Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells ...Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).展开更多
AIM: To study the effects of elemene (Ele) on proliferation and cell cycle of human lens epithelial cells 63 (HLE-B3) and the mechanisms of its signal transduction. METHODS: Recombinant human basic fibroblast growth f...AIM: To study the effects of elemene (Ele) on proliferation and cell cycle of human lens epithelial cells 63 (HLE-B3) and the mechanisms of its signal transduction. METHODS: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 80mg/L Ele for 24 hours. The inhibitory effects of Ele on the proliferation of HLE-B3 cells were evaluated by MU method. The effect of Ele on HLE-B3 cell cycle was analyzed by flow cytometry (FCM). The expressions of protein kinase A (PKA) and protein kinase G (PKG) of HLE-B3 were also analyzed by FCM. RESULTS: Ele altered the cell cycle of HLE-B3 and effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. Ele up-regulated PKA and down-regulated the expression of PKG in HLE-B3 cell. CONCLUSION: Ele inhibits HLE-B3 proliferation, making it an attractive potential agent in regimens to treat after-cataracts.展开更多
AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H...AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H2 O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. · METHODS: HLE-B3 cells were treated with H2O2(300μmol/L),β-estuarial(E2; 10-8mol/L) and H2 O2,ECR(10-6mol/L) and H2 O2,or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS). The mass/charge(M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. ·RESULTS: H2O2 up-regulated expression of two protein spots(with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage,the expression of one protein spot(M/Z 6 532) was down-regulated. In contrast,ECR downregulated both of protein spots(M/Z 6 532 and 6 809). · CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2 O2.展开更多
Objective： To investigate the protective effects of the natural medicinal monomer isopsoralen （ISR） with estrogenic activity against oxidative damage in human lens epithelial cells B3 （HLE-B3） caused by hydrogen ...Objective： To investigate the protective effects of the natural medicinal monomer isopsoralen （ISR） with estrogenic activity against oxidative damage in human lens epithelial cells B3 （HLE-B3） caused by hydrogen peroxide （H2O2） and to pursue the possible mitochondrial proteomic regularity of the protective effects. Methods： HLE-B3 cells were treated with H2O2 （300 μMol/L）, β-estradiol （E2：10^-8mol/L） and H2O2, ISR （10^-5 mol/L） and H2O2, or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surface- enhanced laser desorpUon ionization time of flight mass spectrometry （SELDI-TOF-MS）. The mass/charge （m/z） ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. Results： H2O2 up-regulated the expressions of two protein spots （with m/z of 6532 and 6809）. E2 mitigated the oxidative damage, and the expression of one protein spot （m/z 6532） was down-regulated. In contrast, ISR down-regulated both of protein spots （m/z 6532 and 6809）. Conclusions： ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.展开更多
ABSTRACT Objective： To develop a reliable method to assess the stability of Xinyue Capsules （心悦胶囊） containing Panax quinquefolius saponins according to European quality standards. Methods： An efficient high-pe...ABSTRACT Objective： To develop a reliable method to assess the stability of Xinyue Capsules （心悦胶囊） containing Panax quinquefolius saponins according to European quality standards. Methods： An efficient high-performance liquid chromatography ultraviolet （HPLC-UV） method was established to analyse six main ginsenosides （Rb1, Rb2, Rc, Rd, Re and Rg1） in six different batches （120 capsules/batch） from the same lot of Xinyue Capsules and in one batch measured six times within one day. The six ginsenosides were separated on a Hypersil BDS-C18 column （3 μm, 100 mm × 3 mm） at a flow rate of 0.5 mL/min. Gradient elution was performed using a mobile phase gradient of acetonitrile-water modified with 0.01% formic acid. The HPLC chromatograms were analyzed with ＂LC data comparison＂ using Lab Solutions software. Results： The HPLC peaks were identified by comparing their retention times （Rg1： 23.44 min, Re： 23.77 rain, Rb1： 35.24 min, Rc： 36.18 min, Rb2：38.55 min and Rd： 40.88 min） with those of the standards under the same chromatographic conditions, which showed similar results among the samples of six different batches and among the samples from one batch detected six times within one day. Conclusions： Xinyue Capsules have good drug intra-day consistency at room temperature and exhibit a consistent quality between different batches. This study established a reliable method to assess the stability of Xinyue Capsules, which is suitable for further qualitative analysis and may assist in promoting the safe and effective use of Chinese herbal medicine.展开更多
基金Project(No.wzzb0605) supported by the Traditional Chinese Medicine Research Fund of the Department of Health of Fujian Province,China
文摘Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).
基金Youth Foundation of Fujian Scientific Committee, China (No.2008F3050)
文摘AIM: To study the effects of elemene (Ele) on proliferation and cell cycle of human lens epithelial cells 63 (HLE-B3) and the mechanisms of its signal transduction. METHODS: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 80mg/L Ele for 24 hours. The inhibitory effects of Ele on the proliferation of HLE-B3 cells were evaluated by MU method. The effect of Ele on HLE-B3 cell cycle was analyzed by flow cytometry (FCM). The expressions of protein kinase A (PKA) and protein kinase G (PKG) of HLE-B3 were also analyzed by FCM. RESULTS: Ele altered the cell cycle of HLE-B3 and effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. Ele up-regulated PKA and down-regulated the expression of PKG in HLE-B3 cell. CONCLUSION: Ele inhibits HLE-B3 proliferation, making it an attractive potential agent in regimens to treat after-cataracts.
基金Supported by Fujian Province Health Department Fund(No.2009-1-30)Fujian Province Department of Education Issues(No.JA10176)
文摘AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H2 O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. · METHODS: HLE-B3 cells were treated with H2O2(300μmol/L),β-estuarial(E2; 10-8mol/L) and H2 O2,ECR(10-6mol/L) and H2 O2,or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS). The mass/charge(M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. ·RESULTS: H2O2 up-regulated expression of two protein spots(with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage,the expression of one protein spot(M/Z 6 532) was down-regulated. In contrast,ECR downregulated both of protein spots(M/Z 6 532 and 6 809). · CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2 O2.
基金Supported by the Foundation of Fujian Province Health Department Fund(No.2009-1-30)Fujian Provincie Department of Education Issues(No.JA10176)
文摘Objective： To investigate the protective effects of the natural medicinal monomer isopsoralen （ISR） with estrogenic activity against oxidative damage in human lens epithelial cells B3 （HLE-B3） caused by hydrogen peroxide （H2O2） and to pursue the possible mitochondrial proteomic regularity of the protective effects. Methods： HLE-B3 cells were treated with H2O2 （300 μMol/L）, β-estradiol （E2：10^-8mol/L） and H2O2, ISR （10^-5 mol/L） and H2O2, or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surface- enhanced laser desorpUon ionization time of flight mass spectrometry （SELDI-TOF-MS）. The mass/charge （m/z） ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. Results： H2O2 up-regulated the expressions of two protein spots （with m/z of 6532 and 6809）. E2 mitigated the oxidative damage, and the expression of one protein spot （m/z 6532） was down-regulated. In contrast, ISR down-regulated both of protein spots （m/z 6532 and 6809）. Conclusions： ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.
基金Supported by the National Natural Scientific Fund of China(No.81273933,81102722)the International Science and Technology Cooperative Project from the Ministry of Science and Technology of China(No.2010DFA31690)the Austrian Federal Ministry of Science and Research and the Austrian Federal Ministry of Health(No.GZBMWF-402.000/3-Ⅳ6b/2008)
文摘ABSTRACT Objective： To develop a reliable method to assess the stability of Xinyue Capsules （心悦胶囊） containing Panax quinquefolius saponins according to European quality standards. Methods： An efficient high-performance liquid chromatography ultraviolet （HPLC-UV） method was established to analyse six main ginsenosides （Rb1, Rb2, Rc, Rd, Re and Rg1） in six different batches （120 capsules/batch） from the same lot of Xinyue Capsules and in one batch measured six times within one day. The six ginsenosides were separated on a Hypersil BDS-C18 column （3 μm, 100 mm × 3 mm） at a flow rate of 0.5 mL/min. Gradient elution was performed using a mobile phase gradient of acetonitrile-water modified with 0.01% formic acid. The HPLC chromatograms were analyzed with ＂LC data comparison＂ using Lab Solutions software. Results： The HPLC peaks were identified by comparing their retention times （Rg1： 23.44 min, Re： 23.77 rain, Rb1： 35.24 min, Rc： 36.18 min, Rb2：38.55 min and Rd： 40.88 min） with those of the standards under the same chromatographic conditions, which showed similar results among the samples of six different batches and among the samples from one batch detected six times within one day. Conclusions： Xinyue Capsules have good drug intra-day consistency at room temperature and exhibit a consistent quality between different batches. This study established a reliable method to assess the stability of Xinyue Capsules, which is suitable for further qualitative analysis and may assist in promoting the safe and effective use of Chinese herbal medicine.
