展开更多
Liver cancer remains one of the leading causes of cancer-related mortality worldwide,with hepatocellular carcinoma representing its most prevalent form.This review provides a comprehensive summary of the key factors d...Liver cancer remains one of the leading causes of cancer-related mortality worldwide,with hepatocellular carcinoma representing its most prevalent form.This review provides a comprehensive summary of the key factors driving the initiation and progression of hepatocellular carcinoma,with a particular emphasis on immune-related mechanisms.Furthermore,we delve into the emerging roles of galectins,particularly galectin-1 and galectin-3,in hepatocellular carcinoma pathobiology,underscoring their potential utility as biomarkers for assessing disease severity and progression.These insights contribute to a better understan-ding of the molecular and immunological underpinnings of hepatocellular carcinoma,paving the way for more targeted therapeutic strategies.展开更多
Regular physical activity is associated with lower adult cancer incidence and mortality,1 with this protective association seemingly independent of important risk factors (high body weight, smoking).2 The potential pr...Regular physical activity is associated with lower adult cancer incidence and mortality,1 with this protective association seemingly independent of important risk factors (high body weight, smoking).2 The potential preventive effect of an active lifestyle was presumed a long time ago by some visionary scientists.展开更多
Sulfur-bearing species are widely utilized to investigate the physical structure of star-forming regions in interstellar media;however,the underlying sulfur chemistry in these environments remains poorly understood.Th...Sulfur-bearing species are widely utilized to investigate the physical structure of star-forming regions in interstellar media;however,the underlying sulfur chemistry in these environments remains poorly understood.Therefore,further studies of S-bearing species are fundamentally important,as they can enhance our understanding of the physical evolution of star-forming regions.This study presents observations of C_(2)S and C_(3)S in L1544,acquired using the Nanshan 26-m radio telescope,along with simulations of their chemical behavior using a one-dimensional physical model.The simulation results reveal significant radial variations in the column densities of C_(2)S and C_(3)S.Additionally,the column densities of both molecules are found to be sensitive to the cosmic ray ionization rate at several radial positions,while variations in the C/O ratio have comparatively minimal impact on L1544.展开更多
文摘Objective:To characterize placental morphologic features in Moroccan women with adverse outcomes,across different clinical contexts,based on the Amsterdam consensus classification.Methods:A prospective analysis was conducted on placentas with umbilical cords collected fresh between March 1,2024 and July 15,2024 from women with adverse pregnancy outcomes.Clinical data(age,parity,gravidity,complications)were retrieved.Macroscopic parameters(weight,dimensions,cord insertion,membranes,lesions)were assessed,followed by systematic sampling.Tissue was processed by standard histology(formalin fixation,paraffin embedding,hematoxylin and eosin staining),and lesions were classified per Amsterdam criteria.Results:16 placentas from patients with adverse pregnancy outcomes were included.The median maternal age was 30 years.Adverse conditions included placental abruption(50%),intrauterine growth restriction(IUGR,38%),intrauterine fetal death(IUFD,31%),pre-eclampsia/eclampsia(19%),premature rupture of membranes(13%),and oligohydramnios(13%).Several placentas were associated with more than one adverse condition.Histopathology revealed maternal vascular malperfusion lesions in 94%,particularly in pre-eclampsia,IUGR,and IUFD.Fetal vascular malperfusion was found in 88%,mainly in IUGR and IUFD.Inflammatory lesions,dominated by acute maternal and fetal responses stage 3(necrotizing chorioamnionitis and funisitis),were primarily linked to IUFD.Conclusions:Placental examination enhances understanding of the pathophysiology underlying adverse pregnancy outcomes,supports diagnostic confirmation,and guides preventive strategies for recurrence.This study highlights the prevalence of maternal vascular malperfusion in Moroccan women and emphasizes the importance of systematic placental histopathology in obstetric care.
基金funded by the Ministry of Health of the Czech Republic—conceptual development of research organization(MMCI,00209805)Czech Science Foundation(No.25-15990S)+1 种基金the project 7D241003 EUREKA EUROSTARS35897,project SALVAGE(P JAC,reg.No.CZ.02.01.01/00/22_008/0004644)—funded by the European Unionby the State Budget of the Czech Republic,and by the LRI project BBMRI.cz(Nos.LM2023033 and CZ.02.1.01/0.0/0.0/16_013/0001674.).
文摘Objectives:Cancer treatment relies heavily on accurate diagnosis and effective monitoring of the disease.These processes often involve invasive procedures,such as colonoscopy,to detect malignant tissues,followed by molecular analyses to determine relevant biomarkers.This study aimed to evaluate the clinical performance of droplet digital PCR(ddPCR)for detecting Kirsten Rat Sarcoma Viral Proto-Oncogene(KRAS),Neuroblastoma RAS Viral Oncogene Homolog(NRAS),and B-Raf Murine Sarcoma Viral Oncogene Homolog B(BRAF)mutations in circulating tumor DNA(ctDNA)from colorectal cancer patients using liquid biopsy.Methods:ctDNA was isolated from colorectal cancer(CRC)patients(n=110)and analyzed for KRAS,BRAF,and NRAS mutations.The ctDNA obtained through liquid biopsy was analyzed using ddPCR,and the findings were compared with sequencing data from tumor DNA archived in formalin-fixed paraffin-embedded(FFPE)blocks.Results:For KRAS mutations,ddPCR achieved a sensitivity of 72.0%and a specificity of 71.4%.However,when pooling all target mutations(KRAS,NRAS and BRAF),the overall sensitivity and specificity were lower,at 48.3%and 51.1%,respectively.Conclusion:The results of this study indicate that the ddPCR analysis of ctDNA may provide complementary information for the molecular diagnosis of CRC patients.
基金supported by the Czech Science Foundation(projects 24-11371S to TM and 2306840S to MAL)Masaryk University Grant Agency(project MUNI/R/1268/2022 to TM)+1 种基金part of a long-term research project of the Czech Academy of Sciences,Institute of Botany(RVO 67985939)provided by the ELIXIR-CZ project(LM2023055),part of the international ELIXIR infrastructure。
文摘The genus Boechera(Brassicaceae)serves as a model system for studying apomictic reproduction and ecological adaptations,with most species occurring in North America.The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments.One such species,B.calcarea,has been described from the Chandalaz Mountains in northeastern Asia(Russia).This study aimed to investigate the genome structure and evolutionary history of B.calcarea.However,our analyses reveal that the species does not belong to Boechera.Instead,an integrative approach combining cytogenetic,phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae.The diploid Parryodes calcarea(2n=16)exhibits Arabideae-specific chromosomal signatures,including multiple centromere repositionings.These findings clarify the misclassification of P.calcarea as Boechera,leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World.This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe,the Arabideae.
基金support from the project“National Institute of Virology and Bacteriology(Program EXCELES,ID Project No.LX22NPO5103)Funded by the European Union-Next Generation EU".D.B.also acknowledges VSB-Technical University of Ostrava,IT4Innovations National Supercomputing Center,Czech Republic,for awarding this project access to the LUMI supercomputer,owned by the EuroHPC Joint Undertaking,hosted by CSC(Finland)and the LUMI consortium through the Ministry of Education,Youth and Sports of the Czech Republic through the e-INFRA CZ(Grant ID:90254)+4 种基金project OPEN-35-3.M.V.and J.H.acknowledge the Czech Science Foundation for support via grant number 25-16117S and the project"The Energy Conversion and Storage"funded as project No.CZ.02.01.01/00/22_008/0004617 by Programme Johannes Amos Comeniuscall Excellent Research.M.V.also acknowledges support by the Ministry of Education,Youth and Sports of the Czech Republic through the e-INFRA CZ(ID:90254)Project OPEN-30-53.P.J.acknowledges support from the European Research Council via an ERC Advanced Grant no.101095957The authors would like to acknowledge the contribution of COST Action CA21169,supported by COST(European Cooperation in Science and Technology).
文摘Like-charge pairing is a physical manifestation of the unique solvation properties of certain ion pairs in water.Water's high dielectric constant and related charge screening capability significantly influence the interaction between like-charged ions,with the possibility to transform it-in exceptional cases when noncovalent interactions are involved-from repulsion to attraction.Guanidinium cations(Gdm^(+))represent a quintessential example of such like-charge pairing due to their specific geometry and electronic structure.In this work,we present experimental validation and quantification of Gdm^(+)-Gdm contact ion pairing in water utilizing nuclear magnetic resonance(NMR)spectroscopy complemented by molecular dynamics(MD)simulations and density functional theory(DFT)calculations.The observed Gdm^(+)-Gdm^(+)interaction is attractive albeit weak-about 0.5 kJ·mol^(-1)-which aligns with theoretical estimation from MD simulations.We contrast the behavior of Gdm^(+) with that of NH_(4)^(+) cations,which exhibit no contact ion pairing in water.DFT calculations predict that the NMR chemical shift of Gdm^(+) dimers is different than that of monomers,in agreement with NMR titration curves that display a nonlinear Langmuir-like behavior.Additionally,we conducted cryo-electron microscopy-to our knowledge,for the first time-on concentrated oligoarginines R9,which,unlike nona-lysines K9,exhibit aggregation in water.These results point to like charge pairing of the guanidinium side chain groups,as corroborated also by MD simulations and free energy calculations.
基金NJP holds a Future Leader Award from the Novo Nordisk Foundation and European Foundation for the Study of Diabetes(NNF/EFSD NNF21SA0072747)a grant from the Diabetes Wellness Network Sverige(PG21-6524)+5 种基金supported by the Swedish Research Council(2015-00165)the European Research Council(ERC-2023-Ad G 101142093)a Wallenberg Scholars Award from the Knut and Alice Wallenberg Foundation(KAW 2023.0312)the Swedish Research Council for Sport Science(P2023-0093)The Novo Nordisk Foundation Center for Basic Metabolic Research is an independent research center at the Faculty of Health and Medical Sciences,University of Copenhagen,Denmark partially funded by an unrestricted donation from the Novo Nordisk Foundation(NNF18CC0034900,NNF23SA0084103)supported by a postdoctoral fellowship from the Strategic Research Program in Diabetes at Karolinska Institutet。
文摘Background:Cardiorespiratory fitness(CRF)is a powerful predictor of mortality and chronic disease risk,yet global patterns and determinants of CRF remain poorly defined,particularly in females and underrepresented populations.We conducted a systematic review and quantitative synthesis of directly measured peak oxygen uptake(VO_(2peak))internationally and examined its association with human development and gender ine quality.Methods:Studies were eligible if VO_(2peak)was assessed via direct gas analysis during maximal exercise testing,and if the countries had scores for the Human Development Index(HDI)and Gender Inequality Index(GII).Studies were identified through MEDLINE/PubMed,Embase,CINAHL,and Web of Science.Risks of bias were assessed by an adaptation of the Newcastle-Ottawa Scale.Multivariable linear regression models examined associations between VO_(2peak),age,sex,exercise modality,HDI,GII,and study year.Results:Data included 95 studies from 24 countries with HDI and GII scores,comprising 119,435 adults(42%females)with VO_(2peak)assessed via direct gas analysis during maximal exercise testing.The risk of bias was low.VO_(2peak)was positively associated with HDI(β=14.1)and negatively associated with GII(β=-3.6).Slightly stronger associations were observed in females than males(HDI:β=18.9 vs.β=13.9,GII:β=-4.6vs.β=-3.6).Young females in middle-HDI countries had higher VO_(2peak)than those in low-HDI countries(31.2mL/kg/min vs.28.5 mL/kg/min),with limited additional gams in high-HDI contexts.VO_(2peak)decreased with higher gender inequality,with the largest disparities observed in young females between high-and low-GII countries(26.3 mL/kg/min vs.32.8 mL/kg/min).Conclusion:Global variation in CRF is tied to national levels of human development and gender equality.These findings support prioritizing structural and policy-level interventions that address social and gender disparities in physical activity access and health promotion.Studies from countries with lower HDI and information on ethnicity and socioeconomic status will bridge crucial gaps in understanding factors involved in global CRF levels.
文摘Background： Malignant tumors of the child represent one of the major causes of mortality in children between 1 and 14 years in the West. Young people recovering from childhood cancer may have complications related to the disease itself or treatment. Complications can be somatic, psychic or socio-occupational and familial. The current study aims to know the current status of young children treated in the oncology unit of PHOU （Pediatric Hemato-Oncology Unit）, Rabat Children Hospital is exclusively or jointly between 1978 and 2004. Material and methods： It will be a cross-sectional study conducted among childhood cancer survivors treated between 1978 and 2004 at the Pediatric Oncology Unit of Children＇s Hospital of Rabat. Medical and demographic data will be collected through questionnaires completed by phone or intemet, by the survivor, his parent or his physician. Discussion： To our knowledge, there is no previous study about the fate of Moroccan childhood cancer survivors before our study. Hence, it will be a cross-sectional study to have a quick picture on the situation in Morocco. It would be necessary to establish a follow-up strategy in Morocco.
文摘Natural peatlands are a source of CH_(4) emission but a sink of CO_(2) and N_(2)O.On the other hand,peatlands drained for agricultural use suppress CH_(4) emission but become a source of CO_(2) and N_(2)O emissions.Drained peatland area accounts for 2% of the world's agricultural land,but its greenhouse gas(GHG)emissions account for 7%of global GHG emissions.Immediately after land clearing,N_(2)O emission significantly increases due to nitrogen(N)fertilization.
基金supported by the National Key Research and Development Program of China(2022 YFF 1303201)the Japan Society for the Promotion of Science.
文摘To understand the roles of charcoal and ectomycorrhizal fungi(ECMF)on tree growth,which relates to the rehabilitation of forest ecosystems after forest fires,two experiments were set up in this study,the first was to determine the correct amount of charcoal for Japanese larch(Larix kaempferi Sarg.)seedling growth by applying oak charcoal to basic soil medium at ratios of 1:1,1:2,1:4 and 1:8 by volume.The second experiment investigated the combined effects of four types of charcoal:derived from oak wood,husks of buckwheat,rice and activated charcoal of larch wood,and two types of ECMF:Pt(Pisolithus tinctorius Pers.)and Ec(Pt+Rhizopogon spp.+Laccaria spp.+Scleroderma spp.)on the growth of Japanese larch seedlings.Our results show that growth was significantly stressed by large amounts charcoal applications.There were significant variations among the four types of charcoal on growth.We concluded that the addition of charcoal was the critical factor that influenced larch growth and ECMF formation.Rice charcoal and Ec stimulates the growth and nitrogen uptake of Japanese larch seedlings,thus the most suitable fungus and charcoal for practices is Ec-rice charcoal(1:8 charcoal to basic soil).
基金supported by grants received by the first author and third author from the Institute of Eminence,Delhi University,Delhi,India,as part of the Faculty Research Program via Ref.No./IoE/2024-25/12/FRP.
文摘Software systems are vulnerable to security breaches as they expand in complexity and functionality.The confidentiality,integrity,and availability of data are gravely threatened by flaws in a system’s design,implementation,or configuration.To guarantee the durability&robustness of the software,vulnerability identification and fixation have become crucial areas of focus for developers,cybersecurity experts and industries.This paper presents a thorough multi-phase mathematical model for efficient patch management and vulnerability detection.To uniquely model these processes,the model incorporated the notion of the learning phenomenon in describing vulnerability fixation using a logistic learning function.Furthermore,the authors have used numerical methods to approximate the solution of the proposed framework where an analytical solution is difficult to attain.The suggested systematic architecture has been demonstrated through statistical analysis using patch datasets,which offers a solid basis for the research conclusions.According to computational research,learning dynamics improves security response and results in more effective vulnerability management.The suggested model offers a systematic approach to proactive vulnerability mitigation and has important uses in risk assessment,software maintenance,and cybersecurity.This study helps create more robust software systems by increasing patch management effectiveness,which benefits developers,cybersecurity experts,and sectors looking to reduce security threats in a growing digital world.
基金supported by the European Union-Next Generation EU instrument and the Research Council of Finland funding(grants 323843,347782,353218,355142,and 357902)INAR RI research infrastructure。
文摘The duration of snow cover has shortened in the boreal region,and the amount of seasonal snow decreased.This affects the coupling between soil and air temperatures and may thus lead to colder soil and deeper soil frost.We prevented snow reaching the forest floor for two winters in mature boreal forest and studied how that affects tree and forest floor processes.The studied species were Scots pine,Norway spruce,silver birch,and a dwarf shrub bilberry.Decreased soil temperature,due to the lack of snow cover,decreased forest floor respiration in winter and spring.Simultaneously,response of respiration to temperature seemed to increase,perhaps due to the exposure of forest floor vegetation to cold air temperature.Indeed,lack of snow cover induced mortality of bilberry,but the remaining ramets grew more in height and their average leaf size was larger likely to compensate for the lost plant biomass.Lack of snow cover also affected tree hydraulics as tree water uptake was decreased in spring,and the start of the sap season delayed in birch.Pine and birch tended to grow less in the snow exclusion treatment(differences not statistically significant),whereas spruce grew more.Coarse root traits,e.g.water content and cellular frost damages,were not affected by the snow exclusion treatment.The results of this case study increase our understanding on the effects of changing snow cover on spring-time tree and forest floor processes in mature boreal forest,but also reveal the need for further studies on mature trees.
基金funded by national funds through FCT-Fundação para a Ciência e a TecnologiaI.P.(Foundation for Science and Technology,I.P.) under project UIDB/05037/2020.
文摘Objective:To examine how emotional labor demands and strategies influence work engagement and occupational identity among Portuguese volunteer firefighters.Methods:Participants completed the Emotional Work Requirements Scale,Emotional Labour Scale,Utrecht Work Engagement Scale,and Social Identity Scale.Multiple linear regression analyses were conducted to assess the predictive effects of emotional labor demands and strategies on work engagement and occupational identity.Results:Emotional labor explained 11.8%of the variance in work engagement and 10.9%in occupational identity.Positive emotion expression significantly predicted higher work engagement and a stronger occupational identity,while the suppression of negative emotions predicted a weaker occupational identity.Other emotional labor dimensions were not significant.Conclusions:Findings underscore the dual role of emotional labor—as a resource when positive emotions are expressed and as a strain when negative emotions are habitually suppressed.Targeted interventions to promote authentic positive expression and effective emotional processing may enhance firefighters’well-being,strengthen crisis team performance,and benefit the communities they serve.Further research is needed to deepen the understanding of emotional labor in crisis response contexts.
文摘Immuno-positron emission tomography(immuno-PET)is an innovative medical imaging technique that combines antibodies(Abs)or other immune-targeting molecules with positron-emitting radionuclides.By targeting antigens that are highly expressed in hematologic malignancies,immuno-PET has transformed diagnostic capabilities and enables precise monitoring of therapeutic responses through highly sensitive and specific tumor cell detection.Additionally,it plays a critical role in advancing therapeutic approaches by seamlessly linking diagnostic imaging with personalized treatment strategies.Its non-invasive nature and ability to provide whole-body imaging offer significant advantages over traditional diagnostic methods,especially for detecting minimal residual disease and guiding adaptive therapeutic interventions.In Ab-based immuno-PET,positronemitting radionuclides must have a half-life sufficient for slower pharmacokinetics and blood clearance of Abs.Recent studies have highlighted the advantages of long-lived radionuclides,such as 89Zr,which exhibit low positron energy and enable high sensitivity and resolution,making them particularly effective for tumor visualization and characterization.This review explores the current applications,recent advancements,and potential of immuno-PET for hematologic malignancies,emphasizing its pivotal role in improving patient outcomes and advancing precision medicine.
基金Ho Chi Minh City University of Technology(HCMUT),VNU-HCM for supporting this study.
文摘Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations by formulating a double nano-emulsion(W/O/W)system incorporating macadamia oil and tea tree oil,using homogenization and phase inversion temperature(PIT)techniques.Comprehensive physicochemical charac-terization,including droplet size,polydispersity index(PDI),zeta potential,turbidity,Fourier transform infrared spectroscopy(FTIR),and SEM,was conducted alongside stability assessments under varying pH,temperature,and storage conditions.The optimized nano-emulsions exhibited nanoscale droplet sizes(10-40 nm),low PDI values(indicating high uniformity),and robust stability.Interestingly,the formulation with 2%W/O loading,with a particle size of 11.57 nm and a PDI of 0.04,demonstrated an antioxidant capacity of 4622.62μg ascorbic acid equivalents(AA)/g,which was significantly higher(p<0.05)compared to both natural oils(macadamia oil:20.91μg AA/g,tea tree oil:16.86μg AA/g)and a 10%Vitamin C aqueous solution(592.94μg AA/g).FTIR analysis confirmed the molecular integrity of Vitamin C and its successful encapsulation with macadamia and tea tree oils,while SEM images revealed uniformly spherical and well-dispersed droplets.Moreover,the formulation retained its structural integrity and antioxidant functionality under diverse pH and thermal conditions.These findings underscore the potential of double nano-emulsion systems to overcome the stability challenges of Vitamin C,offering a promising approach to enhance its bioavailability and therapeutic performance in phar-maceutical and cosmetic applications.
基金supported by the grant from National Social Science Fund of China(23BJY085).
文摘In the contemporary global landscape,understanding the nexus between financial inclusion and natural resource abundance is crucial,especially for resource-rich nations.This study uses diagnostic tests and method of moments quantile regression to examines this interplay across Australia,Brazil,Canada,China,India,Russia,and the United States.We find that achieving financial inclusion is significantly challenging for countries that heavily rely on natural resources.Diversified income sources and equitable wealth distribution are essential to mitigate these challenges.Additionally,we identify a positive correlation between economic development and financial inclusion,highlighting the mutually reinforcing relationship between growth and inclusivity.Our research also reveals a notable link between adopting renewable energy and improving financial inclusion,suggesting that environmental responsibility and financial accessibility are intertwined.Foreign direct investment has nuanced impacts on financial inclusion,adding depth to our understanding.Overall,stable income from natural resources and diversified economic development emerge as key promoters of financial inclusion.These insights advocate for regionally specific policies and lay a solid foundation for future research and informed policymaking that address financial inclusion challenges and advance sustainable development.
基金Ho Chi Minh City University of Technology(HCMUT),VNU-HCM,for supporting this study.
文摘This study focuses on the preparation,and optimization of the nanoemulsions coorporating with pumpkin seed oil,grape seed oil,and grapefruit essential oil using the phase inversion temperature(PIT)technique.The research investigated the impact of surfactant types and concentrations on critical nanoemulsion properties,including droplet size,polydispersity index(PDI),and zeta potential.Using a Box-Behnken Design(BBD)model,the formulation was optimized containing 6.0%plant oils,10.0%Tween 80,2.0%Span 80,and 1.0%lecithin to achieve nano-sized droplets(33.52 nm),with a low PDI(0.205),and a stable zeta potential(15.49 mV).The antioxidant activity,was evaluated through 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging assays,demonstrating its outstanding efficacy.And the optimized nanoemulsion showed a radical-scavenging capacity exceeding 2250μg ascorbic acid equivalents/g,significantly outperforming non-nanoemulsified oils.Stability testing under various environmental conditions highlighted exceptional robustness,with refrigerated samples maintaining structural integrity,minimal particle size growth,and consistent physicochemical properties over a 30-day storage period.The results suggest that the plant oil-based nanoemulsions exhibit strong antioxidant potential,offering a promising natural treatment for their application in cosmeceutical and therapeutic formulations.
基金funded by Ongoing Research Funding program(ORF-2025-749),King Saud University,Riyadh,Saudi Arabia.
文摘Cyber threat detection is a crucial aspect of contemporary cybersecurity due to the depth and complexity of cyberattacks.It is the identification of malicious activity,unauthorized access,and possible intrusions in networks and systems.Modern detection methods employ artificial intelligence and machine learning to study vast amounts of data,learn patterns,and anticipate potential threats.Real-time monitoring and anomaly detection improve the capacity to react to changing threats more rapidly.Cyber threat detection systems aim to reduce false positives and provide complete coverage against the broadest possible attacks.This research advocates for proactive measures and adaptive technologies in defending digital environments.Improvements in detection ability by organizations will assist in safeguarding assets and integrity in operations in this increasingly digital world.This paper draws on the categorization of cyber threat detection methods using hesitant bipolar fuzzy Frank operators.Categorization is a step that is necessary for systematic comparison and assessment of detection methods so that the most suitable method for particular cybersecurity requirements is chosen.Furthermore,this research manages uncertainty and vagueness that exists in decision-making by applying hesitant bipolar fuzzy logic.The importance of the work lies in how it fortifies cybersecurity architectures with a formal method of discovering optimal detection measures and improving responsiveness,resulting in holistic protection against dynamic threats.
文摘Liver cancer remains one of the leading causes of cancer-related mortality worldwide,with hepatocellular carcinoma representing its most prevalent form.This review provides a comprehensive summary of the key factors driving the initiation and progression of hepatocellular carcinoma,with a particular emphasis on immune-related mechanisms.Furthermore,we delve into the emerging roles of galectins,particularly galectin-1 and galectin-3,in hepatocellular carcinoma pathobiology,underscoring their potential utility as biomarkers for assessing disease severity and progression.These insights contribute to a better understan-ding of the molecular and immunological underpinnings of hepatocellular carcinoma,paving the way for more targeted therapeutic strategies.
基金supported in part by NIH Grant No. U01 TR002004 (REACH project)Research by TP and CFL is funded by the Spanish Ministry of Science and Innovation and (Fondo de Investigaciones Sanitarias, ISCIII)+3 种基金Fondos FEDER (grant numbers PI22/00201 (TP)PI20/00645 and PI23/00396 (CF-L))Research by CFL is also funded by ISCIII (FORT23/00023)Research by AL and CF-L is funded by Wereld Kanker Onderzoek Fonds (WKOF) as part of the World Cancer Research Fund International grant programme (IIG_FULL_2021_007).
文摘Regular physical activity is associated with lower adult cancer incidence and mortality,1 with this protective association seemingly independent of important risk factors (high body weight, smoking).2 The potential preventive effect of an active lifestyle was presumed a long time ago by some visionary scientists.
基金the support from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(Grant No.2024D01E37)the National Science Foundation of China(Grant No.12473025)+5 种基金funded by the National Natural Science Foundation of China(Grant Nos.12373026,12203091,12173075,and 11973076)the Xinjiang Tianchi Talent Program(2024)the support from the Xinjiang Tianchi Talent Program(2023)the Shanghai Natural Science Foundation(Grant No.22ZR1421400)the Natural Science Foundation of Xinjiang Uygur Autonomous Region(Grant No.2022D01A156)partially funded by the Regional Collaborative Innovation Project of Xin jiang Uyghur Autonomous Region(Grant No.2022E01050)。
文摘Sulfur-bearing species are widely utilized to investigate the physical structure of star-forming regions in interstellar media;however,the underlying sulfur chemistry in these environments remains poorly understood.Therefore,further studies of S-bearing species are fundamentally important,as they can enhance our understanding of the physical evolution of star-forming regions.This study presents observations of C_(2)S and C_(3)S in L1544,acquired using the Nanshan 26-m radio telescope,along with simulations of their chemical behavior using a one-dimensional physical model.The simulation results reveal significant radial variations in the column densities of C_(2)S and C_(3)S.Additionally,the column densities of both molecules are found to be sensitive to the cosmic ray ionization rate at several radial positions,while variations in the C/O ratio have comparatively minimal impact on L1544.
