Flexible sensors are promising candidates in personalized healthcare,while desired sensors that allow implantation for biomedical applications with optimal sensing and favorable biological properties remain challenges...Flexible sensors are promising candidates in personalized healthcare,while desired sensors that allow implantation for biomedical applications with optimal sensing and favorable biological properties remain challenges.Here,a multifunctional hydrogel sensor was developed with gallic acid-modified chitosan(CSGA)and 3-carboxyphenylboronic acid-modified chitosan(CSPBA)by encapsulating Agdecorated polydopamine(Ag@PDA)nanoparticles,namely Ag@PDA-(CSPBA/CSGA).The optimized hydrogel sensor showed desired sensitivity(gauge factor=2.49),a rapid response/recovery time of 263 ms and good durability.Due to the presence of abundant reactive groups within Ag@PDA-(CSPBA/CSGA),the hydrogel sensor exhibited a comprehensive performance of self-healing,tissue adhesiveness,antioxidative activity,and antibacterial effects against Escherichia coli(92.76%)and Staphylococcus aureus(98.08%).Moreover,the hydrogel sensor could be utilized as a wound dressing,facilitating accelerated wound closure and tissue regeneration.Both subtle activities and large-scale movements could be monitored and distinguished by the hydrogel sensor.This study provides a promising epidermal sensor that offers multifunctionality for health monitoring and wound management.展开更多
The poor prognosis of triple-negative breast cancer(TNBC)results from its high metastasis,whereas inflammation accompanied by excessive reactive oxygen species(ROS)is prone to aggravate tumor metastasis.Although photo...The poor prognosis of triple-negative breast cancer(TNBC)results from its high metastasis,whereas inflammation accompanied by excessive reactive oxygen species(ROS)is prone to aggravate tumor metastasis.Although photothermal therapy(PTT)has extremely high therapeutic efficiency,the crafty tumor cells allow an increase in the expression of heat shock proteins(HSPs)to limit its effect,and PTT-induced inflammation is also thought to be a potential trigger for tumor metastasis.Herein,myricetin,iron ions,and polyvinylpyrrolidone were utilized to develop nanomedicines by self-assembly strategy for the treatment of metastatic TNBC.The nanomedicines with marvelous water solubility and dispersion can inhibit glucose transporter 1 and interfere with mitochondrial function to block the energy supply of tumor cells,achieving starvation therapy on TNBC cells.Nanomedicines with excellent photothermal conversion properties allow down-regulating the expression of HSPs to enhance the effect of PTT.Interestingly,the broad spectrum of ROS scavenging ability of nanomedicines successfully attenuates PTT-induced inflammation as well as influences hypoxia-inducible factors-1α/3-phosphoinositide-dependent protein kinase 1 related pathway through glycometabolism inhibition to reduce tumor cell metastasis.Moreover,the nanomedicines have negligible side effects and good clinical application prospects,which provides a valuable paradigm for the treatment of metastatic TNBC through glycometabolism interference,anti-inflammation,starvation,and photothermal synergistic therapy.展开更多
Objective： To explore brain activations associated with electroacupuncture simulation at Tongli（HT 5） and its comparison with brain activations during picture-naming task. Methods： Twenty healthy subjects were enr...Objective： To explore brain activations associated with electroacupuncture simulation at Tongli（HT 5） and its comparison with brain activations during picture-naming task. Methods： Twenty healthy subjects were enrolled in this study. Half of them received electroacupuncture stimulation at HT 5（ACUP group） and the other half of them received stimulation at a nonmeridian sham acupoint（SHAM group）. All subjects performed picture-naming task. Each subject finished two runs of functional magnetic resonance imaging examinations in one session and picture-naming task was performed before electroacupuncture stimulation. Subjective brain activations were obtained using generalized linear model and inter-group analyses were performed after that. Results： The electroacupuncture stimulation at HT 5 induced significant brain activations in both the anterior and posterior language regions, including the left inferior frontal gyrus, which was in consistent with activations induced during picture-naming task. Group analysis showed a tendency of increased activation of ACUP group in left inferior frontal gyrus compared with SHAM group（P〈0.05 FDR corrected）. Conclusions： Electroacupuncture treatment at the acupoint HT 5 has modulation effect on typical language-implicated brain regions in healthy subjects, which provides supporting evidence for beneficial effects of needling at HT 5 for recovery of language function in aphasia.展开更多
In recent years,the collection and monitoring of human physiological signals have garnered increasing attention due to their wide-ranging applications in healthcare,human-machine interaction,sports,and other fields.Ho...In recent years,the collection and monitoring of human physiological signals have garnered increasing attention due to their wide-ranging applications in healthcare,human-machine interaction,sports,and other fields.However,the continuous fabrication of flexible gel fiber electrodes with high mechanical performance,high conductivity,and durability for extreme environments using a simple,efficient,and universal strategy remains challenging for physiological signal acquisition.Here,we have employed a strategy of solvent replacement and multi-level hydrogen bond enhancement to construct eutectogel fibers with continuous solid-liquid structure,achieving continuous production of fibers with high strength,high conductivity,and low-temperature resistance.In the fiber,PVA serves as the solid-state elastic phase,DES as the liquid ionic conductive phase,and CNF as the reinforcement phase.The resulting eutectogel fibers exhibit excellent tensile strength(37.3 MPa),good elongation(>700%),high electrical conductivity(0.543 S/m),and resistance to extreme dry and-60°C low-tem-perature environments.Furthermore,these eutectogel fibers demonstrate high sensitivity for monitoring joint movements and effectively detecting in vitro and in vivo signals,show casing their potential for wearable strain sensors and monitoring physiological signals.展开更多
We constructed a eukaryotic expression plas-mid encoding Epstein-Barr virus latent membrane protein 2(EBV,LMP2)and evaluated its effects on humoral immunity.First,the encoding sequence of the EBV LMP2 was amplified fro...We constructed a eukaryotic expression plas-mid encoding Epstein-Barr virus latent membrane protein 2(EBV,LMP2)and evaluated its effects on humoral immunity.First,the encoding sequence of the EBV LMP2 was amplified from B95-8 cell RNA by reverse transcrip-tion polymerase chain reaction(RT-PCR)and then was directionally cloned into eukaryotic expression vector pcDNA3.1.It was employed to evaluate immune response of the mice inoculated doubly with the DNA vaccine.The serum antibody against LMP2 was detected with enzyme-linked immunosorbent assay(ELISA).The recombinant plasmid pcDNA3.1-LMP2 was confirmed by the restrictive endonuclease analysis and sequence analysis.The serum titer of IgG antibody against LMP2 epitope in the mice immunized with the DNA vaccine encoding LMP2 was up to 1∶4000.In conclusion,the EBV LMP2 DNA vaccine can induce a strong humoral immune response in mice.展开更多
基金supports from National Natural Science Foundation of China(32071346,32271403)Youth Innovation Promotion Association of the Chinese Academy of Sciences(2021364)+2 种基金Young Top-notch Talents Program of Guangdong Special Support Plan(2023TQ07A534)GuangDong Basic and Applied Basic Research Foundation(2024A1515010223)Engineering Technology R&D Center of Guangdong Provincial Universities(2024GCZX004).
文摘Flexible sensors are promising candidates in personalized healthcare,while desired sensors that allow implantation for biomedical applications with optimal sensing and favorable biological properties remain challenges.Here,a multifunctional hydrogel sensor was developed with gallic acid-modified chitosan(CSGA)and 3-carboxyphenylboronic acid-modified chitosan(CSPBA)by encapsulating Agdecorated polydopamine(Ag@PDA)nanoparticles,namely Ag@PDA-(CSPBA/CSGA).The optimized hydrogel sensor showed desired sensitivity(gauge factor=2.49),a rapid response/recovery time of 263 ms and good durability.Due to the presence of abundant reactive groups within Ag@PDA-(CSPBA/CSGA),the hydrogel sensor exhibited a comprehensive performance of self-healing,tissue adhesiveness,antioxidative activity,and antibacterial effects against Escherichia coli(92.76%)and Staphylococcus aureus(98.08%).Moreover,the hydrogel sensor could be utilized as a wound dressing,facilitating accelerated wound closure and tissue regeneration.Both subtle activities and large-scale movements could be monitored and distinguished by the hydrogel sensor.This study provides a promising epidermal sensor that offers multifunctionality for health monitoring and wound management.
基金National Natural Science Foundation of China,Grant/Award Numbers:22207019,22108001Basic and Applied Basic Research Project of Guangzhou Science and Technology Bureau,Grant/Award Number:SL2023A04J00117。
文摘The poor prognosis of triple-negative breast cancer(TNBC)results from its high metastasis,whereas inflammation accompanied by excessive reactive oxygen species(ROS)is prone to aggravate tumor metastasis.Although photothermal therapy(PTT)has extremely high therapeutic efficiency,the crafty tumor cells allow an increase in the expression of heat shock proteins(HSPs)to limit its effect,and PTT-induced inflammation is also thought to be a potential trigger for tumor metastasis.Herein,myricetin,iron ions,and polyvinylpyrrolidone were utilized to develop nanomedicines by self-assembly strategy for the treatment of metastatic TNBC.The nanomedicines with marvelous water solubility and dispersion can inhibit glucose transporter 1 and interfere with mitochondrial function to block the energy supply of tumor cells,achieving starvation therapy on TNBC cells.Nanomedicines with excellent photothermal conversion properties allow down-regulating the expression of HSPs to enhance the effect of PTT.Interestingly,the broad spectrum of ROS scavenging ability of nanomedicines successfully attenuates PTT-induced inflammation as well as influences hypoxia-inducible factors-1α/3-phosphoinositide-dependent protein kinase 1 related pathway through glycometabolism inhibition to reduce tumor cell metastasis.Moreover,the nanomedicines have negligible side effects and good clinical application prospects,which provides a valuable paradigm for the treatment of metastatic TNBC through glycometabolism interference,anti-inflammation,starvation,and photothermal synergistic therapy.
基金Supported by the Hundred Talents Program of Chinese Academy of Sciences(No.Y14408)Shenzhen Science and Technology Program(No.JC201005270293A,JC201104220257A and 201101017)National Natural Science Foundation of China(No.81471738)
文摘Objective： To explore brain activations associated with electroacupuncture simulation at Tongli（HT 5） and its comparison with brain activations during picture-naming task. Methods： Twenty healthy subjects were enrolled in this study. Half of them received electroacupuncture stimulation at HT 5（ACUP group） and the other half of them received stimulation at a nonmeridian sham acupoint（SHAM group）. All subjects performed picture-naming task. Each subject finished two runs of functional magnetic resonance imaging examinations in one session and picture-naming task was performed before electroacupuncture stimulation. Subjective brain activations were obtained using generalized linear model and inter-group analyses were performed after that. Results： The electroacupuncture stimulation at HT 5 induced significant brain activations in both the anterior and posterior language regions, including the left inferior frontal gyrus, which was in consistent with activations induced during picture-naming task. Group analysis showed a tendency of increased activation of ACUP group in left inferior frontal gyrus compared with SHAM group（P〈0.05 FDR corrected）. Conclusions： Electroacupuncture treatment at the acupoint HT 5 has modulation effect on typical language-implicated brain regions in healthy subjects, which provides supporting evidence for beneficial effects of needling at HT 5 for recovery of language function in aphasia.
基金the support of the National Key R&D Program of China(2023YFB3809902)National Natural Science Foundation of China(22108031)+1 种基金Shanghai Sailing Program(21YF1400800)the support of the Key Lab for Modification of Chemical Fibers&Polymer Materials,its Analysis Center and Lab.
文摘In recent years,the collection and monitoring of human physiological signals have garnered increasing attention due to their wide-ranging applications in healthcare,human-machine interaction,sports,and other fields.However,the continuous fabrication of flexible gel fiber electrodes with high mechanical performance,high conductivity,and durability for extreme environments using a simple,efficient,and universal strategy remains challenging for physiological signal acquisition.Here,we have employed a strategy of solvent replacement and multi-level hydrogen bond enhancement to construct eutectogel fibers with continuous solid-liquid structure,achieving continuous production of fibers with high strength,high conductivity,and low-temperature resistance.In the fiber,PVA serves as the solid-state elastic phase,DES as the liquid ionic conductive phase,and CNF as the reinforcement phase.The resulting eutectogel fibers exhibit excellent tensile strength(37.3 MPa),good elongation(>700%),high electrical conductivity(0.543 S/m),and resistance to extreme dry and-60°C low-tem-perature environments.Furthermore,these eutectogel fibers demonstrate high sensitivity for monitoring joint movements and effectively detecting in vitro and in vivo signals,show casing their potential for wearable strain sensors and monitoring physiological signals.
文摘We constructed a eukaryotic expression plas-mid encoding Epstein-Barr virus latent membrane protein 2(EBV,LMP2)and evaluated its effects on humoral immunity.First,the encoding sequence of the EBV LMP2 was amplified from B95-8 cell RNA by reverse transcrip-tion polymerase chain reaction(RT-PCR)and then was directionally cloned into eukaryotic expression vector pcDNA3.1.It was employed to evaluate immune response of the mice inoculated doubly with the DNA vaccine.The serum antibody against LMP2 was detected with enzyme-linked immunosorbent assay(ELISA).The recombinant plasmid pcDNA3.1-LMP2 was confirmed by the restrictive endonuclease analysis and sequence analysis.The serum titer of IgG antibody against LMP2 epitope in the mice immunized with the DNA vaccine encoding LMP2 was up to 1∶4000.In conclusion,the EBV LMP2 DNA vaccine can induce a strong humoral immune response in mice.
