A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3 O4@TiO2（Fe3 O4@mTiO2） microsphere and a 5-aminoisophthalic acid（AIPA） monolayer that provides additional ...A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3 O4@TiO2（Fe3 O4@mTiO2） microsphere and a 5-aminoisophthalic acid（AIPA） monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3 O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3 O4@mTiO2-AIPA was successfully utilized to separate α-casein（a typical phosphoprotein） and bovine serum albumin（BSA, a typical non-phosphoprotein） from their mixtures（molar ratio = 1:2）. Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3 O4@mTiO2-AIPA for application in phosphoprotein enrichment.展开更多
In this paper, the transparent hard diamond-like carbon (DLC) films were deposited on glass substrate by magnetic confined radio-frequency plasma chemical vapor deposition. The structure of films was studied by Raman ...In this paper, the transparent hard diamond-like carbon (DLC) films were deposited on glass substrate by magnetic confined radio-frequency plasma chemical vapor deposition. The structure of films was studied by Raman spectra and X-ray photoelectron spectra (XPS), the transmittance of films by Spectrophotometer. The mechanism of the influence of films structure on transmittance of the films was discussed. The results show that the thickness of films was lower than 100nm, and the transmittance was over 90% in 380-780 nm region. Discussion in theory on the influence of film structure on transmittance was correspondence to experiment results.展开更多
基金Funded by the National Natural Science Foundation of China(Nos.51473131,21275114,51533007 and 51521001)the Major State Basic Research Development Program of China(973 Program)(No.2013CB933002)+1 种基金Hubei Provincial Department of Education for Financial Assistance Through the “Chutian Scholar” ProgramHubei Provincial Natural Science Foundation of China(No.2014CFA039)
文摘A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3 O4@TiO2（Fe3 O4@mTiO2） microsphere and a 5-aminoisophthalic acid（AIPA） monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3 O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3 O4@mTiO2-AIPA was successfully utilized to separate α-casein（a typical phosphoprotein） and bovine serum albumin（BSA, a typical non-phosphoprotein） from their mixtures（molar ratio = 1:2）. Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3 O4@mTiO2-AIPA for application in phosphoprotein enrichment.
文摘In this paper, the transparent hard diamond-like carbon (DLC) films were deposited on glass substrate by magnetic confined radio-frequency plasma chemical vapor deposition. The structure of films was studied by Raman spectra and X-ray photoelectron spectra (XPS), the transmittance of films by Spectrophotometer. The mechanism of the influence of films structure on transmittance of the films was discussed. The results show that the thickness of films was lower than 100nm, and the transmittance was over 90% in 380-780 nm region. Discussion in theory on the influence of film structure on transmittance was correspondence to experiment results.
