利用可视化分析软件,客观、全面地展示和分析适体的研究现状、研究热点和发展趋势,为适体的研究提供指导和参考。以SCI数据库Web of Science平台为数据源,采用CiteSpace 5.5.R2软件对1900年1月1日至2020年12月31日的适体相关文献进行分...利用可视化分析软件,客观、全面地展示和分析适体的研究现状、研究热点和发展趋势,为适体的研究提供指导和参考。以SCI数据库Web of Science平台为数据源,采用CiteSpace 5.5.R2软件对1900年1月1日至2020年12月31日的适体相关文献进行分析。共纳入文献14939篇。在该领域发表论文最多的作者是WEIHONG TAN、KHALIL ABNOUS等;近年来突现的主题词是“wide linear range”“pg ml”“clinical diagnosis”“early diagnosis”等,是目前该领域的研究热点;作者共被引分析和文献共被引分析表明,该领域的研究主要集中在适体的体外筛选、适体构象的变化、适体在药物靶向治疗中的应用及适体生物传感器。结果表明,利用CiteSpace软件对Web of Science数据库中已发表的适体文献进行分析,揭示了该领域的研究热点从体外筛选和适体构象变化(1992—2002)到适体在药物靶向治疗和适体生物传感器中的应用(2002—2020)。结合近年来突现的主题词预测适体在药物靶向治疗和适体生物传感器中的应用仍将是未来的研究热点。展开更多
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global economic and health crisis. Recently, plasma levels of galectin-9 (Gal-9), a β-galactoside-binding lectin involved in immu...The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global economic and health crisis. Recently, plasma levels of galectin-9 (Gal-9), a β-galactoside-binding lectin involved in immune regulation and viral immunopathogenesis, were reported to be elevated in the setting of severe COVID-19 disease. However, the impact of Gal-9 on SARS-CoV-2 infection and immunopathology remained to be elucidated. In this study, we demonstrate that Gal-9 treatment potently enhances SARS-CoV-2 replication in human airway epithelial cells (AECs), including immortalized AECs and primary AECs cultured at the air–liquid interface. Gal-9–glycan interactions promote SARS-CoV-2 attachment and entry into AECs in an angiotensin-converting enzyme 2 (ACE2)-dependent manner, enhancing the binding of the viral spike protein to ACE2. Transcriptomic analysis revealed that Gal-9 and SARS-CoV-2 infection synergistically induced the expression of key pro-inflammatory programs in AECs, including the IL-6, IL-8, IL-17, EIF2, and TNFα signaling pathways. Our findings suggest that manipulation of Gal-9 should be explored as a therapeutic strategy for SARS-CoV-2 infection.展开更多
The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combine...The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System(CODIS)core STR loci,SE33,DYS391,and the standard sex-determining locus,amelogenin,as well as two special internal performance quality sensor controls(QS1 and QS2),which are included in the primer mix to check the PCR performance.This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions,sensitivity,precision,accuracy,repeatability,reproducibility,and concordance;tolerance to PCR inhibitors;applicability to real“forensic-type”samples;species specificity;mixture,balance and stutter analyses,and utility in a population investigation.The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate,sensitive and robust for STR genotyping.In addition,these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.展开更多
文摘利用可视化分析软件,客观、全面地展示和分析适体的研究现状、研究热点和发展趋势,为适体的研究提供指导和参考。以SCI数据库Web of Science平台为数据源,采用CiteSpace 5.5.R2软件对1900年1月1日至2020年12月31日的适体相关文献进行分析。共纳入文献14939篇。在该领域发表论文最多的作者是WEIHONG TAN、KHALIL ABNOUS等;近年来突现的主题词是“wide linear range”“pg ml”“clinical diagnosis”“early diagnosis”等,是目前该领域的研究热点;作者共被引分析和文献共被引分析表明,该领域的研究主要集中在适体的体外筛选、适体构象的变化、适体在药物靶向治疗中的应用及适体生物传感器。结果表明,利用CiteSpace软件对Web of Science数据库中已发表的适体文献进行分析,揭示了该领域的研究热点从体外筛选和适体构象变化(1992—2002)到适体在药物靶向治疗和适体生物传感器中的应用(2002—2020)。结合近年来突现的主题词预测适体在药物靶向治疗和适体生物传感器中的应用仍将是未来的研究热点。
基金supported by the Program for Breakthrough Biomedical Research,which is partially funded by the Sandler Foundation.Additional support was provided by the National Institutes of Health(R01MH112457 to S.K.P.)and the University of California San Francisco-Gladstone Institute of Virology&Immunology Center for AIDS Research(P30 Al027763).
文摘The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global economic and health crisis. Recently, plasma levels of galectin-9 (Gal-9), a β-galactoside-binding lectin involved in immune regulation and viral immunopathogenesis, were reported to be elevated in the setting of severe COVID-19 disease. However, the impact of Gal-9 on SARS-CoV-2 infection and immunopathology remained to be elucidated. In this study, we demonstrate that Gal-9 treatment potently enhances SARS-CoV-2 replication in human airway epithelial cells (AECs), including immortalized AECs and primary AECs cultured at the air–liquid interface. Gal-9–glycan interactions promote SARS-CoV-2 attachment and entry into AECs in an angiotensin-converting enzyme 2 (ACE2)-dependent manner, enhancing the binding of the viral spike protein to ACE2. Transcriptomic analysis revealed that Gal-9 and SARS-CoV-2 infection synergistically induced the expression of key pro-inflammatory programs in AECs, including the IL-6, IL-8, IL-17, EIF2, and TNFα signaling pathways. Our findings suggest that manipulation of Gal-9 should be explored as a therapeutic strategy for SARS-CoV-2 infection.
基金This study was supported by the General Program of National Natural Science Foundation of China[grant number 81625013 and 81772028]the Shanghai Outstanding Academic Leaders Plan[grant number 2017485]the Shanghai Talent Development Funding[grant number 2017115].
文摘The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System(CODIS)core STR loci,SE33,DYS391,and the standard sex-determining locus,amelogenin,as well as two special internal performance quality sensor controls(QS1 and QS2),which are included in the primer mix to check the PCR performance.This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions,sensitivity,precision,accuracy,repeatability,reproducibility,and concordance;tolerance to PCR inhibitors;applicability to real“forensic-type”samples;species specificity;mixture,balance and stutter analyses,and utility in a population investigation.The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate,sensitive and robust for STR genotyping.In addition,these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.
